Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043352.01.T01 | XP_013444960.1 | 88.793 | 116 | 13 | 0 | 1 | 116 | 1 | 116 | 5.88E-69 | 212 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043352.01.T01 | A0A072TZM0 | 88.793 | 116 | 13 | 0 | 1 | 116 | 1 | 116 | 2.81e-69 | 212 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480020433.01 | MsG0880043352.01 | 0.814589 | 1.426913e-51 | 9.613650e-49 |
| MsG0580029396.01 | MsG0880043352.01 | 0.801288 | 9.664839e-49 | 4.598161e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043352.01.T01 | MTR_8g032890 | 88.793 | 116 | 13 | 0 | 1 | 116 | 1 | 116 | 7.13e-73 | 212 |
| MsG0880043352.01.T01 | MTR_8g043660 | 86.207 | 116 | 16 | 0 | 1 | 116 | 1 | 116 | 7.30e-72 | 210 |
| MsG0880043352.01.T01 | MTR_8g032940 | 82.759 | 116 | 20 | 0 | 1 | 116 | 1 | 116 | 6.65e-60 | 179 |
| MsG0880043352.01.T01 | MTR_1g101680 | 60.417 | 96 | 32 | 2 | 18 | 112 | 6 | 96 | 5.12e-36 | 119 |
| MsG0880043352.01.T01 | MTR_1g060430 | 53.774 | 106 | 42 | 1 | 13 | 111 | 6 | 111 | 2.91e-34 | 115 |
| MsG0880043352.01.T01 | MTR_7g104250 | 55.670 | 97 | 36 | 2 | 17 | 111 | 1 | 92 | 1.89e-29 | 102 |
| MsG0880043352.01.T01 | MTR_1g101750 | 86.538 | 52 | 6 | 1 | 62 | 112 | 7 | 58 | 2.11e-27 | 96.3 |
| MsG0880043352.01.T01 | MTR_7g055590 | 43.925 | 107 | 14 | 3 | 2 | 107 | 431 | 492 | 4.43e-16 | 73.2 |
| MsG0880043352.01.T01 | MTR_5g038200 | 58.537 | 41 | 17 | 0 | 61 | 101 | 34 | 74 | 2.19e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043352.01.T01 | AT3G52790 | 50.000 | 106 | 49 | 1 | 11 | 112 | 3 | 108 | 8.51e-32 | 108 |
| MsG0880043352.01.T01 | AT5G62150 | 71.642 | 67 | 18 | 1 | 47 | 113 | 32 | 97 | 8.50e-31 | 105 |
| MsG0880043352.01.T01 | AT4G25433 | 49.438 | 89 | 35 | 1 | 31 | 109 | 16 | 104 | 1.36e-25 | 92.8 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTTGTTGTGTAATTTAT+AGG | 0.189718 | 8:-23499830 | None:intergenic |
| GTGTTATGATCTTGATTATT+TGG | 0.200053 | 8:+23500061 | None:intergenic |
| CAAGAAATGGTTGCTTTCTA+TGG | 0.286339 | 8:+23500039 | None:intergenic |
| AAAGAACAATAATAAATTCT+AGG | 0.312259 | 8:+23499847 | None:intergenic |
| CAAAGAGCTGTGTGTAAGTA+TGG | 0.322118 | 8:+23500087 | None:intergenic |
| GGATTATTCTCAACAATAAA+AGG | 0.331253 | 8:+23499938 | None:intergenic |
| CCATAGCTGATGCAACTTCA+TGG | 0.375379 | 8:-23500147 | MsG0880043352.01.T01:CDS |
| GACCCTGATGATGTCTTCCC+TGG | 0.397220 | 8:-23499905 | MsG0880043352.01.T01:CDS |
| AGGCCAGGGAAGACATCATC+AGG | 0.409337 | 8:+23499902 | None:intergenic |
| ATGGTTTCGAAGACAAGAAA+TGG | 0.448480 | 8:+23500026 | None:intergenic |
| GGTTTCTTTCAACTCAAGAA+AGG | 0.470711 | 8:-23500191 | MsG0880043352.01.T01:CDS |
| TCATCAGGGTCATGAATATG+AGG | 0.504482 | 8:+23499917 | None:intergenic |
| GTTTCTTTCAACTCAAGAAA+GGG | 0.513167 | 8:-23500190 | MsG0880043352.01.T01:CDS |
| TTCTAGGTATGATGATATGA+AGG | 0.540443 | 8:+23499863 | None:intergenic |
| GTAATCTTGATAACAAGGCC+AGG | 0.555624 | 8:+23499887 | None:intergenic |
| CCATGAAGTTGCATCAGCTA+TGG | 0.557482 | 8:+23500147 | None:intergenic |
| ACCACATAGATTTCGTCGCA+TGG | 0.594621 | 8:+23500007 | None:intergenic |
| GAAATCTATGTGGTTGGTGA+AGG | 0.606236 | 8:-23499998 | MsG0880043352.01.T01:CDS |
| TAATCTTGATAACAAGGCCA+GGG | 0.625062 | 8:+23499888 | None:intergenic |
| AAGGTGTAATCTTGATAACA+AGG | 0.626690 | 8:+23499882 | None:intergenic |
| TGCGACGAAATCTATGTGGT+TGG | 0.672034 | 8:-23500004 | MsG0880043352.01.T01:CDS |
| ACCATGCGACGAAATCTATG+TGG | 0.683872 | 8:-23500008 | MsG0880043352.01.T01:CDS |
| GGCCAGGGAAGACATCATCA+GGG | 0.697276 | 8:+23499903 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAACAATAATAAATTCT+AGG | + | Chr8:23500180-23500199 | None:intergenic | 15.0% |
| ! | GGATTATTCTCAACAATAAA+AGG | + | Chr8:23500089-23500108 | None:intergenic | 25.0% |
| ! | GTGTTATGATCTTGATTATT+TGG | + | Chr8:23499966-23499985 | None:intergenic | 25.0% |
| GTTTCTTTCAACTCAAGAAA+GGG | - | Chr8:23499834-23499853 | MsG0880043352.01.T02:CDS | 30.0% | |
| TTCTAGGTATGATGATATGA+AGG | + | Chr8:23500164-23500183 | None:intergenic | 30.0% | |
| ! | AAGGTGTAATCTTGATAACA+AGG | + | Chr8:23500145-23500164 | None:intergenic | 30.0% |
| ATGGTTTCGAAGACAAGAAA+TGG | + | Chr8:23500001-23500020 | None:intergenic | 35.0% | |
| GGTTTCTTTCAACTCAAGAA+AGG | - | Chr8:23499833-23499852 | MsG0880043352.01.T02:CDS | 35.0% | |
| ! | CAAGAAATGGTTGCTTTCTA+TGG | + | Chr8:23499988-23500007 | None:intergenic | 35.0% |
| ! | TAATCTTGATAACAAGGCCA+GGG | + | Chr8:23500139-23500158 | None:intergenic | 35.0% |
| CAAAGAGCTGTGTGTAAGTA+TGG | + | Chr8:23499940-23499959 | None:intergenic | 40.0% | |
| TCATCAGGGTCATGAATATG+AGG | + | Chr8:23500110-23500129 | None:intergenic | 40.0% | |
| ! | GTAATCTTGATAACAAGGCC+AGG | + | Chr8:23500140-23500159 | None:intergenic | 40.0% |
| !! | GAAATCTATGTGGTTGGTGA+AGG | - | Chr8:23500026-23500045 | MsG0880043352.01.T01:CDS | 40.0% |
| ACCACATAGATTTCGTCGCA+TGG | + | Chr8:23500020-23500039 | None:intergenic | 45.0% | |
| ACCATGCGACGAAATCTATG+TGG | - | Chr8:23500016-23500035 | MsG0880043352.01.T01:CDS | 45.0% | |
| CCATAGCTGATGCAACTTCA+TGG | - | Chr8:23499877-23499896 | MsG0880043352.01.T01:CDS | 45.0% | |
| CCATGAAGTTGCATCAGCTA+TGG | + | Chr8:23499880-23499899 | None:intergenic | 45.0% | |
| TGCGACGAAATCTATGTGGT+TGG | - | Chr8:23500020-23500039 | MsG0880043352.01.T01:CDS | 45.0% | |
| AGGCCAGGGAAGACATCATC+AGG | + | Chr8:23500125-23500144 | None:intergenic | 55.0% | |
| GGCCAGGGAAGACATCATCA+GGG | + | Chr8:23500124-23500143 | None:intergenic | 55.0% | |
| ! | GACCCTGATGATGTCTTCCC+TGG | - | Chr8:23500119-23500138 | MsG0880043352.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 23499831 | 23500215 | 23499831 | ID=MsG0880043352.01;Name=MsG0880043352.01 |
| Chr8 | mRNA | 23499865 | 23500215 | 23499865 | ID=MsG0880043352.01.T01;Parent=MsG0880043352.01;Name=MsG0880043352.01.T01;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|0|1|0|116 |
| Chr8 | exon | 23499865 | 23500215 | 23499865 | ID=MsG0880043352.01.T01:exon:37526;Parent=MsG0880043352.01.T01 |
| Chr8 | CDS | 23499865 | 23500215 | 23499865 | ID=MsG0880043352.01.T01:cds;Parent=MsG0880043352.01.T01 |
| Chr8 | mRNA | 23499831 | 23500013 | 23499831 | ID=MsG0880043352.01.T02;Parent=MsG0880043352.01;Name=MsG0880043352.01.T02;_AED=0.33;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|60 |
| Chr8 | exon | 23499831 | 23500013 | 23499831 | ID=MsG0880043352.01.T02:exon:37527;Parent=MsG0880043352.01.T02 |
| Chr8 | CDS | 23499831 | 23500013 | 23499831 | ID=MsG0880043352.01.T02:cds;Parent=MsG0880043352.01.T02 |
| Gene Sequence |
| Protein sequence |
