AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043618.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043618.01.T01 MTR_8g039110 93.884 327 18 1 1 325 1 327 0.0 590
MsG0880043618.01.T01 MTR_0363s0040 91.667 216 16 1 1 214 1 216 1.49e-126 362
MsG0880043618.01.T01 MTR_3g014660 60.933 343 100 16 1 324 1 328 3.45e-112 328
MsG0880043618.01.T01 MTR_5g089750 44.074 270 124 8 42 296 159 416 4.83e-51 176
MsG0880043618.01.T01 MTR_8g076620 40.698 172 73 2 109 280 108 250 5.51e-34 127
MsG0880043618.01.T01 MTR_5g021130 44.099 161 83 2 122 282 100 253 8.84e-33 124
MsG0880043618.01.T01 MTR_3g112510 38.710 186 82 6 122 302 86 244 9.34e-30 115
MsG0880043618.01.T01 MTR_4g071110 36.782 174 72 4 111 284 6 141 7.52e-25 101
MsG0880043618.01.T01 MTR_1g023230 75.556 45 11 0 122 166 122 166 4.06e-18 82.8
MsG0880043618.01.T01 MTR_0105s0070 63.636 33 12 0 124 156 44 76 1.51e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043618.01.T01 AT5G12330 39.077 325 176 8 1 320 1 308 3.10e-56 185
MsG0880043618.01.T01 AT5G12330 39.077 325 176 8 1 320 1 308 3.10e-56 185
MsG0880043618.01.T01 AT5G12330 39.077 325 176 8 1 320 88 395 9.26e-56 186
MsG0880043618.01.T01 AT3G54430 44.571 175 65 4 120 293 39 182 1.86e-38 135
MsG0880043618.01.T01 AT1G75520 44.444 162 83 3 122 280 125 282 2.10e-37 137
MsG0880043618.01.T01 AT5G66350 43.575 179 83 3 109 287 107 267 7.90e-37 135
MsG0880043618.01.T01 AT5G66350 43.575 179 83 3 109 287 107 267 8.79e-37 135
MsG0880043618.01.T01 AT5G66350 43.575 179 83 3 109 287 107 267 1.04e-36 134
MsG0880043618.01.T01 AT3G51060 45.625 160 63 4 122 278 144 282 1.22e-34 130
MsG0880043618.01.T01 AT1G19790 43.210 162 78 3 122 280 119 269 3.13e-33 125
MsG0880043618.01.T01 AT1G19790 43.210 162 78 3 122 280 119 269 3.13e-33 125
MsG0880043618.01.T01 AT4G36260 33.921 227 131 5 59 280 32 244 3.34e-30 117
MsG0880043618.01.T01 AT2G21400 38.272 162 71 4 120 281 8 140 1.69e-29 112
MsG0880043618.01.T01 AT2G21400 38.272 162 71 4 120 281 7 139 1.70e-29 112
MsG0880043618.01.T01 AT2G18120 37.195 164 73 3 122 284 72 206 2.03e-29 112
MsG0880043618.01.T01 AT2G18120 36.810 163 77 3 122 284 72 208 2.30e-29 112
MsG0880043618.01.T01 AT2G21400 35.802 162 60 2 120 281 8 125 8.16e-28 107
MsG0880043618.01.T01 AT2G21400 35.802 162 60 2 120 281 7 124 8.92e-28 107
MsG0880043618.01.T01 AT5G12330 37.089 213 117 6 1 208 1 201 8.22e-27 105
MsG0880043618.01.T01 AT5G33210 70.000 50 15 0 122 171 17 66 2.81e-20 84.7
MsG0880043618.01.T01 AT5G33210 70.000 50 15 0 122 171 52 101 2.99e-19 84.0

Find 85 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATAGAATAGGGAGATTT+TGG 0.131186 8:-27844120 None:intergenic
TGCTTGTTGAGATTGCTTTA+AGG 0.200707 8:+27845795 MsG0880043618.01.T01:exon
TGAAGAAAATGGTGAGTTTA+GGG 0.286532 8:+27844633 MsG0880043618.01.T01:CDS
ATATTCTCATTGTTATTGTT+AGG 0.304242 8:-27844481 None:intergenic
AGTAGCATTGAGAATAGAAT+AGG 0.306600 8:-27844130 None:intergenic
GTGAAGAAAATGGTGAGTTT+AGG 0.324594 8:+27844632 MsG0880043618.01.T01:CDS
CTATTAGAACCAAGTCTCTT+AGG 0.357173 8:-27844309 None:intergenic
GTAGCATTGAGAATAGAATA+GGG 0.371162 8:-27844129 None:intergenic
TGAAGATGAACTTGCTTACT+TGG 0.373159 8:+27845591 MsG0880043618.01.T01:CDS
AGTGATGGTGGTGCTTCTTC+TGG 0.380721 8:+27844820 MsG0880043618.01.T01:CDS
TGTTGCAAAATGAACATGTT+AGG 0.386699 8:+27844286 MsG0880043618.01.T01:exon
GATGAAGGTAAGGGAATGTT+TGG 0.393591 8:-27844391 None:intergenic
TATTCTCATTGTTATTGTTA+GGG 0.402170 8:-27844480 None:intergenic
GAACTTAGATATCGATGTTA+CGG 0.405757 8:+27847771 MsG0880043618.01.T01:three_prime_UTR
ATGTTCTTCTTCTGCAATTG+AGG 0.412235 8:+27845747 MsG0880043618.01.T01:CDS
TCATCATTATTTGTTGTGGT+TGG 0.418207 8:-27844540 None:intergenic
AACTTAGATATCGATGTTAC+GGG 0.420068 8:+27847772 MsG0880043618.01.T01:three_prime_UTR
TGCCAACAGCAGATACTCTA+TGG 0.431824 8:-27845564 None:intergenic
CGGCGTCGATGAGGCGGGAC+CGG 0.436701 8:+27844770 MsG0880043618.01.T01:CDS
ATGTGTGTGTGATTGTGATT+TGG 0.443717 8:-27844176 None:intergenic
TGTTGGTTGTGAAGTGATGA+AGG 0.446919 8:-27844340 None:intergenic
TGGATTATGATCTGATGAAA+TGG 0.452099 8:-27844371 None:intergenic
TATGATCTGATGAAATGGTT+TGG 0.460879 8:-27844366 None:intergenic
TTTAGGGTTTGTCAAGATTG+TGG 0.461202 8:+27844649 MsG0880043618.01.T01:CDS
GAATTGCAACTTGGAAGTAA+TGG 0.469782 8:+27845703 MsG0880043618.01.T01:CDS
CAATCTCAACAAGCAGAAGC+AGG 0.470698 8:-27845787 None:intergenic
AATGTAATGTAAAGAGAGGA+TGG 0.472001 8:-27844228 None:intergenic
TAGTGCTGATGATGAAGGTA+AGG 0.474808 8:-27844401 None:intergenic
CAGAAAGGCACCACTGCTAC+AGG 0.479617 8:-27844095 None:intergenic
ATGATGGGGTGTGAAGAAAA+TGG 0.479641 8:+27844622 MsG0880043618.01.T01:CDS
TCAGCACTAAGTGTTGGTCT+TGG 0.487088 8:+27844415 MsG0880043618.01.T01:CDS
AAAGGGTTTCTCTATGATCA+AGG 0.488489 8:+27845643 MsG0880043618.01.T01:CDS
CATGTTAGGCCTAAGAGACT+TGG 0.493330 8:+27844300 MsG0880043618.01.T01:CDS
GTAGGATTTCCATGCATAAC+CGG 0.497416 8:-27847678 None:intergenic
GAAGCAGGGTAAGCACTAGC+TGG 0.498464 8:-27845772 None:intergenic
AATCAAAATTCTCTGCCTCT+TGG 0.499143 8:-27844845 None:intergenic
CGTGAAGAGCACGTGGATAC+CGG 0.501925 8:+27844750 MsG0880043618.01.T01:CDS
CCAAAGAAAGAATATGTAAG+TGG 0.507300 8:+27844050 None:intergenic
TTCATCATCATTATTTGTTG+TGG 0.510215 8:-27844544 None:intergenic
ATTGTAGCTATAAAAGATGC+AGG 0.519421 8:+27844689 MsG0880043618.01.T01:CDS
CAACTTGGAAGTAATGGAAG+TGG 0.519956 8:+27845709 MsG0880043618.01.T01:CDS
GTTCTTTCTCCGGTTATGCA+TGG 0.525100 8:+27847669 MsG0880043618.01.T01:three_prime_UTR
GGTGAGTAGTGGTTAGGAGT+GGG 0.529385 8:-27844444 None:intergenic
TAAGTGGCCATGTATTCAAA+GGG 0.529963 8:+27845626 MsG0880043618.01.T01:CDS
GGCAAAGTCTGCTTGAAACC+AGG 0.531084 8:-27845508 None:intergenic
AGAGAAACCCTTTGAATACA+TGG 0.531479 8:-27845633 None:intergenic
ATGGCAGGTAGTTCTTTCTC+CGG 0.531683 8:+27847659 MsG0880043618.01.T01:intron
ATAAGTGGCCATGTATTCAA+AGG 0.532258 8:+27845625 MsG0880043618.01.T01:CDS
TTGGCTACTGTGAATATAAG+TGG 0.534470 8:+27845610 MsG0880043618.01.T01:CDS
GCAGGCGCGCGAACATAGCG+AGG 0.545668 8:-27845529 None:intergenic
AGGACTTGTTGTAAAGGTCG+TGG 0.546270 8:+27844709 MsG0880043618.01.T01:CDS
GATGAGGGTGAGTAGTGGTT+AGG 0.546291 8:-27844450 None:intergenic
AGATGCAGGACTTGTTGTAA+AGG 0.546985 8:+27844703 MsG0880043618.01.T01:CDS
TCATCATCACAGAATAATGC+TGG 0.553164 8:+27844868 MsG0880043618.01.T01:CDS
TACATAACAACAAGTACTGT+AGG 0.554569 8:-27847696 None:intergenic
GCTTAAACTTCTTGTTCTAG+TGG 0.556742 8:-27844923 None:intergenic
CTATGGCACTTGAAAACAGC+AGG 0.559201 8:-27845547 None:intergenic
GGGTGAGTAGTGGTTAGGAG+TGG 0.563980 8:-27844445 None:intergenic
GATACCGGCGTCGATGAGGC+GGG 0.565411 8:+27844765 MsG0880043618.01.T01:CDS
GGAGAATGTAATGTAAAGAG+AGG 0.573573 8:-27844232 None:intergenic
ACACTTAGTGCTGATGATGA+AGG 0.573973 8:-27844406 None:intergenic
TGTGTTTCAGAATTGCAACT+TGG 0.580875 8:+27845694 MsG0880043618.01.T01:CDS
TGCCATAGAGTATCTGCTGT+TGG 0.581569 8:+27845562 MsG0880043618.01.T01:CDS
TTGATGATGAGGGTGAGTAG+TGG 0.583936 8:-27844455 None:intergenic
GAGGCGGGACCGGAGTATGA+TGG 0.586244 8:+27844780 MsG0880043618.01.T01:CDS
CGGAGTATGATGGTGGCTGA+AGG 0.587151 8:+27844790 MsG0880043618.01.T01:CDS
GCTGAAGGTGGTGACAGTGA+TGG 0.591111 8:+27844805 MsG0880043618.01.T01:CDS
GGATACCGGCGTCGATGAGG+CGG 0.591264 8:+27844764 MsG0880043618.01.T01:CDS
GAAGGTGGTGACAGTGATGG+TGG 0.597594 8:+27844808 MsG0880043618.01.T01:CDS
ATGTAATGTAAAGAGAGGAT+GGG 0.606141 8:-27844227 None:intergenic
CGTGGATACCGGCGTCGATG+AGG 0.610218 8:+27844761 MsG0880043618.01.T01:CDS
AGAGTATCTGCTGTTGGCAA+TGG 0.611315 8:+27845568 MsG0880043618.01.T01:CDS
TCATCATCAGCACTAAGTGT+TGG 0.620780 8:+27844409 MsG0880043618.01.T01:CDS
AGTGCTGATGATGAAGGTAA+GGG 0.621262 8:-27844400 None:intergenic
AATCTCAACAAGCAGAAGCA+GGG 0.625538 8:-27845786 None:intergenic
GTTGTGGTTGGCACATCTTG+AGG 0.627215 8:-27844528 None:intergenic
CGGTCCCGCCTCATCGACGC+CGG 0.629933 8:-27844769 None:intergenic
GCGGGACCGGAGTATGATGG+TGG 0.640592 8:+27844783 MsG0880043618.01.T01:CDS
AGAATTCAAACCTGTAGCAG+TGG 0.640764 8:+27844085 MsG0880043618.01.T01:five_prime_UTR
GTGCTTCTTCTGGTACCAAG+AGG 0.651535 8:+27844830 MsG0880043618.01.T01:CDS
CTTCAGCCACCATCATACTC+CGG 0.656848 8:-27844789 None:intergenic
AGTATGATGGTGGCTGAAGG+TGG 0.658861 8:+27844793 MsG0880043618.01.T01:CDS
ATAACTAGCTCTTGTCACCA+AGG 0.669802 8:+27844952 MsG0880043618.01.T01:CDS
GTACTCACGTGAAGAGCACG+TGG 0.684544 8:+27844743 MsG0880043618.01.T01:CDS
AGTTGCAATTCTGAAACACA+AGG 0.685469 8:-27845691 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTAAATAGTTAAATATA+CGG + Chr8:27846632-27846651 MsG0880043618.01.T01:intron 10.0%
!! TATATTAAAAATTATGTGAA+TGG - Chr8:27846963-27846982 None:intergenic 10.0%
!! TTAATATGATTATTTAATCA+TGG - Chr8:27844995-27845014 None:intergenic 10.0%
!!! ATTTAATGTTTTTTTTTTCT+AGG + Chr8:27847802-27847821 MsG0880043618.01.T01:three_prime_UTR 10.0%
!!! TTGTTATTTCATAAATTTAA+GGG + Chr8:27845239-27845258 MsG0880043618.01.T01:intron 10.0%
!!! TTTAATGTTTTTTTTTTCTA+GGG + Chr8:27847803-27847822 MsG0880043618.01.T01:three_prime_UTR 10.0%
!!! TTTGTTATTTCATAAATTTA+AGG + Chr8:27845238-27845257 MsG0880043618.01.T01:intron 10.0%
!! ATTAAAAAAAGTGAGTAAAT+AGG - Chr8:27845308-27845327 None:intergenic 15.0%
!! TTGAAATTGTATTCTTATTA+TGG + Chr8:27845942-27845961 MsG0880043618.01.T01:intron 15.0%
!!! AATATTTTTTTTTCTTCTGA+TGG + Chr8:27847640-27847659 MsG0880043618.01.T01:intron 15.0%
!!! GTTTACAAAACTTTTTTTTA+TGG + Chr8:27845021-27845040 MsG0880043618.01.T01:intron 15.0%
!!! TATTTAAAAGTGTCTAATTA+AGG + Chr8:27846716-27846735 MsG0880043618.01.T01:intron 15.0%
!!! TCATCTCATTATATATTTTT+GGG + Chr8:27847862-27847881 MsG0880043618.01.T01:three_prime_UTR 15.0%
!!! TTCATCTCATTATATATTTT+TGG + Chr8:27847861-27847880 MsG0880043618.01.T01:three_prime_UTR 15.0%
!! ATCTAAGGAATTGAAAAATT+AGG - Chr8:27846418-27846437 None:intergenic 20.0%
!! GAAAACTAAAACAATAGAAA+AGG + Chr8:27846042-27846061 MsG0880043618.01.T01:intron 20.0%
!! GTCAATAACTAGTAATTTAA+TGG - Chr8:27846461-27846480 None:intergenic 20.0%
!! TAGCTACAATAATATGTAAT+GGG - Chr8:27847471-27847490 None:intergenic 20.0%
!!! ATATTCTCATTGTTATTGTT+AGG - Chr8:27844484-27844503 None:intergenic 20.0%
!!! ATTTCGTCACATTTATTTTT+TGG + Chr8:27847057-27847076 MsG0880043618.01.T01:intron 20.0%
!!! CAATTCATAAAGATTTTTGT+AGG + Chr8:27847582-27847601 MsG0880043618.01.T01:intron 20.0%
!!! GTTATGTATTGTTTTCTTTA+GGG - Chr8:27844157-27844176 None:intergenic 20.0%
!!! GTTGGTTTTGTTTATTTATT+GGG - Chr8:27844256-27844275 None:intergenic 20.0%
!!! TATTCTCATTGTTATTGTTA+GGG - Chr8:27844483-27844502 None:intergenic 20.0%
!!! TCATTGTTACTTTATAAACT+AGG + Chr8:27845331-27845350 MsG0880043618.01.T01:intron 20.0%
!!! TGTTGGTTTTGTTTATTTAT+TGG - Chr8:27844257-27844276 None:intergenic 20.0%
!!! TTATGTATTGTTTTCTTTAG+GGG - Chr8:27844156-27844175 None:intergenic 20.0%
!!! TTTTTCTATTAACCAAACTT+AGG - Chr8:27845387-27845406 None:intergenic 20.0%
! AAAATAAATGTGACGAAATG+AGG - Chr8:27847056-27847075 None:intergenic 25.0%
! AAAGTTGTTACCTAAACATT+AGG - Chr8:27847495-27847514 None:intergenic 25.0%
! AAGAAACAAAAACTTGATGA+TGG + Chr8:27844606-27844625 MsG0880043618.01.T01:CDS 25.0%
! AAGTTGTTACCTAAACATTA+GGG - Chr8:27847494-27847513 None:intergenic 25.0%
! AATTGTGTATATCCTAAGTT+TGG + Chr8:27845372-27845391 MsG0880043618.01.T01:intron 25.0%
! ACAGTAGGTTGTTAAAATAT+AGG + Chr8:27846599-27846618 MsG0880043618.01.T01:intron 25.0%
! ACTAATTTGATACTAGTAAG+TGG + Chr8:27846557-27846576 MsG0880043618.01.T01:intron 25.0%
! AGTAGGTTGTTAAAATATAG+GGG + Chr8:27846601-27846620 MsG0880043618.01.T01:intron 25.0%
! ATATTGACATCATTTCCTAA+TGG - Chr8:27846117-27846136 None:intergenic 25.0%
! ATCATTTCCTAATGGTAAAA+TGG - Chr8:27846109-27846128 None:intergenic 25.0%
! CAGTAGGTTGTTAAAATATA+GGG + Chr8:27846600-27846619 MsG0880043618.01.T01:intron 25.0%
! GTAACATAGAAAACTTACTT+TGG - Chr8:27846820-27846839 None:intergenic 25.0%
! GTAGCTACAATAATATGTAA+TGG - Chr8:27847472-27847491 None:intergenic 25.0%
! GTATTGTTACAATGATAAGT+TGG + Chr8:27845861-27845880 MsG0880043618.01.T01:intron 25.0%
! TCAATAAACCAATCAATCTA+AGG - Chr8:27846433-27846452 None:intergenic 25.0%
! TGTATACTTGTGTCAATTTA+TGG + Chr8:27846773-27846792 MsG0880043618.01.T01:intron 25.0%
! TTCAAAACAACCACAAAATT+TGG - Chr8:27846842-27846861 None:intergenic 25.0%
! TTTCAATTCCTTAGATTGAT+TGG + Chr8:27846422-27846441 MsG0880043618.01.T01:intron 25.0%
!! AGAAACAAAAACTTGATGAT+GGG + Chr8:27844607-27844626 MsG0880043618.01.T01:CDS 25.0%
!! ATAGAAAACTTACTTTGGAA+GGG - Chr8:27846815-27846834 None:intergenic 25.0%
!! ATCATTTGTACGACAATTTT+TGG + Chr8:27845980-27845999 MsG0880043618.01.T01:intron 25.0%
!! ATGTTTTCATTGATGAAGTT+TGG + Chr8:27847008-27847027 MsG0880043618.01.T01:intron 25.0%
!! CATTTTACCATTTTACCATT+AGG + Chr8:27846099-27846118 MsG0880043618.01.T01:intron 25.0%
!! CTTCATACATTTTACCAAAT+CGG + Chr8:27847240-27847259 MsG0880043618.01.T01:intron 25.0%
!! GGTTATGTATTGTTTTCTTT+AGG - Chr8:27844158-27844177 None:intergenic 25.0%
!! TCATTTGTACGACAATTTTT+GGG + Chr8:27845981-27846000 MsG0880043618.01.T01:intron 25.0%
!! TTCATCATCATTATTTGTTG+TGG - Chr8:27844547-27844566 None:intergenic 25.0%
!! TTCTATGTTACCAAATTTTG+TGG + Chr8:27846829-27846848 MsG0880043618.01.T01:intron 25.0%
!!! ATATATTTTTGGGCTTTAGA+CGG + Chr8:27847872-27847891 MsG0880043618.01.T01:three_prime_UTR 25.0%
!!! TTAAAAGTGTCTAATTAAGG+CGG + Chr8:27846719-27846738 MsG0880043618.01.T01:intron 25.0%
AAATTGACTGGAGAAAATCA+TGG + Chr8:27847434-27847453 MsG0880043618.01.T01:intron 30.0%
AACAATACAAGTACACCAAA+AGG - Chr8:27845849-27845868 None:intergenic 30.0%
AACTTAGATATCGATGTTAC+GGG + Chr8:27847772-27847791 MsG0880043618.01.T01:three_prime_UTR 30.0%
AATGTAATGTAAAGAGAGGA+TGG - Chr8:27844231-27844250 None:intergenic 30.0%
ACAATACAAGTACACCAAAA+GGG - Chr8:27845848-27845867 None:intergenic 30.0%
AGTCATTGAAAGAATATGCT+AGG + Chr8:27846533-27846552 MsG0880043618.01.T01:intron 30.0%
ATCAATCAATTTAGTCTCAG+AGG - Chr8:27845198-27845217 None:intergenic 30.0%
ATGTAATGTAAAGAGAGGAT+GGG - Chr8:27844230-27844249 None:intergenic 30.0%
ATGTCACCTTCAATCAAAAA+AGG + Chr8:27846272-27846291 MsG0880043618.01.T01:intron 30.0%
ATTGTAGCTATAAAAGATGC+AGG + Chr8:27844689-27844708 MsG0880043618.01.T01:CDS 30.0%
CATAGAAAACTTACTTTGGA+AGG - Chr8:27846816-27846835 None:intergenic 30.0%
CGGATAATGATTTACAGAAA+TGG + Chr8:27845099-27845118 MsG0880043618.01.T01:intron 30.0%
GAACTTAGATATCGATGTTA+CGG + Chr8:27847771-27847790 MsG0880043618.01.T01:three_prime_UTR 30.0%
GAATCCAAAAAATGCAACAA+AGG - Chr8:27846165-27846184 None:intergenic 30.0%
TACTAGTAAGTGGATAAACT+CGG + Chr8:27846567-27846586 MsG0880043618.01.T01:intron 30.0%
TATGATCTGATGAAATGGTT+TGG - Chr8:27844369-27844388 None:intergenic 30.0%
TATTGAGCTATAGCAATGAA+TGG - Chr8:27847157-27847176 None:intergenic 30.0%
TGAAAATGTCCTAGTTTCTA+AGG - Chr8:27847093-27847112 None:intergenic 30.0%
TGAAGAAAATGGTGAGTTTA+GGG + Chr8:27844633-27844652 MsG0880043618.01.T01:CDS 30.0%
TGGATTATGATCTGATGAAA+TGG - Chr8:27844374-27844393 None:intergenic 30.0%
TGTTGCAAAATGAACATGTT+AGG + Chr8:27844286-27844305 MsG0880043618.01.T01:exon 30.0%
! AACATGTTCATTTTGCAACA+AGG - Chr8:27844286-27844305 None:intergenic 30.0%
! AGAATAGAATAGGGAGATTT+TGG - Chr8:27844123-27844142 None:intergenic 30.0%
! AGTAGCATTGAGAATAGAAT+AGG - Chr8:27844133-27844152 None:intergenic 30.0%
! GATATCTAATTAGACCCTTT+TGG + Chr8:27845831-27845850 MsG0880043618.01.T01:intron 30.0%
! GTAGCATTGAGAATAGAATA+GGG - Chr8:27844132-27844151 None:intergenic 30.0%
! TACATAACAACAAGTACTGT+AGG - Chr8:27847699-27847718 None:intergenic 30.0%
! TAGAAAACTTACTTTGGAAG+GGG - Chr8:27846814-27846833 None:intergenic 30.0%
! TTCATTTTGCAACAAGGTTT+TGG - Chr8:27844280-27844299 None:intergenic 30.0%
!! AACAAAAAGTGAAAGTACCT+TGG - Chr8:27844972-27844991 None:intergenic 30.0%
!! AATATGTAATGGGCAAAAAG+TGG - Chr8:27847461-27847480 None:intergenic 30.0%
!! GAAACAAAAACTTGATGATG+GGG + Chr8:27844608-27844627 MsG0880043618.01.T01:CDS 30.0%
!! TCATCATTATTTGTTGTGGT+TGG - Chr8:27844543-27844562 None:intergenic 30.0%
!!! AGTTGTCCTTTTTTGATTGA+AGG - Chr8:27846281-27846300 None:intergenic 30.0%
!!! ATGAAATGGTTTGGTTTTGT+TGG - Chr8:27844360-27844379 None:intergenic 30.0%
!!! TATATTTTTGGGCTTTAGAC+GGG + Chr8:27847873-27847892 MsG0880043618.01.T01:three_prime_UTR 30.0%
!!! TTTTTTTTTCTTCTGATGGC+AGG + Chr8:27847644-27847663 MsG0880043618.01.T01:intron 30.0%
AAAGGGTTTCTCTATGATCA+AGG + Chr8:27845643-27845662 MsG0880043618.01.T01:CDS 35.0%
AATCAAAATTCTCTGCCTCT+TGG - Chr8:27844848-27844867 None:intergenic 35.0%
AATCTGATTCAGCAAGAGTT+CGG + Chr8:27846390-27846409 MsG0880043618.01.T01:intron 35.0%
ACTAGTAAGTGGATAAACTC+GGG + Chr8:27846568-27846587 MsG0880043618.01.T01:intron 35.0%
AGACTCATCCAAGACAAATA+TGG - Chr8:27847412-27847431 None:intergenic 35.0%
AGAGAAACCCTTTGAATACA+TGG - Chr8:27845636-27845655 None:intergenic 35.0%
ATAAGTGGCCATGTATTCAA+AGG + Chr8:27845625-27845644 MsG0880043618.01.T01:CDS 35.0%
ATGTGTGTGTGATTGTGATT+TGG - Chr8:27844179-27844198 None:intergenic 35.0%
ATGTTCTTCTTCTGCAATTG+AGG + Chr8:27845747-27845766 MsG0880043618.01.T01:CDS 35.0%
CAACATGACTATTTGCCATT+TGG + Chr8:27847185-27847204 MsG0880043618.01.T01:intron 35.0%
CTATTAGAACCAAGTCTCTT+AGG - Chr8:27844312-27844331 None:intergenic 35.0%
CTGTAAATCATTATCCGTGT+CGG - Chr8:27845096-27845115 None:intergenic 35.0%
GAATTGCAACTTGGAAGTAA+TGG + Chr8:27845703-27845722 MsG0880043618.01.T01:CDS 35.0%
GCATAATCCAGTGTAATCAT+AGG + Chr8:27845121-27845140 MsG0880043618.01.T01:intron 35.0%
GCTTAAACTTCTTGTTCTAG+TGG - Chr8:27844926-27844945 None:intergenic 35.0%
GGAGAATGTAATGTAAAGAG+AGG - Chr8:27844235-27844254 None:intergenic 35.0%
GTGAAGAAAATGGTGAGTTT+AGG + Chr8:27844632-27844651 MsG0880043618.01.T01:CDS 35.0%
TAAGGCGGAGACTAATTTAA+TGG + Chr8:27846734-27846753 MsG0880043618.01.T01:intron 35.0%
TAAGTGGCCATGTATTCAAA+GGG + Chr8:27845626-27845645 MsG0880043618.01.T01:CDS 35.0%
TCATCATCACAGAATAATGC+TGG + Chr8:27844868-27844887 MsG0880043618.01.T01:CDS 35.0%
TCCAAGACAAATATGGTCAT+TGG - Chr8:27847405-27847424 None:intergenic 35.0%
TCCAATGACCATATTTGTCT+TGG + Chr8:27847401-27847420 MsG0880043618.01.T01:intron 35.0%
TGGTTAATAGAAAAATGCCC+CGG + Chr8:27845392-27845411 MsG0880043618.01.T01:intron 35.0%
TGTGTTTCAGAATTGCAACT+TGG + Chr8:27845694-27845713 MsG0880043618.01.T01:CDS 35.0%
TTGGCTACTGTGAATATAAG+TGG + Chr8:27845610-27845629 MsG0880043618.01.T01:CDS 35.0%
TTGTAGCTACCCTAATGTTT+AGG + Chr8:27847482-27847501 MsG0880043618.01.T01:intron 35.0%
! TGAAGATGAACTTGCTTACT+TGG + Chr8:27845591-27845610 MsG0880043618.01.T01:CDS 35.0%
! TGCTTGTTGAGATTGCTTTA+AGG + Chr8:27845795-27845814 MsG0880043618.01.T01:exon 35.0%
! TTCATCAAGACTCGAGTTTT+TGG + Chr8:27844503-27844522 MsG0880043618.01.T01:CDS 35.0%
! TTTAGGGTTTGTCAAGATTG+TGG + Chr8:27844649-27844668 MsG0880043618.01.T01:CDS 35.0%
!! AGTTGCAATTCTGAAACACA+AGG - Chr8:27845694-27845713 None:intergenic 35.0%
!!! GACACCTTTGTTGCATTTTT+TGG + Chr8:27846158-27846177 MsG0880043618.01.T01:intron 35.0%
!!! TGTTAGGGTTTTGATGATGA+GGG - Chr8:27844468-27844487 None:intergenic 35.0%
!!! TTGTTAGGGTTTTGATGATG+AGG - Chr8:27844469-27844488 None:intergenic 35.0%
AATCTCAACAAGCAGAAGCA+GGG - Chr8:27845789-27845808 None:intergenic 40.0%
ACTCGGGTCAAATTAACAGT+AGG + Chr8:27846584-27846603 MsG0880043618.01.T01:intron 40.0%
AGAATTCAAACCTGTAGCAG+TGG + Chr8:27844085-27844104 MsG0880043618.01.T01:five_prime_UTR 40.0%
ATATGTCAACAACTGACTGC+AGG - Chr8:27847270-27847289 None:intergenic 40.0%
CACATCATCAACTGTGTTAG+AGG + Chr8:27846361-27846380 MsG0880043618.01.T01:intron 40.0%
CCACTCTTTCCTTAGAAACT+AGG + Chr8:27847081-27847100 MsG0880043618.01.T01:intron 40.0%
GAGTTTCAGCAGTTACCAAA+TGG - Chr8:27847203-27847222 None:intergenic 40.0%
GATGAAGGTAAGGGAATGTT+TGG - Chr8:27844394-27844413 None:intergenic 40.0%
GGATGAGTCTGAAAATTGAC+TGG + Chr8:27847422-27847441 MsG0880043618.01.T01:intron 40.0%
GTAGGATTTCCATGCATAAC+CGG - Chr8:27847681-27847700 None:intergenic 40.0%
TATGTCAACAACTGACTGCA+GGG - Chr8:27847269-27847288 None:intergenic 40.0%
TCATCATCAGCACTAAGTGT+TGG + Chr8:27844409-27844428 MsG0880043618.01.T01:CDS 40.0%
TGTTTGTGAATTGCAGATCC+TGG + Chr8:27845490-27845509 MsG0880043618.01.T01:intron 40.0%
TTAATAGAAAAATGCCCCGG+AGG + Chr8:27845395-27845414 MsG0880043618.01.T01:intron 40.0%
TTCAACGATCACGACTATCT+TGG - Chr8:27847301-27847320 None:intergenic 40.0%
! ATAACTAGCTCTTGTCACCA+AGG + Chr8:27844952-27844971 MsG0880043618.01.T01:CDS 40.0%
! ATGATGGGGTGTGAAGAAAA+TGG + Chr8:27844622-27844641 MsG0880043618.01.T01:CDS 40.0%
! CAACTTGGAAGTAATGGAAG+TGG + Chr8:27845709-27845728 MsG0880043618.01.T01:CDS 40.0%
! CCTAGTTTCTAAGGAAAGAG+TGG - Chr8:27847084-27847103 None:intergenic 40.0%
! TGTAATCATAGGTGTCGTGT+CGG + Chr8:27845132-27845151 MsG0880043618.01.T01:intron 40.0%
! TGTTGGTTGTGAAGTGATGA+AGG - Chr8:27844343-27844362 None:intergenic 40.0%
!! ACACTTAGTGCTGATGATGA+AGG - Chr8:27844409-27844428 None:intergenic 40.0%
!! AGATGCAGGACTTGTTGTAA+AGG + Chr8:27844703-27844722 MsG0880043618.01.T01:CDS 40.0%
!! AGTGCTGATGATGAAGGTAA+GGG - Chr8:27844403-27844422 None:intergenic 40.0%
!! CTAACACAGTTGATGATGTG+TGG - Chr8:27846361-27846380 None:intergenic 40.0%
!! TAGTGCTGATGATGAAGGTA+AGG - Chr8:27844404-27844423 None:intergenic 40.0%
!! TTGCAACAAGGTTTTGGTGT+TGG - Chr8:27844274-27844293 None:intergenic 40.0%
!!! AGGGAGATTTTGGAACAGAA+AGG - Chr8:27844113-27844132 None:intergenic 40.0%
ATGGCAGGTAGTTCTTTCTC+CGG + Chr8:27847659-27847678 MsG0880043618.01.T01:intron 45.0%
CAATCTCAACAAGCAGAAGC+AGG - Chr8:27845790-27845809 None:intergenic 45.0%
CACGACACCTATGATTACAC+TGG - Chr8:27845131-27845150 None:intergenic 45.0%
CATGTTAGGCCTAAGAGACT+TGG + Chr8:27844300-27844319 MsG0880043618.01.T01:CDS 45.0%
CTATGGCACTTGAAAACAGC+AGG - Chr8:27845550-27845569 None:intergenic 45.0%
GTCAGAGAGGTAGACACAAT+TGG + Chr8:27845432-27845451 MsG0880043618.01.T01:intron 45.0%
GTTCTTTCTCCGGTTATGCA+TGG + Chr8:27847669-27847688 MsG0880043618.01.T01:three_prime_UTR 45.0%
TGCCAACAGCAGATACTCTA+TGG - Chr8:27845567-27845586 None:intergenic 45.0%
TGTCCGTGTTTCATAGGTCT+CGG - Chr8:27845047-27845066 None:intergenic 45.0%
! AGAGTATCTGCTGTTGGCAA+TGG + Chr8:27845568-27845587 MsG0880043618.01.T01:CDS 45.0%
! TGCCATAGAGTATCTGCTGT+TGG + Chr8:27845562-27845581 MsG0880043618.01.T01:CDS 45.0%
!! AGGACTTGTTGTAAAGGTCG+TGG + Chr8:27844709-27844728 MsG0880043618.01.T01:CDS 45.0%
!! ATTTAGTCTCAGAGGTGTCC+TGG - Chr8:27845190-27845209 None:intergenic 45.0%
!! TCAGCACTAAGTGTTGGTCT+TGG + Chr8:27844415-27844434 MsG0880043618.01.T01:CDS 45.0%
!! TTGATGATGAGGGTGAGTAG+TGG - Chr8:27844458-27844477 None:intergenic 45.0%
CGAACACGAACATACCGACA+CGG + Chr8:27845079-27845098 MsG0880043618.01.T01:intron 50.0%
CTTCAGCCACCATCATACTC+CGG - Chr8:27844792-27844811 None:intergenic 50.0%
GATGAGGGTGAGTAGTGGTT+AGG - Chr8:27844453-27844472 None:intergenic 50.0%
GGCAAAGTCTGCTTGAAACC+AGG - Chr8:27845511-27845530 None:intergenic 50.0%
GGTGAGTAGTGGTTAGGAGT+GGG - Chr8:27844447-27844466 None:intergenic 50.0%
GTCTGGTGTCCGTGTTTCAT+AGG - Chr8:27845053-27845072 None:intergenic 50.0%
GTGCTTCTTCTGGTACCAAG+AGG + Chr8:27844830-27844849 MsG0880043618.01.T01:CDS 50.0%
GTTGTGGTTGGCACATCTTG+AGG - Chr8:27844531-27844550 None:intergenic 50.0%
TGACTGCAGGGAATCCGATT+TGG - Chr8:27847257-27847276 None:intergenic 50.0%
TGGCCGAGACCTATGAAACA+CGG + Chr8:27845041-27845060 MsG0880043618.01.T01:intron 50.0%
! CATAGGTGTCGTGTCGGTAT+CGG + Chr8:27845138-27845157 MsG0880043618.01.T01:intron 50.0%
!! AGTATGATGGTGGCTGAAGG+TGG + Chr8:27844793-27844812 MsG0880043618.01.T01:CDS 50.0%
!! AGTGATGGTGGTGCTTCTTC+TGG + Chr8:27844820-27844839 MsG0880043618.01.T01:CDS 50.0%
AGAAAAATGCCCCGGAGGCA+TGG + Chr8:27845400-27845419 MsG0880043618.01.T01:intron 55.0%
ATGGTCGTCACTCGTCAGAG+AGG + Chr8:27845419-27845438 MsG0880043618.01.T01:intron 55.0%
GAAGCAGGGTAAGCACTAGC+TGG - Chr8:27845775-27845794 None:intergenic 55.0%
GAAGGTGGTGACAGTGATGG+TGG + Chr8:27844808-27844827 MsG0880043618.01.T01:CDS 55.0%
GCTGAAGGTGGTGACAGTGA+TGG + Chr8:27844805-27844824 MsG0880043618.01.T01:CDS 55.0%
GGGTGAGTAGTGGTTAGGAG+TGG - Chr8:27844448-27844467 None:intergenic 55.0%
GTTCGTGTTCGTGTCGTGTC+TGG - Chr8:27845070-27845089 None:intergenic 55.0%
! CGTGAAGAGCACGTGGATAC+CGG + Chr8:27844750-27844769 MsG0880043618.01.T01:CDS 55.0%
! GTACTCACGTGAAGAGCACG+TGG + Chr8:27844743-27844762 MsG0880043618.01.T01:CDS 55.0%
!! CAGAAAGGCACCACTGCTAC+AGG - Chr8:27844098-27844117 None:intergenic 55.0%
!! CGGAGTATGATGGTGGCTGA+AGG + Chr8:27844790-27844809 MsG0880043618.01.T01:CDS 55.0%
!! TCTCAGAGGTGTCCTGGTGT+CGG - Chr8:27845184-27845203 None:intergenic 55.0%
!! TGTCGTGTCGGTATCGGTGT+TGG + Chr8:27845144-27845163 MsG0880043618.01.T01:intron 55.0%
ACGAGTGACGACCATGCCTC+CGG - Chr8:27845414-27845433 None:intergenic 60.0%
ATCGACGTGTGTCCGACACC+AGG + Chr8:27845169-27845188 MsG0880043618.01.T01:intron 60.0%
CGAGTGACGACCATGCCTCC+GGG - Chr8:27845413-27845432 None:intergenic 65.0%
GAGGCGGGACCGGAGTATGA+TGG + Chr8:27844780-27844799 MsG0880043618.01.T01:CDS 65.0%
GAGTGACGACCATGCCTCCG+GGG - Chr8:27845412-27845431 None:intergenic 65.0%
GCGGGACCGGAGTATGATGG+TGG + Chr8:27844783-27844802 MsG0880043618.01.T01:CDS 65.0%
!! CGTGGATACCGGCGTCGATG+AGG + Chr8:27844761-27844780 MsG0880043618.01.T01:CDS 65.0%
!! GATACCGGCGTCGATGAGGC+GGG + Chr8:27844765-27844784 MsG0880043618.01.T01:CDS 65.0%
!! GGATACCGGCGTCGATGAGG+CGG + Chr8:27844764-27844783 MsG0880043618.01.T01:CDS 65.0%
GCAGGCGCGCGAACATAGCG+AGG - Chr8:27845532-27845551 None:intergenic 70.0%
CGGTCCCGCCTCATCGACGC+CGG - Chr8:27844772-27844791 None:intergenic 75.0%
!! CGGCGTCGATGAGGCGGGAC+CGG + Chr8:27844770-27844789 MsG0880043618.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 27844063 27847898 27844063 ID=MsG0880043618.01;Name=MsG0880043618.01
Chr8 mRNA 27844063 27847898 27844063 ID=MsG0880043618.01.T01;Parent=MsG0880043618.01;Name=MsG0880043618.01.T01;_AED=0.32;_eAED=0.32;_QI=232|1|1|1|0.5|0.33|3|245|325
Chr8 exon 27844063 27844973 27844063 ID=MsG0880043618.01.T01:exon:8826;Parent=MsG0880043618.01.T01
Chr8 exon 27845506 27845816 27845506 ID=MsG0880043618.01.T01:exon:8827;Parent=MsG0880043618.01.T01
Chr8 exon 27847666 27847898 27847666 ID=MsG0880043618.01.T01:exon:8828;Parent=MsG0880043618.01.T01
Chr8 five_prime_UTR 27844063 27844294 27844063 ID=MsG0880043618.01.T01:five_prime_utr;Parent=MsG0880043618.01.T01
Chr8 CDS 27844295 27844973 27844295 ID=MsG0880043618.01.T01:cds;Parent=MsG0880043618.01.T01
Chr8 CDS 27845506 27845804 27845506 ID=MsG0880043618.01.T01:cds;Parent=MsG0880043618.01.T01
Chr8 three_prime_UTR 27845805 27845816 27845805 ID=MsG0880043618.01.T01:three_prime_utr;Parent=MsG0880043618.01.T01
Chr8 three_prime_UTR 27847666 27847898 27847666 ID=MsG0880043618.01.T01:three_prime_utr;Parent=MsG0880043618.01.T01
Gene Sequence

>MsG0880043618.01.T01

ATGAACATGTTAGGCCTAAGAGACTTGGTTCTAATAGCTCAAACTCCTTCATCACTTCACAACCAACAAAACCAAACCATTTCATCAGATCATAATCCAAACATTCCCTTACCTTCATCATCAGCACTAAGTGTTGGTCTTGGAATTTTCCCACTCCTAACCACTACTCACCCTCATCATCAAAACCCTAACAATAACAATGAGAATATTCATCAAGACTCGAGTTTTTGGAACCTCAAGATGTGCCAACCACAACAAATAATGATGATGAATTCAGCAAGAAAAAGAGTTGAAGAAGAAGAAGATGATGTAAGAAACAAAAACTTGATGATGGGGTGTGAAGAAAATGGTGAGTTTAGGGTTTGTCAAGATTGTGGAAATAGAGCTAAGAAAGATTGTAGCTATAAAAGATGCAGGACTTGTTGTAAAGGTCGTGGTTTTGATTGTAGTACTCACGTGAAGAGCACGTGGATACCGGCGTCGATGAGGCGGGACCGGAGTATGATGGTGGCTGAAGGTGGTGACAGTGATGGTGGTGCTTCTTCTGGTACCAAGAGGCAGAGAATTTTGATTTCATCATCACAGAATAATGCTGGTGCTAACTCTCATAGTTCTAGTTCTAATGCTGCCACTAGAACAAGAAGTTTAAGCTTAGATATAACTAGCTCTTGTCACCAAGATCCTGGTTTCAAGCAGACTTTGCCTCGCTATGTTCGCGCGCCTGCTGTTTTCAAGTGCCATAGAGTATCTGCTGTTGGCAATGGTGAAGATGAACTTGCTTACTTGGCTACTGTGAATATAAGTGGCCATGTATTCAAAGGGTTTCTCTATGATCAAGGTGTTGATGACAAAAATGAAACAATGCCTTGTGTTTCAGAATTGCAACTTGGAAGTAATGGAAGTGGAAAGAGTAATAGAGAATGTTCTTCTTCTGCAATTGAGGTTCCAGCTAGTGCTTACCCTGCTTCTGCTTGTTGA
Protein sequence

>MsG0880043618.01.T01

MNMLGLRDLVLIAQTPSSLHNQQNQTISSDHNPNIPLPSSSALSVGLGIFPLLTTTHPHHQNPNNNNENIHQDSSFWNLKMCQPQQIMMMNSARKRVEEEEDDVRNKNLMMGCEENGEFRVCQDCGNRAKKDCSYKRCRTCCKGRGFDCSTHVKSTWIPASMRRDRSMMVAEGGDSDGGASSGTKRQRILISSSQNNAGANSHSSSSNAATRTRSLSLDITSSCHQDPGFKQTLPRYVRAPAVFKCHRVSAVGNGEDELAYLATVNISGHVFKGFLYDQGVDDKNETMPCVSELQLGSNGSGKSNRECSSSAIEVPASAYPASAC*