AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044869.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044869.01.T01 MTR_3g102370 79.814 322 27 3 1 284 1 322 1.58e-177 492
MsG0880044869.01.T01 MTR_1g094030 72.500 320 48 4 1 282 1 318 3.14e-157 441
MsG0880044869.01.T01 MTR_1g018760 71.385 325 52 5 1 284 1 325 4.89e-155 436
MsG0880044869.01.T01 MTR_4g109900 48.734 316 126 4 3 282 2 317 6.03e-87 263
MsG0880044869.01.T01 MTR_4g109920 47.500 320 125 5 3 282 2 318 4.91e-85 258
MsG0880044869.01.T01 MTR_8g031580 28.428 299 145 9 6 239 23 317 4.37e-17 80.5
MsG0880044869.01.T01 MTR_8g031580 28.428 299 145 9 6 239 23 317 7.82e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044869.01.T01 AT3G16150 69.538 325 58 4 1 284 1 325 4.75e-152 428
MsG0880044869.01.T01 AT5G08100 48.089 314 128 5 1 282 1 311 1.80e-85 258
MsG0880044869.01.T01 AT5G08100 44.444 234 96 4 80 282 1 231 9.93e-53 172

Find 82 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGCCACTTCACCCTTAT+TGG 0.146841 8:+50434992 None:intergenic
GTTCATGGAGGTTGGAATTT+TGG 0.152801 8:-50434922 MsG0880044869.01.T01:CDS
TCAATTGTAATGGAATGTTT+AGG 0.266625 8:-50434965 MsG0880044869.01.T01:CDS
AATAAGGGTGAAGTGGCTTA+TGG 0.296657 8:-50434990 MsG0880044869.01.T01:CDS
CAATTGTAATGGAATGTTTA+GGG 0.297709 8:-50434964 MsG0880044869.01.T01:CDS
GGTTGGGCCATTGCTGTTCA+TGG 0.306024 8:-50436275 MsG0880044869.01.T01:CDS
CGTCTTGATGAAGGGTTTGC+TGG 0.320904 8:-50435029 MsG0880044869.01.T01:CDS
TCTTATTGCTGTGTCCAATA+AGG 0.346186 8:-50435006 MsG0880044869.01.T01:CDS
TGTGCTGCTGCAACCTCAAC+TGG 0.368919 8:-50435254 MsG0880044869.01.T01:CDS
TGACGGTGTGTTGGAAGATT+AGG 0.377061 8:+50436236 None:intergenic
AGAAGATGTGGTGCTGTATC+TGG 0.390803 8:-50435791 MsG0880044869.01.T01:CDS
AGGGGAAGTGCAACTGAGAA+TGG 0.403815 8:-50434945 MsG0880044869.01.T01:CDS
AGCAAAAGGAACGGTGGAAA+TGG 0.416209 8:-50435840 MsG0880044869.01.T01:CDS
ACATTCGGTAAGAAGCTGTT+TGG 0.418583 8:+50436205 None:intergenic
GCTTATGGGTTCAATTGTAA+TGG 0.420402 8:-50434975 MsG0880044869.01.T01:CDS
GTTATGGAGTACAAAGACTA+TGG 0.421674 8:-50435086 MsG0880044869.01.T01:CDS
ATAAGGGTGAAGTGGCTTAT+GGG 0.422425 8:-50434989 MsG0880044869.01.T01:CDS
ATTGCTGTTCATGGCGGCGC+AGG 0.424510 8:-50436266 MsG0880044869.01.T01:CDS
CTTACCGAATGTCTCAATCT+TGG 0.425909 8:-50436194 MsG0880044869.01.T01:CDS
CATTCTTACCTCGCCTTCTC+CGG 0.431764 8:-50435711 MsG0880044869.01.T01:intron
ACAAGACGAGCAAGAGAGAT+TGG 0.433669 8:+50435750 None:intergenic
TGCAACTGAGAATGGGTTCA+TGG 0.453727 8:-50434937 MsG0880044869.01.T01:CDS
CTTATTGCTGTGTCCAATAA+GGG 0.458493 8:-50435005 MsG0880044869.01.T01:CDS
GGATCTGCTCTGACAGCAAA+AGG 0.463031 8:-50435854 MsG0880044869.01.T01:CDS
ATTAAGCATCGTCTTGATGA+AGG 0.463221 8:-50435038 MsG0880044869.01.T01:CDS
AACAAAATGAGTGGTCGAAT+CGG 0.464428 8:-50435221 MsG0880044869.01.T01:CDS
GACGTCGAGTGCAGAAGCAT+TGG 0.465190 8:+50436154 None:intergenic
ACCACACATCCGACGGTCTC+CGG 0.480495 8:+50435296 None:intergenic
TAATCTTCCAACACACCGTC+AGG 0.481716 8:-50436234 MsG0880044869.01.T01:CDS
CTCTCTTGCTCGTCTTGTCA+TGG 0.483008 8:-50435745 MsG0880044869.01.T01:CDS
CAAATCTTCTGCACCGGAGA+AGG 0.484878 8:+50435698 None:intergenic
ACGTGAAGTGGCTGCAGTTA+TGG 0.491646 8:-50435102 MsG0880044869.01.T01:CDS
GAGAATGGGTTCATGGAGGT+TGG 0.502391 8:-50434930 MsG0880044869.01.T01:CDS
ATCGGTGATTCACCTCTCAT+AGG 0.503828 8:-50435203 MsG0880044869.01.T01:CDS
GTTCATTAGCCCTCCAGTTG+AGG 0.507804 8:+50435241 None:intergenic
GATAGTCCAGTGAAAATGAA+TGG 0.507847 8:-50435341 MsG0880044869.01.T01:CDS
GATTCACCTCTCATAGGTGC+TGG 0.508182 8:-50435197 MsG0880044869.01.T01:CDS
GCTTCTTCCTGACGGTGTGT+TGG 0.511337 8:+50436227 None:intergenic
GTGTGTGGAGTTTCTTGCAC+TGG 0.514229 8:-50435158 MsG0880044869.01.T01:CDS
GGGGAAGTGCAACTGAGAAT+GGG 0.515436 8:-50434944 MsG0880044869.01.T01:CDS
TACAGCACCACATCTTCTCT+TGG 0.525365 8:+50435796 None:intergenic
GGAGTTTCTTGCACTGGTGA+AGG 0.536295 8:-50435152 MsG0880044869.01.T01:CDS
GGAAATGGAGGCTAGTATCA+TGG 0.537266 8:-50435825 MsG0880044869.01.T01:CDS
GATACCAAGATTGAGACATT+CGG 0.538052 8:+50436190 None:intergenic
GACCGTCGGATGTGTGGTGG+TGG 0.541481 8:-50435291 MsG0880044869.01.T01:CDS
GCTGTTTGGCTTCTTCCTGA+CGG 0.552502 8:+50436219 None:intergenic
TGGCACTCTTGCACGTGAAG+TGG 0.556232 8:-50435114 MsG0880044869.01.T01:CDS
GAGAAGGCGAGGTAAGAATG+AGG 0.562126 8:+50435714 None:intergenic
CCACGGCCAGAATTCAAAAG+TGG 0.564240 8:+50435875 None:intergenic
TGCGCCGCCATGAACAGCAA+TGG 0.567462 8:+50436268 None:intergenic
GTTTGATTATCGAATTCCAA+CGG 0.581762 8:-50435381 MsG0880044869.01.T01:CDS
GCCGGAGACCGTCGGATGTG+TGG 0.582295 8:-50435297 MsG0880044869.01.T01:CDS
GAAGGTGAAGCTATCATTCG+TGG 0.583136 8:-50435134 MsG0880044869.01.T01:CDS
CTATGGACTTCAACAAGCTG+TGG 0.585085 8:-50435069 MsG0880044869.01.T01:CDS
TGTTCATGGCGGCGCAGGTG+TGG 0.589949 8:-50436261 MsG0880044869.01.T01:CDS
GCCGATGAGCATTTACGCGC+CGG 0.592527 8:-50435315 MsG0880044869.01.T01:CDS
AGAAGGCGAGGTAAGAATGA+GGG 0.593655 8:+50435715 None:intergenic
TTAAGCATCGTCTTGATGAA+GGG 0.596066 8:-50435037 MsG0880044869.01.T01:CDS
AATTGTAATGGAATGTTTAG+GGG 0.599436 8:-50434963 MsG0880044869.01.T01:CDS
TTGCTGTCAGAGCAGATCCA+CGG 0.605965 8:+50435858 None:intergenic
GCTGCTGCAACCTCAACTGG+AGG 0.606206 8:-50435251 MsG0880044869.01.T01:CDS
TGCTCTGACAGCAAAAGGAA+CGG 0.607232 8:-50435849 MsG0880044869.01.T01:CDS
GTCCACCACCACACATCCGA+CGG 0.619811 8:+50435289 None:intergenic
AAAAGGAACGGTGGAAATGG+AGG 0.622339 8:-50435837 MsG0880044869.01.T01:CDS
CTGCTGCAACCTCAACTGGA+GGG 0.626492 8:-50435250 MsG0880044869.01.T01:CDS
GAAGGCGAGGTAAGAATGAG+GGG 0.630197 8:+50435716 None:intergenic
ATTTACGCGCCGGAGACCGT+CGG 0.633728 8:-50435305 MsG0880044869.01.T01:CDS
ATGGAGGCTAGTATCATGGA+TGG 0.635651 8:-50435821 MsG0880044869.01.T01:CDS
CACGGCCAGAATTCAAAAGT+GGG 0.635763 8:+50435876 None:intergenic
TCCGGCGCGTAAATGCTCAT+CGG 0.640097 8:+50435314 None:intergenic
CGAGTGCAGAAGCATTGGAT+CGG 0.648150 8:+50436159 None:intergenic
CTTCTGCACCGGAGAAGGCG+AGG 0.649661 8:+50435703 None:intergenic
TAAGTTCCAGCACCTATGAG+AGG 0.650295 8:+50435191 None:intergenic
TGTGTCCAATAAGGGTGAAG+TGG 0.655230 8:-50434997 MsG0880044869.01.T01:CDS
GGAGACCGTCGGATGTGTGG+TGG 0.674288 8:-50435294 MsG0880044869.01.T01:CDS
GATGGTACCAAGAGAAGATG+TGG 0.682088 8:-50435803 MsG0880044869.01.T01:CDS
TGGGCCATTGCTGTTCATGG+CGG 0.682587 8:-50436272 MsG0880044869.01.T01:CDS
ACTTATGCATGTGAAGTGTG+TGG 0.696667 8:-50435173 MsG0880044869.01.T01:CDS
GGGCTAATGAACAAAATGAG+TGG 0.699843 8:-50435230 MsG0880044869.01.T01:CDS
TCTGACAGCAAAAGGAACGG+TGG 0.711989 8:-50435846 MsG0880044869.01.T01:CDS
TGTGTGGTGGTGGACAGTGA+AGG 0.729603 8:-50435281 MsG0880044869.01.T01:CDS
AACTGAGAATGGGTTCATGG+AGG 0.788867 8:-50434934 MsG0880044869.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTTATAGATCAAAAA+TGG + Chr8:50435563-50435582 None:intergenic 10.0%
!! ATTTATATCATTAATAATCA+TGG - Chr8:50435126-50435145 MsG0880044869.01.T01:CDS 10.0%
!! GATTATTAATGATATAAATT+AGG + Chr8:50435125-50435144 None:intergenic 10.0%
!! TTTATATCATTAATAATCAT+GGG - Chr8:50435127-50435146 MsG0880044869.01.T01:CDS 10.0%
!!! CAATAATATATTTTTAAATC+GGG - Chr8:50435216-50435235 MsG0880044869.01.T01:CDS 10.0%
!! TTAATGATATAAATTAGGTT+AGG + Chr8:50435120-50435139 None:intergenic 15.0%
!!! AATTAGGTTAGGTTTTATTA+CGG + Chr8:50435109-50435128 None:intergenic 20.0%
!!! TTTCTTTTTCAACAGATATT+TGG - Chr8:50435582-50435601 MsG0880044869.01.T01:intron 20.0%
! AAAGGAAGCTAATACAATAT+TGG - Chr8:50435684-50435703 MsG0880044869.01.T01:intron 25.0%
! AACCCTAAAATCAACAAAAA+CGG + Chr8:50435269-50435288 None:intergenic 25.0%
! TCACTTGTGTAATTAAAATG+TGG - Chr8:50435743-50435762 MsG0880044869.01.T01:CDS 25.0%
! TCATTAATAATCATGGGATA+AGG - Chr8:50435133-50435152 MsG0880044869.01.T01:CDS 25.0%
! TTACTAATGAATGTGTTTGA+TGG - Chr8:50435783-50435802 MsG0880044869.01.T01:CDS 25.0%
!! AATTGTAATGGAATGTTTAG+GGG - Chr8:50436235-50436254 MsG0880044869.01.T01:CDS 25.0%
!! CAATTGTAATGGAATGTTTA+GGG - Chr8:50436234-50436253 MsG0880044869.01.T01:CDS 25.0%
!! TCAATTGTAATGGAATGTTT+AGG - Chr8:50436233-50436252 MsG0880044869.01.T01:CDS 25.0%
!! TTTTATTACGGAAAAGACAT+GGG + Chr8:50435097-50435116 None:intergenic 25.0%
!!! ATTTGGTCATGTTTTTTCTA+GGG - Chr8:50435599-50435618 MsG0880044869.01.T01:intron 25.0%
!!! TATTTGGTCATGTTTTTTCT+AGG - Chr8:50435598-50435617 MsG0880044869.01.T01:intron 25.0%
!!! TTCCGTTTTTGTTGATTTTA+GGG - Chr8:50435264-50435283 MsG0880044869.01.T01:CDS 25.0%
!!! TTTCCGTTTTTGTTGATTTT+AGG - Chr8:50435263-50435282 MsG0880044869.01.T01:CDS 25.0%
GTTTGATTATCGAATTCCAA+CGG - Chr8:50435817-50435836 MsG0880044869.01.T01:CDS 30.0%
TAATGAATGTGTTTGATGGA+CGG - Chr8:50435787-50435806 MsG0880044869.01.T01:CDS 30.0%
TTTCAGCATACCAACATTAT+CGG + Chr8:50435659-50435678 None:intergenic 30.0%
!! GTTTTATTACGGAAAAGACA+TGG + Chr8:50435098-50435117 None:intergenic 30.0%
!! TTTAGGGTTCTTTTTGTGAT+AGG - Chr8:50435280-50435299 MsG0880044869.01.T01:CDS 30.0%
AACAAAATGAGTGGTCGAAT+CGG - Chr8:50435977-50435996 MsG0880044869.01.T01:intron 35.0%
CTTATTGCTGTGTCCAATAA+GGG - Chr8:50436193-50436212 MsG0880044869.01.T01:CDS 35.0%
GATACCAAGATTGAGACATT+CGG + Chr8:50435011-50435030 None:intergenic 35.0%
GATAGTCCAGTGAAAATGAA+TGG - Chr8:50435857-50435876 MsG0880044869.01.T01:CDS 35.0%
GCTTATGGGTTCAATTGTAA+TGG - Chr8:50436223-50436242 MsG0880044869.01.T01:CDS 35.0%
GTTATGGAGTACAAAGACTA+TGG - Chr8:50436112-50436131 MsG0880044869.01.T01:intron 35.0%
TCTTATTGCTGTGTCCAATA+AGG - Chr8:50436192-50436211 MsG0880044869.01.T01:CDS 35.0%
TTCAGCATACCAACATTATC+GGG + Chr8:50435658-50435677 None:intergenic 35.0%
! ATTAAGCATCGTCTTGATGA+AGG - Chr8:50436160-50436179 MsG0880044869.01.T01:CDS 35.0%
! CAAGAGAGATTGGATTTTCA+CGG + Chr8:50435441-50435460 None:intergenic 35.0%
! TTAAGCATCGTCTTGATGAA+GGG - Chr8:50436161-50436180 MsG0880044869.01.T01:CDS 35.0%
AATAAGGGTGAAGTGGCTTA+TGG - Chr8:50436208-50436227 MsG0880044869.01.T01:CDS 40.0%
ACATTCGGTAAGAAGCTGTT+TGG + Chr8:50434996-50435015 None:intergenic 40.0%
ACTTATGCATGTGAAGTGTG+TGG - Chr8:50436025-50436044 MsG0880044869.01.T01:intron 40.0%
ATAAGCCACTTCACCCTTAT+TGG + Chr8:50436209-50436228 None:intergenic 40.0%
ATAAGGGTGAAGTGGCTTAT+GGG - Chr8:50436209-50436228 MsG0880044869.01.T01:CDS 40.0%
ATTCCAACGGAAAAATGTGC+TGG - Chr8:50435830-50435849 MsG0880044869.01.T01:CDS 40.0%
CTTACCGAATGTCTCAATCT+TGG - Chr8:50435004-50435023 MsG0880044869.01.T01:CDS 40.0%
GGGCTAATGAACAAAATGAG+TGG - Chr8:50435968-50435987 MsG0880044869.01.T01:intron 40.0%
TTCATCACTCCCGATAATGT+TGG - Chr8:50435646-50435665 MsG0880044869.01.T01:intron 40.0%
! GTTCATGGAGGTTGGAATTT+TGG - Chr8:50436276-50436295 MsG0880044869.01.T01:CDS 40.0%
!! ACAGACCCACTTTTGAATTC+TGG - Chr8:50435317-50435336 MsG0880044869.01.T01:CDS 40.0%
AAAAGGAACGGTGGAAATGG+AGG - Chr8:50435361-50435380 MsG0880044869.01.T01:CDS 45.0%
ACAAGACGAGCAAGAGAGAT+TGG + Chr8:50435451-50435470 None:intergenic 45.0%
AGAAGGCGAGGTAAGAATGA+GGG + Chr8:50435486-50435505 None:intergenic 45.0%
AGCAAAAGGAACGGTGGAAA+TGG - Chr8:50435358-50435377 MsG0880044869.01.T01:CDS 45.0%
ATCGGTGATTCACCTCTCAT+AGG - Chr8:50435995-50436014 MsG0880044869.01.T01:intron 45.0%
ATGGAGGCTAGTATCATGGA+TGG - Chr8:50435377-50435396 MsG0880044869.01.T01:CDS 45.0%
CACGGCCAGAATTCAAAAGT+GGG + Chr8:50435325-50435344 None:intergenic 45.0%
CTATGGACTTCAACAAGCTG+TGG - Chr8:50436129-50436148 MsG0880044869.01.T01:intron 45.0%
GAAGGTGAAGCTATCATTCG+TGG - Chr8:50436064-50436083 MsG0880044869.01.T01:intron 45.0%
GATGGTACCAAGAGAAGATG+TGG - Chr8:50435395-50435414 MsG0880044869.01.T01:intron 45.0%
GGAAATGGAGGCTAGTATCA+TGG - Chr8:50435373-50435392 MsG0880044869.01.T01:CDS 45.0%
TAAGTTCCAGCACCTATGAG+AGG + Chr8:50436010-50436029 None:intergenic 45.0%
TAATCTTCCAACACACCGTC+AGG - Chr8:50434964-50434983 MsG0880044869.01.T01:CDS 45.0%
TACAGCACCACATCTTCTCT+TGG + Chr8:50435405-50435424 None:intergenic 45.0%
TGACGGTGTGTTGGAAGATT+AGG + Chr8:50434965-50434984 None:intergenic 45.0%
TGCTCTGACAGCAAAAGGAA+CGG - Chr8:50435349-50435368 MsG0880044869.01.T01:CDS 45.0%
TGGTATGCTGAAACTCGCAA+AGG - Chr8:50435666-50435685 MsG0880044869.01.T01:intron 45.0%
TGTGTCCAATAAGGGTGAAG+TGG - Chr8:50436201-50436220 MsG0880044869.01.T01:CDS 45.0%
! AACTGAGAATGGGTTCATGG+AGG - Chr8:50436264-50436283 MsG0880044869.01.T01:CDS 45.0%
! GAGAGATTGGATTTTCACGG+TGG + Chr8:50435438-50435457 None:intergenic 45.0%
! GGCAGACCATTCATTTTCAC+TGG + Chr8:50435866-50435885 None:intergenic 45.0%
! GTTCCAGCACATTTTTCCGT+TGG + Chr8:50435836-50435855 None:intergenic 45.0%
! TGAATGTGTTTGATGGACGG+TGG - Chr8:50435790-50435809 MsG0880044869.01.T01:CDS 45.0%
! TGCAACTGAGAATGGGTTCA+TGG - Chr8:50436261-50436280 MsG0880044869.01.T01:CDS 45.0%
!! AGAAGATGTGGTGCTGTATC+TGG - Chr8:50435407-50435426 MsG0880044869.01.T01:intron 45.0%
!! CAGAAGATTTGCTAGGGAAC+AGG - Chr8:50435512-50435531 MsG0880044869.01.T01:intron 45.0%
!!! GTCATGTTTTTTCTAGGGCG+TGG - Chr8:50435604-50435623 MsG0880044869.01.T01:intron 45.0%
!!! AAAATATATTATTGTTATTA+TGG + Chr8:50435210-50435229 None:intergenic 5.0%
!!! ACAATAATATATTTTTAAAT+CGG - Chr8:50435215-50435234 MsG0880044869.01.T01:CDS 5.0%
ACGTGAAGTGGCTGCAGTTA+TGG - Chr8:50436096-50436115 MsG0880044869.01.T01:intron 50.0%
CAAATCTTCTGCACCGGAGA+AGG + Chr8:50435503-50435522 None:intergenic 50.0%
CATTCTTACCTCGCCTTCTC+CGG - Chr8:50435487-50435506 MsG0880044869.01.T01:intron 50.0%
CCACGGCCAGAATTCAAAAG+TGG + Chr8:50435326-50435345 None:intergenic 50.0%
CCCTAGCAAATCTTCTGCAC+CGG + Chr8:50435509-50435528 None:intergenic 50.0%
CGTCTTGATGAAGGGTTTGC+TGG - Chr8:50436169-50436188 MsG0880044869.01.T01:CDS 50.0%
CTCTCTTGCTCGTCTTGTCA+TGG - Chr8:50435453-50435472 MsG0880044869.01.T01:intron 50.0%
GAAGGCGAGGTAAGAATGAG+GGG + Chr8:50435485-50435504 None:intergenic 50.0%
GAGAAGGCGAGGTAAGAATG+AGG + Chr8:50435487-50435506 None:intergenic 50.0%
GATTCACCTCTCATAGGTGC+TGG - Chr8:50436001-50436020 MsG0880044869.01.T01:intron 50.0%
GGATCTGCTCTGACAGCAAA+AGG - Chr8:50435344-50435363 MsG0880044869.01.T01:CDS 50.0%
GTGTGTGGAGTTTCTTGCAC+TGG - Chr8:50436040-50436059 MsG0880044869.01.T01:intron 50.0%
GTTCATTAGCCCTCCAGTTG+AGG + Chr8:50435960-50435979 None:intergenic 50.0%
TCTGACAGCAAAAGGAACGG+TGG - Chr8:50435352-50435371 MsG0880044869.01.T01:CDS 50.0%
TTGCTGTCAGAGCAGATCCA+CGG + Chr8:50435343-50435362 None:intergenic 50.0%
! AGGGGAAGTGCAACTGAGAA+TGG - Chr8:50436253-50436272 MsG0880044869.01.T01:CDS 50.0%
! GAGAATGGGTTCATGGAGGT+TGG - Chr8:50436268-50436287 MsG0880044869.01.T01:CDS 50.0%
! GCTGTTTGGCTTCTTCCTGA+CGG + Chr8:50434982-50435001 None:intergenic 50.0%
! GGAGTTTCTTGCACTGGTGA+AGG - Chr8:50436046-50436065 MsG0880044869.01.T01:intron 50.0%
! GGGGAAGTGCAACTGAGAAT+GGG - Chr8:50436254-50436273 MsG0880044869.01.T01:CDS 50.0%
! TCCGGTGCAGAAGATTTGCT+AGG - Chr8:50435505-50435524 MsG0880044869.01.T01:intron 50.0%
!! CCACTTTTGAATTCTGGCCG+TGG - Chr8:50435323-50435342 MsG0880044869.01.T01:CDS 50.0%
!! CCGGTGCAGAAGATTTGCTA+GGG - Chr8:50435506-50435525 MsG0880044869.01.T01:intron 50.0%
!! CGAGTGCAGAAGCATTGGAT+CGG + Chr8:50435042-50435061 None:intergenic 50.0%
CTGCTGCAACCTCAACTGGA+GGG - Chr8:50435948-50435967 MsG0880044869.01.T01:intron 55.0%
GCTTCTTCCTGACGGTGTGT+TGG + Chr8:50434974-50434993 None:intergenic 55.0%
TCCGGCGCGTAAATGCTCAT+CGG + Chr8:50435887-50435906 None:intergenic 55.0%
TGGCACTCTTGCACGTGAAG+TGG - Chr8:50436084-50436103 MsG0880044869.01.T01:intron 55.0%
! GACGTCGAGTGCAGAAGCAT+TGG + Chr8:50435047-50435066 None:intergenic 55.0%
! GGTTGGGCCATTGCTGTTCA+TGG - Chr8:50434923-50434942 MsG0880044869.01.T01:CDS 55.0%
! TGGGCCATTGCTGTTCATGG+CGG - Chr8:50434926-50434945 MsG0880044869.01.T01:CDS 55.0%
! TGTGCTGCTGCAACCTCAAC+TGG - Chr8:50435944-50435963 MsG0880044869.01.T01:intron 55.0%
!! TGTGTGGTGGTGGACAGTGA+AGG - Chr8:50435917-50435936 MsG0880044869.01.T01:intron 55.0%
!! TTTCTAGGGCGTGGAGACTG+AGG - Chr8:50435613-50435632 MsG0880044869.01.T01:intron 55.0%
ACCACACATCCGACGGTCTC+CGG + Chr8:50435905-50435924 None:intergenic 60.0%
ATTTACGCGCCGGAGACCGT+CGG - Chr8:50435893-50435912 MsG0880044869.01.T01:CDS 60.0%
GCCGATGAGCATTTACGCGC+CGG - Chr8:50435883-50435902 MsG0880044869.01.T01:CDS 60.0%
GCTGCTGCAACCTCAACTGG+AGG - Chr8:50435947-50435966 MsG0880044869.01.T01:intron 60.0%
GTCCACCACCACACATCCGA+CGG + Chr8:50435912-50435931 None:intergenic 60.0%
TGCGCCGCCATGAACAGCAA+TGG + Chr8:50434933-50434952 None:intergenic 60.0%
! ATTGCTGTTCATGGCGGCGC+AGG - Chr8:50434932-50434951 MsG0880044869.01.T01:CDS 60.0%
CTTCTGCACCGGAGAAGGCG+AGG + Chr8:50435498-50435517 None:intergenic 65.0%
GACCGTCGGATGTGTGGTGG+TGG - Chr8:50435907-50435926 MsG0880044869.01.T01:intron 65.0%
GGAGACCGTCGGATGTGTGG+TGG - Chr8:50435904-50435923 MsG0880044869.01.T01:intron 65.0%
TGTTCATGGCGGCGCAGGTG+TGG - Chr8:50434937-50434956 MsG0880044869.01.T01:CDS 65.0%
GCCGGAGACCGTCGGATGTG+TGG - Chr8:50435901-50435920 MsG0880044869.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 50434917 50436303 50434917 ID=MsG0880044869.01;Name=MsG0880044869.01
Chr8 mRNA 50434917 50436303 50434917 ID=MsG0880044869.01.T01;Parent=MsG0880044869.01;Name=MsG0880044869.01.T01;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|284
Chr8 exon 50436142 50436303 50436142 ID=MsG0880044869.01.T01:exon:21355;Parent=MsG0880044869.01.T01
Chr8 exon 50435712 50435918 50435712 ID=MsG0880044869.01.T01:exon:21354;Parent=MsG0880044869.01.T01
Chr8 exon 50434917 50435402 50434917 ID=MsG0880044869.01.T01:exon:21353;Parent=MsG0880044869.01.T01
Chr8 CDS 50436142 50436303 50436142 ID=MsG0880044869.01.T01:cds;Parent=MsG0880044869.01.T01
Chr8 CDS 50435712 50435918 50435712 ID=MsG0880044869.01.T01:cds;Parent=MsG0880044869.01.T01
Chr8 CDS 50434917 50435402 50434917 ID=MsG0880044869.01.T01:cds;Parent=MsG0880044869.01.T01
Gene Sequence

>MsG0880044869.01.T01

ATGTCTGGTTGGGCCATTGCTGTTCATGGCGGCGCAGGTGTGGATCCTAATCTTCCAACACACCGTCAGGAAGAAGCCAAACAGCTTCTTACCGAATGTCTCAATCTTGGTATCTCTGCTCTCCGATCCAATGCTTCTGCACTCGACGTCGTTGAACTCGTCGTGAGAAAGTTAGAAACAGACCCACTTTTGAATTCTGGCCGTGGATCTGCTCTGACAGCAAAAGGAACGGTGGAAATGGAGGCTAGTATCATGGATGGTACCAAGAGAAGATGTGGTGCTGTATCTGGTGTTTCCACCGTGAAAATCCAATCTCTCTTGCTCGTCTTGTCATGGAAAAATCCCCTCATTCTTACCTCGCCTTCTCCGTTTGATTATCGAATTCCAACGGAAAAATGTGCTGGAACTGATAGTCCAGTGAAAATGAATGGTCTGCCGATGAGCATTTACGCGCCGGAGACCGTCGGATGTGTGGTGGTGGACAGTGAAGGAAGATGTGCTGCTGCAACCTCAACTGGAGGGCTAATGAACAAAATGAGTGGTCGAATCGGTGATTCACCTCTCATAGGTGCTGGAACTTATGCATGTGAAGTGTGTGGAGTTTCTTGCACTGGTGAAGGTGAAGCTATCATTCGTGGCACTCTTGCACGTGAAGTGGCTGCAGTTATGGAGTACAAAGACTATGGACTTCAACAAGCTGTGGATTTTGTGATTAAGCATCGTCTTGATGAAGGGTTTGCTGGTCTTATTGCTGTGTCCAATAAGGGTGAAGTGGCTTATGGGTTCAATTGTAATGGAATGTTTAGGGGAAGTGCAACTGAGAATGGGTTCATGGAGGTTGGAATTTTGGAATGA
Protein sequence

>MsG0880044869.01.T01

MSGWAIAVHGGAGVDPNLPTHRQEEAKQLLTECLNLGISALRSNASALDVVELVVRKLETDPLLNSGRGSALTAKGTVEMEASIMDGTKRRCGAVSGVSTVKIQSLLLVLSWKNPLILTSPSPFDYRIPTEKCAGTDSPVKMNGLPMSIYAPETVGCVVVDSEGRCAAATSTGGLMNKMSGRIGDSPLIGAGTYACEVCGVSCTGEGEAIIRGTLAREVAAVMEYKDYGLQQAVDFVIKHRLDEGFAGLIAVSNKGEVAYGFNCNGMFRGSATENGFMEVGILE*