Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044940.01.T01 | PNY01313.1 | 90.805 | 87 | 8 | 0 | 2 | 88 | 46 | 132 | 2.26E-49 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044940.01.T01 | P93736 | 79.31 | 87 | 18 | 0 | 2 | 88 | 595 | 681 | 2.86E-44 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044940.01.T01 | A0A2K3NE61 | 90.805 | 87 | 8 | 0 | 2 | 88 | 46 | 132 | 1.08e-49 | 174 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380014676.01 | MsG0880044940.01 | 0.808773 | 2.627138e-50 | 1.515241e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044940.01.T01 | MTR_1g101620 | 95.402 | 87 | 4 | 0 | 2 | 88 | 557 | 643 | 9.26e-53 | 177 |
| MsG0880044940.01.T01 | MTR_6g027620 | 58.824 | 85 | 33 | 2 | 4 | 87 | 505 | 588 | 9.44e-29 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044940.01.T01 | AT1G14610 | 79.310 | 87 | 18 | 0 | 2 | 88 | 595 | 681 | 2.92e-45 | 156 |
| MsG0880044940.01.T01 | AT5G16715 | 58.140 | 86 | 34 | 2 | 3 | 87 | 499 | 583 | 6.30e-27 | 103 |
Find 29 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTTCCCTTTGACTGTAC+TGG | 0.303531 | 8:+51786739 | MsG0880044940.01.T01:CDS |
| GTAACCTCACCTTTGCTAAA+AGG | 0.314695 | 8:-51786890 | None:intergenic |
| GAAAGAAGTTTCATTTAATC+CGG | 0.328511 | 8:+51786681 | MsG0880044940.01.T01:CDS |
| ACTTCTTTCCATTATACCTT+TGG | 0.358329 | 8:-51786667 | None:intergenic |
| AATCCGGGACCCTGATGTTC+TGG | 0.363362 | 8:+51786697 | MsG0880044940.01.T01:CDS |
| GAGGATAACCAAAGGTATAA+TGG | 0.365652 | 8:+51786659 | MsG0880044940.01.T01:CDS |
| AACCATGTATCCAGAACATC+AGG | 0.382472 | 8:-51786707 | None:intergenic |
| CTGTTCCCTTTGACTGTACT+GGG | 0.401639 | 8:+51786740 | MsG0880044940.01.T01:CDS |
| GTTGCTCGCATGGTTATGTT+AGG | 0.404144 | 8:+51786845 | MsG0880044940.01.T01:CDS |
| CCTTTGGTTATCCTCATCTC+TGG | 0.412856 | 8:-51786651 | None:intergenic |
| GGTTATGTTAGGAATGAAGT+TGG | 0.418816 | 8:+51786856 | MsG0880044940.01.T01:CDS |
| CTCAAGTCGTCTGTGTCATC+AGG | 0.421978 | 8:-51786767 | None:intergenic |
| CCAACTTCTGTATTGGAAAC+CGG | 0.440081 | 8:+51786800 | MsG0880044940.01.T01:CDS |
| TTACTATCTTTCTGATACTC+AGG | 0.441960 | 8:+51786909 | MsG0880044940.01.T01:CDS |
| CCGGTTTCCAATACAGAAGT+TGG | 0.444304 | 8:-51786800 | None:intergenic |
| ACCCTGATGTTCTGGATACA+TGG | 0.447832 | 8:+51786705 | MsG0880044940.01.T01:CDS |
| GTTATGTTAGGAATGAAGTT+GGG | 0.455590 | 8:+51786857 | MsG0880044940.01.T01:CDS |
| GGCCATCCCAGTACAGTCAA+AGG | 0.513909 | 8:-51786746 | None:intergenic |
| AAAGAAGTTTCATTTAATCC+GGG | 0.515836 | 8:+51786682 | MsG0880044940.01.T01:CDS |
| TCCCTTTGACTGTACTGGGA+TGG | 0.518559 | 8:+51786744 | MsG0880044940.01.T01:CDS |
| TGATGACACAGACGACTTGA+GGG | 0.543174 | 8:+51786769 | MsG0880044940.01.T01:CDS |
| CTGATGACACAGACGACTTG+AGG | 0.551100 | 8:+51786768 | MsG0880044940.01.T01:CDS |
| TATCCAGAACATCAGGGTCC+CGG | 0.552895 | 8:-51786700 | None:intergenic |
| GCCATCCCAGTACAGTCAAA+GGG | 0.554762 | 8:-51786745 | None:intergenic |
| ATGTTAGGAATGAAGTTGGG+AGG | 0.591498 | 8:+51786860 | MsG0880044940.01.T01:CDS |
| GAAGAAAGAGGCCAGAGATG+AGG | 0.602181 | 8:+51786640 | MsG0880044940.01.T01:CDS |
| CTATCTTTCTGATACTCAGG+TGG | 0.629994 | 8:+51786912 | MsG0880044940.01.T01:CDS |
| CCAGAGATGAGGATAACCAA+AGG | 0.693988 | 8:+51786651 | MsG0880044940.01.T01:CDS |
| ACCATGTATCCAGAACATCA+GGG | 0.754192 | 8:-51786706 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAGTTTCATTTAATCC+GGG | + | Chr8:51786682-51786701 | MsG0880044940.01.T01:CDS | 25.0% |
| ! | GAAAGAAGTTTCATTTAATC+CGG | + | Chr8:51786681-51786700 | MsG0880044940.01.T01:CDS | 25.0% |
| !! | ATTTTATCCAACTTCTGTAT+TGG | + | Chr8:51786793-51786812 | MsG0880044940.01.T01:CDS | 25.0% |
| ACTTCTTTCCATTATACCTT+TGG | - | Chr8:51786670-51786689 | None:intergenic | 30.0% | |
| GTTATGTTAGGAATGAAGTT+GGG | + | Chr8:51786857-51786876 | MsG0880044940.01.T01:CDS | 30.0% | |
| TTACTATCTTTCTGATACTC+AGG | + | Chr8:51786909-51786928 | MsG0880044940.01.T01:CDS | 30.0% | |
| GGTTATGTTAGGAATGAAGT+TGG | + | Chr8:51786856-51786875 | MsG0880044940.01.T01:CDS | 35.0% | |
| ! | GAGGATAACCAAAGGTATAA+TGG | + | Chr8:51786659-51786678 | MsG0880044940.01.T01:CDS | 35.0% |
| AACCATGTATCCAGAACATC+AGG | - | Chr8:51786710-51786729 | None:intergenic | 40.0% | |
| ACCATGTATCCAGAACATCA+GGG | - | Chr8:51786709-51786728 | None:intergenic | 40.0% | |
| ATGTTAGGAATGAAGTTGGG+AGG | + | Chr8:51786860-51786879 | MsG0880044940.01.T01:CDS | 40.0% | |
| CCAACTTCTGTATTGGAAAC+CGG | + | Chr8:51786800-51786819 | MsG0880044940.01.T01:CDS | 40.0% | |
| CCCAGAAAAAGAGGATATCA+CGG | - | Chr8:51786826-51786845 | None:intergenic | 40.0% | |
| CTATCTTTCTGATACTCAGG+TGG | + | Chr8:51786912-51786931 | MsG0880044940.01.T01:CDS | 40.0% | |
| GTAACCTCACCTTTGCTAAA+AGG | - | Chr8:51786893-51786912 | None:intergenic | 40.0% | |
| ! | CCGTGATATCCTCTTTTTCT+GGG | + | Chr8:51786823-51786842 | MsG0880044940.01.T01:CDS | 40.0% |
| ! | CTGGATACATGGTTTTCTTC+TGG | + | Chr8:51786716-51786735 | MsG0880044940.01.T01:CDS | 40.0% |
| CCAGAGATGAGGATAACCAA+AGG | + | Chr8:51786651-51786670 | MsG0880044940.01.T01:CDS | 45.0% | |
| CCGGTTTCCAATACAGAAGT+TGG | - | Chr8:51786803-51786822 | None:intergenic | 45.0% | |
| CCTTTGGTTATCCTCATCTC+TGG | - | Chr8:51786654-51786673 | None:intergenic | 45.0% | |
| CTGTTCCCTTTGACTGTACT+GGG | + | Chr8:51786740-51786759 | MsG0880044940.01.T01:CDS | 45.0% | |
| GAAAAAGAGGATATCACGGC+CGG | - | Chr8:51786822-51786841 | None:intergenic | 45.0% | |
| GTTGCTCGCATGGTTATGTT+AGG | + | Chr8:51786845-51786864 | MsG0880044940.01.T01:CDS | 45.0% | |
| TCTGTTCCCTTTGACTGTAC+TGG | + | Chr8:51786739-51786758 | MsG0880044940.01.T01:CDS | 45.0% | |
| TGATGACACAGACGACTTGA+GGG | + | Chr8:51786769-51786788 | MsG0880044940.01.T01:CDS | 45.0% | |
| ! | ACCCTGATGTTCTGGATACA+TGG | + | Chr8:51786705-51786724 | MsG0880044940.01.T01:CDS | 45.0% |
| ! | GCCGTGATATCCTCTTTTTC+TGG | + | Chr8:51786822-51786841 | MsG0880044940.01.T01:CDS | 45.0% |
| ! | GGTGATGTGCCTTTTAGCAA+AGG | + | Chr8:51786881-51786900 | MsG0880044940.01.T01:CDS | 45.0% |
| ! | TGTGCCTTTTAGCAAAGGTG+AGG | + | Chr8:51786886-51786905 | MsG0880044940.01.T01:CDS | 45.0% |
| CTCAAGTCGTCTGTGTCATC+AGG | - | Chr8:51786770-51786789 | None:intergenic | 50.0% | |
| CTGATGACACAGACGACTTG+AGG | + | Chr8:51786768-51786787 | MsG0880044940.01.T01:CDS | 50.0% | |
| GAAGAAAGAGGCCAGAGATG+AGG | + | Chr8:51786640-51786659 | MsG0880044940.01.T01:CDS | 50.0% | |
| GCCATCCCAGTACAGTCAAA+GGG | - | Chr8:51786748-51786767 | None:intergenic | 50.0% | |
| TATCCAGAACATCAGGGTCC+CGG | - | Chr8:51786703-51786722 | None:intergenic | 50.0% | |
| TCCCTTTGACTGTACTGGGA+TGG | + | Chr8:51786744-51786763 | MsG0880044940.01.T01:CDS | 50.0% | |
| TGCGAGCAACCCAGAAAAAG+AGG | - | Chr8:51786835-51786854 | None:intergenic | 50.0% | |
| ! | CTTTTTCTGGGTTGCTCGCA+TGG | + | Chr8:51786835-51786854 | MsG0880044940.01.T01:CDS | 50.0% |
| AATCCGGGACCCTGATGTTC+TGG | + | Chr8:51786697-51786716 | MsG0880044940.01.T01:CDS | 55.0% | |
| GGCCATCCCAGTACAGTCAA+AGG | - | Chr8:51786749-51786768 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 51786638 | 51786970 | 51786638 | ID=MsG0880044940.01;Name=MsG0880044940.01 |
| Chr8 | mRNA | 51786638 | 51786970 | 51786638 | ID=MsG0880044940.01.T01;Parent=MsG0880044940.01;Name=MsG0880044940.01.T01;_AED=0.58;_eAED=0.58;_QI=0|-1|0|1|-1|1|1|0|110 |
| Chr8 | exon | 51786638 | 51786970 | 51786638 | ID=MsG0880044940.01.T01:exon:29147;Parent=MsG0880044940.01.T01 |
| Chr8 | CDS | 51786638 | 51786970 | 51786638 | ID=MsG0880044940.01.T01:cds;Parent=MsG0880044940.01.T01 |
| Gene Sequence |
| Protein sequence |
