AlfalfaGEDB | MsG0880044960.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044960.01

KEGG: -
GO: GO:0016021 GO:0055085
PFAM: PF07690

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044960.01.T01	XP_003628644.2	80.59	407	28	5	5	362	47	451	0	632

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044960.01.T01	Q3EAQ5	50.242	414	146	7	5	364	45	452	2.39E-129	382

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044960.01.T01	G7LFE1	80.590	407	28	5	5	362	47	451	0.0	632

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0880044960.01	MsG0880043870.01	PPI
MsG0880044960.01	MsG0380011924.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044960.01.T01	MTR_8g062990	80.590	407	28	5	5	362	47	451	0.0	632
MsG0880044960.01.T01	MTR_3g010820	79.853	407	31	5	5	362	47	451	0.0	627
MsG0880044960.01.T01	MTR_3g086440	63.480	408	95	7	5	362	47	450	5.87e-175	495
MsG0880044960.01.T01	MTR_8g042780	59.453	402	111	5	9	362	47	444	2.54e-170	484
MsG0880044960.01.T01	MTR_8g042770	58.374	406	119	6	5	362	48	451	1.47e-167	477
MsG0880044960.01.T01	MTR_8g042870	58.458	402	115	5	9	362	51	448	1.02e-166	474
MsG0880044960.01.T01	MTR_3g010850	56.934	411	123	7	2	362	28	434	1.96e-160	458
MsG0880044960.01.T01	MTR_8g042780	58.247	388	110	5	9	348	47	430	7.76e-160	455
MsG0880044960.01.T01	MTR_3g010850	57.143	406	121	7	5	362	46	446	4.31e-159	455
MsG0880044960.01.T01	MTR_8g042820	61.620	284	92	4	77	347	34	313	7.16e-122	354

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044960.01.T01	AT3G43790	50.485	412	144	7	5	362	45	450	1.58e-130	382
MsG0880044960.01.T01	AT3G43790	50.485	412	144	7	5	362	45	450	1.58e-130	382
MsG0880044960.01.T01	AT3G43790	50.242	414	146	7	5	364	45	452	2.43e-130	382
MsG0880044960.01.T01	AT3G43790	50.242	414	146	7	5	364	45	452	2.43e-130	382
MsG0880044960.01.T01	AT5G13750	48.921	417	145	7	3	362	44	449	4.03e-130	381
MsG0880044960.01.T01	AT5G13740	48.049	410	157	6	8	362	49	457	1.65e-129	380
MsG0880044960.01.T01	AT5G13740	47.837	393	149	6	8	345	49	440	6.82e-121	357
MsG0880044960.01.T01	AT5G13750	46.866	367	140	6	47	362	1	363	4.81e-108	322
MsG0880044960.01.T01	AT5G13750	46.486	370	148	5	3	324	44	411	1.49e-106	319
MsG0880044960.01.T01	AT5G13750	46.486	370	148	5	3	324	44	411	1.49e-106	319

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Find 88 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATTGGTCCAGCTGG+AGG	0.629594	8:+52091935	MsG0880044960.01.T01:CDS
AAACAGGAACAACGTCAAAG+AGG	0.605114	8:+52091872	MsG0880044960.01.T01:intron
AAAGCTGATATTGAAATGGA+AGG	0.590059	8:-52089814	None:intergenic
AACAGGAACAACGTCAAAGA+GGG	0.500301	8:+52091873	MsG0880044960.01.T01:intron
AACTTCACAACTGAGAATGT+TGG	0.592242	8:+52090954	MsG0880044960.01.T01:CDS
AAGATTTGCTCGCATCTCCG+GGG	0.729111	8:+52091170	MsG0880044960.01.T01:CDS
AAGTGCTTATGCTGGTTATG+TGG	0.512014	8:+52088219	MsG0880044960.01.T01:CDS
AATCCTTTGGAAATAGATTT+GGG	0.333774	8:-52089649	None:intergenic
AATTAGTTGCACATCATACC+TGG	0.443351	8:-52092231	None:intergenic
AATTGCCTGCATCTGGCTTC+CGG	0.470962	8:+52089852	MsG0880044960.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCTGATATTGAAATGGA+AGG	-	Chr8:52089817-52089836	None:intergenic	30.0%
AATTCAAATTACCTCGAGAA+CGG	-	Chr8:52091521-52091540	None:intergenic	30.0%
ACATCATTCTACTTCTTGAT+AGG	+	Chr8:52090887-52090906	MsG0880044960.01.T01:intron	30.0%
ACATCATTGTATTACTTGGA+TGG	+	Chr8:52091706-52091725	MsG0880044960.01.T01:CDS	30.0%
ATAAAAAGGGAAGTAAAAGC+AGG	-	Chr8:52090290-52090309	None:intergenic	30.0%
ATAATCCCTAAAATTGCAAC+AGG	-	Chr8:52088415-52088434	None:intergenic	30.0%
ATACCCAAATCTATTTCCAA+AGG	+	Chr8:52089646-52089665	MsG0880044960.01.T01:CDS	30.0%
ATCCAATCAGATATACATAC+CGG	-	Chr8:52089874-52089893	None:intergenic	30.0%
ATGTGGTATCAGTAAAAGTA+GGG	+	Chr8:52089950-52089969	MsG0880044960.01.T01:intron	30.0%
CAAAACATAAATCTTGTTGC+AGG	+	Chr8:52089776-52089795	MsG0880044960.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	52087947	52092251	52087947	ID=MsG0880044960.01;Name=MsG0880044960.01
Chr8	mRNA	52087947	52092251	52087947	ID=MsG0880044960.01.T01;Parent=MsG0880044960.01;Name=MsG0880044960.01.T01;_AED=0.41;_eAED=0.42;_QI=0\|0.83\|0.61\|1\|0.66\|0.61\|13\|0\|366
Chr8	exon	52087947	52088012	52087947	ID=MsG0880044960.01.T01:exon:22226;Parent=MsG0880044960.01.T01
Chr8	exon	52088181	52088241	52088181	ID=MsG0880044960.01.T01:exon:22227;Parent=MsG0880044960.01.T01
Chr8	exon	52088338	52088438	52088338	ID=MsG0880044960.01.T01:exon:22228;Parent=MsG0880044960.01.T01
Chr8	exon	52088973	52089026	52088973	ID=MsG0880044960.01.T01:exon:22229;Parent=MsG0880044960.01.T01
Chr8	exon	52089331	52089393	52089331	ID=MsG0880044960.01.T01:exon:22230;Parent=MsG0880044960.01.T01
Chr8	exon	52089635	52089684	52089635	ID=MsG0880044960.01.T01:exon:22231;Parent=MsG0880044960.01.T01
Chr8	exon	52089798	52089873	52089798	ID=MsG0880044960.01.T01:exon:22232;Parent=MsG0880044960.01.T01
Chr8	exon	52090388	52090579	52090388	ID=MsG0880044960.01.T01:exon:22233;Parent=MsG0880044960.01.T01
Chr8	exon	52090909	52090996	52090909	ID=MsG0880044960.01.T01:exon:22234;Parent=MsG0880044960.01.T01
Chr8	exon	52091106	52091211	52091106	ID=MsG0880044960.01.T01:exon:22235;Parent=MsG0880044960.01.T01
Chr8	exon	52091687	52091780	52091687	ID=MsG0880044960.01.T01:exon:22236;Parent=MsG0880044960.01.T01
Chr8	exon	52091878	52091966	52091878	ID=MsG0880044960.01.T01:exon:22237;Parent=MsG0880044960.01.T01
Chr8	exon	52092191	52092251	52092191	ID=MsG0880044960.01.T01:exon:22238;Parent=MsG0880044960.01.T01
Chr8	CDS	52087947	52088012	52087947	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52088181	52088241	52088181	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52088338	52088438	52088338	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52088973	52089026	52088973	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52089331	52089393	52089331	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52089635	52089684	52089635	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52089798	52089873	52089798	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52090388	52090579	52090388	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52090909	52090996	52090909	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52091106	52091211	52091106	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52091687	52091780	52091687	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52091878	52091966	52091878	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01
Chr8	CDS	52092191	52092251	52092191	ID=MsG0880044960.01.T01:cds;Parent=MsG0880044960.01.T01

Gene Sequence

>MsG0880044960.01.T01

ATGTCATTGTATTTGTGCTTTACAGCTCTGCCTATATCATCTCTCTTTCCTTACCAATATTTCATGATAAGAGATTTTAATATCGCAGAAAGGGAGGAGGACATAAGTGCTTATGCTGGTTATGTGGGATCTGCATTCATGCTTGGAAGATCTTTGACATCAATATTGTGGGGAGTCATAGCAGATCGTTACGGTAGAAAACCTGTTGCAATTTTAGGGATTATCTCGGCTTACTGCTCTGAAATTTTCCGAGAAGAAAAGCAAGCTCTTGGACTCTCAACTTTGAGTGCAGCTTGGGGCATAGGTTTAATAATTGGACCAGCTTTGGGAGGTTATTTGGCTCAGCCAACTGTAAAATACCCAAATCTATTTCCAAAGGATTCTTTTTGGGACAAGTTTCCATATTTCTTGCCTTCCATTTCAATATCAGCTTTTGCGTTTGCAGCAGCAATTGCCTGCATCTGGCTTCCGGAAACATTACACAACCACCCTCTTAGCAATGAGTCCATTGATGATGCTGAGGCTTTAGAAACTGGAAGAAATGGAAGAGCAGGCAAAGACAAGATAATCCAAAAAGATGAAAACCTCTTCCTAAACTGGCCCTTAATGTCATCTATTGTTGTGTATTGCATTTTCTCACTTTATAATATTTCTTATCAAGAGGTCTTCTCATTATGGGCGGTTAGTCCTAGAAGGTTAGGTGGTTTGAACTTCACAACTGAGAATGTTGGTGATGTTCTTGCAATATCAGGTATTGGTCTTATTGTCTACCAGCTTTCCCTATACCCATCATTAGAGAAAACATTTGGACCTGTAAGATTTGCTCGCATCTCCGGGGTAATATTTCACCATTCATATTATTTATATTACAAGCTTACATCATTGTATTACTTGGATGGATTTCAGGCGACCATAATCACTGGCTTATTTCTAATGCAAAATAGAGCAGTGGAACAACGTCAAAGAGGGGCAGCTAATGGCATTGCTATGACTGGAATGTCAATATTCAAAACAATTGGTCCAGCTGGAGGTGGTGCAGTATTAGCTTGGTCACAAAAACGGATGAATGCATCTTTCCTCCCAGGTATGATGTGCAACTAA

Protein sequence

>MsG0880044960.01.T01

MSLYLCFTALPISSLFPYQYFMIRDFNIAEREEDISAYAGYVGSAFMLGRSLTSILWGVIADRYGRKPVAILGIISAYCSEIFREEKQALGLSTLSAAWGIGLIIGPALGGYLAQPTVKYPNLFPKDSFWDKFPYFLPSISISAFAFAAAIACIWLPETLHNHPLSNESIDDAEALETGRNGRAGKDKIIQKDENLFLNWPLMSSIVVYCIFSLYNISYQEVFSLWAVSPRRLGGLNFTTENVGDVLAISGIGLIVYQLSLYPSLEKTFGPVRFARISGVIFHHSYYLYYKLTSLYYLDGFQATIITGLFLMQNRAVEQRQRGAANGIAMTGMSIFKTIGPAGGGAVLAWSQKRMNASFLPGMMCN*