AlfalfaGEDB | MsG0880045062.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045062.01

KEGG: K16287
GO: GO:0006508 GO:0008234
PFAM: PF02902

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045062.01.T01	XP_024628466.1	91.503	153	13	0	1	153	1	153	1.51E-85	285
MsG0880045062.01.T01	XP_024628466.1	95.364	151	7	0	280	430	251	401	2.25E-81	274
MsG0880045062.01.T02	XP_013445975.1	85.211	284	21	2	19	283	383	664	6.99E-163	478

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045062.01.T01	Q2PS26	49.635	137	65	2	297	430	301	436	3.24E-41	157
MsG0880045062.01.T02	Q2PS26	58.503	147	58	1	38	184	437	580	3.26E-56	193

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045062.01.T01	A0A396GRP8	91.503	153	13	0	1	153	1	153	7.22e-86	285

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023202.01	MsG0880045062.01	0.804161	2.467355e-49	1.263143e-46
MsG0580024038.01	MsG0880045062.01	0.815932	7.176242e-52	5.014897e-49

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045062.01.T01	MTR_8g064470	90.196	153	13	1	1	153	1	151	2.81e-87	274
MsG0880045062.01.T01	MTR_8g064470	95.364	151	7	0	280	430	249	399	2.56e-86	271
MsG0880045062.01.T01	MTR_8g064470	90.196	153	13	1	1	153	1	151	5.13e-87	274
MsG0880045062.01.T01	MTR_8g064470	95.364	151	7	0	280	430	249	399	5.65e-86	271
MsG0880045062.01.T01	MTR_8g064470	90.196	153	13	1	1	153	1	151	3.60e-86	276
MsG0880045062.01.T01	MTR_8g064470	95.364	151	7	0	280	430	249	399	2.63e-85	274
MsG0880045062.01.T01	MTR_8g064370	66.667	87	27	2	37	122	5	90	7.67e-25	106
MsG0880045062.01.T01	MTR_8g064370	40.351	171	74	6	24	175	223	384	1.35e-21	97.1
MsG0880045062.01.T01	MTR_8g064370	65.672	67	23	0	37	103	166	232	7.39e-18	85.9
MsG0880045062.01.T01	MTR_8g064370	70.213	47	14	0	57	103	121	167	8.37e-11	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045062.01.T01	AT1G60220	49.635	137	65	2	297	430	301	436	3.30e-42	157
MsG0880045062.01.T01	AT1G10570	46.099	141	68	4	296	430	155	293	5.16e-33	129
MsG0880045062.01.T01	AT1G10570	46.099	141	68	4	296	430	285	423	1.48e-32	130
MsG0880045062.01.T01	AT1G10570	46.099	141	68	4	296	430	286	424	1.55e-32	130
MsG0880045062.01.T02	AT1G60220	58.503	147	58	1	38	184	437	580	3.32e-57	193
MsG0880045062.01.T02	AT1G10570	51.163	172	74	5	19	188	277	440	3.08e-52	177
MsG0880045062.01.T02	AT1G10570	51.163	172	74	5	19	188	408	571	2.86e-51	177
MsG0880045062.01.T02	AT1G10570	51.163	172	74	5	19	188	407	570	3.00e-51	177
MsG0880045062.01.T02	AT1G09730	35.802	162	78	8	36	183	518	667	1.01e-17	83.6
MsG0880045062.01.T02	AT1G09730	35.802	162	78	8	36	183	486	635	1.19e-17	83.6

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Find 225 sgRNAs with CRISPR-Local

Find 917 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTACTCCTGGTGTAT+TGG	0.386530	8:-53646235	None:intergenic
AAAAGTCGAAACATAATATA+AGG	0.342040	8:-53646444	None:intergenic
AAACATGAAATATTGTAAAC+AGG	0.427970	8:-53647008	None:intergenic
AAACTGCAAAGGAACTTTGA+TGG	0.387115	8:+53647191	MsG0880045062.01.T01:three_prime_UTR
AAAGAGTGCTGGGCGTGAGG+GGG	0.458174	8:+53648139	MsG0880045062.01.T01:three_prime_UTR
AAAGCCCTATCTCACTAAGT+GGG	0.574546	8:-53647925	None:intergenic
AAAGGGGAGCTGTGGCACTA+GGG	0.532517	8:-53647462	None:intergenic
AAAGTTCAGAAGGTGGTGGA+AGG	0.533933	8:+53646177	MsG0880045062.01.T01:CDS
AAATTCCGCTAAGAATTTGC+CGG	0.429482	8:+53639138	MsG0880045062.01.T01:CDS
AACGGAGCCGGCTCCTGATC+GGG	0.411109	8:-53639043	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAGGGGCAATATTTC+GGG	-	Chr8:53651606-53651625	None:intergenic	30.0%
AACACCAAAATAGTACGAAA+TGG	-	Chr8:53649484-53649503	None:intergenic	30.0%
AAGTCAGTTTGAATTCAGTT+CGG	+	Chr8:53646697-53646716	MsG0880045062.01.T01:three_prime_UTR	30.0%
AAGTTAAGAGACTAATTTGG+GGG	+	Chr8:53639972-53639991	MsG0880045062.01.T01:intron	30.0%
AATACAAAATCCTCCCAATA+TGG	-	Chr8:53641585-53641604	None:intergenic	30.0%
AATATAAAGGGATGAGGAAA+GGG	-	Chr8:53640809-53640828	None:intergenic	30.0%
AATGGTTCACTGTTTAAGTA+TGG	+	Chr8:53647386-53647405	MsG0880045062.01.T01:three_prime_UTR	30.0%
AATGTAGAATTAGCATAGGA+AGG	-	Chr8:53639258-53639277	None:intergenic	30.0%
AATTGTAGGGATGATAGATA+TGG	-	Chr8:53643246-53643265	None:intergenic	30.0%
ACACCAAAATAGTACGAAAT+GGG	-	Chr8:53649483-53649502	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	53638941	53651888	53638941	ID=MsG0880045062.01;Name=MsG0880045062.01
Chr8	mRNA	53638941	53651888	53638941	ID=MsG0880045062.01.T01;Parent=MsG0880045062.01;Name=MsG0880045062.01.T01;_AED=0.33;_eAED=0.34;_QI=0\|0.66\|0.61\|1\|0.58\|0.53\|13\|3691\|430
Chr8	exon	53638941	53639246	53638941	ID=MsG0880045062.01.T01:exon:23080;Parent=MsG0880045062.01.T01
Chr8	exon	53640103	53640157	53640103	ID=MsG0880045062.01.T01:exon:23081;Parent=MsG0880045062.01.T01
Chr8	exon	53642221	53642320	53642221	ID=MsG0880045062.01.T01:exon:23082;Parent=MsG0880045062.01.T01
Chr8	exon	53642405	53642682	53642405	ID=MsG0880045062.01.T01:exon:23083;Parent=MsG0880045062.01.T01
Chr8	exon	53643847	53643922	53643847	ID=MsG0880045062.01.T01:exon:23084;Parent=MsG0880045062.01.T01
Chr8	exon	53644023	53644175	53644023	ID=MsG0880045062.01.T01:exon:23085;Parent=MsG0880045062.01.T01
Chr8	exon	53644272	53644304	53644272	ID=MsG0880045062.01.T01:exon:23086;Parent=MsG0880045062.01.T01
Chr8	exon	53644393	53644488	53644393	ID=MsG0880045062.01.T01:exon:23087;Parent=MsG0880045062.01.T01
Chr8	exon	53644764	53644857	53644764	ID=MsG0880045062.01.T01:exon:23088;Parent=MsG0880045062.01.T01
Chr8	exon	53646151	53648653	53646151	ID=MsG0880045062.01.T01:exon:23089;Parent=MsG0880045062.01.T01
Chr8	CDS	53638941	53639246	53638941	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53640103	53640157	53640103	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53642221	53642320	53642221	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53642405	53642682	53642405	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53643847	53643922	53643847	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53644023	53644175	53644023	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53644272	53644304	53644272	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53644393	53644488	53644393	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53644764	53644857	53644764	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	CDS	53646151	53646252	53646151	ID=MsG0880045062.01.T01:cds;Parent=MsG0880045062.01.T01
Chr8	three_prime_UTR	53646253	53648653	53646253	ID=MsG0880045062.01.T01:three_prime_utr;Parent=MsG0880045062.01.T01
Chr8	three_prime_UTR	53649673	53649787	53649673	ID=MsG0880045062.01.T01:three_prime_utr;Parent=MsG0880045062.01.T01
Chr8	three_prime_UTR	53650637	53650753	53650637	ID=MsG0880045062.01.T01:three_prime_utr;Parent=MsG0880045062.01.T01
Chr8	three_prime_UTR	53650831	53651888	53650831	ID=MsG0880045062.01.T01:three_prime_utr;Parent=MsG0880045062.01.T01
Chr8	mRNA	53648198	53651888	53648198	ID=MsG0880045062.01.T02;Parent=MsG0880045062.01;Name=MsG0880045062.01.T02;_AED=0.45;_eAED=0.45;_QI=0\|0.75\|0.6\|1\|0.75\|0.8\|5\|671\|283
Chr8	exon	53648198	53648257	53648198	ID=MsG0880045062.01.T02:exon:23093;Parent=MsG0880045062.01.T02
Chr8	exon	53648481	53648653	53648481	ID=MsG0880045062.01.T02:exon:23094;Parent=MsG0880045062.01.T02
Chr8	CDS	53648198	53648257	53648198	ID=MsG0880045062.01.T02:cds;Parent=MsG0880045062.01.T02
Chr8	CDS	53648481	53648653	53648481	ID=MsG0880045062.01.T02:cds;Parent=MsG0880045062.01.T02
Chr8	CDS	53649673	53649787	53649673	ID=MsG0880045062.01.T02:cds;Parent=MsG0880045062.01.T02
Chr8	CDS	53650637	53650753	53650637	ID=MsG0880045062.01.T02:cds;Parent=MsG0880045062.01.T02
Chr8	CDS	53650831	53651217	53650831	ID=MsG0880045062.01.T02:cds;Parent=MsG0880045062.01.T02
Chr8	three_prime_UTR	53651218	53651888	53651218	ID=MsG0880045062.01.T02:three_prime_utr;Parent=MsG0880045062.01.T02

Gene Sequence

>MsG0880045062.01.T02

ATGAGACTCTTGAGAAGAGTTTATAAGGAGGAGGCAATCCTCACCTTACAAGCCGGTTTTAACCTCTACTCATATATGGATTTATTGCTTGTGATGTTTCATGTGTTCAGTCTTCATTGGAGTTTGATCATTATTTGTATCCCGGACAAAGATGATGAATCAGGACCGATCATACTTCATTTGGATTCTTTGGGTCTTCACTCGAGTCGATCAGTTTTTGATAATATTAAAAGATATTTGATTGAAGAAAAGAACTATTTGAGTCGAGAATGCGCGTCTACAGATGTTCCGATGGCAGACAGAATATGGAAGAGTCTTTCTCGTAGAATTGAAACTCAAGTCATAGCCGTTCCCCAACAGAAGAATGAGTATGACTGTGGTCTTTTTGTACTGTATTTCATTGAGCGTTTCATAGGAGAGGCACCTGAAAGACTGAAAAAGAAAGATTTAACAAATAATCAGAGGTTTGGCAAGCGATGGTTCAAACCTGAAGAGGCATCCAGTTTGAGAGTGAAAATCCACAAGTTGCTTGTAGCAGAACTACGTAATTCAATCAAGCGTGATGGCATCACAGAATCTTCTCCCTCATCTTCTGGCGCTGCAACTGAATGCGTTGAGACTGCCAAGACAGAATCGTCTCTGTCATCTGCTGGCGCTGCAACTGAATGCGTTGAGACTGCCAAGACAGAATCTTCTCCGTCATCTGCTGGCGCTGCAATCGAATGTGTTGAGATTGCCAAGACAGAATCTTCGCCGTCATCTGCTGGTGTTGCAAATGAATGTGTTGAGACTGCCAAGGACTCTTCGATCGAAGATGGCATCACGAGTTGCAATTCTGACATTATTGAATGA

>MsG0880045062.01.T01

ATGGAGCAAAAACAGAAAGGTCCGCTTCCTCTCAACTTGGACGCGCTTGTCGACAATACGCCGGCGGCGGAACTCGTCGTCAAACCGTCTGTTACGGTGGTTCCCGATCAGGAGCCGGCTCCGTTCGTTGGCGGCCTGGACCGCCTCAAAGATTACGAGCTCGTTGATCACATTCTGAGAAAGAAGAAACTTCTTGAAAATTCCGCTAAGAATTTGCCGGATAAAGGTGCAAAACTTCGCGCTCTCATCAAGAGCTATGAAGATGAGGTTCATCGCCGGAAAGTTAACCCTCGTCCTCAGGTTCAGAAAGTTATTGATGTGGAGCAGAAGCCAAGACAGGCTACAAGCTCAAGTGCTGTTGGTGTATCTAATTACTCGAGACAGGAGAATTTGCCATTTCGTCCACAGTCAGAAGCCTCTTTAGCATCTAATATTATGAATAAAAGAGACAATAATGTAAGACTAATTGTACAGATGTTGATGCATTTAGCAATGAGATGCCACATTTCAAGCATTGCAACAACCAGACAGTACGACTCAACAGAGAACCTAAAAGAAGAAAGAGACATCGATCATCTACAAGAAAATTGCCTTATCAATGCCCTAACAAACTTTCCAAACGTGATACTTTTAATGATGATAAAAGGTTCCGATCCAATTCCACCTTATCCTTACAGAACATTGCAAGGAATCTACAAAGACAGATTTCGAAAGAGTAAGAGAAAATGCTTTTCTTATGTAAGGGTGCATTTCAGACAAGTCAATCAGATGGCTCAAGGTCCAGGAAGGTGTGTTTGCATAGCTAATAAACTTTGTGATATGTTATATATGACGTGTTCTAAACAGGGTCATGTTCAACCCATTGTTCTTGATGTGGACGACGACGATGAAGACGACATTGAAGACGACGAAGATGACCGCGGAGATCCTCATATTCTGGATAAAACAGAAAATAAAGTTCCTGAATATTTGAAAGAAGCTAAGGTTTATTTTCCATCACGAGATGATCCCGAGTGTGTTGAAATTTGTTACAATGACATGGAATGCCTTGCTCCTGAAGGCTACTTAACATCAACTATCATGAACTTTTACATACGATATTTGCAGCAGCAAGTATCTCTGACAACATCTGACTGCCATTTTTTCAACACATATTTCTACAAGAAACTTAAAGAAGCTGTCTCCTGCAAGGAAAGCAACAAAGAGACAATTTTTTCAAAGTTCAGAAGGTGGTGGAAGGGTGTAAATCTTTTTGAGAAGGCATATGTTTTGATTCCAATACACCAGGAGTAA

Protein sequence

>MsG0880045062.01.T02

MRLLRRVYKEEAILTLQAGFNLYSYMDLLLVMFHVFSLHWSLIIICIPDKDDESGPIILHLDSLGLHSSRSVFDNIKRYLIEEKNYLSRECASTDVPMADRIWKSLSRRIETQVIAVPQQKNEYDCGLFVLYFIERFIGEAPERLKKKDLTNNQRFGKRWFKPEEASSLRVKIHKLLVAELRNSIKRDGITESSPSSSGAATECVETAKTESSLSSAGAATECVETAKTESSPSSAGAAIECVEIAKTESSPSSAGVANECVETAKDSSIEDGITSCNSDIIE*

>MsG0880045062.01.T01

MEQKQKGPLPLNLDALVDNTPAAELVVKPSVTVVPDQEPAPFVGGLDRLKDYELVDHILRKKKLLENSAKNLPDKGAKLRALIKSYEDEVHRRKVNPRPQVQKVIDVEQKPRQATSSSAVGVSNYSRQENLPFRPQSEASLASNIMNKRDNNVRLIVQMLMHLAMRCHISSIATTRQYDSTENLKEERDIDHLQENCLINALTNFPNVILLMMIKGSDPIPPYPYRTLQGIYKDRFRKSKRKCFSYVRVHFRQVNQMAQGPGRCVCIANKLCDMLYMTCSKQGHVQPIVLDVDDDDEDDIEDDEDDRGDPHILDKTENKVPEYLKEAKVYFPSRDDPECVEICYNDMECLAPEGYLTSTIMNFYIRYLQQQVSLTTSDCHFFNTYFYKKLKEAVSCKESNKETIFSKFRRWWKGVNLFEKAYVLIPIHQE*