Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045451.01.T01 | RHN78823.1 | 54.331 | 254 | 86 | 5 | 1 | 252 | 1 | 226 | 9.35E-62 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045451.01.T01 | A0A396JRA7 | 54.331 | 254 | 86 | 5 | 1 | 252 | 1 | 226 | 4.47e-62 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000085.01 | MsG0880045451.01 | 0.819051 | 1.422479e-52 | 1.082992e-49 |
| MsG0880045451.01 | MsG0880047403.01 | 0.825064 | 5.746425e-54 | 5.182409e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045451.01.T01 | MTR_1g025830 | 54.545 | 253 | 85 | 4 | 1 | 252 | 1 | 224 | 4.37e-65 | 202 |
| MsG0880045451.01.T01 | MTR_1g025810 | 52.778 | 252 | 110 | 3 | 1 | 251 | 1 | 244 | 1.17e-59 | 189 |
| MsG0880045451.01.T01 | MTR_1g048260 | 53.448 | 232 | 79 | 4 | 1 | 231 | 1 | 204 | 1.34e-56 | 181 |
| MsG0880045451.01.T01 | MTR_5g048270 | 44.130 | 247 | 115 | 3 | 7 | 252 | 3 | 227 | 1.72e-49 | 163 |
| MsG0880045451.01.T01 | MTR_2g020500 | 44.538 | 119 | 54 | 2 | 1 | 118 | 12 | 119 | 1.08e-23 | 95.9 |
| MsG0880045451.01.T01 | MTR_2g021480 | 63.158 | 76 | 27 | 1 | 1 | 75 | 12 | 87 | 1.40e-22 | 94.0 |
| MsG0880045451.01.T01 | MTR_2g021560 | 52.174 | 92 | 42 | 2 | 1 | 91 | 1 | 91 | 6.19e-22 | 92.0 |
| MsG0880045451.01.T01 | MTR_2g021590 | 56.579 | 76 | 32 | 1 | 1 | 75 | 5 | 80 | 7.84e-21 | 88.2 |
| MsG0880045451.01.T01 | MTR_3g087310 | 56.522 | 69 | 29 | 1 | 9 | 76 | 9 | 77 | 1.93e-20 | 82.8 |
| MsG0880045451.01.T01 | MTR_2g021510 | 56.522 | 69 | 29 | 1 | 8 | 75 | 18 | 86 | 1.77e-19 | 83.2 |
| MsG0880045451.01.T01 | MTR_2g021570 | 55.738 | 61 | 26 | 1 | 10 | 69 | 32 | 92 | 2.49e-17 | 75.5 |
| MsG0880045451.01.T01 | MTR_2g021530 | 53.623 | 69 | 31 | 1 | 8 | 75 | 4 | 72 | 4.38e-17 | 75.5 |
| MsG0880045451.01.T01 | MTR_2g021660 | 54.795 | 73 | 32 | 1 | 1 | 72 | 2 | 74 | 7.36e-17 | 76.3 |
| MsG0880045451.01.T01 | MTR_2g022750 | 48.571 | 70 | 35 | 1 | 8 | 76 | 11 | 80 | 8.38e-17 | 76.6 |
| MsG0880045451.01.T01 | MTR_2g022750 | 48.571 | 70 | 35 | 1 | 8 | 76 | 11 | 80 | 1.08e-16 | 76.3 |
| MsG0880045451.01.T01 | MTR_2g021450 | 49.315 | 73 | 36 | 1 | 1 | 72 | 1 | 73 | 1.27e-16 | 74.7 |
| MsG0880045451.01.T01 | MTR_2g021600 | 48.438 | 128 | 57 | 3 | 2 | 127 | 3 | 123 | 1.17e-15 | 74.7 |
| MsG0880045451.01.T01 | MTR_2g021630 | 44.660 | 103 | 44 | 2 | 1 | 102 | 1 | 91 | 2.64e-15 | 72.0 |
| MsG0880045451.01.T01 | MTR_0485s0020 | 51.316 | 76 | 36 | 1 | 1 | 75 | 1 | 76 | 8.59e-15 | 70.9 |
| MsG0880045451.01.T01 | MTR_2g021640 | 52.143 | 140 | 54 | 3 | 9 | 146 | 39 | 167 | 1.81e-11 | 62.0 |
| MsG0880045451.01.T01 | MTR_6g460450 | 58.140 | 43 | 18 | 0 | 12 | 54 | 28 | 70 | 7.94e-11 | 58.9 |
| MsG0880045451.01.T01 | MTR_1g025850 | 29.339 | 242 | 104 | 6 | 13 | 252 | 27 | 203 | 8.18e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGATAGAATGGTATTAGA+AGG | 0.329988 | 8:-59305930 | MsG0880045451.01.T01:CDS |
| CGAGGAAGAGGAAGAGGAAG+AGG | 0.385217 | 8:-59306252 | MsG0880045451.01.T01:CDS |
| GAAGAAGAAGGGAGAAAATC+TGG | 0.388008 | 8:-59306069 | MsG0880045451.01.T01:CDS |
| AGAGGAAGTGGAAGAGGAAG+AGG | 0.393476 | 8:-59306315 | MsG0880045451.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.394447 | 8:-59306246 | MsG0880045451.01.T01:CDS |
| AGAAGAAGAGGAAGTGGAAG+AGG | 0.394857 | 8:-59306321 | MsG0880045451.01.T01:CDS |
| AGAGGAAGAAGAAGAGGAAG+AGG | 0.396271 | 8:-59306285 | MsG0880045451.01.T01:CDS |
| AGTGGAAGAGGAAGAGGAAG+AGG | 0.420722 | 8:-59306309 | MsG0880045451.01.T01:CDS |
| GATAATCAAGGTGGCTAAGT+TGG | 0.425265 | 8:-59306024 | MsG0880045451.01.T01:CDS |
| TTCAAATTGCGTATCAAAGA+AGG | 0.426091 | 8:-59306359 | MsG0880045451.01.T01:CDS |
| AGAGGAAGAGGAAGAGGAAG+AGG | 0.426997 | 8:-59306303 | MsG0880045451.01.T01:CDS |
| CAAGAATGAAATGCAAACTA+TGG | 0.428127 | 8:-59305892 | MsG0880045451.01.T01:CDS |
| ATTTCGATCAACAGTTCATC+CGG | 0.433150 | 8:+59306539 | None:intergenic |
| GGAAGACGAAGACGAGGAAG+AGG | 0.455608 | 8:-59306264 | MsG0880045451.01.T01:CDS |
| CGAAGACGAGGAAGAGGAAG+AGG | 0.461269 | 8:-59306258 | MsG0880045451.01.T01:CDS |
| AGAGAACCACTTGAAGAAGA+AGG | 0.464660 | 8:-59306081 | MsG0880045451.01.T01:CDS |
| AGAGGAAGAGGAAGAAGAAG+AGG | 0.467774 | 8:-59306291 | MsG0880045451.01.T01:CDS |
| TTTCTCCCTTCTTCTTCAAG+TGG | 0.481048 | 8:+59306075 | None:intergenic |
| TAAGTTGGATAATTATTTGA+TGG | 0.483560 | 8:-59306009 | MsG0880045451.01.T01:CDS |
| ACCACCCTTGCTTCCGCCAT+CGG | 0.486775 | 8:+59306581 | None:intergenic |
| TGCAAACGCACCTCAACTCC+AGG | 0.501489 | 8:+59306489 | None:intergenic |
| TTCTTGAATCAACTCTTGAA+AGG | 0.507047 | 8:+59306516 | None:intergenic |
| TGAGGTGCGTTTGCAAGTTG+TGG | 0.509786 | 8:-59306481 | MsG0880045451.01.T01:CDS |
| GATAGTGTTGATGTTGATGC+TGG | 0.519920 | 8:-59306167 | MsG0880045451.01.T01:CDS |
| CAATTCTCTAGAGGATAGAA+TGG | 0.530815 | 8:-59305940 | MsG0880045451.01.T01:CDS |
| TGTTGATGCTGGAGAGAAAG+AGG | 0.532391 | 8:-59306156 | MsG0880045451.01.T01:CDS |
| GTCACCAAACAGTGTACTCC+CGG | 0.545120 | 8:-59306558 | MsG0880045451.01.T01:CDS |
| TCATCCGGGAGTACACTGTT+TGG | 0.546949 | 8:+59306554 | None:intergenic |
| TCCGATGGCGGAAGCAAGGG+TGG | 0.547946 | 8:-59306582 | None:intergenic |
| GAGAACCACTTGAAGAAGAA+GGG | 0.552372 | 8:-59306080 | MsG0880045451.01.T01:CDS |
| CAAGAAATCACCTGGAGTTG+AGG | 0.559238 | 8:-59306499 | MsG0880045451.01.T01:CDS |
| CAAGTCTATCAATTCTCTAG+AGG | 0.569018 | 8:-59305949 | MsG0880045451.01.T01:CDS |
| AGGTGATTCTTCATGAAATG+AGG | 0.569527 | 8:+59306440 | None:intergenic |
| TGCACAATGGATGATAATCA+AGG | 0.575904 | 8:-59306036 | MsG0880045451.01.T01:CDS |
| TCTCGTTACTCTAAGTCGTA+AGG | 0.576711 | 8:-59306402 | MsG0880045451.01.T01:CDS |
| GAAAGCATTGAAGTGTTCAA+CGG | 0.580568 | 8:+59306379 | None:intergenic |
| AGTTGATTCAAGAAATCACC+TGG | 0.581636 | 8:-59306507 | MsG0880045451.01.T01:CDS |
| AGAAGAAGAGCAGATGAACT+CGG | 0.582235 | 8:-59306132 | MsG0880045451.01.T01:CDS |
| GGAAGAGGAAGACGAAGACG+AGG | 0.584300 | 8:-59306270 | MsG0880045451.01.T01:CDS |
| AGGATAGAATGGTATTAGAA+GGG | 0.598536 | 8:-59305929 | MsG0880045451.01.T01:CDS |
| TTTCGATCAACAGTTCATCC+GGG | 0.605533 | 8:+59306540 | None:intergenic |
| TGGATAATCTCAATGCACAA+TGG | 0.628707 | 8:-59306049 | MsG0880045451.01.T01:CDS |
| GAATGAAATGCAAACTATGG+AGG | 0.629276 | 8:-59305889 | MsG0880045451.01.T01:CDS |
| AAATTGCGTATCAAAGAAGG+AGG | 0.641497 | 8:-59306356 | MsG0880045451.01.T01:CDS |
| ACGACTTAGAGTAACGAGAT+CGG | 0.648242 | 8:+59306406 | None:intergenic |
| CGAGATCGGTTAACGAAACG+AGG | 0.655307 | 8:+59306420 | None:intergenic |
| AGAAGAGCAGATGAACTCGG+TGG | 0.715453 | 8:-59306129 | MsG0880045451.01.T01:CDS |
| ACAATGGATGATAATCAAGG+TGG | 0.763777 | 8:-59306033 | MsG0880045451.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAGTTGGATAATTATTTGA+TGG | - | Chr8:59306411-59306430 | MsG0880045451.01.T01:CDS | 20.0% |
| AGGATAGAATGGTATTAGAA+GGG | - | Chr8:59306491-59306510 | MsG0880045451.01.T01:CDS | 30.0% | |
| CAAGAATGAAATGCAAACTA+TGG | - | Chr8:59306528-59306547 | MsG0880045451.01.T01:CDS | 30.0% | |
| TTCAAATTGCGTATCAAAGA+AGG | - | Chr8:59306061-59306080 | MsG0880045451.01.T01:CDS | 30.0% | |
| TTCTTGAATCAACTCTTGAA+AGG | + | Chr8:59305907-59305926 | None:intergenic | 30.0% | |
| AAATTGCGTATCAAAGAAGG+AGG | - | Chr8:59306064-59306083 | MsG0880045451.01.T01:CDS | 35.0% | |
| ACAATGGATGATAATCAAGG+TGG | - | Chr8:59306387-59306406 | MsG0880045451.01.T01:CDS | 35.0% | |
| AGAAGAAGAAGAAGAAGAAG+AGG | - | Chr8:59306087-59306106 | MsG0880045451.01.T01:CDS | 35.0% | |
| AGGTGATTCTTCATGAAATG+AGG | + | Chr8:59305983-59306002 | None:intergenic | 35.0% | |
| ATTTCGATCAACAGTTCATC+CGG | + | Chr8:59305884-59305903 | None:intergenic | 35.0% | |
| CAAGTCTATCAATTCTCTAG+AGG | - | Chr8:59306471-59306490 | MsG0880045451.01.T01:CDS | 35.0% | |
| GAAGAAGAAGAAGAAGAAGA+TGG | - | Chr8:59306088-59306107 | MsG0880045451.01.T01:CDS | 35.0% | |
| GAATGAAATGCAAACTATGG+AGG | - | Chr8:59306531-59306550 | MsG0880045451.01.T01:CDS | 35.0% | |
| GAGGATAGAATGGTATTAGA+AGG | - | Chr8:59306490-59306509 | MsG0880045451.01.T01:CDS | 35.0% | |
| TGCACAATGGATGATAATCA+AGG | - | Chr8:59306384-59306403 | MsG0880045451.01.T01:CDS | 35.0% | |
| TGGATAATCTCAATGCACAA+TGG | - | Chr8:59306371-59306390 | MsG0880045451.01.T01:CDS | 35.0% | |
| ! | AGTTGATTCAAGAAATCACC+TGG | - | Chr8:59305913-59305932 | MsG0880045451.01.T01:CDS | 35.0% |
| ! | CAATTCTCTAGAGGATAGAA+TGG | - | Chr8:59306480-59306499 | MsG0880045451.01.T01:CDS | 35.0% |
| !! | GAAAGCATTGAAGTGTTCAA+CGG | + | Chr8:59306044-59306063 | None:intergenic | 35.0% |
| ACGACTTAGAGTAACGAGAT+CGG | + | Chr8:59306017-59306036 | None:intergenic | 40.0% | |
| AGAAGAAGAAGAAGAGGAAG+TGG | - | Chr8:59306093-59306112 | MsG0880045451.01.T01:CDS | 40.0% | |
| AGAAGAAGAGCAGATGAACT+CGG | - | Chr8:59306288-59306307 | MsG0880045451.01.T01:CDS | 40.0% | |
| AGAGAACCACTTGAAGAAGA+AGG | - | Chr8:59306339-59306358 | MsG0880045451.01.T01:CDS | 40.0% | |
| GAAGAAGAAGGGAGAAAATC+TGG | - | Chr8:59306351-59306370 | MsG0880045451.01.T01:CDS | 40.0% | |
| GAGAACCACTTGAAGAAGAA+GGG | - | Chr8:59306340-59306359 | MsG0880045451.01.T01:CDS | 40.0% | |
| GATAATCAAGGTGGCTAAGT+TGG | - | Chr8:59306396-59306415 | MsG0880045451.01.T01:CDS | 40.0% | |
| TCTCGTTACTCTAAGTCGTA+AGG | - | Chr8:59306018-59306037 | MsG0880045451.01.T01:CDS | 40.0% | |
| TTTCGATCAACAGTTCATCC+GGG | + | Chr8:59305883-59305902 | None:intergenic | 40.0% | |
| TTTCTCCCTTCTTCTTCAAG+TGG | + | Chr8:59306348-59306367 | None:intergenic | 40.0% | |
| !! | GATAGTGTTGATGTTGATGC+TGG | - | Chr8:59306253-59306272 | MsG0880045451.01.T01:CDS | 40.0% |
| AGAAGAAGAGGAAGTGGAAG+AGG | - | Chr8:59306099-59306118 | MsG0880045451.01.T01:CDS | 45.0% | |
| AGAGGAAGAAGAAGAGGAAG+AGG | - | Chr8:59306135-59306154 | MsG0880045451.01.T01:CDS | 45.0% | |
| AGAGGAAGAGGAAGAAGAAG+AGG | - | Chr8:59306129-59306148 | MsG0880045451.01.T01:CDS | 45.0% | |
| CAAGAAATCACCTGGAGTTG+AGG | - | Chr8:59305921-59305940 | MsG0880045451.01.T01:CDS | 45.0% | |
| TGTTGATGCTGGAGAGAAAG+AGG | - | Chr8:59306264-59306283 | MsG0880045451.01.T01:CDS | 45.0% | |
| AGAAGAGCAGATGAACTCGG+TGG | - | Chr8:59306291-59306310 | MsG0880045451.01.T01:CDS | 50.0% | |
| AGAGGAAGAGGAAGAGGAAG+AGG | - | Chr8:59306117-59306136 | MsG0880045451.01.T01:CDS | 50.0% | |
| AGAGGAAGTGGAAGAGGAAG+AGG | - | Chr8:59306105-59306124 | MsG0880045451.01.T01:CDS | 50.0% | |
| AGTGGAAGAGGAAGAGGAAG+AGG | - | Chr8:59306111-59306130 | MsG0880045451.01.T01:CDS | 50.0% | |
| CGAGATCGGTTAACGAAACG+AGG | + | Chr8:59306003-59306022 | None:intergenic | 50.0% | |
| GTCACCAAACAGTGTACTCC+CGG | - | Chr8:59305862-59305881 | MsG0880045451.01.T01:CDS | 50.0% | |
| TGAGGTGCGTTTGCAAGTTG+TGG | - | Chr8:59305939-59305958 | MsG0880045451.01.T01:CDS | 50.0% | |
| ! | TCATCCGGGAGTACACTGTT+TGG | + | Chr8:59305869-59305888 | None:intergenic | 50.0% |
| CGAAGACGAGGAAGAGGAAG+AGG | - | Chr8:59306162-59306181 | MsG0880045451.01.T01:CDS | 55.0% | |
| CGAGGAAGAGGAAGAGGAAG+AGG | - | Chr8:59306168-59306187 | MsG0880045451.01.T01:CDS | 55.0% | |
| GGAAGACGAAGACGAGGAAG+AGG | - | Chr8:59306156-59306175 | MsG0880045451.01.T01:CDS | 55.0% | |
| GGAAGAGGAAGACGAAGACG+AGG | - | Chr8:59306150-59306169 | MsG0880045451.01.T01:CDS | 55.0% | |
| TGCAAACGCACCTCAACTCC+AGG | + | Chr8:59305934-59305953 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 59305842 | 59306600 | 59305842 | ID=MsG0880045451.01;Name=MsG0880045451.01 |
| Chr8 | mRNA | 59305842 | 59306600 | 59305842 | ID=MsG0880045451.01.T01;Parent=MsG0880045451.01;Name=MsG0880045451.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|252 |
| Chr8 | exon | 59305842 | 59306600 | 59305842 | ID=MsG0880045451.01.T01:exon:22824;Parent=MsG0880045451.01.T01 |
| Chr8 | CDS | 59305842 | 59306600 | 59305842 | ID=MsG0880045451.01.T01:cds;Parent=MsG0880045451.01.T01 |
| Gene Sequence |
| Protein sequence |
