AlfalfaGEDB | MsG0880045456.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045456.01

KEGG: -
GO: -
PFAM: PF13639

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045456.01.T01	XP_013446298.1	89.888	178	18	1	178	1	178	4.23E-112	327
MsG0880045456.01.T02	XP_013446298.1	89.809	157	16	1	157	22	178	2.11E-96	286
MsG0880045456.01.T03	XP_013446298.1	91.781	73	6	1	73	106	178	2.99E-41	142

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045456.01.T01	Q9LF64	36.585	164	82	5	17	162	59	218	7.95E-19	85.5
MsG0880045456.01.T02	Q9LF64	36.709	158	79	4	1	141	65	218	8.02E-19	84.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045456.01.T01	A0A072U3D3	89.888	178	18	1	178	1	178	2.02e-112	327
MsG0880045456.01.T02	A0A072U3D3	89.809	157	16	1	157	22	178	1.01e-96	286
MsG0880045456.01.T03	A0A072U3D3	91.781	73	6	1	73	106	178	1.43e-41	142

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0280010800.01	MsG0880045456.01	PPI
MsG0880045456.01	MsG0280010802.01	PPI
MsG0880045456.01	MsG0280010800.01	PPI
MsG0880045456.01	MsG0280010800.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045456.01.T01	MTR_8g071815	89.888	178	18	0	1	178	1	178	5.12e-116	327
MsG0880045456.01.T01	MTR_5g026730	54.483	145	58	6	4	141	2	145	8.92e-44	143
MsG0880045456.01.T01	MTR_5g026720	52.381	147	61	6	1	141	1	144	1.67e-42	139
MsG0880045456.01.T01	MTR_2g041200	38.636	132	69	5	19	144	33	158	1.63e-21	86.7
MsG0880045456.01.T01	MTR_4g093830	36.000	175	92	4	16	178	66	232	1.18e-20	87.8
MsG0880045456.01.T01	MTR_8g066780	33.108	148	91	2	4	143	48	195	6.02e-19	82.8
MsG0880045456.01.T01	MTR_5g030010	36.552	145	76	4	4	133	48	191	1.69e-18	81.6
MsG0880045456.01.T01	MTR_8g089880	53.846	65	26	1	69	133	136	196	1.79e-18	80.5
MsG0880045456.01.T01	MTR_7g113800	34.694	147	90	4	11	153	40	184	3.25e-18	80.9
MsG0880045456.01.T01	MTR_2g090905	39.080	87	51	1	53	137	32	118	4.21e-18	76.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045456.01.T01	AT5G17600	36.585	164	82	5	17	162	59	218	8.10e-20	85.5
MsG0880045456.01.T01	AT5G05280	34.127	126	72	2	11	132	37	155	7.75e-18	77.0
MsG0880045456.01.T01	AT1G35330	34.559	136	71	3	17	139	48	178	9.15e-18	79.3
MsG0880045456.01.T01	AT1G04360	29.861	144	79	5	13	136	39	180	1.46e-17	79.3
MsG0880045456.01.T01	AT3G03550	53.846	65	26	1	69	133	142	202	2.04e-17	78.6
MsG0880045456.01.T01	AT4G10160	46.053	76	39	1	90	163	97	172	5.39e-17	75.9
MsG0880045456.01.T01	AT3G18930	51.389	72	27	2	86	149	152	223	6.36e-17	77.8
MsG0880045456.01.T01	AT3G18930	51.389	72	27	2	86	149	152	223	6.36e-17	77.8
MsG0880045456.01.T01	AT3G18930	51.389	72	27	2	86	149	152	223	6.36e-17	77.8
MsG0880045456.01.T01	AT4G10150	44.737	76	40	1	90	163	111	186	7.99e-17	75.5

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Find 54 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCCCTCTTCAGTGCT+CGG	0.575081	8:+59347823	None:intergenic
AACACGCATCAGAACAATCG+CGG	0.689204	8:-59347873	MsG0880045456.01.T01:CDS
AATTAGGATCACACCAAATT+TGG	0.185152	8:+59347954	None:intergenic
ACAAACGCCGAGCACTGAAG+AGG	0.514908	8:-59347830	MsG0880045456.01.T01:CDS
ACACAGTTTATGGCTGATGA+TGG	0.638291	8:-59348000	None:intergenic
ACAGTGGGGAACCACCGGGG+AGG	0.631144	8:+59347582	None:intergenic
ACATATCAATGCATGGTACA+TGG	0.516171	8:+59347653	None:intergenic
ACCCAAAACGAGTTGCAAAA+TGG	0.276726	8:-59347493	MsG0880045456.01.T01:CDS
ACCGAGGTGAATGTTGATCA+TGG	0.552910	8:-59347541	MsG0880045456.01.T01:CDS
ACCGGAGGTTAGGTCCACCG+AGG	0.798348	8:-59347557	MsG0880045456.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTAGGATCACACCAAATT+TGG	+	Chr8:59347517-59347536	None:intergenic	30.0%
CCTTTGTTCAATACCAAATT+TGG	-	Chr8:59347501-59347520	MsG0880045456.01.T01:CDS	30.0%
TTTCTTCTTCTCATACTTGT+AGG	+	Chr8:59347691-59347710	None:intergenic	30.0%
ACATATCAATGCATGGTACA+TGG	+	Chr8:59347818-59347837	None:intergenic	35.0%
TGCAGCAAATGAATATGAGA+TGG	+	Chr8:59347575-59347594	None:intergenic	35.0%
TGTTGATCATGGCGTAAATA+CGG	-	Chr8:59347938-59347957	MsG0880045456.01.T01:CDS	35.0%
TTCTTCTCATACTTGTAGGA+TGG	+	Chr8:59347687-59347706	None:intergenic	35.0%
ACCCAAAACGAGTTGCAAAA+TGG	-	Chr8:59347975-59347994	MsG0880045456.01.T01:CDS	40.0%
CAGGACAATTGAGATGTGAA+AGG	+	Chr8:59347845-59347864	None:intergenic	40.0%
CCCAAAACGAGTTGCAAAAT+GGG	-	Chr8:59347976-59347995	MsG0880045456.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	59347477	59348013	59347477	ID=MsG0880045456.01;Name=MsG0880045456.01
Chr8	mRNA	59347477	59348013	59347477	ID=MsG0880045456.01.T01;Parent=MsG0880045456.01;Name=MsG0880045456.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|178
Chr8	exon	59347477	59348013	59347477	ID=MsG0880045456.01.T01:exon:3186;Parent=MsG0880045456.01.T01
Chr8	CDS	59347477	59348013	59347477	ID=MsG0880045456.01.T01:cds;Parent=MsG0880045456.01.T01
Chr8	mRNA	59347477	59347950	59347477	ID=MsG0880045456.01.T02;Parent=MsG0880045456.01;Name=MsG0880045456.01.T02;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|157
Chr8	exon	59347477	59347950	59347477	ID=MsG0880045456.01.T02:exon:3187;Parent=MsG0880045456.01.T02
Chr8	CDS	59347477	59347950	59347477	ID=MsG0880045456.01.T02:cds;Parent=MsG0880045456.01.T02
Chr8	mRNA	59347477	59347698	59347477	ID=MsG0880045456.01.T03;Parent=MsG0880045456.01;Name=MsG0880045456.01.T03;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|73
Chr8	exon	59347477	59347698	59347477	ID=MsG0880045456.01.T03:exon:3188;Parent=MsG0880045456.01.T03
Chr8	CDS	59347477	59347698	59347477	ID=MsG0880045456.01.T03:cds;Parent=MsG0880045456.01.T03

Gene Sequence

>MsG0880045456.01.T03

ATGCCTTCTTGCATGCACTCTTTCCATGTACCATGCATTGATATGTGGCTCCTTTCACATCTCAATTGTCCTGTTTGCCGTGCCGATGCCACGCCCTCCCCGGTGGTTCCCCACTGTATACCGGAGGTTAGGTCCACCGAGGTGAATGTTGATCATGGCGTAAATACGGTGCAGATAGTTGTCACCCAAAACGAGTTGCAAAATGGGTTACACGTGGTCTGA

>MsG0880045456.01.T02

ATGGGTTCAGCTAGCTTTATGGTTACAATATACCATCTCATATTCATTTGCTGCAAACACGCATCAGAACAATCGCGGCAGCATGAGCAACCGTTGACACAAACGCCGAGCACTGAAGAGGGAAGTTTGGTGTCACATCAGATTCCATCCTACAAGTATGAGAAGAAGAAAAAGAATGATGACAATGATGATGATTGTGTGACTTGTGCTGTGTGCTTAGGAGACTTTGAGGAAGGTGAGGAGTTGAGGACTATGCCTTCTTGCATGCACTCTTTCCATGTACCATGCATTGATATGTGGCTCCTTTCACATCTCAATTGTCCTGTTTGCCGTGCCGATGCCACGCCCTCCCCGGTGGTTCCCCACTGTATACCGGAGGTTAGGTCCACCGAGGTGAATGTTGATCATGGCGTAAATACGGTGCAGATAGTTGTCACCCAAAACGAGTTGCAAAATGGGTTACACGTGGTCTGA

>MsG0880045456.01.T01

ATGGCTGATGATGGTGATTATCAACCTTTGTTCAATACCAAATTTGGTGTGATCCTAATTGCCATGGGTTCAGCTAGCTTTATGGTTACAATATACCATCTCATATTCATTTGCTGCAAACACGCATCAGAACAATCGCGGCAGCATGAGCAACCGTTGACACAAACGCCGAGCACTGAAGAGGGAAGTTTGGTGTCACATCAGATTCCATCCTACAAGTATGAGAAGAAGAAAAAGAATGATGACAATGATGATGATTGTGTGACTTGTGCTGTGTGCTTAGGAGACTTTGAGGAAGGTGAGGAGTTGAGGACTATGCCTTCTTGCATGCACTCTTTCCATGTACCATGCATTGATATGTGGCTCCTTTCACATCTCAATTGTCCTGTTTGCCGTGCCGATGCCACGCCCTCCCCGGTGGTTCCCCACTGTATACCGGAGGTTAGGTCCACCGAGGTGAATGTTGATCATGGCGTAAATACGGTGCAGATAGTTGTCACCCAAAACGAGTTGCAAAATGGGTTACACGTGGTCTGA

Protein sequence

>MsG0880045456.01.T03

MPSCMHSFHVPCIDMWLLSHLNCPVCRADATPSPVVPHCIPEVRSTEVNVDHGVNTVQIVVTQNELQNGLHVV*

>MsG0880045456.01.T02

MGSASFMVTIYHLIFICCKHASEQSRQHEQPLTQTPSTEEGSLVSHQIPSYKYEKKKKNDDNDDDCVTCAVCLGDFEEGEELRTMPSCMHSFHVPCIDMWLLSHLNCPVCRADATPSPVVPHCIPEVRSTEVNVDHGVNTVQIVVTQNELQNGLHVV*

>MsG0880045456.01.T01

MADDGDYQPLFNTKFGVILIAMGSASFMVTIYHLIFICCKHASEQSRQHEQPLTQTPSTEEGSLVSHQIPSYKYEKKKKNDDNDDDCVTCAVCLGDFEEGEELRTMPSCMHSFHVPCIDMWLLSHLNCPVCRADATPSPVVPHCIPEVRSTEVNVDHGVNTVQIVVTQNELQNGLHVV*