AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045536.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045536.01.T01 MTR_8g073580 94.606 241 11 2 1 240 51 290 9.37e-153 426
MsG0880045536.01.T01 MTR_8g073580 94.828 232 10 2 10 240 13 243 7.71e-147 409
MsG0880045536.01.T01 MTR_3g061590 53.846 208 86 2 36 239 58 259 2.28e-73 224
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045536.01.T01 AT5G66010 67.623 244 64 3 1 240 23 255 2.63e-106 307
MsG0880045536.01.T01 AT5G66010 67.081 161 46 2 84 240 1 158 1.52e-64 198
MsG0880045536.01.T01 AT3G20890 42.979 235 103 2 36 239 58 292 3.33e-59 189
MsG0880045536.01.T01 AT3G20890 40.394 203 90 2 68 239 27 229 6.17e-44 148

Find 68 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCTCTCTTTGCCTCATC+TGG 0.312683 8:-60669678 None:intergenic
GAGGCCTTTGTAGTCTTTGC+AGG 0.327405 8:+60669297 MsG0880045536.01.T01:CDS
GATGCGTGGACTTCCTTTCT+TGG 0.329252 8:+60669536 MsG0880045536.01.T01:CDS
CCGCTGCTCACTGCGAAGTA+TGG 0.332843 8:-60669090 None:intergenic
CTAGCTTCATCTGGTGTAGA+AGG 0.341720 8:-60669747 None:intergenic
TCTCCTGTAGCTTTACCATC+TGG 0.351272 8:-60669633 None:intergenic
GACCATTGTCGATGTGTTAC+TGG 0.356325 8:+60669248 MsG0880045536.01.T01:CDS
ACAGGCAGCTATCAACATTA+TGG 0.356559 8:+60669114 MsG0880045536.01.T01:CDS
AACAAGAGTGGACGGTTCTC+TGG 0.381015 8:+60669273 MsG0880045536.01.T01:CDS
TTCTTTATCGCTGAACCTCT+TGG 0.382586 8:-60669489 None:intergenic
ATTACAAGCTGATAGAAGAT+AGG 0.386176 8:+60669592 MsG0880045536.01.T01:CDS
CTACACCAGATGAAGCTAGA+AGG 0.392023 8:+60669751 MsG0880045536.01.T01:CDS
GATTATTACAATGCTGTTGC+TGG 0.393585 8:+60669405 MsG0880045536.01.T01:CDS
TTCGCAGTGAGCAGCGGAAC+AGG 0.403956 8:+60669096 MsG0880045536.01.T01:CDS
TCGGCCCTTCTAGCTTCATC+TGG 0.450631 8:-60669756 None:intergenic
TCTATGTCTGTGCAGTTAAA+CGG 0.457816 8:-60669201 None:intergenic
CCTCTTGGACTGGAGGGAGG+AGG 0.458611 8:-60669474 None:intergenic
TATGGATATGCCGGTGGTGG+CGG 0.459791 8:+60669132 MsG0880045536.01.T01:CDS
CTCCTGTAGCTTTACCATCT+GGG 0.464237 8:-60669632 None:intergenic
TGAGATTTAGTCACCAAGAA+AGG 0.464772 8:-60669549 None:intergenic
GGGCTGGAAGCCGCCACCAC+CGG 0.468374 8:-60669142 None:intergenic
TGGAGGGAGGAGGGCTTCCT+TGG 0.472214 8:-60669464 None:intergenic
CGCCCAGATGGTAAAGCTAC+AGG 0.474461 8:+60669630 MsG0880045536.01.T01:CDS
CTTAGACGAACCACAGGAAA+AGG 0.474614 8:-60669171 None:intergenic
AGTCTTTGCAGGAGCAATGC+AGG 0.496210 8:+60669308 MsG0880045536.01.T01:CDS
TTCAAGGATAAAATGACCAT+TGG 0.498109 8:+60669702 MsG0880045536.01.T01:CDS
GAACCACAGGAAAAGGAGGA+GGG 0.503041 8:-60669164 None:intergenic
CTCTTGGACTGGAGGGAGGA+GGG 0.514245 8:-60669473 None:intergenic
GCTCACTGCGAAGTATGGAT+TGG 0.525157 8:-60669085 None:intergenic
ATAAAATGACCATTGGATCA+AGG 0.525711 8:+60669709 MsG0880045536.01.T01:CDS
GAACATGGGTCGCAGATATG+TGG 0.527311 8:+60669359 MsG0880045536.01.T01:CDS
GGCAAAGAGAGCAATGTTCA+AGG 0.531124 8:+60669686 MsG0880045536.01.T01:CDS
CGCTGAACCTCTTGGACTGG+AGG 0.532043 8:-60669481 None:intergenic
TATCGCTGAACCTCTTGGAC+TGG 0.532849 8:-60669484 None:intergenic
CATTATGGATATGCCGGTGG+TGG 0.541489 8:+60669129 MsG0880045536.01.T01:CDS
CACATTGCATGTCGCCCAGA+TGG 0.547640 8:+60669618 MsG0880045536.01.T01:CDS
CGAACCACAGGAAAAGGAGG+AGG 0.551442 8:-60669165 None:intergenic
GACCATTGGATCAAGGTATG+TGG 0.553651 8:+60669716 MsG0880045536.01.T01:CDS
TTTCCTGTGGTTCGTCTAAG+GGG 0.553773 8:+60669174 MsG0880045536.01.T01:CDS
ACAGAGAGATCGACAGAACA+TGG 0.556371 8:+60669344 MsG0880045536.01.T01:CDS
TTACAAGCTGATAGAAGATA+GGG 0.560052 8:+60669593 MsG0880045536.01.T01:CDS
TATCAACATTATGGATATGC+CGG 0.567709 8:+60669123 MsG0880045536.01.T01:CDS
AGAGTTTGTTTCGCCAGATG+AGG 0.577899 8:+60669665 MsG0880045536.01.T01:CDS
TACACCAGATGAAGCTAGAA+GGG 0.581811 8:+60669752 MsG0880045536.01.T01:CDS
GAAGGGCCGAGTCAAGATCA+AGG 0.583787 8:+60669769 MsG0880045536.01.T01:CDS
TTCCTGTGGTTCGTCTAAGG+GGG 0.588785 8:+60669175 MsG0880045536.01.T01:CDS
TTCCACATACCTTGATCCAA+TGG 0.592618 8:-60669718 None:intergenic
GCTGAACCTCTTGGACTGGA+GGG 0.594182 8:-60669480 None:intergenic
ACCACAGGAAAAGGAGGAGG+GGG 0.594356 8:-60669162 None:intergenic
TAGCTTCATCTGGTGTAGAA+GGG 0.595598 8:-60669746 None:intergenic
GAGTGGACGGTTCTCTGGAG+AGG 0.596938 8:+60669278 MsG0880045536.01.T01:CDS
GTGTTACTGGTCAACAAGAG+TGG 0.600765 8:+60669261 MsG0880045536.01.T01:CDS
AACCACAGGAAAAGGAGGAG+GGG 0.612802 8:-60669163 None:intergenic
CCTCCTCCCTCCAGTCCAAG+AGG 0.622146 8:+60669474 MsG0880045536.01.T01:CDS
GAACCTCTTGGACTGGAGGG+AGG 0.623273 8:-60669477 None:intergenic
CCATACTTCGCAGTGAGCAG+CGG 0.625249 8:+60669090 MsG0880045536.01.T01:CDS
TACTGGTCAACAAGAGTGGA+CGG 0.646908 8:+60669265 MsG0880045536.01.T01:CDS
GACCAGTAACACATCGACAA+TGG 0.647869 8:-60669250 None:intergenic
ACTGAGATATTGAAGATGCG+TGG 0.652021 8:+60669522 MsG0880045536.01.T01:CDS
TCACTGCCTTGATCTTGACT+CGG 0.652481 8:-60669775 None:intergenic
CAACATTATGGATATGCCGG+TGG 0.657238 8:+60669126 MsG0880045536.01.T01:CDS
AGACGAACCACAGGAAAAGG+AGG 0.657588 8:-60669168 None:intergenic
TGCTCCTGCAAAGACTACAA+AGG 0.672800 8:-60669301 None:intergenic
CAGAGAGATCGACAGAACAT+GGG 0.682980 8:+60669345 MsG0880045536.01.T01:CDS
AGATGGTAAAGCTACAGGAG+AGG 0.701989 8:+60669635 MsG0880045536.01.T01:CDS
ATATATGACAACGATTACCA+AGG 0.709272 8:+60669447 MsG0880045536.01.T01:CDS
GCTGGTGAGATCAATTACGA+AGG 0.716063 8:+60669423 MsG0880045536.01.T01:CDS
GAGTCAAGATCAAGGCAGTG+AGG 0.723826 8:+60669777 MsG0880045536.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAAAATGACCATTGGATCA+AGG + Chr8:60669709-60669728 MsG0880045536.01.T01:CDS 30.0%
ATATATGACAACGATTACCA+AGG + Chr8:60669447-60669466 MsG0880045536.01.T01:CDS 30.0%
ATTACAAGCTGATAGAAGAT+AGG + Chr8:60669592-60669611 MsG0880045536.01.T01:CDS 30.0%
TATCAACATTATGGATATGC+CGG + Chr8:60669123-60669142 MsG0880045536.01.T01:CDS 30.0%
TTACAAGCTGATAGAAGATA+GGG + Chr8:60669593-60669612 MsG0880045536.01.T01:CDS 30.0%
TTCAAGGATAAAATGACCAT+TGG + Chr8:60669702-60669721 MsG0880045536.01.T01:CDS 30.0%
GATTATTACAATGCTGTTGC+TGG + Chr8:60669405-60669424 MsG0880045536.01.T01:CDS 35.0%
TCTATGTCTGTGCAGTTAAA+CGG - Chr8:60669204-60669223 None:intergenic 35.0%
TGAGATTTAGTCACCAAGAA+AGG - Chr8:60669552-60669571 None:intergenic 35.0%
!!! GACATTTTGAAGTTCTTTGC+TGG + Chr8:60669222-60669241 MsG0880045536.01.T01:CDS 35.0%
ACAGGCAGCTATCAACATTA+TGG + Chr8:60669114-60669133 MsG0880045536.01.T01:CDS 40.0%
ACTGAGATATTGAAGATGCG+TGG + Chr8:60669522-60669541 MsG0880045536.01.T01:CDS 40.0%
TACACCAGATGAAGCTAGAA+GGG + Chr8:60669752-60669771 MsG0880045536.01.T01:CDS 40.0%
TAGCTTCATCTGGTGTAGAA+GGG - Chr8:60669749-60669768 None:intergenic 40.0%
TTCCACATACCTTGATCCAA+TGG - Chr8:60669721-60669740 None:intergenic 40.0%
TTCTTTATCGCTGAACCTCT+TGG - Chr8:60669492-60669511 None:intergenic 40.0%
! GCAGATATGTGGAAGTTTTC+AGG + Chr8:60669370-60669389 MsG0880045536.01.T01:CDS 40.0%
! TTTTCCTGTGGTTCGTCTAA+GGG + Chr8:60669173-60669192 MsG0880045536.01.T01:CDS 40.0%
!!! AGTTTTCAGGTGTAAGAAGC+AGG + Chr8:60669383-60669402 MsG0880045536.01.T01:CDS 40.0%
ACAGAGAGATCGACAGAACA+TGG + Chr8:60669344-60669363 MsG0880045536.01.T01:CDS 45.0%
AGATGGTAAAGCTACAGGAG+AGG + Chr8:60669635-60669654 MsG0880045536.01.T01:CDS 45.0%
ATTGCTCTCTTTGCCTCATC+TGG - Chr8:60669681-60669700 None:intergenic 45.0%
CAACATTATGGATATGCCGG+TGG + Chr8:60669126-60669145 MsG0880045536.01.T01:CDS 45.0%
CAGAGAGATCGACAGAACAT+GGG + Chr8:60669345-60669364 MsG0880045536.01.T01:CDS 45.0%
CTACACCAGATGAAGCTAGA+AGG + Chr8:60669751-60669770 MsG0880045536.01.T01:CDS 45.0%
CTAGCTTCATCTGGTGTAGA+AGG - Chr8:60669750-60669769 None:intergenic 45.0%
CTTAGACGAACCACAGGAAA+AGG - Chr8:60669174-60669193 None:intergenic 45.0%
GACCAGTAACACATCGACAA+TGG - Chr8:60669253-60669272 None:intergenic 45.0%
GACCATTGGATCAAGGTATG+TGG + Chr8:60669716-60669735 MsG0880045536.01.T01:CDS 45.0%
GACCATTGTCGATGTGTTAC+TGG + Chr8:60669248-60669267 MsG0880045536.01.T01:CDS 45.0%
GCTGGTGAGATCAATTACGA+AGG + Chr8:60669423-60669442 MsG0880045536.01.T01:CDS 45.0%
GGCAAAGAGAGCAATGTTCA+AGG + Chr8:60669686-60669705 MsG0880045536.01.T01:CDS 45.0%
TACTGGTCAACAAGAGTGGA+CGG + Chr8:60669265-60669284 MsG0880045536.01.T01:CDS 45.0%
TCACTGCCTTGATCTTGACT+CGG - Chr8:60669778-60669797 None:intergenic 45.0%
TGCTCCTGCAAAGACTACAA+AGG - Chr8:60669304-60669323 None:intergenic 45.0%
TTTCCTGTGGTTCGTCTAAG+GGG + Chr8:60669174-60669193 MsG0880045536.01.T01:CDS 45.0%
! AGAGTTTGTTTCGCCAGATG+AGG + Chr8:60669665-60669684 MsG0880045536.01.T01:CDS 45.0%
! CTCCTGTAGCTTTACCATCT+GGG - Chr8:60669635-60669654 None:intergenic 45.0%
! CTTTTCCTGTGGTTCGTCTA+AGG + Chr8:60669172-60669191 MsG0880045536.01.T01:CDS 45.0%
! GTGTTACTGGTCAACAAGAG+TGG + Chr8:60669261-60669280 MsG0880045536.01.T01:CDS 45.0%
! TCTCCTGTAGCTTTACCATC+TGG - Chr8:60669636-60669655 None:intergenic 45.0%
AACAAGAGTGGACGGTTCTC+TGG + Chr8:60669273-60669292 MsG0880045536.01.T01:CDS 50.0%
AACCACAGGAAAAGGAGGAG+GGG - Chr8:60669166-60669185 None:intergenic 50.0%
AGACGAACCACAGGAAAAGG+AGG - Chr8:60669171-60669190 None:intergenic 50.0%
AGTCTTTGCAGGAGCAATGC+AGG + Chr8:60669308-60669327 MsG0880045536.01.T01:CDS 50.0%
CATTATGGATATGCCGGTGG+TGG + Chr8:60669129-60669148 MsG0880045536.01.T01:CDS 50.0%
GAACATGGGTCGCAGATATG+TGG + Chr8:60669359-60669378 MsG0880045536.01.T01:CDS 50.0%
GAACCACAGGAAAAGGAGGA+GGG - Chr8:60669167-60669186 None:intergenic 50.0%
GAGGCCTTTGTAGTCTTTGC+AGG + Chr8:60669297-60669316 MsG0880045536.01.T01:CDS 50.0%
GATGCGTGGACTTCCTTTCT+TGG + Chr8:60669536-60669555 MsG0880045536.01.T01:CDS 50.0%
GCTCACTGCGAAGTATGGAT+TGG - Chr8:60669088-60669107 None:intergenic 50.0%
TATCGCTGAACCTCTTGGAC+TGG - Chr8:60669487-60669506 None:intergenic 50.0%
TTCCTGTGGTTCGTCTAAGG+GGG + Chr8:60669175-60669194 MsG0880045536.01.T01:CDS 50.0%
ACCACAGGAAAAGGAGGAGG+GGG - Chr8:60669165-60669184 None:intergenic 55.0%
CACATTGCATGTCGCCCAGA+TGG + Chr8:60669618-60669637 MsG0880045536.01.T01:CDS 55.0%
CCATACTTCGCAGTGAGCAG+CGG + Chr8:60669090-60669109 MsG0880045536.01.T01:CDS 55.0%
CGAACCACAGGAAAAGGAGG+AGG - Chr8:60669168-60669187 None:intergenic 55.0%
CGCCCAGATGGTAAAGCTAC+AGG + Chr8:60669630-60669649 MsG0880045536.01.T01:CDS 55.0%
GAAGGGCCGAGTCAAGATCA+AGG + Chr8:60669769-60669788 MsG0880045536.01.T01:CDS 55.0%
! GCTGAACCTCTTGGACTGGA+GGG - Chr8:60669483-60669502 None:intergenic 55.0%
! TATGGATATGCCGGTGGTGG+CGG + Chr8:60669132-60669151 MsG0880045536.01.T01:CDS 55.0%
!! TCGGCCCTTCTAGCTTCATC+TGG - Chr8:60669759-60669778 None:intergenic 55.0%
AGCCCCCTTAGACGAACCAC+AGG - Chr8:60669180-60669199 None:intergenic 60.0%
CCGCTGCTCACTGCGAAGTA+TGG - Chr8:60669093-60669112 None:intergenic 60.0%
TTCGCAGTGAGCAGCGGAAC+AGG + Chr8:60669096-60669115 MsG0880045536.01.T01:CDS 60.0%
! CGCTGAACCTCTTGGACTGG+AGG - Chr8:60669484-60669503 None:intergenic 60.0%
! CTCTTGGACTGGAGGGAGGA+GGG - Chr8:60669476-60669495 None:intergenic 60.0%
! GAACCTCTTGGACTGGAGGG+AGG - Chr8:60669480-60669499 None:intergenic 60.0%
! GAGTGGACGGTTCTCTGGAG+AGG + Chr8:60669278-60669297 MsG0880045536.01.T01:CDS 60.0%
CAGGAAAAGGAGGAGGGGGC+TGG - Chr8:60669161-60669180 None:intergenic 65.0%
CCTCCTCCCTCCAGTCCAAG+AGG + Chr8:60669474-60669493 MsG0880045536.01.T01:CDS 65.0%
TGGAGGGAGGAGGGCTTCCT+TGG - Chr8:60669467-60669486 None:intergenic 65.0%
! CCTCTTGGACTGGAGGGAGG+AGG - Chr8:60669477-60669496 None:intergenic 65.0%
! GCCCCCTCCTCCTTTTCCTG+TGG + Chr8:60669161-60669180 MsG0880045536.01.T01:CDS 65.0%
GGGCTGGAAGCCGCCACCAC+CGG - Chr8:60669145-60669164 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 60669075 60669797 60669075 ID=MsG0880045536.01;Name=MsG0880045536.01
Chr8 mRNA 60669075 60669797 60669075 ID=MsG0880045536.01.T01;Parent=MsG0880045536.01;Name=MsG0880045536.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|240
Chr8 exon 60669075 60669797 60669075 ID=MsG0880045536.01.T01:exon:23238;Parent=MsG0880045536.01.T01
Chr8 CDS 60669075 60669797 60669075 ID=MsG0880045536.01.T01:cds;Parent=MsG0880045536.01.T01
Gene Sequence

>MsG0880045536.01.T01

ATGATGGATTCCAATCCATACTTCGCAGTGAGCAGCGGAACAGGCAGCTATCAACATTATGGATATGCCGGTGGTGGCGGCTTCCAGCCCCCTCCTCCTTTTCCTGTGGTTCGTCTAAGGGGGCTTCCGTTTAACTGCACAGACATAGACATTTTGAAGTTCTTTGCTGGTCTGACCATTGTCGATGTGTTACTGGTCAACAAGAGTGGACGGTTCTCTGGAGAGGCCTTTGTAGTCTTTGCAGGAGCAATGCAGGTTGAGTTTGCTTTACAGAGAGATCGACAGAACATGGGTCGCAGATATGTGGAAGTTTTCAGGTGTAAGAAGCAGGATTATTACAATGCTGTTGCTGGTGAGATCAATTACGAAGGAATATATGACAACGATTACCAAGGAAGCCCTCCTCCCTCCAGTCCAAGAGGTTCAGCGATAAAGAACAAATGTTACACTGAGATATTGAAGATGCGTGGACTTCCTTTCTTGGTGACTAAATCTCAAATTATTGACTTTTTTAAAGATTACAAGCTGATAGAAGATAGGGTACACATTGCATGTCGCCCAGATGGTAAAGCTACAGGAGAGGCATATGTAGAGTTTGTTTCGCCAGATGAGGCAAAGAGAGCAATGTTCAAGGATAAAATGACCATTGGATCAAGGTATGTGGAACTGTTCCCTTCTACACCAGATGAAGCTAGAAGGGCCGAGTCAAGATCAAGGCAGTGA
Protein sequence

>MsG0880045536.01.T01

MMDSNPYFAVSSGTGSYQHYGYAGGGGFQPPPPFPVVRLRGLPFNCTDIDILKFFAGLTIVDVLLVNKSGRFSGEAFVVFAGAMQVEFALQRDRQNMGRRYVEVFRCKKQDYYNAVAGEINYEGIYDNDYQGSPPPSSPRGSAIKNKCYTEILKMRGLPFLVTKSQIIDFFKDYKLIEDRVHIACRPDGKATGEAYVEFVSPDEAKRAMFKDKMTIGSRYVELFPSTPDEARRAESRSRQ*