AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045781.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045781.01.T01 MTR_8g078900 85.714 133 5 2 1 132 1 120 4.70e-67 209
MsG0880045781.01.T01 MTR_8g078900 85.714 133 5 2 1 132 1 120 3.99e-65 209
MsG0880045781.01.T01 MTR_8g078900 94.444 90 4 1 44 132 1 90 1.26e-45 156
MsG0880045781.01.T01 MTR_8g078900 93.750 80 4 1 54 132 1 80 2.19e-38 134
MsG0880045781.01.T01 MTR_8g078900 93.750 80 4 1 54 132 1 80 3.32e-37 133
MsG0880045781.01.T01 MTR_2g048390 44.275 131 42 2 2 132 8 107 2.74e-29 110
MsG0880045781.01.T01 MTR_1g094850 44.262 122 37 4 11 132 17 107 1.97e-27 106
MsG0880045781.01.T01 MTR_2g018750 40.650 123 43 3 10 132 16 108 3.24e-23 94.4
MsG0880045781.01.T01 MTR_1g064360 41.667 108 46 2 25 132 18 108 3.45e-21 88.2
MsG0880045781.01.T01 MTR_2g009340 40.000 105 44 2 28 132 39 124 4.81e-21 88.2
MsG0880045781.01.T01 MTR_4g093850 36.190 105 48 1 28 132 39 124 5.99e-21 87.8
MsG0880045781.01.T01 MTR_4g093850 36.190 105 48 1 28 132 39 124 6.75e-21 87.4
MsG0880045781.01.T01 MTR_5g015790 43.925 107 40 3 26 132 20 106 8.70e-20 84.3
MsG0880045781.01.T01 MTR_2g084595 39.048 105 47 1 28 132 18 105 1.25e-19 83.6
MsG0880045781.01.T01 MTR_4g022330 40.000 100 41 1 33 132 34 114 1.41e-19 84.0
MsG0880045781.01.T01 MTR_1g106430 39.252 107 46 1 26 132 21 108 1.80e-19 83.6
MsG0880045781.01.T01 MTR_2g084595 39.048 105 47 1 28 132 18 105 1.85e-19 83.6
MsG0880045781.01.T01 MTR_4g095430 38.000 100 45 1 33 132 13 95 3.69e-19 82.4
MsG0880045781.01.T01 MTR_5g075640 39.604 101 42 1 33 133 25 106 5.73e-19 82.0
MsG0880045781.01.T01 MTR_3g062540 42.000 100 39 2 33 132 24 104 9.58e-18 78.6
MsG0880045781.01.T01 MTR_1g014070 37.037 108 49 2 25 132 19 107 1.06e-17 78.6
MsG0880045781.01.T01 MTR_5g016060 35.000 100 46 1 33 132 40 120 2.44e-17 77.4
MsG0880045781.01.T01 MTR_1g088885 37.500 96 41 1 35 130 38 114 3.14e-17 77.0
MsG0880045781.01.T01 MTR_5g032780 37.755 98 44 1 35 132 28 108 1.82e-16 75.1
MsG0880045781.01.T01 MTR_7g108290 35.417 96 43 1 35 130 38 114 1.11e-15 72.8
MsG0880045781.01.T01 MTR_1g082690 32.653 98 51 2 35 132 60 142 3.98e-15 70.9
MsG0880045781.01.T01 MTR_8g069330 37.113 97 43 2 36 132 29 107 1.69e-14 69.3
MsG0880045781.01.T01 MTR_7g073980 37.000 100 45 2 33 132 33 114 2.46e-14 68.9
MsG0880045781.01.T01 MTR_4g134210 33.981 103 49 1 30 132 23 106 6.95e-14 67.4
MsG0880045781.01.T01 MTR_2g027410 33.981 103 49 2 30 132 23 106 1.85e-13 66.2
MsG0880045781.01.T01 MTR_1g105870 33.333 96 47 2 37 132 35 113 1.17e-11 60.5
MsG0880045781.01.T01 MTR_5g075640 56.818 44 19 0 90 133 20 63 6.42e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045781.01.T01 AT5G66560 48.387 155 42 3 3 132 2 143 1.90e-38 135
MsG0880045781.01.T01 AT5G66560 47.742 155 43 3 3 132 2 143 1.43e-37 134
MsG0880045781.01.T01 AT1G30440 43.846 130 43 2 2 131 8 107 1.23e-29 112
MsG0880045781.01.T01 AT3G50840 46.400 125 37 2 8 132 3 97 1.61e-29 112
MsG0880045781.01.T01 AT5G03250 42.748 131 45 3 2 132 8 108 4.28e-25 99.8
MsG0880045781.01.T01 AT5G64330 38.211 123 44 3 10 132 42 132 5.08e-24 96.7
MsG0880045781.01.T01 AT5G64330 38.211 123 44 3 10 132 42 132 5.34e-24 96.7
MsG0880045781.01.T01 AT5G66560 44.737 114 38 2 44 132 1 114 3.91e-23 94.0
MsG0880045781.01.T01 AT5G67440 43.925 107 44 2 26 132 20 110 5.92e-22 90.5
MsG0880045781.01.T01 AT5G67440 43.925 107 44 2 26 132 20 110 5.92e-22 90.5
MsG0880045781.01.T01 AT5G13600 38.889 108 46 1 25 132 18 105 6.13e-21 87.8
MsG0880045781.01.T01 AT4G31820 38.889 108 47 2 25 132 19 107 1.63e-20 86.7
MsG0880045781.01.T01 AT3G26490 37.383 107 51 1 26 132 19 109 6.68e-19 82.0
MsG0880045781.01.T01 AT3G26490 37.383 107 51 1 26 132 19 109 7.10e-19 82.0
MsG0880045781.01.T01 AT3G26490 37.383 107 51 1 26 132 19 109 7.10e-19 82.0
MsG0880045781.01.T01 AT3G26490 37.383 107 51 1 26 132 19 109 7.10e-19 82.0
MsG0880045781.01.T01 AT4G37590 36.449 107 60 1 26 132 20 118 1.61e-18 80.9
MsG0880045781.01.T01 AT3G44820 38.095 105 49 2 28 132 18 106 2.07e-18 80.5
MsG0880045781.01.T01 AT5G67385 41.837 98 39 2 35 132 27 106 3.20e-18 80.1
MsG0880045781.01.T01 AT2G30520 39.000 100 42 1 33 132 30 110 4.52e-18 79.7
MsG0880045781.01.T01 AT5G48800 37.755 98 42 1 33 130 41 119 5.42e-18 79.3
MsG0880045781.01.T01 AT3G08570 36.000 100 47 1 33 132 34 116 1.72e-17 77.8
MsG0880045781.01.T01 AT2G47860 36.735 98 40 1 35 132 39 114 3.34e-17 77.0
MsG0880045781.01.T01 AT2G47860 34.951 103 45 1 30 132 34 114 4.58e-17 76.6
MsG0880045781.01.T01 AT1G03010 40.206 97 36 2 35 131 38 112 1.65e-16 75.1
MsG0880045781.01.T01 AT1G03010 40.206 97 36 2 35 131 11 85 1.72e-16 75.1
MsG0880045781.01.T01 AT5G10250 34.286 105 51 2 28 132 45 131 1.39e-15 72.4
MsG0880045781.01.T01 AT5G10250 34.286 105 51 2 28 132 45 131 1.39e-15 72.4
MsG0880045781.01.T01 AT3G08660 34.314 102 47 2 31 132 32 113 1.50e-15 72.4
MsG0880045781.01.T01 AT3G08660 35.000 100 45 2 33 132 34 113 1.58e-15 72.4
MsG0880045781.01.T01 AT1G03010 38.614 101 40 2 31 131 33 111 2.18e-15 72.0
MsG0880045781.01.T01 AT5G66560 39.423 104 38 2 54 132 1 104 7.10e-15 70.5
MsG0880045781.01.T01 AT2G23050 36.449 107 54 2 26 132 20 112 7.68e-15 70.1
MsG0880045781.01.T01 AT2G47860 34.343 99 64 1 35 132 39 137 1.67e-14 69.3
MsG0880045781.01.T01 AT2G47860 32.692 104 69 1 30 132 34 137 2.13e-14 68.9
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT1G67900 34.694 98 42 1 35 132 28 103 7.61e-14 67.4
MsG0880045781.01.T01 AT5G67440 40.449 89 37 2 44 132 1 73 7.88e-14 67.4
MsG0880045781.01.T01 AT2G14820 37.383 107 48 2 26 132 20 107 1.54e-13 66.6
MsG0880045781.01.T01 AT2G14820 37.383 107 48 2 26 132 20 107 1.54e-13 66.6
MsG0880045781.01.T01 AT5G64330 35.870 92 40 2 41 132 1 73 6.23e-13 64.7
MsG0880045781.01.T01 AT5G47800 30.392 102 52 1 31 132 24 106 1.05e-11 61.2
MsG0880045781.01.T01 AT5G47800 30.392 102 52 1 31 132 24 106 1.05e-11 61.2
MsG0880045781.01.T01 AT5G47800 30.392 102 52 1 31 132 24 106 1.05e-11 61.2
MsG0880045781.01.T01 AT3G50840 39.241 79 30 1 54 132 1 61 1.44e-11 60.8
MsG0880045781.01.T01 AT3G50840 39.241 79 30 1 54 132 1 61 1.44e-11 60.8
MsG0880045781.01.T01 AT5G67385 46.429 56 25 1 77 132 11 61 1.57e-11 60.8
MsG0880045781.01.T01 AT5G67385 46.429 56 25 1 77 132 11 61 1.57e-11 60.8
MsG0880045781.01.T01 AT5G67385 46.429 56 25 1 77 132 11 61 1.57e-11 60.8
MsG0880045781.01.T01 AT5G67385 46.429 56 25 1 77 132 11 61 1.57e-11 60.8
MsG0880045781.01.T01 AT5G47800 30.693 101 51 1 31 131 137 218 1.60e-11 60.8
MsG0880045781.01.T01 AT3G49970 34.783 92 40 2 41 132 2 73 1.99e-11 60.5
MsG0880045781.01.T01 AT3G49970 34.783 92 40 2 41 132 2 73 1.99e-11 60.5

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTCTTGTTCTTCTTGT+GGG 0.294335 8:+64316433 None:intergenic
ATTCTGCTACGGTGTTAAAA+TGG 0.302145 8:-64316331 MsG0880045781.01.T01:CDS
CCATGCTTGCCCTTTAGAGC+TGG 0.348806 8:+64316849 None:intergenic
CTCAGAGCCACCAGGGAAAT+CGG 0.381320 8:+64316374 None:intergenic
CATAAAGTTTAAAGAGAAGA+AGG 0.391186 8:+64316815 None:intergenic
TCAGAAAAGCCCAGCTCTAA+AGG 0.396326 8:-64316859 MsG0880045781.01.T01:CDS
CATGCTTGCCCTTTAGAGCT+GGG 0.415161 8:+64316850 None:intergenic
TCTTCGTCTTGTTCTTCTTG+TGG 0.438236 8:+64316432 None:intergenic
CAGAAAAGCCCAGCTCTAAA+GGG 0.451283 8:-64316858 MsG0880045781.01.T01:CDS
CTTTACCTTGTGAAGATGAA+AGG 0.510461 8:+64316655 None:intergenic
TGGCTCTGAGGCGTTCGAGA+TGG 0.540852 8:-64316361 MsG0880045781.01.T01:CDS
GTAGCTTCACTCCGTTGCGC+CGG 0.544567 8:-64316291 MsG0880045781.01.T01:CDS
GTAACATTCACCGATTTCCC+TGG 0.548638 8:-64316384 MsG0880045781.01.T01:CDS
CTCAAGAAACACCGGCGCAA+CGG 0.549272 8:+64316280 None:intergenic
CCAGCTCTAAAGGGCAAGCA+TGG 0.572012 8:-64316849 MsG0880045781.01.T01:CDS
CGGAGTGAAGCTACATTGGA+AGG 0.575593 8:+64316300 None:intergenic
CGATTTCCCTGGTGGCTCTG+AGG 0.578786 8:-64316373 MsG0880045781.01.T01:CDS
GCAACGGAGTGAAGCTACAT+TGG 0.591099 8:+64316296 None:intergenic
TCGAACGCCTCAGAGCCACC+AGG 0.593922 8:+64316366 None:intergenic
CTCTGAGGCGTTCGAGATGG+CGG 0.599023 8:-64316358 MsG0880045781.01.T01:CDS
ACATTCACCGATTTCCCTGG+TGG 0.610000 8:-64316381 MsG0880045781.01.T01:CDS
ACTTCAACAACAATGTCACT+TGG 0.618807 8:+64316689 None:intergenic
ATGGCGGCTAAATTCTGCTA+CGG 0.632845 8:-64316342 MsG0880045781.01.T01:CDS
GTCAAGAACTCAAGAAACAC+CGG 0.632994 8:+64316272 None:intergenic
CGAACGCCTCAGAGCCACCA+GGG 0.667628 8:+64316367 None:intergenic
TATGACCTTTCATCTTCACA+AGG 0.678388 8:-64316660 MsG0880045781.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTGTAGTTACTATAAT+TGG + Chr8:64316575-64316594 None:intergenic 20.0%
! AAACACACACAAAAAAAACT+TGG - Chr8:64316541-64316560 MsG0880045781.01.T01:intron 25.0%
! AACACACACAAAAAAAACTT+GGG - Chr8:64316542-64316561 MsG0880045781.01.T01:intron 25.0%
! CATAAAGTTTAAAGAGAAGA+AGG + Chr8:64316337-64316356 None:intergenic 25.0%
! GAAACTGGAAGAAAAAAAAT+GGG + Chr8:64316620-64316639 None:intergenic 25.0%
!!! CTTCTTCTTGTTGTTTTATT+AGG + Chr8:64316674-64316693 None:intergenic 25.0%
!!! TTTCTACTTTTTGACATAAG+TGG + Chr8:64316642-64316661 None:intergenic 25.0%
GGAAACTGGAAGAAAAAAAA+TGG + Chr8:64316621-64316640 None:intergenic 30.0%
! TTTTTGACATAAGTGGAAAC+TGG + Chr8:64316635-64316654 None:intergenic 30.0%
ACTTCAACAACAATGTCACT+TGG + Chr8:64316463-64316482 None:intergenic 35.0%
CAATCATAATTCCACTATGC+AGG - Chr8:64316419-64316438 MsG0880045781.01.T01:CDS 35.0%
CTTTACCTTGTGAAGATGAA+AGG + Chr8:64316497-64316516 None:intergenic 35.0%
TATGACCTTTCATCTTCACA+AGG - Chr8:64316489-64316508 MsG0880045781.01.T01:CDS 35.0%
! ATTCTGCTACGGTGTTAAAA+TGG - Chr8:64316818-64316837 MsG0880045781.01.T01:CDS 35.0%
GTTGTACAAAACCTGCATAG+TGG + Chr8:64316433-64316452 None:intergenic 40.0%
! CTTCGTCTTGTTCTTCTTGT+GGG + Chr8:64316719-64316738 None:intergenic 40.0%
! TCTTCGTCTTGTTCTTCTTG+TGG + Chr8:64316720-64316739 None:intergenic 40.0%
!! CTATGCAGGTTTTGTACAAC+TGG - Chr8:64316433-64316452 MsG0880045781.01.T01:CDS 40.0%
ATGGCGGCTAAATTCTGCTA+CGG - Chr8:64316807-64316826 MsG0880045781.01.T01:intron 45.0%
! TCAGAAAAGCCCAGCTCTAA+AGG - Chr8:64316290-64316309 MsG0880045781.01.T01:CDS 45.0%
!! CAGAAAAGCCCAGCTCTAAA+GGG - Chr8:64316291-64316310 MsG0880045781.01.T01:CDS 45.0%
!! GTAACATTCACCGATTTCCC+TGG - Chr8:64316765-64316784 MsG0880045781.01.T01:intron 45.0%
CGGAGTGAAGCTACATTGGA+AGG + Chr8:64316852-64316871 None:intergenic 50.0%
GCAACGGAGTGAAGCTACAT+TGG + Chr8:64316856-64316875 None:intergenic 50.0%
!! ACATTCACCGATTTCCCTGG+TGG - Chr8:64316768-64316787 MsG0880045781.01.T01:intron 50.0%
!! CATGCTTGCCCTTTAGAGCT+GGG + Chr8:64316302-64316321 None:intergenic 50.0%
CTCAGAGCCACCAGGGAAAT+CGG + Chr8:64316778-64316797 None:intergenic 55.0%
!! CCAGCTCTAAAGGGCAAGCA+TGG - Chr8:64316300-64316319 MsG0880045781.01.T01:CDS 55.0%
!! CCATGCTTGCCCTTTAGAGC+TGG + Chr8:64316303-64316322 None:intergenic 55.0%
GTAGCTTCACTCCGTTGCGC+CGG - Chr8:64316858-64316877 MsG0880045781.01.T01:CDS 60.0%
! CGATTTCCCTGGTGGCTCTG+AGG - Chr8:64316776-64316795 MsG0880045781.01.T01:intron 60.0%
!! CTCTGAGGCGTTCGAGATGG+CGG - Chr8:64316791-64316810 MsG0880045781.01.T01:intron 60.0%
!! TGGCTCTGAGGCGTTCGAGA+TGG - Chr8:64316788-64316807 MsG0880045781.01.T01:intron 60.0%
CGAACGCCTCAGAGCCACCA+GGG + Chr8:64316785-64316804 None:intergenic 65.0%
TCGAACGCCTCAGAGCCACC+AGG + Chr8:64316786-64316805 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 64316281 64316890 64316281 ID=MsG0880045781.01;Name=MsG0880045781.01
Chr8 mRNA 64316281 64316890 64316281 ID=MsG0880045781.01.T01;Parent=MsG0880045781.01;Name=MsG0880045781.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0.5|0.66|3|0|134
Chr8 exon 64316811 64316890 64316811 ID=MsG0880045781.01.T01:exon:27109;Parent=MsG0880045781.01.T01
Chr8 exon 64316661 64316730 64316661 ID=MsG0880045781.01.T01:exon:27108;Parent=MsG0880045781.01.T01
Chr8 exon 64316281 64316535 64316281 ID=MsG0880045781.01.T01:exon:27107;Parent=MsG0880045781.01.T01
Chr8 CDS 64316811 64316890 64316811 ID=MsG0880045781.01.T01:cds;Parent=MsG0880045781.01.T01
Chr8 CDS 64316661 64316730 64316661 ID=MsG0880045781.01.T01:cds;Parent=MsG0880045781.01.T01
Chr8 CDS 64316281 64316535 64316281 ID=MsG0880045781.01.T01:cds;Parent=MsG0880045781.01.T01
Gene Sequence

>MsG0880045781.01.T01

ATGTCATCTTCAGAAAAGCCCAGCTCTAAAGGGCAAGCATGGTTAGATAACAACCTTCTTCTCTTTAAACTTTATGTTATGTTTTGTACAACTGGACTACCAAGTGACATTGTTGTTGAAGTAGATGATATGACCTTTCATCTTCACAAGTTTCCACTTATGTCAAAAAGTAGAAAGCTTCACAACCTAATAAAACAACAAGAAGAAGAAGCAATAACACATTCTACAATCCCACAAGAAGAACAAGACGAAGACGAAATCGTTGAAGAACAATGTCATGTAACATTCACCGATTTCCCTGGTGGCTCTGAGGCGTTCGAGATGGCGGCTAAATTCTGCTACGGTGTTAAAATGGAACTGTCACCTTCCAATGTAGCTTCACTCCGTTGCGCCGGTGTTTCTTGA
Protein sequence

>MsG0880045781.01.T01

MSSSEKPSSKGQAWLDNNLLLFKLYVMFCTTGLPSDIVVEVDDMTFHLHKFPLMSKSRKLHNLIKQQEEEAITHSTIPQEEQDEDEIVEEQCHVTFTDFPGGSEAFEMAAKFCYGVKMELSPSNVASLRCAGVS*