AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046309.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046309.01.T01 MTR_4g105310 89.286 84 7 1 1 84 86 167 1.76e-46 151
MsG0880046309.01.T01 MTR_4g105310 89.286 84 7 1 1 84 86 167 1.60e-45 151
MsG0880046309.01.T01 MTR_4g105310 91.525 59 5 0 74 132 266 324 4.38e-31 114
MsG0880046309.01.T01 MTR_3g077410 75.000 84 20 1 1 84 75 157 1.83e-36 127
MsG0880046309.01.T01 MTR_3g077410 79.661 59 12 0 74 132 255 313 1.60e-26 101
MsG0880046309.01.T01 MTR_6g053600 50.000 90 44 1 3 92 63 151 2.51e-20 84.3
MsG0880046309.01.T01 MTR_6g053550 48.889 90 45 1 3 92 77 165 1.59e-19 82.8
MsG0880046309.01.T01 MTR_8g098415 46.067 89 47 1 1 89 67 154 8.50e-19 79.0
MsG0880046309.01.T01 MTR_1g100315 47.059 102 46 2 4 105 70 163 8.92e-19 80.5
MsG0880046309.01.T01 MTR_1g100395 44.737 114 51 3 3 112 69 174 9.29e-19 80.5
MsG0880046309.01.T01 MTR_7g099850 47.253 91 47 1 4 94 75 164 1.77e-18 79.7
MsG0880046309.01.T01 MTR_8g098415 46.067 89 47 1 1 89 67 154 2.24e-18 79.3
MsG0880046309.01.T01 MTR_8g098415 46.067 89 47 1 1 89 10 97 2.38e-18 78.6
MsG0880046309.01.T01 MTR_8g098415 46.067 89 47 1 1 89 67 154 2.55e-18 79.3
MsG0880046309.01.T01 MTR_1g100335 48.315 89 45 1 3 91 69 156 6.22e-18 77.4
MsG0880046309.01.T01 MTR_1g100335 48.315 89 45 1 3 91 69 156 7.18e-18 77.4
MsG0880046309.01.T01 MTR_1g100335 48.315 89 45 1 3 91 69 156 7.68e-18 77.8
MsG0880046309.01.T01 MTR_8g099995 44.444 81 43 1 4 84 76 154 2.17e-17 77.0
MsG0880046309.01.T01 MTR_8g099995 59.615 52 21 0 74 125 255 306 1.75e-14 68.9
MsG0880046309.01.T01 MTR_1g068970 43.956 91 50 1 4 94 8 97 1.13e-16 73.9
MsG0880046309.01.T01 MTR_1g068970 43.956 91 50 1 4 94 75 164 2.37e-16 73.9
MsG0880046309.01.T01 MTR_1g100305 44.828 87 47 1 3 89 69 154 4.48e-16 73.2
MsG0880046309.01.T01 MTR_8g022500 57.377 61 25 1 22 81 459 519 5.72e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046309.01.T01 AT5G50130 76.389 72 17 0 3 74 76 147 2.08e-32 115
MsG0880046309.01.T01 AT5G50130 76.389 72 17 0 3 74 76 147 2.10e-32 115
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 43 113 1.29e-31 114
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 43 113 1.34e-31 114
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 76 146 1.57e-31 114
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 76 146 1.65e-31 114
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 43 113 1.94e-31 114
MsG0880046309.01.T01 AT5G50130 74.545 55 14 0 72 126 220 274 6.17e-23 91.7
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 43 113 1.94e-31 114
MsG0880046309.01.T01 AT5G50130 74.545 55 14 0 72 126 219 273 6.02e-23 91.7
MsG0880046309.01.T01 AT5G50130 77.465 71 16 0 3 73 76 146 2.25e-31 114
MsG0880046309.01.T01 AT5G50130 74.545 55 14 0 72 126 253 307 8.89e-23 91.7
MsG0880046309.01.T01 AT4G24050 43.363 113 64 0 4 116 75 187 7.61e-24 93.6
MsG0880046309.01.T01 AT4G24050 43.636 110 62 0 4 113 75 184 2.16e-23 93.2
MsG0880046309.01.T01 AT4G24050 54.545 55 25 0 74 128 254 308 5.97e-14 67.4
MsG0880046309.01.T01 AT1G64590 58.571 70 29 0 4 73 75 144 2.28e-22 90.5
MsG0880046309.01.T01 AT1G64590 54.167 48 22 0 74 121 254 301 4.96e-12 62.0
MsG0880046309.01.T01 AT4G11410 46.154 91 48 1 2 92 68 157 1.51e-19 82.0
MsG0880046309.01.T01 AT4G11410 46.154 91 48 1 2 92 68 157 2.47e-19 82.0
MsG0880046309.01.T01 AT4G23430 49.333 75 38 0 2 76 68 142 9.25e-19 80.5
MsG0880046309.01.T01 AT4G23430 49.333 75 38 0 2 76 68 142 1.01e-18 80.5
MsG0880046309.01.T01 AT4G23430 48.193 83 41 1 2 84 68 148 3.73e-18 79.0
MsG0880046309.01.T01 AT4G23430 48.193 83 41 1 2 84 68 148 3.91e-18 79.0
MsG0880046309.01.T01 AT4G23420 48.193 83 41 1 2 84 68 148 4.90e-18 77.8
MsG0880046309.01.T01 AT4G23420 48.193 83 41 1 2 84 68 148 6.61e-18 77.4
MsG0880046309.01.T01 AT4G23420 48.193 83 41 1 2 84 68 148 9.36e-18 77.8
MsG0880046309.01.T01 AT4G23420 48.193 83 41 1 2 84 68 148 9.36e-18 77.8
MsG0880046309.01.T01 AT4G23420 48.193 83 41 1 2 84 85 165 1.00e-17 77.8
MsG0880046309.01.T01 AT5G02540 41.176 119 56 3 4 122 74 178 1.85e-17 77.0
MsG0880046309.01.T01 AT5G02540 48.333 60 31 0 76 135 252 311 1.19e-12 63.9
MsG0880046309.01.T01 AT2G37540 48.148 81 40 1 4 84 74 152 3.29e-16 73.2
MsG0880046309.01.T01 AT2G37540 48.148 81 40 1 4 84 74 152 6.56e-16 72.8

Find 48 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAATTTCTTTCATTTAA+TGG 0.081513 8:+71820466 MsG0880046309.01.T01:three_prime_UTR
CATTGCAGGCCTAGTGGTTT+TGG 0.199593 8:+71816725 MsG0880046309.01.T01:five_prime_UTR
ATTTATTGTATAATATAAAA+TGG 0.269308 8:+71820513 MsG0880046309.01.T01:three_prime_UTR
ACACTTACATGAGAATATTA+AGG 0.272671 8:-71817245 None:intergenic
TTGTATTGTTCCATAGCTTT+TGG 0.280623 8:-71820280 None:intergenic
TTCAATTGAGGTGCTACTTC+TGG 0.288572 8:+71817019 MsG0880046309.01.T01:intron
TGAAGGAACAATAAGGTATT+TGG 0.288975 8:+71816698 MsG0880046309.01.T01:five_prime_UTR
TGGTTGGCAACAAAAGAAAA+AGG 0.290146 8:+71816665 MsG0880046309.01.T01:five_prime_UTR
GTAAGACTCTTTAGTTATAT+AGG 0.316450 8:+71820577 MsG0880046309.01.T01:three_prime_UTR
AGGTATTTGGCAGGCATTGC+AGG 0.335192 8:+71816711 MsG0880046309.01.T01:five_prime_UTR
CCATTAAATGAAAGAAATTA+AGG 0.337870 8:-71820466 None:intergenic
TTATGCTGAGGTTATTCTAT+TGG 0.355615 8:+71817155 MsG0880046309.01.T01:CDS
ATGAGAGCTGTTAGGTTTGA+AGG 0.360069 8:-71816797 None:intergenic
TGAGGTGCTACTTCTGGTAT+TGG 0.371917 8:+71817025 MsG0880046309.01.T01:intron
TGAGAGTTGTGGTTGGTGCC+AGG 0.429651 8:+71817083 MsG0880046309.01.T01:five_prime_UTR
CAGCATAAGGACTCTCCTCT+TGG 0.434439 8:-71817140 None:intergenic
AAACTACAATATTATAATCA+AGG 0.450670 8:+71820419 MsG0880046309.01.T01:three_prime_UTR
ACATTTGCTACAAATTACTT+AGG 0.455021 8:+71817625 MsG0880046309.01.T01:CDS
TCATCGATGAAGGAACAATA+AGG 0.457856 8:+71816691 MsG0880046309.01.T01:five_prime_UTR
CACTTACATGAGAATATTAA+GGG 0.464360 8:-71817244 None:intergenic
AATAGAATAACCTCAGCATA+AGG 0.472498 8:-71817153 None:intergenic
GAATTTCCCACTCACCCCTT+CGG 0.485875 8:-71820203 None:intergenic
TACCAGTGATGAGAGCTGTT+AGG 0.489625 8:-71816805 None:intergenic
AACTACAATATTATAATCAA+GGG 0.503959 8:+71820420 MsG0880046309.01.T01:three_prime_UTR
TGAGAGCTGTTAGGTTTGAA+GGG 0.521862 8:-71816796 None:intergenic
CCAGAGGAGATGAGAGAGGA+AGG 0.522918 8:-71820663 None:intergenic
AGATACATGGTCTAGAATAG+AGG 0.530017 8:-71820350 None:intergenic
TTGGTGCCAGGGACATGAAA+AGG 0.532553 8:+71817095 MsG0880046309.01.T01:five_prime_UTR
AACCTAACAGCTCTCATCAC+TGG 0.543509 8:+71816803 MsG0880046309.01.T01:five_prime_UTR
CGAATCGAAGCCAAAAGCTA+TGG 0.554763 8:+71820270 MsG0880046309.01.T01:CDS
ATATTATAATCAAGGGGAAG+AGG 0.555815 8:+71820427 MsG0880046309.01.T01:three_prime_UTR
GAGAGTTGTGGTTGGTGCCA+GGG 0.563988 8:+71817084 MsG0880046309.01.T01:five_prime_UTR
AACGACTTAGACAAGCAGCT+TGG 0.564125 8:+71820315 MsG0880046309.01.T01:three_prime_UTR
GAATTTGAGCCAAAACCACT+AGG 0.567394 8:-71816734 None:intergenic
GGCAGGCATTGCAGGCCTAG+TGG 0.576133 8:+71816719 MsG0880046309.01.T01:five_prime_UTR
GATACATGGTCTAGAATAGA+GGG 0.578220 8:-71820349 None:intergenic
GAGCTGTTAGGTTTGAAGGG+AGG 0.584163 8:-71816793 None:intergenic
GGAACAATAAGGTATTTGGC+AGG 0.592376 8:+71816702 MsG0880046309.01.T01:five_prime_UTR
AACTAGTTGGAGGACACGAA+TGG 0.600276 8:+71816645 None:intergenic
TATACACACACATGCATGCA+CGG 0.603638 8:-71820378 None:intergenic
AGTTGGAGGACACGAATGGT+TGG 0.604406 8:+71816649 None:intergenic
GCTGCTCAAAACGATATCAC+AGG 0.618153 8:+71820028 MsG0880046309.01.T01:CDS
AGGAGTGTACTCTCATAACC+TGG 0.628213 8:+71817576 MsG0880046309.01.T01:CDS
ATGCACGGTGTCTAGATACA+TGG 0.629660 8:-71820363 None:intergenic
AGAGGAGTGAGAGTTGTGGT+TGG 0.631623 8:+71817076 MsG0880046309.01.T01:five_prime_UTR
ACTACAATATTATAATCAAG+GGG 0.658253 8:+71820421 MsG0880046309.01.T01:three_prime_UTR
AGAGGAGAGTCCTTATGCTG+AGG 0.678257 8:+71817143 MsG0880046309.01.T01:CDS
ACTTACATGAGAATATTAAG+GGG 0.701725 8:-71817243 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAATATTTTTTTGAATAT+TGG + Chr8:71819505-71819524 MsG0880046309.01.T01:intron 10.0%
!!! ATGAAATTTTTGAAAATATA+TGG - Chr8:71819979-71819998 None:intergenic 10.0%
!!! CTAATATTTTTTTGAATATT+GGG + Chr8:71819506-71819525 MsG0880046309.01.T01:intron 10.0%
!!! TACATTTTTGAATATTATTT+TGG - Chr8:71818270-71818289 None:intergenic 10.0%
!! AAACTACAATATTATAATCA+AGG + Chr8:71820419-71820438 MsG0880046309.01.T01:three_prime_UTR 15.0%
!! AACTACAATATTATAATCAA+GGG + Chr8:71820420-71820439 MsG0880046309.01.T01:three_prime_UTR 15.0%
!! ACAATATAAAACTAAAGTAA+TGG + Chr8:71818569-71818588 MsG0880046309.01.T01:intron 15.0%
!!! AATGAATATATGTTTTGTTT+TGG + Chr8:71816992-71817011 MsG0880046309.01.T01:intron 15.0%
!!! ATTGTTTCAAACAATTTTAT+AGG + Chr8:71819938-71819957 MsG0880046309.01.T01:intron 15.0%
!!! TACAAAGAGAAATATTTTAA+CGG - Chr8:71818058-71818077 None:intergenic 15.0%
!!! TCTATGCATTAATTAAATTT+TGG + Chr8:71819277-71819296 MsG0880046309.01.T01:intron 15.0%
!!! TTTACTTTTGTTTGAATATT+GGG + Chr8:71819789-71819808 MsG0880046309.01.T01:intron 15.0%
!!! TTTTACTTTTGTTTGAATAT+TGG + Chr8:71819788-71819807 MsG0880046309.01.T01:intron 15.0%
!! AAAATATTAAGCAATTTGAG+AGG - Chr8:71819722-71819741 None:intergenic 20.0%
!! AACAAAAATGTAGTTACAAT+GGG + Chr8:71819320-71819339 MsG0880046309.01.T01:intron 20.0%
!! ACTACAATATTATAATCAAG+GGG + Chr8:71820421-71820440 MsG0880046309.01.T01:three_prime_UTR 20.0%
!! CCATTAAATGAAAGAAATTA+AGG - Chr8:71820469-71820488 None:intergenic 20.0%
!! CCTTAATTTCTTTCATTTAA+TGG + Chr8:71820466-71820485 MsG0880046309.01.T01:three_prime_UTR 20.0%
!! GAATATTGGGAAATAATTAT+AGG + Chr8:71819519-71819538 MsG0880046309.01.T01:intron 20.0%
!! GTCATATCATATATGATTAA+GGG + Chr8:71819460-71819479 MsG0880046309.01.T01:intron 20.0%
!! TAACAAAAATGTAGTTACAA+TGG + Chr8:71819319-71819338 MsG0880046309.01.T01:intron 20.0%
!! TGTCATATCATATATGATTA+AGG + Chr8:71819459-71819478 MsG0880046309.01.T01:intron 20.0%
!! TTGAAAATATATGGCTTAAT+TGG - Chr8:71819970-71819989 None:intergenic 20.0%
!!! AAACATTTTGAAAGCTAATT+TGG + Chr8:71818900-71818919 MsG0880046309.01.T01:intron 20.0%
!!! AATTTGTTTGAATTAGAAGA+AGG + Chr8:71818962-71818981 MsG0880046309.01.T01:intron 20.0%
!!! CTAATTTTTACTATACTTCA+TGG - Chr8:71818450-71818469 None:intergenic 20.0%
!!! GTTCTATGCTTAATTAATTT+TGG + Chr8:71819572-71819591 MsG0880046309.01.T01:intron 20.0%
! AAATATTAAGGCAATTTGAG+AGG - Chr8:71819433-71819452 None:intergenic 25.0%
! AAGCATAAGAAATGACAATA+AGG - Chr8:71817290-71817309 None:intergenic 25.0%
! ACACAATATAATCACAATTG+AGG - Chr8:71817416-71817435 None:intergenic 25.0%
! ACACTTACATGAGAATATTA+AGG - Chr8:71817248-71817267 None:intergenic 25.0%
! ACTTACATGAGAATATTAAG+GGG - Chr8:71817246-71817265 None:intergenic 25.0%
! ATACAAAAATGTAGTGTACA+TGG + Chr8:71819612-71819631 MsG0880046309.01.T01:intron 25.0%
! ATGACAAGAGCAAAATATTA+AGG - Chr8:71819445-71819464 None:intergenic 25.0%
! ATTCGTAAAATATAACGAGT+TGG + Chr8:71818692-71818711 MsG0880046309.01.T01:intron 25.0%
! ATTGTGATTATATTGTGTTG+TGG + Chr8:71817418-71817437 MsG0880046309.01.T01:intron 25.0%
! CACTTACATGAGAATATTAA+GGG - Chr8:71817247-71817266 None:intergenic 25.0%
! CTTTGATGCAATAAAAAACA+AGG - Chr8:71820009-71820028 None:intergenic 25.0%
! GAAGATAAGTTCAATATGTT+AGG - Chr8:71817963-71817982 None:intergenic 25.0%
! GTAAGACTCTTTAGTTATAT+AGG + Chr8:71820577-71820596 MsG0880046309.01.T01:three_prime_UTR 25.0%
! TCATGAAAAATTGAGAATGT+TGG + Chr8:71818381-71818400 MsG0880046309.01.T01:intron 25.0%
! TGCAAGTATATACATAATAC+TGG - Chr8:71816940-71816959 None:intergenic 25.0%
! TGTCTTTAACACATGTTTAA+GGG - Chr8:71818234-71818253 None:intergenic 25.0%
! TTAATAAAAGTAAAGACACG+CGG - Chr8:71818008-71818027 None:intergenic 25.0%
! TTCGTAAAATATAACGAGTT+GGG + Chr8:71818693-71818712 MsG0880046309.01.T01:intron 25.0%
! TTTATGTATATCGGACTAAA+CGG - Chr8:71818643-71818662 None:intergenic 25.0%
! TTTGTTAACAAAAATGCTAC+TGG + Chr8:71819085-71819104 MsG0880046309.01.T01:intron 25.0%
!! AACTCGTTATATTTTACGAA+TGG - Chr8:71818693-71818712 None:intergenic 25.0%
!! AATGCATTTTGTCTTTGTTA+AGG - Chr8:71817746-71817765 None:intergenic 25.0%
!! ACATTTGCTACAAATTACTT+AGG + Chr8:71817625-71817644 MsG0880046309.01.T01:CDS 25.0%
!! ATGCATTTTGTCTTTGTTAA+GGG - Chr8:71817745-71817764 None:intergenic 25.0%
!! ATTCAGGATTTTCAGTTAAT+GGG + Chr8:71817820-71817839 MsG0880046309.01.T01:intron 25.0%
!! CAAATCTAAAGCTAAAAACT+CGG - Chr8:71817223-71817242 None:intergenic 25.0%
!! TGTCAATTCAATTTTCTCTT+CGG - Chr8:71817608-71817627 None:intergenic 25.0%
!! TTAGCTTTCAAAATGTTTCA+TGG - Chr8:71818898-71818917 None:intergenic 25.0%
!! TTCTTAATTTTGCAGAGAAA+AGG - Chr8:71819252-71819271 None:intergenic 25.0%
!! TTTTCATTCTTACATATGAG+AGG + Chr8:71817653-71817672 MsG0880046309.01.T01:intron 25.0%
!!! TGTTTTGGAATTTTCAATTG+AGG + Chr8:71817007-71817026 MsG0880046309.01.T01:intron 25.0%
AAAACTAAAGTAATGGTGCA+TGG + Chr8:71818576-71818595 MsG0880046309.01.T01:intron 30.0%
AATAGAATAACCTCAGCATA+AGG - Chr8:71817156-71817175 None:intergenic 30.0%
AATATGTATTTGCACTTTGC+AGG + Chr8:71820141-71820160 MsG0880046309.01.T01:intron 30.0%
AATGTACCTACCTAATTAGA+AGG - Chr8:71818544-71818563 None:intergenic 30.0%
AGAATTATTAGAGCACACAA+GGG + Chr8:71819876-71819895 MsG0880046309.01.T01:intron 30.0%
AGTGAAAGAAAAAATCCAAG+AGG + Chr8:71817125-71817144 MsG0880046309.01.T01:CDS 30.0%
ATATGTATTTGCACTTTGCA+GGG + Chr8:71820142-71820161 MsG0880046309.01.T01:intron 30.0%
ATATTATAATCAAGGGGAAG+AGG + Chr8:71820427-71820446 MsG0880046309.01.T01:three_prime_UTR 30.0%
ATCATTAATCTCTCCAACTA+AGG - Chr8:71820095-71820114 None:intergenic 30.0%
GAAAAATTGAGAATGTTGGT+GGG + Chr8:71818385-71818404 MsG0880046309.01.T01:intron 30.0%
GATAGATACTGCAAATAAAC+AGG + Chr8:71819114-71819133 MsG0880046309.01.T01:intron 30.0%
GTGTCTTTAACACATGTTTA+AGG - Chr8:71818235-71818254 None:intergenic 30.0%
TCGTAAAATATAACGAGTTG+GGG + Chr8:71818694-71818713 MsG0880046309.01.T01:intron 30.0%
TGAAAAATTGAGAATGTTGG+TGG + Chr8:71818384-71818403 MsG0880046309.01.T01:intron 30.0%
TTAGCACAACATGAATTAAC+AGG - Chr8:71817514-71817533 None:intergenic 30.0%
TTATGCTGAGGTTATTCTAT+TGG + Chr8:71817155-71817174 MsG0880046309.01.T01:CDS 30.0%
TTATGTATATCGGACTAAAC+GGG - Chr8:71818642-71818661 None:intergenic 30.0%
TTATTCATACATAGTCACCT+AGG - Chr8:71818485-71818504 None:intergenic 30.0%
TTCTGCACAACTTACATTAT+TGG + Chr8:71818314-71818333 MsG0880046309.01.T01:intron 30.0%
TTCTTCAGTAATTCATAGCT+GGG + Chr8:71819161-71819180 MsG0880046309.01.T01:intron 30.0%
TTCTTGTGTTCTTAAATTGC+AGG + Chr8:71819056-71819075 MsG0880046309.01.T01:intron 30.0%
TTTCTTACTACATCCTTAGT+TGG + Chr8:71820079-71820098 MsG0880046309.01.T01:intron 30.0%
TTTCTTCAGTAATTCATAGC+TGG + Chr8:71819160-71819179 MsG0880046309.01.T01:intron 30.0%
! ATCTGACTACATTTAAAACC+CGG - Chr8:71817345-71817364 None:intergenic 30.0%
! GATTCAGGATTTTCAGTTAA+TGG + Chr8:71817819-71817838 MsG0880046309.01.T01:intron 30.0%
! TGAAGGAACAATAAGGTATT+TGG + Chr8:71816698-71816717 MsG0880046309.01.T01:five_prime_UTR 30.0%
! TGATGACATCATTTTCACTA+TGG + Chr8:71817903-71817922 MsG0880046309.01.T01:intron 30.0%
! TTCAGGATTTTCAGTTAATG+GGG + Chr8:71817821-71817840 MsG0880046309.01.T01:intron 30.0%
! TTGTATTGTTCCATAGCTTT+TGG - Chr8:71820283-71820302 None:intergenic 30.0%
! TTTTTACTATACTTCATGGC+AGG - Chr8:71818446-71818465 None:intergenic 30.0%
!! TATTGTTGATTCAAGTTGAG+TGG - Chr8:71818825-71818844 None:intergenic 30.0%
AAAAAGGGAATCATCGATGA+AGG + Chr8:71816681-71816700 MsG0880046309.01.T01:five_prime_UTR 35.0%
ACGTGCGAGTTTATGTATAT+CGG - Chr8:71818652-71818671 None:intergenic 35.0%
AGAAAAGAATACCCACAGAT+AGG + Chr8:71818988-71819007 MsG0880046309.01.T01:intron 35.0%
AGGAAATGCTAGACAACTAA+AGG + Chr8:71819680-71819699 MsG0880046309.01.T01:intron 35.0%
CAGAATTATTAGAGCACACA+AGG + Chr8:71819875-71819894 MsG0880046309.01.T01:intron 35.0%
GAGACAGACAAAATCATTCA+AGG - Chr8:71818522-71818541 None:intergenic 35.0%
GGTTGGCAACAAAAGAAAAA+GGG + Chr8:71816666-71816685 MsG0880046309.01.T01:five_prime_UTR 35.0%
GTGATTAATCAAGTATCCCA+GGG + Chr8:71818854-71818873 MsG0880046309.01.T01:intron 35.0%
GTTAAGGGCTACAAACTAAA+GGG - Chr8:71817730-71817749 None:intergenic 35.0%
TATGTATATCGGACTAAACG+GGG - Chr8:71818641-71818660 None:intergenic 35.0%
TCATCCATCAGTTGAGAAAA+GGG + Chr8:71817315-71817334 MsG0880046309.01.T01:intron 35.0%
TCATCGATGAAGGAACAATA+AGG + Chr8:71816691-71816710 MsG0880046309.01.T01:five_prime_UTR 35.0%
TGATTAATCAAGTATCCCAG+GGG + Chr8:71818855-71818874 MsG0880046309.01.T01:intron 35.0%
TGCAAATAAACAGGCATTCA+AGG + Chr8:71819123-71819142 MsG0880046309.01.T01:intron 35.0%
TGCAGAGAAAAGGAAACAAA+TGG - Chr8:71819242-71819261 None:intergenic 35.0%
TGGTTGGCAACAAAAGAAAA+AGG + Chr8:71816665-71816684 MsG0880046309.01.T01:five_prime_UTR 35.0%
TGTTAAGGGCTACAAACTAA+AGG - Chr8:71817731-71817750 None:intergenic 35.0%
TTCATCCATCAGTTGAGAAA+AGG + Chr8:71817314-71817333 MsG0880046309.01.T01:intron 35.0%
TTGTCTGTCTCCTTCTAATT+AGG + Chr8:71818531-71818550 MsG0880046309.01.T01:intron 35.0%
! AGATACATGGTCTAGAATAG+AGG - Chr8:71820353-71820372 None:intergenic 35.0%
! GATACATGGTCTAGAATAGA+GGG - Chr8:71820352-71820371 None:intergenic 35.0%
!! TTGTTTTGCAGAAACAATGC+AGG + Chr8:71817556-71817575 MsG0880046309.01.T01:intron 35.0%
!!! ATACTAGCTTGTTTTGTCCA+CGG - Chr8:71818750-71818769 None:intergenic 35.0%
AAAAAGAGGAGTGAGAGTTG+TGG + Chr8:71817072-71817091 MsG0880046309.01.T01:five_prime_UTR 40.0%
AATTCTGTCTTCACAATGCC+TGG - Chr8:71819862-71819881 None:intergenic 40.0%
ACTATACTTCATGGCAGGAA+CGG - Chr8:71818441-71818460 None:intergenic 40.0%
AGATTTGCGACTGCAATCTA+GGG - Chr8:71817474-71817493 None:intergenic 40.0%
ATGAGAGCTGTTAGGTTTGA+AGG - Chr8:71816800-71816819 None:intergenic 40.0%
ATTAGCTCAAACAGAGTCCT+AGG + Chr8:71818465-71818484 MsG0880046309.01.T01:intron 40.0%
CGTGATTAATCAAGTATCCC+AGG + Chr8:71818853-71818872 MsG0880046309.01.T01:intron 40.0%
CTCTAAGCCAAAAAACCGAA+GGG + Chr8:71820188-71820207 MsG0880046309.01.T01:CDS 40.0%
CTGTCTCCTTCTAATTAGGT+AGG + Chr8:71818535-71818554 MsG0880046309.01.T01:intron 40.0%
GAATTTGAGCCAAAACCACT+AGG - Chr8:71816737-71816756 None:intergenic 40.0%
GATTCTGCATGTGAAGGAAA+TGG + Chr8:71819375-71819394 MsG0880046309.01.T01:intron 40.0%
GGAAATGGCTAGACAACTAA+AGG + Chr8:71819390-71819409 MsG0880046309.01.T01:intron 40.0%
GGACAAAACAAGCTAGTATG+AGG + Chr8:71818751-71818770 MsG0880046309.01.T01:intron 40.0%
GTGTGGAATTCATATTCTGC+TGG - Chr8:71817708-71817727 None:intergenic 40.0%
TATACACACACATGCATGCA+CGG - Chr8:71820381-71820400 None:intergenic 40.0%
TCTAAGCCAAAAAACCGAAG+GGG + Chr8:71820189-71820208 MsG0880046309.01.T01:CDS 40.0%
TGAGAGCTGTTAGGTTTGAA+GGG - Chr8:71816799-71816818 None:intergenic 40.0%
TTCCTTCACATGCAGAATCA+TGG - Chr8:71819374-71819393 None:intergenic 40.0%
! ATCCCAGGGGTACAAAAAAA+TGG + Chr8:71818868-71818887 MsG0880046309.01.T01:intron 40.0%
! ATCTCCCTTTTCTCAACTGA+TGG - Chr8:71817322-71817341 None:intergenic 40.0%
! GCTAGAGTGTTAGCAAAAAG+AGG + Chr8:71817058-71817077 MsG0880046309.01.T01:five_prime_UTR 40.0%
! GGAACAATAAGGTATTTGGC+AGG + Chr8:71816702-71816721 MsG0880046309.01.T01:five_prime_UTR 40.0%
! TTTTCTCTTCGGAGAATTCC+AGG - Chr8:71817597-71817616 None:intergenic 40.0%
!! GTTGATTCAAGTTGAGTGGA+TGG - Chr8:71818821-71818840 None:intergenic 40.0%
!! TGCTTTAAAGACATGCTCAC+TGG + Chr8:71819201-71819220 MsG0880046309.01.T01:intron 40.0%
!! TTCAATTGAGGTGCTACTTC+TGG + Chr8:71817019-71817038 MsG0880046309.01.T01:intron 40.0%
!!! AGCCATTTTTTTGTACCCCT+GGG - Chr8:71818873-71818892 None:intergenic 40.0%
AAAAAACCGAAGGGGTGAGT+GGG + Chr8:71820197-71820216 MsG0880046309.01.T01:CDS 45.0%
AACCTAACAGCTCTCATCAC+TGG + Chr8:71816803-71816822 MsG0880046309.01.T01:five_prime_UTR 45.0%
AACGACTTAGACAAGCAGCT+TGG + Chr8:71820315-71820334 MsG0880046309.01.T01:three_prime_UTR 45.0%
ACAAAGCCATGAAGCAGTGA+CGG + Chr8:71817797-71817816 MsG0880046309.01.T01:intron 45.0%
ACTATCAATGCAGTGCATCC+AGG + Chr8:71819841-71819860 MsG0880046309.01.T01:intron 45.0%
AGCCATGATTCTGCATGTGA+AGG + Chr8:71819369-71819388 MsG0880046309.01.T01:intron 45.0%
AGTTGAGAAAAGGGAGATCC+GGG + Chr8:71817324-71817343 MsG0880046309.01.T01:intron 45.0%
ATAGGCAACAGAGAGGGATT+TGG + Chr8:71819006-71819025 MsG0880046309.01.T01:intron 45.0%
ATCCACGTTGAGGTAATTGG+GGG - Chr8:71818418-71818437 None:intergenic 45.0%
ATGCACGGTGTCTAGATACA+TGG - Chr8:71820366-71820385 None:intergenic 45.0%
CAGTTGAGAAAAGGGAGATC+CGG + Chr8:71817323-71817342 MsG0880046309.01.T01:intron 45.0%
CGAATCGAAGCCAAAAGCTA+TGG + Chr8:71820270-71820289 MsG0880046309.01.T01:CDS 45.0%
GAGATTTGCGACTGCAATCT+AGG - Chr8:71817475-71817494 None:intergenic 45.0%
GATCCACGTTGAGGTAATTG+GGG - Chr8:71818419-71818438 None:intergenic 45.0%
GCTGCTCAAAACGATATCAC+AGG + Chr8:71820028-71820047 MsG0880046309.01.T01:CDS 45.0%
GGATCCACGTTGAGGTAATT+GGG - Chr8:71818420-71818439 None:intergenic 45.0%
TACCAGTGATGAGAGCTGTT+AGG - Chr8:71816808-71816827 None:intergenic 45.0%
! AGGAGTGTACTCTCATAACC+TGG + Chr8:71817576-71817595 MsG0880046309.01.T01:CDS 45.0%
! ATGGACAGCCACTCTTTTAG+CGG + Chr8:71818595-71818614 MsG0880046309.01.T01:intron 45.0%
! GCTCTAAGCCAAAAAACCGA+AGG + Chr8:71820187-71820206 MsG0880046309.01.T01:CDS 45.0%
! GTTTGTACTGTGTTGATCCG+TGG + Chr8:71818730-71818749 MsG0880046309.01.T01:intron 45.0%
! TAGAGCACACAAGGGCATAA+CGG + Chr8:71819884-71819903 MsG0880046309.01.T01:intron 45.0%
! TCATAGCCTTTTCATGTCCC+TGG - Chr8:71817104-71817123 None:intergenic 45.0%
!! TGAGGTGCTACTTCTGGTAT+TGG + Chr8:71817025-71817044 MsG0880046309.01.T01:intron 45.0%
!!! CAGCCATTTTTTTGTACCCC+TGG - Chr8:71818874-71818893 None:intergenic 45.0%
!! ATTTATTGTATAATATAAAA+TGG + Chr8:71820513-71820532 MsG0880046309.01.T01:three_prime_UTR 5.0%
!!! AAATTTTACAAAAATATTTA+TGG - Chr8:71817859-71817878 None:intergenic 5.0%
!!! AATAAATTAATTTTTTTCTT+AGG - Chr8:71818355-71818374 None:intergenic 5.0%
AGAGGAGAGTCCTTATGCTG+AGG + Chr8:71817143-71817162 MsG0880046309.01.T01:CDS 50.0%
AGAGGAGTGAGAGTTGTGGT+TGG + Chr8:71817076-71817095 MsG0880046309.01.T01:five_prime_UTR 50.0%
AGTACCCACCGCTAAAAGAG+TGG - Chr8:71818606-71818625 None:intergenic 50.0%
CAAAAAACCGAAGGGGTGAG+TGG + Chr8:71820196-71820215 MsG0880046309.01.T01:CDS 50.0%
CAGCATAAGGACTCTCCTCT+TGG - Chr8:71817143-71817162 None:intergenic 50.0%
CCACAGATAGGCAACAGAGA+GGG + Chr8:71819000-71819019 MsG0880046309.01.T01:intron 50.0%
CCTCTCTGTTGCCTATCTGT+GGG - Chr8:71819002-71819021 None:intergenic 50.0%
CGGATCCACGTTGAGGTAAT+TGG - Chr8:71818421-71818440 None:intergenic 50.0%
CTGAATCCGTCACTGCTTCA+TGG - Chr8:71817806-71817825 None:intergenic 50.0%
GAATTTCCCACTCACCCCTT+CGG - Chr8:71820206-71820225 None:intergenic 50.0%
GAGCTGTTAGGTTTGAAGGG+AGG - Chr8:71816796-71816815 None:intergenic 50.0%
GGGCTACAAACTAAAGGGTG+TGG - Chr8:71817725-71817744 None:intergenic 50.0%
GTATATCGGACTAAACGGGG+CGG - Chr8:71818638-71818657 None:intergenic 50.0%
TATATCGGACTAAACGGGGC+GGG - Chr8:71818637-71818656 None:intergenic 50.0%
! ACAGCCACTCTTTTAGCGGT+GGG + Chr8:71818599-71818618 MsG0880046309.01.T01:intron 50.0%
! AGAGCACACAAGGGCATAAC+GGG + Chr8:71819885-71819904 MsG0880046309.01.T01:intron 50.0%
! AGGTATTTGGCAGGCATTGC+AGG + Chr8:71816711-71816730 MsG0880046309.01.T01:five_prime_UTR 50.0%
! CATTGCAGGCCTAGTGGTTT+TGG + Chr8:71816725-71816744 MsG0880046309.01.T01:five_prime_UTR 50.0%
! TCTTTTAGCGGTGGGTACTC+TGG + Chr8:71818607-71818626 MsG0880046309.01.T01:intron 50.0%
!! CATGAAGCAGTGACGGATTC+AGG + Chr8:71817804-71817823 MsG0880046309.01.T01:intron 50.0%
!! TTGGTGCCAGGGACATGAAA+AGG + Chr8:71817095-71817114 MsG0880046309.01.T01:five_prime_UTR 50.0%
!!! CACTCACCCCTTCGGTTTTT+TGG - Chr8:71820198-71820217 None:intergenic 50.0%
CCCACAGATAGGCAACAGAG+AGG + Chr8:71818999-71819018 MsG0880046309.01.T01:intron 55.0%
CCCTCTCTGTTGCCTATCTG+TGG - Chr8:71819003-71819022 None:intergenic 55.0%
TGCCCCCAATTACCTCAACG+TGG + Chr8:71818413-71818432 MsG0880046309.01.T01:intron 55.0%
TTAGCGGTGGGTACTCTGGT+TGG + Chr8:71818611-71818630 MsG0880046309.01.T01:intron 55.0%
! GACAGCCACTCTTTTAGCGG+TGG + Chr8:71818598-71818617 MsG0880046309.01.T01:intron 55.0%
! TGAGAGTTGTGGTTGGTGCC+AGG + Chr8:71817083-71817102 MsG0880046309.01.T01:five_prime_UTR 55.0%
!! GAGAGTTGTGGTTGGTGCCA+GGG + Chr8:71817084-71817103 MsG0880046309.01.T01:five_prime_UTR 55.0%
GCAGGAACGGATCCACGTTG+AGG - Chr8:71818428-71818447 None:intergenic 60.0%
! GGCAGGCATTGCAGGCCTAG+TGG + Chr8:71816719-71816738 MsG0880046309.01.T01:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 71816658 71820673 71816658 ID=MsG0880046309.01;Name=MsG0880046309.01
Chr8 mRNA 71816658 71820673 71816658 ID=MsG0880046309.01.T01;Parent=MsG0880046309.01;Name=MsG0880046309.01.T01;_AED=0.28;_eAED=0.28;_QI=258|0.75|1|1|0.75|0.6|5|367|136
Chr8 exon 71816658 71816824 71816658 ID=MsG0880046309.01.T01:exon:27901;Parent=MsG0880046309.01.T01
Chr8 exon 71817029 71817259 71817029 ID=MsG0880046309.01.T01:exon:27902;Parent=MsG0880046309.01.T01
Chr8 exon 71817567 71817646 71817567 ID=MsG0880046309.01.T01:exon:27903;Parent=MsG0880046309.01.T01
Chr8 exon 71820003 71820049 71820003 ID=MsG0880046309.01.T01:exon:27904;Parent=MsG0880046309.01.T01
Chr8 exon 71820163 71820673 71820163 ID=MsG0880046309.01.T01:exon:27905;Parent=MsG0880046309.01.T01
Chr8 five_prime_UTR 71816658 71816824 71816658 ID=MsG0880046309.01.T01:five_prime_utr;Parent=MsG0880046309.01.T01
Chr8 five_prime_UTR 71817029 71817119 71817029 ID=MsG0880046309.01.T01:five_prime_utr;Parent=MsG0880046309.01.T01
Chr8 CDS 71817120 71817259 71817120 ID=MsG0880046309.01.T01:cds;Parent=MsG0880046309.01.T01
Chr8 CDS 71817567 71817646 71817567 ID=MsG0880046309.01.T01:cds;Parent=MsG0880046309.01.T01
Chr8 CDS 71820003 71820049 71820003 ID=MsG0880046309.01.T01:cds;Parent=MsG0880046309.01.T01
Chr8 CDS 71820163 71820306 71820163 ID=MsG0880046309.01.T01:cds;Parent=MsG0880046309.01.T01
Chr8 three_prime_UTR 71820307 71820673 71820307 ID=MsG0880046309.01.T01:three_prime_utr;Parent=MsG0880046309.01.T01
Gene Sequence

>MsG0880046309.01.T01

ATGAAAGTGAAAGAAAAAATCCAAGAGGAGAGTCCTTATGCTGAGGTTATTCTATTGGAGATTGATCTTAGCTCTCTTGCTTCTGTACAAAGATTTTGCTCCGAGTTTTTAGCTTTAGATTTGCCCCTTAATATTCTCATAAACAATGCAGGAGTGTACTCTCATAACCTGGAATTCTCCGAAGAGAAAATTGAATTGACATTTGCTACAAATTACTTAGATTCCTTGTTTTTTATTGCATCAAAGCTGCTCAAAACGATATCACAGGGTGCATCAACTACTTGTTATGTTGCTCTAAGCCAAAAAACCGAAGGGGTGAGTGGGAAATTCTTTACAGATTGTAACGAGAGTAGCTGCTCAAGACTTGCAAACGACGAATCGAAGCCAAAAGCTATGGAACAATACAAATAA
Protein sequence

>MsG0880046309.01.T01

MKVKEKIQEESPYAEVILLEIDLSSLASVQRFCSEFLALDLPLNILINNAGVYSHNLEFSEEKIELTFATNYLDSLFFIASKLLKTISQGASTTCYVALSQKTEGVSGKFFTDCNESSCSRLANDESKPKAMEQYK*