Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046315.01.T01 | XP_013457784.1 | 91.329 | 173 | 11 | 2 | 1 | 171 | 9 | 179 | 9.18E-101 | 299 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046315.01.T01 | Q9Y5Z4 | 33.913 | 115 | 74 | 2 | 30 | 144 | 38 | 150 | 8.93E-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046315.01.T01 | A0A072V124 | 91.329 | 173 | 11 | 2 | 1 | 171 | 9 | 179 | 4.39e-101 | 299 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045861.01 | MsG0880046315.01 | 0.802092 | 6.608428e-49 | 3.208753e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046315.01.T01 | MTR_4g105470 | 91.329 | 173 | 11 | 2 | 1 | 171 | 9 | 179 | 1.11e-104 | 299 |
| MsG0880046315.01.T01 | MTR_8g069755 | 48.611 | 144 | 72 | 2 | 15 | 158 | 27 | 168 | 8.62e-44 | 144 |
| MsG0880046315.01.T01 | MTR_8g461390 | 39.130 | 161 | 92 | 4 | 17 | 175 | 39 | 195 | 4.95e-31 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046315.01.T01 | AT1G17100 | 37.654 | 162 | 94 | 3 | 11 | 170 | 42 | 198 | 1.04e-29 | 109 |
| MsG0880046315.01.T01 | AT1G78450 | 31.176 | 170 | 105 | 4 | 3 | 170 | 23 | 182 | 1.06e-20 | 85.5 |
| MsG0880046315.01.T01 | AT1G78460 | 35.862 | 145 | 83 | 4 | 17 | 159 | 44 | 180 | 1.02e-19 | 82.8 |
Find 58 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTTACCGACGAAGTTTC+AGG | 0.226206 | 8:+71914518 | MsG0880046315.01.T01:CDS |
| TGGAATTGAGTTTGAGTAAG+TGG | 0.360754 | 8:+71914667 | MsG0880046315.01.T01:CDS |
| GAATCCACCGCTTCCGTTAC+CGG | 0.365149 | 8:+71914542 | MsG0880046315.01.T01:CDS |
| GTAGAGTCTGATTTCGAAAT+CGG | 0.387780 | 8:-71914295 | None:intergenic |
| GATTCGAGAGGACCGGTTCC+AGG | 0.388820 | 8:-71914474 | None:intergenic |
| GTTAATACTGGTGTAGTCAT+TGG | 0.397582 | 8:-71914441 | None:intergenic |
| TAAGGATGAGAAGGTTGTTA+AGG | 0.401190 | 8:+71914632 | MsG0880046315.01.T01:CDS |
| TAACTCTTAATGTTCAATTC+CGG | 0.408026 | 8:-71914561 | None:intergenic |
| TCTGATTTCGAAATCGGATT+CGG | 0.416300 | 8:-71914289 | None:intergenic |
| AGAAATTGAGATTTGCGCCT+TGG | 0.435779 | 8:-71914410 | None:intergenic |
| GACGGTGTACTGTGGGGATT+CGG | 0.436436 | 8:-71914259 | None:intergenic |
| GATTTGCGCCTTGGATGAAC+TGG | 0.440518 | 8:-71914401 | None:intergenic |
| GAATTGAACATTAAGAGTTA+TGG | 0.441967 | 8:+71914564 | MsG0880046315.01.T01:CDS |
| GGGACGGTGGTTGTTAATAC+TGG | 0.456590 | 8:-71914453 | None:intergenic |
| ACAACCACCGTCCCTGGAAC+CGG | 0.457006 | 8:+71914462 | MsG0880046315.01.T01:CDS |
| GTATTCATTGCGCAGTATAA+TGG | 0.460946 | 8:+71914721 | MsG0880046315.01.T01:CDS |
| TGAGAAGGTTGTTAAGGAAG+CGG | 0.466853 | 8:+71914638 | MsG0880046315.01.T01:CDS |
| GTATTAACAACCACCGTCCC+TGG | 0.471177 | 8:+71914456 | MsG0880046315.01.T01:CDS |
| TTCGAAGGAGATGATGTTTG+TGG | 0.475977 | 8:-71914346 | None:intergenic |
| TTCAATTCCGGTAACGGAAG+CGG | 0.476760 | 8:-71914549 | None:intergenic |
| TGTTAAGGAAGCGGAGAAGC+TGG | 0.477382 | 8:+71914647 | MsG0880046315.01.T01:CDS |
| GACTCTACCGAACCTCCGTC+TGG | 0.479846 | 8:+71914310 | MsG0880046315.01.T01:CDS |
| CGCAGCGATGTGTTTGATCA+TGG | 0.486262 | 8:+71914206 | None:intergenic |
| GCCTTGTGATTCGAGAGGAC+CGG | 0.498007 | 8:-71914481 | None:intergenic |
| AGTGATGCGAAGAGGAAAGG+TGG | 0.501346 | 8:+71914698 | MsG0880046315.01.T01:CDS |
| TGAGTTTGAGTAAGTGGGGT+GGG | 0.502026 | 8:+71914673 | MsG0880046315.01.T01:CDS |
| TTAATGTTCAATTCCGGTAA+CGG | 0.507064 | 8:-71914555 | None:intergenic |
| GTGATGCGAAGAGGAAAGGT+GGG | 0.515588 | 8:+71914699 | MsG0880046315.01.T01:CDS |
| GTCATTGTGTTGCGGTGAGA+GGG | 0.517068 | 8:+71914595 | MsG0880046315.01.T01:CDS |
| GAGAGTGATGCGAAGAGGAA+AGG | 0.518356 | 8:+71914695 | MsG0880046315.01.T01:CDS |
| TGTGGACGACGGTGTACTGT+GGG | 0.522652 | 8:-71914266 | None:intergenic |
| GGAATTGAGTTTGAGTAAGT+GGG | 0.535796 | 8:+71914668 | MsG0880046315.01.T01:CDS |
| TGGGTTTGCTAAGGATGAGA+AGG | 0.541542 | 8:+71914623 | MsG0880046315.01.T01:CDS |
| CGGACATCCAGACGGAGGTT+CGG | 0.541589 | 8:-71914317 | None:intergenic |
| AAGGAGATGATGTTTGTGGC+AGG | 0.570633 | 8:-71914342 | None:intergenic |
| GTGTGGACGACGGTGTACTG+TGG | 0.577621 | 8:-71914267 | None:intergenic |
| GATGTGTTTGATCATGGCTA+TGG | 0.581034 | 8:+71914212 | None:intergenic |
| AGTCATTGTGTTGCGGTGAG+AGG | 0.583912 | 8:+71914594 | MsG0880046315.01.T01:CDS |
| ATTCGAGAGGACCGGTTCCA+GGG | 0.589647 | 8:-71914473 | None:intergenic |
| GAGAGGACCGGTTCCAGGGA+CGG | 0.594735 | 8:-71914469 | None:intergenic |
| AGACTCTTCCAGTTCATCCA+AGG | 0.596957 | 8:+71914393 | MsG0880046315.01.T01:CDS |
| GGATGAACTGGAAGAGTCTG+TGG | 0.597193 | 8:-71914389 | None:intergenic |
| AATTCCGGTAACGGAAGCGG+TGG | 0.603443 | 8:-71914546 | None:intergenic |
| TCTGTGGAAACCGTTTCGTG+TGG | 0.614617 | 8:-71914373 | None:intergenic |
| ATCGGATTCGGTGTGGACGA+CGG | 0.615803 | 8:-71914277 | None:intergenic |
| TTGAGTTTGAGTAAGTGGGG+TGG | 0.620072 | 8:+71914672 | MsG0880046315.01.T01:CDS |
| GGTGTACTGTGGGGATTCGG+TGG | 0.624769 | 8:-71914256 | None:intergenic |
| GGCAGGTGCGGACATCCAGA+CGG | 0.624901 | 8:-71914325 | None:intergenic |
| GGTGGGAGAGTGATGCGAAG+AGG | 0.630978 | 8:+71914690 | MsG0880046315.01.T01:CDS |
| TTTCGAAATCGGATTCGGTG+TGG | 0.638537 | 8:-71914284 | None:intergenic |
| GGATTCTCCTGAAACTTCGT+CGG | 0.639976 | 8:-71914525 | None:intergenic |
| GAATTGAGTTTGAGTAAGTG+GGG | 0.647982 | 8:+71914669 | MsG0880046315.01.T01:CDS |
| GATGATGTTTGTGGCAGGTG+CGG | 0.662016 | 8:-71914337 | None:intergenic |
| ACCGGTCCTCTCGAATCACA+AGG | 0.680815 | 8:+71914480 | MsG0880046315.01.T01:CDS |
| AGGACCGGTTCCAGGGACGG+TGG | 0.683844 | 8:-71914466 | None:intergenic |
| AGGTGCGGACATCCAGACGG+AGG | 0.685159 | 8:-71914322 | None:intergenic |
| TAGTAGCCTTGTGATTCGAG+AGG | 0.692619 | 8:-71914486 | None:intergenic |
| GTGGACGACGGTGTACTGTG+GGG | 0.759851 | 8:-71914265 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAATTGAACATTAAGAGTTA+TGG | + | Chr8:71914564-71914583 | MsG0880046315.01.T01:CDS | 25.0% |
| ! | TAACTCTTAATGTTCAATTC+CGG | - | Chr8:71914564-71914583 | None:intergenic | 25.0% |
| TTAATGTTCAATTCCGGTAA+CGG | - | Chr8:71914558-71914577 | None:intergenic | 30.0% | |
| GAATTGAGTTTGAGTAAGTG+GGG | + | Chr8:71914669-71914688 | MsG0880046315.01.T01:CDS | 35.0% | |
| GGAATTGAGTTTGAGTAAGT+GGG | + | Chr8:71914668-71914687 | MsG0880046315.01.T01:CDS | 35.0% | |
| GTAGAGTCTGATTTCGAAAT+CGG | - | Chr8:71914298-71914317 | None:intergenic | 35.0% | |
| GTTAATACTGGTGTAGTCAT+TGG | - | Chr8:71914444-71914463 | None:intergenic | 35.0% | |
| TAAGGATGAGAAGGTTGTTA+AGG | + | Chr8:71914632-71914651 | MsG0880046315.01.T01:CDS | 35.0% | |
| TCTGATTTCGAAATCGGATT+CGG | - | Chr8:71914292-71914311 | None:intergenic | 35.0% | |
| TGGAATTGAGTTTGAGTAAG+TGG | + | Chr8:71914667-71914686 | MsG0880046315.01.T01:CDS | 35.0% | |
| !! | TTTTGAGAGTCATTGTGTTG+CGG | + | Chr8:71914587-71914606 | MsG0880046315.01.T01:CDS | 35.0% |
| AGAAATTGAGATTTGCGCCT+TGG | - | Chr8:71914413-71914432 | None:intergenic | 40.0% | |
| CTATTTACCGACGAAGTTTC+AGG | + | Chr8:71914518-71914537 | MsG0880046315.01.T01:CDS | 40.0% | |
| TGAGAAGGTTGTTAAGGAAG+CGG | + | Chr8:71914638-71914657 | MsG0880046315.01.T01:CDS | 40.0% | |
| TTCGAAAAAGCCACACGAAA+CGG | + | Chr8:71914363-71914382 | MsG0880046315.01.T01:CDS | 40.0% | |
| TTCGAAGGAGATGATGTTTG+TGG | - | Chr8:71914349-71914368 | None:intergenic | 40.0% | |
| AAGGAGATGATGTTTGTGGC+AGG | - | Chr8:71914345-71914364 | None:intergenic | 45.0% | |
| AGACTCTTCCAGTTCATCCA+AGG | + | Chr8:71914393-71914412 | MsG0880046315.01.T01:CDS | 45.0% | |
| GGATTCTCCTGAAACTTCGT+CGG | - | Chr8:71914528-71914547 | None:intergenic | 45.0% | |
| TGAGTTTGAGTAAGTGGGGT+GGG | + | Chr8:71914673-71914692 | MsG0880046315.01.T01:CDS | 45.0% | |
| TTGAGTTTGAGTAAGTGGGG+TGG | + | Chr8:71914672-71914691 | MsG0880046315.01.T01:CDS | 45.0% | |
| !! | TAGTAGCCTTGTGATTCGAG+AGG | - | Chr8:71914489-71914508 | None:intergenic | 45.0% |
| !! | TGGGTTTGCTAAGGATGAGA+AGG | + | Chr8:71914623-71914642 | MsG0880046315.01.T01:CDS | 45.0% |
| !! | TTCAATTCCGGTAACGGAAG+CGG | - | Chr8:71914552-71914571 | None:intergenic | 45.0% |
| !! | TTTCGAAATCGGATTCGGTG+TGG | - | Chr8:71914287-71914306 | None:intergenic | 45.0% |
| !!! | AGGGTTTTCTGGGTTTGCTA+AGG | + | Chr8:71914614-71914633 | MsG0880046315.01.T01:CDS | 45.0% |
| !!! | GTTTCGTGTGGCTTTTTCGA+AGG | - | Chr8:71914364-71914383 | None:intergenic | 45.0% |
| AGTCATTGTGTTGCGGTGAG+AGG | + | Chr8:71914594-71914613 | MsG0880046315.01.T01:CDS | 50.0% | |
| AGTGATGCGAAGAGGAAAGG+TGG | + | Chr8:71914698-71914717 | MsG0880046315.01.T01:CDS | 50.0% | |
| GAGAGTGATGCGAAGAGGAA+AGG | + | Chr8:71914695-71914714 | MsG0880046315.01.T01:CDS | 50.0% | |
| GATGATGTTTGTGGCAGGTG+CGG | - | Chr8:71914340-71914359 | None:intergenic | 50.0% | |
| GATTTGCGCCTTGGATGAAC+TGG | - | Chr8:71914404-71914423 | None:intergenic | 50.0% | |
| GGATGAACTGGAAGAGTCTG+TGG | - | Chr8:71914392-71914411 | None:intergenic | 50.0% | |
| GGGACGGTGGTTGTTAATAC+TGG | - | Chr8:71914456-71914475 | None:intergenic | 50.0% | |
| GTATTAACAACCACCGTCCC+TGG | + | Chr8:71914456-71914475 | MsG0880046315.01.T01:CDS | 50.0% | |
| GTCATTGTGTTGCGGTGAGA+GGG | + | Chr8:71914595-71914614 | MsG0880046315.01.T01:CDS | 50.0% | |
| GTGATGCGAAGAGGAAAGGT+GGG | + | Chr8:71914699-71914718 | MsG0880046315.01.T01:CDS | 50.0% | |
| TCTGTGGAAACCGTTTCGTG+TGG | - | Chr8:71914376-71914395 | None:intergenic | 50.0% | |
| TGTTAAGGAAGCGGAGAAGC+TGG | + | Chr8:71914647-71914666 | MsG0880046315.01.T01:CDS | 50.0% | |
| ! | TTGCGGTGAGAGGGTTTTCT+GGG | + | Chr8:71914604-71914623 | MsG0880046315.01.T01:CDS | 50.0% |
| ACCGGTCCTCTCGAATCACA+AGG | + | Chr8:71914480-71914499 | MsG0880046315.01.T01:CDS | 55.0% | |
| GAATCCACCGCTTCCGTTAC+CGG | + | Chr8:71914542-71914561 | MsG0880046315.01.T01:CDS | 55.0% | |
| GACGGTGTACTGTGGGGATT+CGG | - | Chr8:71914262-71914281 | None:intergenic | 55.0% | |
| GCCTTGTGATTCGAGAGGAC+CGG | - | Chr8:71914484-71914503 | None:intergenic | 55.0% | |
| TGTGGACGACGGTGTACTGT+GGG | - | Chr8:71914269-71914288 | None:intergenic | 55.0% | |
| ! | ATTCGAGAGGACCGGTTCCA+GGG | - | Chr8:71914476-71914495 | None:intergenic | 55.0% |
| ! | GTTGCGGTGAGAGGGTTTTC+TGG | + | Chr8:71914603-71914622 | MsG0880046315.01.T01:CDS | 55.0% |
| !! | AATTCCGGTAACGGAAGCGG+TGG | - | Chr8:71914549-71914568 | None:intergenic | 55.0% |
| !! | ATCGGATTCGGTGTGGACGA+CGG | - | Chr8:71914280-71914299 | None:intergenic | 55.0% |
| ACAACCACCGTCCCTGGAAC+CGG | + | Chr8:71914462-71914481 | MsG0880046315.01.T01:CDS | 60.0% | |
| CGGACATCCAGACGGAGGTT+CGG | - | Chr8:71914320-71914339 | None:intergenic | 60.0% | |
| GACTCTACCGAACCTCCGTC+TGG | + | Chr8:71914310-71914329 | MsG0880046315.01.T01:CDS | 60.0% | |
| GGTGGGAGAGTGATGCGAAG+AGG | + | Chr8:71914690-71914709 | MsG0880046315.01.T01:CDS | 60.0% | |
| GGTGTACTGTGGGGATTCGG+TGG | - | Chr8:71914259-71914278 | None:intergenic | 60.0% | |
| GTGGACGACGGTGTACTGTG+GGG | - | Chr8:71914268-71914287 | None:intergenic | 60.0% | |
| GTGTGGACGACGGTGTACTG+TGG | - | Chr8:71914270-71914289 | None:intergenic | 60.0% | |
| ! | GATTCGAGAGGACCGGTTCC+AGG | - | Chr8:71914477-71914496 | None:intergenic | 60.0% |
| AGGTGCGGACATCCAGACGG+AGG | - | Chr8:71914325-71914344 | None:intergenic | 65.0% | |
| GGCAGGTGCGGACATCCAGA+CGG | - | Chr8:71914328-71914347 | None:intergenic | 65.0% | |
| ! | GAGAGGACCGGTTCCAGGGA+CGG | - | Chr8:71914472-71914491 | None:intergenic | 65.0% |
| ! | AGGACCGGTTCCAGGGACGG+TGG | - | Chr8:71914469-71914488 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 71914213 | 71914740 | 71914213 | ID=MsG0880046315.01;Name=MsG0880046315.01 |
| Chr8 | mRNA | 71914213 | 71914740 | 71914213 | ID=MsG0880046315.01.T01;Parent=MsG0880046315.01;Name=MsG0880046315.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|175 |
| Chr8 | exon | 71914213 | 71914740 | 71914213 | ID=MsG0880046315.01.T01:exon:27944;Parent=MsG0880046315.01.T01 |
| Chr8 | CDS | 71914213 | 71914740 | 71914213 | ID=MsG0880046315.01.T01:cds;Parent=MsG0880046315.01.T01 |
| Gene Sequence |
| Protein sequence |
