AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047622.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047622.01.T01 MTR_4g131670 75.556 270 13 1 18 234 44 313 7.01e-130 370
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047622.01.T01 AT5G58200 46.008 263 75 7 18 223 39 291 7.65e-61 194
MsG0880047622.01.T01 AT5G58200 58.101 179 63 5 47 223 36 204 1.93e-60 191

Find 69 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTACCAATAGCAGCTTTC+TGG 0.136726 8:+88862881 None:intergenic
TTCACGCGCCTACCTTATTT+TGG 0.210571 8:-88866142 MsG0880047622.01.T01:five_prime_UTR
TTTGCTAAAGCAGTTATATT+AGG 0.232537 8:-88864654 MsG0880047622.01.T01:CDS
CTTCGTGCAGGCCTTGGTTC+TGG 0.268641 8:-88862742 MsG0880047622.01.T01:intron
ACGATATATTCATTTATATA+AGG 0.297563 8:+88861880 None:intergenic
CTGATATTACTTGCACATAA+TGG 0.297738 8:-88862844 MsG0880047622.01.T01:CDS
CGAGGACATGAGAAGCTTAA+AGG 0.316354 8:-88862164 MsG0880047622.01.T01:CDS
TTTGGTTAAATCGTTGTTGT+AGG 0.355829 8:-88866124 MsG0880047622.01.T01:five_prime_UTR
ATGATATATGTGGAAAAGAT+TGG 0.358927 8:-88862714 MsG0880047622.01.T01:CDS
ACAACGATTTAACCAAAATA+AGG 0.370630 8:+88866130 None:intergenic
TATTAGGAAACCATGATTCC+TGG 0.379064 8:-88864638 MsG0880047622.01.T01:CDS
ACAACATCATATACTTGAAT+GGG 0.396824 8:-88862219 MsG0880047622.01.T01:CDS
TAATCAGGTATCTATTCCTT+TGG 0.398444 8:-88862314 MsG0880047622.01.T01:CDS
TCTATTCCTTTGGTTGTGTT+CGG 0.404828 8:-88862304 MsG0880047622.01.T01:CDS
TGGAGGGCTCCACTCTTTGT+TGG 0.416185 8:-88866179 None:intergenic
TGAATGGGGCCATTGTTCCT+AGG 0.416349 8:-88862204 MsG0880047622.01.T01:CDS
TTTGCCTCTAGTGAGGAAAT+AGG 0.423873 8:+88862133 None:intergenic
GACAACATCATATACTTGAA+TGG 0.432447 8:-88862220 MsG0880047622.01.T01:CDS
TAAATCGTTGTTGTAGGAGT+TGG 0.440245 8:-88866118 MsG0880047622.01.T01:five_prime_UTR
AGAATCCAGAAAGCTGCTAT+TGG 0.442023 8:-88862886 MsG0880047622.01.T01:CDS
TTAGGAAAATGATTGTCGTT+GGG 0.446203 8:-88862246 MsG0880047622.01.T01:CDS
AGCAGCTTTCTGGATTCTCT+TGG 0.452550 8:+88862891 None:intergenic
TGATATATGTGGAAAAGATT+GGG 0.453237 8:-88862713 MsG0880047622.01.T01:CDS
TCTGTTCGAATTGTGGTTGT+CGG 0.454517 8:-88866034 MsG0880047622.01.T01:five_prime_UTR
TACCCAACTTTCTGCAACTC+TGG 0.455845 8:+88862060 None:intergenic
TCTTTCTTCTGTTCGAATTG+TGG 0.456514 8:-88866041 MsG0880047622.01.T01:five_prime_UTR
TACCAGAGTTGCAGAAAGTT+GGG 0.460117 8:-88862062 MsG0880047622.01.T01:CDS
TTACCAGAGTTGCAGAAAGT+TGG 0.462443 8:-88862063 MsG0880047622.01.T01:CDS
GCTTAGTAAACCAGGAATCA+TGG 0.463502 8:+88864628 None:intergenic
TTTAGGAAAATGATTGTCGT+TGG 0.470518 8:-88862247 MsG0880047622.01.T01:CDS
CTGGAGGAATTGAAGTGCCT+TGG 0.477477 8:+88865868 None:intergenic
CTTGCTAAAAGAGAATAATC+AGG 0.482095 8:-88862329 MsG0880047622.01.T01:CDS
ATGTGGAAAAGATTGGGAAT+TGG 0.484047 8:-88862707 MsG0880047622.01.T01:CDS
AATATCAGAAAATGATCCTC+TGG 0.484452 8:+88862859 None:intergenic
CTTTACTTTAGTTGAGCTCT+CGG 0.486107 8:-88862095 MsG0880047622.01.T01:CDS
TTGGATGGTGATGGTGATCA+TGG 0.502841 8:-88862688 MsG0880047622.01.T01:CDS
CAAAGAGCTCGCACATGGCA+AGG 0.507452 8:-88862272 MsG0880047622.01.T01:CDS
CGAGAGCTCAACTAAAGTAA+AGG 0.527148 8:+88862096 None:intergenic
GAATGGGGCCATTGTTCCTA+GGG 0.535631 8:-88862203 MsG0880047622.01.T01:CDS
ATTTCCTCACTAGAGGCAAA+TGG 0.535934 8:-88862130 MsG0880047622.01.T01:CDS
GCTTTGTTGCTCGACTTCAA+TGG 0.536809 8:-88866087 MsG0880047622.01.T01:five_prime_UTR
GAACTTTCAACAAGATTCCA+AGG 0.547092 8:-88865885 MsG0880047622.01.T01:CDS
TGCATAGATGAAACAGTTCG+AGG 0.557370 8:-88861924 MsG0880047622.01.T01:exon
TAGGAAAATGATTGTCGTTG+GGG 0.566303 8:-88862245 MsG0880047622.01.T01:CDS
AAAAGAACAATATTATTCGA+AGG 0.567058 8:-88862011 MsG0880047622.01.T01:CDS
GACGTGCTGCCAACAAAGAG+TGG 0.579605 8:+88866170 None:intergenic
ACTTTAGTTGAGCTCTCGGA+AGG 0.587799 8:-88862091 MsG0880047622.01.T01:CDS
AAGTCCTATTTCCTCACTAG+AGG 0.594070 8:-88862137 MsG0880047622.01.T01:CDS
GTGCGAGCTCTTTGTGCATG+TGG 0.604383 8:+88862281 None:intergenic
AAACGACAACTTAGAAGATG+AGG 0.606215 8:+88861971 None:intergenic
GGGTATCAGTTATTGACGAT+AGG 0.606861 8:-88862042 MsG0880047622.01.T01:CDS
TGTACCATTTGCCTCTAGTG+AGG 0.611667 8:+88862126 None:intergenic
AATTGTGGTTGTCGGTGATG+TGG 0.620767 8:-88866026 MsG0880047622.01.T01:intron
AGAAAACCGCTTAGTAAACC+AGG 0.621108 8:+88864620 None:intergenic
AAAGAGCTCGCACATGGCAA+GGG 0.623466 8:-88862271 MsG0880047622.01.T01:CDS
CGATTTAACCAAAATAAGGT+AGG 0.626542 8:+88866134 None:intergenic
CTTGCACATAATGGACCCAC+AGG 0.631104 8:-88862835 MsG0880047622.01.T01:intron
GATTGGGAATTGGATGGTGA+TGG 0.631826 8:-88862697 MsG0880047622.01.T01:CDS
TCTGGTTCGAATGATATATG+TGG 0.634276 8:-88862724 MsG0880047622.01.T01:CDS
ATGATTCCTGGTTTACTAAG+CGG 0.635427 8:-88864626 MsG0880047622.01.T01:CDS
GATGGTGATCATGGTGACCC+AGG 0.642301 8:-88862679 MsG0880047622.01.T01:intron
ATGTGGCCGAACACAACCAA+AGG 0.646151 8:+88862298 None:intergenic
ATGCACAAAGAGCTCGCACA+TGG 0.647134 8:-88862277 MsG0880047622.01.T01:CDS
GGTCAAAAGTTTGAGCGACG+AGG 0.662003 8:-88862182 MsG0880047622.01.T01:CDS
AGCTGCTATTGGTACACCAG+AGG 0.664764 8:-88862875 MsG0880047622.01.T01:CDS
TAGATATGGAGTTAAAGACA+TGG 0.667486 8:-88862923 MsG0880047622.01.T01:CDS
CAACATCATATACTTGAATG+GGG 0.669817 8:-88862218 MsG0880047622.01.T01:CDS
GGAAAAGATTGGGAATTGGA+TGG 0.680179 8:-88862703 MsG0880047622.01.T01:CDS
TATCATTCGAACCAGAACCA+AGG 0.710976 8:+88862731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAATATTTAACATTAATT+TGG + Chr8:88865491-88865510 None:intergenic 10.0%
!! GTTCATATAAATAAAAATAA+AGG + Chr8:88862791-88862810 None:intergenic 10.0%
!! TATAAAATTGATTACTTTAT+CGG + Chr8:88864820-88864839 None:intergenic 10.0%
!!! TATACTTTTTTAATCTTAAA+TGG - Chr8:88864852-88864871 MsG0880047622.01.T01:intron 10.0%
!! ACGATATATTCATTTATATA+AGG + Chr8:88866161-88866180 None:intergenic 15.0%
!! ATAAAATTGATTACTTTATC+GGG + Chr8:88864819-88864838 None:intergenic 15.0%
!! ATACTTTCATTAAAAGAAAA+TGG - Chr8:88864019-88864038 MsG0880047622.01.T01:intron 15.0%
!! ATATCAATTATGATTTACAA+TGG + Chr8:88865075-88865094 None:intergenic 15.0%
!! ATATCTATCAATTAATCATT+AGG - Chr8:88863100-88863119 MsG0880047622.01.T01:intron 15.0%
!! GTATATATTAATTATTACCT+GGG + Chr8:88865379-88865398 None:intergenic 15.0%
!! TACTTTCATTAAAAGAAAAT+GGG - Chr8:88864020-88864039 MsG0880047622.01.T01:intron 15.0%
!! TATAATGAGAATAATTATAC+TGG + Chr8:88862192-88862211 None:intergenic 15.0%
!! TGTATATATTAATTATTACC+TGG + Chr8:88865380-88865399 None:intergenic 15.0%
!!! AAAATAAAATCTTGTGTTTT+TGG - Chr8:88865421-88865440 MsG0880047622.01.T01:intron 15.0%
!!! AAACTTAAATCCATTTTTTT+TGG + Chr8:88863684-88863703 None:intergenic 15.0%
!!! ATAATATTGTTCTTTTAATG+TGG + Chr8:88866023-88866042 None:intergenic 15.0%
!!! TTCATTTTAAAACAAATGAT+GGG - Chr8:88863537-88863556 MsG0880047622.01.T01:intron 15.0%
!!! TTTCATTTTAAAACAAATGA+TGG - Chr8:88863536-88863555 MsG0880047622.01.T01:intron 15.0%
!! AAAAGAACAATATTATTCGA+AGG - Chr8:88866027-88866046 MsG0880047622.01.T01:exon 20.0%
!! CTAATTGAGAATGTAAATTA+CGG - Chr8:88862281-88862300 MsG0880047622.01.T01:CDS 20.0%
!! TAAGGTACAAAATTAATTCT+TGG + Chr8:88863861-88863880 None:intergenic 20.0%
!! TGAAATACGTAATTAACATA+TGG + Chr8:88863710-88863729 None:intergenic 20.0%
!! TGATTTAACCTAGTTTATTA+TGG - Chr8:88865656-88865675 MsG0880047622.01.T01:intron 20.0%
!! TTAAATTCATTTAGTGTTGT+TGG - Chr8:88862942-88862961 MsG0880047622.01.T01:intron 20.0%
!!! AGAAAATTCAGGAATTATTT+TGG + Chr8:88865278-88865297 None:intergenic 20.0%
!!! ATTTAACATTAATTTGGAGT+GGG + Chr8:88865485-88865504 None:intergenic 20.0%
!!! CTTTTTTAATCTTAAATGGA+TGG - Chr8:88864856-88864875 MsG0880047622.01.T01:intron 20.0%
!!! TAATATCCATTGTTTTCATA+AGG - Chr8:88864957-88864976 MsG0880047622.01.T01:intron 20.0%
!!! TATTCTGTGTAATTTTCTAA+TGG - Chr8:88862468-88862487 MsG0880047622.01.T01:intron 20.0%
!!! TATTTAACATTAATTTGGAG+TGG + Chr8:88865486-88865505 None:intergenic 20.0%
!!! TCTCTTGAGTTAATATATTT+TGG - Chr8:88862427-88862446 MsG0880047622.01.T01:intron 20.0%
!!! TTAATTTTGTTAGGTGATTT+TGG - Chr8:88863318-88863337 MsG0880047622.01.T01:intron 20.0%
!!! TTTGAACTTTTTCTTAGATA+TGG - Chr8:88865101-88865120 MsG0880047622.01.T01:intron 20.0%
!!! TTTGTTTTCTTCATTTCAAA+TGG - Chr8:88865506-88865525 MsG0880047622.01.T01:intron 20.0%
!!! TTTGTTTTTATAGAATCAGT+TGG - Chr8:88864228-88864247 MsG0880047622.01.T01:intron 20.0%
! AAATATCAACATAGTGTGAT+AGG + Chr8:88862921-88862940 None:intergenic 25.0%
! AACAATAAAATTTCCATGCT+AGG + Chr8:88863905-88863924 None:intergenic 25.0%
! AATGAGAATAATTATACTGG+AGG + Chr8:88862189-88862208 None:intergenic 25.0%
! AATTAAGCGACAAAATTTGT+AGG + Chr8:88862602-88862621 None:intergenic 25.0%
! AATTAATCATTAGGTGAGAA+TGG - Chr8:88863109-88863128 MsG0880047622.01.T01:intron 25.0%
! ACAACATCATATACTTGAAT+GGG - Chr8:88865819-88865838 MsG0880047622.01.T01:intron 25.0%
! ACAACGATTTAACCAAAATA+AGG + Chr8:88861911-88861930 None:intergenic 25.0%
! ACACAAAGAAAAATGATTTG+AGG + Chr8:88863468-88863487 None:intergenic 25.0%
! ACATCAGAAACATAAATAGA+TGG + Chr8:88864892-88864911 None:intergenic 25.0%
! AGATTACTTAAAAGCCTAAT+TGG - Chr8:88864167-88864186 MsG0880047622.01.T01:intron 25.0%
! ATGATATATGTGGAAAAGAT+TGG - Chr8:88865324-88865343 MsG0880047622.01.T01:intron 25.0%
! ATTAATCAGTGTAGTTGAAA+AGG + Chr8:88863303-88863322 None:intergenic 25.0%
! ATTGTAAACCAACAACAAAT+AGG - Chr8:88864774-88864793 MsG0880047622.01.T01:intron 25.0%
! GAATTCAACACAAATTTCAA+AGG + Chr8:88863013-88863032 None:intergenic 25.0%
! GAGAATGGTATATGTATTAT+TGG - Chr8:88863124-88863143 MsG0880047622.01.T01:intron 25.0%
! GATATGCGTATATAATATGT+GGG - Chr8:88863981-88864000 MsG0880047622.01.T01:intron 25.0%
! TGATATATGTGGAAAAGATT+GGG - Chr8:88865325-88865344 MsG0880047622.01.T01:intron 25.0%
! TGATATGCGTATATAATATG+TGG - Chr8:88863980-88863999 MsG0880047622.01.T01:intron 25.0%
! TTAATATATACATGAGAGCT+TGG - Chr8:88865388-88865407 MsG0880047622.01.T01:intron 25.0%
! TTATGCAACCATAATAAACT+AGG + Chr8:88865667-88865686 None:intergenic 25.0%
! TTTGCTAAAGCAGTTATATT+AGG - Chr8:88863384-88863403 MsG0880047622.01.T01:intron 25.0%
!! AACACAATCGTTTTCTTTAT+AGG + Chr8:88862709-88862728 None:intergenic 25.0%
!! ACTTTTCAGTAAATCTAGAA+AGG + Chr8:88864195-88864214 None:intergenic 25.0%
!! TGAAATTATCTAAAGTCTTG+TGG + Chr8:88863521-88863540 None:intergenic 25.0%
!! TTTAACATTAATTTGGAGTG+GGG + Chr8:88865484-88865503 None:intergenic 25.0%
!!! AATCTTGTGTTTTTGGTTAT+TGG - Chr8:88865428-88865447 MsG0880047622.01.T01:intron 25.0%
!!! CTACACTGATTAATTTTGTT+AGG - Chr8:88863309-88863328 MsG0880047622.01.T01:intron 25.0%
!!! TCAGATATATGCTTGTTATT+AGG + Chr8:88862813-88862832 None:intergenic 25.0%
!!! TTAAAACATTGAGTTGACAT+TGG + Chr8:88864473-88864492 None:intergenic 25.0%
AAAAAGATAAGAAGCTGTCT+CGG - Chr8:88862518-88862537 MsG0880047622.01.T01:intron 30.0%
AAAATTTGTAGGTTTCCTGA+TGG + Chr8:88862591-88862610 None:intergenic 30.0%
AAGGTGTGTATTACTTGTAA+AGG - Chr8:88864416-88864435 MsG0880047622.01.T01:intron 30.0%
AATACCATACATTATCTTCC+TGG + Chr8:88865034-88865053 None:intergenic 30.0%
AATATCAGAAAATGATCCTC+TGG + Chr8:88865182-88865201 None:intergenic 30.0%
AATTCTTTCAAACCCTCAAT+GGG + Chr8:88864066-88864085 None:intergenic 30.0%
ATACCATACATTATCTTCCT+GGG + Chr8:88865033-88865052 None:intergenic 30.0%
ATGTATTATTGGTGTGATAG+CGG - Chr8:88863135-88863154 MsG0880047622.01.T01:intron 30.0%
CAACATCATATACTTGAATG+GGG - Chr8:88865820-88865839 MsG0880047622.01.T01:intron 30.0%
CAGTCATGCTTAAAAAGATT+AGG + Chr8:88862130-88862149 None:intergenic 30.0%
CATGCTTAAAAAGATTAGGA+AGG + Chr8:88862126-88862145 None:intergenic 30.0%
CGATTTAACCAAAATAAGGT+AGG + Chr8:88861907-88861926 None:intergenic 30.0%
CTGATATTACTTGCACATAA+TGG - Chr8:88865194-88865213 MsG0880047622.01.T01:intron 30.0%
CTTATGCCTTATGAAAACAA+TGG + Chr8:88864966-88864985 None:intergenic 30.0%
CTTGCTAAAAGAGAATAATC+AGG - Chr8:88865709-88865728 MsG0880047622.01.T01:intron 30.0%
CTTGTTGGGATTAAAAACAA+AGG + Chr8:88864504-88864523 None:intergenic 30.0%
GAAATTTGTGTTGAATTCCA+TGG - Chr8:88863015-88863034 MsG0880047622.01.T01:intron 30.0%
GACAACATCATATACTTGAA+TGG - Chr8:88865818-88865837 MsG0880047622.01.T01:intron 30.0%
GTTTGCTTCAAAATAGTTTG+AGG - Chr8:88864600-88864619 MsG0880047622.01.T01:intron 30.0%
TAATCAGGTATCTATTCCTT+TGG - Chr8:88865724-88865743 MsG0880047622.01.T01:intron 30.0%
TACGGAAGCAATCAAATTAA+GGG - Chr8:88862299-88862318 MsG0880047622.01.T01:CDS 30.0%
TAGATATGGAGTTAAAGACA+TGG - Chr8:88865115-88865134 MsG0880047622.01.T01:intron 30.0%
TATTACAGTCAAGTAACTCT+AGG + Chr8:88864942-88864961 None:intergenic 30.0%
TGATTTAATTGTCAGTGAGA+AGG - Chr8:88863803-88863822 MsG0880047622.01.T01:intron 30.0%
TTACGGAAGCAATCAAATTA+AGG - Chr8:88862298-88862317 MsG0880047622.01.T01:CDS 30.0%
TTAGGAAAATGATTGTCGTT+GGG - Chr8:88865792-88865811 MsG0880047622.01.T01:intron 30.0%
TTGTGTGTGTTAAATTTACG+TGG - Chr8:88863482-88863501 MsG0880047622.01.T01:intron 30.0%
TTTAGGAAAATGATTGTCGT+TGG - Chr8:88865791-88865810 MsG0880047622.01.T01:intron 30.0%
TTTGGTTAAATCGTTGTTGT+AGG - Chr8:88861914-88861933 MsG0880047622.01.T01:three_prime_UTR 30.0%
! ATGATGGGATACAATAACAA+TGG - Chr8:88863552-88863571 MsG0880047622.01.T01:intron 30.0%
! CTAATAAAAGTAAGTGTCGT+TGG - Chr8:88864336-88864355 MsG0880047622.01.T01:intron 30.0%
! CTGATTATTCTCTTTTAGCA+AGG + Chr8:88865711-88865730 None:intergenic 30.0%
! TCTTATTTGCTCCAATTATG+TGG - Chr8:88864536-88864555 MsG0880047622.01.T01:intron 30.0%
! TTAACATTAATTTGGAGTGG+GGG + Chr8:88865483-88865502 None:intergenic 30.0%
!! AAAATTAAGCGACTGACTTT+TGG + Chr8:88862651-88862670 None:intergenic 30.0%
!! AAGTACAACCTATTTGTTGT+TGG + Chr8:88864785-88864804 None:intergenic 30.0%
!! ACGACACTTACTTTTATTAG+AGG + Chr8:88864336-88864355 None:intergenic 30.0%
!! TGAGGAAAATACCACTTTTA+AGG - Chr8:88864618-88864637 MsG0880047622.01.T01:CDS 30.0%
!!! AGCAGCTTTTTTCGAAATAA+TGG + Chr8:88864390-88864409 None:intergenic 30.0%
!!! ATCCATTTTTTTTGGATCCA+TGG + Chr8:88863676-88863695 None:intergenic 30.0%
!!! CAAACTATTTTGAAGCAAAC+TGG + Chr8:88864600-88864619 None:intergenic 30.0%
!!! TCCATTTTTTTTGGATCCAT+GGG + Chr8:88863675-88863694 None:intergenic 30.0%
!!! TTTTCAAGTGACCACATAAT+TGG + Chr8:88864550-88864569 None:intergenic 30.0%
AAAAGATAAGAAGCTGTCTC+GGG - Chr8:88862519-88862538 MsG0880047622.01.T01:intron 35.0%
AAACGACAACTTAGAAGATG+AGG + Chr8:88866070-88866089 None:intergenic 35.0%
AGAAAACTCAACTCTCTTGT+TGG + Chr8:88864519-88864538 None:intergenic 35.0%
AGGCATAAGCAACATTGAAA+CGG - Chr8:88864977-88864996 MsG0880047622.01.T01:intron 35.0%
AGTGCGCAAATTGAATTAAG+TGG - Chr8:88862744-88862763 MsG0880047622.01.T01:intron 35.0%
ATCTTCCTGGGAAGTAAAAA+AGG + Chr8:88865021-88865040 None:intergenic 35.0%
ATGATTCCTGGTTTACTAAG+CGG - Chr8:88863412-88863431 MsG0880047622.01.T01:intron 35.0%
ATGTCCACCAAAACAGAAAA+TGG + Chr8:88865580-88865599 None:intergenic 35.0%
ATGTGGAAAAGATTGGGAAT+TGG - Chr8:88865331-88865350 MsG0880047622.01.T01:intron 35.0%
ATTTCCACACCAACTAGAAT+AGG + Chr8:88862978-88862997 None:intergenic 35.0%
CAATTCTTTCAAACCCTCAA+TGG + Chr8:88864067-88864086 None:intergenic 35.0%
CATTCACAAGTCCTTAAAAG+TGG + Chr8:88864632-88864651 None:intergenic 35.0%
CTATAAGTATTAGTTGCAGG+CGG - Chr8:88863749-88863768 MsG0880047622.01.T01:intron 35.0%
CTTTACTTTAGTTGAGCTCT+CGG - Chr8:88865943-88865962 MsG0880047622.01.T01:intron 35.0%
GAAAACTCAACTCTCTTGTT+GGG + Chr8:88864518-88864537 None:intergenic 35.0%
GAACTTTCAACAAGATTCCA+AGG - Chr8:88862153-88862172 MsG0880047622.01.T01:CDS 35.0%
GATGTCAACGTTGCAAAAAT+GGG - Chr8:88865629-88865648 MsG0880047622.01.T01:intron 35.0%
GCCTGCAACTAATACTTATA+GGG + Chr8:88863750-88863769 None:intergenic 35.0%
TAAAATTTCCATGCTAGGTG+AGG + Chr8:88863900-88863919 None:intergenic 35.0%
TAAATCGTTGTTGTAGGAGT+TGG - Chr8:88861920-88861939 MsG0880047622.01.T01:three_prime_UTR 35.0%
TAGGAAAATGATTGTCGTTG+GGG - Chr8:88865793-88865812 MsG0880047622.01.T01:intron 35.0%
TATTAGGAAACCATGATTCC+TGG - Chr8:88863400-88863419 MsG0880047622.01.T01:intron 35.0%
TCAACCACATTTGCATCTTA+TGG + Chr8:88865606-88865625 None:intergenic 35.0%
TCCCATGGATCCAAAAAAAA+TGG - Chr8:88863671-88863690 MsG0880047622.01.T01:intron 35.0%
TCTAAATCCTTTCCCATTGA+GGG - Chr8:88864051-88864070 MsG0880047622.01.T01:intron 35.0%
TCTATTCCTTTGGTTGTGTT+CGG - Chr8:88865734-88865753 MsG0880047622.01.T01:intron 35.0%
TCTTTCTTCTGTTCGAATTG+TGG - Chr8:88861997-88862016 MsG0880047622.01.T01:CDS 35.0%
TGATGTCAACGTTGCAAAAA+TGG - Chr8:88865628-88865647 MsG0880047622.01.T01:intron 35.0%
TGTACCATAAGATGCAAATG+TGG - Chr8:88865599-88865618 MsG0880047622.01.T01:intron 35.0%
TGTGTGTTAAATTTACGTGG+TGG - Chr8:88863485-88863504 MsG0880047622.01.T01:intron 35.0%
TTCATACTAGATATGCCATC+AGG - Chr8:88862573-88862592 MsG0880047622.01.T01:intron 35.0%
TTCTAAATCCTTTCCCATTG+AGG - Chr8:88864050-88864069 MsG0880047622.01.T01:intron 35.0%
TTGAAACGGTAAAGTGAAGT+TGG - Chr8:88864991-88865010 MsG0880047622.01.T01:intron 35.0%
! AAGTATAGTAGTGATTTGCC+TGG - Chr8:88865554-88865573 MsG0880047622.01.T01:intron 35.0%
! ATAAAAGTAAGTGTCGTTGG+TGG - Chr8:88864339-88864358 MsG0880047622.01.T01:intron 35.0%
! TAAAAGTAAGTGTCGTTGGT+GGG - Chr8:88864340-88864359 MsG0880047622.01.T01:intron 35.0%
! TCTGGTTCGAATGATATATG+TGG - Chr8:88865314-88865333 MsG0880047622.01.T01:intron 35.0%
!! AATCTAGAAAGGTGCCAATT+AGG + Chr8:88864184-88864203 None:intergenic 35.0%
!! ATCTTTTTAAGCATGACTGC+TGG - Chr8:88862131-88862150 MsG0880047622.01.T01:CDS 35.0%
!! TTAGTGTTGTTGGTTCTTTG+TGG - Chr8:88862952-88862971 MsG0880047622.01.T01:intron 35.0%
!!! TCTATGCTGCTTTTTAACTG+TGG - Chr8:88864094-88864113 MsG0880047622.01.T01:intron 35.0%
AACGGTAAAGTGAAGTTGGA+TGG - Chr8:88864995-88865014 MsG0880047622.01.T01:intron 40.0%
AAGTCCTATTTCCTCACTAG+AGG - Chr8:88865901-88865920 MsG0880047622.01.T01:intron 40.0%
AGAAAACCGCTTAGTAAACC+AGG + Chr8:88863421-88863440 None:intergenic 40.0%
AGAAGAAGAAGAAGAAGACG+CGG + Chr8:88861980-88861999 None:intergenic 40.0%
AGAATCCAGAAAGCTGCTAT+TGG - Chr8:88865152-88865171 MsG0880047622.01.T01:intron 40.0%
ATTACTGTGAATCTCAGCCT+TGG - Chr8:88864276-88864295 MsG0880047622.01.T01:intron 40.0%
ATTTCCTCACTAGAGGCAAA+TGG - Chr8:88865908-88865927 MsG0880047622.01.T01:intron 40.0%
CAAAAGCTATGCTATGCTCT+TGG - Chr8:88862251-88862270 MsG0880047622.01.T01:CDS 40.0%
CGAGAGCTCAACTAAAGTAA+AGG + Chr8:88865945-88865964 None:intergenic 40.0%
CGCCTGCAACTAATACTTAT+AGG + Chr8:88863751-88863770 None:intergenic 40.0%
CTTCCCAGGAAGATAATGTA+TGG - Chr8:88865027-88865046 MsG0880047622.01.T01:intron 40.0%
CTTGTGAACGTGACATCAAA+AGG + Chr8:88862359-88862378 None:intergenic 40.0%
GAACGTGACATCAAAAGGAA+GGG + Chr8:88862354-88862373 None:intergenic 40.0%
GATGTGCAAAAACAATGCCA+TGG + Chr8:88863035-88863054 None:intergenic 40.0%
GCCCTATAAGTATTAGTTGC+AGG - Chr8:88863746-88863765 MsG0880047622.01.T01:intron 40.0%
GCTTAGTAAACCAGGAATCA+TGG + Chr8:88863413-88863432 None:intergenic 40.0%
GGAAAAGATTGGGAATTGGA+TGG - Chr8:88865335-88865354 MsG0880047622.01.T01:intron 40.0%
GGGTATCAGTTATTGACGAT+AGG - Chr8:88865996-88866015 MsG0880047622.01.T01:intron 40.0%
TACAATGGCAACTTCAGTAG+TGG + Chr8:88865060-88865079 None:intergenic 40.0%
TACAGTCAAGTAACTCTAGG+TGG + Chr8:88864939-88864958 None:intergenic 40.0%
TACCAGAGTTGCAGAAAGTT+GGG - Chr8:88865976-88865995 MsG0880047622.01.T01:intron 40.0%
TATCATTCGAACCAGAACCA+AGG + Chr8:88865310-88865329 None:intergenic 40.0%
TCTGTTCGAATTGTGGTTGT+CGG - Chr8:88862004-88862023 MsG0880047622.01.T01:CDS 40.0%
TCTTTGTGGCCTATTCTAGT+TGG - Chr8:88862966-88862985 MsG0880047622.01.T01:intron 40.0%
TGAACGTGACATCAAAAGGA+AGG + Chr8:88862355-88862374 None:intergenic 40.0%
TGCATAGATGAAACAGTTCG+AGG - Chr8:88866114-88866133 MsG0880047622.01.T01:five_prime_UTR 40.0%
TTACCAGAGTTGCAGAAAGT+TGG - Chr8:88865975-88865994 MsG0880047622.01.T01:intron 40.0%
TTTCAAACCCTCAATGGGAA+AGG + Chr8:88864061-88864080 None:intergenic 40.0%
TTTGCCTCTAGTGAGGAAAT+AGG + Chr8:88865908-88865927 None:intergenic 40.0%
! ACGCTTTTCATTTAGGGAGA+GGG + Chr8:88863621-88863640 None:intergenic 40.0%
! ACTTTTGACCCTAGGAACAA+TGG + Chr8:88865846-88865865 None:intergenic 40.0%
! CATCAAGAGTGTACTCATCA+TGG + Chr8:88863931-88863950 None:intergenic 40.0%
! GGCTGATAGTGCTAGATATT+GGG + Chr8:88864918-88864937 None:intergenic 40.0%
! TCTAGTTGGTGTGGAAATAG+CGG - Chr8:88862980-88862999 MsG0880047622.01.T01:intron 40.0%
! TGGCTGATAGTGCTAGATAT+TGG + Chr8:88864919-88864938 None:intergenic 40.0%
! TTACTAAGCGGTTTTCTGAC+AGG - Chr8:88863424-88863443 MsG0880047622.01.T01:intron 40.0%
! TTCCTGAATTTTCTTCGTGC+AGG - Chr8:88865284-88865303 MsG0880047622.01.T01:intron 40.0%
!! CAAGAGCATAGCATAGCTTT+TGG + Chr8:88862253-88862272 None:intergenic 40.0%
!! TTTGTTAGGTGATTTTGGCG+AGG - Chr8:88863323-88863342 MsG0880047622.01.T01:intron 40.0%
AATCTCTTCTCTAGCAGTGC+TGG - Chr8:88865236-88865255 MsG0880047622.01.T01:intron 45.0%
AATTGTGGTTGTCGGTGATG+TGG - Chr8:88862012-88862031 MsG0880047622.01.T01:CDS 45.0%
ACTTTAGTTGAGCTCTCGGA+AGG - Chr8:88865947-88865966 MsG0880047622.01.T01:intron 45.0%
AGATTCTGTGTTGCACCTGT+GGG + Chr8:88865221-88865240 None:intergenic 45.0%
AGCAGCTTTCTGGATTCTCT+TGG + Chr8:88865150-88865169 None:intergenic 45.0%
CACCAAAACAGAAAATGGCC+AGG + Chr8:88865575-88865594 None:intergenic 45.0%
CGAGGACATGAGAAGCTTAA+AGG - Chr8:88865874-88865893 MsG0880047622.01.T01:CDS 45.0%
GAAAAAAGCTGCTGTCTGCA+AGG - Chr8:88864397-88864416 MsG0880047622.01.T01:intron 45.0%
GAGTTCAGCTACAGCTAGAA+TGG - Chr8:88863829-88863848 MsG0880047622.01.T01:intron 45.0%
GATTGGGAATTGGATGGTGA+TGG - Chr8:88865341-88865360 MsG0880047622.01.T01:intron 45.0%
GGCATCAGGATGTAATCGTA+AGG + Chr8:88863879-88863898 None:intergenic 45.0%
GGTTTACCCCCACATGAAAA+TGG + Chr8:88864369-88864388 None:intergenic 45.0%
GTGTACCAATAGCAGCTTTC+TGG + Chr8:88865160-88865179 None:intergenic 45.0%
TACCCAACTTTCTGCAACTC+TGG + Chr8:88865981-88866000 None:intergenic 45.0%
TGTACCATTTGCCTCTAGTG+AGG + Chr8:88865915-88865934 None:intergenic 45.0%
! AATTTGGAGTGGGGGAAATC+AGG + Chr8:88865475-88865494 None:intergenic 45.0%
! AATTTTCTTCGTGCAGGCCT+TGG - Chr8:88865290-88865309 MsG0880047622.01.T01:intron 45.0%
! CACGCTTTTCATTTAGGGAG+AGG + Chr8:88863622-88863641 None:intergenic 45.0%
! CGCTTTTCATTTAGGGAGAG+GGG + Chr8:88863620-88863639 None:intergenic 45.0%
! CTAGTTGGTGTGGAAATAGC+GGG - Chr8:88862981-88863000 MsG0880047622.01.T01:intron 45.0%
! GCGTGCACGCTTTTCATTTA+GGG + Chr8:88863627-88863646 None:intergenic 45.0%
! GCTTTGTTGCTCGACTTCAA+TGG - Chr8:88861951-88861970 MsG0880047622.01.T01:CDS 45.0%
! GCTTTTCATTTAGGGAGAGG+GGG + Chr8:88863619-88863638 None:intergenic 45.0%
! TGCGTGCACGCTTTTCATTT+AGG + Chr8:88863628-88863647 None:intergenic 45.0%
!! CTGGCCATTTTCTGTTTTGG+TGG - Chr8:88865573-88865592 MsG0880047622.01.T01:intron 45.0%
!! TGCCTGGCCATTTTCTGTTT+TGG - Chr8:88865570-88865589 MsG0880047622.01.T01:intron 45.0%
!! TTCACGCGCCTACCTTATTT+TGG - Chr8:88861896-88861915 MsG0880047622.01.T01:three_prime_UTR 45.0%
!! TTGAAGCAAACTGGCTCTGA+TGG + Chr8:88864591-88864610 None:intergenic 45.0%
!! TTGGATGGTGATGGTGATCA+TGG - Chr8:88865350-88865369 MsG0880047622.01.T01:intron 45.0%
!!! GATGGCCTTTTTTACTTCCC+AGG - Chr8:88865013-88865032 MsG0880047622.01.T01:intron 45.0%
AAAGAGCTCGCACATGGCAA+GGG - Chr8:88865767-88865786 MsG0880047622.01.T01:intron 50.0%
AGAAGAAGAAGAAGACGCGG+CGG + Chr8:88861977-88861996 None:intergenic 50.0%
AGCTATGCTATGCTCTTGGC+TGG - Chr8:88862255-88862274 MsG0880047622.01.T01:CDS 50.0%
AGCTGCTATTGGTACACCAG+AGG - Chr8:88865163-88865182 MsG0880047622.01.T01:intron 50.0%
ATGCACAAAGAGCTCGCACA+TGG - Chr8:88865761-88865780 MsG0880047622.01.T01:intron 50.0%
ATGTGGCCGAACACAACCAA+AGG + Chr8:88865743-88865762 None:intergenic 50.0%
CGTGCACGCATACACATTCA+CGG - Chr8:88863638-88863657 MsG0880047622.01.T01:intron 50.0%
GAATGGGGCCATTGTTCCTA+GGG - Chr8:88865835-88865854 MsG0880047622.01.T01:intron 50.0%
GAGATTCTGTGTTGCACCTG+TGG + Chr8:88865222-88865241 None:intergenic 50.0%
GCTATGCTATGCTCTTGGCT+GGG - Chr8:88862256-88862275 MsG0880047622.01.T01:CDS 50.0%
GGCCTGCACGAAGAAAATTC+AGG + Chr8:88865289-88865308 None:intergenic 50.0%
GTCTGGCTGCAGTGCAATAT+AGG + Chr8:88862855-88862874 None:intergenic 50.0%
TATTGCACTGCAGCCAGACT+TGG - Chr8:88862856-88862875 MsG0880047622.01.T01:CDS 50.0%
TGAATGGGGCCATTGTTCCT+AGG - Chr8:88865834-88865853 MsG0880047622.01.T01:intron 50.0%
TGTGAATCTCAGCCTTGGCA+AGG - Chr8:88864281-88864300 MsG0880047622.01.T01:intron 50.0%
! AAGTAAGTGTCGTTGGTGGG+CGG - Chr8:88864343-88864362 MsG0880047622.01.T01:intron 50.0%
! CTGGAGGAATTGAAGTGCCT+TGG + Chr8:88862173-88862192 None:intergenic 50.0%
! GTGGCCTATTCTAGTTGGTG+TGG - Chr8:88862971-88862990 MsG0880047622.01.T01:intron 50.0%
! TCGCACATGGCAAGGGTTTT+AGG - Chr8:88865774-88865793 MsG0880047622.01.T01:intron 50.0%
!! CCAGACTTGGTGCTCTTTAC+AGG - Chr8:88862869-88862888 MsG0880047622.01.T01:CDS 50.0%
!! CCTGTAAAGAGCACCAAGTC+TGG + Chr8:88862872-88862891 None:intergenic 50.0%
!! CTTGCACATAATGGACCCAC+AGG - Chr8:88865203-88865222 MsG0880047622.01.T01:intron 50.0%
CAAAGAGCTCGCACATGGCA+AGG - Chr8:88865766-88865785 MsG0880047622.01.T01:intron 55.0%
GATGGTGATCATGGTGACCC+AGG - Chr8:88865359-88865378 MsG0880047622.01.T01:intron 55.0%
GCACGCATACACATTCACGG+AGG - Chr8:88863641-88863660 MsG0880047622.01.T01:intron 55.0%
GTGCGAGCTCTTTGTGCATG+TGG + Chr8:88865760-88865779 None:intergenic 55.0%
TCCTGATGCCTCACCTAGCA+TGG - Chr8:88863889-88863908 MsG0880047622.01.T01:intron 55.0%
! TCCATGCTAGGTGAGGCATC+AGG + Chr8:88863893-88863912 None:intergenic 55.0%
!! GTGGGCGGCCATTTTCATGT+GGG - Chr8:88864358-88864377 MsG0880047622.01.T01:intron 55.0%
!! TGGGCGGCCATTTTCATGTG+GGG - Chr8:88864359-88864378 MsG0880047622.01.T01:intron 55.0%
AAGCCACGTGCTCCTTGCCA+AGG + Chr8:88864296-88864315 None:intergenic 60.0%
CACGGAGGCAGACAATCCCA+TGG - Chr8:88863656-88863675 MsG0880047622.01.T01:intron 60.0%
CTTCGTGCAGGCCTTGGTTC+TGG - Chr8:88865296-88865315 MsG0880047622.01.T01:intron 60.0%
!! GGGCGGCCATTTTCATGTGG+GGG - Chr8:88864360-88864379 MsG0880047622.01.T01:intron 60.0%
!! GGTGGGCGGCCATTTTCATG+TGG - Chr8:88864357-88864376 MsG0880047622.01.T01:intron 60.0%
! CAGCCTTGGCAAGGAGCACG+TGG - Chr8:88864290-88864309 MsG0880047622.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 88861870 88866190 88861870 ID=MsG0880047622.01;Name=MsG0880047622.01
Chr8 mRNA 88861870 88866190 88861870 ID=MsG0880047622.01.T01;Parent=MsG0880047622.01;Name=MsG0880047622.01.T01;_AED=0.45;_eAED=0.45;_QI=159|0.6|0.83|1|0.6|0.66|6|70|260
Chr8 exon 88861870 88862370 88861870 ID=MsG0880047622.01.T01:exon:2407;Parent=MsG0880047622.01.T01
Chr8 exon 88862680 88862754 88862680 ID=MsG0880047622.01.T01:exon:2406;Parent=MsG0880047622.01.T01
Chr8 exon 88862836 88862942 88862836 ID=MsG0880047622.01.T01:exon:2405;Parent=MsG0880047622.01.T01
Chr8 exon 88864615 88864729 88864615 ID=MsG0880047622.01.T01:exon:2404;Parent=MsG0880047622.01.T01
Chr8 exon 88865869 88865918 88865869 ID=MsG0880047622.01.T01:exon:2403;Parent=MsG0880047622.01.T01
Chr8 exon 88866027 88866190 88866027 ID=MsG0880047622.01.T01:exon:2402;Parent=MsG0880047622.01.T01
Chr8 five_prime_UTR 88866032 88866190 88866032 ID=MsG0880047622.01.T01:five_prime_utr;Parent=MsG0880047622.01.T01
Chr8 CDS 88866027 88866031 88866027 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 CDS 88865869 88865918 88865869 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 CDS 88864615 88864729 88864615 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 CDS 88862836 88862942 88862836 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 CDS 88862680 88862754 88862680 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 CDS 88861940 88862370 88861940 ID=MsG0880047622.01.T01:cds;Parent=MsG0880047622.01.T01
Chr8 three_prime_UTR 88861870 88861939 88861870 ID=MsG0880047622.01.T01:three_prime_utr;Parent=MsG0880047622.01.T01
Gene Sequence

>MsG0880047622.01.T01

ATGTGCATGACTGCTGGAACTTTCAACAAGATTCCAAGGCACTTCAATTCCTCCAGTGATTTTGGCGAGGAAAATCTTGAAATCGTTCGCAGTGTTGCAAATATTGAATTTGCTAAAGCAGTTATATTAGGAAACCATGATTCCTGGTTTACTAAGCGGTTTTCTGACAGATATGGAGTTAAAGACATGGATCAGAGTGCCAAGAGAATCCAGAAAGCTGCTATTGGTACACCAGAGGATCATTTTCTGATATTACTTGCACATAATGGACCCACAGGCCTTGGTTCTGGTTCGAATGATATATGTGGAAAAGATTGGGAATTGGATGGTGATGGTGATCATGGTGACCCAGATCTAGCACACGCAATATCCTTGCTAAAAGAGAATAATCAGGTATCTATTCCTTTGGTTGTGTTCGGCCACATGCACAAAGAGCTCGCACATGGCAAGGGTTTTAGGAAAATGATTGTCGTTGGGGACGACAACATCATATACTTGAATGGGGCCATTGTTCCTAGGGTCAAAAGTTTGAGCGACGAGGACATGAGAAGCTTAAAGGTTGAAAGTCCTATTTCCTCACTAGAGGCAAATGGTACAGCAAGAGCCTTTACTTTAGTTGAGCTCTCGGAAGGAAGAGTTACCAGAGTTGCAGAAAGTTGGGTATCAGTTATTGACGATAGGACCACATTAAAAGAACAATATTATTCGAAGGCAATTAAGAATCAAGATCCTCATCTTCTAAGTTGTCGTTTTTACTTAAGTGACATGCTATCTAATGCATAG
Protein sequence

>MsG0880047622.01.T01

MCMTAGTFNKIPRHFNSSSDFGEENLEIVRSVANIEFAKAVILGNHDSWFTKRFSDRYGVKDMDQSAKRIQKAAIGTPEDHFLILLAHNGPTGLGSGSNDICGKDWELDGDGDHGDPDLAHAISLLKENNQVSIPLVVFGHMHKELAHGKGFRKMIVVGDDNIIYLNGAIVPRVKSLSDEDMRSLKVESPISSLEANGTARAFTLVELSEGRVTRVAESWVSVIDDRTTLKEQYYSKAIKNQDPHLLSCRFYLSDMLSNA*