Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047690.01.T01 | KEH32652.1 | 84 | 75 | 3 | 1 | 11 | 85 | 96 | 161 | 2.57E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047690.01.T01 | Q8GYK2 | 60.256 | 78 | 22 | 1 | 5 | 82 | 188 | 256 | 9.25E-27 | 101 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047690.01.T01 | A0A072USL3 | 84.000 | 75 | 3 | 1 | 11 | 85 | 96 | 161 | 1.23e-34 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010423.01 | MsG0880047690.01 | 0.829943 | 3.879551e-55 | 4.029421e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047690.01.T01 | MTR_4g132890 | 84.000 | 75 | 3 | 1 | 11 | 85 | 96 | 161 | 3.11e-38 | 125 |
| MsG0880047690.01.T01 | MTR_4g132890 | 84.000 | 75 | 3 | 1 | 11 | 85 | 150 | 215 | 2.19e-37 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047690.01.T01 | AT4G22300 | 60.256 | 78 | 22 | 1 | 5 | 82 | 188 | 256 | 9.43e-28 | 101 |
| MsG0880047690.01.T01 | AT4G22305 | 55.294 | 85 | 29 | 1 | 1 | 85 | 139 | 214 | 2.18e-26 | 97.1 |
| MsG0880047690.01.T01 | AT1G18773 | 67.568 | 37 | 12 | 0 | 12 | 48 | 25 | 61 | 4.74e-13 | 58.5 |
| MsG0880047690.01.T01 | AT1G18773 | 64.865 | 37 | 13 | 0 | 12 | 48 | 25 | 61 | 1.26e-12 | 57.4 |
| MsG0880047690.01.T01 | AT1G18773 | 66.667 | 36 | 12 | 0 | 12 | 47 | 25 | 60 | 2.01e-12 | 57.4 |
| MsG0880047690.01.T01 | AT1G18773 | 66.667 | 36 | 12 | 0 | 12 | 47 | 25 | 60 | 2.54e-12 | 56.6 |
Find 26 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTGTTAGTTGCGAGTTTA+AGG | 0.172548 | 8:+89650976 | MsG0880047690.01.T01:CDS |
| ATTCCAAGTTCCCTATAATT+TGG | 0.179172 | 8:+89651003 | MsG0880047690.01.T01:CDS |
| TGTTGTTCACAATATTTAAT+AGG | 0.260593 | 8:+89651277 | MsG0880047690.01.T01:three_prime_UTR |
| AGAGATACAGATTGAAAATT+AGG | 0.267362 | 8:-89651172 | None:intergenic |
| CAGCCCATGGGACCATAGTA+TGG | 0.302977 | 8:-89650894 | None:intergenic |
| TGGTATTATTAGGCTTATCC+TGG | 0.441301 | 8:+89651075 | MsG0880047690.01.T01:intron |
| CTCCTCGTTGTTTATTGAGT+GGG | 0.455255 | 8:-89651103 | None:intergenic |
| TCTTGATCTCATGTCATTGT+GGG | 0.469043 | 8:+89651308 | MsG0880047690.01.T01:three_prime_UTR |
| AGCTAAAGCATCTTGAGTCT+TGG | 0.470537 | 8:+89651124 | MsG0880047690.01.T01:CDS |
| TAACCAAATTATAGGGAACT+TGG | 0.470901 | 8:-89651006 | None:intergenic |
| CATACTATGGTCCCATGGGC+TGG | 0.472438 | 8:+89650895 | MsG0880047690.01.T01:CDS |
| TTATACAGACACCCATACTA+TGG | 0.480186 | 8:+89650882 | MsG0880047690.01.T01:CDS |
| TTTATTGAGTGGGCAAGACC+AGG | 0.534942 | 8:-89651093 | None:intergenic |
| GCTCCTCGTTGTTTATTGAG+TGG | 0.540846 | 8:-89651104 | None:intergenic |
| TTCTTGATCTCATGTCATTG+TGG | 0.550457 | 8:+89651307 | MsG0880047690.01.T01:three_prime_UTR |
| CACCCATACTATGGTCCCAT+GGG | 0.572624 | 8:+89650891 | MsG0880047690.01.T01:CDS |
| GATACAGATTGAAAATTAGG+AGG | 0.576640 | 8:-89651169 | None:intergenic |
| AAAACAGTATTGTTCGAGGC+AGG | 0.580046 | 8:+89650923 | MsG0880047690.01.T01:CDS |
| AGCCCATGGGACCATAGTAT+GGG | 0.591504 | 8:-89650893 | None:intergenic |
| ACACCCATACTATGGTCCCA+TGG | 0.599983 | 8:+89650890 | MsG0880047690.01.T01:CDS |
| TGACAAAACAGTATTGTTCG+AGG | 0.612436 | 8:+89650919 | MsG0880047690.01.T01:CDS |
| GAAAATAGAGAAAGTCATAG+AGG | 0.619475 | 8:-89651250 | None:intergenic |
| AAAATTAGGAGGAACTCTGT+AGG | 0.620221 | 8:-89651158 | None:intergenic |
| TATAACATTGTGTACATACC+AGG | 0.635579 | 8:-89650864 | None:intergenic |
| GAGAAAGTCATAGAGGTCAG+CGG | 0.643692 | 8:-89651243 | None:intergenic |
| TGCCCACTCAATAAACAACG+AGG | 0.786416 | 8:+89651101 | MsG0880047690.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAAATAACCAAATTATA+GGG | - | Chr8:89651016-89651035 | None:intergenic | 15.0% |
| !! | TGAAAAAATAACCAAATTAT+AGG | - | Chr8:89651017-89651036 | None:intergenic | 15.0% |
| !! | TATTGACATTTGGTATTATT+AGG | + | Chr8:89651065-89651084 | MsG0880047690.01.T01:intron | 20.0% |
| !! | TGTTGTTCACAATATTTAAT+AGG | + | Chr8:89651277-89651296 | MsG0880047690.01.T01:three_prime_UTR | 20.0% |
| ! | ACCAAATGTCAATATTGATA+TGG | - | Chr8:89651059-89651078 | None:intergenic | 25.0% |
| ! | AGAGATACAGATTGAAAATT+AGG | - | Chr8:89651175-89651194 | None:intergenic | 25.0% |
| ! | TCCATATCAATATTGACATT+TGG | + | Chr8:89651055-89651074 | MsG0880047690.01.T01:intron | 25.0% |
| !!! | AACTAACACCAATTTTTTCA+AGG | - | Chr8:89650967-89650986 | None:intergenic | 25.0% |
| ATTCCAAGTTCCCTATAATT+TGG | + | Chr8:89651003-89651022 | MsG0880047690.01.T01:CDS | 30.0% | |
| ATTGATATGGACAGAATTGT+AGG | - | Chr8:89651046-89651065 | None:intergenic | 30.0% | |
| GAAAATAGAGAAAGTCATAG+AGG | - | Chr8:89651253-89651272 | None:intergenic | 30.0% | |
| GATACAGATTGAAAATTAGG+AGG | - | Chr8:89651172-89651191 | None:intergenic | 30.0% | |
| TAACCAAATTATAGGGAACT+TGG | - | Chr8:89651009-89651028 | None:intergenic | 30.0% | |
| TATAACATTGTGTACATACC+AGG | - | Chr8:89650867-89650886 | None:intergenic | 30.0% | |
| TTGATATGGACAGAATTGTA+GGG | - | Chr8:89651045-89651064 | None:intergenic | 30.0% | |
| !!! | AATTTTTTCAAGGAAAGGAG+GGG | - | Chr8:89650957-89650976 | None:intergenic | 30.0% |
| !!! | ACACCAATTTTTTCAAGGAA+AGG | - | Chr8:89650962-89650981 | None:intergenic | 30.0% |
| !!! | CAATTTTTTCAAGGAAAGGA+GGG | - | Chr8:89650958-89650977 | None:intergenic | 30.0% |
| AAAATTAGGAGGAACTCTGT+AGG | - | Chr8:89651161-89651180 | None:intergenic | 35.0% | |
| CCTCCTTTCCTTGAAAAAAT+TGG | + | Chr8:89650956-89650975 | MsG0880047690.01.T01:CDS | 35.0% | |
| TCTTGATCTCATGTCATTGT+GGG | + | Chr8:89651308-89651327 | MsG0880047690.01.T01:three_prime_UTR | 35.0% | |
| TGACAAAACAGTATTGTTCG+AGG | + | Chr8:89650919-89650938 | MsG0880047690.01.T01:CDS | 35.0% | |
| TGATATGGACAGAATTGTAG+GGG | - | Chr8:89651044-89651063 | None:intergenic | 35.0% | |
| TGGTATTATTAGGCTTATCC+TGG | + | Chr8:89651075-89651094 | MsG0880047690.01.T01:intron | 35.0% | |
| TTATACAGACACCCATACTA+TGG | + | Chr8:89650882-89650901 | MsG0880047690.01.T01:CDS | 35.0% | |
| TTCTTGATCTCATGTCATTG+TGG | + | Chr8:89651307-89651326 | MsG0880047690.01.T01:three_prime_UTR | 35.0% | |
| !!! | CCAATTTTTTCAAGGAAAGG+AGG | - | Chr8:89650959-89650978 | None:intergenic | 35.0% |
| AAAACAGTATTGTTCGAGGC+AGG | + | Chr8:89650923-89650942 | MsG0880047690.01.T01:CDS | 40.0% | |
| CTCCTCGTTGTTTATTGAGT+GGG | - | Chr8:89651106-89651125 | None:intergenic | 40.0% | |
| ! | AGCTAAAGCATCTTGAGTCT+TGG | + | Chr8:89651124-89651143 | MsG0880047690.01.T01:CDS | 40.0% |
| ! | TGGTGTTAGTTGCGAGTTTA+AGG | + | Chr8:89650976-89650995 | MsG0880047690.01.T01:CDS | 40.0% |
| GAGAAAGTCATAGAGGTCAG+CGG | - | Chr8:89651246-89651265 | None:intergenic | 45.0% | |
| GCTCCTCGTTGTTTATTGAG+TGG | - | Chr8:89651107-89651126 | None:intergenic | 45.0% | |
| TGCCCACTCAATAAACAACG+AGG | + | Chr8:89651101-89651120 | MsG0880047690.01.T01:CDS | 45.0% | |
| TTTATTGAGTGGGCAAGACC+AGG | - | Chr8:89651096-89651115 | None:intergenic | 45.0% | |
| ACACCCATACTATGGTCCCA+TGG | + | Chr8:89650890-89650909 | MsG0880047690.01.T01:CDS | 50.0% | |
| AGCCCATGGGACCATAGTAT+GGG | - | Chr8:89650896-89650915 | None:intergenic | 50.0% | |
| CACCCATACTATGGTCCCAT+GGG | + | Chr8:89650891-89650910 | MsG0880047690.01.T01:CDS | 50.0% | |
| ! | TGTTTTGTCAGCCAGCCCAT+GGG | - | Chr8:89650909-89650928 | None:intergenic | 50.0% |
| CAGCCCATGGGACCATAGTA+TGG | - | Chr8:89650897-89650916 | None:intergenic | 55.0% | |
| CATACTATGGTCCCATGGGC+TGG | + | Chr8:89650895-89650914 | MsG0880047690.01.T01:CDS | 55.0% | |
| ! | CTGTTTTGTCAGCCAGCCCA+TGG | - | Chr8:89650910-89650929 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 89650857 | 89651359 | 89650857 | ID=MsG0880047690.01;Name=MsG0880047690.01 |
| Chr8 | mRNA | 89650857 | 89651359 | 89650857 | ID=MsG0880047690.01.T01;Parent=MsG0880047690.01;Name=MsG0880047690.01.T01;_AED=0.46;_eAED=0.53;_QI=0|0|0.5|1|0|0|2|183|85 |
| Chr8 | exon | 89650857 | 89651024 | 89650857 | ID=MsG0880047690.01.T01:exon:2534;Parent=MsG0880047690.01.T01 |
| Chr8 | exon | 89651087 | 89651359 | 89651087 | ID=MsG0880047690.01.T01:exon:2535;Parent=MsG0880047690.01.T01 |
| Chr8 | CDS | 89650857 | 89651024 | 89650857 | ID=MsG0880047690.01.T01:cds;Parent=MsG0880047690.01.T01 |
| Chr8 | CDS | 89651087 | 89651176 | 89651087 | ID=MsG0880047690.01.T01:cds;Parent=MsG0880047690.01.T01 |
| Chr8 | three_prime_UTR | 89651177 | 89651359 | 89651177 | ID=MsG0880047690.01.T01:three_prime_utr;Parent=MsG0880047690.01.T01 |
| Gene Sequence |
| Protein sequence |
