Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000520 | Msa1130240 | 0.868372 | 6.856054e-66 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTCTTGCTTTGCTGTGC+TGG | 0.223155 | 1_1:-1680686 | Msa0000520:CDS |
| CGGCTATTGGTGCTTCATTT+GGG | 0.240735 | 1_1:-1680708 | Msa0000520:CDS |
| TCGGCTATTGGTGCTTCATT+TGG | 0.261418 | 1_1:-1680709 | Msa0000520:CDS |
| TTTGGATGTGGCTCGGCTAT+TGG | 0.297657 | 1_1:-1680721 | Msa0000520:CDS |
| GTTTCTTGCTTTGCTGTGCT+GGG | 0.321557 | 1_1:-1680685 | Msa0000520:CDS |
| ATATGTGGAGCAACCTCATT+TGG | 0.344422 | 1_1:-1680739 | Msa0000520:CDS |
| CGGTCTCTGTATCTGTTTGT+GGG | 0.434430 | 1_1:-1680549 | Msa0000520:three_prime_UTR |
| GTCCTTAGTCCTTCAATATG+TGG | 0.441394 | 1_1:-1680754 | Msa0000520:CDS |
| ATTGCTGCTGTGTTGACATC+AGG | 0.460585 | 1_1:-1680525 | Msa0000520:three_prime_UTR |
| CACCAAACCGCAACAGCCAC+CGG | 0.470881 | 1_1:+1680465 | None:intergenic |
| GCGGTCTCTGTATCTGTTTG+TGG | 0.482483 | 1_1:-1680550 | Msa0000520:three_prime_UTR |
| GAGGTTGCTCCACATATTGA+AGG | 0.515151 | 1_1:+1680745 | None:intergenic |
| TTTCTTGCTTTGCTGTGCTG+GGG | 0.517631 | 1_1:-1680684 | Msa0000520:CDS |
| CTCCACATATTGAAGGACTA+AGG | 0.519079 | 1_1:+1680752 | None:intergenic |
| GGCTATTGGTGCTTCATTTG+GGG | 0.535223 | 1_1:-1680707 | Msa0000520:CDS |
| AACCTCATTTGGATGTGGCT+CGG | 0.543359 | 1_1:-1680728 | Msa0000520:CDS |
| AGCCGAGCCACATCCAAATG+AGG | 0.589740 | 1_1:+1680726 | None:intergenic |
| AAAACTTATTTAGAAGACAA+TGG | 0.613147 | 1_1:-1680141 | None:intergenic |
| TTTGTTGCCGGTGGCTGTTG+CGG | 0.617996 | 1_1:-1680472 | Msa0000520:intron |
| GGAGCAACCTCATTTGGATG+TGG | 0.632398 | 1_1:-1680733 | Msa0000520:CDS |
| GTTGACATCAGGTTGTGCAG+TGG | 0.680238 | 1_1:-1680514 | Msa0000520:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACATATCCTAATTAATATT+TGG | - | chr1_1:1680687-1680706 | Msa0000520:CDS | 15.0% |
| !!! | AAACTATGTTTTTTTGTTTT+AGG | - | chr1_1:1680742-1680761 | Msa0000520:CDS | 15.0% |
| !!! | GGTTTTTTTTAGAGTTATTT+TGG | - | chr1_1:1680558-1680577 | Msa0000520:three_prime_UTR | 20.0% |
| !! | ATATCCTAATTAATATTTGG+AGG | - | chr1_1:1680690-1680709 | Msa0000520:CDS | 20.0% |
| ! | TATATAATTAGCATCTCCAA+AGG | + | chr1_1:1680720-1680739 | None:intergenic | 25.0% |
| TGTTATGGAATTAGGACTAT+AGG | - | chr1_1:1680537-1680556 | Msa0000520:three_prime_UTR | 30.0% | |
| TCCTAATTAATATTTGGAGG+TGG | - | chr1_1:1680693-1680712 | Msa0000520:CDS | 30.0% | |
| ACCACCTCCAAATATTAATT+AGG | + | chr1_1:1680697-1680716 | None:intergenic | 30.0% | |
| !!! | GCTTTCTGTTGTTTTAGCTT+TGG | - | chr1_1:1680264-1680283 | Msa0000520:intron | 35.0% |
| ! | CAGTTTGAGGGTTTTTGATA+GGG | - | chr1_1:1680311-1680330 | Msa0000520:intron | 35.0% |
| ! | AGTTTGAGGGTTTTTGATAG+GGG | - | chr1_1:1680312-1680331 | Msa0000520:intron | 35.0% |
| TCTTTCAGATGGGATCAAAT+CGG | + | chr1_1:1680613-1680632 | None:intergenic | 35.0% | |
| TTGAGACACTTCTTTCAGAT+GGG | + | chr1_1:1680623-1680642 | None:intergenic | 35.0% | |
| ATTGAGACACTTCTTTCAGA+TGG | + | chr1_1:1680624-1680643 | None:intergenic | 35.0% | |
| !! | ATCTGAAAGAAGTGTCTCAA+TGG | - | chr1_1:1680623-1680642 | Msa0000520:CDS | 35.0% |
| !! | TCTGAAAGAAGTGTCTCAAT+GGG | - | chr1_1:1680624-1680643 | Msa0000520:CDS | 35.0% |
| GTCCTTAGTCCTTCAATATG+TGG | - | chr1_1:1680153-1680172 | Msa0000520:intron | 40.0% | |
| CTCCACATATTGAAGGACTA+AGG | + | chr1_1:1680158-1680177 | None:intergenic | 40.0% | |
| ATATGTGGAGCAACCTCATT+TGG | - | chr1_1:1680168-1680187 | Msa0000520:intron | 40.0% | |
| !!! | GATGTCATGTGTTTTAGTGC+TGG | - | chr1_1:1680288-1680307 | Msa0000520:intron | 40.0% |
| !!! | TTTTAGTGCTGGCAGTTTGA+GGG | - | chr1_1:1680299-1680318 | Msa0000520:intron | 40.0% |
| ! | GCAGTTTGAGGGTTTTTGAT+AGG | - | chr1_1:1680310-1680329 | Msa0000520:intron | 40.0% |
| ! | GTTTGAGGGTTTTTGATAGG+GGG | - | chr1_1:1680313-1680332 | Msa0000520:intron | 40.0% |
| !! | ATGGTGTTGTGTTTGTTGTG+TGG | - | chr1_1:1680490-1680509 | Msa0000520:three_prime_UTR | 40.0% |
| CTTAGTTCCTGCTACTGTTA+TGG | - | chr1_1:1680522-1680541 | Msa0000520:three_prime_UTR | 40.0% | |
| CCTAATTCCATAACAGTAGC+AGG | + | chr1_1:1680532-1680551 | None:intergenic | 40.0% | |
| CCTGCTACTGTTATGGAATT+AGG | - | chr1_1:1680529-1680548 | Msa0000520:three_prime_UTR | 40.0% | |
| AATATTTGGAGGTGGTCCTT+TGG | - | chr1_1:1680701-1680720 | Msa0000520:CDS | 40.0% | |
| GAGGTTGCTCCACATATTGA+AGG | + | chr1_1:1680165-1680184 | None:intergenic | 45.0% | |
| AACCTCATTTGGATGTGGCT+CGG | - | chr1_1:1680179-1680198 | Msa0000520:intron | 45.0% | |
| ! | TCGGCTATTGGTGCTTCATT+TGG | - | chr1_1:1680198-1680217 | Msa0000520:intron | 45.0% |
| ! | CGGCTATTGGTGCTTCATTT+GGG | - | chr1_1:1680199-1680218 | Msa0000520:intron | 45.0% |
| ! | GGCTATTGGTGCTTCATTTG+GGG | - | chr1_1:1680200-1680219 | Msa0000520:intron | 45.0% |
| ! | GTTTCTTGCTTTGCTGTGCT+GGG | - | chr1_1:1680222-1680241 | Msa0000520:intron | 45.0% |
| ! | TTTCTTGCTTTGCTGTGCTG+GGG | - | chr1_1:1680223-1680242 | Msa0000520:intron | 45.0% |
| !!! | CTGGGGTTTTTTGAGTTTGC+TGG | - | chr1_1:1680240-1680259 | Msa0000520:intron | 45.0% |
| !!! | GTTTTAGTGCTGGCAGTTTG+AGG | - | chr1_1:1680298-1680317 | Msa0000520:intron | 45.0% |
| ! | GATAGGGGGTTGTGTTTTCT+AGG | - | chr1_1:1680327-1680346 | Msa0000520:intron | 45.0% |
| CGGTCTCTGTATCTGTTTGT+GGG | - | chr1_1:1680358-1680377 | Msa0000520:intron | 45.0% | |
| ! | ATTGCTGCTGTGTTGACATC+AGG | - | chr1_1:1680382-1680401 | Msa0000520:intron | 45.0% |
| !!! | TGTTGTGCTGCTTTTGTTGC+CGG | - | chr1_1:1680423-1680442 | Msa0000520:intron | 45.0% |
| !!! | TTGCGGTTTGGTGTTTTGGT+AGG | - | chr1_1:1680452-1680471 | Msa0000520:intron | 45.0% |
| GGAGCAACCTCATTTGGATG+TGG | - | chr1_1:1680174-1680193 | Msa0000520:intron | 50.0% | |
| ! | TTTGGATGTGGCTCGGCTAT+TGG | - | chr1_1:1680186-1680205 | Msa0000520:intron | 50.0% |
| ! | GGTTTCTTGCTTTGCTGTGC+TGG | - | chr1_1:1680221-1680240 | Msa0000520:intron | 50.0% |
| !! | GTGTTTTCTAGGTGCTGCAG+CGG | - | chr1_1:1680338-1680357 | Msa0000520:intron | 50.0% |
| GCGGTCTCTGTATCTGTTTG+TGG | - | chr1_1:1680357-1680376 | Msa0000520:intron | 50.0% | |
| ! | GTTGACATCAGGTTGTGCAG+TGG | - | chr1_1:1680393-1680412 | Msa0000520:intron | 50.0% |
| !!! | GCTGTTGCGGTTTGGTGTTT+TGG | - | chr1_1:1680448-1680467 | Msa0000520:intron | 50.0% |
| !!! | CGGTTTGGTGTTTTGGTAGG+AGG | - | chr1_1:1680455-1680474 | Msa0000520:intron | 50.0% |
| !! | GTTTTGGTAGGAGGCATGTC+AGG | - | chr1_1:1680464-1680483 | Msa0000520:intron | 50.0% |
| !! | TAGGAGGCATGTCAGGTTGA+TGG | - | chr1_1:1680471-1680490 | Msa0000520:intron | 50.0% |
| AGCCGAGCCACATCCAAATG+AGG | + | chr1_1:1680184-1680203 | None:intergenic | 55.0% | |
| !!! | TGTGCTGCTTTTGTTGCCGG+TGG | - | chr1_1:1680426-1680445 | Msa0000520:intron | 55.0% |
| TTTGTTGCCGGTGGCTGTTG+CGG | - | chr1_1:1680435-1680454 | Msa0000520:intron | 55.0% | |
| CACCAAACCGCAACAGCCAC+CGG | + | chr1_1:1680445-1680464 | None:intergenic | 60.0% | |
| TGCCGGTGGCTGTTGCGGTT+TGG | - | chr1_1:1680440-1680459 | Msa0000520:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 1680143 | 1680786 | 1680143 | ID=Msa0000520;Name=Msa0000520 |
| chr1_1 | mRNA | 1680143 | 1680786 | 1680143 | ID=Msa0000520-mRNA-1;Parent=Msa0000520;Name=Msa0000520-mRNA-1;_AED=0.38;_eAED=0.76;_QI=5|0|0|1|0|0|3|118|56 |
| chr1_1 | exon | 1680609 | 1680786 | 1680609 | ID=Msa0000520-mRNA-1:exon:258;Parent=Msa0000520-mRNA-1 |
| chr1_1 | exon | 1680473 | 1680580 | 1680473 | ID=Msa0000520-mRNA-1:exon:257;Parent=Msa0000520-mRNA-1 |
| chr1_1 | exon | 1680143 | 1680150 | 1680143 | ID=Msa0000520-mRNA-1:exon:256;Parent=Msa0000520-mRNA-1 |
| chr1_1 | five_prime_UTR | 1680782 | 1680786 | 1680782 | ID=Msa0000520-mRNA-1:five_prime_utr;Parent=Msa0000520-mRNA-1 |
| chr1_1 | CDS | 1680611 | 1680781 | 1680611 | ID=Msa0000520-mRNA-1:cds;Parent=Msa0000520-mRNA-1 |
| chr1_1 | three_prime_UTR | 1680609 | 1680610 | 1680609 | ID=Msa0000520-mRNA-1:three_prime_utr;Parent=Msa0000520-mRNA-1 |
| chr1_1 | three_prime_UTR | 1680473 | 1680580 | 1680473 | ID=Msa0000520-mRNA-1:three_prime_utr;Parent=Msa0000520-mRNA-1 |
| chr1_1 | three_prime_UTR | 1680143 | 1680150 | 1680143 | ID=Msa0000520-mRNA-1:three_prime_utr;Parent=Msa0000520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
