AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0006030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006030 MtrunA17_Chr1g0150081 96.216 185 7 0 1 185 1 185 6.58e-132 367
Msa0006030 MtrunA17_Chr7g0244371 56.085 189 73 5 1 184 18 201 3.69e-65 199
Msa0006030 MtrunA17_Chr7g0244321 54.787 188 74 6 4 185 22 204 5.32e-61 188
Msa0006030 MtrunA17_Chr7g0244331 54.255 188 75 6 4 185 22 204 7.17e-60 186
Msa0006030 MtrunA17_Chr7g0244341 52.880 191 79 5 1 185 19 204 8.26e-60 186
Msa0006030 MtrunA17_Chr7g0244361 49.246 199 77 5 1 184 43 232 2.00e-55 175
Msa0006030 MtrunA17_Chr4g0013291 42.857 189 93 6 3 185 13 192 9.49e-43 141
Msa0006030 MtrunA17_Chr1g0150091 59.836 122 32 2 80 185 11 131 2.88e-40 133
Msa0006030 MtrunA17_Chr1g0150101 74.684 79 20 0 107 185 1 79 8.14e-35 117
Msa0006030 MtrunA17_Chr7g0244111 54.630 108 45 2 81 184 8 115 1.65e-34 118
Msa0006030 MtrunA17_Chr4g0003271 23.656 186 133 6 5 185 7 188 5.63e-12 62.0
Msa0006030 MtrunA17_Chr5g0432221 30.435 184 99 8 17 182 37 209 9.37e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006030 AT5G01750.2 40.526 190 102 6 2 185 24 208 2.85e-46 151
Msa0006030 AT3G11740.1 44.974 189 92 7 4 185 2 185 2.49e-45 148
Msa0006030 AT2G14560.1 38.172 186 104 5 5 184 7 187 5.34e-37 127
Msa0006030 AT1G33840.1 35.979 189 110 5 2 184 24 207 2.73e-36 126
Msa0006030 AT2G14560.3 38.172 186 104 5 5 184 67 247 5.60e-36 126
Msa0006030 AT5G01750.1 39.869 153 84 5 2 151 24 171 1.17e-35 123
Msa0006030 AT3G16900.1 35.519 183 96 6 5 185 15 177 7.29e-33 116
Msa0006030 AT2G14560.2 36.842 152 88 4 5 153 7 153 2.49e-28 104
Msa0006030 AT1G33840.2 35.484 155 92 4 2 153 24 173 4.09e-28 103
Msa0006030 AT2G14560.4 36.842 152 88 4 5 153 67 213 2.64e-27 103
Msa0006030 AT3G56180.1 30.890 191 118 6 2 185 15 198 9.07e-25 95.9
Msa0006030 AT3G56180.2 31.088 193 117 7 2 185 15 200 6.51e-24 94.0
Msa0006030 AT3G10986.1 29.381 194 120 5 4 185 3 191 1.17e-21 87.8

Find 41 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATATTGAGGGCTGATTA+TGG 0.310803 1_1:-8812996 None:intergenic
AATATTAGAGATGTGTTTCT+TGG 0.319557 1_1:-8812937 None:intergenic
CAAATTTATCTCAACAATCT+TGG 0.324672 1_1:+8813578 Msa0006030:CDS
TGAATGCATAATCGATGTTT+GGG 0.334963 1_1:-8813779 None:intergenic
ATGAATGCATAATCGATGTT+TGG 0.340520 1_1:-8813780 None:intergenic
CGGTTCTTAGTTGATGCTAA+TGG 0.343712 1_1:+8813273 Msa0006030:CDS
CTAGTAAAGATAAGATCATT+TGG 0.367319 1_1:-8813466 None:intergenic
AACCTGAGCAACTACGTTGT+TGG 0.404004 1_1:-8813620 None:intergenic
TTCTAGCAGCAGATGATTGT+TGG 0.405704 1_1:+8813416 Msa0006030:CDS
TCTTGTAGGTTGTTTAATCA+AGG 0.409735 1_1:-8813821 None:intergenic
CAAATGATCTTATCTTTACT+AGG 0.420505 1_1:+8813467 Msa0006030:CDS
TGTTAGCAGGGAAAAGTTCA+TGG 0.426125 1_1:+8813746 Msa0006030:CDS
TGTTGTGATTATCAAATCAA+TGG 0.429127 1_1:-8813148 None:intergenic
AACTAAGAACCGGTGTTGGC+GGG 0.442279 1_1:-8813262 None:intergenic
TGATATTAATTCAATCATCA+TGG 0.466431 1_1:+8812967 Msa0006030:exon
CGATCTACGGAGATGAAGAA+TGG 0.473284 1_1:-8813301 None:intergenic
AGCCCTCAATATTGTGCTCC+AGG 0.475888 1_1:+8813004 Msa0006030:CDS
GTTCATCATTGATGCAACTA+AGG 0.493660 1_1:+8813494 Msa0006030:CDS
CATTAGCATCAACTAAGAAC+CGG 0.495060 1_1:-8813272 None:intergenic
AGCGTGGCAATATTGTTAGC+AGG 0.495716 1_1:+8813733 Msa0006030:CDS
ATCCAACAACGTAGTTGCTC+AGG 0.499631 1_1:+8813618 Msa0006030:CDS
CAACTAAGAACCGGTGTTGG+CGG 0.506342 1_1:-8813263 None:intergenic
CTTGTAGGTTGTTTAATCAA+GGG 0.509070 1_1:-8813820 None:intergenic
TTGATGAACGTACCGATCTA+CGG 0.520939 1_1:-8813314 None:intergenic
TACCTGGAGCACAATATTGA+GGG 0.535069 1_1:-8813006 None:intergenic
GATCTACGGAGATGAAGAAT+GGG 0.541516 1_1:-8813300 None:intergenic
ATACCTGGAGCACAATATTG+AGG 0.542310 1_1:-8813007 None:intergenic
CAATCTTGGAATGTTTACAT+TGG 0.552539 1_1:+8813592 Msa0006030:CDS
CATTCTTCATCTCCGTAGAT+CGG 0.556024 1_1:+8813302 Msa0006030:CDS
GATTGTTGGAAAGCATATAG+AGG 0.563993 1_1:+8813430 Msa0006030:CDS
GTTGTGATTATCAAATCAAT+GGG 0.566325 1_1:-8813147 None:intergenic
ACTAAGAACCGGTGTTGGCG+GGG 0.574675 1_1:-8813261 None:intergenic
GACTATGAGAGATAACTTCA+CGG 0.579627 1_1:+8813176 Msa0006030:CDS
CATCAACTAAGAACCGGTGT+TGG 0.588205 1_1:-8813266 None:intergenic
TCGTAACACCCCGCCAACAC+CGG 0.589651 1_1:+8813253 Msa0006030:CDS
GCGTGGCAATATTGTTAGCA+GGG 0.615963 1_1:+8813734 Msa0006030:CDS
CATCGATTATGCATTCATTG+TGG 0.620012 1_1:+8813785 Msa0006030:CDS
GTGTTGGCGGGGTGTTACGA+TGG 0.625287 1_1:-8813250 None:intergenic
TTGCAGATAAATAAGAAGCG+TGG 0.668205 1_1:+8813717 Msa0006030:intron
GAATGCATAATCGATGTTTG+GGG 0.710689 1_1:-8813778 None:intergenic
ATTTGATAATCACAACAGAG+AGG 0.729502 1_1:+8813154 Msa0006030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCAAATAAGTTTTAATTA+AGG - chr1_1:8813066-8813085 None:intergenic 10.0%
!!! AATCAAATAAGTTTTAATTA+AGG - chr1_1:8813066-8813085 None:intergenic 10.0%
!!! TATCCTTTTAACAATTTTTT+TGG + chr1_1:8813103-8813122 Msa0006030:intron 15.0%
!! AAACCAAAAAAATTGTTAAA+AGG - chr1_1:8813109-8813128 None:intergenic 15.0%
!!! TATCCTTTTAACAATTTTTT+TGG + chr1_1:8813103-8813122 Msa0006030:intron 15.0%
!! AAACCAAAAAAATTGTTAAA+AGG - chr1_1:8813109-8813128 None:intergenic 15.0%
!! TGATATTAATTCAATCATCA+TGG + chr1_1:8812967-8812986 Msa0006030:exon 20.0%
!!! AGATTTTTGCAAAAAAGAAA+TGG + chr1_1:8813654-8813673 Msa0006030:intron 20.0%
!! TGATATTAATTCAATCATCA+TGG + chr1_1:8812967-8812986 Msa0006030:exon 20.0%
!!! AGATTTTTGCAAAAAAGAAA+TGG + chr1_1:8813654-8813673 Msa0006030:intron 20.0%
! AATATTAGAGATGTGTTTCT+TGG - chr1_1:8812940-8812959 None:intergenic 25.0%
! GTTGTGATTATCAAATCAAT+GGG - chr1_1:8813150-8813169 None:intergenic 25.0%
! TGTTGTGATTATCAAATCAA+TGG - chr1_1:8813151-8813170 None:intergenic 25.0%
! ATGTGTTTCTTAGTTGAAAA+TGG + chr1_1:8813345-8813364 Msa0006030:intron 25.0%
! CTAGTAAAGATAAGATCATT+TGG - chr1_1:8813469-8813488 None:intergenic 25.0%
! CAAATGATCTTATCTTTACT+AGG + chr1_1:8813467-8813486 Msa0006030:CDS 25.0%
!! GACCAAATTAAATGTGTTTT+TGG + chr1_1:8813516-8813535 Msa0006030:CDS 25.0%
! TGCCAAAAACACATTTAATT+TGG - chr1_1:8813521-8813540 None:intergenic 25.0%
! CAAATTTATCTCAACAATCT+TGG + chr1_1:8813578-8813597 Msa0006030:CDS 25.0%
!! ATAATCACAGTTATTGGTTA+TGG - chr1_1:8813698-8813717 None:intergenic 25.0%
! AATATTAGAGATGTGTTTCT+TGG - chr1_1:8812940-8812959 None:intergenic 25.0%
! GTTGTGATTATCAAATCAAT+GGG - chr1_1:8813150-8813169 None:intergenic 25.0%
! TGTTGTGATTATCAAATCAA+TGG - chr1_1:8813151-8813170 None:intergenic 25.0%
! ATGTGTTTCTTAGTTGAAAA+TGG + chr1_1:8813345-8813364 Msa0006030:intron 25.0%
! CTAGTAAAGATAAGATCATT+TGG - chr1_1:8813469-8813488 None:intergenic 25.0%
! CAAATGATCTTATCTTTACT+AGG + chr1_1:8813467-8813486 Msa0006030:CDS 25.0%
!! GACCAAATTAAATGTGTTTT+TGG + chr1_1:8813516-8813535 Msa0006030:CDS 25.0%
! TGCCAAAAACACATTTAATT+TGG - chr1_1:8813521-8813540 None:intergenic 25.0%
! CAAATTTATCTCAACAATCT+TGG + chr1_1:8813578-8813597 Msa0006030:CDS 25.0%
!! ATAATCACAGTTATTGGTTA+TGG - chr1_1:8813698-8813717 None:intergenic 25.0%
AAATTGTTCCGAAAAGATGA+TGG - chr1_1:8813036-8813055 None:intergenic 30.0%
! ATTTGATAATCACAACAGAG+AGG + chr1_1:8813154-8813173 Msa0006030:CDS 30.0%
!! TTTTTGGCAAATAACAACAC+AGG + chr1_1:8813532-8813551 Msa0006030:CDS 30.0%
CAATCTTGGAATGTTTACAT+TGG + chr1_1:8813592-8813611 Msa0006030:CDS 30.0%
TCTGCAATAATCACAGTTAT+TGG - chr1_1:8813704-8813723 None:intergenic 30.0%
TGAATGCATAATCGATGTTT+GGG - chr1_1:8813782-8813801 None:intergenic 30.0%
ATGAATGCATAATCGATGTT+TGG - chr1_1:8813783-8813802 None:intergenic 30.0%
AAATTGTTCCGAAAAGATGA+TGG - chr1_1:8813036-8813055 None:intergenic 30.0%
! ATTTGATAATCACAACAGAG+AGG + chr1_1:8813154-8813173 Msa0006030:CDS 30.0%
!! TTTTTGGCAAATAACAACAC+AGG + chr1_1:8813532-8813551 Msa0006030:CDS 30.0%
CAATCTTGGAATGTTTACAT+TGG + chr1_1:8813592-8813611 Msa0006030:CDS 30.0%
TCTGCAATAATCACAGTTAT+TGG - chr1_1:8813704-8813723 None:intergenic 30.0%
TGAATGCATAATCGATGTTT+GGG - chr1_1:8813782-8813801 None:intergenic 30.0%
ATGAATGCATAATCGATGTT+TGG - chr1_1:8813783-8813802 None:intergenic 30.0%
ACAATATTGAGGGCTGATTA+TGG - chr1_1:8812999-8813018 None:intergenic 35.0%
! AAAAGATGATGGTTCATACC+TGG - chr1_1:8813025-8813044 None:intergenic 35.0%
! GGTATGAACCATCATCTTTT+CGG + chr1_1:8813025-8813044 Msa0006030:intron 35.0%
GACTATGAGAGATAACTTCA+CGG + chr1_1:8813176-8813195 Msa0006030:CDS 35.0%
CATTAGCATCAACTAAGAAC+CGG - chr1_1:8813275-8813294 None:intergenic 35.0%
! GATTGTTGGAAAGCATATAG+AGG + chr1_1:8813430-8813449 Msa0006030:CDS 35.0%
GTTCATCATTGATGCAACTA+AGG + chr1_1:8813494-8813513 Msa0006030:CDS 35.0%
TTGCAGATAAATAAGAAGCG+TGG + chr1_1:8813717-8813736 Msa0006030:intron 35.0%
GAATGCATAATCGATGTTTG+GGG - chr1_1:8813781-8813800 None:intergenic 35.0%
! CATCGATTATGCATTCATTG+TGG + chr1_1:8813785-8813804 Msa0006030:CDS 35.0%
ACAATATTGAGGGCTGATTA+TGG - chr1_1:8812999-8813018 None:intergenic 35.0%
! AAAAGATGATGGTTCATACC+TGG - chr1_1:8813025-8813044 None:intergenic 35.0%
! GGTATGAACCATCATCTTTT+CGG + chr1_1:8813025-8813044 Msa0006030:intron 35.0%
GACTATGAGAGATAACTTCA+CGG + chr1_1:8813176-8813195 Msa0006030:CDS 35.0%
CATTAGCATCAACTAAGAAC+CGG - chr1_1:8813275-8813294 None:intergenic 35.0%
! GATTGTTGGAAAGCATATAG+AGG + chr1_1:8813430-8813449 Msa0006030:CDS 35.0%
GTTCATCATTGATGCAACTA+AGG + chr1_1:8813494-8813513 Msa0006030:CDS 35.0%
TTGCAGATAAATAAGAAGCG+TGG + chr1_1:8813717-8813736 Msa0006030:intron 35.0%
GAATGCATAATCGATGTTTG+GGG - chr1_1:8813781-8813800 None:intergenic 35.0%
! CATCGATTATGCATTCATTG+TGG + chr1_1:8813785-8813804 Msa0006030:CDS 35.0%
TACCTGGAGCACAATATTGA+GGG - chr1_1:8813009-8813028 None:intergenic 40.0%
ATACCTGGAGCACAATATTG+AGG - chr1_1:8813010-8813029 None:intergenic 40.0%
! CGGTTCTTAGTTGATGCTAA+TGG + chr1_1:8813273-8813292 Msa0006030:CDS 40.0%
GATCTACGGAGATGAAGAAT+GGG - chr1_1:8813303-8813322 None:intergenic 40.0%
CATTCTTCATCTCCGTAGAT+CGG + chr1_1:8813302-8813321 Msa0006030:CDS 40.0%
! TTGATGAACGTACCGATCTA+CGG - chr1_1:8813317-8813336 None:intergenic 40.0%
TTCTAGCAGCAGATGATTGT+TGG + chr1_1:8813416-8813435 Msa0006030:CDS 40.0%
TGTTAGCAGGGAAAAGTTCA+TGG + chr1_1:8813746-8813765 Msa0006030:CDS 40.0%
TACCTGGAGCACAATATTGA+GGG - chr1_1:8813009-8813028 None:intergenic 40.0%
ATACCTGGAGCACAATATTG+AGG - chr1_1:8813010-8813029 None:intergenic 40.0%
! CGGTTCTTAGTTGATGCTAA+TGG + chr1_1:8813273-8813292 Msa0006030:CDS 40.0%
GATCTACGGAGATGAAGAAT+GGG - chr1_1:8813303-8813322 None:intergenic 40.0%
CATTCTTCATCTCCGTAGAT+CGG + chr1_1:8813302-8813321 Msa0006030:CDS 40.0%
! TTGATGAACGTACCGATCTA+CGG - chr1_1:8813317-8813336 None:intergenic 40.0%
TTCTAGCAGCAGATGATTGT+TGG + chr1_1:8813416-8813435 Msa0006030:CDS 40.0%
TGTTAGCAGGGAAAAGTTCA+TGG + chr1_1:8813746-8813765 Msa0006030:CDS 40.0%
CATCAACTAAGAACCGGTGT+TGG - chr1_1:8813269-8813288 None:intergenic 45.0%
CGATCTACGGAGATGAAGAA+TGG - chr1_1:8813304-8813323 None:intergenic 45.0%
ATCCAACAACGTAGTTGCTC+AGG + chr1_1:8813618-8813637 Msa0006030:CDS 45.0%
AACCTGAGCAACTACGTTGT+TGG - chr1_1:8813623-8813642 None:intergenic 45.0%
AGCGTGGCAATATTGTTAGC+AGG + chr1_1:8813733-8813752 Msa0006030:CDS 45.0%
GCGTGGCAATATTGTTAGCA+GGG + chr1_1:8813734-8813753 Msa0006030:CDS 45.0%
CATCAACTAAGAACCGGTGT+TGG - chr1_1:8813269-8813288 None:intergenic 45.0%
CGATCTACGGAGATGAAGAA+TGG - chr1_1:8813304-8813323 None:intergenic 45.0%
ATCCAACAACGTAGTTGCTC+AGG + chr1_1:8813618-8813637 Msa0006030:CDS 45.0%
AACCTGAGCAACTACGTTGT+TGG - chr1_1:8813623-8813642 None:intergenic 45.0%
AGCGTGGCAATATTGTTAGC+AGG + chr1_1:8813733-8813752 Msa0006030:CDS 45.0%
GCGTGGCAATATTGTTAGCA+GGG + chr1_1:8813734-8813753 Msa0006030:CDS 45.0%
AGCCCTCAATATTGTGCTCC+AGG + chr1_1:8813004-8813023 Msa0006030:CDS 50.0%
!! AACTAAGAACCGGTGTTGGC+GGG - chr1_1:8813265-8813284 None:intergenic 50.0%
! CAACTAAGAACCGGTGTTGG+CGG - chr1_1:8813266-8813285 None:intergenic 50.0%
AGCCCTCAATATTGTGCTCC+AGG + chr1_1:8813004-8813023 Msa0006030:CDS 50.0%
!! AACTAAGAACCGGTGTTGGC+GGG - chr1_1:8813265-8813284 None:intergenic 50.0%
! CAACTAAGAACCGGTGTTGG+CGG - chr1_1:8813266-8813285 None:intergenic 50.0%
!! ACTAAGAACCGGTGTTGGCG+GGG - chr1_1:8813264-8813283 None:intergenic 55.0%
!! ACTAAGAACCGGTGTTGGCG+GGG - chr1_1:8813264-8813283 None:intergenic 55.0%
! GTGTTGGCGGGGTGTTACGA+TGG - chr1_1:8813253-8813272 None:intergenic 60.0%
TCGTAACACCCCGCCAACAC+CGG + chr1_1:8813253-8813272 Msa0006030:CDS 60.0%
! GTGTTGGCGGGGTGTTACGA+TGG - chr1_1:8813253-8813272 None:intergenic 60.0%
TCGTAACACCCCGCCAACAC+CGG + chr1_1:8813253-8813272 Msa0006030:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 8812901 8813830 8812901 ID=Msa0006030;Name=Msa0006030
chr1_1 mRNA 8812901 8813830 8812901 ID=Msa0006030-mRNA-1;Parent=Msa0006030;Name=Msa0006030-mRNA-1;_AED=0.12;_eAED=0.12;_QI=85|0.66|0.25|1|1|1|4|0|185
chr1_1 exon 8812901 8813025 8812901 ID=Msa0006030-mRNA-1:exon:3126;Parent=Msa0006030-mRNA-1
chr1_1 exon 8813139 8813323 8813139 ID=Msa0006030-mRNA-1:exon:3127;Parent=Msa0006030-mRNA-1
chr1_1 exon 8813415 8813639 8813415 ID=Msa0006030-mRNA-1:exon:3128;Parent=Msa0006030-mRNA-1
chr1_1 exon 8813723 8813830 8813723 ID=Msa0006030-mRNA-1:exon:3129;Parent=Msa0006030-mRNA-1
chr1_1 five_prime_UTR 8812901 8812985 8812901 ID=Msa0006030-mRNA-1:five_prime_utr;Parent=Msa0006030-mRNA-1
chr1_1 CDS 8812986 8813025 8812986 ID=Msa0006030-mRNA-1:cds;Parent=Msa0006030-mRNA-1
chr1_1 CDS 8813139 8813323 8813139 ID=Msa0006030-mRNA-1:cds;Parent=Msa0006030-mRNA-1
chr1_1 CDS 8813415 8813639 8813415 ID=Msa0006030-mRNA-1:cds;Parent=Msa0006030-mRNA-1
chr1_1 CDS 8813723 8813830 8813723 ID=Msa0006030-mRNA-1:cds;Parent=Msa0006030-mRNA-1
Gene Sequence

>Msa0006030

ATGGCAAATGCCATAATCAGCCCTCAATATTGTGCTCCAGCTACACATCCCATTGATTTGATAATCACAACAGAGAGGACTATGAGAGATAACTTCACGGTCAGAGACATCAACGACAACATCGTTTTCACAGTCAAAAGTTCTCTTGTAACCATCGTAACACCCCGCCAACACCGGTTCTTAGTTGATGCTAATGGAAATCCCATTCTTCATCTCCGTAGATCGCTTCTAGCAGCAGATGATTGTTGGAAAGCATATAGAGGTGAAAGCACAGAGCCAAATGATCTTATCTTTACTAGGAAACGTTCATCATTGATGCAACTAAGGACCAAATTAAATGTGTTTTTGGCAAATAACAACACAGGAGTTTGTGATTTCACTGTCAAAGCAAATTTATCTCAACAATCTTGGAATGTTTACATTGGTGAATCCAACAACGTAGTTGCTCAGATAAATAAGAAGCGTGGCAATATTGTTAGCAGGGAAAAGTTCATGGTCACTGTGTCCCCAAACATCGATTATGCATTCATTGTGGCTCTAATTGTGACCCTTGATTAA

Protein sequence

>Msa0006030

MANAIISPQYCAPATHPIDLIITTERTMRDNFTVRDINDNIVFTVKSSLVTIVTPRQHRFLVDANGNPILHLRRSLLAADDCWKAYRGESTEPNDLIFTRKRSSLMQLRTKLNVFLANNNTGVCDFTVKANLSQQSWNVYIGESNNVVAQINKKRGNIVSREKFMVTVSPNIDYAFIVALIVTLD*