Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012310 | Msa0707630 | 0.961630 | 6.324158e-120 | -8.615850e-47 |
| Msa0012310 | Msa0708150 | 0.947829 | 3.132620e-106 | -8.615850e-47 |
| Msa0012310 | Msa1026930 | 0.988775 | 2.325016e-175 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0012310 | MtrunA17_Chr8g0359031 | 47.059 | 119 | 61 | 1 | 43 | 159 | 38 | 156 | 8.47e-36 | 122 |
| Msa0012310 | MtrunA17_Chr5g0393511 | 45.000 | 120 | 65 | 1 | 43 | 162 | 38 | 156 | 1.18e-31 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATATTGCATGTCAAATTT+TGG | 0.111779 | 1_1:-18521915 | None:intergenic |
| CAATTGCTATGTGAAATTTC+TGG | 0.218719 | 1_1:+18522354 | Msa0012310:CDS |
| TGCAGTAGGTGTGCAATTGC+AGG | 0.348444 | 1_1:+18521624 | Msa0012310:five_prime_UTR |
| AAGGAAGGTTACTATATTCC+TGG | 0.378391 | 1_1:+18521879 | Msa0012310:CDS |
| TAAGAGTTACAAACAATTCA+TGG | 0.378550 | 1_1:+18521973 | Msa0012310:CDS |
| AGCATTCTTCTTCTGAATCA+TGG | 0.388381 | 1_1:-18521842 | None:intergenic |
| CATGCAATATGATGACTATA+AGG | 0.392912 | 1_1:+18521926 | Msa0012310:CDS |
| ATTTCGACTCCTATGTGTAT+AGG | 0.431470 | 1_1:-18522127 | None:intergenic |
| AGACATGGAGGTACCGGCTC+TGG | 0.478033 | 1_1:-18521951 | None:intergenic |
| ACACTTACAACAGCATAAGT+TGG | 0.503451 | 1_1:-18522026 | None:intergenic |
| AGGAATATAGTAACCTTCCT+TGG | 0.507376 | 1_1:-18521877 | None:intergenic |
| GATAAGATGAAGAGGTACAT+AGG | 0.508544 | 1_1:+18522247 | Msa0012310:CDS |
| GTAATGTGGAAGTTGATGAT+AGG | 0.517091 | 1_1:+18522299 | Msa0012310:CDS |
| AATTTCACATAGCAATTGAA+AGG | 0.518483 | 1_1:-18522349 | None:intergenic |
| TACCAGTCATTTACCAAGGA+AGG | 0.524931 | 1_1:+18521864 | Msa0012310:CDS |
| TTTGGAATTGGATCAGAACC+AGG | 0.529513 | 1_1:-18521897 | None:intergenic |
| TTGGTTGATTCAGATGAAGA+TGG | 0.537698 | 1_1:+18522184 | Msa0012310:CDS |
| AACCTTCCTTGGTAAATGAC+TGG | 0.545045 | 1_1:-18521866 | None:intergenic |
| CATAGGAGTCGAAATTCTAA+AGG | 0.559600 | 1_1:+18522135 | Msa0012310:CDS |
| ACTCTTAGACATGGAGGTAC+CGG | 0.560718 | 1_1:-18521957 | None:intergenic |
| GAAGCAAATCACACTGACCT+TGG | 0.564869 | 1_1:+18522165 | Msa0012310:CDS |
| TGACTATAAGGAACCAGAGC+CGG | 0.567138 | 1_1:+18521938 | Msa0012310:CDS |
| ATAAGATGAAGAGGTACATA+GGG | 0.570183 | 1_1:+18522248 | Msa0012310:CDS |
| ATGAAGAGGTACATAGGGCA+AGG | 0.571299 | 1_1:+18522253 | Msa0012310:CDS |
| AAGAGTTACAAACAATTCAT+GGG | 0.572552 | 1_1:+18521974 | Msa0012310:CDS |
| CAGCGTATTCCTATACACAT+AGG | 0.575668 | 1_1:+18522118 | Msa0012310:intron |
| CTATGTGAAATTTCTGGAGA+GGG | 0.575789 | 1_1:+18522360 | Msa0012310:CDS |
| TTTGAACAAGACATGATGAC+AGG | 0.594917 | 1_1:-18521696 | None:intergenic |
| TTGTTTGTAACTCTTAGACA+TGG | 0.601947 | 1_1:-18521966 | None:intergenic |
| AGAGGTACATAGGGCAAGGG+TGG | 0.609118 | 1_1:+18522257 | Msa0012310:CDS |
| TCATCATGTCTTGTTCAAAG+AGG | 0.610027 | 1_1:+18521700 | Msa0012310:exon |
| GCTATGTGAAATTTCTGGAG+AGG | 0.612333 | 1_1:+18522359 | Msa0012310:CDS |
| AAGATGGTGATAAGATGAAG+AGG | 0.620620 | 1_1:+18522239 | Msa0012310:CDS |
| TGAAGAGGTACATAGGGCAA+GGG | 0.632528 | 1_1:+18522254 | Msa0012310:CDS |
| TTTGTAACTCTTAGACATGG+AGG | 0.670628 | 1_1:-18521963 | None:intergenic |
| ATCTTCATCTGAATCAACCA+AGG | 0.672333 | 1_1:-18522182 | None:intergenic |
| ATGCTACCAGTCATTTACCA+AGG | 0.679424 | 1_1:+18521860 | Msa0012310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAAGTATTATATTACAAA+TGG | + | chr1_1:18521786-18521805 | Msa0012310:intron | 15.0% |
| !! | TTCAAGTATTATATTACAAA+TGG | + | chr1_1:18521786-18521805 | Msa0012310:intron | 15.0% |
| !!! | TTTTCATCACATAACAAAAT+TGG | + | chr1_1:18521657-18521676 | Msa0012310:five_prime_UTR | 20.0% |
| !! | GTATTATATTACAAATGGAA+TGG | + | chr1_1:18521791-18521810 | Msa0012310:intron | 20.0% |
| !!! | TTTTGTTAAGAAATGTAATG+TGG | + | chr1_1:18522285-18522304 | Msa0012310:CDS | 20.0% |
| !!! | TTTTCATCACATAACAAAAT+TGG | + | chr1_1:18521657-18521676 | Msa0012310:five_prime_UTR | 20.0% |
| !! | GTATTATATTACAAATGGAA+TGG | + | chr1_1:18521791-18521810 | Msa0012310:intron | 20.0% |
| !!! | TTTTGTTAAGAAATGTAATG+TGG | + | chr1_1:18522285-18522304 | Msa0012310:CDS | 20.0% |
| !! | TCATATTGCATGTCAAATTT+TGG | - | chr1_1:18521918-18521937 | None:intergenic | 25.0% |
| ! | TAAGAGTTACAAACAATTCA+TGG | + | chr1_1:18521973-18521992 | Msa0012310:CDS | 25.0% |
| ! | AAGAGTTACAAACAATTCAT+GGG | + | chr1_1:18521974-18521993 | Msa0012310:CDS | 25.0% |
| !!! | GTTGTGTTATTTATAAACAC+TGG | + | chr1_1:18522079-18522098 | Msa0012310:intron | 25.0% |
| ! | GTTATTTATAAACACTGGAT+TGG | + | chr1_1:18522084-18522103 | Msa0012310:intron | 25.0% |
| !!! | TGTGATGTTTTTTTTGAAGA+TGG | + | chr1_1:18522223-18522242 | Msa0012310:CDS | 25.0% |
| ! | AATTTCACATAGCAATTGAA+AGG | - | chr1_1:18522352-18522371 | None:intergenic | 25.0% |
| !! | TCATATTGCATGTCAAATTT+TGG | - | chr1_1:18521918-18521937 | None:intergenic | 25.0% |
| ! | TAAGAGTTACAAACAATTCA+TGG | + | chr1_1:18521973-18521992 | Msa0012310:CDS | 25.0% |
| ! | AAGAGTTACAAACAATTCAT+GGG | + | chr1_1:18521974-18521993 | Msa0012310:CDS | 25.0% |
| !!! | GTTGTGTTATTTATAAACAC+TGG | + | chr1_1:18522079-18522098 | Msa0012310:intron | 25.0% |
| ! | GTTATTTATAAACACTGGAT+TGG | + | chr1_1:18522084-18522103 | Msa0012310:intron | 25.0% |
| !!! | TGTGATGTTTTTTTTGAAGA+TGG | + | chr1_1:18522223-18522242 | Msa0012310:CDS | 25.0% |
| ! | AATTTCACATAGCAATTGAA+AGG | - | chr1_1:18522352-18522371 | None:intergenic | 25.0% |
| ! | TTCTTCTGAATCATGGTTTT+TGG | - | chr1_1:18521838-18521857 | None:intergenic | 30.0% |
| !!! | TGCATGTCAAATTTTGGAAT+TGG | - | chr1_1:18521912-18521931 | None:intergenic | 30.0% |
| CATGCAATATGATGACTATA+AGG | + | chr1_1:18521926-18521945 | Msa0012310:CDS | 30.0% | |
| TTGTTTGTAACTCTTAGACA+TGG | - | chr1_1:18521969-18521988 | None:intergenic | 30.0% | |
| ! | ATGCTGTTGTAAGTGTTTTA+TGG | + | chr1_1:18522033-18522052 | Msa0012310:intron | 30.0% |
| ! | ATAAGATGAAGAGGTACATA+GGG | + | chr1_1:18522248-18522267 | Msa0012310:CDS | 30.0% |
| CAATTGCTATGTGAAATTTC+TGG | + | chr1_1:18522354-18522373 | Msa0012310:CDS | 30.0% | |
| ! | TTCTTCTGAATCATGGTTTT+TGG | - | chr1_1:18521838-18521857 | None:intergenic | 30.0% |
| !!! | TGCATGTCAAATTTTGGAAT+TGG | - | chr1_1:18521912-18521931 | None:intergenic | 30.0% |
| CATGCAATATGATGACTATA+AGG | + | chr1_1:18521926-18521945 | Msa0012310:CDS | 30.0% | |
| TTGTTTGTAACTCTTAGACA+TGG | - | chr1_1:18521969-18521988 | None:intergenic | 30.0% | |
| ! | ATGCTGTTGTAAGTGTTTTA+TGG | + | chr1_1:18522033-18522052 | Msa0012310:intron | 30.0% |
| ! | ATAAGATGAAGAGGTACATA+GGG | + | chr1_1:18522248-18522267 | Msa0012310:CDS | 30.0% |
| CAATTGCTATGTGAAATTTC+TGG | + | chr1_1:18522354-18522373 | Msa0012310:CDS | 30.0% | |
| TTTGAACAAGACATGATGAC+AGG | - | chr1_1:18521699-18521718 | None:intergenic | 35.0% | |
| ! | TCATCATGTCTTGTTCAAAG+AGG | + | chr1_1:18521700-18521719 | Msa0012310:exon | 35.0% |
| AGCATTCTTCTTCTGAATCA+TGG | - | chr1_1:18521845-18521864 | None:intergenic | 35.0% | |
| AGGAATATAGTAACCTTCCT+TGG | - | chr1_1:18521880-18521899 | None:intergenic | 35.0% | |
| AAGGAAGGTTACTATATTCC+TGG | + | chr1_1:18521879-18521898 | Msa0012310:CDS | 35.0% | |
| TTTGTAACTCTTAGACATGG+AGG | - | chr1_1:18521966-18521985 | None:intergenic | 35.0% | |
| ACACTTACAACAGCATAAGT+TGG | - | chr1_1:18522029-18522048 | None:intergenic | 35.0% | |
| ATTTCGACTCCTATGTGTAT+AGG | - | chr1_1:18522130-18522149 | None:intergenic | 35.0% | |
| CATAGGAGTCGAAATTCTAA+AGG | + | chr1_1:18522135-18522154 | Msa0012310:CDS | 35.0% | |
| ATCTTCATCTGAATCAACCA+AGG | - | chr1_1:18522185-18522204 | None:intergenic | 35.0% | |
| ! | TTGGTTGATTCAGATGAAGA+TGG | + | chr1_1:18522184-18522203 | Msa0012310:CDS | 35.0% |
| AAGATGGTGATAAGATGAAG+AGG | + | chr1_1:18522239-18522258 | Msa0012310:CDS | 35.0% | |
| ! | GATAAGATGAAGAGGTACAT+AGG | + | chr1_1:18522247-18522266 | Msa0012310:CDS | 35.0% |
| !! | GTAATGTGGAAGTTGATGAT+AGG | + | chr1_1:18522299-18522318 | Msa0012310:CDS | 35.0% |
| ! | CTATGTGAAATTTCTGGAGA+GGG | + | chr1_1:18522360-18522379 | Msa0012310:CDS | 35.0% |
| TTTGAACAAGACATGATGAC+AGG | - | chr1_1:18521699-18521718 | None:intergenic | 35.0% | |
| ! | TCATCATGTCTTGTTCAAAG+AGG | + | chr1_1:18521700-18521719 | Msa0012310:exon | 35.0% |
| AGCATTCTTCTTCTGAATCA+TGG | - | chr1_1:18521845-18521864 | None:intergenic | 35.0% | |
| AGGAATATAGTAACCTTCCT+TGG | - | chr1_1:18521880-18521899 | None:intergenic | 35.0% | |
| AAGGAAGGTTACTATATTCC+TGG | + | chr1_1:18521879-18521898 | Msa0012310:CDS | 35.0% | |
| TTTGTAACTCTTAGACATGG+AGG | - | chr1_1:18521966-18521985 | None:intergenic | 35.0% | |
| ACACTTACAACAGCATAAGT+TGG | - | chr1_1:18522029-18522048 | None:intergenic | 35.0% | |
| ATTTCGACTCCTATGTGTAT+AGG | - | chr1_1:18522130-18522149 | None:intergenic | 35.0% | |
| CATAGGAGTCGAAATTCTAA+AGG | + | chr1_1:18522135-18522154 | Msa0012310:CDS | 35.0% | |
| ATCTTCATCTGAATCAACCA+AGG | - | chr1_1:18522185-18522204 | None:intergenic | 35.0% | |
| ! | TTGGTTGATTCAGATGAAGA+TGG | + | chr1_1:18522184-18522203 | Msa0012310:CDS | 35.0% |
| AAGATGGTGATAAGATGAAG+AGG | + | chr1_1:18522239-18522258 | Msa0012310:CDS | 35.0% | |
| ! | GATAAGATGAAGAGGTACAT+AGG | + | chr1_1:18522247-18522266 | Msa0012310:CDS | 35.0% |
| !! | GTAATGTGGAAGTTGATGAT+AGG | + | chr1_1:18522299-18522318 | Msa0012310:CDS | 35.0% |
| ! | CTATGTGAAATTTCTGGAGA+GGG | + | chr1_1:18522360-18522379 | Msa0012310:CDS | 35.0% |
| GGAATGGTTGTTCTTAACAG+CGG | + | chr1_1:18521807-18521826 | Msa0012310:intron | 40.0% | |
| GAATGGTTGTTCTTAACAGC+GGG | + | chr1_1:18521808-18521827 | Msa0012310:intron | 40.0% | |
| AATGGTTGTTCTTAACAGCG+GGG | + | chr1_1:18521809-18521828 | Msa0012310:intron | 40.0% | |
| ATGCTACCAGTCATTTACCA+AGG | + | chr1_1:18521860-18521879 | Msa0012310:CDS | 40.0% | |
| TACCAGTCATTTACCAAGGA+AGG | + | chr1_1:18521864-18521883 | Msa0012310:CDS | 40.0% | |
| AACCTTCCTTGGTAAATGAC+TGG | - | chr1_1:18521869-18521888 | None:intergenic | 40.0% | |
| ! | TTTGGAATTGGATCAGAACC+AGG | - | chr1_1:18521900-18521919 | None:intergenic | 40.0% |
| CAGCGTATTCCTATACACAT+AGG | + | chr1_1:18522118-18522137 | Msa0012310:intron | 40.0% | |
| ! | GCTATGTGAAATTTCTGGAG+AGG | + | chr1_1:18522359-18522378 | Msa0012310:CDS | 40.0% |
| GGAATGGTTGTTCTTAACAG+CGG | + | chr1_1:18521807-18521826 | Msa0012310:intron | 40.0% | |
| GAATGGTTGTTCTTAACAGC+GGG | + | chr1_1:18521808-18521827 | Msa0012310:intron | 40.0% | |
| AATGGTTGTTCTTAACAGCG+GGG | + | chr1_1:18521809-18521828 | Msa0012310:intron | 40.0% | |
| ATGCTACCAGTCATTTACCA+AGG | + | chr1_1:18521860-18521879 | Msa0012310:CDS | 40.0% | |
| TACCAGTCATTTACCAAGGA+AGG | + | chr1_1:18521864-18521883 | Msa0012310:CDS | 40.0% | |
| AACCTTCCTTGGTAAATGAC+TGG | - | chr1_1:18521869-18521888 | None:intergenic | 40.0% | |
| ! | TTTGGAATTGGATCAGAACC+AGG | - | chr1_1:18521900-18521919 | None:intergenic | 40.0% |
| CAGCGTATTCCTATACACAT+AGG | + | chr1_1:18522118-18522137 | Msa0012310:intron | 40.0% | |
| ! | GCTATGTGAAATTTCTGGAG+AGG | + | chr1_1:18522359-18522378 | Msa0012310:CDS | 40.0% |
| ATGGTTGTTCTTAACAGCGG+GGG | + | chr1_1:18521810-18521829 | Msa0012310:intron | 45.0% | |
| TGACTATAAGGAACCAGAGC+CGG | + | chr1_1:18521938-18521957 | Msa0012310:CDS | 45.0% | |
| ! | ACTCTTAGACATGGAGGTAC+CGG | - | chr1_1:18521960-18521979 | None:intergenic | 45.0% |
| GAAGCAAATCACACTGACCT+TGG | + | chr1_1:18522165-18522184 | Msa0012310:CDS | 45.0% | |
| ! | ATGAAGAGGTACATAGGGCA+AGG | + | chr1_1:18522253-18522272 | Msa0012310:CDS | 45.0% |
| ! | TGAAGAGGTACATAGGGCAA+GGG | + | chr1_1:18522254-18522273 | Msa0012310:CDS | 45.0% |
| ATGGTTGTTCTTAACAGCGG+GGG | + | chr1_1:18521810-18521829 | Msa0012310:intron | 45.0% | |
| TGACTATAAGGAACCAGAGC+CGG | + | chr1_1:18521938-18521957 | Msa0012310:CDS | 45.0% | |
| ! | ACTCTTAGACATGGAGGTAC+CGG | - | chr1_1:18521960-18521979 | None:intergenic | 45.0% |
| GAAGCAAATCACACTGACCT+TGG | + | chr1_1:18522165-18522184 | Msa0012310:CDS | 45.0% | |
| ! | ATGAAGAGGTACATAGGGCA+AGG | + | chr1_1:18522253-18522272 | Msa0012310:CDS | 45.0% |
| ! | TGAAGAGGTACATAGGGCAA+GGG | + | chr1_1:18522254-18522273 | Msa0012310:CDS | 45.0% |
| TGCAGTAGGTGTGCAATTGC+AGG | + | chr1_1:18521624-18521643 | Msa0012310:five_prime_UTR | 50.0% | |
| TGCAGTAGGTGTGCAATTGC+AGG | + | chr1_1:18521624-18521643 | Msa0012310:five_prime_UTR | 50.0% | |
| ! | AGAGGTACATAGGGCAAGGG+TGG | + | chr1_1:18522257-18522276 | Msa0012310:CDS | 55.0% |
| ! | AGAGGTACATAGGGCAAGGG+TGG | + | chr1_1:18522257-18522276 | Msa0012310:CDS | 55.0% |
| !! | AGACATGGAGGTACCGGCTC+TGG | - | chr1_1:18521954-18521973 | None:intergenic | 60.0% |
| !! | AGACATGGAGGTACCGGCTC+TGG | - | chr1_1:18521954-18521973 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 18521613 | 18522390 | 18521613 | ID=Msa0012310;Name=Msa0012310 |
| chr1_1 | mRNA | 18521613 | 18522390 | 18521613 | ID=Msa0012310-mRNA-1;Parent=Msa0012310;Name=Msa0012310-mRNA-1;_AED=0.05;_eAED=0.05;_QI=92|1|0.66|1|1|1|3|0|166 |
| chr1_1 | exon | 18521613 | 18521721 | 18521613 | ID=Msa0012310-mRNA-1:exon:6778;Parent=Msa0012310-mRNA-1 |
| chr1_1 | exon | 18521827 | 18522040 | 18521827 | ID=Msa0012310-mRNA-1:exon:6779;Parent=Msa0012310-mRNA-1 |
| chr1_1 | exon | 18522121 | 18522390 | 18522121 | ID=Msa0012310-mRNA-1:exon:6780;Parent=Msa0012310-mRNA-1 |
| chr1_1 | five_prime_UTR | 18521613 | 18521704 | 18521613 | ID=Msa0012310-mRNA-1:five_prime_utr;Parent=Msa0012310-mRNA-1 |
| chr1_1 | CDS | 18521705 | 18521721 | 18521705 | ID=Msa0012310-mRNA-1:cds;Parent=Msa0012310-mRNA-1 |
| chr1_1 | CDS | 18521827 | 18522040 | 18521827 | ID=Msa0012310-mRNA-1:cds;Parent=Msa0012310-mRNA-1 |
| chr1_1 | CDS | 18522121 | 18522390 | 18522121 | ID=Msa0012310-mRNA-1:cds;Parent=Msa0012310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
