AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0015870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0015870 MtrunA17_Chr1g0167501 88.350 103 12 0 60 162 188 290 5.25e-52 167
Msa0015870 MtrunA17_Chr1g0187721 47.154 123 50 5 46 162 29 142 9.25e-27 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0015870 AT4G14380.1 61.111 54 21 0 109 162 147 200 5.84e-18 77.4

Find 35 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATTTCGTGAAGATATA+AGG 0.252185 1_1:+26010360 Msa0015870:CDS
AGGATCACTGTGCGATCAAC+AGG 0.420209 1_1:-26010098 None:intergenic
CGCCACCATTGGCGTTATGC+CGG 0.472214 1_1:-26009571 None:intergenic
AACGGTGGCGGAAGATGCTC+CGG 0.475729 1_1:+26009552 Msa0015870:CDS
CATGGAAAGTTGTGGTTGCT+AGG 0.488094 1_1:+26010294 Msa0015870:CDS
AATGTTGCAAGTGGATCAAA+AGG 0.493765 1_1:+26010322 Msa0015870:CDS
TAGTATAGACCTTGCACGTA+AGG 0.495771 1_1:-26010118 None:intergenic
ACTACTATTCATAGAAGAAA+CGG 0.501679 1_1:-26010177 None:intergenic
CGAAGACTCATCGCCACCAT+TGG 0.503703 1_1:-26009582 None:intergenic
ATACAAGTGATGAGGCCGTT+GGG 0.526601 1_1:+26010044 Msa0015870:CDS
TGGATGAGAGGCAACGGTGG+CGG 0.527006 1_1:+26009540 Msa0015870:CDS
CACAGTGATCCTTACGTGCA+AGG 0.549471 1_1:+26010109 Msa0015870:CDS
CAATGATGTAGACACTATAG+AGG 0.549886 1_1:+26010268 Msa0015870:CDS
CAAGGTCTATACTAATTCAA+AGG 0.568530 1_1:+26010127 Msa0015870:CDS
AACAAGTAGAGAAATTGAAA+AGG 0.571929 1_1:+26010226 Msa0015870:CDS
GTGTAACGAAAGGTTCCTGC+AGG 0.572855 1_1:+26009607 Msa0015870:CDS
ACTGTGCGATCAACAGGTGC+AGG 0.574400 1_1:-26010092 None:intergenic
TAGACACTATAGAGGATGCA+TGG 0.587531 1_1:+26010276 Msa0015870:CDS
TATACAAGTGATGAGGCCGT+TGG 0.587826 1_1:+26010043 Msa0015870:CDS
ATGCTCCGGCATAACGCCAA+TGG 0.590900 1_1:+26009566 Msa0015870:CDS
ATGGTGGCGATGAGTCTTCG+CGG 0.591683 1_1:+26009585 Msa0015870:CDS
CTCCGGCATAACGCCAATGG+TGG 0.596829 1_1:+26009569 Msa0015870:CDS
CATTGCTTGAATTTCATGAG+AGG 0.602579 1_1:+26010399 Msa0015870:CDS
AGTCTTCGCGGTGTAACGAA+AGG 0.615628 1_1:+26009597 Msa0015870:CDS
GTCCGGTGATAATGGATGAG+AGG 0.628523 1_1:+26009528 None:intergenic
TGATAATGGATGAGAGGCAA+CGG 0.629529 1_1:+26009534 None:intergenic
TTGGGACGTTCTAACCTAGT+CGG 0.631727 1_1:+26010062 Msa0015870:CDS
GTGCAGGTTGATAACCGACT+AGG 0.654833 1_1:-26010076 None:intergenic
TAGGTCTCCAATGTTGCAAG+TGG 0.662107 1_1:+26010313 Msa0015870:CDS
AGTAGACTATACAAGTGATG+AGG 0.669120 1_1:+26010036 Msa0015870:intron
AGAGGATGCATGGAAAGTTG+TGG 0.674113 1_1:+26010286 Msa0015870:CDS
AGTAATATTAGTCATGCACA+AGG 0.689144 1_1:+26010200 Msa0015870:CDS
ACTAGGTTAGAACGTCCCAA+CGG 0.697597 1_1:-26010059 None:intergenic
TAATGGATGAGAGGCAACGG+TGG 0.710621 1_1:+26009537 None:intergenic
CTATTCATAGAAGAAACGGA+AGG 0.723878 1_1:-26010173 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAAATAAAAGAGTTCA+GGG + chr1_1:26009866-26009885 Msa0015870:intron 15.0%
!! ATATAAAATAAAAGAGTTCA+GGG + chr1_1:26009866-26009885 Msa0015870:intron 15.0%
!! GATATAAAATAAAAGAGTTC+AGG + chr1_1:26009865-26009884 Msa0015870:intron 20.0%
!! AATATCCAACAAAATAAGTA+GGG - chr1_1:26009984-26010003 None:intergenic 20.0%
!! TAATATCCAACAAAATAAGT+AGG - chr1_1:26009985-26010004 None:intergenic 20.0%
!! ATAAATTTCGTGAAGATATA+AGG + chr1_1:26010360-26010379 Msa0015870:CDS 20.0%
!! GATATAAAATAAAAGAGTTC+AGG + chr1_1:26009865-26009884 Msa0015870:intron 20.0%
!! AATATCCAACAAAATAAGTA+GGG - chr1_1:26009984-26010003 None:intergenic 20.0%
!! TAATATCCAACAAAATAAGT+AGG - chr1_1:26009985-26010004 None:intergenic 20.0%
!! ATAAATTTCGTGAAGATATA+AGG + chr1_1:26010360-26010379 Msa0015870:CDS 20.0%
! ATATCCAACAAAATAAGTAG+GGG - chr1_1:26009983-26010002 None:intergenic 25.0%
! ACTACTATTCATAGAAGAAA+CGG - chr1_1:26010180-26010199 None:intergenic 25.0%
! AACAAGTAGAGAAATTGAAA+AGG + chr1_1:26010226-26010245 Msa0015870:CDS 25.0%
! ATATCCAACAAAATAAGTAG+GGG - chr1_1:26009983-26010002 None:intergenic 25.0%
! ACTACTATTCATAGAAGAAA+CGG - chr1_1:26010180-26010199 None:intergenic 25.0%
! AACAAGTAGAGAAATTGAAA+AGG + chr1_1:26010226-26010245 Msa0015870:CDS 25.0%
! ATAATCAACTCCGGATTTTA+CGG + chr1_1:26009755-26009774 Msa0015870:intron 30.0%
TATCCAACAAAATAAGTAGG+GGG - chr1_1:26009982-26010001 None:intergenic 30.0%
CAAGGTCTATACTAATTCAA+AGG + chr1_1:26010127-26010146 Msa0015870:CDS 30.0%
AGTAATATTAGTCATGCACA+AGG + chr1_1:26010200-26010219 Msa0015870:CDS 30.0%
! ATAATCAACTCCGGATTTTA+CGG + chr1_1:26009755-26009774 Msa0015870:intron 30.0%
TATCCAACAAAATAAGTAGG+GGG - chr1_1:26009982-26010001 None:intergenic 30.0%
CAAGGTCTATACTAATTCAA+AGG + chr1_1:26010127-26010146 Msa0015870:CDS 30.0%
AGTAATATTAGTCATGCACA+AGG + chr1_1:26010200-26010219 Msa0015870:CDS 30.0%
AAAAGTGAACCATACCTTGA+GGG + chr1_1:26009695-26009714 Msa0015870:intron 35.0%
TTGCAACAGAGTAAATTACC+TGG + chr1_1:26009913-26009932 Msa0015870:intron 35.0%
! TACTTTTCTGCAATCAAACC+AGG - chr1_1:26009934-26009953 None:intergenic 35.0%
ATCCAACAAAATAAGTAGGG+GGG - chr1_1:26009981-26010000 None:intergenic 35.0%
AGTAGACTATACAAGTGATG+AGG + chr1_1:26010036-26010055 Msa0015870:intron 35.0%
CTATTCATAGAAGAAACGGA+AGG - chr1_1:26010176-26010195 None:intergenic 35.0%
CAATGATGTAGACACTATAG+AGG + chr1_1:26010268-26010287 Msa0015870:CDS 35.0%
! TTTTGATCCACTTGCAACAT+TGG - chr1_1:26010323-26010342 None:intergenic 35.0%
AATGTTGCAAGTGGATCAAA+AGG + chr1_1:26010322-26010341 Msa0015870:CDS 35.0%
CATTGCTTGAATTTCATGAG+AGG + chr1_1:26010399-26010418 Msa0015870:CDS 35.0%
AAAAGTGAACCATACCTTGA+GGG + chr1_1:26009695-26009714 Msa0015870:intron 35.0%
TTGCAACAGAGTAAATTACC+TGG + chr1_1:26009913-26009932 Msa0015870:intron 35.0%
! TACTTTTCTGCAATCAAACC+AGG - chr1_1:26009934-26009953 None:intergenic 35.0%
ATCCAACAAAATAAGTAGGG+GGG - chr1_1:26009981-26010000 None:intergenic 35.0%
AGTAGACTATACAAGTGATG+AGG + chr1_1:26010036-26010055 Msa0015870:intron 35.0%
CTATTCATAGAAGAAACGGA+AGG - chr1_1:26010176-26010195 None:intergenic 35.0%
CAATGATGTAGACACTATAG+AGG + chr1_1:26010268-26010287 Msa0015870:CDS 35.0%
! TTTTGATCCACTTGCAACAT+TGG - chr1_1:26010323-26010342 None:intergenic 35.0%
AATGTTGCAAGTGGATCAAA+AGG + chr1_1:26010322-26010341 Msa0015870:CDS 35.0%
CATTGCTTGAATTTCATGAG+AGG + chr1_1:26010399-26010418 Msa0015870:CDS 35.0%
GAAAAGTGAACCATACCTTG+AGG + chr1_1:26009694-26009713 Msa0015870:intron 40.0%
AAGCAATGCCACCCTTATAT+AGG + chr1_1:26009720-26009739 Msa0015870:intron 40.0%
GCATTGGGAATAATCAACTC+CGG + chr1_1:26009746-26009765 Msa0015870:intron 40.0%
TAATCACACGCCGTAAAATC+CGG - chr1_1:26009768-26009787 None:intergenic 40.0%
! GATTTTACGGCGTGTGATTA+GGG + chr1_1:26009768-26009787 Msa0015870:intron 40.0%
ACTGTTAGAGGACACATGTA+GGG + chr1_1:26009791-26009810 Msa0015870:intron 40.0%
GATTGCAGAAAAGTAAGCAG+CGG + chr1_1:26009939-26009958 Msa0015870:intron 40.0%
!!! ATCCCCCCTACTTATTTTGT+TGG + chr1_1:26009976-26009995 Msa0015870:intron 40.0%
TAGTATAGACCTTGCACGTA+AGG - chr1_1:26010121-26010140 None:intergenic 40.0%
TAGACACTATAGAGGATGCA+TGG + chr1_1:26010276-26010295 Msa0015870:CDS 40.0%
GAAAAGTGAACCATACCTTG+AGG + chr1_1:26009694-26009713 Msa0015870:intron 40.0%
AAGCAATGCCACCCTTATAT+AGG + chr1_1:26009720-26009739 Msa0015870:intron 40.0%
GCATTGGGAATAATCAACTC+CGG + chr1_1:26009746-26009765 Msa0015870:intron 40.0%
TAATCACACGCCGTAAAATC+CGG - chr1_1:26009768-26009787 None:intergenic 40.0%
! GATTTTACGGCGTGTGATTA+GGG + chr1_1:26009768-26009787 Msa0015870:intron 40.0%
ACTGTTAGAGGACACATGTA+GGG + chr1_1:26009791-26009810 Msa0015870:intron 40.0%
GATTGCAGAAAAGTAAGCAG+CGG + chr1_1:26009939-26009958 Msa0015870:intron 40.0%
!!! ATCCCCCCTACTTATTTTGT+TGG + chr1_1:26009976-26009995 Msa0015870:intron 40.0%
TAGTATAGACCTTGCACGTA+AGG - chr1_1:26010121-26010140 None:intergenic 40.0%
TAGACACTATAGAGGATGCA+TGG + chr1_1:26010276-26010295 Msa0015870:CDS 40.0%
TCCAACGCTCAAGTCATTGA+TGG + chr1_1:26009641-26009660 Msa0015870:intron 45.0%
TCCATCAATGACTTGAGCGT+TGG - chr1_1:26009645-26009664 None:intergenic 45.0%
ATTGCTTCACCCTCAAGGTA+TGG - chr1_1:26009707-26009726 None:intergenic 45.0%
! GGATTTTACGGCGTGTGATT+AGG + chr1_1:26009767-26009786 Msa0015870:intron 45.0%
GTGTGATTAGGGACTGTTAG+AGG + chr1_1:26009779-26009798 Msa0015870:intron 45.0%
GACTGTTAGAGGACACATGT+AGG + chr1_1:26009790-26009809 Msa0015870:intron 45.0%
CAGAAAAGTAAGCAGCGGTT+AGG + chr1_1:26009944-26009963 Msa0015870:intron 45.0%
TATACAAGTGATGAGGCCGT+TGG + chr1_1:26010043-26010062 Msa0015870:CDS 45.0%
ATACAAGTGATGAGGCCGTT+GGG + chr1_1:26010044-26010063 Msa0015870:CDS 45.0%
ACTAGGTTAGAACGTCCCAA+CGG - chr1_1:26010062-26010081 None:intergenic 45.0%
TTGGGACGTTCTAACCTAGT+CGG + chr1_1:26010062-26010081 Msa0015870:CDS 45.0%
AGAGGATGCATGGAAAGTTG+TGG + chr1_1:26010286-26010305 Msa0015870:CDS 45.0%
CATGGAAAGTTGTGGTTGCT+AGG + chr1_1:26010294-26010313 Msa0015870:CDS 45.0%
TAGGTCTCCAATGTTGCAAG+TGG + chr1_1:26010313-26010332 Msa0015870:CDS 45.0%
TCCAACGCTCAAGTCATTGA+TGG + chr1_1:26009641-26009660 Msa0015870:intron 45.0%
TCCATCAATGACTTGAGCGT+TGG - chr1_1:26009645-26009664 None:intergenic 45.0%
ATTGCTTCACCCTCAAGGTA+TGG - chr1_1:26009707-26009726 None:intergenic 45.0%
! GGATTTTACGGCGTGTGATT+AGG + chr1_1:26009767-26009786 Msa0015870:intron 45.0%
GTGTGATTAGGGACTGTTAG+AGG + chr1_1:26009779-26009798 Msa0015870:intron 45.0%
GACTGTTAGAGGACACATGT+AGG + chr1_1:26009790-26009809 Msa0015870:intron 45.0%
CAGAAAAGTAAGCAGCGGTT+AGG + chr1_1:26009944-26009963 Msa0015870:intron 45.0%
TATACAAGTGATGAGGCCGT+TGG + chr1_1:26010043-26010062 Msa0015870:CDS 45.0%
ATACAAGTGATGAGGCCGTT+GGG + chr1_1:26010044-26010063 Msa0015870:CDS 45.0%
ACTAGGTTAGAACGTCCCAA+CGG - chr1_1:26010062-26010081 None:intergenic 45.0%
TTGGGACGTTCTAACCTAGT+CGG + chr1_1:26010062-26010081 Msa0015870:CDS 45.0%
AGAGGATGCATGGAAAGTTG+TGG + chr1_1:26010286-26010305 Msa0015870:CDS 45.0%
CATGGAAAGTTGTGGTTGCT+AGG + chr1_1:26010294-26010313 Msa0015870:CDS 45.0%
TAGGTCTCCAATGTTGCAAG+TGG + chr1_1:26010313-26010332 Msa0015870:CDS 45.0%
AGTCTTCGCGGTGTAACGAA+AGG + chr1_1:26009597-26009616 Msa0015870:CDS 50.0%
GTGTAACGAAAGGTTCCTGC+AGG + chr1_1:26009607-26009626 Msa0015870:CDS 50.0%
AATGCGCGCCTATATAAGGG+TGG - chr1_1:26009731-26009750 None:intergenic 50.0%
ACCCTTATATAGGCGCGCAT+TGG + chr1_1:26009730-26009749 Msa0015870:intron 50.0%
CCCAATGCGCGCCTATATAA+GGG - chr1_1:26009734-26009753 None:intergenic 50.0%
CCCTTATATAGGCGCGCATT+GGG + chr1_1:26009731-26009750 Msa0015870:intron 50.0%
TCCCAATGCGCGCCTATATA+AGG - chr1_1:26009735-26009754 None:intergenic 50.0%
!! GTGCAGGTTGATAACCGACT+AGG - chr1_1:26010079-26010098 None:intergenic 50.0%
AGGATCACTGTGCGATCAAC+AGG - chr1_1:26010101-26010120 None:intergenic 50.0%
CACAGTGATCCTTACGTGCA+AGG + chr1_1:26010109-26010128 Msa0015870:CDS 50.0%
AGTCTTCGCGGTGTAACGAA+AGG + chr1_1:26009597-26009616 Msa0015870:CDS 50.0%
GTGTAACGAAAGGTTCCTGC+AGG + chr1_1:26009607-26009626 Msa0015870:CDS 50.0%
AATGCGCGCCTATATAAGGG+TGG - chr1_1:26009731-26009750 None:intergenic 50.0%
ACCCTTATATAGGCGCGCAT+TGG + chr1_1:26009730-26009749 Msa0015870:intron 50.0%
CCCAATGCGCGCCTATATAA+GGG - chr1_1:26009734-26009753 None:intergenic 50.0%
CCCTTATATAGGCGCGCATT+GGG + chr1_1:26009731-26009750 Msa0015870:intron 50.0%
TCCCAATGCGCGCCTATATA+AGG - chr1_1:26009735-26009754 None:intergenic 50.0%
!! GTGCAGGTTGATAACCGACT+AGG - chr1_1:26010079-26010098 None:intergenic 50.0%
AGGATCACTGTGCGATCAAC+AGG - chr1_1:26010101-26010120 None:intergenic 50.0%
CACAGTGATCCTTACGTGCA+AGG + chr1_1:26010109-26010128 Msa0015870:CDS 50.0%
ATGCTCCGGCATAACGCCAA+TGG + chr1_1:26009566-26009585 Msa0015870:CDS 55.0%
CGAAGACTCATCGCCACCAT+TGG - chr1_1:26009585-26009604 None:intergenic 55.0%
! ATGGTGGCGATGAGTCTTCG+CGG + chr1_1:26009585-26009604 Msa0015870:CDS 55.0%
GTGGCATTGCTTCACCCTCA+AGG - chr1_1:26009712-26009731 None:intergenic 55.0%
!! CGTGGAGGTGTTCTCGTATG+TGG + chr1_1:26009823-26009842 Msa0015870:intron 55.0%
ACTGTGCGATCAACAGGTGC+AGG - chr1_1:26010095-26010114 None:intergenic 55.0%
ATGCTCCGGCATAACGCCAA+TGG + chr1_1:26009566-26009585 Msa0015870:CDS 55.0%
CGAAGACTCATCGCCACCAT+TGG - chr1_1:26009585-26009604 None:intergenic 55.0%
! ATGGTGGCGATGAGTCTTCG+CGG + chr1_1:26009585-26009604 Msa0015870:CDS 55.0%
GTGGCATTGCTTCACCCTCA+AGG - chr1_1:26009712-26009731 None:intergenic 55.0%
!! CGTGGAGGTGTTCTCGTATG+TGG + chr1_1:26009823-26009842 Msa0015870:intron 55.0%
ACTGTGCGATCAACAGGTGC+AGG - chr1_1:26010095-26010114 None:intergenic 55.0%
TGGATGAGAGGCAACGGTGG+CGG + chr1_1:26009540-26009559 Msa0015870:CDS 60.0%
!! AACGGTGGCGGAAGATGCTC+CGG + chr1_1:26009552-26009571 Msa0015870:CDS 60.0%
CTCCGGCATAACGCCAATGG+TGG + chr1_1:26009569-26009588 Msa0015870:CDS 60.0%
CGCCACCATTGGCGTTATGC+CGG - chr1_1:26009574-26009593 None:intergenic 60.0%
! TGGAGTGCTAACGTGCCTGC+AGG - chr1_1:26009625-26009644 None:intergenic 60.0%
CATGTAGGGTGATCCTCGCG+TGG + chr1_1:26009805-26009824 Msa0015870:intron 60.0%
TACGAGAACACCTCCACGCG+AGG - chr1_1:26009821-26009840 None:intergenic 60.0%
TGGATGAGAGGCAACGGTGG+CGG + chr1_1:26009540-26009559 Msa0015870:CDS 60.0%
!! AACGGTGGCGGAAGATGCTC+CGG + chr1_1:26009552-26009571 Msa0015870:CDS 60.0%
CTCCGGCATAACGCCAATGG+TGG + chr1_1:26009569-26009588 Msa0015870:CDS 60.0%
CGCCACCATTGGCGTTATGC+CGG - chr1_1:26009574-26009593 None:intergenic 60.0%
! TGGAGTGCTAACGTGCCTGC+AGG - chr1_1:26009625-26009644 None:intergenic 60.0%
CATGTAGGGTGATCCTCGCG+TGG + chr1_1:26009805-26009824 Msa0015870:intron 60.0%
TACGAGAACACCTCCACGCG+AGG - chr1_1:26009821-26009840 None:intergenic 60.0%
GTAGGGTGATCCTCGCGTGG+AGG + chr1_1:26009808-26009827 Msa0015870:intron 65.0%
GTAGGGTGATCCTCGCGTGG+AGG + chr1_1:26009808-26009827 Msa0015870:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 26009539 26010439 26009539 ID=Msa0015870;Name=Msa0015870
chr1_1 mRNA 26009539 26010439 26009539 ID=Msa0015870-mRNA-1;Parent=Msa0015870;Name=Msa0015870-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|0.5|1|1|2|0|162
chr1_1 exon 26009539 26009628 26009539 ID=Msa0015870-mRNA-1:exon:8888;Parent=Msa0015870-mRNA-1
chr1_1 exon 26010041 26010439 26010041 ID=Msa0015870-mRNA-1:exon:8889;Parent=Msa0015870-mRNA-1
chr1_1 CDS 26009539 26009628 26009539 ID=Msa0015870-mRNA-1:cds;Parent=Msa0015870-mRNA-1
chr1_1 CDS 26010041 26010439 26010041 ID=Msa0015870-mRNA-1:cds;Parent=Msa0015870-mRNA-1
Gene Sequence

>Msa0015870

ATGGATGAGAGGCAACGGTGGCGGAAGATGCTCCGGCATAACGCCAATGGTGGCGATGAGTCTTCGCGGTGTAACGAAAGGTTCCTGCAGACTATACAAGTGATGAGGCCGTTGGGACGTTCTAACCTAGTCGGTTATCAACCTGCACCTGTTGATCGCACAGTGATCCTTACGTGCAAGGTCTATACTAATTCAAAGGTAAATGATCTCTATTCTCAAACTCCTTCCGTTTCTTCTATGAATAGTAGTAGTAATATTAGTCATGCACAAGGTGAAACAAGTAGAGAAATTGAAAAGGTACATGTTGATGACAATAACAATGATGTAGACACTATAGAGGATGCATGGAAAGTTGTGGTTGCTAGGTCTCCAATGTTGCAAGTGGATCAAAAGGCTGAAGAATTTATCTATAAATTTCGTGAAGATATAAGGCTTCAGAAAGAGAAATCATTGCTTGAATTTCATGAGAGGCTAGCAAGAAGTACCTAA

Protein sequence

>Msa0015870

MDERQRWRKMLRHNANGGDESSRCNERFLQTIQVMRPLGRSNLVGYQPAPVDRTVILTCKVYTNSKVNDLYSQTPSVSSMNSSSNISHAQGETSREIEKVHVDDNNNDVDTIEDAWKVVVARSPMLQVDQKAEEFIYKFREDIRLQKEKSLLEFHERLARST*