AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0028790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028790 MtrunA17_Chr1g0185321 86.243 189 24 2 1 188 1 188 4.06e-92 266
Msa0028790 MtrunA17_Chr5g0395001 52.514 179 68 2 1 179 1 162 4.98e-49 156
Msa0028790 MtrunA17_Chr1g0205201 45.402 174 58 1 10 183 10 146 3.33e-46 149
Msa0028790 MtrunA17_Chr1g0205181 42.553 141 76 2 1 139 1 138 2.55e-29 106
Msa0028790 MtrunA17_Chr7g0260061 44.604 139 63 4 6 137 8 139 1.35e-27 102
Msa0028790 MtrunA17_Chr1g0205191 59.036 83 32 1 24 106 24 104 7.89e-27 98.6
Msa0028790 MtrunA17_Chr3g0118471 40.860 93 52 2 24 113 46 138 1.97e-15 71.2
Msa0028790 MtrunA17_Chr8g0356191 29.630 189 116 7 1 184 9 185 8.38e-15 69.3
Msa0028790 MtrunA17_Chr5g0394991 34.659 176 92 9 6 161 8 180 1.54e-14 68.9
Msa0028790 MtrunA17_Chr8g0378461 27.976 168 113 4 22 188 30 190 1.29e-12 63.5
Msa0028790 MtrunA17_Chr5g0429901 32.283 127 68 4 28 151 35 146 2.35e-12 62.8
Msa0028790 MtrunA17_Chr6g0484651 34.653 101 60 2 3 98 13 112 7.25e-12 60.1
Msa0028790 MtrunA17_Chr1g0201291 49.254 67 31 2 10 75 10 74 1.13e-11 58.5
Msa0028790 MtrunA17_Chr7g0219391 34.314 102 63 3 6 105 13 112 1.62e-11 59.3
Msa0028790 MtrunA17_Chr2g0278981 29.839 124 78 3 6 121 7 129 1.62e-11 60.5
Msa0028790 MtrunA17_Chr3g0121381 33.333 84 54 2 23 105 26 108 2.52e-11 59.7
Msa0028790 MtrunA17_Chr2g0278971 33.028 109 67 3 7 110 52 159 3.35e-11 60.1
Msa0028790 MtrunA17_Chr7g0260071 38.462 91 47 3 35 117 41 130 9.70e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028790 AT3G22600.1 45.745 188 78 5 1 185 3 169 3.28e-40 134
Msa0028790 AT1G03103.1 46.369 179 83 5 7 181 2 171 3.48e-39 132
Msa0028790 AT2G48130.2 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT2G48130.6 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT2G48130.4 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT2G48130.5 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT2G48130.1 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT2G48130.3 41.989 181 94 4 4 181 5 177 1.25e-37 128
Msa0028790 AT4G14815.1 40.909 176 67 6 8 182 5 144 1.89e-31 112
Msa0028790 AT2G13820.3 32.402 179 102 6 8 183 18 180 1.48e-17 76.6
Msa0028790 AT2G13820.1 32.402 179 102 6 8 183 6 168 1.72e-17 76.3
Msa0028790 AT3G43720.2 32.335 167 88 5 28 184 38 189 3.47e-16 73.2
Msa0028790 AT3G43720.1 31.361 169 89 5 28 184 38 191 1.03e-15 72.0
Msa0028790 AT1G55260.1 27.160 162 112 4 23 183 71 227 1.06e-14 70.1
Msa0028790 AT2G13820.2 37.374 99 58 3 10 105 8 105 1.62e-14 67.4
Msa0028790 AT1G55260.2 27.160 162 109 4 23 183 71 224 2.50e-14 68.9
Msa0028790 AT2G44290.1 26.866 201 122 7 1 184 9 201 6.56e-14 67.4
Msa0028790 AT5G09370.3 35.714 112 67 3 23 132 23 131 1.44e-13 65.5
Msa0028790 AT2G44300.1 30.000 180 103 9 21 184 28 200 3.11e-13 65.9
Msa0028790 AT4G08670.1 35.465 172 81 9 26 186 46 198 7.72e-13 64.7
Msa0028790 AT5G09370.2 38.372 86 49 2 23 106 23 106 1.81e-12 62.0
Msa0028790 AT5G09370.1 38.372 86 49 2 23 106 23 106 2.04e-12 62.4
Msa0028790 AT1G05450.2 34.884 172 96 7 30 188 36 204 2.54e-12 63.2
Msa0028790 AT5G64080.1 30.380 158 86 5 28 183 43 178 1.32e-11 60.8
Msa0028790 AT5G64080.2 31.056 161 77 7 28 183 43 174 1.92e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTAGTTCCAAGATAAA+AGG 0.236232 1_1:+53844738 Msa0028790:five_prime_UTR
AAAGGAGTAATGGCATTTAA+AGG 0.261095 1_1:+53844756 Msa0028790:exon
AATGGTGGTGGTTCAACTTT+TGG 0.266499 1_1:+53844981 Msa0028790:CDS
GTCTCTCCCTTCTGCTTGTA+AGG 0.299840 1_1:+53845031 Msa0028790:CDS
TGTCTATGCTCCCTCCTTAA+TGG 0.316118 1_1:+53844963 Msa0028790:CDS
TCAAGTTCAACTTCTTCAAT+TGG 0.356147 1_1:+53845226 Msa0028790:CDS
CCCTTGTCTAAACTACATAA+TGG 0.358396 1_1:+53844872 Msa0028790:CDS
AAGTTGAACCACCACCATTA+AGG 0.366872 1_1:-53844977 None:intergenic
GAAGGTGTTATTGTGCCTTC+AGG 0.369459 1_1:-53845277 None:intergenic
GACGAGCCAGCGTCTGTTGA+TGG 0.377260 1_1:-53845472 None:intergenic
TTACCTTTGCACTGGCTAAC+CGG 0.388855 1_1:-53845065 None:intergenic
ATTGAAGAAGTTGAACTTGA+AGG 0.407603 1_1:-53845223 None:intergenic
AAGGAGTAATGGCATTTAAA+GGG 0.456484 1_1:+53844757 Msa0028790:exon
TCAGACTTTCCCTCATCCTC+AGG 0.458230 1_1:+53845304 Msa0028790:CDS
TGCTCCCTCCTTAATGGTGG+TGG 0.462126 1_1:+53844969 Msa0028790:CDS
TCTTCAATTGGTTCACCTGT+TGG 0.462385 1_1:+53845238 Msa0028790:CDS
CCTTGTCTAAACTACATAAT+GGG 0.473365 1_1:+53844873 Msa0028790:CDS
GATTCAGTTGGAGCACCAAC+AGG 0.475748 1_1:-53845253 None:intergenic
ACTCAAAATGAAGCTCAATC+AGG 0.480107 1_1:+53844822 Msa0028790:CDS
GTGCCTTCAGGTGATTCAGT+TGG 0.485931 1_1:-53845265 None:intergenic
ATAGACACTGTGGAGAAGAT+TGG 0.486063 1_1:-53844947 None:intergenic
ACAGACGCTGGCTCGTCTGA+TGG 0.500132 1_1:+53845478 Msa0028790:CDS
AGTATATATACCTGAGGATG+AGG 0.503241 1_1:-53845314 None:intergenic
ATTCAAACTTACCTTTGCAC+TGG 0.504688 1_1:-53845073 None:intergenic
TTCCAAGATAAAAGGAGTAA+TGG 0.511922 1_1:+53844746 Msa0028790:exon
GTATATCCTATGTCTAGTTA+TGG 0.517596 1_1:+53844779 Msa0028790:CDS
CCCATTATGTAGTTTAGACA+AGG 0.536489 1_1:-53844873 None:intergenic
GTATACATAAAATCAAAACT+TGG 0.540554 1_1:-53845566 None:intergenic
ACGAGCCAGCGTCTGTTGAT+GGG 0.545167 1_1:-53845471 None:intergenic
GTTTGAACCTTACAAGCAGA+AGG 0.551664 1_1:-53845038 None:intergenic
TTGTAAGGTTCAAACTCCAC+CGG 0.580289 1_1:+53845046 Msa0028790:CDS
GTTGAACTTGAAGGAGATGT+TGG 0.580975 1_1:-53845214 None:intergenic
TGTGAGCAACATGAAGATGA+TGG 0.581104 1_1:-53844909 None:intergenic
CTACGACCATAACTAGACAT+AGG 0.586523 1_1:-53844785 None:intergenic
CTATGCTCCCTCCTTAATGG+TGG 0.612240 1_1:+53844966 Msa0028790:CDS
CCTTTGCACTGGCTAACCGG+TGG 0.616670 1_1:-53845062 None:intergenic
TTGAACCACCACCATTAAGG+AGG 0.620999 1_1:-53844974 None:intergenic
GAGGGAAAGTCTGAAGCAGA+AGG 0.627664 1_1:-53845295 None:intergenic
ATGTCTAGTTATGGTCGTAG+TGG 0.633614 1_1:+53844788 Msa0028790:CDS
AGCGTCCCATCAACAGACGC+TGG 0.637645 1_1:+53845466 Msa0028790:CDS
CCACCGGTTAGCCAGTGCAA+AGG 0.638372 1_1:+53845062 Msa0028790:CDS
GTATATATACCTGAGGATGA+GGG 0.643487 1_1:-53845313 None:intergenic
CCATTATGTAGTTTAGACAA+GGG 0.644176 1_1:-53844872 None:intergenic
TTTGAACCTTACAAGCAGAA+GGG 0.651803 1_1:-53845037 None:intergenic
TGAACCACCACCATTAAGGA+GGG 0.659706 1_1:-53844973 None:intergenic
GCTCCAACTGAATCACCTGA+AGG 0.660479 1_1:+53845262 Msa0028790:CDS
CAAGAAGGGAAAGAACAAAG+TGG 0.672272 1_1:-53845522 None:intergenic
AGGAGGGAGCATAGACACTG+TGG 0.710687 1_1:-53844957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTAGTTCCAAGATAAA+AGG + chr1_1:53844738-53844757 Msa0028790:five_prime_UTR 20.0%
!! TATTTACATGGTATGAATTT+AGG + chr1_1:53845181-53845200 Msa0028790:intron 20.0%
!! TCTATTACAAATGATTACTT+GGG - chr1_1:53845373-53845392 None:intergenic 20.0%
!!! TTCATTTTGAGTACACATAT+TGG - chr1_1:53844814-53844833 None:intergenic 25.0%
! ATACCAATGACTTATTTACA+TGG + chr1_1:53845169-53845188 Msa0028790:intron 25.0%
! ATACCATGTAAATAAGTCAT+TGG - chr1_1:53845175-53845194 None:intergenic 25.0%
! AGTATTAGTATATATACCTG+AGG - chr1_1:53845323-53845342 None:intergenic 25.0%
! GTCTATTACAAATGATTACT+TGG - chr1_1:53845374-53845393 None:intergenic 25.0%
!!! TTTATTGATTCACATGGTTT+TGG + chr1_1:53845419-53845438 Msa0028790:intron 25.0%
TTCCAAGATAAAAGGAGTAA+TGG + chr1_1:53844746-53844765 Msa0028790:exon 30.0%
AAAGGAGTAATGGCATTTAA+AGG + chr1_1:53844756-53844775 Msa0028790:exon 30.0%
AAGGAGTAATGGCATTTAAA+GGG + chr1_1:53844757-53844776 Msa0028790:exon 30.0%
GTATATCCTATGTCTAGTTA+TGG + chr1_1:53844779-53844798 Msa0028790:CDS 30.0%
CCATTATGTAGTTTAGACAA+GGG - chr1_1:53844875-53844894 None:intergenic 30.0%
CCTTGTCTAAACTACATAAT+GGG + chr1_1:53844873-53844892 Msa0028790:CDS 30.0%
TTACATGGTATGAATTTAGG+TGG + chr1_1:53845184-53845203 Msa0028790:intron 30.0%
ATTGAAGAAGTTGAACTTGA+AGG - chr1_1:53845226-53845245 None:intergenic 30.0%
TCAAGTTCAACTTCTTCAAT+TGG + chr1_1:53845226-53845245 Msa0028790:CDS 30.0%
!! ATAGCGTTTATTGATTCACA+TGG + chr1_1:53845413-53845432 Msa0028790:intron 30.0%
AAAAAACACAATGGCAAGAA+GGG - chr1_1:53845539-53845558 None:intergenic 30.0%
! TGCCATTACTCCTTTTATCT+TGG - chr1_1:53844751-53844770 None:intergenic 35.0%
ACTCAAAATGAAGCTCAATC+AGG + chr1_1:53844822-53844841 Msa0028790:CDS 35.0%
CCCTTGTCTAAACTACATAA+TGG + chr1_1:53844872-53844891 Msa0028790:CDS 35.0%
CCCATTATGTAGTTTAGACA+AGG - chr1_1:53844876-53844895 None:intergenic 35.0%
TTTGAACCTTACAAGCAGAA+GGG - chr1_1:53845040-53845059 None:intergenic 35.0%
ATTCAAACTTACCTTTGCAC+TGG - chr1_1:53845076-53845095 None:intergenic 35.0%
GTATATATACCTGAGGATGA+GGG - chr1_1:53845316-53845335 None:intergenic 35.0%
AGTATATATACCTGAGGATG+AGG - chr1_1:53845317-53845336 None:intergenic 35.0%
CAAAAAACACAATGGCAAGA+AGG - chr1_1:53845540-53845559 None:intergenic 35.0%
GGTGACAACAAAAAACACAA+TGG - chr1_1:53845548-53845567 None:intergenic 35.0%
CTACGACCATAACTAGACAT+AGG - chr1_1:53844788-53844807 None:intergenic 40.0%
ATGTCTAGTTATGGTCGTAG+TGG + chr1_1:53844788-53844807 Msa0028790:CDS 40.0%
TGTGAGCAACATGAAGATGA+TGG - chr1_1:53844912-53844931 None:intergenic 40.0%
ATAGACACTGTGGAGAAGAT+TGG - chr1_1:53844950-53844969 None:intergenic 40.0%
AAGTTGAACCACCACCATTA+AGG - chr1_1:53844980-53844999 None:intergenic 40.0%
!!! AATGGTGGTGGTTCAACTTT+TGG + chr1_1:53844981-53845000 Msa0028790:CDS 40.0%
GTTTGAACCTTACAAGCAGA+AGG - chr1_1:53845041-53845060 None:intergenic 40.0%
TTGTAAGGTTCAAACTCCAC+CGG + chr1_1:53845046-53845065 Msa0028790:CDS 40.0%
CCAATTATTACAGACGCTTC+AGG - chr1_1:53845136-53845155 None:intergenic 40.0%
! CCTGAAGCGTCTGTAATAAT+TGG + chr1_1:53845133-53845152 Msa0028790:intron 40.0%
GTTGAACTTGAAGGAGATGT+TGG - chr1_1:53845217-53845236 None:intergenic 40.0%
! TCTTCAATTGGTTCACCTGT+TGG + chr1_1:53845238-53845257 Msa0028790:CDS 40.0%
!!! ACATGGTTTTGGTGAATTGC+AGG + chr1_1:53845430-53845449 Msa0028790:intron 40.0%
CAAGAAGGGAAAGAACAAAG+TGG - chr1_1:53845525-53845544 None:intergenic 40.0%
TGTCTATGCTCCCTCCTTAA+TGG + chr1_1:53844963-53844982 Msa0028790:CDS 45.0%
TGAACCACCACCATTAAGGA+GGG - chr1_1:53844976-53844995 None:intergenic 45.0%
TTGAACCACCACCATTAAGG+AGG - chr1_1:53844977-53844996 None:intergenic 45.0%
!! TTACCTTTGCACTGGCTAAC+CGG - chr1_1:53845068-53845087 None:intergenic 45.0%
!! TTACAGACGCTTCAGGTTGA+AGG - chr1_1:53845129-53845148 None:intergenic 45.0%
!! GAAGGTGTTATTGTGCCTTC+AGG - chr1_1:53845280-53845299 None:intergenic 45.0%
! TTTTGGTGAATTGCAGGTGC+AGG + chr1_1:53845436-53845455 Msa0028790:intron 45.0%
CTATGCTCCCTCCTTAATGG+TGG + chr1_1:53844966-53844985 Msa0028790:CDS 50.0%
GTCTCTCCCTTCTGCTTGTA+AGG + chr1_1:53845031-53845050 Msa0028790:CDS 50.0%
! GATTCAGTTGGAGCACCAAC+AGG - chr1_1:53845256-53845275 None:intergenic 50.0%
GCTCCAACTGAATCACCTGA+AGG + chr1_1:53845262-53845281 Msa0028790:CDS 50.0%
GTGCCTTCAGGTGATTCAGT+TGG - chr1_1:53845268-53845287 None:intergenic 50.0%
! GAGGGAAAGTCTGAAGCAGA+AGG - chr1_1:53845298-53845317 None:intergenic 50.0%
TCAGACTTTCCCTCATCCTC+AGG + chr1_1:53845304-53845323 Msa0028790:CDS 50.0%
! AGGAGGGAGCATAGACACTG+TGG - chr1_1:53844960-53844979 None:intergenic 55.0%
TGCTCCCTCCTTAATGGTGG+TGG + chr1_1:53844969-53844988 Msa0028790:CDS 55.0%
ACGAGCCAGCGTCTGTTGAT+GGG - chr1_1:53845474-53845493 None:intergenic 55.0%
!! CCTTTGCACTGGCTAACCGG+TGG - chr1_1:53845065-53845084 None:intergenic 60.0%
! CCACCGGTTAGCCAGTGCAA+AGG + chr1_1:53845062-53845081 Msa0028790:CDS 60.0%
AGCGTCCCATCAACAGACGC+TGG + chr1_1:53845466-53845485 Msa0028790:CDS 60.0%
GACGAGCCAGCGTCTGTTGA+TGG - chr1_1:53845475-53845494 None:intergenic 60.0%
!! ACAGACGCTGGCTCGTCTGA+TGG + chr1_1:53845478-53845497 Msa0028790:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 53844715 53845576 53844715 ID=Msa0028790;Name=Msa0028790
chr1_1 mRNA 53844715 53845576 53844715 ID=Msa0028790-mRNA-1;Parent=Msa0028790;Name=Msa0028790-mRNA-1;_AED=0.18;_eAED=0.18;_QI=50|1|0.66|1|1|1|3|0|188
chr1_1 exon 53844715 53845083 53844715 ID=Msa0028790-mRNA-1:exon:15908;Parent=Msa0028790-mRNA-1
chr1_1 exon 53845203 53845325 53845203 ID=Msa0028790-mRNA-1:exon:15909;Parent=Msa0028790-mRNA-1
chr1_1 exon 53845452 53845576 53845452 ID=Msa0028790-mRNA-1:exon:15910;Parent=Msa0028790-mRNA-1
chr1_1 five_prime_UTR 53844715 53844764 53844715 ID=Msa0028790-mRNA-1:five_prime_utr;Parent=Msa0028790-mRNA-1
chr1_1 CDS 53844765 53845083 53844765 ID=Msa0028790-mRNA-1:cds;Parent=Msa0028790-mRNA-1
chr1_1 CDS 53845203 53845325 53845203 ID=Msa0028790-mRNA-1:cds;Parent=Msa0028790-mRNA-1
chr1_1 CDS 53845452 53845576 53845452 ID=Msa0028790-mRNA-1:cds;Parent=Msa0028790-mRNA-1
Gene Sequence

>Msa0028790

ATGGCATTTAAAGGGTATATCCTATGTCTAGTTATGGTCGTAGTGGCCAATATGTGTACTCAAAATGAAGCTCAATCAGGTTGTACATCTACACTCACTAGTTTGAGCCCTTGTCTAAACTACATAATGGGCAGTTCTTCAAATCCATCATCTTCATGTTGCTCACAACTTTCAACTGTAGTCCAATCTTCTCCACAGTGTCTATGCTCCCTCCTTAATGGTGGTGGTTCAACTTTTGGAATCACTATAAATCAAACACTTGCTCTGTCTCTCCCTTCTGCTTGTAAGGTTCAAACTCCACCGGTTAGCCAGTGCAAAGGTGGCAATGCACCAACATCTCCTTCAAGTTCAACTTCTTCAATTGGTTCACCTGTTGGTGCTCCAACTGAATCACCTGAAGGCACAATAACACCTTCTGCTTCAGACTTTCCCTCATCCTCAGGTGCAGGATCAAAAAGCGTCCCATCAACAGACGCTGGCTCGTCTGATGGAAGCGCTATTAAAGTCTCGTTCCACTTTGTTCTTTCCCTTCTTGCCATTGTGTTTTTTGTTGTCACCAAGTTTTGA

Protein sequence

>Msa0028790

MAFKGYILCLVMVVVANMCTQNEAQSGCTSTLTSLSPCLNYIMGSSSNPSSSCCSQLSTVVQSSPQCLCSLLNGGGSTFGITINQTLALSLPSACKVQTPPVSQCKGGNAPTSPSSSTSSIGSPVGAPTESPEGTITPSASDFPSSSGAGSKSVPSTDAGSSDGSAIKVSFHFVLSLLAIVFFVVTKF*