AlfalfaGEDB | Msa0033920

M. sativa cultivar zhongmu-4 / Msa0033920

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0033920	sp\|Q8RWW0\|ALE2_ARATH	66.667	117	39	0	255	371	503	619	2.35e-42	162

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0033920	Msa0034060	0.941266	5.610195e-101	-8.615850e-47
Msa0033920	Msa0128080	0.859336	4.437115e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0033920	MtrunA17_Chr1g0194541	90.875	263	18	2	1	263	1	257	3.20e-169	489
Msa0033920	MtrunA17_Chr1g0194541	93.443	183	12	0	255	437	453	635	1.56e-116	354
Msa0033920	MtrunA17_Chr7g0270121	79.891	184	36	1	255	437	542	725	8.34e-94	298
Msa0033920	MtrunA17_Chr7g0270121	60.829	217	84	1	43	258	124	340	4.04e-82	267
Msa0033920	MtrunA17_Chr7g0262521	69.027	113	35	0	264	376	515	627	8.80e-44	163
Msa0033920	MtrunA17_Chr2g0299301	70.370	108	32	0	264	371	1229	1336	1.90e-42	160
Msa0033920	MtrunA17_Chr1g0183041	61.789	123	47	0	257	379	513	635	3.82e-42	158
Msa0033920	MtrunA17_Chr8g0342071	64.815	108	38	0	264	371	619	726	2.97e-38	147
Msa0033920	MtrunA17_Chr4g0071221	60.000	125	50	0	252	376	697	821	1.94e-36	142
Msa0033920	MtrunA17_Chr2g0320111	58.929	112	46	0	257	368	259	370	4.83e-35	134

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0033920	AT4G02010.1	80.899	178	33	1	261	437	548	725	9.42e-94	298
Msa0033920	AT4G02010.1	54.464	224	101	1	44	267	125	347	1.86e-74	247
Msa0033920	AT2G20300.1	66.667	117	39	0	255	371	503	619	2.38e-43	162
Msa0033920	AT5G56890.1	67.593	108	35	0	264	371	893	1000	1.22e-38	149
Msa0033920	AT2G17220.1	44.156	154	81	2	255	406	254	404	3.30e-33	129
Msa0033920	AT2G17220.2	44.805	154	80	3	255	406	253	403	3.45e-33	129
Msa0033920	AT1G54820.1	55.556	108	48	0	261	368	321	428	4.79e-33	130
Msa0033920	AT3G58690.1	55.263	114	51	0	255	368	250	363	2.61e-32	127
Msa0033920	AT5G13160.1	41.892	148	81	2	264	408	256	401	4.57e-32	127
Msa0033920	AT5G38560.1	48.529	136	67	1	264	396	507	642	5.67e-32	129

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Find 149 sgRNAs with CRISPR-Local

Find 383 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCGATGTTTATAGCTA+TGG	0.404915	1_1:-61806408	Msa0033920:CDS
AAACACACAAGATACACACA+GGG	0.735502	1_1:-61810282	Msa0033920:five_prime_UTR
AAACCTCCAACTCCTTCTAA+AGG	0.473623	1_1:-61808825	Msa0033920:intron
AAAGACATGGTGTCAAAACG+AGG	0.676572	1_1:-61809164	Msa0033920:CDS
AAAGCGATGTTTATAGCTAT+GGG	0.449088	1_1:-61806407	Msa0033920:CDS
AAATGGCAGAGGACTGTCTC+AGG	0.485905	1_1:+61805904	None:intergenic
AACAGGACTGTGGTTTACCA+TGG	0.635545	1_1:+61805569	None:intergenic
AACCTTATGAAATAAAAGGG+AGG	0.645245	1_1:+61808902	None:intergenic
AACTTGCTGATCCAAGGCTT+GGG	0.340667	1_1:-61806100	Msa0033920:CDS
AAGCGATGTTTATAGCTATG+GGG	0.649374	1_1:-61806406	Msa0033920:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGGATTCAAAAAGGAAAAG+GGG	+	chr1_1:61805573-61805592	None:intergenic	30.0%
GATTGGATTCAAAAAGGAAA+AGG	+	chr1_1:61805575-61805594	None:intergenic	30.0%
ATTACCCATAGAAGAAAAAG+GGG	+	chr1_1:61805633-61805652	None:intergenic	30.0%
ACAGTGTTTATAGTTGAATG+TGG	-	chr1_1:61805814-61805833	Msa0033920:CDS	30.0%
CACTAATTAACTCCCATTAA+CGG	+	chr1_1:61806082-61806101	None:intergenic	30.0%
GAGGGACTAAAAATGAAAAT+TGG	-	chr1_1:61806161-61806180	Msa0033920:intron	30.0%
AATTGCTAACAGTAAGATGT+TGG	-	chr1_1:61806234-61806253	Msa0033920:intron	30.0%
AAAATCAAGAATGCTAGCAA+AGG	-	chr1_1:61806446-61806465	Msa0033920:CDS	30.0%
AAATCAAGAATGCTAGCAAA+GGG	-	chr1_1:61806447-61806466	Msa0033920:CDS	30.0%
AGATTGTTGTGAAAAAGACA+TGG	-	chr1_1:61806570-61806589	Msa0033920:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	61805385	61810384	61805385	ID=Msa0033920;Name=Msa0033920
chr1_1	mRNA	61805385	61810384	61805385	ID=Msa0033920-mRNA-1;Parent=Msa0033920;Name=Msa0033920-mRNA-1;_AED=0.15;_eAED=0.15;_QI=259\|0.8\|0.83\|1\|0.8\|1\|6\|397\|437
chr1_1	exon	61805385	61806159	61805385	ID=Msa0033920-mRNA-1:exon:19375;Parent=Msa0033920-mRNA-1
chr1_1	exon	61806326	61806473	61806326	ID=Msa0033920-mRNA-1:exon:19374;Parent=Msa0033920-mRNA-1
chr1_1	exon	61807803	61808013	61807803	ID=Msa0033920-mRNA-1:exon:19373;Parent=Msa0033920-mRNA-1
chr1_1	exon	61808826	61809197	61808826	ID=Msa0033920-mRNA-1:exon:19372;Parent=Msa0033920-mRNA-1
chr1_1	exon	61809820	61810020	61809820	ID=Msa0033920-mRNA-1:exon:19371;Parent=Msa0033920-mRNA-1
chr1_1	exon	61810122	61810384	61810122	ID=Msa0033920-mRNA-1:exon:19370;Parent=Msa0033920-mRNA-1
chr1_1	five_prime_UTR	61810126	61810384	61810126	ID=Msa0033920-mRNA-1:five_prime_utr;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61810122	61810125	61810122	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61809820	61810020	61809820	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61808826	61809197	61808826	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61807803	61808013	61807803	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61806326	61806473	61806326	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	CDS	61805782	61806159	61805782	ID=Msa0033920-mRNA-1:cds;Parent=Msa0033920-mRNA-1
chr1_1	three_prime_UTR	61805385	61805781	61805385	ID=Msa0033920-mRNA-1:three_prime_utr;Parent=Msa0033920-mRNA-1

Gene Sequence

>Msa0033920

ATGGGTGAGTTTGAAATGGGTCAAGGTCAATGCATATTGCTGAAAAGAGTGTTTGTGTTATTATTTAGTACAGTGTTTATAGTTGAATGTGGTGCATATGATACAACACTAGAAGCTTCTCATTTATCTATTTCTCCTATGCATTCTGTTGCTGGTTCAATTCCTGCTGCTATGGCTCATCCACCATTGTCCCCTTATCTTTCCAATTGTTGTGAAAAAGACATGGTGTCAAAACGAGGTAGTGAAGGCTGCCATTGTGTTTATCCTGTAAAGTTAGACCTTCTTCTTTTGAATGTCTCGGAAAACCCCGACTGGAATCTTTTTCTTGATGAATTGGCCGCACAATTGGGGATGCGTGCTACACAAATTGAGCTGATAAACTTTTATGTACACAGCTTATTGACTTTGAATATATCAATGTATATCACTCCGCGAAAAGAGATCAGTTTCTCTGCCAAGGAAGCATCTAAAATAAACTCCTCCCTTTTATTTCATAAGGTTCGACTAGACAGTAGATTCGTGGGCGATTACAGGGTTCTCAATTTGACCTGGTTTAAACCTCCAACTCCTTCTAAAGCTCCTACTTTTGCTACATCAAATGTGAAAACTCCTGAGAGACAAGTACCTACAGCTACATCATCGAGTACTTCAGACAAGGGAAGGCATTCAAATTTGCTTGTTATCCTTGGAATTGTTACTGGGATACTCATCATGTCCATTATCTGTGTACTTATACTTTGTTTATGCACGCTGAGGCCAAAAACAAAAAGACCTACAGAAACTGGCAAGTATGTAGCACCTGAGTATGCTATGACTGGACACCTACTCGTTAAAAGCGATGTTTATAGCTATGGGGTTGTCTTGTTAGAATTGTTGACCGGAAGGAAACCTGTGGAAATGTCACAGCCAACTGGACAAGAGAATCTTGTAACTTGGGCAAGGCCAATTCTTAGAGTTAAGGAGCAGTTAGAAGAACTTGCTGATCCAAGGCTTGGGGGAAGGTATCCAAAGGAAGATTTTTTCCGTGTTTGCACTATTGCTGCAGCCTGTGTTGCACCTGAAGCAAGTCAACGACCTACAATGGGGGAAGTGGTACAGTCACTTAAAATGGTTCAGCGCATCACAGAAAATCATGATCCTGCATTACCGTCATCCAATACAAGGCCCAACCTGAGACAGTCCTCTGCCATTTATGACTCTGATGGGACATCTTCGATGTTTTCTTCTGGTCCTTACTCTGGTCTAAGTGCCTTTGACTATGACAACACTTCTAGGACAGCTGTTTTCTCTGAAGATCTTCATGAAGGACGATGA

Protein sequence

>Msa0033920

MGEFEMGQGQCILLKRVFVLLFSTVFIVECGAYDTTLEASHLSISPMHSVAGSIPAAMAHPPLSPYLSNCCEKDMVSKRGSEGCHCVYPVKLDLLLLNVSENPDWNLFLDELAAQLGMRATQIELINFYVHSLLTLNISMYITPRKEISFSAKEASKINSSLLFHKVRLDSRFVGDYRVLNLTWFKPPTPSKAPTFATSNVKTPERQVPTATSSSTSDKGRHSNLLVILGIVTGILIMSIICVLILCLCTLRPKTKRPTETGKYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVEMSQPTGQENLVTWARPILRVKEQLEELADPRLGGRYPKEDFFRVCTIAAACVAPEASQRPTMGEVVQSLKMVQRITENHDPALPSSNTRPNLRQSSAIYDSDGTSSMFSSGPYSGLSAFDYDNTSRTAVFSEDLHEGR*