AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0074570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074570 MtrunA17_Chr1g0188791 93.220 118 6 2 13 130 15 130 7.41e-72 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0074570 AT1G28150.1 66.216 74 23 1 59 130 50 123 3.19e-28 100
Msa0074570 AT1G28150.2 66.216 74 23 1 59 130 50 123 3.19e-28 100

Find 47 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCTTAATGAATATTAT+TGG 0.190749 1_2:-49235918 None:intergenic
AAAACCTATGCTTCTGGTTC+TGG 0.247087 1_2:-49232845 None:intergenic
CCAAATCAATCCGATTCTTA+TGG 0.252356 1_2:-49232585 None:intergenic
TTCTTAGGTGGGATATTTGC+TGG 0.305119 1_2:+49235385 Msa0074570:CDS
CATATAGGACATAATAGTTC+TGG 0.312454 1_2:+49235658 Msa0074570:three_prime_UTR
CAGGAACCAGTGGCATTCTT+AGG 0.325742 1_2:+49235370 Msa0074570:intron
ATAATTAAAACCTATGCTTC+TGG 0.329492 1_2:-49232851 None:intergenic
TGGTAAATTGTCAACATTCA+TGG 0.342708 1_2:+49235706 Msa0074570:three_prime_UTR
ATTGTTTGCACCATTTGTTT+TGG 0.357688 1_2:+49235825 Msa0074570:three_prime_UTR
CAAATCAATCCGATTCTTAT+GGG 0.373950 1_2:-49232584 None:intergenic
CCATAAGAATCGGATTGATT+TGG 0.397964 1_2:+49232585 Msa0074570:five_prime_UTR
AGTAGTTGATTCTGCTGGAT+TGG 0.400556 1_2:-49235494 None:intergenic
ATTCCCTTCGAGTGGGTAAT+CGG 0.403183 1_2:+49232872 Msa0074570:CDS
TTAAAGATATCAAAGTTGTT+TGG 0.418131 1_2:+49235880 Msa0074570:three_prime_UTR
TGAAATTGAAGCAGAAATGT+AGG 0.423767 1_2:+49232785 Msa0074570:intron
AATTGTCAACATTCATGGCT+TGG 0.447143 1_2:+49235711 Msa0074570:three_prime_UTR
GCTTCAGTAGTTGATTCTGC+TGG 0.449685 1_2:-49235499 None:intergenic
TTGACGAGAGGGTCTTGAGC+AGG 0.477747 1_2:-49232922 None:intergenic
TATCACCATCATCAATTTCG+CGG 0.480081 1_2:+49232812 Msa0074570:CDS
TATTCAATTTCAATCTCTTG+TGG 0.483856 1_2:-49235526 None:intergenic
ACAAATTATTATTGTGCTGT+TGG 0.485885 1_2:-49235594 None:intergenic
GGTTAAACTGCCAAAACAAA+TGG 0.497837 1_2:-49235835 None:intergenic
ATGAGGAACCTCTGAAGGAA+TGG 0.505614 1_2:+49235428 Msa0074570:CDS
AAAACAGCGCCCATAAGAAT+CGG 0.507763 1_2:+49232575 None:intergenic
TTTAAGGCTTGATTTGAATG+AGG 0.510298 1_2:+49235411 Msa0074570:CDS
GACAAGAATAACTATATAGC+AGG 0.512346 1_2:-49235856 None:intergenic
TGAGGAACCTCTGAAGGAAT+GGG 0.523835 1_2:+49235429 Msa0074570:CDS
CTAGTGTGGTTGTTGCGAGT+TGG 0.524741 1_2:+49232614 Msa0074570:five_prime_UTR
TCTTCAATTGTCATGCACTC+AGG 0.530095 1_2:+49232687 Msa0074570:CDS
CTCGTAACCCATTCCTTCAG+AGG 0.539677 1_2:-49235436 None:intergenic
TTCTAATCTGCAGGAACCAG+TGG 0.542541 1_2:+49235360 Msa0074570:intron
GAACCAGTGGCATTCTTAGG+TGG 0.545711 1_2:+49235373 Msa0074570:CDS
CGACTCCGCGAAATTGATGA+TGG 0.562731 1_2:-49232817 None:intergenic
TTTGAATGAGGAACCTCTGA+AGG 0.563194 1_2:+49235423 Msa0074570:CDS
TGACAGCTTCTTTGACGAGA+GGG 0.564005 1_2:-49232933 None:intergenic
TTTAATTATTCCCTTCGAGT+GGG 0.566584 1_2:+49232865 Msa0074570:CDS
ATCCCACCTAAGAATGCCAC+TGG 0.574031 1_2:-49235376 None:intergenic
TGATTTGGTAGCTGCTAGTG+TGG 0.593135 1_2:+49232600 Msa0074570:five_prime_UTR
CTCTGAAGGAATGGGTTACG+AGG 0.596910 1_2:+49235437 Msa0074570:CDS
AACCAGTGGCATTCTTAGGT+GGG 0.598806 1_2:+49235374 Msa0074570:CDS
TGAGTGCATGACAATTGAAG+AGG 0.605166 1_2:-49232685 None:intergenic
TCTCGTCAAAGAAGCTGTCA+AGG 0.609141 1_2:+49232936 Msa0074570:CDS
GACTCCGATTACCCACTCGA+AGG 0.630206 1_2:-49232876 None:intergenic
TTGACAGCTTCTTTGACGAG+AGG 0.633404 1_2:-49232934 None:intergenic
ACTCCGATTACCCACTCGAA+GGG 0.634328 1_2:-49232875 None:intergenic
ATCACCAGAACCAGAAGCAT+AGG 0.634601 1_2:+49232841 Msa0074570:CDS
GTTGTTGCGAGTTGGAACAG+AGG 0.730829 1_2:+49232622 Msa0074570:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTAGCTTATTTAT+TGG + chr1_2:49233027-49233046 Msa0074570:intron 10.0%
!! AGAAAAAATTGTTAATAATA+TGG + chr1_2:49233811-49233830 Msa0074570:intron 10.0%
!!! TGAGATTTTAAATTAATATA+AGG + chr1_2:49233940-49233959 Msa0074570:intron 10.0%
!!! AATTAATATAAGGATAATTT+TGG + chr1_2:49233950-49233969 Msa0074570:intron 10.0%
!! AAAATCTTAATGAATATTAT+TGG - chr1_2:49235921-49235940 None:intergenic 10.0%
!! AGGAATAATAGTAATAATAA+CGG - chr1_2:49232714-49232733 None:intergenic 15.0%
!!! ATTTTAATTAAAAGTGTGTT+TGG + chr1_2:49233633-49233652 Msa0074570:intron 15.0%
!!! CCTTATTTAATTAAGTTTAA+GGG - chr1_2:49233704-49233723 None:intergenic 15.0%
!!! TCCTTATTTAATTAAGTTTA+AGG - chr1_2:49233705-49233724 None:intergenic 15.0%
!!! TTTTACTATTATTAGGTTAT+AGG - chr1_2:49233865-49233884 None:intergenic 15.0%
!!! AAACAACTTTTACTATTATT+AGG - chr1_2:49233872-49233891 None:intergenic 15.0%
!! TAATAATAAATGTTGTTACA+AGG + chr1_2:49233910-49233929 Msa0074570:intron 15.0%
!! GAGAAATAAATAAATTGTAA+TGG - chr1_2:49234382-49234401 None:intergenic 15.0%
!!! TCAAGTTATTCTAAAATTTT+GGG - chr1_2:49234822-49234841 None:intergenic 15.0%
!!! TTCAAGTTATTCTAAAATTT+TGG - chr1_2:49234823-49234842 None:intergenic 15.0%
!!! TATTTTCAAGTGTTTTATTT+TGG + chr1_2:49235780-49235799 Msa0074570:three_prime_UTR 15.0%
!! AGCTAAAAATAAATACAAGA+AGG - chr1_2:49233020-49233039 None:intergenic 20.0%
!! ACAAAATTAACAACTAACAT+TGG + chr1_2:49233330-49233349 Msa0074570:intron 20.0%
!!! ATACAAATAGAGTTTTATGT+CGG + chr1_2:49233409-49233428 Msa0074570:intron 20.0%
!! CCCTTAAACTTAATTAAATA+AGG + chr1_2:49233701-49233720 Msa0074570:intron 20.0%
!!! TTTGGTTCAATTTTTAGAAA+GGG - chr1_2:49234006-49234025 None:intergenic 20.0%
!!! GATTTTTAATTTGAAGTGTT+TGG - chr1_2:49234024-49234043 None:intergenic 20.0%
!!! ATTTATTTCTCAGTTCTTTT+TGG + chr1_2:49234391-49234410 Msa0074570:intron 20.0%
!!! TTTATTTCTCAGTTCTTTTT+GGG + chr1_2:49234392-49234411 Msa0074570:intron 20.0%
!!! TTTGAAGTTCTGTTATTTTT+GGG - chr1_2:49234436-49234455 None:intergenic 20.0%
!! TGATTCTACTATGATTTATT+AGG - chr1_2:49234532-49234551 None:intergenic 20.0%
!! TTGATAAATAAAGAAGTTCA+TGG - chr1_2:49235026-49235045 None:intergenic 20.0%
!!! CAATTTTATCACAATTCTAT+AGG + chr1_2:49235251-49235270 Msa0074570:intron 20.0%
!!! TGCAAGTTTTAAAGAAATAA+AGG - chr1_2:49235561-49235580 None:intergenic 20.0%
!!! TTATCACACATTTTTCATAT+AGG + chr1_2:49235643-49235662 Msa0074570:three_prime_UTR 20.0%
!!! TTTTGGAAATCAAATACAAA+AGG + chr1_2:49235797-49235816 Msa0074570:three_prime_UTR 20.0%
!! TTAAAGATATCAAAGTTGTT+TGG + chr1_2:49235880-49235899 Msa0074570:three_prime_UTR 20.0%
! ATAATTAAAACCTATGCTTC+TGG - chr1_2:49232854-49232873 None:intergenic 25.0%
! ATGTTCAGCTTATTTCAATT+TGG + chr1_2:49233061-49233080 Msa0074570:intron 25.0%
! GCTATATATAAGAGAAAGTT+TGG + chr1_2:49233107-49233126 Msa0074570:intron 25.0%
!! AGCTGATTTAGTTCAAAATT+TGG - chr1_2:49233157-49233176 None:intergenic 25.0%
!!! TTGGTTCAATTTTTAGAAAG+GGG - chr1_2:49234005-49234024 None:intergenic 25.0%
!!! GTTTGGTTCAATTTTTAGAA+AGG - chr1_2:49234007-49234026 None:intergenic 25.0%
! TAAACATAACCTGACTTATA+GGG - chr1_2:49234086-49234105 None:intergenic 25.0%
! ATAAACATAACCTGACTTAT+AGG - chr1_2:49234087-49234106 None:intergenic 25.0%
! TTGTTCATGAATCAATAGTA+AGG - chr1_2:49234116-49234135 None:intergenic 25.0%
!! AATTTCAGTTTTACACCAAA+AGG - chr1_2:49234247-49234266 None:intergenic 25.0%
!!! TTCTTTTTGGGATAAAAAGA+GGG + chr1_2:49234404-49234423 Msa0074570:intron 25.0%
!!! CTTTGAAGTTCTGTTATTTT+TGG - chr1_2:49234437-49234456 None:intergenic 25.0%
! TGTCTTAGTTAATATGGATT+AGG - chr1_2:49234558-49234577 None:intergenic 25.0%
!! TGATGATGTCTTAGTTAATA+TGG - chr1_2:49234564-49234583 None:intergenic 25.0%
! ACTACAAAGCTCTATAATAT+AGG + chr1_2:49234785-49234804 Msa0074570:intron 25.0%
! AATGAAAAAGTTCTCACAAA+GGG - chr1_2:49234950-49234969 None:intergenic 25.0%
!!! ATTGCTAGGCTTTAAAAATT+TGG + chr1_2:49235309-49235328 Msa0074570:intron 25.0%
! TATTCAATTTCAATCTCTTG+TGG - chr1_2:49235529-49235548 None:intergenic 25.0%
! ACAAATTATTATTGTGCTGT+TGG - chr1_2:49235597-49235616 None:intergenic 25.0%
CAAATCAATCCGATTCTTAT+GGG - chr1_2:49232587-49232606 None:intergenic 30.0%
TTCCTCTTTCTTACTCTTTA+TGG + chr1_2:49232729-49232748 Msa0074570:intron 30.0%
AACCATAAAGAGTAAGAAAG+AGG - chr1_2:49232734-49232753 None:intergenic 30.0%
TGAAATTGAAGCAGAAATGT+AGG + chr1_2:49232785-49232804 Msa0074570:intron 30.0%
!! TTTTAATTATTCCCTTCGAG+TGG + chr1_2:49232864-49232883 Msa0074570:CDS 30.0%
TTTAATTATTCCCTTCGAGT+GGG + chr1_2:49232865-49232884 Msa0074570:CDS 30.0%
TACAAGAAGGAGCTAAAATA+TGG - chr1_2:49233007-49233026 None:intergenic 30.0%
GAATATAAAAAATACGCCTC+AGG + chr1_2:49233083-49233102 Msa0074570:intron 30.0%
AAAATCTAACAAGATCAGCT+AGG + chr1_2:49233210-49233229 Msa0074570:intron 30.0%
AATTAAGCAATTACACTTGC+AGG - chr1_2:49233380-49233399 None:intergenic 30.0%
! ATTGATAACACATGAGACAT+GGG - chr1_2:49233477-49233496 None:intergenic 30.0%
! AATTGATAACACATGAGACA+TGG - chr1_2:49233478-49233497 None:intergenic 30.0%
!! AGGGAAGGAATTTTAACAAA+GGG + chr1_2:49233590-49233609 Msa0074570:intron 30.0%
!! TTCAATTTTTAGAAAGGGGA+GGG - chr1_2:49234001-49234020 None:intergenic 30.0%
TATCAGCATATAAAACTGTC+TGG + chr1_2:49234142-49234161 Msa0074570:intron 30.0%
! TTTTCAGTGGTAACATAACA+TGG - chr1_2:49234203-49234222 None:intergenic 30.0%
! TTTTGCATCACAATTTTCAG+TGG - chr1_2:49234216-49234235 None:intergenic 30.0%
!!! GTTCTTTTTGGGATAAAAAG+AGG + chr1_2:49234403-49234422 Msa0074570:intron 30.0%
AACTTCAAAGCAATTAAACG+TGG + chr1_2:49234447-49234466 Msa0074570:intron 30.0%
GAATAGAATGTTGCTAGAAA+TGG - chr1_2:49234634-49234653 None:intergenic 30.0%
!! TATAGAGCTTTGTAGTGTTT+TGG - chr1_2:49234781-49234800 None:intergenic 30.0%
! CTAATCATGGACTGTAATTT+AGG + chr1_2:49234908-49234927 Msa0074570:intron 30.0%
! ATGGACTGTAATTTAGGAAT+TGG + chr1_2:49234914-49234933 Msa0074570:intron 30.0%
GAATGAAAAAGTTCTCACAA+AGG - chr1_2:49234951-49234970 None:intergenic 30.0%
! TCATACCAGTGTTATTACAA+GGG - chr1_2:49235074-49235093 None:intergenic 30.0%
! TTCATACCAGTGTTATTACA+AGG - chr1_2:49235075-49235094 None:intergenic 30.0%
TATGTTAACAAGTTGCTATC+TGG + chr1_2:49235181-49235200 Msa0074570:intron 30.0%
!! TCTTGTTATTTCTAATCTGC+AGG + chr1_2:49235351-49235370 Msa0074570:intron 30.0%
!! GGATATTTGCTGGTATTTTA+AGG + chr1_2:49235395-49235414 Msa0074570:CDS 30.0%
! TTTAAGGCTTGATTTGAATG+AGG + chr1_2:49235411-49235430 Msa0074570:CDS 30.0%
CATATAGGACATAATAGTTC+TGG + chr1_2:49235658-49235677 Msa0074570:three_prime_UTR 30.0%
! CTTTAAAAAGACGAAGACAA+AGG - chr1_2:49235685-49235704 None:intergenic 30.0%
!! TGTCTTCGTCTTTTTAAAGA+TGG + chr1_2:49235686-49235705 Msa0074570:three_prime_UTR 30.0%
TGGTAAATTGTCAACATTCA+TGG + chr1_2:49235706-49235725 Msa0074570:three_prime_UTR 30.0%
! ATTGTTTGCACCATTTGTTT+TGG + chr1_2:49235825-49235844 Msa0074570:three_prime_UTR 30.0%
GACAAGAATAACTATATAGC+AGG - chr1_2:49235859-49235878 None:intergenic 30.0%
CCAAATCAATCCGATTCTTA+TGG - chr1_2:49232588-49232607 None:intergenic 35.0%
CCATAAGAATCGGATTGATT+TGG + chr1_2:49232585-49232604 Msa0074570:five_prime_UTR 35.0%
TATCACCATCATCAATTTCG+CGG + chr1_2:49232812-49232831 Msa0074570:CDS 35.0%
TCTCTTATATATAGCACCTG+AGG - chr1_2:49233102-49233121 None:intergenic 35.0%
!! GAGGGAAGGAATTTTAACAA+AGG + chr1_2:49233589-49233608 Msa0074570:intron 35.0%
!! GGGAAGGAATTTTAACAAAG+GGG + chr1_2:49233591-49233610 Msa0074570:intron 35.0%
!! AAGGAATTTTAACAAAGGGG+AGG + chr1_2:49233594-49233613 Msa0074570:intron 35.0%
!! AGGAATTTTAACAAAGGGGA+GGG + chr1_2:49233595-49233614 Msa0074570:intron 35.0%
! AAAAGTGTGTTTGGTTCACA+AGG + chr1_2:49233642-49233661 Msa0074570:intron 35.0%
! AAAGTGTGTTTGGTTCACAA+GGG + chr1_2:49233643-49233662 Msa0074570:intron 35.0%
!! TCAATTTTTAGAAAGGGGAG+GGG - chr1_2:49234000-49234019 None:intergenic 35.0%
!! GTTCAATTTTTAGAAAGGGG+AGG - chr1_2:49234002-49234021 None:intergenic 35.0%
AAACTGTCTGGTGGAAAATA+TGG + chr1_2:49234154-49234173 Msa0074570:intron 35.0%
! TGCAAAAGTCATGTTCCTTT+TGG + chr1_2:49234229-49234248 Msa0074570:intron 35.0%
AAAGCAATTAAACGTGGAGT+AGG + chr1_2:49234453-49234472 Msa0074570:intron 35.0%
GTTGGATTAAGCTTAACATG+GGG - chr1_2:49234868-49234887 None:intergenic 35.0%
GGTTGGATTAAGCTTAACAT+GGG - chr1_2:49234869-49234888 None:intergenic 35.0%
TTAAGCTTAATCCAACCCAA+AGG + chr1_2:49234872-49234891 Msa0074570:intron 35.0%
TACAGTCCATGATTAGATTG+TGG - chr1_2:49234904-49234923 None:intergenic 35.0%
TAAAGAAGTTCATGGTGAAC+TGG - chr1_2:49235018-49235037 None:intergenic 35.0%
ATGAACCCTTGTAATAACAC+TGG + chr1_2:49235066-49235085 Msa0074570:intron 35.0%
TTAACAAGTTGCTATCTGGA+TGG + chr1_2:49235185-49235204 Msa0074570:intron 35.0%
TAGCTACATCATAAGAACCA+GGG - chr1_2:49235227-49235246 None:intergenic 35.0%
AATTGTCAACATTCATGGCT+TGG + chr1_2:49235711-49235730 Msa0074570:three_prime_UTR 35.0%
GGTTAAACTGCCAAAACAAA+TGG - chr1_2:49235838-49235857 None:intergenic 35.0%
TGAGTGCATGACAATTGAAG+AGG - chr1_2:49232688-49232707 None:intergenic 40.0%
TCTTCAATTGTCATGCACTC+AGG + chr1_2:49232687-49232706 Msa0074570:CDS 40.0%
!! AAAACCTATGCTTCTGGTTC+TGG - chr1_2:49232848-49232867 None:intergenic 40.0%
ATCTAACAAGATCAGCTAGG+AGG + chr1_2:49233213-49233232 Msa0074570:intron 40.0%
TCTAACAAGATCAGCTAGGA+GGG + chr1_2:49233214-49233233 Msa0074570:intron 40.0%
GTTAGAGAGTGGAGTGATAA+CGG + chr1_2:49233270-49233289 Msa0074570:intron 40.0%
TTAGAGAGTGGAGTGATAAC+GGG + chr1_2:49233271-49233290 Msa0074570:intron 40.0%
!! GGAATTTTAACAAAGGGGAG+GGG + chr1_2:49233596-49233615 Msa0074570:intron 40.0%
! AAGTGTGTTTGGTTCACAAG+GGG + chr1_2:49233644-49233663 Msa0074570:intron 40.0%
TAACAACACGATGTAGACGA+TGG + chr1_2:49233759-49233778 Msa0074570:intron 40.0%
CGATGTAGACGATGGAATAA+CGG + chr1_2:49233767-49233786 Msa0074570:intron 40.0%
!!! TTTGGTTCGAGAGTTTTGGA+GGG - chr1_2:49234059-49234078 None:intergenic 40.0%
TCCAAAACTCTCGAACCAAA+CGG + chr1_2:49234059-49234078 Msa0074570:intron 40.0%
CAGCATATAAAACTGTCTGG+TGG + chr1_2:49234145-49234164 Msa0074570:intron 40.0%
CACATGAGTCATGACAGAAA+TGG - chr1_2:49234277-49234296 None:intergenic 40.0%
ACAAGCTGCTGTAGAAAAAC+AGG + chr1_2:49234334-49234353 Msa0074570:intron 40.0%
TTTCTCTTAGCTGATCTCGT+TGG - chr1_2:49234483-49234502 None:intergenic 40.0%
GAGAATTTCCATGCTCTAGT+TGG - chr1_2:49234510-49234529 None:intergenic 40.0%
GACATCATCAGTACATGAGT+TGG + chr1_2:49234574-49234593 Msa0074570:intron 40.0%
AACGATTTCTCCAATGACTG+TGG - chr1_2:49234680-49234699 None:intergenic 40.0%
AGTCATTGGAGAAATCGTTG+AGG + chr1_2:49234681-49234700 Msa0074570:intron 40.0%
TAACATGGGGCTTCTAATAG+TGG - chr1_2:49234855-49234874 None:intergenic 40.0%
GGGTTGGATTAAGCTTAACA+TGG - chr1_2:49234870-49234889 None:intergenic 40.0%
AGGTAGCCACAATCTAATCA+TGG + chr1_2:49234895-49234914 Msa0074570:intron 40.0%
TACATCATAAGAACCAGGGA+TGG - chr1_2:49235223-49235242 None:intergenic 40.0%
GTAGCTACATCATAAGAACC+AGG - chr1_2:49235228-49235247 None:intergenic 40.0%
TTCTTAGGTGGGATATTTGC+TGG + chr1_2:49235385-49235404 Msa0074570:CDS 40.0%
TTTGAATGAGGAACCTCTGA+AGG + chr1_2:49235423-49235442 Msa0074570:CDS 40.0%
! AGTAGTTGATTCTGCTGGAT+TGG - chr1_2:49235497-49235516 None:intergenic 40.0%
!! TGATTTGGTAGCTGCTAGTG+TGG + chr1_2:49232600-49232619 Msa0074570:five_prime_UTR 45.0%
! ATCACCAGAACCAGAAGCAT+AGG + chr1_2:49232841-49232860 Msa0074570:CDS 45.0%
ATTCCCTTCGAGTGGGTAAT+CGG + chr1_2:49232872-49232891 Msa0074570:CDS 45.0%
TGACAGCTTCTTTGACGAGA+GGG - chr1_2:49232936-49232955 None:intergenic 45.0%
TTGACAGCTTCTTTGACGAG+AGG - chr1_2:49232937-49232956 None:intergenic 45.0%
TCTCGTCAAAGAAGCTGTCA+AGG + chr1_2:49232936-49232955 Msa0074570:CDS 45.0%
CTAACAAGATCAGCTAGGAG+GGG + chr1_2:49233215-49233234 Msa0074570:intron 45.0%
GAAGTGGAAGAGTTAGAGAG+TGG + chr1_2:49233259-49233278 Msa0074570:intron 45.0%
! TAGAGAGTGGAGTGATAACG+GGG + chr1_2:49233272-49233291 Msa0074570:intron 45.0%
GGGTTCTCGGATTTGATTCT+CGG + chr1_2:49233292-49233311 Msa0074570:intron 45.0%
TTAGTTGTGTCCTGTGACCT+TGG + chr1_2:49233520-49233539 Msa0074570:intron 45.0%
ATTCTCGAACGTCACAACCA+AGG - chr1_2:49233540-49233559 None:intergenic 45.0%
GGTTGTGACGTTCGAGAATT+AGG + chr1_2:49233541-49233560 Msa0074570:intron 45.0%
TGTGACGTTCGAGAATTAGG+AGG + chr1_2:49233544-49233563 Msa0074570:intron 45.0%
GTGACGTTCGAGAATTAGGA+GGG + chr1_2:49233545-49233564 Msa0074570:intron 45.0%
TGACGTTCGAGAATTAGGAG+GGG + chr1_2:49233546-49233565 Msa0074570:intron 45.0%
!! ATTTTAACAAAGGGGAGGGG+AGG + chr1_2:49233599-49233618 Msa0074570:intron 45.0%
! TTTTAACAAAGGGGAGGGGA+GGG + chr1_2:49233600-49233619 Msa0074570:intron 45.0%
!! TTTTTAGAAAGGGGAGGGGA+GGG - chr1_2:49233996-49234015 None:intergenic 45.0%
!! ATTTTTAGAAAGGGGAGGGG+AGG - chr1_2:49233997-49234016 None:intergenic 45.0%
!!! TTGGTTCGAGAGTTTTGGAG+GGG - chr1_2:49234058-49234077 None:intergenic 45.0%
!! GTTTGGTTCGAGAGTTTTGG+AGG - chr1_2:49234060-49234079 None:intergenic 45.0%
!! TCCGTTTGGTTCGAGAGTTT+TGG - chr1_2:49234063-49234082 None:intergenic 45.0%
GAAACGAACCAACTAGAGCA+TGG + chr1_2:49234499-49234518 Msa0074570:intron 45.0%
! CATGAGTTGGCTTCACGATA+TGG + chr1_2:49234587-49234606 Msa0074570:intron 45.0%
ATGTTGCTAGAAATGGCGAG+AGG - chr1_2:49234627-49234646 None:intergenic 45.0%
! TGTAGTTAGCCTGGTCTGAT+GGG - chr1_2:49234752-49234771 None:intergenic 45.0%
! TTGTAGTTAGCCTGGTCTGA+TGG - chr1_2:49234753-49234772 None:intergenic 45.0%
TGGAGAGATTGTAGTTAGCC+TGG - chr1_2:49234761-49234780 None:intergenic 45.0%
AGCTTAATCCAACCCAAAGG+AGG + chr1_2:49234875-49234894 Msa0074570:intron 45.0%
AGATTGTGGCTACCTCCTTT+GGG - chr1_2:49234890-49234909 None:intergenic 45.0%
TAGATTGTGGCTACCTCCTT+TGG - chr1_2:49234891-49234910 None:intergenic 45.0%
! TGGTGAACTGGTGAGCTTTT+CGG - chr1_2:49235006-49235025 None:intergenic 45.0%
! GTAAGTTGACTTACCATCCC+TGG + chr1_2:49235207-49235226 Msa0074570:intron 45.0%
TTCTAATCTGCAGGAACCAG+TGG + chr1_2:49235360-49235379 Msa0074570:intron 45.0%
AACCAGTGGCATTCTTAGGT+GGG + chr1_2:49235374-49235393 Msa0074570:CDS 45.0%
! ATGAGGAACCTCTGAAGGAA+TGG + chr1_2:49235428-49235447 Msa0074570:CDS 45.0%
! TGAGGAACCTCTGAAGGAAT+GGG + chr1_2:49235429-49235448 Msa0074570:CDS 45.0%
! GCTTCAGTAGTTGATTCTGC+TGG - chr1_2:49235502-49235521 None:intergenic 45.0%
!!! TAAGTTTTTTTTTTTTTTAA+CGG - chr1_2:49233356-49233375 None:intergenic 5.0%
CTAGTGTGGTTGTTGCGAGT+TGG + chr1_2:49232614-49232633 Msa0074570:five_prime_UTR 50.0%
! GTTGTTGCGAGTTGGAACAG+AGG + chr1_2:49232622-49232641 Msa0074570:five_prime_UTR 50.0%
CGACTCCGCGAAATTGATGA+TGG - chr1_2:49232820-49232839 None:intergenic 50.0%
ACTCCGATTACCCACTCGAA+GGG - chr1_2:49232878-49232897 None:intergenic 50.0%
AGCTAGGAGGGGAATGAAGT+TGG + chr1_2:49233226-49233245 Msa0074570:intron 50.0%
!! AGTTGGCTTGGTGGTTGAAG+TGG + chr1_2:49233243-49233262 Msa0074570:intron 50.0%
!! TGGAGTGATAACGGGGTTCT+CGG + chr1_2:49233279-49233298 Msa0074570:intron 50.0%
GGGCACATGGCACAACTATA+TGG - chr1_2:49233457-49233476 None:intergenic 50.0%
ACACATGAGACATGGGCACA+TGG - chr1_2:49233470-49233489 None:intergenic 50.0%
AACGTCACAACCAAGGTCAC+AGG - chr1_2:49233533-49233552 None:intergenic 50.0%
GACGTTCGAGAATTAGGAGG+GGG + chr1_2:49233547-49233566 Msa0074570:intron 50.0%
TTCGAGAATTAGGAGGGGGA+GGG + chr1_2:49233551-49233570 Msa0074570:intron 50.0%
TTTAACAAAGGGGAGGGGAG+GGG + chr1_2:49233601-49233620 Msa0074570:intron 50.0%
! TGTGTTTGGTTCACAAGGGG+AGG + chr1_2:49233647-49233666 Msa0074570:intron 50.0%
! GTGTTTGGTTCACAAGGGGA+GGG + chr1_2:49233648-49233667 Msa0074570:intron 50.0%
! TGTTTGGTTCACAAGGGGAG+GGG + chr1_2:49233649-49233668 Msa0074570:intron 50.0%
!! TTTTAGAAAGGGGAGGGGAG+GGG - chr1_2:49233995-49234014 None:intergenic 50.0%
!!! TTCGAGAGTTTTGGAGGGGA+GGG - chr1_2:49234054-49234073 None:intergenic 50.0%
CCTGACTTATAGGGTCCGTT+TGG - chr1_2:49234077-49234096 None:intergenic 50.0%
CCAAACGGACCCTATAAGTC+AGG + chr1_2:49234074-49234093 Msa0074570:intron 50.0%
! CTAGAAATGGCGAGAGGTGT+AGG - chr1_2:49234621-49234640 None:intergenic 50.0%
TCTCCAATGACTGTGGAGCT+CGG - chr1_2:49234673-49234692 None:intergenic 50.0%
! GGTGAACTGGTGAGCTTTTC+GGG - chr1_2:49235005-49235024 None:intergenic 50.0%
CTAGCAATGAACGTTCCTGC+GGG - chr1_2:49235297-49235316 None:intergenic 50.0%
CCTAGCAATGAACGTTCCTG+CGG - chr1_2:49235298-49235317 None:intergenic 50.0%
CAGGAACCAGTGGCATTCTT+AGG + chr1_2:49235370-49235389 Msa0074570:intron 50.0%
GAACCAGTGGCATTCTTAGG+TGG + chr1_2:49235373-49235392 Msa0074570:CDS 50.0%
ATCCCACCTAAGAATGCCAC+TGG - chr1_2:49235379-49235398 None:intergenic 50.0%
CTCGTAACCCATTCCTTCAG+AGG - chr1_2:49235439-49235458 None:intergenic 50.0%
!! CTCTGAAGGAATGGGTTACG+AGG + chr1_2:49235437-49235456 Msa0074570:CDS 50.0%
GACTCCGATTACCCACTCGA+AGG - chr1_2:49232879-49232898 None:intergenic 55.0%
TTGACGAGAGGGTCTTGAGC+AGG - chr1_2:49232925-49232944 None:intergenic 55.0%
GGAGGGGAATGAAGTTGGCT+TGG + chr1_2:49233231-49233250 Msa0074570:intron 55.0%
!! GGGGAATGAAGTTGGCTTGG+TGG + chr1_2:49233234-49233253 Msa0074570:intron 55.0%
GTTCGAGAATTAGGAGGGGG+AGG + chr1_2:49233550-49233569 Msa0074570:intron 55.0%
TCGAGAATTAGGAGGGGGAG+GGG + chr1_2:49233552-49233571 Msa0074570:intron 55.0%
!!! TCGAGAGTTTTGGAGGGGAG+GGG - chr1_2:49234053-49234072 None:intergenic 55.0%
!!! GTTCGAGAGTTTTGGAGGGG+AGG - chr1_2:49234055-49234074 None:intergenic 55.0%
!! GCTCGGTGGAGTTGGTGAAA+TGG - chr1_2:49234656-49234675 None:intergenic 55.0%
AGCTCGAAACCCATCAGACC+AGG + chr1_2:49234740-49234759 Msa0074570:intron 55.0%
TGTGGCTACCTCCTTTGGGT+TGG - chr1_2:49234886-49234905 None:intergenic 55.0%
AAGCATTCTGTTCAGCCCGC+AGG + chr1_2:49235279-49235298 Msa0074570:intron 55.0%
CCGCAGGAACGTTCATTGCT+AGG + chr1_2:49235295-49235314 Msa0074570:intron 55.0%
AGAATTAGGAGGGGGAGGGG+AGG + chr1_2:49233555-49233574 Msa0074570:intron 60.0%
GAATTAGGAGGGGGAGGGGA+GGG + chr1_2:49233556-49233575 Msa0074570:intron 60.0%
AATTAGGAGGGGGAGGGGAG+GGG + chr1_2:49233557-49233576 Msa0074570:intron 60.0%
TTGGTTCACAAGGGGAGGGG+AGG + chr1_2:49233652-49233671 Msa0074570:intron 60.0%
TGGTTCACAAGGGGAGGGGA+GGG + chr1_2:49233653-49233672 Msa0074570:intron 60.0%
!!! AGTTTTGGAGGGGAGGGGAG+GGG - chr1_2:49234048-49234067 None:intergenic 60.0%
!!! GAGTTTTGGAGGGGAGGGGA+GGG - chr1_2:49234049-49234068 None:intergenic 60.0%
!!! AGAGTTTTGGAGGGGAGGGG+AGG - chr1_2:49234050-49234069 None:intergenic 60.0%
! ACTGTGGAGCTCGGTGGAGT+TGG - chr1_2:49234664-49234683 None:intergenic 60.0%
CCAATGACTGTGGAGCTCGG+TGG - chr1_2:49234670-49234689 None:intergenic 60.0%
CCACCGAGCTCCACAGTCAT+TGG + chr1_2:49234667-49234686 Msa0074570:intron 60.0%
AACAAAGGGGAGGGGAGGGG+AGG + chr1_2:49233604-49233623 Msa0074570:intron 65.0%
ACAAAGGGGAGGGGAGGGGA+GGG + chr1_2:49233605-49233624 Msa0074570:intron 65.0%
CAAAGGGGAGGGGAGGGGAG+GGG + chr1_2:49233606-49233625 Msa0074570:intron 70.0%
TAGGAGGGGGAGGGGAGGGG+AGG + chr1_2:49233560-49233579 Msa0074570:intron 75.0%
AGGAGGGGGAGGGGAGGGGA+GGG + chr1_2:49233561-49233580 Msa0074570:intron 75.0%
AGGGGAGGGGAGGGGAGGGA+AGG + chr1_2:49233575-49233594 Msa0074570:intron 75.0%
! GGAGGGGGAGGGGAGGGGAG+GGG + chr1_2:49233562-49233581 Msa0074570:intron 80.0%
! GGGGAGGGGAGGGGAGGGGA+GGG + chr1_2:49233566-49233585 Msa0074570:intron 80.0%
! GGGAGGGGAGGGGAGGGGAG+GGG + chr1_2:49233567-49233586 Msa0074570:intron 80.0%
! AGGGGAGGGGAGGGGAGGGG+AGG + chr1_2:49233570-49233589 Msa0074570:intron 80.0%
! GGGGAGGGGAGGGGAGGGGA+GGG + chr1_2:49233566-49233585 Msa0074570:intron 80.0%
! GGGGGAGGGGAGGGGAGGGG+AGG + chr1_2:49233565-49233584 Msa0074570:intron 85.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 49232582 49235963 49232582 ID=Msa0074570;Name=Msa0074570
chr1_2 mRNA 49232582 49235963 49232582 ID=Msa0074570-mRNA-1;Parent=Msa0074570;Name=Msa0074570-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|0.5|0.66|1|1|1|3|414|130
chr1_2 exon 49232582 49232708 49232582 ID=Msa0074570-mRNA-1:exon:15021;Parent=Msa0074570-mRNA-1
chr1_2 exon 49232796 49232957 49232796 ID=Msa0074570-mRNA-1:exon:15022;Parent=Msa0074570-mRNA-1
chr1_2 exon 49235373 49235963 49235373 ID=Msa0074570-mRNA-1:exon:15023;Parent=Msa0074570-mRNA-1
chr1_2 five_prime_UTR 49232582 49232654 49232582 ID=Msa0074570-mRNA-1:five_prime_utr;Parent=Msa0074570-mRNA-1
chr1_2 CDS 49232655 49232708 49232655 ID=Msa0074570-mRNA-1:cds;Parent=Msa0074570-mRNA-1
chr1_2 CDS 49232796 49232957 49232796 ID=Msa0074570-mRNA-1:cds;Parent=Msa0074570-mRNA-1
chr1_2 CDS 49235373 49235549 49235373 ID=Msa0074570-mRNA-1:cds;Parent=Msa0074570-mRNA-1
chr1_2 three_prime_UTR 49235550 49235963 49235550 ID=Msa0074570-mRNA-1:three_prime_utr;Parent=Msa0074570-mRNA-1
Gene Sequence

>Msa0074570

ATGTCTCTTCTCTTTGCTTCTTCTGTTTCTCCTCTTCAATTGTCATGCACTCAGCAGAAATGTAGGTTTTTATCACCATCATCAATTTCGCGGAGTCGAATCACCAGAACCAGAAGCATAGGTTTTAATTATTCCCTTCGAGTGGGTAATCGGAGTCGAATTACAGCGTTTTTCTTCAATCCTGCTCAAGACCCTCTCGTCAAAGAAGCTGTCAAGGAACCAGTGGCATTCTTAGGTGGGATATTTGCTGGTATTTTAAGGCTTGATTTGAATGAGGAACCTCTGAAGGAATGGGTTACGAGGACTGTTGAAGCTTCTGATATTAGTGAAGAAGAAACCAATCCAGCAGAATCAACTACTGAAGCTGCTCCACAAGAGATTGAAATTGAATAA
Protein sequence

>Msa0074570

MSLLFASSVSPLQLSCTQQKCRFLSPSSISRSRITRTRSIGFNYSLRVGNRSRITAFFFNPAQDPLVKEAVKEPVAFLGGIFAGILRLDLNEEPLKEWVTRTVEASDISEEETNPAESTTEAAPQEIEIE*