Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0088970 | sp|Q8S9L6|CRK29_ARATH | 44.201 | 638 | 322 | 10 | 38 | 669 | 40 | 649 | 0.0 | 535 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0041030 | Msa0088970 | 0.927272 | 1.481684e-91 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0088970 | MtrunA17_Chr1g0206401 | 74.656 | 655 | 138 | 7 | 19 | 673 | 18 | 644 | 0.0 | 978 |
| Msa0088970 | MtrunA17_Chr1g0206391 | 92.192 | 333 | 22 | 1 | 341 | 673 | 6 | 334 | 0.0 | 644 |
| Msa0088970 | MtrunA17_Chr1g0206461 | 48.718 | 663 | 288 | 7 | 4 | 664 | 5 | 617 | 0.0 | 622 |
| Msa0088970 | MtrunA17_Chr1g0154351 | 49.221 | 642 | 294 | 6 | 34 | 671 | 36 | 649 | 0.0 | 615 |
| Msa0088970 | MtrunA17_Chr1g0206531 | 49.168 | 661 | 286 | 8 | 7 | 664 | 8 | 621 | 0.0 | 607 |
| Msa0088970 | MtrunA17_Chr5g0426941 | 48.837 | 645 | 283 | 10 | 32 | 668 | 23 | 628 | 0.0 | 589 |
| Msa0088970 | MtrunA17_Chr1g0154311 | 47.548 | 673 | 309 | 9 | 7 | 671 | 6 | 642 | 0.0 | 588 |
| Msa0088970 | MtrunA17_Chr1g0154321 | 48.527 | 645 | 301 | 10 | 28 | 663 | 29 | 651 | 0.0 | 585 |
| Msa0088970 | MtrunA17_Chr1g0206521 | 47.605 | 668 | 299 | 10 | 8 | 674 | 654 | 1271 | 0.0 | 583 |
| Msa0088970 | MtrunA17_Chr1g0206521 | 48.323 | 656 | 288 | 10 | 20 | 674 | 24 | 629 | 0.0 | 577 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0088970 | AT4G21410.3 | 44.201 | 638 | 322 | 10 | 38 | 669 | 40 | 649 | 0.0 | 535 |
| Msa0088970 | AT4G38830.1 | 43.281 | 640 | 322 | 10 | 33 | 667 | 31 | 634 | 8.00e-175 | 514 |
| Msa0088970 | AT4G05200.1 | 42.245 | 677 | 335 | 15 | 8 | 674 | 11 | 641 | 5.30e-171 | 504 |
| Msa0088970 | AT4G23180.1 | 42.284 | 648 | 323 | 11 | 28 | 671 | 39 | 639 | 7.21e-171 | 504 |
| Msa0088970 | AT4G05200.2 | 42.245 | 677 | 335 | 15 | 8 | 674 | 29 | 659 | 1.16e-170 | 504 |
| Msa0088970 | AT4G23160.1 | 40.798 | 652 | 343 | 10 | 21 | 669 | 37 | 648 | 3.49e-170 | 503 |
| Msa0088970 | AT4G21400.1 | 42.302 | 643 | 339 | 11 | 33 | 669 | 37 | 653 | 3.96e-168 | 498 |
| Msa0088970 | AT4G21400.2 | 42.613 | 643 | 325 | 12 | 33 | 669 | 37 | 641 | 4.93e-168 | 497 |
| Msa0088970 | AT4G23140.1 | 41.789 | 615 | 306 | 9 | 66 | 669 | 67 | 640 | 1.00e-166 | 494 |
| Msa0088970 | AT4G23140.2 | 41.385 | 621 | 306 | 10 | 66 | 669 | 67 | 646 | 4.13e-164 | 487 |
Find 147 sgRNAs with CRISPR-Local
Find 381 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAAGACCCGAATATA+CGG | 0.376513 | 1_2:-70270915 | Msa0088970:CDS |
| AAAATGAGATGCATCCATGT+TGG | 0.599328 | 1_2:-70267777 | Msa0088970:CDS |
| AAACTATCATGATGCCCTTT+TGG | 0.246546 | 1_2:+70267635 | None:intergenic |
| AAATACCGAGATTCATGACG+GGG | 0.707354 | 1_2:-70268023 | Msa0088970:CDS |
| AAATGAAGATGAAGACGAAG+TGG | 0.523022 | 1_2:-70269355 | Msa0088970:CDS |
| AAATTCGGTTTCTCCTTGCT+CGG | 0.495382 | 1_2:+70269025 | None:intergenic |
| AACACATCTACTACTAGTAA+AGG | 0.489323 | 1_2:-70272383 | Msa0088970:intron |
| AACGCAGTCGCACTCTGTAG+AGG | 0.595755 | 1_2:-70273010 | Msa0088970:CDS |
| AACTTCTGCAGCACTTCTCA+TGG | 0.465160 | 1_2:-70272951 | Msa0088970:CDS |
| AAGAACGAAGTGTTGTTGAC+AGG | 0.453719 | 1_2:-70269002 | Msa0088970:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAAATAATACACACGCTG+GGG | + | chr1_2:70267467-70267486 | None:intergenic | 30.0% | |
| GAATAAAATAATACACACGC+TGG | + | chr1_2:70267469-70267488 | None:intergenic | 30.0% | |
| TCGGACAAAGAAATAAACTA+TGG | - | chr1_2:70267786-70267805 | Msa0088970:CDS | 30.0% | |
| CAATTGCTTCTTTCTCTATT+CGG | + | chr1_2:70267941-70267960 | None:intergenic | 30.0% | |
| TGAATCATTTGAATAGCGTA+AGG | + | chr1_2:70267982-70268001 | None:intergenic | 30.0% | |
| TTGTTCTGTCAAATATGACA+TGG | - | chr1_2:70268340-70268359 | Msa0088970:CDS | 30.0% | |
| TTTACTAGTAGTAGATGTGT+TGG | + | chr1_2:70268477-70268496 | None:intergenic | 30.0% | |
| AACACATCTACTACTAGTAA+AGG | - | chr1_2:70268476-70268495 | Msa0088970:CDS | 30.0% | |
| AGAGGAGAATAGTACAATAT+TGG | + | chr1_2:70268955-70268974 | None:intergenic | 30.0% | |
| GGTGATAATAAGCAAGTTAT+TGG | + | chr1_2:70268991-70269010 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 70267383 | 70273498 | 70267383 | ID=Msa0088970;Name=Msa0088970 |
| chr1_2 | mRNA | 70267383 | 70273498 | 70267383 | ID=Msa0088970-mRNA-1;Parent=Msa0088970;Name=Msa0088970-mRNA-1;_AED=0.36;_eAED=0.36;_QI=229|1|1|1|0.83|0.85|7|258|674 |
| chr1_2 | exon | 70267383 | 70267877 | 70267383 | ID=Msa0088970-mRNA-1:exon:24133;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70267997 | 70268147 | 70267997 | ID=Msa0088970-mRNA-1:exon:24132;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70268308 | 70268545 | 70268308 | ID=Msa0088970-mRNA-1:exon:24131;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70268889 | 70269099 | 70268889 | ID=Msa0088970-mRNA-1:exon:24130;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70269218 | 70269387 | 70269218 | ID=Msa0088970-mRNA-1:exon:24129;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70270903 | 70271034 | 70270903 | ID=Msa0088970-mRNA-1:exon:24128;Parent=Msa0088970-mRNA-1 |
| chr1_2 | exon | 70272384 | 70273498 | 70272384 | ID=Msa0088970-mRNA-1:exon:24127;Parent=Msa0088970-mRNA-1 |
| chr1_2 | five_prime_UTR | 70273270 | 70273498 | 70273270 | ID=Msa0088970-mRNA-1:five_prime_utr;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70272384 | 70273269 | 70272384 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70270903 | 70271034 | 70270903 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70269218 | 70269387 | 70269218 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70268889 | 70269099 | 70268889 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70268308 | 70268545 | 70268308 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70267997 | 70268147 | 70267997 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | CDS | 70267641 | 70267877 | 70267641 | ID=Msa0088970-mRNA-1:cds;Parent=Msa0088970-mRNA-1 |
| chr1_2 | three_prime_UTR | 70267383 | 70267640 | 70267383 | ID=Msa0088970-mRNA-1:three_prime_utr;Parent=Msa0088970-mRNA-1 |
| Gene Sequence |
>Msa0088970 ATGCTTTCTTCACATTGGCTTCTGTTTTCACTTCTTCCTCTGTTTTTCGCACTAAAATCCAAAGCCAACGACCAATTAATTTTCCGCTACTCCAAATGTAACGACAATCTTGGAAATTTCACCCTGGGTAGCGTCTACGAGAACAATCTTGGCACAGTTCTACAAAATATATATTCGGACAAAGAAATAAACTATGGATTCTACAATTTTTCGTACGGTGAAGAACCCGACCAAGTAAACGCAGTCGCACTCTGTAGAGGAGACGTTAATCCAGAGGATTGTCGTGATTGTCTGAAAACTTCTGCAGCACTTCTCATGGACCGGTGCCGAATAGAGAAAGAAGCAATTGGTTACTATGACTTGTGCACCTTACGCTATTCAAATGATTCAATTGTAAGTTTGATGGAAACTGAAACCGGTTATGTTTATCATCTTGTAGACAAGACAACGGTGGATGATGCGTTTAATCAAACACTTAGCAACCTGCTGGATGAACTGAAAAGCGCGGCCGCTGAAGGTGATTCGCGCCAGAAGTTTGCTGAAAAGAGTGTTAAAGTAATTGATGAAACAAGTAGTAGTAGTAGTAGTAGTAATGACACTATATATGGCCTGGTTCAATGTACACCAGATTTGTCAAAGACAGAGTGTACTGATTGTTTGGATTCGGCTTTCGGACAGATTTCATTTTGGTGCAAAGAAAGGAAAGGATGTTTGTATCTTGGACCAATTTGTTCTGTCAAATATGACATGGCTCCATTCTTTCAGTCCATAGTCAACAGTACAGAGCCGCCATCACCAGCACCACAACCTTCTCAAGCACTTCCTCCGGCAATTTCTCCTAAAACTGCACCTGCTAACTCCACCAACACATCTACTACTAGTAAAGGAAAGAGGAGGAGATTAAGAACAGCCATCGCTGTCGTTGTAGCGGTCGTTGTGACTGGCATACTGGCTGTGGGTATATGCATTTATTTAGGAAGGAAAAAACCAAGACCCGAATATACGGCTGAAATTGAAGGCCAAGAGAAAAATGAAGATGAAGACGAAGTGGAAGTGGAAGTTGAAGCATGTAATGATCTTAAAGTTGGCGAGTTAATGCAATTTGACTTTGCAAGCATCAGATTAGCTACAAACAACTTCTCTGATGCAAATAAGCTCGGTCAAGGTGGATTTGGATCTGTTTATAAGGGTACGCTCTCTGATGGACATGATATTGCTATCAAAAGGCTGGCTAACAATTCCGAGCAAGGAGAAACCGAATTTAAGAACGAAGTGTTGTTGACAGGGAAGCTTCAACATCGAAATTTAGTTAAGCTGTTAGGGTTCTGTTTACAAAGAAAAGAAAGACTACTGATATATGAATTTGTCTCCAATAAAAGTCTTGATTATATCATATTTGATCCAATGAAGCGGGCAATTTTGAATTGGGAAAGACGCTTCAAAATCATAAAAGATATTGCGCGTGGCCTTCTTTATCTTCATGAAGATTCTCGATTACAAATTGTTCACCGTGATCTCAAAACAAGCAACATTCTACTAGACGAGGAGATGAACCCAAAAATTACAGATTTTGGCCTTGCGAGATTGTTTGACGCCAATCAAACTCATGGCATGACAACAACCGTTATTGGAACATATGGATACATGGCTCCAGAGTACATTAGACATGGACAATTCTCAGTCAAGTCAGATGTGTTCAGTTTTGGCGTAATTATTTTAGAAATTGTTTGTGGCCAAAGAAATACCGAGATTCATGACGGGGAGAGTATAGAGGATCTATTAGGCATTGCATGGAAAAATTGGAAAGCAAGGACAAGTTCAGATATTGTAGATCCAATATTACACCAAGGATTTAGTAAGAATGAAAAAATGAGATGCATCCATGTTGGATTACTCTGTGTTCAAGAAGATATAGCTGCGAGGCCGAATATGAGTTCTGTTTTACTAATGCTTAATAGCACCACTTTCCCACTTCCTGAACCTTCGGAACCTCCATTTTTAATGCAACCCAAAAGGGCATCATGA |
| Protein sequence |
>Msa0088970 MLSSHWLLFSLLPLFFALKSKANDQLIFRYSKCNDNLGNFTLGSVYENNLGTVLQNIYSDKEINYGFYNFSYGEEPDQVNAVALCRGDVNPEDCRDCLKTSAALLMDRCRIEKEAIGYYDLCTLRYSNDSIVSLMETETGYVYHLVDKTTVDDAFNQTLSNLLDELKSAAAEGDSRQKFAEKSVKVIDETSSSSSSSNDTIYGLVQCTPDLSKTECTDCLDSAFGQISFWCKERKGCLYLGPICSVKYDMAPFFQSIVNSTEPPSPAPQPSQALPPAISPKTAPANSTNTSTTSKGKRRRLRTAIAVVVAVVVTGILAVGICIYLGRKKPRPEYTAEIEGQEKNEDEDEVEVEVEACNDLKVGELMQFDFASIRLATNNFSDANKLGQGGFGSVYKGTLSDGHDIAIKRLANNSEQGETEFKNEVLLTGKLQHRNLVKLLGFCLQRKERLLIYEFVSNKSLDYIIFDPMKRAILNWERRFKIIKDIARGLLYLHEDSRLQIVHRDLKTSNILLDEEMNPKITDFGLARLFDANQTHGMTTTVIGTYGYMAPEYIRHGQFSVKSDVFSFGVIILEIVCGQRNTEIHDGESIEDLLGIAWKNWKARTSSDIVDPILHQGFSKNEKMRCIHVGLLCVQEDIAARPNMSSVLLMLNSTTFPLPEPSEPPFLMQPKRAS* |
