AlfalfaGEDB | Msa0099970

M. sativa cultivar zhongmu-4 / Msa0099970

KEGG: K07198
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009987 GO:0015672 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0030001 GO:0035556 GO:0036211 GO:0042538 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0098542 GO:0140096 GO:1901564
PFAM: PF03822.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099970	XP_003588823.1	96.040	101	4	0	1	101	308	408	1.93e-56	190

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099970	sp\|Q9LEU7\|CIPK5_ARATH	55.446	101	45	0	1	101	321	421	1.32e-31	117

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099970	G7I7M2	96.040	101	4	0	1	101	308	408	9.23e-57	190

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0099970	Msa0100050	0.848472	5.996652e-60	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099970	MtrunA17_Chr1g0150321	96.040	101	4	0	1	101	308	408	1.78e-60	190
Msa0099970	MtrunA17_Chr2g0333821	54.455	101	46	0	1	101	329	429	3.95e-30	110
Msa0099970	MtrunA17_Chr4g0001331	52.000	100	48	0	1	100	334	433	2.09e-27	103
Msa0099970	MtrunA17_Chr8g0346071	46.000	100	50	2	2	98	320	418	1.36e-20	84.7
Msa0099970	MtrunA17_Chr3g0107011	46.078	102	54	1	1	101	325	426	1.58e-20	84.7
Msa0099970	MtrunA17_Chr5g0441011	47.000	100	50	2	1	98	319	417	2.23e-20	84.0
Msa0099970	MtrunA17_Chr8g0346081	46.000	100	50	2	2	98	321	419	4.14e-20	83.2
Msa0099970	MtrunA17_Chr7g0244521	46.535	101	53	1	2	101	325	425	1.05e-19	82.4
Msa0099970	MtrunA17_Chr5g0432471	44.118	102	56	1	1	101	342	443	1.28e-19	82.0
Msa0099970	MtrunA17_Chr2g0304111	48.000	100	48	2	2	98	334	432	2.00e-19	81.3
Msa0099970	MtrunA17_Chr4g0013131	45.098	102	54	1	2	101	319	420	5.14e-19	80.1
Msa0099970	MtrunA17_Chr8g0379091	46.939	98	50	2	2	99	363	458	6.30e-19	80.1
Msa0099970	MtrunA17_Chr2g0284031	45.000	100	53	2	2	101	360	457	2.47e-17	75.5
Msa0099970	MtrunA17_Chr4g0074111	48.454	97	46	2	1	94	271	366	1.47e-16	73.2
Msa0099970	MtrunA17_Chr4g0063881	33.010	103	65	2	1	100	318	419	3.79e-15	69.3
Msa0099970	MtrunA17_Chr2g0284011	37.864	103	60	3	1	100	324	425	4.81e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0099970	AT5G10930.1	55.446	101	45	0	1	101	321	421	1.34e-32	117
Msa0099970	AT5G25110.1	58.000	100	42	0	1	100	350	449	1.22e-31	115
Msa0099970	AT2G25090.1	55.446	101	45	0	1	101	331	431	4.37e-30	111
Msa0099970	AT5G58380.1	46.535	101	54	0	1	101	335	435	4.65e-23	92.0
Msa0099970	AT1G30270.3	45.098	102	52	2	2	100	245	345	3.99e-20	83.6
Msa0099970	AT1G30270.1	45.098	102	52	2	2	100	342	442	4.85e-20	83.6
Msa0099970	AT5G21326.1	43.000	100	54	2	1	98	319	417	5.82e-20	83.2
Msa0099970	AT1G30270.2	45.098	102	52	2	2	100	342	442	6.26e-20	83.2
Msa0099970	AT5G07070.1	44.118	102	56	1	1	101	322	423	8.01e-20	82.8
Msa0099970	AT2G26980.3	44.000	100	53	2	1	98	320	418	1.86e-19	82.0
Msa0099970	AT2G26980.4	44.000	100	53	2	1	98	330	428	1.94e-19	82.0
Msa0099970	AT1G29230.1	51.613	93	44	1	2	94	396	487	4.42e-18	78.2
Msa0099970	AT1G01140.2	43.137	102	54	2	2	100	328	428	7.16e-18	77.4
Msa0099970	AT1G01140.1	43.137	102	54	2	2	100	326	426	7.65e-18	77.4
Msa0099970	AT1G01140.3	44.444	99	51	2	2	97	326	423	2.05e-17	76.3
Msa0099970	AT5G45820.1	46.316	95	49	2	1	94	311	404	4.47e-17	75.1
Msa0099970	AT4G18700.1	47.312	93	47	2	2	94	350	440	1.11e-16	73.9
Msa0099970	AT5G35410.1	39.604	101	57	2	1	98	318	417	3.43e-16	72.8
Msa0099970	AT5G45810.1	47.872	94	46	3	2	94	354	445	4.28e-16	72.4
Msa0099970	AT2G34180.1	44.944	89	47	2	6	94	381	467	2.77e-15	70.1
Msa0099970	AT3G17510.2	42.718	103	54	3	1	99	246	347	8.03e-15	68.6
Msa0099970	AT3G17510.1	42.718	103	54	3	1	99	326	427	8.44e-15	68.6
Msa0099970	AT4G30960.1	46.939	98	47	3	1	94	323	419	1.84e-13	65.1

Find 24 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCGAAAGGTTTAAGGTTTC+AGG	0.157627	1_3:+6363323	Msa0099970:CDS
TCTTCGGGATTTGATCTTTC+AGG	0.176871	1_3:+6363216	Msa0099970:CDS
AGGTTAAAGATTTCAAATTA+AGG	0.271206	1_3:+6363352	Msa0099970:CDS
AAAGTGCTGCGAAAGGTTTA+AGG	0.300517	1_3:+6363316	Msa0099970:CDS
GTTTATTTCTTCGATGTCTT+CGG	0.334496	1_3:+6363200	None:intergenic
CAAGTTGTTTGAAGAAGATT+AGG	0.346453	1_3:+6363497	Msa0099970:CDS
TGAAGGGAGAAAAGGAAAAT+TGG	0.365145	1_3:+6363389	Msa0099970:CDS
TTTATTTCTTCGATGTCTTC+GGG	0.367814	1_3:+6363201	None:intergenic
TTTAAGGTTTCAGGTTAAGA+AGG	0.409931	1_3:+6363332	Msa0099970:CDS
AGGTTGATTGAAGGGAGAAA+AGG	0.475798	1_3:+6363381	Msa0099970:CDS
AATTCAACCACCGCAAGCTC+CGG	0.484594	1_3:-6363441	None:intergenic
GGTTACAAAGGTTGATTGAA+GGG	0.487017	1_3:+6363373	Msa0099970:CDS
AGGTTACAAAGGTTGATTGA+AGG	0.503469	1_3:+6363372	Msa0099970:CDS
AAAAGGAAAATTGGCGGTGA+CGG	0.521587	1_3:+6363398	Msa0099970:CDS
GACATATTCAAAAGTATCAC+CGG	0.539078	1_3:-6363475	None:intergenic
CGGCTCCACCTCGTATATCT+CGG	0.545722	1_3:-6363421	None:intergenic
CGAGATATACGAGGTGGAGC+CGG	0.549592	1_3:+6363422	Msa0099970:CDS
CGAGGTGGAGCCGGAGCTTG+CGG	0.556902	1_3:+6363431	Msa0099970:CDS
ATTTCAAATTAAGGTTACAA+AGG	0.564420	1_3:+6363361	Msa0099970:CDS
AGGGAGAAAAGGAAAATTGG+CGG	0.574181	1_3:+6363392	Msa0099970:CDS
AAAATTGAAAGTGCTGCGAA+AGG	0.580016	1_3:+6363309	Msa0099970:CDS
GGTGGAGCCGGAGCTTGCGG+TGG	0.623355	1_3:+6363434	Msa0099970:CDS
GACGGCCGAGATATACGAGG+TGG	0.691896	1_3:+6363416	Msa0099970:CDS
GGTGACGGCCGAGATATACG+AGG	0.772567	1_3:+6363413	Msa0099970:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAAGAAAAGAAAAAAAG+AGG	+	chr1_3:6363243-6363262	Msa0099970:CDS	20.0%
!!	AGGTTAAAGATTTCAAATTA+AGG	+	chr1_3:6363352-6363371	Msa0099970:CDS	20.0%
!!	ATTTCAAATTAAGGTTACAA+AGG	+	chr1_3:6363361-6363380	Msa0099970:CDS	20.0%
!	AGAAAAGAAAAAAAGAGGTT+CGG	+	chr1_3:6363248-6363267	Msa0099970:CDS	25.0%
!!	GTTGAATTTTCTAAATGTTC+CGG	+	chr1_3:6363456-6363475	Msa0099970:CDS	25.0%
!!	TTTAAGGTTTCAGGTTAAGA+AGG	+	chr1_3:6363332-6363351	Msa0099970:CDS	30.0%
!!	GACATATTCAAAAGTATCAC+CGG	-	chr1_3:6363478-6363497	None:intergenic	30.0%
!!	AAAATTGAAAGTGCTGCGAA+AGG	+	chr1_3:6363309-6363328	Msa0099970:CDS	35.0%
!	AGGTTACAAAGGTTGATTGA+AGG	+	chr1_3:6363372-6363391	Msa0099970:CDS	35.0%
!	GGTTACAAAGGTTGATTGAA+GGG	+	chr1_3:6363373-6363392	Msa0099970:CDS	35.0%
	TGAAGGGAGAAAAGGAAAAT+TGG	+	chr1_3:6363389-6363408	Msa0099970:CDS	35.0%
	TCTTCGGGATTTGATCTTTC+AGG	+	chr1_3:6363216-6363235	Msa0099970:CDS	40.0%
!!	AAAGTGCTGCGAAAGGTTTA+AGG	+	chr1_3:6363316-6363335	Msa0099970:CDS	40.0%
!	TGCGAAAGGTTTAAGGTTTC+AGG	+	chr1_3:6363323-6363342	Msa0099970:CDS	40.0%
!	AGGTTGATTGAAGGGAGAAA+AGG	+	chr1_3:6363381-6363400	Msa0099970:CDS	40.0%
	AGGGAGAAAAGGAAAATTGG+CGG	+	chr1_3:6363392-6363411	Msa0099970:CDS	40.0%
	AAAAGGAAAATTGGCGGTGA+CGG	+	chr1_3:6363398-6363417	Msa0099970:CDS	40.0%
	AATTCAACCACCGCAAGCTC+CGG	-	chr1_3:6363444-6363463	None:intergenic	50.0%
	CGGCTCCACCTCGTATATCT+CGG	-	chr1_3:6363424-6363443	None:intergenic	55.0%
	CGAGATATACGAGGTGGAGC+CGG	+	chr1_3:6363422-6363441	Msa0099970:CDS	55.0%
!	GGTGACGGCCGAGATATACG+AGG	+	chr1_3:6363413-6363432	Msa0099970:CDS	60.0%
	GACGGCCGAGATATACGAGG+TGG	+	chr1_3:6363416-6363435	Msa0099970:CDS	60.0%
	CGAGGTGGAGCCGGAGCTTG+CGG	+	chr1_3:6363431-6363450	Msa0099970:CDS	70.0%
	GGTGGAGCCGGAGCTTGCGG+TGG	+	chr1_3:6363434-6363453	Msa0099970:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	6363213	6363518	6363213	ID=Msa0099970;Name=Msa0099970
chr1_3	mRNA	6363213	6363518	6363213	ID=Msa0099970-mRNA-1;Parent=Msa0099970;Name=Msa0099970-mRNA-1;_AED=0.38;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|101
chr1_3	exon	6363213	6363518	6363213	ID=Msa0099970-mRNA-1:exon:2392;Parent=Msa0099970-mRNA-1
chr1_3	CDS	6363213	6363518	6363213	ID=Msa0099970-mRNA-1:cds;Parent=Msa0099970-mRNA-1

Gene Sequence	>Msa0099970 ATGTCTTCGGGATTTGATCTTTCAGGTTTGTTTGAAGAAAAGAAAAAAAGAGGTTCGGTTTTCACTTCAAAGTGTTCTGTCTCAGAAATTGTAACAAAAATTGAAAGTGCTGCGAAAGGTTTAAGGTTTCAGGTTAAGAAGGTTAAAGATTTCAAATTAAGGTTACAAAGGTTGATTGAAGGGAGAAAAGGAAAATTGGCGGTGACGGCCGAGATATACGAGGTGGAGCCGGAGCTTGCGGTGGTTGAATTTTCTAAATGTTCCGGTGATACTTTTGAATATGTCAAGTTGTTTGAAGAAGATTAG
Protein sequence	>Msa0099970 MSSGFDLSGLFEEKKKRGSVFTSKCSVSEIVTKIESAAKGLRFQVKKVKDFKLRLQRLIEGRKGKLAVTAEIYEVEPELAVVEFSKCSGDTFEYVKLFEED*