AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0104400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104400 MtrunA17_Chr1g0158661 74.552 279 57 5 1 272 1 272 6.02e-139 407
Msa0104400 MtrunA17_Chr1g0158631 73.529 272 52 4 1 258 1 266 8.50e-132 389
Msa0104400 MtrunA17_Chr1g0157791 38.909 275 124 9 13 259 3 261 7.81e-45 160
Msa0104400 MtrunA17_Chr1g0157881 37.500 272 117 10 37 257 58 327 2.11e-44 160
Msa0104400 MtrunA17_Chr1g0158061 40.574 244 104 8 37 257 33 258 4.11e-44 151
Msa0104400 MtrunA17_Chr1g0157911 43.038 237 111 8 37 257 40 268 1.33e-43 157
Msa0104400 MtrunA17_Chr1g0158731 41.818 220 109 6 37 250 41 247 1.54e-42 147
Msa0104400 MtrunA17_Chr1g0158411 37.175 269 130 8 16 261 23 275 1.61e-42 154
Msa0104400 MtrunA17_Chr3g0088331 40.000 235 117 10 37 260 44 265 1.87e-41 144
Msa0104400 MtrunA17_Chr1g0158041 41.202 233 101 6 30 241 26 243 2.22e-41 151
Msa0104400 MtrunA17_Chr1g0157861 35.793 271 129 8 28 257 36 302 1.68e-40 149
Msa0104400 MtrunA17_Chr1g0157961 42.986 221 97 5 37 242 41 247 2.11e-40 142
Msa0104400 MtrunA17_Chr1g0158191 39.676 247 109 8 16 236 4 236 4.80e-40 147
Msa0104400 MtrunA17_Chr1g0158151 39.922 258 116 9 37 271 34 275 1.53e-39 145
Msa0104400 MtrunA17_Chr1g0157991 42.667 225 96 6 37 242 36 246 2.21e-39 145
Msa0104400 MtrunA17_Chr6g0467711 37.838 259 129 10 23 260 22 269 6.20e-39 144
Msa0104400 MtrunA17_Chr1g0158461 34.828 290 139 8 13 262 19 298 7.52e-38 135
Msa0104400 MtrunA17_Chr1g0158331 37.247 247 117 9 37 257 51 285 3.72e-37 134
Msa0104400 MtrunA17_Chr1g0158761 35.498 231 121 6 16 241 9 216 4.61e-36 135
Msa0104400 MtrunA17_Chr1g0158121 64.634 82 29 0 37 118 37 118 1.28e-30 111
Msa0104400 MtrunA17_Chr1g0158571 61.165 103 32 3 159 261 2 96 3.22e-30 118
Msa0104400 MtrunA17_Chr1g0158541 31.227 269 143 11 16 257 17 270 3.71e-29 116
Msa0104400 MtrunA17_Chr1g0158301 31.008 258 154 10 16 257 19 268 3.98e-28 113
Msa0104400 MtrunA17_Chr1g0160761 33.878 245 137 10 17 247 7 240 1.47e-25 105
Msa0104400 MtrunA17_Chr1g0160701 33.636 220 118 9 35 241 44 248 1.11e-21 94.4
Msa0104400 MtrunA17_Chr1g0160561 33.333 228 122 9 31 241 29 243 5.02e-21 92.4
Msa0104400 MtrunA17_Chr1g0160661 28.571 273 163 8 1 263 21 271 7.18e-21 91.7
Msa0104400 MtrunA17_Chr1g0160721 29.183 257 147 9 16 259 18 252 8.19e-20 89.0
Msa0104400 MtrunA17_Chr1g0160711 30.435 230 120 10 31 241 22 230 1.38e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCACAACTCTTGCATATC+TGG 0.189605 1_3:-12279570 None:intergenic
CAATTGGTGGGTTGAATGTT+TGG 0.225192 1_3:-12279830 None:intergenic
TTGTTACTCTCACATCCTTC+TGG 0.271931 1_3:+12280113 Msa0104400:CDS
GCTATGGATTTGAAATTTCC+TGG 0.305840 1_3:+12280799 Msa0104400:CDS
ACTTACCACGATAACATTAT+AGG 0.309676 1_3:+12280166 Msa0104400:CDS
GTGAGGGAGAGAGGAGCAAT+TGG 0.327106 1_3:-12279846 None:intergenic
ATGGTTTCAACCTCTAACTT+TGG 0.331944 1_3:+12279539 Msa0104400:CDS
CGACCCACAACTTGGAATAT+TGG 0.341311 1_3:+12280069 Msa0104400:CDS
GTACACTCCATGTAAATTAC+AGG 0.365991 1_3:-12279962 None:intergenic
TGTTACTCTCACATCCTTCT+GGG 0.367632 1_3:+12280114 Msa0104400:CDS
GTACTGTTACCACAGTGGTC+TGG 0.396707 1_3:-12279701 None:intergenic
TCTGAATGGATTAGTTATGT+TGG 0.397719 1_3:-12279666 None:intergenic
TAAGAAGAAGTGAGGGAGAG+AGG 0.410699 1_3:-12279855 None:intergenic
AATTGGTGGGTTGAATGTTT+GGG 0.419800 1_3:-12279829 None:intergenic
CACAACATATATGTAATTGT+TGG 0.438423 1_3:+12280046 Msa0104400:CDS
CAACCTTGTTCTTCTTATTG+TGG 0.455000 1_3:+12279635 Msa0104400:CDS
TACTGTTACCACAGTGGTCT+GGG 0.470002 1_3:-12279700 None:intergenic
AGGGAGAGAGGAGCAATTGG+TGG 0.474420 1_3:-12279843 None:intergenic
GGCTCCAATATTCCAAGTTG+TGG 0.482055 1_3:-12280073 None:intergenic
ACAGTATTGAAGTTGTATGA+TGG 0.489285 1_3:+12279752 Msa0104400:CDS
ATTCAGAAGGCACTAGGCTA+TGG 0.492318 1_3:+12280783 Msa0104400:CDS
TACTTATCCACAGCGTTACT+AGG 0.493264 1_3:-12279992 None:intergenic
ATCTATGCCTGTAATTTACA+TGG 0.494744 1_3:+12279955 Msa0104400:CDS
ATTGTTGGCGACCCACAACT+TGG 0.495087 1_3:+12280061 Msa0104400:CDS
ATCACAACTCTTGCATATCT+GGG 0.496177 1_3:-12279569 None:intergenic
GCAATGATCACAATGACAAA+AGG 0.499670 1_3:-12280829 None:intergenic
GGGAGCATATACTTACTTCT+CGG 0.500725 1_3:+12280135 Msa0104400:CDS
ATGACAAAAGGCTTGCATCC+AGG 0.510377 1_3:-12280817 None:intergenic
AACAAAGGACTACGGTCTTA+CGG 0.517449 1_3:-12280899 None:intergenic
CAATAAGAAGAACAAGGTTG+TGG 0.523362 1_3:-12279632 None:intergenic
TGCTACCTCAAGGAGCCTAC+TGG 0.524076 1_3:+12280849 Msa0104400:CDS
CGGTCTTACGGTAGTCGTCT+CGG 0.530168 1_3:-12280887 None:intergenic
TTGCTGTATAAGAAGAAGTG+AGG 0.550159 1_3:-12279863 None:intergenic
TACGAATACAGCTTCATGCA+TGG 0.551598 1_3:+12280018 Msa0104400:CDS
GCTCCAATATTCCAAGTTGT+GGG 0.559264 1_3:-12280072 None:intergenic
GGAGCATATACTTACTTCTC+GGG 0.566052 1_3:+12280136 Msa0104400:CDS
GGGAGAGAGGAGCAATTGGT+GGG 0.574563 1_3:-12279842 None:intergenic
TGCTGTATAAGAAGAAGTGA+GGG 0.581666 1_3:-12279862 None:intergenic
ACGAATACAGCTTCATGCAT+GGG 0.594756 1_3:+12280019 Msa0104400:CDS
TTGTGATCATTGCTACCTCA+AGG 0.597147 1_3:+12280839 Msa0104400:CDS
GTTACTCTCACATCCTTCTG+GGG 0.604732 1_3:+12280115 Msa0104400:CDS
GAAAATGTAACAAAGGACTA+CGG 0.611951 1_3:-12280907 None:intergenic
ATCATACATCGATATTCAGA+AGG 0.615231 1_3:+12280770 Msa0104400:CDS
TGGTAACAGTACGTACGAGT+TGG 0.621121 1_3:+12279712 Msa0104400:CDS
ATCGATATTCAGAAGGCACT+AGG 0.625875 1_3:+12280777 Msa0104400:CDS
ACGTAATGAAAATGTAACAA+AGG 0.628114 1_3:-12280914 None:intergenic
TTGTACCTATAATGTTATCG+TGG 0.635997 1_3:-12280171 None:intergenic
TTTCCACAATAAGAAGAACA+AGG 0.637511 1_3:-12279638 None:intergenic
GTAAGTATATGCTCCCCAGA+AGG 0.638335 1_3:-12280128 None:intergenic
TAACACTGAATCTCGCCAGT+AGG 0.643394 1_3:-12280864 None:intergenic
TCTCGCCAGTAGGCTCCTTG+AGG 0.649135 1_3:-12280854 None:intergenic
TTATCCACAGCGTTACTAGG+TGG 0.678496 1_3:-12279989 None:intergenic
ACAAGCTTAACACGGCATTG+AGG 0.681882 1_3:-12280094 None:intergenic
AAGTCCACCTAGTAACGCTG+TGG 0.692399 1_3:+12279985 Msa0104400:CDS
TGAGAGTAACAAGCTTAACA+CGG 0.706562 1_3:-12280102 None:intergenic
AGGCATAGATAACTCAGACG+TGG 0.709629 1_3:-12279942 None:intergenic
CGTACGTACTGTTACCACAG+TGG 0.756220 1_3:-12279706 None:intergenic
AAACATGACCCAGACCACTG+TGG 0.761857 1_3:+12279692 Msa0104400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTGTTTTTTTTTTTCA+TGG - chr1_3:12280401-12280420 None:intergenic 15.0%
!!! CAATTTGTTTTTTTTTTTCA+TGG - chr1_3:12280401-12280420 None:intergenic 15.0%
!! TCTATTCAAGTGTAAATAAA+TGG - chr1_3:12280575-12280594 None:intergenic 20.0%
!! TTTATTTACACTTGAATAGA+AGG + chr1_3:12280575-12280594 Msa0104400:intron 20.0%
!! TATAGACAAAAAAATCTACA+CGG - chr1_3:12280724-12280743 None:intergenic 20.0%
!! TCTATTCAAGTGTAAATAAA+TGG - chr1_3:12280575-12280594 None:intergenic 20.0%
!! TTTATTTACACTTGAATAGA+AGG + chr1_3:12280575-12280594 Msa0104400:intron 20.0%
!! TATAGACAAAAAAATCTACA+CGG - chr1_3:12280724-12280743 None:intergenic 20.0%
!!! GTATGAATTAGCTTCTTTTT+GGG - chr1_3:12279897-12279916 None:intergenic 25.0%
! CACAACATATATGTAATTGT+TGG + chr1_3:12280046-12280065 Msa0104400:CDS 25.0%
! ATATAGTCAAATTGAGTCAT+TGG - chr1_3:12280222-12280241 None:intergenic 25.0%
!!! ATTGATTTTGTCATCCAAAA+AGG - chr1_3:12280629-12280648 None:intergenic 25.0%
! ACGTAATGAAAATGTAACAA+AGG - chr1_3:12280917-12280936 None:intergenic 25.0%
!!! GTATGAATTAGCTTCTTTTT+GGG - chr1_3:12279897-12279916 None:intergenic 25.0%
! CACAACATATATGTAATTGT+TGG + chr1_3:12280046-12280065 Msa0104400:CDS 25.0%
! ATATAGTCAAATTGAGTCAT+TGG - chr1_3:12280222-12280241 None:intergenic 25.0%
!!! ATTGATTTTGTCATCCAAAA+AGG - chr1_3:12280629-12280648 None:intergenic 25.0%
! ACGTAATGAAAATGTAACAA+AGG - chr1_3:12280917-12280936 None:intergenic 25.0%
TTTCCACAATAAGAAGAACA+AGG - chr1_3:12279641-12279660 None:intergenic 30.0%
!! TCTGAATGGATTAGTTATGT+TGG - chr1_3:12279669-12279688 None:intergenic 30.0%
! ACAGTATTGAAGTTGTATGA+TGG + chr1_3:12279752-12279771 Msa0104400:CDS 30.0%
!! CGTATGAATTAGCTTCTTTT+TGG - chr1_3:12279898-12279917 None:intergenic 30.0%
ATCTATGCCTGTAATTTACA+TGG + chr1_3:12279955-12279974 Msa0104400:CDS 30.0%
ACTTACCACGATAACATTAT+AGG + chr1_3:12280166-12280185 Msa0104400:CDS 30.0%
TTGTACCTATAATGTTATCG+TGG - chr1_3:12280174-12280193 None:intergenic 30.0%
! GAGTCATTGGTTATATAAGA+AGG - chr1_3:12280209-12280228 None:intergenic 30.0%
ATCATACATCGATATTCAGA+AGG + chr1_3:12280770-12280789 Msa0104400:CDS 30.0%
GAAAATGTAACAAAGGACTA+CGG - chr1_3:12280910-12280929 None:intergenic 30.0%
TTTCCACAATAAGAAGAACA+AGG - chr1_3:12279641-12279660 None:intergenic 30.0%
!! TCTGAATGGATTAGTTATGT+TGG - chr1_3:12279669-12279688 None:intergenic 30.0%
! ACAGTATTGAAGTTGTATGA+TGG + chr1_3:12279752-12279771 Msa0104400:CDS 30.0%
!! CGTATGAATTAGCTTCTTTT+TGG - chr1_3:12279898-12279917 None:intergenic 30.0%
ATCTATGCCTGTAATTTACA+TGG + chr1_3:12279955-12279974 Msa0104400:CDS 30.0%
ACTTACCACGATAACATTAT+AGG + chr1_3:12280166-12280185 Msa0104400:CDS 30.0%
TTGTACCTATAATGTTATCG+TGG - chr1_3:12280174-12280193 None:intergenic 30.0%
! GAGTCATTGGTTATATAAGA+AGG - chr1_3:12280209-12280228 None:intergenic 30.0%
ATCATACATCGATATTCAGA+AGG + chr1_3:12280770-12280789 Msa0104400:CDS 30.0%
GAAAATGTAACAAAGGACTA+CGG - chr1_3:12280910-12280929 None:intergenic 30.0%
ATGGTTTCAACCTCTAACTT+TGG + chr1_3:12279539-12279558 Msa0104400:CDS 35.0%
ATCACAACTCTTGCATATCT+GGG - chr1_3:12279572-12279591 None:intergenic 35.0%
TATCACAACTCTTGCATATC+TGG - chr1_3:12279573-12279592 None:intergenic 35.0%
CAATAAGAAGAACAAGGTTG+TGG - chr1_3:12279635-12279654 None:intergenic 35.0%
CAACCTTGTTCTTCTTATTG+TGG + chr1_3:12279635-12279654 Msa0104400:CDS 35.0%
!!! GGGTCATGTTTTAATCTGAA+TGG - chr1_3:12279683-12279702 None:intergenic 35.0%
!! AATTGGTGGGTTGAATGTTT+GGG - chr1_3:12279832-12279851 None:intergenic 35.0%
TGCTGTATAAGAAGAAGTGA+GGG - chr1_3:12279865-12279884 None:intergenic 35.0%
TTGCTGTATAAGAAGAAGTG+AGG - chr1_3:12279866-12279885 None:intergenic 35.0%
GTACACTCCATGTAAATTAC+AGG - chr1_3:12279965-12279984 None:intergenic 35.0%
TGAGAGTAACAAGCTTAACA+CGG - chr1_3:12280105-12280124 None:intergenic 35.0%
ATTGAATATCTTACGACCGA+TGG + chr1_3:12280417-12280436 Msa0104400:intron 35.0%
TCATAGTTGTCCTATTCCAT+CGG - chr1_3:12280436-12280455 None:intergenic 35.0%
GGAATAGGACAACTATGATA+TGG + chr1_3:12280438-12280457 Msa0104400:intron 35.0%
ACACTTGAATAGAAGGTAAG+CGG + chr1_3:12280582-12280601 Msa0104400:intron 35.0%
! GCTATGGATTTGAAATTTCC+TGG + chr1_3:12280799-12280818 Msa0104400:CDS 35.0%
GCAATGATCACAATGACAAA+AGG - chr1_3:12280832-12280851 None:intergenic 35.0%
ATGGTTTCAACCTCTAACTT+TGG + chr1_3:12279539-12279558 Msa0104400:CDS 35.0%
ATCACAACTCTTGCATATCT+GGG - chr1_3:12279572-12279591 None:intergenic 35.0%
TATCACAACTCTTGCATATC+TGG - chr1_3:12279573-12279592 None:intergenic 35.0%
CAATAAGAAGAACAAGGTTG+TGG - chr1_3:12279635-12279654 None:intergenic 35.0%
CAACCTTGTTCTTCTTATTG+TGG + chr1_3:12279635-12279654 Msa0104400:CDS 35.0%
!!! GGGTCATGTTTTAATCTGAA+TGG - chr1_3:12279683-12279702 None:intergenic 35.0%
!! AATTGGTGGGTTGAATGTTT+GGG - chr1_3:12279832-12279851 None:intergenic 35.0%
TGCTGTATAAGAAGAAGTGA+GGG - chr1_3:12279865-12279884 None:intergenic 35.0%
TTGCTGTATAAGAAGAAGTG+AGG - chr1_3:12279866-12279885 None:intergenic 35.0%
GTACACTCCATGTAAATTAC+AGG - chr1_3:12279965-12279984 None:intergenic 35.0%
TGAGAGTAACAAGCTTAACA+CGG - chr1_3:12280105-12280124 None:intergenic 35.0%
ATTGAATATCTTACGACCGA+TGG + chr1_3:12280417-12280436 Msa0104400:intron 35.0%
TCATAGTTGTCCTATTCCAT+CGG - chr1_3:12280436-12280455 None:intergenic 35.0%
GGAATAGGACAACTATGATA+TGG + chr1_3:12280438-12280457 Msa0104400:intron 35.0%
ACACTTGAATAGAAGGTAAG+CGG + chr1_3:12280582-12280601 Msa0104400:intron 35.0%
! GCTATGGATTTGAAATTTCC+TGG + chr1_3:12280799-12280818 Msa0104400:CDS 35.0%
GCAATGATCACAATGACAAA+AGG - chr1_3:12280832-12280851 None:intergenic 35.0%
! GGGTTTTTCTCCAAAGTTAG+AGG - chr1_3:12279552-12279571 None:intergenic 40.0%
!! GGTTGTGGATAAGTTTTGTG+TGG - chr1_3:12279620-12279639 None:intergenic 40.0%
!! CAATTGGTGGGTTGAATGTT+TGG - chr1_3:12279833-12279852 None:intergenic 40.0%
TACTTATCCACAGCGTTACT+AGG - chr1_3:12279995-12280014 None:intergenic 40.0%
TACGAATACAGCTTCATGCA+TGG + chr1_3:12280018-12280037 Msa0104400:CDS 40.0%
ACGAATACAGCTTCATGCAT+GGG + chr1_3:12280019-12280038 Msa0104400:CDS 40.0%
GCTCCAATATTCCAAGTTGT+GGG - chr1_3:12280075-12280094 None:intergenic 40.0%
TTGTTACTCTCACATCCTTC+TGG + chr1_3:12280113-12280132 Msa0104400:CDS 40.0%
TGTTACTCTCACATCCTTCT+GGG + chr1_3:12280114-12280133 Msa0104400:CDS 40.0%
! GGGAGCATATACTTACTTCT+CGG + chr1_3:12280135-12280154 Msa0104400:CDS 40.0%
GGAGCATATACTTACTTCTC+GGG + chr1_3:12280136-12280155 Msa0104400:CDS 40.0%
TATCTTACGACCGATGGAAT+AGG + chr1_3:12280423-12280442 Msa0104400:intron 40.0%
! ACTGTACCACTAGACCTTTT+TGG + chr1_3:12280612-12280631 Msa0104400:intron 40.0%
TGTCATCCAAAAAGGTCTAG+TGG - chr1_3:12280621-12280640 None:intergenic 40.0%
!! ATCGATATTCAGAAGGCACT+AGG + chr1_3:12280777-12280796 Msa0104400:CDS 40.0%
TTGTGATCATTGCTACCTCA+AGG + chr1_3:12280839-12280858 Msa0104400:CDS 40.0%
AACAAAGGACTACGGTCTTA+CGG - chr1_3:12280902-12280921 None:intergenic 40.0%
! GGGTTTTTCTCCAAAGTTAG+AGG - chr1_3:12279552-12279571 None:intergenic 40.0%
!! GGTTGTGGATAAGTTTTGTG+TGG - chr1_3:12279620-12279639 None:intergenic 40.0%
!! CAATTGGTGGGTTGAATGTT+TGG - chr1_3:12279833-12279852 None:intergenic 40.0%
TACTTATCCACAGCGTTACT+AGG - chr1_3:12279995-12280014 None:intergenic 40.0%
TACGAATACAGCTTCATGCA+TGG + chr1_3:12280018-12280037 Msa0104400:CDS 40.0%
ACGAATACAGCTTCATGCAT+GGG + chr1_3:12280019-12280038 Msa0104400:CDS 40.0%
GCTCCAATATTCCAAGTTGT+GGG - chr1_3:12280075-12280094 None:intergenic 40.0%
TTGTTACTCTCACATCCTTC+TGG + chr1_3:12280113-12280132 Msa0104400:CDS 40.0%
TGTTACTCTCACATCCTTCT+GGG + chr1_3:12280114-12280133 Msa0104400:CDS 40.0%
! GGGAGCATATACTTACTTCT+CGG + chr1_3:12280135-12280154 Msa0104400:CDS 40.0%
GGAGCATATACTTACTTCTC+GGG + chr1_3:12280136-12280155 Msa0104400:CDS 40.0%
TATCTTACGACCGATGGAAT+AGG + chr1_3:12280423-12280442 Msa0104400:intron 40.0%
! ACTGTACCACTAGACCTTTT+TGG + chr1_3:12280612-12280631 Msa0104400:intron 40.0%
TGTCATCCAAAAAGGTCTAG+TGG - chr1_3:12280621-12280640 None:intergenic 40.0%
!! ATCGATATTCAGAAGGCACT+AGG + chr1_3:12280777-12280796 Msa0104400:CDS 40.0%
TTGTGATCATTGCTACCTCA+AGG + chr1_3:12280839-12280858 Msa0104400:CDS 40.0%
AACAAAGGACTACGGTCTTA+CGG - chr1_3:12280902-12280921 None:intergenic 40.0%
TACTGTTACCACAGTGGTCT+GGG - chr1_3:12279703-12279722 None:intergenic 45.0%
TGGTAACAGTACGTACGAGT+TGG + chr1_3:12279712-12279731 Msa0104400:CDS 45.0%
TAAGAAGAAGTGAGGGAGAG+AGG - chr1_3:12279858-12279877 None:intergenic 45.0%
AGGCATAGATAACTCAGACG+TGG - chr1_3:12279945-12279964 None:intergenic 45.0%
TTATCCACAGCGTTACTAGG+TGG - chr1_3:12279992-12280011 None:intergenic 45.0%
CGACCCACAACTTGGAATAT+TGG + chr1_3:12280069-12280088 Msa0104400:CDS 45.0%
GGCTCCAATATTCCAAGTTG+TGG - chr1_3:12280076-12280095 None:intergenic 45.0%
ACAAGCTTAACACGGCATTG+AGG - chr1_3:12280097-12280116 None:intergenic 45.0%
GTTACTCTCACATCCTTCTG+GGG + chr1_3:12280115-12280134 Msa0104400:CDS 45.0%
GTAAGTATATGCTCCCCAGA+AGG - chr1_3:12280131-12280150 None:intergenic 45.0%
!! ATTCAGAAGGCACTAGGCTA+TGG + chr1_3:12280783-12280802 Msa0104400:CDS 45.0%
ATGACAAAAGGCTTGCATCC+AGG - chr1_3:12280820-12280839 None:intergenic 45.0%
TAACACTGAATCTCGCCAGT+AGG - chr1_3:12280867-12280886 None:intergenic 45.0%
TACTGTTACCACAGTGGTCT+GGG - chr1_3:12279703-12279722 None:intergenic 45.0%
TGGTAACAGTACGTACGAGT+TGG + chr1_3:12279712-12279731 Msa0104400:CDS 45.0%
TAAGAAGAAGTGAGGGAGAG+AGG - chr1_3:12279858-12279877 None:intergenic 45.0%
AGGCATAGATAACTCAGACG+TGG - chr1_3:12279945-12279964 None:intergenic 45.0%
TTATCCACAGCGTTACTAGG+TGG - chr1_3:12279992-12280011 None:intergenic 45.0%
CGACCCACAACTTGGAATAT+TGG + chr1_3:12280069-12280088 Msa0104400:CDS 45.0%
GGCTCCAATATTCCAAGTTG+TGG - chr1_3:12280076-12280095 None:intergenic 45.0%
ACAAGCTTAACACGGCATTG+AGG - chr1_3:12280097-12280116 None:intergenic 45.0%
GTTACTCTCACATCCTTCTG+GGG + chr1_3:12280115-12280134 Msa0104400:CDS 45.0%
GTAAGTATATGCTCCCCAGA+AGG - chr1_3:12280131-12280150 None:intergenic 45.0%
!! ATTCAGAAGGCACTAGGCTA+TGG + chr1_3:12280783-12280802 Msa0104400:CDS 45.0%
ATGACAAAAGGCTTGCATCC+AGG - chr1_3:12280820-12280839 None:intergenic 45.0%
TAACACTGAATCTCGCCAGT+AGG - chr1_3:12280867-12280886 None:intergenic 45.0%
!!! ATAAATATTATTTCTTTTAT+AGG + chr1_3:12280741-12280760 Msa0104400:intron 5.0%
!!! ATAAATATTATTTCTTTTAT+AGG + chr1_3:12280741-12280760 Msa0104400:intron 5.0%
AAACATGACCCAGACCACTG+TGG + chr1_3:12279692-12279711 Msa0104400:CDS 50.0%
GTACTGTTACCACAGTGGTC+TGG - chr1_3:12279704-12279723 None:intergenic 50.0%
CGTACGTACTGTTACCACAG+TGG - chr1_3:12279709-12279728 None:intergenic 50.0%
AAGTCCACCTAGTAACGCTG+TGG + chr1_3:12279985-12280004 Msa0104400:CDS 50.0%
ATTGTTGGCGACCCACAACT+TGG + chr1_3:12280061-12280080 Msa0104400:CDS 50.0%
AAACATGACCCAGACCACTG+TGG + chr1_3:12279692-12279711 Msa0104400:CDS 50.0%
GTACTGTTACCACAGTGGTC+TGG - chr1_3:12279704-12279723 None:intergenic 50.0%
CGTACGTACTGTTACCACAG+TGG - chr1_3:12279709-12279728 None:intergenic 50.0%
AAGTCCACCTAGTAACGCTG+TGG + chr1_3:12279985-12280004 Msa0104400:CDS 50.0%
ATTGTTGGCGACCCACAACT+TGG + chr1_3:12280061-12280080 Msa0104400:CDS 50.0%
GGGAGAGAGGAGCAATTGGT+GGG - chr1_3:12279845-12279864 None:intergenic 55.0%
AGGGAGAGAGGAGCAATTGG+TGG - chr1_3:12279846-12279865 None:intergenic 55.0%
GTGAGGGAGAGAGGAGCAAT+TGG - chr1_3:12279849-12279868 None:intergenic 55.0%
TGCTACCTCAAGGAGCCTAC+TGG + chr1_3:12280849-12280868 Msa0104400:CDS 55.0%
CGGTCTTACGGTAGTCGTCT+CGG - chr1_3:12280890-12280909 None:intergenic 55.0%
GGGAGAGAGGAGCAATTGGT+GGG - chr1_3:12279845-12279864 None:intergenic 55.0%
AGGGAGAGAGGAGCAATTGG+TGG - chr1_3:12279846-12279865 None:intergenic 55.0%
GTGAGGGAGAGAGGAGCAAT+TGG - chr1_3:12279849-12279868 None:intergenic 55.0%
TGCTACCTCAAGGAGCCTAC+TGG + chr1_3:12280849-12280868 Msa0104400:CDS 55.0%
CGGTCTTACGGTAGTCGTCT+CGG - chr1_3:12280890-12280909 None:intergenic 55.0%
TCTCGCCAGTAGGCTCCTTG+AGG - chr1_3:12280857-12280876 None:intergenic 60.0%
TCTCGCCAGTAGGCTCCTTG+AGG - chr1_3:12280857-12280876 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 12279539 12280938 12279539 ID=Msa0104400;Name=Msa0104400
chr1_3 mRNA 12279539 12280938 12279539 ID=Msa0104400-mRNA-1;Parent=Msa0104400;Name=Msa0104400-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|1|0|1|1|1|2|0|274
chr1_3 exon 12279539 12280187 12279539 ID=Msa0104400-mRNA-1:exon:4881;Parent=Msa0104400-mRNA-1
chr1_3 exon 12280763 12280938 12280763 ID=Msa0104400-mRNA-1:exon:4882;Parent=Msa0104400-mRNA-1
chr1_3 CDS 12279539 12280187 12279539 ID=Msa0104400-mRNA-1:cds;Parent=Msa0104400-mRNA-1
chr1_3 CDS 12280763 12280938 12280763 ID=Msa0104400-mRNA-1:cds;Parent=Msa0104400-mRNA-1
Gene Sequence

>Msa0104400

ATGGTTTCAACCTCTAACTTTGGAGAAAAACCCAGATATGCAAGAGTTGTGATAGTGTTTCTTTACTTGTTTCTACTTCCACACAAAACTTATCCACAACCTTGTTCTTCTTATTGTGGAAAAATTTCCAACATAACTAATCCATTCAGATTAAAACATGACCCAGACCACTGTGGTAACAGTACGTACGAGTTGGATTGTGTGAACAACATTACAGTATTGAAGTTGTATGATGGTTATTATTTTGTACAATCAATAAACTACAACAATTACACAATACGACTTGTTGACCCAAACATTCAACCCACCAATTGCTCCTCTCTCCCTCACTTCTTCTTATACAGCAACAATTTTACCCAAAAAGAAGCTAATTCATACGAAGACAGCTTATATCGATCGAATTCCACGTCTGAGTTATCTATGCCTGTAATTTACATGGAGTGTACAAGTCCACCTAGTAACGCTGTGGATAAGTATTATACGAATACAGCTTCATGCATGGGTCAACACAACATATATGTAATTGTTGGCGACCCACAACTTGGAATATTGGAGCCTCAATGCCGTGTTAAGCTTGTTACTCTCACATCCTTCTGGGGAGCATATACTTACTTCTCGGGAGCATATACTTACCACGATAACATTATAGGCAACGTATCATACATCGATATTCAGAAGGCACTAGGCTATGGATTTGAAATTTCCTGGATGCAAGCCTTTTGTCATTGTGATCATTGCTACCTCAAGGAGCCTACTGGCGAGATTCAGTGTTACCGAGACGACTACCGTAAGACCGTAGTCCTTTGTTACATTTTCATTACGTGA
Protein sequence

>Msa0104400

MVSTSNFGEKPRYARVVIVFLYLFLLPHKTYPQPCSSYCGKISNITNPFRLKHDPDHCGNSTYELDCVNNITVLKLYDGYYFVQSINYNNYTIRLVDPNIQPTNCSSLPHFFLYSNNFTQKEANSYEDSLYRSNSTSELSMPVIYMECTSPPSNAVDKYYTNTASCMGQHNIYVIVGDPQLGILEPQCRVKLVTLTSFWGAYTYFSGAYTYHDNIIGNVSYIDIQKALGYGFEISWMQAFCHCDHCYLKEPTGEIQCYRDDYRKTVVLCYIFIT*