AlfalfaGEDB | Msa0133480

M. sativa cultivar zhongmu-4 / Msa0133480

KEGG: -
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133480	XP_003592256.2	85.135	74	11	0	1	74	1	74	1.24e-28	134
Msa0133480	XP_003592256.2	89.947	567	23	1	59	625	185	717	0.0	1050

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133480	sp\|Q94A51\|PUB32_ARATH	51.542	454	203	4	174	623	362	802	3.50e-155	469

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133480	G7IES4	85.135	74	11	0	1	74	1	74	5.92e-29	134
Msa0133480	G7IES4	89.947	567	23	1	59	625	185	717	0.0	1050

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0133480	Msa0181440	0.969279	6.858496e-130	-8.615850e-47
Msa0133480	Msa0306760	0.947249	9.708997e-106	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133480	MtrunA17_Chr1g0202971	89.947	567	23	1	59	625	185	717	0.0	1050
Msa0133480	MtrunA17_Chr1g0202971	85.135	74	11	0	1	74	1	74	1.14e-32	134
Msa0133480	MtrunA17_Chr7g0247831	52.917	480	220	6	150	624	416	894	1.02e-158	478
Msa0133480	MtrunA17_Chr3g0079051	38.367	490	242	10	144	623	366	805	2.32e-100	323
Msa0133480	MtrunA17_Chr3g0079031	37.805	492	252	11	142	623	349	796	2.97e-99	320
Msa0133480	MtrunA17_Chr2g0328101	37.475	491	283	7	143	622	329	806	1.99e-96	313
Msa0133480	MtrunA17_Chr4g0070941	42.228	386	203	7	247	623	383	757	4.34e-94	306
Msa0133480	MtrunA17_Chr8g0348841	45.753	365	169	7	205	541	360	723	3.74e-89	292
Msa0133480	MtrunA17_Chr3g0135921	43.243	296	163	4	260	552	317	610	9.06e-74	248
Msa0133480	MtrunA17_Chr2g0329871	41.958	286	163	3	257	540	60	344	7.63e-73	238

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133480	AT3G49060.3	51.542	454	203	4	174	623	329	769	1.49e-156	469
Msa0133480	AT3G49060.1	51.542	454	203	4	174	623	362	802	3.55e-156	469
Msa0133480	AT2G45910.2	49.587	484	227	6	148	624	358	831	1.01e-151	458
Msa0133480	AT2G45910.1	49.587	484	227	6	148	624	358	831	1.01e-151	458
Msa0133480	AT3G49060.2	49.559	454	194	5	174	623	370	792	8.93e-144	437
Msa0133480	AT5G51270.2	43.200	375	205	6	255	623	429	801	2.77e-99	321
Msa0133480	AT5G51270.1	43.200	375	205	6	255	623	429	801	2.77e-99	321
Msa0133480	AT4G25160.1	38.017	484	259	11	176	623	354	832	2.29e-97	317
Msa0133480	AT5G61550.3	37.967	482	261	11	176	623	364	841	1.52e-96	315
Msa0133480	AT5G61550.1	37.967	482	261	11	176	623	364	841	1.52e-96	315

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Find 190 sgRNAs with CRISPR-Local

Find 643 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGTTGAAGTACAAT+TGG	0.407303	1_3:-64751633	Msa0133480:CDS
AAAATTGGAGAGGGAAGGTA+TGG	0.515519	1_3:-64751266	Msa0133480:CDS
AAACGACACCGTTTATGTTG+CGG	0.622456	1_3:-64756940	Msa0133480:CDS
AAACGTATACTTTAGATATT+TGG	0.134058	1_3:+64748859	None:intergenic
AAATATCTAAAGTATACGTT+TGG	0.419219	1_3:-64748857	Msa0133480:three_prime_UTR
AAATTCCCATGGATAATACA+AGG	0.541684	1_3:+64750355	None:intergenic
AACGAATACACGTCTGACTC+TGG	0.540992	1_3:+64750154	None:intergenic
AACTGGCATACTTAGCATTG+AGG	0.618114	1_3:-64749991	Msa0133480:CDS
AAGAAAGTAGGTGATAGAGG+GGG	0.660539	1_3:-64749027	Msa0133480:three_prime_UTR
AAGAAGACGCAATTGGAGAG+TGG	0.631865	1_3:+64757089	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTACAGAAGAAAGAGTGAA+GGG	-	chr1_3:64748970-64748989	Msa0133480:three_prime_UTR	30.0%
TTATATATAAGGGGTCGTTT+AGG	-	chr1_3:64749372-64749391	Msa0133480:CDS	30.0%
CAAAATATCTTGAACCTGTT+TGG	+	chr1_3:64749557-64749576	None:intergenic	30.0%
ACTTATTTGAACTCATCAAC+TGG	+	chr1_3:64749651-64749670	None:intergenic	30.0%
AGTAGACCAACCAAAAATAT+GGG	+	chr1_3:64749686-64749705	None:intergenic	30.0%
AAGTAGACCAACCAAAAATA+TGG	+	chr1_3:64749687-64749706	None:intergenic	30.0%
TTATAGAATTGAACTCTTGG+AGG	-	chr1_3:64749787-64749806	Msa0133480:intron	30.0%
TATAGAATTGAACTCTTGGA+GGG	-	chr1_3:64749788-64749807	Msa0133480:intron	30.0%
AAAGAAATAATACCCCTTCT+GGG	+	chr1_3:64750384-64750403	None:intergenic	30.0%
TAATATGTATCGTTATCTGC+TGG	-	chr1_3:64750438-64750457	Msa0133480:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	64748783	64757181	64748783	ID=Msa0133480;Name=Msa0133480
chr1_3	mRNA	64748783	64757181	64748783	ID=Msa0133480-mRNA-1;Parent=Msa0133480;Name=Msa0133480-mRNA-1;_AED=0.10;_eAED=0.10;_QI=160\|0.4\|0.83\|0.83\|1\|1\|6\|458\|625
chr1_3	exon	64748783	64749420	64748783	ID=Msa0133480-mRNA-1:exon:21854;Parent=Msa0133480-mRNA-1
chr1_3	exon	64749835	64750587	64749835	ID=Msa0133480-mRNA-1:exon:21853;Parent=Msa0133480-mRNA-1
chr1_3	exon	64751172	64751702	64751172	ID=Msa0133480-mRNA-1:exon:21852;Parent=Msa0133480-mRNA-1
chr1_3	exon	64752443	64752544	64752443	ID=Msa0133480-mRNA-1:exon:21851;Parent=Msa0133480-mRNA-1
chr1_3	exon	64752773	64752888	64752773	ID=Msa0133480-mRNA-1:exon:21850;Parent=Msa0133480-mRNA-1
chr1_3	exon	64756826	64757181	64756826	ID=Msa0133480-mRNA-1:exon:21849;Parent=Msa0133480-mRNA-1
chr1_3	five_prime_UTR	64757022	64757181	64757022	ID=Msa0133480-mRNA-1:five_prime_utr;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64756826	64757021	64756826	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64752773	64752888	64752773	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64752443	64752544	64752443	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64751172	64751702	64751172	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64749835	64750587	64749835	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	CDS	64749241	64749420	64749241	ID=Msa0133480-mRNA-1:cds;Parent=Msa0133480-mRNA-1
chr1_3	three_prime_UTR	64748783	64749240	64748783	ID=Msa0133480-mRNA-1:three_prime_utr;Parent=Msa0133480-mRNA-1

Gene Sequence

>Msa0133480

ATGGGTAGCATACAAGAAGCGGTATCGATTACAGAAGAAAGAGTGAAGGGTGTTGAAGAAAACGACACCGTTTATGTTGCGGTGGGATTGAACGCTGAGAAGAACAAAAAACTATTGCATTGGACAACTCACAAATTTTCTGGGAACAATATTTGCCTTCTTCACATTCATCAACCCGACCCTGTCAATTCCTACAATTCTGGTGACATGGAAGCGACTGAAAATGGCAATTCCCGCTGTTCTTTAAATGCAGAATGGTCCTTTAACAGTGTTGTTGCAAGAACAAAGTTGACAGACTTGCTTGTTCATGAGAAAAATATGCTCATTTCAATCTGTAGTCTGACATCTTCTGTCAGTTTTGGTAATTTTAGAATTGTAATTGTTTTGCAAGAGGTTGAAGATGAAAGGGAACAGGATGATGAAGAATTACAAAACTGGCGTGCTAAGGAGATAAGTCGAAGAAAAGAAGTTGAAGTACAATTGGCAAGGGAAAAACAAGACGTACAAGAAATGAAGAACCAGCGAGATAAAATAATATGTGAATTGCAAATGGTCCGAGACCAAAGTTCAGCTCTTAGAAACCAAATGTTTGAGTCCAAGTGTACGGTAACCGAGTTGGAAGAGAAGATTATATCAGCTGTGGACCTTTTGATAAGTTTCAAGGAAAAACGAGATAAGCTAAGGATTGAACATGCAAACGCAGTTAGGAAAGTTGAAGTGCTGAGAAAATTTGGAGAAGTGGATACTACATCCTCTTATGTGGTAGAATTCCCTGCATTTTCCTTTATGGAGATCAATGAGGCAACTCAGGATTTTGACCAATCTTGGAAAATTGGAGAGGGAAGGTATGGAAGTGTTTACAAGGGACTGCTTCGCAACATGCCAGTCGCCATAAAAATGCTGCCCTCTTATGGTTGTCAAAGCCAATCGGAGTTTCAGCATCAGGTAGAGGTTTTAAGTAGAGTACGACATCCAAACCTGTTAACACTAATTGGAAGCTGTGCAGAGTCTAAGTCACTTGTGTACGAATACCTAAACAACGGTAGCCTTGAAAGCCATCTTGCCTGCAAAGACAGGACTCCACTTCCATGGCAAATTCGAATATCCATTGCTACTGACATATGCTCTGCTCTAATATTCCTTCACTCCAGCAAGCCTTGTATTATCCATGGGAATTTGAAGCCTAGTAAGGTTCTCCTTGATGCTAACTTTGTAGCCAAACTTGGTGACCTGGGGATTCCTTCTTTAGTTCAGCACAATATGGATTCAGCTGACACGGGCACAGTAGTATGTAATAACTCGCATAAACGTTTGGCGTATGTGGATCCAGAGTGTCTTGTCACTGGTAAATTTACACCAGAGTCAGACGTGTATTCGTTTGGAATCATACTGTTACAACTTCTAACTGGAAGACCACTTCCGGGGCTAGTTAGAGATATGAAATGCGCATTGGAAAAGGAAAATCTTAAAACTGTGTTGGACATTTCAGCTGGTGAGTGGCCACTTCATCAAACCACACAACTGGCATACTTAGCATTGAGGTGTTGTGAAAAGACTTGGCTGGACCGGCCAGATCTTGTGTCAGAAATCTGGAGTGTTCTTAAACCATTCAGAACTATTTGCATCGACAGGAGACAAGAGTTGACTTCTAAGAAGCTTCAACGAGCTCCTTCTCATTTTGTCTGCCCCATTGTCCAGGAAGTTATGGAAGACCCATACATAGCTGCAGATGGCTTTACATATGAAGAAGAGGCAATAAGGGGATGGTTGGACAGTGGTCACAACACTTCCCCTATGACAAACCTCAAACTTGAACACACAGATTTGGTACCTAATTATGCATTACATAATGCCATTCTGGAGTGGCAGCAACTGTGA

Protein sequence

>Msa0133480

MGSIQEAVSITEERVKGVEENDTVYVAVGLNAEKNKKLLHWTTHKFSGNNICLLHIHQPDPVNSYNSGDMEATENGNSRCSLNAEWSFNSVVARTKLTDLLVHEKNMLISICSLTSSVSFGNFRIVIVLQEVEDEREQDDEELQNWRAKEISRRKEVEVQLAREKQDVQEMKNQRDKIICELQMVRDQSSALRNQMFESKCTVTELEEKIISAVDLLISFKEKRDKLRIEHANAVRKVEVLRKFGEVDTTSSYVVEFPAFSFMEINEATQDFDQSWKIGEGRYGSVYKGLLRNMPVAIKMLPSYGCQSQSEFQHQVEVLSRVRHPNLLTLIGSCAESKSLVYEYLNNGSLESHLACKDRTPLPWQIRISIATDICSALIFLHSSKPCIIHGNLKPSKVLLDANFVAKLGDLGIPSLVQHNMDSADTGTVVCNNSHKRLAYVDPECLVTGKFTPESDVYSFGIILLQLLTGRPLPGLVRDMKCALEKENLKTVLDISAGEWPLHQTTQLAYLALRCCEKTWLDRPDLVSEIWSVLKPFRTICIDRRQELTSKKLQRAPSHFVCPIVQEVMEDPYIAADGFTYEEEAIRGWLDSGHNTSPMTNLKLEHTDLVPNYALHNAILEWQQL*