Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0136200 | sp|Q9SN23|LBD38_ARATH | 67.797 | 118 | 36 | 1 | 1 | 118 | 1 | 116 | 1.77e-53 | 173 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0136200 | G7IE59 | 88.144 | 194 | 8 | 1 | 1 | 179 | 1 | 194 | 1.70e-114 | 332 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0136200 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0041800 | Msa0136200 | 0.820458 | 6.788590e-53 | -8.615850e-47 |
| Msa0089930 | Msa0136200 | 0.893171 | 8.056233e-75 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0136200 | MtrunA17_Chr1g0207471 | 88.144 | 194 | 8 | 1 | 1 | 179 | 1 | 194 | 3.27e-118 | 332 |
| Msa0136200 | MtrunA17_Chr5g0401401 | 65.079 | 126 | 41 | 2 | 1 | 126 | 1 | 123 | 5.39e-55 | 173 |
| Msa0136200 | MtrunA17_Chr4g0053911 | 69.231 | 117 | 34 | 1 | 1 | 117 | 1 | 115 | 1.51e-54 | 172 |
| Msa0136200 | MtrunA17_Chr7g0215281 | 60.577 | 104 | 41 | 0 | 1 | 104 | 3 | 106 | 1.57e-46 | 154 |
| Msa0136200 | MtrunA17_Chr6g0457431 | 59.615 | 104 | 42 | 0 | 1 | 104 | 3 | 106 | 2.26e-45 | 147 |
| Msa0136200 | MtrunA17_Chr6g0469271 | 40.000 | 160 | 79 | 4 | 1 | 152 | 3 | 153 | 5.75e-38 | 130 |
| Msa0136200 | MtrunA17_Chr6g0470651 | 48.113 | 106 | 53 | 1 | 1 | 104 | 3 | 108 | 6.37e-35 | 122 |
| Msa0136200 | MtrunA17_Chr6g0470631 | 40.945 | 127 | 68 | 2 | 1 | 125 | 3 | 124 | 2.65e-33 | 118 |
| Msa0136200 | MtrunA17_Chr1g0209841 | 40.800 | 125 | 61 | 3 | 1 | 125 | 3 | 114 | 5.17e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0136200 | AT3G49940.1 | 67.797 | 118 | 36 | 1 | 1 | 118 | 1 | 116 | 1.80e-54 | 173 |
| Msa0136200 | AT5G67420.2 | 66.379 | 116 | 37 | 1 | 1 | 116 | 1 | 114 | 1.78e-53 | 171 |
| Msa0136200 | AT5G67420.1 | 66.379 | 116 | 37 | 1 | 1 | 116 | 1 | 114 | 1.78e-53 | 171 |
| Msa0136200 | AT4G37540.1 | 54.819 | 166 | 59 | 5 | 1 | 160 | 1 | 156 | 6.23e-52 | 166 |
| Msa0136200 | AT3G02550.1 | 60.577 | 104 | 41 | 0 | 1 | 104 | 3 | 106 | 1.21e-46 | 154 |
| Msa0136200 | AT1G67100.1 | 59.048 | 105 | 43 | 0 | 1 | 105 | 3 | 107 | 5.07e-44 | 146 |
| Msa0136200 | AT1G68510.1 | 42.500 | 160 | 88 | 2 | 1 | 160 | 3 | 158 | 3.37e-42 | 141 |
Find 46 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCCCAACTGCTCCATTTAC+TGG | 0.215944 | 1_3:-68034943 | None:intergenic |
| TTTATGACATACATGATCTT+TGG | 0.296751 | 1_3:-68034707 | None:intergenic |
| AGAACTATAAATCCAGTAAA+TGG | 0.325401 | 1_3:+68034931 | Msa0136200:CDS |
| ATGGAGCAGTTGGGCTGTTA+TGG | 0.333353 | 1_3:+68034950 | Msa0136200:CDS |
| GTCTTTGTCACCAAGTTCTT+CGG | 0.371969 | 1_3:+68034642 | Msa0136200:CDS |
| GATCTTTGGTTGGGAGGAAC+AGG | 0.395798 | 1_3:-68034693 | None:intergenic |
| GACATACATGATCTTTGGTT+GGG | 0.427328 | 1_3:-68034702 | None:intergenic |
| CACTTATGTATGAAGCTGTT+GGG | 0.452221 | 1_3:+68034908 | Msa0136200:CDS |
| TGGCGTTAGCCTGTGCTTGT+GGG | 0.452316 | 1_3:-68034617 | None:intergenic |
| GACACATGAGTCTGAGGAAT+CGG | 0.458754 | 1_3:+68035128 | Msa0136200:CDS |
| TCCACACCTAACAACTGATC+AGG | 0.464754 | 1_3:-68035048 | None:intergenic |
| GGTGGCACGGCCGAAGAACT+TGG | 0.467396 | 1_3:-68034652 | None:intergenic |
| AATCCAGTAAATGGAGCAGT+TGG | 0.467406 | 1_3:+68034940 | Msa0136200:CDS |
| GAATCGGAGTCGTCCACGTT+GGG | 0.467550 | 1_3:+68035144 | Msa0136200:CDS |
| GCACTCCCTGATCAGTTGTT+AGG | 0.477941 | 1_3:+68035042 | Msa0136200:CDS |
| GTGGCGTTAGCCTGTGCTTG+TGG | 0.478831 | 1_3:-68034618 | None:intergenic |
| TGACATACATGATCTTTGGT+TGG | 0.489850 | 1_3:-68034703 | None:intergenic |
| CCCTGATCAGTTGTTAGGTG+TGG | 0.497837 | 1_3:+68035047 | Msa0136200:CDS |
| ATCCAGTAAATGGAGCAGTT+GGG | 0.536591 | 1_3:+68034941 | Msa0136200:CDS |
| TCACTTATGTATGAAGCTGT+TGG | 0.539852 | 1_3:+68034907 | Msa0136200:CDS |
| TGCATCCCTTTCTCAAAACT+CGG | 0.542491 | 1_3:-68034551 | None:intergenic |
| GAACAAGTGCTGAGAGGTAA+CGG | 0.548928 | 1_3:+68035006 | Msa0136200:CDS |
| GAGATCATAAGAATAGTGTA+AGG | 0.555999 | 1_3:-68035233 | None:intergenic |
| GATGCAAAACCCACAAGCAC+AGG | 0.559482 | 1_3:+68034608 | Msa0136200:CDS |
| TTTGAGAAAGGGATGCAGTG+AGG | 0.560200 | 1_3:+68034557 | Msa0136200:CDS |
| ATAAGAATAGTGTAAGGAGT+TGG | 0.568732 | 1_3:-68035227 | None:intergenic |
| CAGCTTCATACATAAGTGAC+TGG | 0.569517 | 1_3:-68034903 | None:intergenic |
| ATGCTACTTCTCAAACTCAA+AGG | 0.580159 | 1_3:+68035202 | Msa0136200:CDS |
| ATACATGATCTTTGGTTGGG+AGG | 0.585857 | 1_3:-68034699 | None:intergenic |
| GGCTGTTATGGACTGGAAAG+TGG | 0.603769 | 1_3:+68034962 | Msa0136200:CDS |
| GAAAAGACATGAGGGTGGCA+CGG | 0.613200 | 1_3:-68034665 | None:intergenic |
| GCAGTTGGGCTGTTATGGAC+TGG | 0.615948 | 1_3:+68034955 | Msa0136200:CDS |
| CCACACCTAACAACTGATCA+GGG | 0.634962 | 1_3:-68035047 | None:intergenic |
| TGCGGAGACACATGAGTCTG+AGG | 0.638167 | 1_3:+68035122 | Msa0136200:CDS |
| GGAATCGGAGTCGTCCACGT+TGG | 0.646426 | 1_3:+68035143 | Msa0136200:CDS |
| AAGTGGCAATTCTGTCAACT+TGG | 0.652925 | 1_3:+68034979 | Msa0136200:CDS |
| TTCTGTTCTACTTCCCAACG+TGG | 0.659625 | 1_3:-68035157 | None:intergenic |
| CAGGTGAAAGAAAAGACATG+AGG | 0.660626 | 1_3:-68034674 | None:intergenic |
| AGGTGAAAGAAAAGACATGA+GGG | 0.666800 | 1_3:-68034673 | None:intergenic |
| TAACAACTGATCAGGGAGTG+CGG | 0.677605 | 1_3:-68035040 | None:intergenic |
| CCAAGCAGAAAAGCAACGTG+CGG | 0.683012 | 1_3:+68035104 | Msa0136200:CDS |
| TGCTACGAGATTGTCTAACG+TGG | 0.703821 | 1_3:+68034586 | Msa0136200:CDS |
| GAACTTGGTGACAAAGACAG+TGG | 0.712149 | 1_3:-68034637 | None:intergenic |
| TGAAAGAAAAGACATGAGGG+TGG | 0.725920 | 1_3:-68034670 | None:intergenic |
| GGTGTTGAACAAGTGCTGAG+AGG | 0.756300 | 1_3:+68035000 | Msa0136200:CDS |
| CAAGTGCTGAGAGGTAACGG+CGG | 0.757236 | 1_3:+68035009 | Msa0136200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACAAATTAGTTTGATGTATT+TGG | - | chr1_3:68034792-68034811 | None:intergenic | 20.0% |
| !!! | ACTAATTTGTATGTATATAC+AGG | + | chr1_3:68034802-68034821 | Msa0136200:intron | 20.0% |
| !! | TCATTCAATTACTATATTAC+AGG | + | chr1_3:68034874-68034893 | Msa0136200:intron | 20.0% |
| ! | TTTATGACATACATGATCTT+TGG | - | chr1_3:68034710-68034729 | None:intergenic | 25.0% |
| ! | AGAACTATAAATCCAGTAAA+TGG | + | chr1_3:68034931-68034950 | Msa0136200:CDS | 25.0% |
| ATAAGAATAGTGTAAGGAGT+TGG | - | chr1_3:68035230-68035249 | None:intergenic | 30.0% | |
| AGGTGAAAGAAAAGACATGA+GGG | - | chr1_3:68034676-68034695 | None:intergenic | 35.0% | |
| GACATACATGATCTTTGGTT+GGG | - | chr1_3:68034705-68034724 | None:intergenic | 35.0% | |
| TGACATACATGATCTTTGGT+TGG | - | chr1_3:68034706-68034725 | None:intergenic | 35.0% | |
| TCACTTATGTATGAAGCTGT+TGG | + | chr1_3:68034907-68034926 | Msa0136200:CDS | 35.0% | |
| CACTTATGTATGAAGCTGTT+GGG | + | chr1_3:68034908-68034927 | Msa0136200:CDS | 35.0% | |
| ! | ATGCTACTTCTCAAACTCAA+AGG | + | chr1_3:68035202-68035221 | Msa0136200:CDS | 35.0% |
| TGCATCCCTTTCTCAAAACT+CGG | - | chr1_3:68034554-68034573 | None:intergenic | 40.0% | |
| GTCTTTGTCACCAAGTTCTT+CGG | + | chr1_3:68034642-68034661 | Msa0136200:CDS | 40.0% | |
| TGAAAGAAAAGACATGAGGG+TGG | - | chr1_3:68034673-68034692 | None:intergenic | 40.0% | |
| CAGGTGAAAGAAAAGACATG+AGG | - | chr1_3:68034677-68034696 | None:intergenic | 40.0% | |
| ATACATGATCTTTGGTTGGG+AGG | - | chr1_3:68034702-68034721 | None:intergenic | 40.0% | |
| CAGCTTCATACATAAGTGAC+TGG | - | chr1_3:68034906-68034925 | None:intergenic | 40.0% | |
| AATCCAGTAAATGGAGCAGT+TGG | + | chr1_3:68034940-68034959 | Msa0136200:CDS | 40.0% | |
| ATCCAGTAAATGGAGCAGTT+GGG | + | chr1_3:68034941-68034960 | Msa0136200:CDS | 40.0% | |
| AAGTGGCAATTCTGTCAACT+TGG | + | chr1_3:68034979-68034998 | Msa0136200:CDS | 40.0% | |
| !!! | GGTTGCCGAGTTTTGAGAAA+GGG | + | chr1_3:68034546-68034565 | Msa0136200:CDS | 45.0% |
| TTTGAGAAAGGGATGCAGTG+AGG | + | chr1_3:68034557-68034576 | Msa0136200:CDS | 45.0% | |
| TGCTACGAGATTGTCTAACG+TGG | + | chr1_3:68034586-68034605 | Msa0136200:CDS | 45.0% | |
| ! | GAACTTGGTGACAAAGACAG+TGG | - | chr1_3:68034640-68034659 | None:intergenic | 45.0% |
| !! | GAACAAGTGCTGAGAGGTAA+CGG | + | chr1_3:68035006-68035025 | Msa0136200:CDS | 45.0% |
| TAACAACTGATCAGGGAGTG+CGG | - | chr1_3:68035043-68035062 | None:intergenic | 45.0% | |
| CCACACCTAACAACTGATCA+GGG | - | chr1_3:68035050-68035069 | None:intergenic | 45.0% | |
| TCCACACCTAACAACTGATC+AGG | - | chr1_3:68035051-68035070 | None:intergenic | 45.0% | |
| GACACATGAGTCTGAGGAAT+CGG | + | chr1_3:68035128-68035147 | Msa0136200:CDS | 45.0% | |
| TTCTGTTCTACTTCCCAACG+TGG | - | chr1_3:68035160-68035179 | None:intergenic | 45.0% | |
| !!! | CGGTTGCCGAGTTTTGAGAA+AGG | + | chr1_3:68034545-68034564 | Msa0136200:CDS | 50.0% |
| GATGCAAAACCCACAAGCAC+AGG | + | chr1_3:68034608-68034627 | Msa0136200:CDS | 50.0% | |
| GAAAAGACATGAGGGTGGCA+CGG | - | chr1_3:68034668-68034687 | None:intergenic | 50.0% | |
| GATCTTTGGTTGGGAGGAAC+AGG | - | chr1_3:68034696-68034715 | None:intergenic | 50.0% | |
| AGCCCAACTGCTCCATTTAC+TGG | - | chr1_3:68034946-68034965 | None:intergenic | 50.0% | |
| ATGGAGCAGTTGGGCTGTTA+TGG | + | chr1_3:68034950-68034969 | Msa0136200:CDS | 50.0% | |
| !! | GGCTGTTATGGACTGGAAAG+TGG | + | chr1_3:68034962-68034981 | Msa0136200:CDS | 50.0% |
| !! | GGTGTTGAACAAGTGCTGAG+AGG | + | chr1_3:68035000-68035019 | Msa0136200:CDS | 50.0% |
| GCACTCCCTGATCAGTTGTT+AGG | + | chr1_3:68035042-68035061 | Msa0136200:CDS | 50.0% | |
| CCCTGATCAGTTGTTAGGTG+TGG | + | chr1_3:68035047-68035066 | Msa0136200:CDS | 50.0% | |
| CCAAGCAGAAAAGCAACGTG+CGG | + | chr1_3:68035104-68035123 | Msa0136200:CDS | 50.0% | |
| ! | TGGCGTTAGCCTGTGCTTGT+GGG | - | chr1_3:68034620-68034639 | None:intergenic | 55.0% |
| GCAGTTGGGCTGTTATGGAC+TGG | + | chr1_3:68034955-68034974 | Msa0136200:CDS | 55.0% | |
| !! | CAAGTGCTGAGAGGTAACGG+CGG | + | chr1_3:68035009-68035028 | Msa0136200:CDS | 55.0% |
| !!! | CCGCACGTTGCTTTTCTGCT+TGG | - | chr1_3:68035107-68035126 | None:intergenic | 55.0% |
| TGCGGAGACACATGAGTCTG+AGG | + | chr1_3:68035122-68035141 | Msa0136200:CDS | 55.0% | |
| GAATCGGAGTCGTCCACGTT+GGG | + | chr1_3:68035144-68035163 | Msa0136200:CDS | 55.0% | |
| ! | GTGGCGTTAGCCTGTGCTTG+TGG | - | chr1_3:68034621-68034640 | None:intergenic | 60.0% |
| GGAATCGGAGTCGTCCACGT+TGG | + | chr1_3:68035143-68035162 | Msa0136200:CDS | 60.0% | |
| GGTGGCACGGCCGAAGAACT+TGG | - | chr1_3:68034655-68034674 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 68034534 | 68035251 | 68034534 | ID=Msa0136200;Name=Msa0136200 |
| chr1_3 | mRNA | 68034534 | 68035251 | 68034534 | ID=Msa0136200-mRNA-1;Parent=Msa0136200;Name=Msa0136200-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|2|0|179 |
| chr1_3 | exon | 68034534 | 68034717 | 68034534 | ID=Msa0136200-mRNA-1:exon:23459;Parent=Msa0136200-mRNA-1 |
| chr1_3 | exon | 68034896 | 68035251 | 68034896 | ID=Msa0136200-mRNA-1:exon:23460;Parent=Msa0136200-mRNA-1 |
| chr1_3 | CDS | 68034534 | 68034717 | 68034534 | ID=Msa0136200-mRNA-1:cds;Parent=Msa0136200-mRNA-1 |
| chr1_3 | CDS | 68034896 | 68035251 | 68034896 | ID=Msa0136200-mRNA-1:cds;Parent=Msa0136200-mRNA-1 |
| Gene Sequence |
| Protein sequence |
