AlfalfaGEDB | Msa0136970

M. sativa cultivar zhongmu-4 / Msa0136970

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0136970	GAU14792.1	63.736	91	33	0	1	91	1	91	2.44e-25	122
Msa0136970	GAU14792.1	86.463	458	59	2	84	540	167	622	0.0	823

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0136970	sp\|Q3ECH2\|LRL28_ARATH	52.577	388	153	8	164	536	492	863	5.07e-125	390

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0136970	A0A2Z6LWT2	63.736	91	33	0	1	91	1	91	1.17e-25	122
Msa0136970	A0A2Z6LWT2	86.463	458	59	2	84	540	167	622	0.0	823

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0136960	Msa0136970	0.881167	3.004670e-70	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0136970	MtrunA17_Chr1g0209471	94.293	368	21	0	175	542	1	368	0.0	719
Msa0136970	MtrunA17_Chr7g0232441	87.871	371	45	0	172	542	258	628	0.0	684
Msa0136970	MtrunA17_Chr7g0232421	88.859	368	41	0	175	542	1	368	0.0	684
Msa0136970	MtrunA17_Chr1g0209401	89.415	359	37	1	184	542	1	358	0.0	665
Msa0136970	MtrunA17_Chr1g0209501	60.177	452	164	4	91	542	178	613	0.0	562
Msa0136970	MtrunA17_Chr1g0209511	60.356	449	157	6	85	527	175	608	0.0	553
Msa0136970	MtrunA17_Chr1g0208841	77.174	368	20	2	175	542	1	304	0.0	526
Msa0136970	MtrunA17_Chr6g0483981	59.621	369	143	5	166	533	199	562	9.28e-153	449
Msa0136970	MtrunA17_Chr6g0484071	60.674	356	134	3	180	533	2	353	1.16e-152	441
Msa0136970	MtrunA17_Chr6g0484041	58.967	368	147	4	166	533	254	617	1.92e-151	447

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0136970	AT1G70250.1	60.559	322	116	5	226	541	448	764	4.13e-126	388
Msa0136970	AT1G67000.1	52.577	388	153	8	164	536	492	863	5.15e-126	390
Msa0136970	AT5G38260.1	49.034	414	183	9	119	525	215	607	2.48e-125	381
Msa0136970	AT4G18250.1	59.807	311	112	5	226	528	344	649	6.65e-125	382
Msa0136970	AT1G66910.2	48.244	427	199	10	124	539	230	645	5.25e-123	376
Msa0136970	AT1G66910.1	51.872	374	166	6	170	539	285	648	8.37e-121	370
Msa0136970	AT1G66920.2	50.376	399	175	8	141	527	207	594	1.15e-120	369
Msa0136970	AT1G66920.1	53.352	358	147	6	180	527	239	586	8.16e-120	366
Msa0136970	AT5G38280.1	58.065	310	119	5	226	530	320	623	1.11e-119	367
Msa0136970	AT5G38280.2	58.065	310	119	5	226	530	320	623	1.86e-119	367

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Find 109 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTAGTGGAAATGTTGGA+AGG	0.582199	1_3:+69247061	Msa0136970:CDS
AAATCAGATGTCTACAGTTA+TGG	0.433327	1_3:+69246812	Msa0136970:CDS
AAATGAAATCGATGAAGAAA+TGG	0.408910	1_3:+69246970	Msa0136970:CDS
AAATGACAGTGGTGAGTTTA+TGG	0.304569	1_3:+69246999	Msa0136970:CDS
AAATGTCTGGAGAACAACTC+TGG	0.527828	1_3:-69246773	None:intergenic
AAATTAGGTCAGGGAGGGTA+CGG	0.522410	1_3:+69246266	Msa0136970:CDS
AACTCTGGAGCAATATATCC+TGG	0.517007	1_3:-69246758	None:intergenic
AACTGAAGTACATATAACCA+AGG	0.666097	1_3:+69245592	Msa0136970:CDS
AACTTCTCAAGAGATCCATT+AGG	0.631628	1_3:-69246479	None:intergenic
AAGTAGTGGAAATGTTGGAA+GGG	0.610087	1_3:+69247062	Msa0136970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATCCACAACTAAATGAATC+TGG	-	chr1_3:69245046-69245065	None:intergenic	30.0%
TCATTTAGTTGTGGATATCT+TGG	+	chr1_3:69245049-69245068	Msa0136970:CDS	30.0%
AATATCAGAATAAAGGGGAA+TGG	+	chr1_3:69245170-69245189	Msa0136970:CDS	30.0%
AAAGAAACAAAGTCAGAAGT+GGG	-	chr1_3:69245338-69245357	None:intergenic	30.0%
TGGTTATATGTACTTCAGTT+TGG	-	chr1_3:69245592-69245611	None:intergenic	30.0%
AACTGAAGTACATATAACCA+AGG	+	chr1_3:69245592-69245611	Msa0136970:CDS	30.0%
CAGTGTCAAATTGATAGAAA+AGG	+	chr1_3:69245647-69245666	Msa0136970:CDS	30.0%
GATATGGTTAGTAAGAAAGA+AGG	+	chr1_3:69245797-69245816	Msa0136970:intron	30.0%
TAGAAAGCCAAAATTTAGTC+TGG	+	chr1_3:69245945-69245964	Msa0136970:intron	30.0%
TTCAACCCATCAAATTATTG+AGG	+	chr1_3:69246160-69246179	Msa0136970:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	69244989	69247171	69244989	ID=Msa0136970;Name=Msa0136970
chr1_3	mRNA	69244989	69247171	69244989	ID=Msa0136970-mRNA-1;Parent=Msa0136970;Name=Msa0136970-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|542
chr1_3	exon	69244989	69245236	69244989	ID=Msa0136970-mRNA-1:exon:23941;Parent=Msa0136970-mRNA-1
chr1_3	exon	69245478	69245695	69245478	ID=Msa0136970-mRNA-1:exon:23942;Parent=Msa0136970-mRNA-1
chr1_3	exon	69245798	69245848	69245798	ID=Msa0136970-mRNA-1:exon:23943;Parent=Msa0136970-mRNA-1
chr1_3	exon	69246060	69247171	69246060	ID=Msa0136970-mRNA-1:exon:23944;Parent=Msa0136970-mRNA-1
chr1_3	CDS	69244989	69245236	69244989	ID=Msa0136970-mRNA-1:cds;Parent=Msa0136970-mRNA-1
chr1_3	CDS	69245478	69245695	69245478	ID=Msa0136970-mRNA-1:cds;Parent=Msa0136970-mRNA-1
chr1_3	CDS	69245798	69245848	69245798	ID=Msa0136970-mRNA-1:cds;Parent=Msa0136970-mRNA-1
chr1_3	CDS	69246060	69247171	69246060	ID=Msa0136970-mRNA-1:cds;Parent=Msa0136970-mRNA-1

Gene Sequence

>Msa0136970

ATGATGCTACTTGTGTTGGTGGGTGGTGATGAGAGTAAGTACCAAGGAGAGTGTCCAGATTCATTTAGTTGTGGATATCTTGGCAAAATCAGCTTCCCTTTCACTACAACAGAACGCCAGGACTGTGGCTTATTGCCAATACCTAACTGTGATGGTGATCCAATACAGCCCAAAATGATCAAATATCAGAATAAAGGGGAATGGTTTGAGTTTGAGGTTGAACTTGTAACTCCTCGAGTTTCACAGTGCTACGCTGATAATGCGTCTCGGAGTGCTTTTACATCTTGTACAAATGTCCAGCTTCCAGTTAAAGACTTTGCTGATGGCGATGACCCTTTTACTTTCGTAACTGCTGATATCCAAACTGAAGTACATATAACCAAGGAATGTGCATATTGTTACTTCAACCAAAGTGGACAGTGTCAAATTGATAGAAAAGGGAGGTTCTATTGTGCCAATGCTAATGATATGGTTAGTAAGAAAGAAGGGCTGACAAGGAATGTCAAACTGGGTATAGCAGCCATGATAGCTATTGGAGTTGCAGTGCTGATGTTGTTGGCTTACTGCATTATGAGAAAGGTCTTTCCTCCAGCATTTCTTTTGTTCAGGAAGGAGAGTTCAACCCATCAAATTATTGAGGAATTCTTGAAAGAACATGGACCTCTTCCAGCTGCAAGGTACAGTTATTCTGATGTCACAAAAATGACCAACTGTTTTAGAAACAAATTAGGTCAGGGAGGGTACGGAAGTGTATACAAAGGGAAGTTACATGATGAGCGTATTGTTGCAGTGAAAGTTTTAAGTGAATCAAAAGGCGATGGCGAAGATTTTATTAATGAAGTTGCGAGTATCAGTAGAACTTCACACGTCAACGTTGTTAGACTTTTAGGGTTCTGTTTGGATGGTTCTAAAAAGGCATTGATATACGAATTCATGCCTAATGGATCTCTTGAGAAGTTCATATATGAAGAGAAAAATCCATTGAGGGATGATCGCCAATTGGATTGCAAAACATTGTATGATATTGCAGTTGGCGTTGCTCGTGGATTGGAGTACTTGCACAGAGGCTGTAACACTAGAATCTTGCATTTTGACATAAAACCTCATAATATATTACTAGATGATGATTTTTGTCCCAAAATTTCAGATTTTGGACTTTCTAAAGTATGTCCACGAAAAGAAAGCATTGTATCTATATTTGGTGCAAGGGGAACACCAGGATATATTGCTCCAGAGTTGTTCTCCAGACATTTTGGTGGGGTGTCACATAAATCAGATGTCTACAGTTATGGAATGATGGTTTTAGAAATGGTTGGTCGAAGAAAGAACATTAAGGTTGAAGTTGATTGTTCTAGCAAGTTATATTTTCCTCACTGGATTTACAAGCGTCTTGAATTGAATCAGGATCTTGGACTCAGGTGTATTAAAAATGAAATCGATGAAGAAATGGTAAGAAAAATGACAGTGGTGAGTTTATGGTGCATACAAACTGACCCTTCACATCGACCAGCAATGCATAAAGTAGTGGAAATGTTGGAAGGGAGCCTTCAAGTGCTGGAAATACCACCCAAGCCCTTTTTGTCTTCTCCTTCAACATCACCTATCAATCTATCATCTGAAATATTGTAA

Protein sequence

>Msa0136970

MMLLVLVGGDESKYQGECPDSFSCGYLGKISFPFTTTERQDCGLLPIPNCDGDPIQPKMIKYQNKGEWFEFEVELVTPRVSQCYADNASRSAFTSCTNVQLPVKDFADGDDPFTFVTADIQTEVHITKECAYCYFNQSGQCQIDRKGRFYCANANDMVSKKEGLTRNVKLGIAAMIAIGVAVLMLLAYCIMRKVFPPAFLLFRKESSTHQIIEEFLKEHGPLPAARYSYSDVTKMTNCFRNKLGQGGYGSVYKGKLHDERIVAVKVLSESKGDGEDFINEVASISRTSHVNVVRLLGFCLDGSKKALIYEFMPNGSLEKFIYEEKNPLRDDRQLDCKTLYDIAVGVARGLEYLHRGCNTRILHFDIKPHNILLDDDFCPKISDFGLSKVCPRKESIVSIFGARGTPGYIAPELFSRHFGGVSHKSDVYSYGMMVLEMVGRRKNIKVEVDCSSKLYFPHWIYKRLELNQDLGLRCIKNEIDEEMVRKMTVVSLWCIQTDPSHRPAMHKVVEMLEGSLQVLEIPPKPFLSSPSTSPINLSSEIL*