AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142400 MtrunA17_Chr8g0383981 95.858 507 21 0 1 507 1 507 0.0 1005
Msa0142400 MtrunA17_Chr8g0383561 86.364 154 19 1 1 154 1 152 3.73e-87 265
Msa0142400 MtrunA17_Chr3g0114841 38.095 399 194 12 136 496 117 500 6.17e-66 221
Msa0142400 MtrunA17_Chr5g0424131 38.060 402 210 14 125 496 107 499 7.77e-65 218
Msa0142400 MtrunA17_Chr4g0038761 32.796 372 219 12 144 499 89 445 8.21e-42 155
Msa0142400 MtrunA17_Chr2g0306641 30.952 378 230 13 136 495 122 486 6.29e-41 154
Msa0142400 MtrunA17_Chr3g0110971 32.905 389 214 15 136 495 216 586 8.38e-40 152
Msa0142400 MtrunA17_Chr7g0224271 30.958 407 223 15 126 495 287 672 5.74e-39 150
Msa0142400 MtrunA17_Chr4g0057291 29.177 401 233 16 136 504 289 670 2.02e-36 143
Msa0142400 MtrunA17_Chr8g0376381 29.708 377 227 12 136 495 166 521 1.24e-35 139
Msa0142400 MtrunA17_Chr7g0245601 27.614 373 229 14 144 497 443 793 3.51e-33 134
Msa0142400 MtrunA17_Chr4g0039661 31.818 374 216 17 144 495 198 554 3.91e-31 126
Msa0142400 MtrunA17_Chr1g0200401 30.633 395 226 18 136 495 77 458 3.00e-27 114
Msa0142400 MtrunA17_Chr7g0230731 27.078 373 251 9 133 495 366 727 7.06e-27 114
Msa0142400 MtrunA17_Chr1g0183641 26.042 384 237 13 135 496 119 477 4.82e-26 110
Msa0142400 MtrunA17_Chr2g0319091 27.604 384 240 16 136 495 209 578 7.88e-26 111
Msa0142400 MtrunA17_Chr4g0076091 25.189 397 261 14 123 496 160 543 1.74e-25 109
Msa0142400 MtrunA17_Chr7g0230771 26.542 373 253 9 133 495 369 730 2.92e-25 110
Msa0142400 MtrunA17_Chr8g0357601 26.823 384 246 13 136 495 179 551 4.42e-25 108
Msa0142400 MtrunA17_Chr5g0445331 25.542 415 268 15 111 495 128 531 5.74e-25 108
Msa0142400 MtrunA17_Chr4g0031711 25.862 406 248 17 127 498 349 735 1.76e-24 107
Msa0142400 MtrunA17_Chr2g0294461 27.160 405 243 16 126 495 197 584 1.96e-24 107
Msa0142400 MtrunA17_Chr5g0447691 27.518 407 253 18 114 495 154 543 3.54e-24 105
Msa0142400 MtrunA17_Chr3g0101141 27.157 394 254 16 123 495 160 541 3.67e-24 105
Msa0142400 MtrunA17_Chr5g0401131 26.684 386 250 16 138 497 113 491 4.87e-24 105
Msa0142400 MtrunA17_Chr3g0125811 26.649 379 244 12 139 495 203 569 1.17e-23 104
Msa0142400 MtrunA17_Chr1g0159461 27.368 380 240 14 139 495 225 591 2.30e-23 103
Msa0142400 MtrunA17_Chr7g0238891 25.485 412 259 17 115 498 351 742 2.56e-23 103
Msa0142400 MtrunA17_Chr2g0328391 24.623 398 261 16 127 498 287 671 3.29e-23 103
Msa0142400 MtrunA17_Chr2g0328431 24.474 380 245 16 144 498 294 656 6.74e-23 102
Msa0142400 MtrunA17_Chr4g0031741 25.648 386 244 15 139 498 284 652 8.68e-23 102
Msa0142400 MtrunA17_Chr2g0290241 26.257 358 220 15 168 495 81 424 1.01e-22 100
Msa0142400 MtrunA17_Chr2g0294471 26.980 404 242 17 126 495 191 575 1.13e-22 101
Msa0142400 MtrunA17_Chr4g0057891 27.692 390 236 16 136 495 146 519 1.48e-21 97.8
Msa0142400 MtrunA17_Chr2g0328411 24.000 375 249 16 144 496 294 654 2.92e-21 97.4
Msa0142400 MtrunA17_Chr4g0031701 24.495 396 248 16 136 498 251 628 4.54e-20 93.6
Msa0142400 MtrunA17_Chr4g0012071 26.702 382 240 16 133 495 190 550 1.56e-18 88.6
Msa0142400 MtrunA17_Chr4g0031731 25.231 325 200 13 136 434 251 558 4.15e-18 87.4
Msa0142400 MtrunA17_Chr2g0328381 22.727 396 277 14 122 498 258 643 5.46e-18 87.0
Msa0142400 MtrunA17_Chr8g0373561 27.005 374 247 13 136 495 179 540 7.22e-18 86.7
Msa0142400 MtrunA17_Chr4g0031721 25.570 395 241 18 136 498 313 686 1.10e-17 86.3
Msa0142400 MtrunA17_Chr2g0328341 23.969 388 262 15 132 498 265 640 2.08e-17 85.5
Msa0142400 MtrunA17_Chr7g0270031 28.523 298 174 14 219 495 274 553 1.39e-15 79.3
Msa0142400 MtrunA17_Chr5g0401421 22.248 427 252 12 139 498 35 448 3.17e-15 77.8
Msa0142400 MtrunA17_Chr2g0329591 24.444 360 216 14 177 497 110 452 1.66e-14 75.9
Msa0142400 MtrunA17_Chr1g0194621 25.062 403 251 17 126 495 133 517 2.29e-14 75.5
Msa0142400 MtrunA17_Chr2g0328361 22.670 397 251 14 122 498 257 617 1.21e-13 73.6
Msa0142400 MtrunA17_Chr5g0396491 29.834 181 116 7 136 310 53 228 1.37e-13 72.8
Msa0142400 MtrunA17_Chr4g0053901 22.565 421 254 14 139 497 59 469 3.34e-13 71.6
Msa0142400 MtrunA17_Chr4g0038851 28.155 206 134 7 136 332 45 245 2.04e-12 69.3
Msa0142400 MtrunA17_Chr2g0328371 24.473 237 154 7 211 433 5 230 2.81e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142400 AT4G08250.1 32.905 389 228 14 136 501 104 482 1.09e-47 172
Msa0142400 AT1G55580.1 35.238 315 162 12 209 495 144 444 4.10e-42 156
Msa0142400 AT5G41920.1 32.275 378 220 14 136 495 40 399 1.47e-40 151
Msa0142400 AT3G03450.1 29.973 377 235 13 136 495 180 544 5.19e-37 144
Msa0142400 AT1G66350.1 29.178 377 227 14 136 495 152 505 2.05e-35 139
Msa0142400 AT5G17490.1 30.311 386 230 14 130 495 149 515 8.51e-35 137
Msa0142400 AT2G01570.1 29.351 385 222 16 136 495 221 580 1.07e-32 132
Msa0142400 AT1G14920.1 28.424 387 223 16 136 495 169 528 1.16e-31 128
Msa0142400 AT3G54220.1 27.394 376 229 14 144 497 298 651 5.90e-31 127
Msa0142400 AT1G63100.1 25.000 404 251 14 129 496 242 629 4.30e-30 124
Msa0142400 AT5G66770.1 29.738 343 203 12 177 495 255 583 4.51e-30 124
Msa0142400 AT1G63100.2 25.000 404 251 14 129 496 267 654 7.31e-30 124
Msa0142400 AT5G59450.1 27.475 404 241 18 136 506 224 608 8.52e-30 123
Msa0142400 AT3G50650.1 29.661 354 211 14 167 495 201 541 8.62e-29 120
Msa0142400 AT4G17230.1 29.073 399 238 16 128 497 144 526 1.22e-28 119
Msa0142400 AT4G17230.2 29.073 399 238 16 128 497 144 526 1.22e-28 119
Msa0142400 AT2G04890.1 28.465 404 244 18 119 495 27 412 9.98e-28 115
Msa0142400 AT3G46600.2 28.278 389 233 15 138 498 3 373 7.99e-27 112
Msa0142400 AT1G50600.3 26.166 386 257 13 129 495 149 525 8.30e-27 114
Msa0142400 AT3G46600.1 27.423 423 256 16 103 497 181 580 8.55e-27 114
Msa0142400 AT3G46600.3 27.423 423 256 16 103 497 149 548 8.74e-27 114
Msa0142400 AT1G50600.2 26.166 386 257 13 129 495 224 600 1.74e-26 113
Msa0142400 AT1G50600.1 26.166 386 257 13 129 495 220 596 1.91e-26 113
Msa0142400 AT5G48150.1 27.833 406 247 16 118 495 102 489 2.10e-26 112
Msa0142400 AT5G48150.4 27.833 406 247 16 118 495 102 489 2.10e-26 112
Msa0142400 AT5G48150.2 27.833 406 247 16 118 495 102 489 2.10e-26 112
Msa0142400 AT5G48150.3 27.833 406 247 16 118 495 102 489 2.10e-26 112
Msa0142400 AT1G07530.1 26.698 427 253 18 104 498 369 767 3.06e-25 110
Msa0142400 AT1G21450.1 27.356 435 270 18 87 495 178 592 1.49e-24 107
Msa0142400 AT4G37650.1 26.531 392 250 14 139 498 146 531 3.75e-24 106
Msa0142400 AT2G29060.2 26.617 402 237 20 132 497 277 656 8.99e-23 102
Msa0142400 AT2G29060.1 26.617 402 237 20 132 497 314 693 9.00e-23 102
Msa0142400 AT2G37650.2 24.490 392 263 12 127 497 323 702 2.20e-21 98.2
Msa0142400 AT2G37650.1 24.490 392 263 12 127 497 335 714 2.55e-21 98.2
Msa0142400 AT4G00150.1 27.851 377 229 17 135 495 204 553 2.41e-20 94.7
Msa0142400 AT1G07520.1 23.907 389 256 14 139 498 318 695 4.32e-18 88.2
Msa0142400 AT1G07520.3 23.907 389 256 14 139 498 319 696 4.44e-18 88.2
Msa0142400 AT1G07520.2 23.907 389 256 14 139 498 154 531 7.46e-18 87.0
Msa0142400 AT1G07520.4 23.907 389 256 14 139 498 154 531 7.46e-18 87.0
Msa0142400 AT4G36710.1 27.100 369 249 14 138 495 125 484 1.07e-17 86.3
Msa0142400 AT5G52510.1 26.667 315 191 11 212 495 334 639 3.06e-17 85.5
Msa0142400 AT2G29065.1 24.768 323 201 12 203 498 323 630 3.73e-17 85.1
Msa0142400 AT1G50420.1 23.733 434 248 16 136 495 54 478 4.04e-17 84.3
Msa0142400 AT2G45160.1 24.365 394 226 17 141 495 278 638 7.88e-16 80.9
Msa0142400 AT3G49950.1 24.103 390 255 13 139 497 30 409 1.82e-15 79.0
Msa0142400 AT3G60630.1 24.451 319 184 12 209 495 328 621 1.60e-13 73.6

Find 77 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACCTCTTTCTCTATA+AGG 0.158083 1_4:-1306622 None:intergenic
ATTTCATCTAGGATTGTATT+TGG 0.213674 1_4:-1305516 None:intergenic
ATTTCGTCTAGGATTGTATT+TGG 0.226044 1_4:-1305576 None:intergenic
CTAAACAATGCCTCGTCTTT+AGG 0.252519 1_4:+1305882 Msa0142400:CDS
GGAAGTTCAAGAAGAGTTAA+TGG 0.258644 1_4:+1305770 Msa0142400:CDS
CTGTGATTGCTGTTACTCTA+AGG 0.272598 1_4:-1306208 None:intergenic
GCTTGTTTGGTAAAGGGTAT+TGG 0.274576 1_4:+1306792 Msa0142400:CDS
AAGCTATTGGTTAGCTTGTT+TGG 0.278423 1_4:+1306779 Msa0142400:CDS
AACAAGCCTTGGTGATGATT+TGG 0.290948 1_4:-1306453 None:intergenic
CTTTGACTTCTGTTTCAATC+TGG 0.297827 1_4:+1306861 Msa0142400:CDS
CTTGTTTGGTAAAGGGTATT+GGG 0.313644 1_4:+1306793 Msa0142400:CDS
CCAGGGAAGAAGATTCTTAT+TGG 0.318155 1_4:+1305745 Msa0142400:CDS
GCGAAAATGTGTTTGTTTCC+AGG 0.325069 1_4:+1305727 Msa0142400:CDS
CAATACCTCTTTCTCTATAA+GGG 0.331359 1_4:-1306621 None:intergenic
TGTGGTGGAGTTACCGAAAA+AGG 0.334912 1_4:+1305702 Msa0142400:CDS
TATTTCACAGGCTAAGCTAT+TGG 0.357597 1_4:+1306766 Msa0142400:CDS
CTCATAATCCATCACATTCA+TGG 0.366173 1_4:-1305397 None:intergenic
TGGTTAGCTTGTTTGGTAAA+GGG 0.385802 1_4:+1306786 Msa0142400:CDS
TAAGGGCTAACTCCATCTTA+TGG 0.393772 1_4:-1306604 None:intergenic
AAGGCGATAGTTTATTGAAC+AGG 0.393824 1_4:+1305907 Msa0142400:CDS
CCTTATAGAGAAAGAGGTAT+TGG 0.394224 1_4:+1306622 Msa0142400:CDS
GATCAAACAATATTTCATCT+AGG 0.398157 1_4:-1305527 None:intergenic
AATCCAATGGCCACCGTTGA+TGG 0.402840 1_4:+1306154 Msa0142400:CDS
TTGGTTAGCTTGTTTGGTAA+AGG 0.407587 1_4:+1306785 Msa0142400:CDS
ATCAATGAGAAACTCCTGTT+TGG 0.411022 1_4:-1306407 None:intergenic
ACTCAAATCCATGTCCTTTG+TGG 0.411956 1_4:+1306511 Msa0142400:CDS
TTTGACTTCTGTTTCAATCT+GGG 0.412837 1_4:+1306862 Msa0142400:CDS
TACATCAGTGGATGCCAAAC+AGG 0.416877 1_4:+1306393 Msa0142400:CDS
AGCTTCGAAAATCGCTTGAT+TGG 0.418613 1_4:-1306081 None:intergenic
CTAAGCTACATTCATGCAGC+TGG 0.426844 1_4:-1305455 None:intergenic
CTTATAGAGAAAGAGGTATT+GGG 0.435957 1_4:+1306623 Msa0142400:CDS
GAGATAAGAGATGTTGTGAT+TGG 0.464477 1_4:-1305554 None:intergenic
CAACCATCAACGGTGGCCAT+TGG 0.475147 1_4:-1306157 None:intergenic
CCAATAAGAATCTTCTTCCC+TGG 0.475253 1_4:-1305745 None:intergenic
GAGACATGAGATCTTCTGCT+TGG 0.478088 1_4:-1305494 None:intergenic
CGAAAATGTGTTTGTTTCCA+GGG 0.485498 1_4:+1305728 Msa0142400:CDS
GTTAGCCCTTATAGAGAAAG+AGG 0.487244 1_4:+1306616 Msa0142400:CDS
AATGCCTCGTCTTTAGGAAA+AGG 0.493064 1_4:+1305888 Msa0142400:CDS
TCGACTAAAACAACAAGCCT+TGG 0.505560 1_4:-1306464 None:intergenic
ACATGTTCTGAATGGAACTT+AGG 0.509744 1_4:-1305978 None:intergenic
GCAATATGAGAACTGTAAGT+TGG 0.510443 1_4:+1306817 Msa0142400:CDS
AAAAGAGGAGAGAATATTGT+GGG 0.510893 1_4:+1306685 Msa0142400:CDS
GAAGCCTCAGTTCAACAAAC+AGG 0.521298 1_4:+1306245 Msa0142400:CDS
TTACTCATATTATAATCTGT+TGG 0.522232 1_4:-1306887 None:intergenic
ACTGTAAGTTGGCTTTGTGT+TGG 0.525441 1_4:+1306828 Msa0142400:CDS
GAATTCAAAGAAATTGAACT+TGG 0.527275 1_4:+1306344 Msa0142400:CDS
CACGTTCGATACAGTAATGA+TGG 0.531854 1_4:+1306310 Msa0142400:CDS
GGAAGAAGATTCTTATTGGA+AGG 0.535054 1_4:+1305749 Msa0142400:CDS
GTGTAGAAACATGTTCTGAA+TGG 0.541579 1_4:-1305986 None:intergenic
CTTGATCGAAGATTTCGTCT+AGG 0.546863 1_4:-1305587 None:intergenic
GCAGCTGGTTCATGCAAGGA+GGG 0.553162 1_4:-1305440 None:intergenic
AGATCTTCTGCTTGGAGTGG+TGG 0.557382 1_4:-1305486 None:intergenic
TTGCTGCAGCAATTCTTGCA+TGG 0.560772 1_4:-1305616 None:intergenic
GATTCATGATATTGAAGTCG+AGG 0.561299 1_4:-1305647 None:intergenic
CATGAATCATGAACAAGCAA+TGG 0.561832 1_4:+1305662 Msa0142400:CDS
TCTGTGGAGTAGCCATAAGA+TGG 0.563300 1_4:+1306592 Msa0142400:CDS
TTGAAGGGGTATAGACGTGT+TGG 0.563890 1_4:+1306728 Msa0142400:CDS
ACTATCTACCATGAATGTGA+TGG 0.583158 1_4:+1305389 None:intergenic
TCTGCCTGTTTGTTGAACTG+AGG 0.584240 1_4:-1306249 None:intergenic
TGTGATTGCTGTTACTCTAA+GGG 0.585072 1_4:-1306207 None:intergenic
GAGTACATGCAATATTTCAC+AGG 0.587078 1_4:+1306754 Msa0142400:CDS
ATTACTGTATCGAACGTGAA+CGG 0.593883 1_4:-1306305 None:intergenic
CAGTTCAACAAACAGGCAGA+AGG 0.599250 1_4:+1306252 Msa0142400:CDS
TCATGCAGCTGGTTCATGCA+AGG 0.599943 1_4:-1305444 None:intergenic
TGCAGCTGGTTCATGCAAGG+AGG 0.602202 1_4:-1305441 None:intergenic
ATTGCAAAGTCAACCATCAA+CGG 0.607337 1_4:-1306167 None:intergenic
CAGATTGAAACAGAAGTCAA+AGG 0.620209 1_4:-1306860 None:intergenic
ATGAGATCTTCTGCTTGGAG+TGG 0.651380 1_4:-1305489 None:intergenic
ATATCATGGAGGGAATCCAA+TGG 0.669550 1_4:+1306141 Msa0142400:CDS
CTAGAGAATGAATTGTGTGG+TGG 0.675354 1_4:+1305687 Msa0142400:CDS
CAGCTGGTTCATGCAAGGAG+GGG 0.677543 1_4:-1305439 None:intergenic
ATTCACTTGTAAGTACTCTG+TGG 0.679195 1_4:+1306576 Msa0142400:CDS
TCAATTGTATGATACATCAG+TGG 0.691913 1_4:+1306381 Msa0142400:CDS
GCAAAGTCAACCATCAACGG+TGG 0.711871 1_4:-1306164 None:intergenic
ACGTAACCAAATCATCACCA+AGG 0.735440 1_4:+1306447 Msa0142400:CDS
TAGAGTAACAGCAATCACAG+TGG 0.741841 1_4:+1306211 Msa0142400:CDS
GCTCTAGAGAATGAATTGTG+TGG 0.769887 1_4:+1305684 Msa0142400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATTTTTTTATTCATTGA+AGG + chr1_4:1306712-1306731 Msa0142400:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG + chr1_4:1306713-1306732 Msa0142400:CDS 15.0%
!!! AAGATTTTTTTATTCATTGA+AGG + chr1_4:1306712-1306731 Msa0142400:CDS 15.0%
!!! AGATTTTTTTATTCATTGAA+GGG + chr1_4:1306713-1306732 Msa0142400:CDS 15.0%
!!! AAATGAGCAAATTTTACATA+GGG - chr1_4:1306056-1306075 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG - chr1_4:1306057-1306076 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG + chr1_4:1306714-1306733 Msa0142400:CDS 20.0%
!! TTACTCATATTATAATCTGT+TGG - chr1_4:1306890-1306909 None:intergenic 20.0%
!!! AAATGAGCAAATTTTACATA+GGG - chr1_4:1306056-1306075 None:intergenic 20.0%
!!! AAAATGAGCAAATTTTACAT+AGG - chr1_4:1306057-1306076 None:intergenic 20.0%
!!! GATTTTTTTATTCATTGAAG+GGG + chr1_4:1306714-1306733 Msa0142400:CDS 20.0%
!! TTACTCATATTATAATCTGT+TGG - chr1_4:1306890-1306909 None:intergenic 20.0%
!! ATTTCATCTAGGATTGTATT+TGG - chr1_4:1305519-1305538 None:intergenic 25.0%
! GATCAAACAATATTTCATCT+AGG - chr1_4:1305530-1305549 None:intergenic 25.0%
!!! TGTCATTGATTTTGATATCA+TGG + chr1_4:1306127-1306146 Msa0142400:CDS 25.0%
!!! ATTGATTTTGATATCATGGA+GGG + chr1_4:1306131-1306150 Msa0142400:CDS 25.0%
! GAATTCAAAGAAATTGAACT+TGG + chr1_4:1306344-1306363 Msa0142400:CDS 25.0%
!! ATTTCATCTAGGATTGTATT+TGG - chr1_4:1305519-1305538 None:intergenic 25.0%
! GATCAAACAATATTTCATCT+AGG - chr1_4:1305530-1305549 None:intergenic 25.0%
!!! TGTCATTGATTTTGATATCA+TGG + chr1_4:1306127-1306146 Msa0142400:CDS 25.0%
!!! ATTGATTTTGATATCATGGA+GGG + chr1_4:1306131-1306150 Msa0142400:CDS 25.0%
! GAATTCAAAGAAATTGAACT+TGG + chr1_4:1306344-1306363 Msa0142400:CDS 25.0%
! ATTTCGTCTAGGATTGTATT+TGG - chr1_4:1305579-1305598 None:intergenic 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG + chr1_4:1305804-1305823 Msa0142400:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG + chr1_4:1306130-1306149 Msa0142400:CDS 30.0%
TCAATTGTATGATACATCAG+TGG + chr1_4:1306381-1306400 Msa0142400:CDS 30.0%
CAATACCTCTTTCTCTATAA+GGG - chr1_4:1306624-1306643 None:intergenic 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG + chr1_4:1306623-1306642 Msa0142400:CDS 30.0%
AAAAAGAGGAGAGAATATTG+TGG + chr1_4:1306684-1306703 Msa0142400:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG + chr1_4:1306685-1306704 Msa0142400:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG + chr1_4:1306862-1306881 Msa0142400:CDS 30.0%
! ATTTCGTCTAGGATTGTATT+TGG - chr1_4:1305579-1305598 None:intergenic 30.0%
! TTAGTTGATCTTTTGCTGAT+AGG + chr1_4:1305804-1305823 Msa0142400:CDS 30.0%
!!! CATTGATTTTGATATCATGG+AGG + chr1_4:1306130-1306149 Msa0142400:CDS 30.0%
TCAATTGTATGATACATCAG+TGG + chr1_4:1306381-1306400 Msa0142400:CDS 30.0%
CAATACCTCTTTCTCTATAA+GGG - chr1_4:1306624-1306643 None:intergenic 30.0%
!! CTTATAGAGAAAGAGGTATT+GGG + chr1_4:1306623-1306642 Msa0142400:CDS 30.0%
AAAAAGAGGAGAGAATATTG+TGG + chr1_4:1306684-1306703 Msa0142400:CDS 30.0%
AAAAGAGGAGAGAATATTGT+GGG + chr1_4:1306685-1306704 Msa0142400:CDS 30.0%
! TTTGACTTCTGTTTCAATCT+GGG + chr1_4:1306862-1306881 Msa0142400:CDS 30.0%
GAGATAAGAGATGTTGTGAT+TGG - chr1_4:1305557-1305576 None:intergenic 35.0%
GATTCATGATATTGAAGTCG+AGG - chr1_4:1305650-1305669 None:intergenic 35.0%
CATGAATCATGAACAAGCAA+TGG + chr1_4:1305662-1305681 Msa0142400:CDS 35.0%
! TGGAAACAAACACATTTTCG+CGG - chr1_4:1305728-1305747 None:intergenic 35.0%
CGAAAATGTGTTTGTTTCCA+GGG + chr1_4:1305728-1305747 Msa0142400:CDS 35.0%
GGAAGAAGATTCTTATTGGA+AGG + chr1_4:1305749-1305768 Msa0142400:CDS 35.0%
GGAAGTTCAAGAAGAGTTAA+TGG + chr1_4:1305770-1305789 Msa0142400:CDS 35.0%
AAGGCGATAGTTTATTGAAC+AGG + chr1_4:1305907-1305926 Msa0142400:CDS 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG + chr1_4:1305930-1305949 Msa0142400:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG - chr1_4:1305981-1306000 None:intergenic 35.0%
! GTGTAGAAACATGTTCTGAA+TGG - chr1_4:1305989-1306008 None:intergenic 35.0%
ATTGCAAAGTCAACCATCAA+CGG - chr1_4:1306170-1306189 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG - chr1_4:1306210-1306229 None:intergenic 35.0%
ATTACTGTATCGAACGTGAA+CGG - chr1_4:1306308-1306327 None:intergenic 35.0%
ATCAATGAGAAACTCCTGTT+TGG - chr1_4:1306410-1306429 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG + chr1_4:1306576-1306595 Msa0142400:CDS 35.0%
CCAATACCTCTTTCTCTATA+AGG - chr1_4:1306625-1306644 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG + chr1_4:1306622-1306641 Msa0142400:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG + chr1_4:1306670-1306689 Msa0142400:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG - chr1_4:1306680-1306699 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG + chr1_4:1306754-1306773 Msa0142400:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG + chr1_4:1306766-1306785 Msa0142400:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG + chr1_4:1306779-1306798 Msa0142400:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG + chr1_4:1306785-1306804 Msa0142400:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG + chr1_4:1306786-1306805 Msa0142400:CDS 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG + chr1_4:1306793-1306812 Msa0142400:CDS 35.0%
GCAATATGAGAACTGTAAGT+TGG + chr1_4:1306817-1306836 Msa0142400:CDS 35.0%
CAGATTGAAACAGAAGTCAA+AGG - chr1_4:1306863-1306882 None:intergenic 35.0%
! CTTTGACTTCTGTTTCAATC+TGG + chr1_4:1306861-1306880 Msa0142400:CDS 35.0%
GAGATAAGAGATGTTGTGAT+TGG - chr1_4:1305557-1305576 None:intergenic 35.0%
GATTCATGATATTGAAGTCG+AGG - chr1_4:1305650-1305669 None:intergenic 35.0%
CATGAATCATGAACAAGCAA+TGG + chr1_4:1305662-1305681 Msa0142400:CDS 35.0%
! TGGAAACAAACACATTTTCG+CGG - chr1_4:1305728-1305747 None:intergenic 35.0%
CGAAAATGTGTTTGTTTCCA+GGG + chr1_4:1305728-1305747 Msa0142400:CDS 35.0%
GGAAGAAGATTCTTATTGGA+AGG + chr1_4:1305749-1305768 Msa0142400:CDS 35.0%
GGAAGTTCAAGAAGAGTTAA+TGG + chr1_4:1305770-1305789 Msa0142400:CDS 35.0%
AAGGCGATAGTTTATTGAAC+AGG + chr1_4:1305907-1305926 Msa0142400:CDS 35.0%
! TTGTGTCTTTTCTTTACGCA+AGG + chr1_4:1305930-1305949 Msa0142400:CDS 35.0%
! ACATGTTCTGAATGGAACTT+AGG - chr1_4:1305981-1306000 None:intergenic 35.0%
! GTGTAGAAACATGTTCTGAA+TGG - chr1_4:1305989-1306008 None:intergenic 35.0%
ATTGCAAAGTCAACCATCAA+CGG - chr1_4:1306170-1306189 None:intergenic 35.0%
! TGTGATTGCTGTTACTCTAA+GGG - chr1_4:1306210-1306229 None:intergenic 35.0%
ATTACTGTATCGAACGTGAA+CGG - chr1_4:1306308-1306327 None:intergenic 35.0%
ATCAATGAGAAACTCCTGTT+TGG - chr1_4:1306410-1306429 None:intergenic 35.0%
ATTCACTTGTAAGTACTCTG+TGG + chr1_4:1306576-1306595 Msa0142400:CDS 35.0%
CCAATACCTCTTTCTCTATA+AGG - chr1_4:1306625-1306644 None:intergenic 35.0%
! CCTTATAGAGAAAGAGGTAT+TGG + chr1_4:1306622-1306641 Msa0142400:CDS 35.0%
ACAGTTTCCTTGTGAAAAAG+AGG + chr1_4:1306670-1306689 Msa0142400:CDS 35.0%
!! ATTCTCTCCTCTTTTTCACA+AGG - chr1_4:1306680-1306699 None:intergenic 35.0%
GAGTACATGCAATATTTCAC+AGG + chr1_4:1306754-1306773 Msa0142400:CDS 35.0%
TATTTCACAGGCTAAGCTAT+TGG + chr1_4:1306766-1306785 Msa0142400:CDS 35.0%
!! AAGCTATTGGTTAGCTTGTT+TGG + chr1_4:1306779-1306798 Msa0142400:CDS 35.0%
!! TTGGTTAGCTTGTTTGGTAA+AGG + chr1_4:1306785-1306804 Msa0142400:CDS 35.0%
!! TGGTTAGCTTGTTTGGTAAA+GGG + chr1_4:1306786-1306805 Msa0142400:CDS 35.0%
!! CTTGTTTGGTAAAGGGTATT+GGG + chr1_4:1306793-1306812 Msa0142400:CDS 35.0%
GCAATATGAGAACTGTAAGT+TGG + chr1_4:1306817-1306836 Msa0142400:CDS 35.0%
CAGATTGAAACAGAAGTCAA+AGG - chr1_4:1306863-1306882 None:intergenic 35.0%
! CTTTGACTTCTGTTTCAATC+TGG + chr1_4:1306861-1306880 Msa0142400:CDS 35.0%
CTTGATCGAAGATTTCGTCT+AGG - chr1_4:1305590-1305609 None:intergenic 40.0%
!! GCTCTAGAGAATGAATTGTG+TGG + chr1_4:1305684-1305703 Msa0142400:CDS 40.0%
CTAGAGAATGAATTGTGTGG+TGG + chr1_4:1305687-1305706 Msa0142400:CDS 40.0%
GCGAAAATGTGTTTGTTTCC+AGG + chr1_4:1305727-1305746 Msa0142400:CDS 40.0%
CCAATAAGAATCTTCTTCCC+TGG - chr1_4:1305748-1305767 None:intergenic 40.0%
CCAGGGAAGAAGATTCTTAT+TGG + chr1_4:1305745-1305764 Msa0142400:CDS 40.0%
CTAAACAATGCCTCGTCTTT+AGG + chr1_4:1305882-1305901 Msa0142400:CDS 40.0%
! AATGCCTCGTCTTTAGGAAA+AGG + chr1_4:1305888-1305907 Msa0142400:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG - chr1_4:1306084-1306103 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG + chr1_4:1306141-1306160 Msa0142400:CDS 40.0%
! CTGTGATTGCTGTTACTCTA+AGG - chr1_4:1306211-1306230 None:intergenic 40.0%
TAGAGTAACAGCAATCACAG+TGG + chr1_4:1306211-1306230 Msa0142400:CDS 40.0%
CACGTTCGATACAGTAATGA+TGG + chr1_4:1306310-1306329 Msa0142400:CDS 40.0%
ACGTAACCAAATCATCACCA+AGG + chr1_4:1306447-1306466 Msa0142400:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG - chr1_4:1306456-1306475 None:intergenic 40.0%
TCGACTAAAACAACAAGCCT+TGG - chr1_4:1306467-1306486 None:intergenic 40.0%
ACTCAAATCCATGTCCTTTG+TGG + chr1_4:1306511-1306530 Msa0142400:CDS 40.0%
GAAAAACTCCACAAAGGACA+TGG - chr1_4:1306522-1306541 None:intergenic 40.0%
CTCACAGAAAAACTCCACAA+AGG - chr1_4:1306528-1306547 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG + chr1_4:1306526-1306545 Msa0142400:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG - chr1_4:1306607-1306626 None:intergenic 40.0%
GTTAGCCCTTATAGAGAAAG+AGG + chr1_4:1306616-1306635 Msa0142400:CDS 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG + chr1_4:1306792-1306811 Msa0142400:CDS 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG + chr1_4:1306828-1306847 Msa0142400:CDS 40.0%
CTTGATCGAAGATTTCGTCT+AGG - chr1_4:1305590-1305609 None:intergenic 40.0%
!! GCTCTAGAGAATGAATTGTG+TGG + chr1_4:1305684-1305703 Msa0142400:CDS 40.0%
CTAGAGAATGAATTGTGTGG+TGG + chr1_4:1305687-1305706 Msa0142400:CDS 40.0%
GCGAAAATGTGTTTGTTTCC+AGG + chr1_4:1305727-1305746 Msa0142400:CDS 40.0%
CCAATAAGAATCTTCTTCCC+TGG - chr1_4:1305748-1305767 None:intergenic 40.0%
CCAGGGAAGAAGATTCTTAT+TGG + chr1_4:1305745-1305764 Msa0142400:CDS 40.0%
CTAAACAATGCCTCGTCTTT+AGG + chr1_4:1305882-1305901 Msa0142400:CDS 40.0%
! AATGCCTCGTCTTTAGGAAA+AGG + chr1_4:1305888-1305907 Msa0142400:CDS 40.0%
AGCTTCGAAAATCGCTTGAT+TGG - chr1_4:1306084-1306103 None:intergenic 40.0%
ATATCATGGAGGGAATCCAA+TGG + chr1_4:1306141-1306160 Msa0142400:CDS 40.0%
! CTGTGATTGCTGTTACTCTA+AGG - chr1_4:1306211-1306230 None:intergenic 40.0%
TAGAGTAACAGCAATCACAG+TGG + chr1_4:1306211-1306230 Msa0142400:CDS 40.0%
CACGTTCGATACAGTAATGA+TGG + chr1_4:1306310-1306329 Msa0142400:CDS 40.0%
ACGTAACCAAATCATCACCA+AGG + chr1_4:1306447-1306466 Msa0142400:CDS 40.0%
!! AACAAGCCTTGGTGATGATT+TGG - chr1_4:1306456-1306475 None:intergenic 40.0%
TCGACTAAAACAACAAGCCT+TGG - chr1_4:1306467-1306486 None:intergenic 40.0%
ACTCAAATCCATGTCCTTTG+TGG + chr1_4:1306511-1306530 Msa0142400:CDS 40.0%
GAAAAACTCCACAAAGGACA+TGG - chr1_4:1306522-1306541 None:intergenic 40.0%
CTCACAGAAAAACTCCACAA+AGG - chr1_4:1306528-1306547 None:intergenic 40.0%
! CTTTGTGGAGTTTTTCTGTG+AGG + chr1_4:1306526-1306545 Msa0142400:CDS 40.0%
TAAGGGCTAACTCCATCTTA+TGG - chr1_4:1306607-1306626 None:intergenic 40.0%
GTTAGCCCTTATAGAGAAAG+AGG + chr1_4:1306616-1306635 Msa0142400:CDS 40.0%
! GCTTGTTTGGTAAAGGGTAT+TGG + chr1_4:1306792-1306811 Msa0142400:CDS 40.0%
!! ACTGTAAGTTGGCTTTGTGT+TGG + chr1_4:1306828-1306847 Msa0142400:CDS 40.0%
CTAAGCTACATTCATGCAGC+TGG - chr1_4:1305458-1305477 None:intergenic 45.0%
ATGAGATCTTCTGCTTGGAG+TGG - chr1_4:1305492-1305511 None:intergenic 45.0%
GAGACATGAGATCTTCTGCT+TGG - chr1_4:1305497-1305516 None:intergenic 45.0%
TTGCTGCAGCAATTCTTGCA+TGG - chr1_4:1305619-1305638 None:intergenic 45.0%
! TGTGGTGGAGTTACCGAAAA+AGG + chr1_4:1305702-1305721 Msa0142400:CDS 45.0%
! CACATTTTCGCGGCCTTTTT+CGG - chr1_4:1305718-1305737 None:intergenic 45.0%
! ATCGCCTTTTCCTAAAGACG+AGG - chr1_4:1305895-1305914 None:intergenic 45.0%
GAAGCCTCAGTTCAACAAAC+AGG + chr1_4:1306245-1306264 Msa0142400:CDS 45.0%
! TCTGCCTGTTTGTTGAACTG+AGG - chr1_4:1306252-1306271 None:intergenic 45.0%
CAGTTCAACAAACAGGCAGA+AGG + chr1_4:1306252-1306271 Msa0142400:CDS 45.0%
TACATCAGTGGATGCCAAAC+AGG + chr1_4:1306393-1306412 Msa0142400:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG + chr1_4:1306592-1306611 Msa0142400:CDS 45.0%
! TTGAAGGGGTATAGACGTGT+TGG + chr1_4:1306728-1306747 Msa0142400:CDS 45.0%
CTAAGCTACATTCATGCAGC+TGG - chr1_4:1305458-1305477 None:intergenic 45.0%
ATGAGATCTTCTGCTTGGAG+TGG - chr1_4:1305492-1305511 None:intergenic 45.0%
GAGACATGAGATCTTCTGCT+TGG - chr1_4:1305497-1305516 None:intergenic 45.0%
TTGCTGCAGCAATTCTTGCA+TGG - chr1_4:1305619-1305638 None:intergenic 45.0%
! TGTGGTGGAGTTACCGAAAA+AGG + chr1_4:1305702-1305721 Msa0142400:CDS 45.0%
! CACATTTTCGCGGCCTTTTT+CGG - chr1_4:1305718-1305737 None:intergenic 45.0%
! ATCGCCTTTTCCTAAAGACG+AGG - chr1_4:1305895-1305914 None:intergenic 45.0%
GAAGCCTCAGTTCAACAAAC+AGG + chr1_4:1306245-1306264 Msa0142400:CDS 45.0%
! TCTGCCTGTTTGTTGAACTG+AGG - chr1_4:1306252-1306271 None:intergenic 45.0%
CAGTTCAACAAACAGGCAGA+AGG + chr1_4:1306252-1306271 Msa0142400:CDS 45.0%
TACATCAGTGGATGCCAAAC+AGG + chr1_4:1306393-1306412 Msa0142400:CDS 45.0%
TCTGTGGAGTAGCCATAAGA+TGG + chr1_4:1306592-1306611 Msa0142400:CDS 45.0%
! TTGAAGGGGTATAGACGTGT+TGG + chr1_4:1306728-1306747 Msa0142400:CDS 45.0%
! TCATGCAGCTGGTTCATGCA+AGG - chr1_4:1305447-1305466 None:intergenic 50.0%
AGATCTTCTGCTTGGAGTGG+TGG - chr1_4:1305489-1305508 None:intergenic 50.0%
! GCGATTTTCGAAGCTACAGC+TGG + chr1_4:1306089-1306108 Msa0142400:CDS 50.0%
AATCCAATGGCCACCGTTGA+TGG + chr1_4:1306154-1306173 Msa0142400:CDS 50.0%
GCAAAGTCAACCATCAACGG+TGG - chr1_4:1306167-1306186 None:intergenic 50.0%
! TCATGCAGCTGGTTCATGCA+AGG - chr1_4:1305447-1305466 None:intergenic 50.0%
AGATCTTCTGCTTGGAGTGG+TGG - chr1_4:1305489-1305508 None:intergenic 50.0%
! GCGATTTTCGAAGCTACAGC+TGG + chr1_4:1306089-1306108 Msa0142400:CDS 50.0%
AATCCAATGGCCACCGTTGA+TGG + chr1_4:1306154-1306173 Msa0142400:CDS 50.0%
GCAAAGTCAACCATCAACGG+TGG - chr1_4:1306167-1306186 None:intergenic 50.0%
! CAGCTGGTTCATGCAAGGAG+GGG - chr1_4:1305442-1305461 None:intergenic 55.0%
! GCAGCTGGTTCATGCAAGGA+GGG - chr1_4:1305443-1305462 None:intergenic 55.0%
! TGCAGCTGGTTCATGCAAGG+AGG - chr1_4:1305444-1305463 None:intergenic 55.0%
CAACCATCAACGGTGGCCAT+TGG - chr1_4:1306160-1306179 None:intergenic 55.0%
! CAGCTGGTTCATGCAAGGAG+GGG - chr1_4:1305442-1305461 None:intergenic 55.0%
! GCAGCTGGTTCATGCAAGGA+GGG - chr1_4:1305443-1305462 None:intergenic 55.0%
! TGCAGCTGGTTCATGCAAGG+AGG - chr1_4:1305444-1305463 None:intergenic 55.0%
CAACCATCAACGGTGGCCAT+TGG - chr1_4:1306160-1306179 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1305399 1306922 1305399 ID=Msa0142400;Name=Msa0142400
chr1_4 mRNA 1305399 1306922 1305399 ID=Msa0142400-mRNA-1;Parent=Msa0142400;Name=Msa0142400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|507
chr1_4 exon 1305399 1306922 1305399 ID=Msa0142400-mRNA-1:exon:428;Parent=Msa0142400-mRNA-1
chr1_4 CDS 1305399 1306922 1305399 ID=Msa0142400-mRNA-1:cds;Parent=Msa0142400-mRNA-1
Gene Sequence

>Msa0142400

ATGAATGTGATGGATTATGAGCAATTTGATTACTCATTCACCCCTCCTTGCATGAACCAGCTGCATGAATGTAGCTTAGAAAATTTACCACCACTCCAAGCAGAAGATCTCATGTCTCCAAATACAATCCTAGATGAAATATTGTTTGATCAAGACCAATCACAACATCTCTTATCTCCAAATACAATCCTAGACGAAATCTTCGATCAAGAAGAGTCCATGCAAGAATTGCTGCAGCAACATACAAACCTCGACTTCAATATCATGAATCATGAACAAGCAATGGCTCTAGAGAATGAATTGTGTGGTGGAGTTACCGAAAAAGGCCGCGAAAATGTGTTTGTTTCCAGGGAAGAAGATTCTTATTGGAAGGAAGTTCAAGAAGAGTTAATGGAAGAGACAAGTTTAGTTGATCTTTTGCTGATAGGAGCTGAAGCTGCTGAATCACAAAACATGACTCTTGCTTCTGACATAATCGAAAAACTAAACAATGCCTCGTCTTTAGGAAAAGGCGATAGTTTATTGAACAGGTTGTGTCTTTTCTTTACGCAAGGATTGTATTATAAAACTACAAATGCTCCTAAGTTCCATTCAGAACATGTTTCTACACAGACAAGCACATTCTGTGTGTTTCAGATACTTCAAGAACTCTCTCCCTATGTAAAATTTGCTCATTTTACTGCCAATCAAGCGATTTTCGAAGCTACAGCTGGTGTCGAAGATGTTCATGTCATTGATTTTGATATCATGGAGGGAATCCAATGGCCACCGTTGATGGTTGACTTTGCAATGAGAAAGAAGACTACTTCCCTTAGAGTAACAGCAATCACAGTGGATCAGCAAAGTGAAGCCTCAGTTCAACAAACAGGCAGAAGGCTCAAAGAGTTCGCTGATTCAATCAATTTTCCGTTCACGTTCGATACAGTAATGATGGTGAGTGAAGAAGAATTCAAAGAAATTGAACTTGGTGAAACATTTATTGTCAATTGTATGATACATCAGTGGATGCCAAACAGGAGTTTCTCATTGATCAAAGCTTTTCTCGATCACGTAACCAAATCATCACCAAGGCTTGTTGTTTTAGTCGAAGAAGAACTGTTTAATTTTTCTAGACTCAAATCCATGTCCTTTGTGGAGTTTTTCTGTGAGGCTTTGCATCACTATATTGCAGTTTCTGATTCACTTGTAAGTACTCTGTGGAGTAGCCATAAGATGGAGTTAGCCCTTATAGAGAAAGAGGTATTGGGAAATAGAATCTTAGACAGTGTAAGACAGTTTCCTTGTGAAAAAGAGGAGAGAATATTGTGGGAAGAAAGATTTTTTTATTCATTGAAGGGGTATAGACGTGTTGGTATGAGTACATGCAATATTTCACAGGCTAAGCTATTGGTTAGCTTGTTTGGTAAAGGGTATTGGGTGCAATATGAGAACTGTAAGTTGGCTTTGTGTTGGAAGTCAAGACCTTTGACTTCTGTTTCAATCTGGGTACCAACAGATTATAATATGAGTAACAAAGTCAAATAG

Protein sequence

>Msa0142400

MNVMDYEQFDYSFTPPCMNQLHECSLENLPPLQAEDLMSPNTILDEILFDQDQSQHLLSPNTILDEIFDQEESMQELLQQHTNLDFNIMNHEQAMALENELCGGVTEKGRENVFVSREEDSYWKEVQEELMEETSLVDLLLIGAEAAESQNMTLASDIIEKLNNASSLGKGDSLLNRLCLFFTQGLYYKTTNAPKFHSEHVSTQTSTFCVFQILQELSPYVKFAHFTANQAIFEATAGVEDVHVIDFDIMEGIQWPPLMVDFAMRKKTTSLRVTAITVDQQSEASVQQTGRRLKEFADSINFPFTFDTVMMVSEEEFKEIELGETFIVNCMIHQWMPNRSFSLIKAFLDHVTKSSPRLVVLVEEELFNFSRLKSMSFVEFFCEALHHYIAVSDSLVSTLWSSHKMELALIEKEVLGNRILDSVRQFPCEKEERILWEERFFYSLKGYRRVGMSTCNISQAKLLVSLFGKGYWVQYENCKLALCWKSRPLTSVSIWVPTDYNMSNKVK*