AlfalfaGEDB | Msa0157060

M. sativa cultivar zhongmu-4 / Msa0157060

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0157060	XP_003590181.2	97.222	252	7	0	1	252	7	258	0.0	513

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0157060	sp\|Q10MB4\|MYB2_ORYSJ	72.807	114	27	1	11	120	12	125	9.63e-54	179

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0157060	G7I6P8	97.222	252	7	0	1	252	7	258	0.0	513

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TFs/TRs:

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Gene ID	Type	Classification
Msa0157060	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017150	Msa0157060	0.808039	3.766230e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0157060	MtrunA17_Chr1g0168661	97.222	252	7	0	1	252	7	258	0.0	513
Msa0157060	MtrunA17_Chr1g0186061	66.946	239	71	4	1	232	6	243	2.46e-101	295
Msa0157060	MtrunA17_Chr1g0209661	63.710	124	43	1	11	134	23	144	1.18e-53	176
Msa0157060	MtrunA17_Chr7g0228231	67.273	110	36	0	16	125	15	124	7.13e-53	170
Msa0157060	MtrunA17_Chr1g0194301	69.159	107	33	0	14	120	32	138	2.20e-52	171
Msa0157060	MtrunA17_Chr6g0450101	68.519	108	34	0	16	123	15	122	2.53e-52	169
Msa0157060	MtrunA17_Chr1g0194291	70.476	105	31	0	16	120	22	126	3.26e-52	170
Msa0157060	MtrunA17_Chr2g0327821	64.602	113	39	1	9	121	1	112	2.48e-50	164
Msa0157060	MtrunA17_Chr4g0030881	62.393	117	37	1	9	118	1	117	2.42e-49	163
Msa0157060	MtrunA17_Chr4g0039971	60.909	110	43	0	9	118	7	116	8.48e-47	159

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0157060	AT4G13480.1	56.180	267	87	8	10	252	1	261	6.59e-87	259
Msa0157060	AT3G24310.1	59.231	260	82	6	12	252	15	269	9.14e-87	259
Msa0157060	AT3G30210.1	47.059	255	94	8	16	252	28	259	3.85e-64	201
Msa0157060	AT3G46130.1	39.844	256	131	6	9	252	3	247	1.01e-53	174
Msa0157060	AT5G59780.3	69.725	109	33	0	12	120	5	113	2.53e-53	172
Msa0157060	AT3G27810.1	68.142	113	35	1	11	123	17	128	5.31e-53	171
Msa0157060	AT5G40350.1	42.396	217	105	5	16	232	18	214	1.12e-52	170
Msa0157060	AT1G48000.1	71.429	105	30	0	16	120	33	137	7.44e-52	169
Msa0157060	AT1G68320.1	66.972	109	36	0	12	120	16	124	2.61e-51	169
Msa0157060	AT1G25340.1	67.619	105	34	0	16	120	19	123	1.98e-50	167

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Find 61 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGTTGCAGATTGAGGT+GGG	0.541748	1_4:-23983451	Msa0157060:CDS
AACAGAAGATTATAGTTTGT+TGG	0.401703	1_4:-23982690	Msa0157060:CDS
AACTCAGTTGCTAGGCTTAC+AGG	0.272684	1_4:-23983881	Msa0157060:intron
AATATTATGCTTTCTTCTTG+TGG	0.423745	1_4:+23983387	None:intergenic
AATTGGTCATGGTATCATGG+AGG	0.749718	1_4:-23983990	Msa0157060:CDS
ACAACAACTGCAATTCAACT+TGG	0.507410	1_4:-23982867	Msa0157060:CDS
ACAATGAAATAAAGAATTAT+TGG	0.288046	1_4:-23983018	Msa0157060:CDS
ACAATTGCAAGAAGTTTACC+GGG	0.627274	1_4:-23983049	Msa0157060:CDS
AGGACCATGGACTGCTGAAG+AGG	0.572659	1_4:-23983951	Msa0157060:CDS
AGGTGTCATGTCCATGTTTG+AGG	0.551244	1_4:-23982837	Msa0157060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAACTTATCTACTATAGC+CGG	+	chr1_4:23982967-23982986	None:intergenic	30.0%
TAACAACTTGATCATAGTTG+AGG	+	chr1_4:23983073-23983092	None:intergenic	30.0%
TCTTGTGAATAGGATTGAAA+AGG	-	chr1_4:23983114-23983133	Msa0157060:intron	30.0%
TGAATAGGATTGAAAAGGAA+TGG	-	chr1_4:23983119-23983138	Msa0157060:intron	30.0%
TAATATTAGAGCTTCATGCT+AGG	-	chr1_4:23983222-23983241	Msa0157060:intron	30.0%
CATGTTGAAAATTTCTTGTC+TGG	+	chr1_4:23983818-23983837	None:intergenic	30.0%
CTATAATCTTCTGTTGCATT+AGG	+	chr1_4:23983898-23983917	None:intergenic	30.0%
TGGAATTTGGATGATATTCA+AGG	-	chr1_4:23983934-23983953	Msa0157060:CDS	30.0%
TGAAACTTATCTACTATAGC+CGG	+	chr1_4:23982967-23982986	None:intergenic	30.0%
TAACAACTTGATCATAGTTG+AGG	+	chr1_4:23983073-23983092	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	23982592	23984023	23982592	ID=Msa0157060;Name=Msa0157060
chr1_4	mRNA	23982592	23984023	23982592	ID=Msa0157060-mRNA-1;Parent=Msa0157060;Name=Msa0157060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|3\|0\|252
chr1_4	exon	23983882	23984023	23983882	ID=Msa0157060-mRNA-1:exon:8324;Parent=Msa0157060-mRNA-1
chr1_4	exon	23983360	23983489	23983360	ID=Msa0157060-mRNA-1:exon:8323;Parent=Msa0157060-mRNA-1
chr1_4	exon	23982592	23983078	23982592	ID=Msa0157060-mRNA-1:exon:8322;Parent=Msa0157060-mRNA-1
chr1_4	CDS	23983882	23984023	23983882	ID=Msa0157060-mRNA-1:cds;Parent=Msa0157060-mRNA-1
chr1_4	CDS	23983360	23983489	23983360	ID=Msa0157060-mRNA-1:cds;Parent=Msa0157060-mRNA-1
chr1_4	CDS	23982592	23983078	23982592	ID=Msa0157060-mRNA-1:cds;Parent=Msa0157060-mRNA-1

Gene Sequence

>Msa0157060

ATGGCAGGCCAAATTGGTCATGGTATCATGGAGGAAGAGGTTTGGAGGAAAGGACCATGGACTGCTGAAGAGGATAGGTTGTTGATTGACTATGTTAGGCTGCATGGTGAAGGCAGATGGAACTCAGTTGCTAGGCTTACAGGATTGAAAAGGAATGGAAAGAGTTGCAGATTGAGGTGGGTTAATTATCTAAGACCAGATCTCAAAAGAGGTCAAATAACACCACAAGAAGAAAGCATAATATTAGAGCTTCATGCTAGGTGGGGAAACAGATGGTCCACAATTGCAAGAAGTTTACCGGGAAGAACAGACAATGAAATAAAGAATTATTGGAGGACTCATTTCAAGAAAAAAGCAAAAAATCCCGATGATGCTGCCGAAAAGGCAAAAAACCGATTCCTGAGAAAGCAGTTATTTCACCAGCAGCAACAACAACAACAATTGCAGCAGCAGCAGCAACAACAACAACTGCAATTCAACTTGGACATGAAAGGTGTCATGTCCATGTTTGAGGAAAACAATAGCCATAGAATTCCTTATATATCTCAAACCAGACAAGAAATTTTCAACATGTATCCAAATACAACAGAGGATCAGGGTTACGTTATGCTCAATGGAAACTCTTCTGTCCCTAATGCAACAGAAGATTATAGTTTGTTGGATGGTCTGTGGAATTTGGATGATATTCAAGGAAATTTCAGTGTTGCTAATGCAACAAACAAAGCTGGTCTATACAATTTAGTTGCTCCTTACTGTTAG

Protein sequence

>Msa0157060

MAGQIGHGIMEEEVWRKGPWTAEEDRLLIDYVRLHGEGRWNSVARLTGLKRNGKSCRLRWVNYLRPDLKRGQITPQEESIILELHARWGNRWSTIARSLPGRTDNEIKNYWRTHFKKKAKNPDDAAEKAKNRFLRKQLFHQQQQQQQLQQQQQQQQLQFNLDMKGVMSMFEENNSHRIPYISQTRQEIFNMYPNTTEDQGYVMLNGNSSVPNATEDYSLLDGLWNLDDIQGNFSVANATNKAGLYNLVAPYC*