AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0169600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0169600 MtrunA17_Chr1g0186341 93.645 299 18 1 1 299 1 298 1.39e-170 474
Msa0169600 MtrunA17_Chr1g0183391 26.667 345 182 14 1 298 1 321 2.63e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0169600 AT2G33400.1 29.967 307 138 10 1 288 1 249 2.26e-25 102
Msa0169600 AT5G44040.1 32.759 290 141 11 36 298 77 339 2.91e-18 84.7
Msa0169600 AT1G04030.2 28.086 324 173 14 1 285 1 303 1.05e-12 68.6
Msa0169600 AT1G04030.1 28.086 324 173 14 1 285 1 303 1.05e-12 68.6

Find 58 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAAGTTTGGAGAATTT+TGG 0.099083 1_4:-52786626 Msa0169600:CDS
AAAGAAAGAAAGAAAGAAAA+TGG 0.158174 1_4:-52787294 Msa0169600:exon
CCCTAGTTCTTATCAGGTTT+TGG 0.222472 1_4:-52786855 Msa0169600:CDS
ACAAACCTGATTTGAGAATA+TGG 0.294465 1_4:+52787058 None:intergenic
GGCAAACCCTAGTTCTTATC+AGG 0.299458 1_4:-52786861 Msa0169600:CDS
TTTCTTTCTTTCTCTTTCAA+TGG 0.327083 1_4:+52787306 None:intergenic
TTGTACCATATTCTCAAATC+AGG 0.328302 1_4:-52787063 Msa0169600:intron
TGATGAGGAAGATGAAAATA+AGG 0.334449 1_4:-52786825 Msa0169600:CDS
TGATTGGGATGATGAAAGTT+TGG 0.346270 1_4:-52786636 Msa0169600:CDS
TTCATTCATTAAAAGTTTGT+TGG 0.384333 1_4:-52786121 Msa0169600:three_prime_UTR
TCATTCATTAAAAGTTTGTT+GGG 0.396716 1_4:-52786120 Msa0169600:three_prime_UTR
GGAGGATGAAATAGTGTATG+AGG 0.408167 1_4:-52786705 Msa0169600:CDS
AAGAAAGAAAGAAAGAAAAT+GGG 0.409644 1_4:-52787293 Msa0169600:exon
GAAGTTCTCGCCTTGCGTCT+TGG 0.432670 1_4:-52786342 Msa0169600:CDS
TCAGTCGAAGCCTTTGTTGC+AGG 0.437491 1_4:-52786306 Msa0169600:CDS
AATTTGATGAAGGGTATGAT+TGG 0.441432 1_4:-52786652 Msa0169600:CDS
TGCTAGTCTTTCAAACTGGT+TGG 0.449139 1_4:-52786270 Msa0169600:CDS
AAGCATGCTGCCTGTAGTCT+TGG 0.451334 1_4:-52786375 Msa0169600:CDS
TTTGAATGTAGAGATGTTTG+AGG 0.463362 1_4:-52786882 Msa0169600:CDS
CAACAAAGGCTTCGACTGAA+TGG 0.464145 1_4:+52786310 None:intergenic
TTGATGCTAGTCTTTCAAAC+TGG 0.480004 1_4:-52786274 Msa0169600:CDS
AACAGTGAACAGAAAGAAGA+TGG 0.482964 1_4:-52786581 Msa0169600:CDS
ATGTTGCAGTATGCAATTTG+AGG 0.485238 1_4:-52785984 None:intergenic
GATGATGATGAATTTGATGA+AGG 0.486925 1_4:-52786662 Msa0169600:CDS
TAGAGGTAGAGATCAAGAAA+AGG 0.488908 1_4:-52786951 Msa0169600:CDS
CATTGAGTAAGATTCTCTAC+CGG 0.490839 1_4:+52786470 None:intergenic
CGGTAGAGAATCTTACTCAA+TGG 0.500520 1_4:-52786469 Msa0169600:CDS
ACTGCAACTTCCTGCAACAA+AGG 0.507022 1_4:+52786296 None:intergenic
TGTCTAAAACAGAAACTCGT+TGG 0.507304 1_4:-52787467 Msa0169600:five_prime_UTR
ACTCTCTACAAAGTCCTAGC+TGG 0.507971 1_4:-52787221 Msa0169600:CDS
GAGCTTGATTCCAAGACTAC+AGG 0.512568 1_4:+52786365 None:intergenic
ATAAGAAAGAATGTTTATGT+TGG 0.519157 1_4:+52787341 None:intergenic
CATGAATTCCGTTCTGCACC+CGG 0.525680 1_4:-52786489 Msa0169600:CDS
TCCAAAACCTGATAAGAACT+AGG 0.528369 1_4:+52786854 None:intergenic
ATCAAGCAAACAAACGAGAA+AGG 0.534474 1_4:-52786423 Msa0169600:CDS
ATTCGACGGGTATCGAATCA+TGG 0.538164 1_4:+52786763 None:intergenic
TTATGATATCTATGATTCGA+CGG 0.557585 1_4:+52786749 None:intergenic
CCAAAACCTGATAAGAACTA+GGG 0.562445 1_4:+52786855 None:intergenic
AGAAACTCGTTGGAGCCAAA+CGG 0.563148 1_4:-52787457 Msa0169600:five_prime_UTR
GATCAAGAAAAGGAGAAGAG+TGG 0.564885 1_4:-52786941 Msa0169600:CDS
TATGATATCTATGATTCGAC+GGG 0.566426 1_4:+52786750 None:intergenic
TTTCTGTTTCTTCAAGCACT+CGG 0.567496 1_4:+52786198 None:intergenic
TAGTTATGACAATGAGGAGG+AGG 0.567704 1_4:-52786723 Msa0169600:CDS
ATTTGATGAAGGGTATGATT+GGG 0.573696 1_4:-52786651 Msa0169600:CDS
ATGATGATGAATTTGATGAA+GGG 0.582122 1_4:-52786661 Msa0169600:CDS
ATTGAGTAAGATTCTCTACC+GGG 0.594847 1_4:+52786471 None:intergenic
CGAATTTGAGTGGACGAGAG+AGG 0.606574 1_4:-52786520 Msa0169600:CDS
ATTCTCTACCGGGTGCAGAA+CGG 0.617190 1_4:+52786481 None:intergenic
GAATTGAAATCGAATTTGAG+TGG 0.620274 1_4:-52786530 Msa0169600:CDS
TCATTGACAACGAATTGTAG+TGG 0.620784 1_4:+52786226 None:intergenic
ACAATTCGTTGTCAATGATG+TGG 0.639822 1_4:-52786221 Msa0169600:exon
ATCGAATTGTGATGCTTCTG+TGG 0.643973 1_4:-52786156 Msa0169600:three_prime_UTR
TGAAAATAAGGAGACTACTG+TGG 0.644236 1_4:-52786813 Msa0169600:CDS
TAACTGTAGTTATGACAATG+AGG 0.650246 1_4:-52786729 Msa0169600:CDS
GCATTCGATTCCAAGACGCA+AGG 0.653152 1_4:+52786332 None:intergenic
GTAGTGGAGACATTATAACT+AGG 0.665616 1_4:+52786242 None:intergenic
ACTGTGGAATCAGAGAGAGA+AGG 0.685984 1_4:-52786797 Msa0169600:CDS
CTGTAGTTATGACAATGAGG+AGG 0.688122 1_4:-52786726 Msa0169600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAATTTATTTGTTAG+AGG - chr1_4:52786496-52786515 Msa0169600:CDS 10.0%
!!! TTTTTTAATTTATTTGTTAG+AGG - chr1_4:52786496-52786515 Msa0169600:CDS 10.0%
!! ATAAGAAAGAATGTTTATGT+TGG + chr1_4:52786126-52786145 None:intergenic 20.0%
!! AAAGAAAGAAAGAAAGAAAA+TGG - chr1_4:52786170-52786189 Msa0169600:three_prime_UTR 20.0%
!! AAGAAAGAAAGAAAGAAAAT+GGG - chr1_4:52786171-52786190 Msa0169600:three_prime_UTR 20.0%
!! TGAAGAAATATTATTGTATG+TGG - chr1_4:52786448-52786467 Msa0169600:CDS 20.0%
!! TTCATTCATTAAAAGTTTGT+TGG - chr1_4:52787343-52787362 Msa0169600:five_prime_UTR 20.0%
!!! TCATTCATTAAAAGTTTGTT+GGG - chr1_4:52787344-52787363 Msa0169600:five_prime_UTR 20.0%
!! ATAAGAAAGAATGTTTATGT+TGG + chr1_4:52786126-52786145 None:intergenic 20.0%
!! AAAGAAAGAAAGAAAGAAAA+TGG - chr1_4:52786170-52786189 Msa0169600:three_prime_UTR 20.0%
!! AAGAAAGAAAGAAAGAAAAT+GGG - chr1_4:52786171-52786190 Msa0169600:three_prime_UTR 20.0%
!! TGAAGAAATATTATTGTATG+TGG - chr1_4:52786448-52786467 Msa0169600:CDS 20.0%
!! TTCATTCATTAAAAGTTTGT+TGG - chr1_4:52787343-52787362 Msa0169600:five_prime_UTR 20.0%
!!! TCATTCATTAAAAGTTTGTT+GGG - chr1_4:52787344-52787363 Msa0169600:five_prime_UTR 20.0%
! TTTCTTTCTTTCTCTTTCAA+TGG + chr1_4:52786161-52786180 None:intergenic 25.0%
!!! GTTGTTTTGATATACTTACT+TGG + chr1_4:52786275-52786294 None:intergenic 25.0%
! ATCAAGAACTTAAGAATGAT+GGG + chr1_4:52786297-52786316 None:intergenic 25.0%
! AATCAAGAACTTAAGAATGA+TGG + chr1_4:52786298-52786317 None:intergenic 25.0%
!! CTTATCTTTTTGCAGAAATA+TGG - chr1_4:52786340-52786359 Msa0169600:CDS 25.0%
! TTATGATATCTATGATTCGA+CGG + chr1_4:52786718-52786737 None:intergenic 25.0%
!! ATGATGATGAATTTGATGAA+GGG - chr1_4:52786803-52786822 Msa0169600:CDS 25.0%
!! ATGAAAGTTTGGAGAATTTT+GGG - chr1_4:52786839-52786858 Msa0169600:CDS 25.0%
!!! TCCTGTAATTGTTTTGTTTT+TGG - chr1_4:52787400-52787419 Msa0169600:five_prime_UTR 25.0%
! ACCAAAAACAAAACAATTAC+AGG + chr1_4:52787404-52787423 None:intergenic 25.0%
!!! TTTTGTTTTTGGTTTGTGAA+TGG - chr1_4:52787411-52787430 Msa0169600:five_prime_UTR 25.0%
! TTTCTTTCTTTCTCTTTCAA+TGG + chr1_4:52786161-52786180 None:intergenic 25.0%
!!! GTTGTTTTGATATACTTACT+TGG + chr1_4:52786275-52786294 None:intergenic 25.0%
! ATCAAGAACTTAAGAATGAT+GGG + chr1_4:52786297-52786316 None:intergenic 25.0%
! AATCAAGAACTTAAGAATGA+TGG + chr1_4:52786298-52786317 None:intergenic 25.0%
!! CTTATCTTTTTGCAGAAATA+TGG - chr1_4:52786340-52786359 Msa0169600:CDS 25.0%
! TTATGATATCTATGATTCGA+CGG + chr1_4:52786718-52786737 None:intergenic 25.0%
!! ATGATGATGAATTTGATGAA+GGG - chr1_4:52786803-52786822 Msa0169600:CDS 25.0%
!! ATGAAAGTTTGGAGAATTTT+GGG - chr1_4:52786839-52786858 Msa0169600:CDS 25.0%
!!! TCCTGTAATTGTTTTGTTTT+TGG - chr1_4:52787400-52787419 Msa0169600:five_prime_UTR 25.0%
! ACCAAAAACAAAACAATTAC+AGG + chr1_4:52787404-52787423 None:intergenic 25.0%
!!! TTTTGTTTTTGGTTTGTGAA+TGG - chr1_4:52787411-52787430 Msa0169600:five_prime_UTR 25.0%
TTGTACCATATTCTCAAATC+AGG - chr1_4:52786401-52786420 Msa0169600:CDS 30.0%
! ACAAACCTGATTTGAGAATA+TGG + chr1_4:52786409-52786428 None:intergenic 30.0%
TTTGAATGTAGAGATGTTTG+AGG - chr1_4:52786582-52786601 Msa0169600:CDS 30.0%
TGATGAGGAAGATGAAAATA+AGG - chr1_4:52786639-52786658 Msa0169600:CDS 30.0%
TATGATATCTATGATTCGAC+GGG + chr1_4:52786717-52786736 None:intergenic 30.0%
TAACTGTAGTTATGACAATG+AGG - chr1_4:52786735-52786754 Msa0169600:CDS 30.0%
! GATGATGATGAATTTGATGA+AGG - chr1_4:52786802-52786821 Msa0169600:CDS 30.0%
! AATTTGATGAAGGGTATGAT+TGG - chr1_4:52786812-52786831 Msa0169600:CDS 30.0%
! ATTTGATGAAGGGTATGATT+GGG - chr1_4:52786813-52786832 Msa0169600:CDS 30.0%
! GATGAAAGTTTGGAGAATTT+TGG - chr1_4:52786838-52786857 Msa0169600:CDS 30.0%
! TGAAAGTTTGGAGAATTTTG+GGG - chr1_4:52786840-52786859 Msa0169600:CDS 30.0%
GAATTGAAATCGAATTTGAG+TGG - chr1_4:52786934-52786953 Msa0169600:CDS 30.0%
TTGTACCATATTCTCAAATC+AGG - chr1_4:52786401-52786420 Msa0169600:CDS 30.0%
! ACAAACCTGATTTGAGAATA+TGG + chr1_4:52786409-52786428 None:intergenic 30.0%
TTTGAATGTAGAGATGTTTG+AGG - chr1_4:52786582-52786601 Msa0169600:CDS 30.0%
TGATGAGGAAGATGAAAATA+AGG - chr1_4:52786639-52786658 Msa0169600:CDS 30.0%
TATGATATCTATGATTCGAC+GGG + chr1_4:52786717-52786736 None:intergenic 30.0%
TAACTGTAGTTATGACAATG+AGG - chr1_4:52786735-52786754 Msa0169600:CDS 30.0%
! GATGATGATGAATTTGATGA+AGG - chr1_4:52786802-52786821 Msa0169600:CDS 30.0%
! AATTTGATGAAGGGTATGAT+TGG - chr1_4:52786812-52786831 Msa0169600:CDS 30.0%
! ATTTGATGAAGGGTATGATT+GGG - chr1_4:52786813-52786832 Msa0169600:CDS 30.0%
! GATGAAAGTTTGGAGAATTT+TGG - chr1_4:52786838-52786857 Msa0169600:CDS 30.0%
! TGAAAGTTTGGAGAATTTTG+GGG - chr1_4:52786840-52786859 Msa0169600:CDS 30.0%
GAATTGAAATCGAATTTGAG+TGG - chr1_4:52786934-52786953 Msa0169600:CDS 30.0%
! TGTCTAAAACAGAAACTCGT+TGG - chr1_4:52785997-52786016 Msa0169600:three_prime_UTR 35.0%
!!! GACAAAGTTTTAAAGCCGTT+TGG + chr1_4:52786025-52786044 None:intergenic 35.0%
!! GGTTGTTTTCTTCAGTGTTT+TGG - chr1_4:52786192-52786211 Msa0169600:three_prime_UTR 35.0%
ATTGTATGTGGATCTGTGAA+TGG - chr1_4:52786460-52786479 Msa0169600:CDS 35.0%
TAGAGGTAGAGATCAAGAAA+AGG - chr1_4:52786513-52786532 Msa0169600:CDS 35.0%
CCAAAACCTGATAAGAACTA+GGG + chr1_4:52786612-52786631 None:intergenic 35.0%
TCCAAAACCTGATAAGAACT+AGG + chr1_4:52786613-52786632 None:intergenic 35.0%
TGAAAATAAGGAGACTACTG+TGG - chr1_4:52786651-52786670 Msa0169600:CDS 35.0%
TGATTGGGATGATGAAAGTT+TGG - chr1_4:52786828-52786847 Msa0169600:CDS 35.0%
AACAGTGAACAGAAAGAAGA+TGG - chr1_4:52786883-52786902 Msa0169600:CDS 35.0%
ATTGAGTAAGATTCTCTACC+GGG + chr1_4:52786996-52787015 None:intergenic 35.0%
CATTGAGTAAGATTCTCTAC+CGG + chr1_4:52786997-52787016 None:intergenic 35.0%
ATCAAGCAAACAAACGAGAA+AGG - chr1_4:52787041-52787060 Msa0169600:intron 35.0%
!!! ATGCTTGTTTTTTGCTCTGA+TGG + chr1_4:52787075-52787094 None:intergenic 35.0%
!! TTGATGCTAGTCTTTCAAAC+TGG - chr1_4:52787190-52787209 Msa0169600:intron 35.0%
! GTAGTGGAGACATTATAACT+AGG + chr1_4:52787225-52787244 None:intergenic 35.0%
TCATTGACAACGAATTGTAG+TGG + chr1_4:52787241-52787260 None:intergenic 35.0%
ACAATTCGTTGTCAATGATG+TGG - chr1_4:52787243-52787262 Msa0169600:CDS 35.0%
! TTTCTGTTTCTTCAAGCACT+CGG + chr1_4:52787269-52787288 None:intergenic 35.0%
TCATGTTGCAGTATGCAATT+TGG - chr1_4:52787478-52787497 Msa0169600:five_prime_UTR 35.0%
! TGTCTAAAACAGAAACTCGT+TGG - chr1_4:52785997-52786016 Msa0169600:three_prime_UTR 35.0%
!!! GACAAAGTTTTAAAGCCGTT+TGG + chr1_4:52786025-52786044 None:intergenic 35.0%
!! GGTTGTTTTCTTCAGTGTTT+TGG - chr1_4:52786192-52786211 Msa0169600:three_prime_UTR 35.0%
ATTGTATGTGGATCTGTGAA+TGG - chr1_4:52786460-52786479 Msa0169600:CDS 35.0%
TAGAGGTAGAGATCAAGAAA+AGG - chr1_4:52786513-52786532 Msa0169600:CDS 35.0%
CCAAAACCTGATAAGAACTA+GGG + chr1_4:52786612-52786631 None:intergenic 35.0%
TCCAAAACCTGATAAGAACT+AGG + chr1_4:52786613-52786632 None:intergenic 35.0%
TGAAAATAAGGAGACTACTG+TGG - chr1_4:52786651-52786670 Msa0169600:CDS 35.0%
TGATTGGGATGATGAAAGTT+TGG - chr1_4:52786828-52786847 Msa0169600:CDS 35.0%
AACAGTGAACAGAAAGAAGA+TGG - chr1_4:52786883-52786902 Msa0169600:CDS 35.0%
ATTGAGTAAGATTCTCTACC+GGG + chr1_4:52786996-52787015 None:intergenic 35.0%
CATTGAGTAAGATTCTCTAC+CGG + chr1_4:52786997-52787016 None:intergenic 35.0%
ATCAAGCAAACAAACGAGAA+AGG - chr1_4:52787041-52787060 Msa0169600:intron 35.0%
!!! ATGCTTGTTTTTTGCTCTGA+TGG + chr1_4:52787075-52787094 None:intergenic 35.0%
!! TTGATGCTAGTCTTTCAAAC+TGG - chr1_4:52787190-52787209 Msa0169600:intron 35.0%
! GTAGTGGAGACATTATAACT+AGG + chr1_4:52787225-52787244 None:intergenic 35.0%
TCATTGACAACGAATTGTAG+TGG + chr1_4:52787241-52787260 None:intergenic 35.0%
ACAATTCGTTGTCAATGATG+TGG - chr1_4:52787243-52787262 Msa0169600:CDS 35.0%
! TTTCTGTTTCTTCAAGCACT+CGG + chr1_4:52787269-52787288 None:intergenic 35.0%
!! TTACTTGGTTTTCTCCAGCT+AGG + chr1_4:52786260-52786279 None:intergenic 40.0%
GATCAAGAAAAGGAGAAGAG+TGG - chr1_4:52786523-52786542 Msa0169600:CDS 40.0%
!! CCCTAGTTCTTATCAGGTTT+TGG - chr1_4:52786609-52786628 Msa0169600:CDS 40.0%
!!! GGTTTTGGATGATGATGATG+AGG - chr1_4:52786624-52786643 Msa0169600:CDS 40.0%
CTGTAGTTATGACAATGAGG+AGG - chr1_4:52786738-52786757 Msa0169600:CDS 40.0%
TAGTTATGACAATGAGGAGG+AGG - chr1_4:52786741-52786760 Msa0169600:CDS 40.0%
GGAGGATGAAATAGTGTATG+AGG - chr1_4:52786759-52786778 Msa0169600:CDS 40.0%
CGGTAGAGAATCTTACTCAA+TGG - chr1_4:52786995-52787014 Msa0169600:intron 40.0%
!! TGCTAGTCTTTCAAACTGGT+TGG - chr1_4:52787194-52787213 Msa0169600:intron 40.0%
ATCGAATTGTGATGCTTCTG+TGG - chr1_4:52787308-52787327 Msa0169600:five_prime_UTR 40.0%
!! TTACTTGGTTTTCTCCAGCT+AGG + chr1_4:52786260-52786279 None:intergenic 40.0%
GATCAAGAAAAGGAGAAGAG+TGG - chr1_4:52786523-52786542 Msa0169600:CDS 40.0%
!! CCCTAGTTCTTATCAGGTTT+TGG - chr1_4:52786609-52786628 Msa0169600:CDS 40.0%
!!! GGTTTTGGATGATGATGATG+AGG - chr1_4:52786624-52786643 Msa0169600:CDS 40.0%
CTGTAGTTATGACAATGAGG+AGG - chr1_4:52786738-52786757 Msa0169600:CDS 40.0%
TAGTTATGACAATGAGGAGG+AGG - chr1_4:52786741-52786760 Msa0169600:CDS 40.0%
GGAGGATGAAATAGTGTATG+AGG - chr1_4:52786759-52786778 Msa0169600:CDS 40.0%
CGGTAGAGAATCTTACTCAA+TGG - chr1_4:52786995-52787014 Msa0169600:intron 40.0%
!! TGCTAGTCTTTCAAACTGGT+TGG - chr1_4:52787194-52787213 Msa0169600:intron 40.0%
ATCGAATTGTGATGCTTCTG+TGG - chr1_4:52787308-52787327 Msa0169600:five_prime_UTR 40.0%
AGAAACTCGTTGGAGCCAAA+CGG - chr1_4:52786007-52786026 Msa0169600:three_prime_UTR 45.0%
ACTCTCTACAAAGTCCTAGC+TGG - chr1_4:52786243-52786262 Msa0169600:CDS 45.0%
! GGCAAACCCTAGTTCTTATC+AGG - chr1_4:52786603-52786622 Msa0169600:CDS 45.0%
ACTGTGGAATCAGAGAGAGA+AGG - chr1_4:52786667-52786686 Msa0169600:CDS 45.0%
!! ATTCGACGGGTATCGAATCA+TGG + chr1_4:52786704-52786723 None:intergenic 45.0%
GAGCTTGATTCCAAGACTAC+AGG + chr1_4:52787102-52787121 None:intergenic 45.0%
CAACAAAGGCTTCGACTGAA+TGG + chr1_4:52787157-52787176 None:intergenic 45.0%
ACTGCAACTTCCTGCAACAA+AGG + chr1_4:52787171-52787190 None:intergenic 45.0%
AGAAACTCGTTGGAGCCAAA+CGG - chr1_4:52786007-52786026 Msa0169600:three_prime_UTR 45.0%
ACTCTCTACAAAGTCCTAGC+TGG - chr1_4:52786243-52786262 Msa0169600:CDS 45.0%
! GGCAAACCCTAGTTCTTATC+AGG - chr1_4:52786603-52786622 Msa0169600:CDS 45.0%
ACTGTGGAATCAGAGAGAGA+AGG - chr1_4:52786667-52786686 Msa0169600:CDS 45.0%
!! ATTCGACGGGTATCGAATCA+TGG + chr1_4:52786704-52786723 None:intergenic 45.0%
GAGCTTGATTCCAAGACTAC+AGG + chr1_4:52787102-52787121 None:intergenic 45.0%
CAACAAAGGCTTCGACTGAA+TGG + chr1_4:52787157-52787176 None:intergenic 45.0%
ACTGCAACTTCCTGCAACAA+AGG + chr1_4:52787171-52787190 None:intergenic 45.0%
CGAATTTGAGTGGACGAGAG+AGG - chr1_4:52786944-52786963 Msa0169600:CDS 50.0%
CATGAATTCCGTTCTGCACC+CGG - chr1_4:52786975-52786994 Msa0169600:intron 50.0%
ATTCTCTACCGGGTGCAGAA+CGG + chr1_4:52786986-52787005 None:intergenic 50.0%
AAGCATGCTGCCTGTAGTCT+TGG - chr1_4:52787089-52787108 Msa0169600:CDS 50.0%
GCATTCGATTCCAAGACGCA+AGG + chr1_4:52787135-52787154 None:intergenic 50.0%
TCAGTCGAAGCCTTTGTTGC+AGG - chr1_4:52787158-52787177 Msa0169600:intron 50.0%
CGAATTTGAGTGGACGAGAG+AGG - chr1_4:52786944-52786963 Msa0169600:CDS 50.0%
CATGAATTCCGTTCTGCACC+CGG - chr1_4:52786975-52786994 Msa0169600:intron 50.0%
ATTCTCTACCGGGTGCAGAA+CGG + chr1_4:52786986-52787005 None:intergenic 50.0%
AAGCATGCTGCCTGTAGTCT+TGG - chr1_4:52787089-52787108 Msa0169600:CDS 50.0%
GCATTCGATTCCAAGACGCA+AGG + chr1_4:52787135-52787154 None:intergenic 50.0%
TCAGTCGAAGCCTTTGTTGC+AGG - chr1_4:52787158-52787177 Msa0169600:intron 50.0%
GAAGTTCTCGCCTTGCGTCT+TGG - chr1_4:52787122-52787141 Msa0169600:intron 55.0%
GAAGTTCTCGCCTTGCGTCT+TGG - chr1_4:52787122-52787141 Msa0169600:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 52785987 52787499 52785987 ID=Msa0169600;Name=Msa0169600
chr1_4 mRNA 52785987 52787499 52785987 ID=Msa0169600-mRNA-1;Parent=Msa0169600;Name=Msa0169600-mRNA-1;_AED=0.03;_eAED=0.03;_QI=202|1|1|1|1|1|3|239|299
chr1_4 exon 52785987 52786970 52785987 ID=Msa0169600-mRNA-1:exon:15698;Parent=Msa0169600-mRNA-1
chr1_4 exon 52787064 52787131 52787064 ID=Msa0169600-mRNA-1:exon:15697;Parent=Msa0169600-mRNA-1
chr1_4 exon 52787211 52787499 52787211 ID=Msa0169600-mRNA-1:exon:15696;Parent=Msa0169600-mRNA-1
chr1_4 five_prime_UTR 52787298 52787499 52787298 ID=Msa0169600-mRNA-1:five_prime_utr;Parent=Msa0169600-mRNA-1
chr1_4 CDS 52787211 52787297 52787211 ID=Msa0169600-mRNA-1:cds;Parent=Msa0169600-mRNA-1
chr1_4 CDS 52787064 52787131 52787064 ID=Msa0169600-mRNA-1:cds;Parent=Msa0169600-mRNA-1
chr1_4 CDS 52786226 52786970 52786226 ID=Msa0169600-mRNA-1:cds;Parent=Msa0169600-mRNA-1
chr1_4 three_prime_UTR 52785987 52786225 52785987 ID=Msa0169600-mRNA-1:three_prime_utr;Parent=Msa0169600-mRNA-1
Gene Sequence

>Msa0169600

ATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACAAGCAAAAGAAAACGCAGAAAGACTCTCTACAAAGTCCTAGCTGGAGAAAACCAAAAATATGGAAACTATCAAGTTCTTGCTACTATTACAGAGAAATCTATTGTACCATATTCTCAAATCAGAGGTAGAGATCAAGAAAAGGAGAAGAGTGGTGTTAAGAGCAAGAAGAAGAAGAAAGTTAGCTTTAATTTGAATGTAGAGATGTTTGAGGCAAACCCTAGTTCTTATCAGGTTTTGGATGATGATGATGAGGAAGATGAAAATAAGGAGACTACTGTGGAATCAGAGAGAGAAGGATCTGTAGTGTCCATGATTCGATACCCGTCGAATCATAGATATCATAACTGTAGTTATGACAATGAGGAGGAGGATGAAATAGTGTATGAGGAATCTGATATTGATGAGTTTGATGATGATGAATTTGATGAAGGGTATGATTGGGATGATGAAAGTTTGGAGAATTTTGGGGATGCTGAAGTTTGTGACGAAAACAGTGAACAGAAAGAAGATGGAGTGAAGAATCATAAGTCGAACGATGCTGAATTGAAATCGAATTTGAGTGGACGAGAGAGGAGCATAAACATGAATTCCGTTCTGCACCCGGTAGAGAATCTTACTCAATGGAAAGCAATCAAAGCAAAAGTTGCATCAAGCAAACAAACGAGAAAGGAAAATGTTCCATCAGAGCAAAAAACAAGCATGCTGCCTGTAGTCTTGGAATCAAGCTCGAAGTTCTCGCCTTGCGTCTTGGAATCGAATGCCATTCAGTCGAAGCCTTTGTTGCAGGAAGTTGCAGTTGATGCTAGTCTTTCAAACTGGTTGGTTTCTCCTAGTTATAATGTCTCCACTACAATTCGTTGTCAATGA
Protein sequence

>Msa0169600

MGCFLQCFGLTSKRKRRKTLYKVLAGENQKYGNYQVLATITEKSIVPYSQIRGRDQEKEKSGVKSKKKKKVSFNLNVEMFEANPSSYQVLDDDDEEDENKETTVESEREGSVVSMIRYPSNHRYHNCSYDNEEEDEIVYEESDIDEFDDDEFDEGYDWDDESLENFGDAEVCDENSEQKEDGVKNHKSNDAELKSNLSGRERSINMNSVLHPVENLTQWKAIKAKVASSKQTRKENVPSEQKTSMLPVVLESSSKFSPCVLESNAIQSKPLLQEVAVDASLSNWLVSPSYNVSTTIRCQ*