AlfalfaGEDB | Msa0189400

M. sativa cultivar zhongmu-4 / Msa0189400

KEGG: -
GO: GO:0000041 GO:0000302 GO:0003674 GO:0005215 GO:0005381 GO:0005384 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006828 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0006950 GO:0006979 GO:0008150 GO:0008324 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0032870 GO:0033554 GO:0034220 GO:0034599 GO:0034614 GO:0034755 GO:0035690 GO:0042221 GO:0042493 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051716 GO:0055065 GO:0055071 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071421 GO:0071495 GO:0071731 GO:0071732 GO:0097366 GO:0097577 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170
PFAM: PF01988.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0189400	XP_003593128.2	99.528	212	1	0	1	212	1	212	6.62e-151	427

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0189400	sp\|Q9M2C0\|VITH4_ARATH	68.718	195	57	2	14	207	2	193	1.11e-90	267

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0189400	G7IL91	99.528	212	1	0	1	212	1	212	3.16e-151	427

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0189400	Msa0231920	0.845049	5.174524e-59	-8.615850e-47
Msa0189400	Msa0271220	0.845312	4.393965e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0189400	MtrunA17_Chr2g0278621	99.528	212	1	0	1	212	1	212	6.09e-155	427
Msa0189400	MtrunA17_Chr6g0479471	99.057	212	2	0	1	212	1	212	5.01e-154	425
Msa0189400	MtrunA17_Chr1g0202031	70.652	184	49	1	29	207	29	212	4.85e-87	256
Msa0189400	MtrunA17_Chr6g0463641	66.667	195	55	2	23	207	11	205	8.49e-77	229
Msa0189400	MtrunA17_Chr4g0050831	60.938	192	66	1	27	209	30	221	1.37e-75	227
Msa0189400	MtrunA17_Chr4g0050821	60.938	192	66	1	27	209	30	221	1.40e-75	227
Msa0189400	MtrunA17_Chr4g0050841	61.579	190	64	1	27	207	30	219	1.78e-75	227
Msa0189400	MtrunA17_Chr4g0050811	56.279	215	83	3	1	209	27	236	9.47e-73	220
Msa0189400	MtrunA17_Chr4g0050851	50.463	216	95	1	7	210	13	228	1.46e-63	197

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0189400	AT3G43660.1	68.718	195	57	2	14	207	2	193	1.13e-91	267
Msa0189400	AT3G43630.1	71.277	188	50	2	21	207	8	192	3.22e-85	251
Msa0189400	AT1G21140.1	70.312	192	53	2	17	207	7	195	8.37e-84	247
Msa0189400	AT1G76800.1	68.984	187	54	1	21	207	9	191	1.53e-78	234
Msa0189400	AT3G25190.1	61.579	190	60	2	31	207	23	212	6.64e-64	197

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCTATGTCTCTTACTTT+TGG	0.225220	2_1:+4814650	Msa0189400:CDS
AGCCTTGCTTTGTTTGCTTT+TGG	0.248729	2_1:+4814557	Msa0189400:CDS
GAAGCAAAGGCAGCATAATA+AGG	0.258027	2_1:-4814437	None:intergenic
GGCTCCAACACCCATCATTA+AGG	0.342144	2_1:-4814246	None:intergenic
TGTTCTTGGGAAAGCTCCTT+TGG	0.348881	2_1:+4814592	Msa0189400:CDS
TTTGGTGAAGTCTTCTTTGA+GGG	0.356177	2_1:+4814610	Msa0189400:CDS
GTTAAGACAATGATTCTAAC+TGG	0.372301	2_1:+4814281	Msa0189400:CDS
GGATTGTTGAGTGCTGTTCT+TGG	0.377078	2_1:+4814578	Msa0189400:CDS
CTCAAAGAGCACAATGGCTT+AGG	0.396117	2_1:+4814180	Msa0189400:CDS
TTATTATGCTGCCTTTGCTT+CGG	0.407157	2_1:+4814439	Msa0189400:CDS
CTTTGGTGAAGTCTTCTTTG+AGG	0.408112	2_1:+4814609	Msa0189400:CDS
ATGATTCTAACTGGAATTGC+TGG	0.418092	2_1:+4814290	Msa0189400:CDS
GATTGTTGAGTGCTGTTCTT+GGG	0.419290	2_1:+4814579	Msa0189400:CDS
GGCGTTTGTTAAAGACTATA+AGG	0.421985	2_1:+4814505	Msa0189400:CDS
AACAAACGCCGCGCCGAGTA+GGG	0.424999	2_1:-4814492	None:intergenic
CTCAACAGCATCCTTAATGA+TGG	0.435656	2_1:+4814235	Msa0189400:CDS
TAAAGACTATAAGGTGAGGT+TGG	0.443344	2_1:+4814514	Msa0189400:CDS
GGAATTGCTGGTCTTGTAGC+TGG	0.462496	2_1:+4814302	Msa0189400:CDS
GTGAGGTTGGGAGTTGTGGT+TGG	0.470924	2_1:+4814527	Msa0189400:CDS
AAAAGCGATGGCCGAAGCAA+AGG	0.483514	2_1:-4814450	None:intergenic
TCAAAGAGCACAATGGCTTA+GGG	0.493819	2_1:+4814181	Msa0189400:CDS
TAAGGTGAGGTTGGGAGTTG+TGG	0.497331	2_1:+4814523	Msa0189400:CDS
AAAGACTATAAGGTGAGGTT+GGG	0.502612	2_1:+4814515	Msa0189400:CDS
TAACAAACGCCGCGCCGAGT+AGG	0.505245	2_1:-4814493	None:intergenic
AAGCAAAGGCAGCATAATAA+GGG	0.516802	2_1:-4814436	None:intergenic
GGCGCCTTTGTGCCCCTACT+CGG	0.521389	2_1:+4814479	Msa0189400:CDS
GGCGCCGACCGCAAAAGCGA+TGG	0.528738	2_1:-4814462	None:intergenic
GCATCCTTAATGATGGGTGT+TGG	0.549500	2_1:+4814242	Msa0189400:CDS
ATCCAAAAGCAAACAAAGCA+AGG	0.566512	2_1:-4814559	None:intergenic
AAGGCAGCATAATAAGGGTT+AGG	0.567356	2_1:-4814431	None:intergenic
GGTGCATGTAGCATGGCAAT+TGG	0.569674	2_1:+4814323	Msa0189400:CDS
CGCGCCGAGTAGGGGCACAA+AGG	0.576246	2_1:-4814483	None:intergenic
TCAACAGCATCCTTAATGAT+GGG	0.587391	2_1:+4814236	Msa0189400:CDS
ACAAACGCCGCGCCGAGTAG+GGG	0.622593	2_1:-4814491	None:intergenic
CTCAAAGAAGACTTCACCAA+AGG	0.626739	2_1:-4814608	None:intergenic
TGTAGCTGGTGCATGTAGCA+TGG	0.629799	2_1:+4814316	Msa0189400:CDS
TTGTTAAAGACTATAAGGTG+AGG	0.635278	2_1:+4814510	Msa0189400:CDS
TACGTGCTAGTGTAGCTGCA+TGG	0.662565	2_1:-4814088	None:intergenic
ATTATACTCAAAGAGCACAA+TGG	0.665811	2_1:+4814174	Msa0189400:CDS
CTTTGTGCCCCTACTCGGCG+CGG	0.685778	2_1:+4814484	Msa0189400:CDS
TGAATCAAAAGTGTACAACA+TGG	0.690093	2_1:+4814112	Msa0189400:CDS
TTTGCACAAATGAAAAGACA+AGG	0.695742	2_1:+4814383	Msa0189400:CDS
AAAATGATCAAACTACGACA+TGG	0.707756	2_1:-4814691	None:intergenic
GTGCTAGTGTAGCTGCATGG+TGG	0.751494	2_1:-4814085	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATGGATGTTGAAAAAAA+AGG	+	chr2_1:4814128-4814147	Msa0189400:CDS	25.0%
!!	TGTCTTAACATCTTTTGTAA+CGG	-	chr2_1:4814270-4814289	None:intergenic	25.0%
!	TGAATCAAAAGTGTACAACA+TGG	+	chr2_1:4814112-4814131	Msa0189400:CDS	30.0%
!	ATTATACTCAAAGAGCACAA+TGG	+	chr2_1:4814174-4814193	Msa0189400:CDS	30.0%
	GTTAAGACAATGATTCTAAC+TGG	+	chr2_1:4814281-4814300	Msa0189400:CDS	30.0%
	TTTGCACAAATGAAAAGACA+AGG	+	chr2_1:4814383-4814402	Msa0189400:CDS	30.0%
	TTGTTAAAGACTATAAGGTG+AGG	+	chr2_1:4814510-4814529	Msa0189400:CDS	30.0%
	TCAACAGCATCCTTAATGAT+GGG	+	chr2_1:4814236-4814255	Msa0189400:CDS	35.0%
	ATGATTCTAACTGGAATTGC+TGG	+	chr2_1:4814290-4814309	Msa0189400:CDS	35.0%
	AAGCAAAGGCAGCATAATAA+GGG	-	chr2_1:4814439-4814458	None:intergenic	35.0%
	TTATTATGCTGCCTTTGCTT+CGG	+	chr2_1:4814439-4814458	Msa0189400:CDS	35.0%
!	GGCGTTTGTTAAAGACTATA+AGG	+	chr2_1:4814505-4814524	Msa0189400:CDS	35.0%
	TAAAGACTATAAGGTGAGGT+TGG	+	chr2_1:4814514-4814533	Msa0189400:CDS	35.0%
	AAAGACTATAAGGTGAGGTT+GGG	+	chr2_1:4814515-4814534	Msa0189400:CDS	35.0%
	ATCCAAAAGCAAACAAAGCA+AGG	-	chr2_1:4814562-4814581	None:intergenic	35.0%
	TTTGGTGAAGTCTTCTTTGA+GGG	+	chr2_1:4814610-4814629	Msa0189400:CDS	35.0%
!!	TCTTCTTTGAGGGTTTTGAT+TGG	+	chr2_1:4814620-4814639	Msa0189400:CDS	35.0%
!!	CTTGCTATGTCTCTTACTTT+TGG	+	chr2_1:4814650-4814669	Msa0189400:CDS	35.0%
!	TCAAAGAGCACAATGGCTTA+GGG	+	chr2_1:4814181-4814200	Msa0189400:CDS	40.0%
!!	GCTGTTTTAGGTGCTAATGA+TGG	+	chr2_1:4814206-4814225	Msa0189400:CDS	40.0%
	CTCAACAGCATCCTTAATGA+TGG	+	chr2_1:4814235-4814254	Msa0189400:CDS	40.0%
	AAGGCAGCATAATAAGGGTT+AGG	-	chr2_1:4814434-4814453	None:intergenic	40.0%
	GAAGCAAAGGCAGCATAATA+AGG	-	chr2_1:4814440-4814459	None:intergenic	40.0%
!!!	AGCCTTGCTTTGTTTGCTTT+TGG	+	chr2_1:4814557-4814576	Msa0189400:CDS	40.0%
!	GATTGTTGAGTGCTGTTCTT+GGG	+	chr2_1:4814579-4814598	Msa0189400:CDS	40.0%
	CTCAAAGAAGACTTCACCAA+AGG	-	chr2_1:4814611-4814630	None:intergenic	40.0%
	CTTTGGTGAAGTCTTCTTTG+AGG	+	chr2_1:4814609-4814628	Msa0189400:CDS	40.0%
!!!	TCTTTGAGGGTTTTGATTGG+TGG	+	chr2_1:4814623-4814642	Msa0189400:CDS	40.0%
!	CTCAAAGAGCACAATGGCTT+AGG	+	chr2_1:4814180-4814199	Msa0189400:CDS	45.0%
!!	GCATCCTTAATGATGGGTGT+TGG	+	chr2_1:4814242-4814261	Msa0189400:CDS	45.0%
!	GGATTGTTGAGTGCTGTTCT+TGG	+	chr2_1:4814578-4814597	Msa0189400:CDS	45.0%
	TGTTCTTGGGAAAGCTCCTT+TGG	+	chr2_1:4814592-4814611	Msa0189400:CDS	45.0%
!!!	TGAGGGTTTTGATTGGTGGA+TGG	+	chr2_1:4814627-4814646	Msa0189400:CDS	45.0%
	TACGTGCTAGTGTAGCTGCA+TGG	-	chr2_1:4814091-4814110	None:intergenic	50.0%
!!	TGGCTTAGGGCAGCTGTTTT+AGG	+	chr2_1:4814194-4814213	Msa0189400:CDS	50.0%
	GGCTCCAACACCCATCATTA+AGG	-	chr2_1:4814249-4814268	None:intergenic	50.0%
!!	GGAATTGCTGGTCTTGTAGC+TGG	+	chr2_1:4814302-4814321	Msa0189400:CDS	50.0%
	TGTAGCTGGTGCATGTAGCA+TGG	+	chr2_1:4814316-4814335	Msa0189400:CDS	50.0%
	GGTGCATGTAGCATGGCAAT+TGG	+	chr2_1:4814323-4814342	Msa0189400:CDS	50.0%
	AAAAGCGATGGCCGAAGCAA+AGG	-	chr2_1:4814453-4814472	None:intergenic	50.0%
	TAAGGTGAGGTTGGGAGTTG+TGG	+	chr2_1:4814523-4814542	Msa0189400:CDS	50.0%
	GTGCTAGTGTAGCTGCATGG+TGG	-	chr2_1:4814088-4814107	None:intergenic	55.0%
!!	TGCTTCGGCCATCGCTTTTG+CGG	+	chr2_1:4814454-4814473	Msa0189400:CDS	55.0%
	GTGAGGTTGGGAGTTGTGGT+TGG	+	chr2_1:4814527-4814546	Msa0189400:CDS	55.0%
!!	TCGGCCATCGCTTTTGCGGT+CGG	+	chr2_1:4814458-4814477	Msa0189400:CDS	60.0%
	AACAAACGCCGCGCCGAGTA+GGG	-	chr2_1:4814495-4814514	None:intergenic	60.0%
	TAACAAACGCCGCGCCGAGT+AGG	-	chr2_1:4814496-4814515	None:intergenic	60.0%
	GGCGCCTTTGTGCCCCTACT+CGG	+	chr2_1:4814479-4814498	Msa0189400:CDS	65.0%
	CTTTGTGCCCCTACTCGGCG+CGG	+	chr2_1:4814484-4814503	Msa0189400:CDS	65.0%
	ACAAACGCCGCGCCGAGTAG+GGG	-	chr2_1:4814494-4814513	None:intergenic	65.0%
!!	GGCGCCGACCGCAAAAGCGA+TGG	-	chr2_1:4814465-4814484	None:intergenic	70.0%
!	CGCGCCGAGTAGGGGCACAA+AGG	-	chr2_1:4814486-4814505	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	4814068	4814706	4814068	ID=Msa0189400;Name=Msa0189400
chr2_1	mRNA	4814068	4814706	4814068	ID=Msa0189400-mRNA-1;Parent=Msa0189400;Name=Msa0189400-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|212
chr2_1	exon	4814068	4814706	4814068	ID=Msa0189400-mRNA-1:exon:2130;Parent=Msa0189400-mRNA-1
chr2_1	CDS	4814068	4814706	4814068	ID=Msa0189400-mRNA-1:cds;Parent=Msa0189400-mRNA-1

Gene Sequence

>Msa0189400

ATGGCAGAAATTCAGCACCACCATGCAGCTACACTAGCACGTAGTGAATCAAAAGTGTACAACATGGATGTTGAAAAAAAAGGAGAAGAAGAAAATGATGAAAAAGATTATACTCAAAGAGCACAATGGCTTAGGGCAGCTGTTTTAGGTGCTAATGATGGTTTACTCTCAACAGCATCCTTAATGATGGGTGTTGGAGCCGTTACAAAAGATGTTAAGACAATGATTCTAACTGGAATTGCTGGTCTTGTAGCTGGTGCATGTAGCATGGCAATTGGTGAATTTGTGTCTGTTTATTCACAATATGATATTGAGTTTGCACAAATGAAAAGACAAGGAAACATTTCTCAAAAAGATAAATTGCCTAACCCTTATTATGCTGCCTTTGCTTCGGCCATCGCTTTTGCGGTCGGCGCCTTTGTGCCCCTACTCGGCGCGGCGTTTGTTAAAGACTATAAGGTGAGGTTGGGAGTTGTGGTTGGTGTTGTTAGCCTTGCTTTGTTTGCTTTTGGATTGTTGAGTGCTGTTCTTGGGAAAGCTCCTTTGGTGAAGTCTTCTTTGAGGGTTTTGATTGGTGGATGGCTTGCTATGTCTCTTACTTTTGGTTTAACTAAGCTTGTAAACCATGTCGTAGTTTGA

Protein sequence

>Msa0189400

MAEIQHHHAATLARSESKVYNMDVEKKGEEENDEKDYTQRAQWLRAAVLGANDGLLSTASLMMGVGAVTKDVKTMILTGIAGLVAGACSMAIGEFVSVYSQYDIEFAQMKRQGNISQKDKLPNPYYAAFASAIAFAVGAFVPLLGAAFVKDYKVRLGVVVGVVSLALFAFGLLSAVLGKAPLVKSSLRVLIGGWLAMSLTFGLTKLVNHVVV*