AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0195160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195160 MtrunA17_Chr2g0292421 99.281 139 1 0 1 139 1 139 1.58e-99 290
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195160 AT4G05390.1 64.925 134 44 3 7 139 8 139 6.15e-56 179
Msa0195160 AT1G30510.1 64.085 142 48 2 1 139 1 142 2.56e-52 170
Msa0195160 AT1G30510.2 64.336 143 47 3 1 139 1 143 1.39e-51 168
Msa0195160 AT1G30510.3 80.769 78 15 0 62 139 1 78 1.84e-41 140
Msa0195160 AT4G05390.2 52.985 134 32 3 7 139 8 111 4.71e-39 135
Msa0195160 AT1G20020.3 40.541 74 44 0 69 142 66 139 1.82e-11 60.8
Msa0195160 AT1G20020.1 40.541 74 44 0 69 142 66 139 1.91e-11 60.8
Msa0195160 AT5G66190.1 51.786 56 27 0 87 142 75 130 2.57e-11 60.5

Find 61 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCAGAAGATCTGTGTTTA+AGG 0.082604 2_1:-11928951 Msa0195160:intron
ATGAATTTACATTCAGTATT+TGG 0.154698 2_1:+11927849 None:intergenic
GGCATCTAACATAAACTTTC+GGG 0.262545 2_1:-11928202 Msa0195160:CDS
GGAAACAATTGTTGCTGTAT+AGG 0.274117 2_1:+11927999 None:intergenic
TGTTCTTCCCTTTGGCTTCT+CGG 0.276066 2_1:+11929471 None:intergenic
TGCTGTCTCCCCTTTAGAAT+TGG 0.295412 2_1:-11928067 Msa0195160:CDS
GTTGCTGTATAGGGTTCTTT+AGG 0.297996 2_1:+11928009 None:intergenic
TTGGATAACAAGTCACTTAC+TGG 0.298491 2_1:+11927868 None:intergenic
CGGGTTCTTGTTCTTCCCTT+TGG 0.314253 2_1:+11929463 None:intergenic
GAAACAATTGTTGCTGTATA+GGG 0.331471 2_1:+11928000 None:intergenic
CAAGTTTCCCCAGGAGCCTT+TGG 0.352435 2_1:+11927958 None:intergenic
GTCTCACCTGTCAGCTTCTC+AGG 0.374975 2_1:-11929297 Msa0195160:intron
TGTTTCCGTTGAACGAATAG+TGG 0.377041 2_1:-11927983 Msa0195160:CDS
AGGCATCTAACATAAACTTT+CGG 0.378017 2_1:-11928203 Msa0195160:CDS
AACCTTAAACACAGATCTTC+TGG 0.380352 2_1:+11928949 None:intergenic
ATGGGCACCGGTGTTTGCTT+TGG 0.380687 2_1:-11928172 Msa0195160:CDS
TAAAGGGGAGACAGCAACTT+TGG 0.385969 2_1:+11928074 None:intergenic
TTAGGCTTGTGTAGGTTCAA+TGG 0.400594 2_1:+11928027 None:intergenic
TAAACTTTCGGGATAAATCA+TGG 0.423327 2_1:-11928191 Msa0195160:CDS
AAAGGGGAGACAGCAACTTT+GGG 0.448601 2_1:+11928075 None:intergenic
CATGATTGATAATGAGGAAG+AGG 0.450727 2_1:+11929319 None:intergenic
GCGGAGAAATCATTGCTAAC+AGG 0.451084 2_1:+11928971 None:intergenic
ATGATGGAAATGTTCCCTAC+TGG 0.456298 2_1:-11927918 Msa0195160:CDS
ATAGTGGGACCAAAGGCTCC+TGG 0.469565 2_1:-11927967 Msa0195160:CDS
AGGTTCAATGGAGGCTCAGC+AGG 0.479247 2_1:+11928039 None:intergenic
AGGGTTCTTTAGGCTTGTGT+AGG 0.483001 2_1:+11928019 None:intergenic
GTGAGACATGATTGATAATG+AGG 0.503169 2_1:+11929313 None:intergenic
TACTGGGAGGGACAGAGTTA+TGG 0.510183 2_1:-11927901 Msa0195160:CDS
ATGAATGATCCTTTCGAGAG+AGG 0.521646 2_1:+11929433 None:intergenic
TAGTGGGACCAAAGGCTCCT+GGG 0.524756 2_1:-11927966 Msa0195160:CDS
TGAATGATCCTTTCGAGAGA+GGG 0.526047 2_1:+11929434 None:intergenic
TGATGGAAATGTTCCCTACT+GGG 0.542317 2_1:-11927917 Msa0195160:CDS
TGGTCCCACTATTCGTTCAA+CGG 0.545920 2_1:+11927978 None:intergenic
TGGAGTTAAGAATTCCAAGT+TGG 0.547006 2_1:-11929507 None:intergenic
TGAAAGCAAAGAACTGTGGT+TGG 0.552421 2_1:-11928146 Msa0195160:CDS
GATCCTTTCGAGAGAGGGAA+AGG 0.554605 2_1:+11929439 None:intergenic
CTTTCGAGAGAGGGAAAGGT+CGG 0.556230 2_1:+11929443 None:intergenic
CGACCTTTCCCTCTCTCGAA+AGG 0.556712 2_1:-11929442 Msa0195160:five_prime_UTR
GGCTTGTGTAGGTTCAATGG+AGG 0.564256 2_1:+11928030 None:intergenic
AAACTTTCGGGATAAATCAT+GGG 0.573214 2_1:-11928190 Msa0195160:CDS
TTTCGAGAGAGGGAAAGGTC+GGG 0.578165 2_1:+11929444 None:intergenic
AAGCAAAGAACTGTGGTTGG+AGG 0.580752 2_1:-11928143 Msa0195160:CDS
ATAACTCTGTCCCTCCCAGT+AGG 0.582469 2_1:+11927903 None:intergenic
CTACACTCCGAGAAGCCAAA+GGG 0.584508 2_1:-11929478 Msa0195160:five_prime_UTR
TAACTCTGTCCCTCCCAGTA+GGG 0.586338 2_1:+11927904 None:intergenic
GTTTCCGTTGAACGAATAGT+GGG 0.586815 2_1:-11927982 Msa0195160:CDS
TGATTACCTGAGAAGCTGAC+AGG 0.587040 2_1:+11929291 None:intergenic
CATGTTGTGATCAATCATGA+TGG 0.590540 2_1:-11927934 Msa0195160:CDS
GAGTGTAGTCTTCGCCAACT+TGG 0.591601 2_1:+11929493 None:intergenic
TCGGGATAAATCATGGGCAC+CGG 0.594074 2_1:-11928184 Msa0195160:CDS
GATAACAAGTCACTTACTGG+TGG 0.605866 2_1:+11927871 None:intergenic
ACTACACTCCGAGAAGCCAA+AGG 0.627391 2_1:-11929479 Msa0195160:five_prime_UTR
GGAAATGTTCCCTACTGGGA+GGG 0.629303 2_1:-11927913 Msa0195160:CDS
AGTGGGACCAAAGGCTCCTG+GGG 0.631009 2_1:-11927965 Msa0195160:CDS
ACAACATGACAAGTTTCCCC+AGG 0.635964 2_1:+11927949 None:intergenic
TTCATATCCAAAGCAAACAC+CGG 0.638479 2_1:+11928165 None:intergenic
TGAACGAATAGTGGGACCAA+AGG 0.654010 2_1:-11927974 Msa0195160:CDS
TGGAAATGTTCCCTACTGGG+AGG 0.698603 2_1:-11927914 Msa0195160:CDS
GATATGAAAGCAAAGAACTG+TGG 0.703274 2_1:-11928150 Msa0195160:CDS
ATAGTAGTAGAGTAGTAACG+AGG 0.707605 2_1:+11929365 None:intergenic
CTTAAACACAGATCTTCTGG+CGG 0.716512 2_1:+11928952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTCTTTTCAATAT+GGG - chr2_1:11928170-11928189 Msa0195160:CDS 10.0%
!!! ATAAATAAAAAGTACAAATT+GGG + chr2_1:11928264-11928283 None:intergenic 10.0%
!!! AATAAATAAAAAGTACAAAT+TGG + chr2_1:11928265-11928284 None:intergenic 10.0%
!!! TTTTTATTTCTTTTCAATAT+GGG - chr2_1:11928170-11928189 Msa0195160:CDS 10.0%
!!! ATAAATAAAAAGTACAAATT+GGG + chr2_1:11928264-11928283 None:intergenic 10.0%
!!! AATAAATAAAAAGTACAAAT+TGG + chr2_1:11928265-11928284 None:intergenic 10.0%
!! CATATTGAAAAGAAATAAAA+AGG + chr2_1:11928171-11928190 None:intergenic 15.0%
!!! CTTTTTATTTCTTTTCAATA+TGG - chr2_1:11928169-11928188 Msa0195160:CDS 15.0%
!! TGAATGAATATATTTATTTG+CGG - chr2_1:11928208-11928227 Msa0195160:intron 15.0%
!!! TAAATAAAAAGTACAAATTG+GGG + chr2_1:11928263-11928282 None:intergenic 15.0%
!! TTAAAAAATATGAAGTAAAG+TGG + chr2_1:11928508-11928527 None:intergenic 15.0%
!!! TTTTTATGACTAATTTGATT+TGG - chr2_1:11928626-11928645 Msa0195160:intron 15.0%
!!! ATTTGATTTGGTAAATTTTA+TGG - chr2_1:11928638-11928657 Msa0195160:intron 15.0%
!! CAAATGAATAATATTATGTT+AGG - chr2_1:11928849-11928868 Msa0195160:intron 15.0%
!! CATATTGAAAAGAAATAAAA+AGG + chr2_1:11928171-11928190 None:intergenic 15.0%
!!! CTTTTTATTTCTTTTCAATA+TGG - chr2_1:11928169-11928188 Msa0195160:CDS 15.0%
!! TGAATGAATATATTTATTTG+CGG - chr2_1:11928208-11928227 Msa0195160:intron 15.0%
!!! TAAATAAAAAGTACAAATTG+GGG + chr2_1:11928263-11928282 None:intergenic 15.0%
!! TTAAAAAATATGAAGTAAAG+TGG + chr2_1:11928508-11928527 None:intergenic 15.0%
!!! TTTTTATGACTAATTTGATT+TGG - chr2_1:11928626-11928645 Msa0195160:intron 15.0%
!!! ATTTGATTTGGTAAATTTTA+TGG - chr2_1:11928638-11928657 Msa0195160:intron 15.0%
!! CAAATGAATAATATTATGTT+AGG - chr2_1:11928849-11928868 Msa0195160:intron 15.0%
!!! TAAAAAGGTGAAATTTTGAT+GGG + chr2_1:11928156-11928175 None:intergenic 20.0%
!!! ATAAAAAGGTGAAATTTTGA+TGG + chr2_1:11928157-11928176 None:intergenic 20.0%
!!! TTTTCCTTCACTTTTAAATA+TGG - chr2_1:11928441-11928460 Msa0195160:intron 20.0%
!!! TAATAAGCAATTATTTCTCT+TGG + chr2_1:11928799-11928818 None:intergenic 20.0%
!! GAAATAATTGCTTATTATTG+TGG - chr2_1:11928802-11928821 Msa0195160:intron 20.0%
!!! TACTTTGTTATGAGTTATTT+TGG - chr2_1:11928879-11928898 Msa0195160:intron 20.0%
!!! AATACAATCTCTTATTTTAG+TGG + chr2_1:11929056-11929075 None:intergenic 20.0%
!! TGTAATTTCTATTCTTGTTA+AGG - chr2_1:11929091-11929110 Msa0195160:intron 20.0%
!!! TAAAAAGGTGAAATTTTGAT+GGG + chr2_1:11928156-11928175 None:intergenic 20.0%
!!! ATAAAAAGGTGAAATTTTGA+TGG + chr2_1:11928157-11928176 None:intergenic 20.0%
!!! TTTTCCTTCACTTTTAAATA+TGG - chr2_1:11928441-11928460 Msa0195160:intron 20.0%
!!! TAATAAGCAATTATTTCTCT+TGG + chr2_1:11928799-11928818 None:intergenic 20.0%
!! GAAATAATTGCTTATTATTG+TGG - chr2_1:11928802-11928821 Msa0195160:intron 20.0%
!!! TACTTTGTTATGAGTTATTT+TGG - chr2_1:11928879-11928898 Msa0195160:intron 20.0%
!!! AATACAATCTCTTATTTTAG+TGG + chr2_1:11929056-11929075 None:intergenic 20.0%
!! TGTAATTTCTATTCTTGTTA+AGG - chr2_1:11929091-11929110 Msa0195160:intron 20.0%
!! TTCTTTTCAATATGGGTTTT+TGG - chr2_1:11928177-11928196 Msa0195160:CDS 25.0%
!! AATCAATACTCAAACTTTTG+TGG - chr2_1:11928330-11928349 Msa0195160:intron 25.0%
!! ATCAATACTCAAACTTTTGT+GGG - chr2_1:11928331-11928350 Msa0195160:intron 25.0%
!!! TTTTGTTTTGTTTTTGCAGA+TGG - chr2_1:11928356-11928375 Msa0195160:intron 25.0%
! TTACAATAATAGTTCCTTCA+AGG + chr2_1:11928675-11928694 None:intergenic 25.0%
! GGAACTATTATTGTAATTGA+TGG - chr2_1:11928679-11928698 Msa0195160:intron 25.0%
! ATACAGAAATGAACTGAATA+AGG + chr2_1:11928716-11928735 None:intergenic 25.0%
!! TTCTTTTCAATATGGGTTTT+TGG - chr2_1:11928177-11928196 Msa0195160:CDS 25.0%
!! AATCAATACTCAAACTTTTG+TGG - chr2_1:11928330-11928349 Msa0195160:intron 25.0%
!! ATCAATACTCAAACTTTTGT+GGG - chr2_1:11928331-11928350 Msa0195160:intron 25.0%
!!! TTTTGTTTTGTTTTTGCAGA+TGG - chr2_1:11928356-11928375 Msa0195160:intron 25.0%
! TTACAATAATAGTTCCTTCA+AGG + chr2_1:11928675-11928694 None:intergenic 25.0%
! GGAACTATTATTGTAATTGA+TGG - chr2_1:11928679-11928698 Msa0195160:intron 25.0%
! ATACAGAAATGAACTGAATA+AGG + chr2_1:11928716-11928735 None:intergenic 25.0%
! ATAAAAAGTACAAATTGGGG+TGG + chr2_1:11928260-11928279 None:intergenic 30.0%
!! TCCTTCACTTTTAAATATGG+CGG - chr2_1:11928444-11928463 Msa0195160:intron 30.0%
ACAGATCCTTTATACATTTG+GGG + chr2_1:11928596-11928615 None:intergenic 30.0%
CACAGATCCTTTATACATTT+GGG + chr2_1:11928597-11928616 None:intergenic 30.0%
TCACAGATCCTTTATACATT+TGG + chr2_1:11928598-11928617 None:intergenic 30.0%
TCATTTGCATCAAATCAAAG+TGG + chr2_1:11928836-11928855 None:intergenic 30.0%
!!! CGTGTCTAGTTTTGTTATAT+AGG - chr2_1:11928968-11928987 Msa0195160:CDS 30.0%
AGGCATCTAACATAAACTTT+CGG - chr2_1:11929158-11929177 Msa0195160:intron 30.0%
TAAACTTTCGGGATAAATCA+TGG - chr2_1:11929170-11929189 Msa0195160:intron 30.0%
AAACTTTCGGGATAAATCAT+GGG - chr2_1:11929171-11929190 Msa0195160:intron 30.0%
!! AGGATTTTCCAATTCTAAAG+GGG + chr2_1:11929305-11929324 None:intergenic 30.0%
! CAGGATTTTCCAATTCTAAA+GGG + chr2_1:11929306-11929325 None:intergenic 30.0%
GAAACAATTGTTGCTGTATA+GGG + chr2_1:11929364-11929383 None:intergenic 30.0%
! ATAAAAAGTACAAATTGGGG+TGG + chr2_1:11928260-11928279 None:intergenic 30.0%
!! TCCTTCACTTTTAAATATGG+CGG - chr2_1:11928444-11928463 Msa0195160:intron 30.0%
ACAGATCCTTTATACATTTG+GGG + chr2_1:11928596-11928615 None:intergenic 30.0%
CACAGATCCTTTATACATTT+GGG + chr2_1:11928597-11928616 None:intergenic 30.0%
TCACAGATCCTTTATACATT+TGG + chr2_1:11928598-11928617 None:intergenic 30.0%
TCATTTGCATCAAATCAAAG+TGG + chr2_1:11928836-11928855 None:intergenic 30.0%
!!! CGTGTCTAGTTTTGTTATAT+AGG - chr2_1:11928968-11928987 Msa0195160:CDS 30.0%
AGGCATCTAACATAAACTTT+CGG - chr2_1:11929158-11929177 Msa0195160:intron 30.0%
TAAACTTTCGGGATAAATCA+TGG - chr2_1:11929170-11929189 Msa0195160:intron 30.0%
AAACTTTCGGGATAAATCAT+GGG - chr2_1:11929171-11929190 Msa0195160:intron 30.0%
!! AGGATTTTCCAATTCTAAAG+GGG + chr2_1:11929305-11929324 None:intergenic 30.0%
! CAGGATTTTCCAATTCTAAA+GGG + chr2_1:11929306-11929325 None:intergenic 30.0%
GAAACAATTGTTGCTGTATA+GGG + chr2_1:11929364-11929383 None:intergenic 30.0%
ATAGTAGTAGAGTAGTAACG+AGG + chr2_1:11927999-11928018 None:intergenic 35.0%
!! CATGATTGATAATGAGGAAG+AGG + chr2_1:11928045-11928064 None:intergenic 35.0%
!! GTGAGACATGATTGATAATG+AGG + chr2_1:11928051-11928070 None:intergenic 35.0%
AGAACGCAAAAAGGAAGAAA+AGG + chr2_1:11928109-11928128 None:intergenic 35.0%
TTTGCAGATAGAACGCAAAA+AGG + chr2_1:11928118-11928137 None:intergenic 35.0%
! TTTTGCGTTCTATCTGCAAA+TGG - chr2_1:11928118-11928137 Msa0195160:CDS 35.0%
TTTGCGTTCTATCTGCAAAT+GGG - chr2_1:11928119-11928138 Msa0195160:CDS 35.0%
TTATTTGCGGAACTTCAAAG+TGG - chr2_1:11928221-11928240 Msa0195160:intron 35.0%
AACCTTAAACACAGATCTTC+TGG + chr2_1:11928415-11928434 None:intergenic 35.0%
!! CCTTCACTTTTAAATATGGC+GGG - chr2_1:11928445-11928464 Msa0195160:intron 35.0%
TGAAGTAAAGTGGAGTTTCA+TGG + chr2_1:11928498-11928517 None:intergenic 35.0%
CATAGACCCCAAATGTATAA+AGG - chr2_1:11928587-11928606 Msa0195160:intron 35.0%
! GACGGGTTTAAGTATTAGTA+TGG - chr2_1:11928748-11928767 Msa0195160:intron 35.0%
AAAACTGAACTTGATGTTGC+AGG - chr2_1:11929138-11929157 Msa0195160:intron 35.0%
GGCATCTAACATAAACTTTC+GGG - chr2_1:11929159-11929178 Msa0195160:intron 35.0%
TTCATATCCAAAGCAAACAC+CGG + chr2_1:11929199-11929218 None:intergenic 35.0%
GATATGAAAGCAAAGAACTG+TGG - chr2_1:11929211-11929230 Msa0195160:intron 35.0%
! GCAGGATTTTCCAATTCTAA+AGG + chr2_1:11929307-11929326 None:intergenic 35.0%
GGAAACAATTGTTGCTGTAT+AGG + chr2_1:11929365-11929384 None:intergenic 35.0%
CATGTTGTGATCAATCATGA+TGG - chr2_1:11929427-11929446 Msa0195160:five_prime_UTR 35.0%
TTGGATAACAAGTCACTTAC+TGG + chr2_1:11929496-11929515 None:intergenic 35.0%
ATAGTAGTAGAGTAGTAACG+AGG + chr2_1:11927999-11928018 None:intergenic 35.0%
!! CATGATTGATAATGAGGAAG+AGG + chr2_1:11928045-11928064 None:intergenic 35.0%
!! GTGAGACATGATTGATAATG+AGG + chr2_1:11928051-11928070 None:intergenic 35.0%
AGAACGCAAAAAGGAAGAAA+AGG + chr2_1:11928109-11928128 None:intergenic 35.0%
TTTGCAGATAGAACGCAAAA+AGG + chr2_1:11928118-11928137 None:intergenic 35.0%
! TTTTGCGTTCTATCTGCAAA+TGG - chr2_1:11928118-11928137 Msa0195160:CDS 35.0%
TTTGCGTTCTATCTGCAAAT+GGG - chr2_1:11928119-11928138 Msa0195160:CDS 35.0%
TTATTTGCGGAACTTCAAAG+TGG - chr2_1:11928221-11928240 Msa0195160:intron 35.0%
AACCTTAAACACAGATCTTC+TGG + chr2_1:11928415-11928434 None:intergenic 35.0%
!! CCTTCACTTTTAAATATGGC+GGG - chr2_1:11928445-11928464 Msa0195160:intron 35.0%
TGAAGTAAAGTGGAGTTTCA+TGG + chr2_1:11928498-11928517 None:intergenic 35.0%
CATAGACCCCAAATGTATAA+AGG - chr2_1:11928587-11928606 Msa0195160:intron 35.0%
! GACGGGTTTAAGTATTAGTA+TGG - chr2_1:11928748-11928767 Msa0195160:intron 35.0%
AAAACTGAACTTGATGTTGC+AGG - chr2_1:11929138-11929157 Msa0195160:intron 35.0%
GGCATCTAACATAAACTTTC+GGG - chr2_1:11929159-11929178 Msa0195160:intron 35.0%
TTCATATCCAAAGCAAACAC+CGG + chr2_1:11929199-11929218 None:intergenic 35.0%
GATATGAAAGCAAAGAACTG+TGG - chr2_1:11929211-11929230 Msa0195160:intron 35.0%
! GCAGGATTTTCCAATTCTAA+AGG + chr2_1:11929307-11929326 None:intergenic 35.0%
GGAAACAATTGTTGCTGTAT+AGG + chr2_1:11929365-11929384 None:intergenic 35.0%
CATGTTGTGATCAATCATGA+TGG - chr2_1:11929427-11929446 Msa0195160:five_prime_UTR 35.0%
TTGGATAACAAGTCACTTAC+TGG + chr2_1:11929496-11929515 None:intergenic 35.0%
TGAATGATCCTTTCGAGAGA+GGG + chr2_1:11927930-11927949 None:intergenic 40.0%
ATGAATGATCCTTTCGAGAG+AGG + chr2_1:11927931-11927950 None:intergenic 40.0%
CTTAAACACAGATCTTCTGG+CGG + chr2_1:11928412-11928431 None:intergenic 40.0%
CCCGCCATATTTAAAAGTGA+AGG + chr2_1:11928448-11928467 None:intergenic 40.0%
!! TGGCTTTTGAGAAACCTTGA+AGG - chr2_1:11928658-11928677 Msa0195160:intron 40.0%
!!! TTTTGGTTTTGCAAGACCTG+TGG - chr2_1:11928896-11928915 Msa0195160:intron 40.0%
! ACACAGACTTTGATAACCAC+AGG + chr2_1:11928915-11928934 None:intergenic 40.0%
TGAAAGCAAAGAACTGTGGT+TGG - chr2_1:11929215-11929234 Msa0195160:intron 40.0%
TTAGGCTTGTGTAGGTTCAA+TGG + chr2_1:11929337-11929356 None:intergenic 40.0%
GTTGCTGTATAGGGTTCTTT+AGG + chr2_1:11929355-11929374 None:intergenic 40.0%
TGTTTCCGTTGAACGAATAG+TGG - chr2_1:11929378-11929397 Msa0195160:five_prime_UTR 40.0%
GTTTCCGTTGAACGAATAGT+GGG - chr2_1:11929379-11929398 Msa0195160:five_prime_UTR 40.0%
! ATGATGGAAATGTTCCCTAC+TGG - chr2_1:11929443-11929462 Msa0195160:five_prime_UTR 40.0%
TGATGGAAATGTTCCCTACT+GGG - chr2_1:11929444-11929463 Msa0195160:five_prime_UTR 40.0%
GATAACAAGTCACTTACTGG+TGG + chr2_1:11929493-11929512 None:intergenic 40.0%
TGAATGATCCTTTCGAGAGA+GGG + chr2_1:11927930-11927949 None:intergenic 40.0%
ATGAATGATCCTTTCGAGAG+AGG + chr2_1:11927931-11927950 None:intergenic 40.0%
CTTAAACACAGATCTTCTGG+CGG + chr2_1:11928412-11928431 None:intergenic 40.0%
CCCGCCATATTTAAAAGTGA+AGG + chr2_1:11928448-11928467 None:intergenic 40.0%
!! TGGCTTTTGAGAAACCTTGA+AGG - chr2_1:11928658-11928677 Msa0195160:intron 40.0%
!!! TTTTGGTTTTGCAAGACCTG+TGG - chr2_1:11928896-11928915 Msa0195160:intron 40.0%
! ACACAGACTTTGATAACCAC+AGG + chr2_1:11928915-11928934 None:intergenic 40.0%
TGAAAGCAAAGAACTGTGGT+TGG - chr2_1:11929215-11929234 Msa0195160:intron 40.0%
TTAGGCTTGTGTAGGTTCAA+TGG + chr2_1:11929337-11929356 None:intergenic 40.0%
GTTGCTGTATAGGGTTCTTT+AGG + chr2_1:11929355-11929374 None:intergenic 40.0%
TGTTTCCGTTGAACGAATAG+TGG - chr2_1:11929378-11929397 Msa0195160:five_prime_UTR 40.0%
GTTTCCGTTGAACGAATAGT+GGG - chr2_1:11929379-11929398 Msa0195160:five_prime_UTR 40.0%
! ATGATGGAAATGTTCCCTAC+TGG - chr2_1:11929443-11929462 Msa0195160:five_prime_UTR 40.0%
TGATGGAAATGTTCCCTACT+GGG - chr2_1:11929444-11929463 Msa0195160:five_prime_UTR 40.0%
GATAACAAGTCACTTACTGG+TGG + chr2_1:11929493-11929512 None:intergenic 40.0%
! TGTTCTTCCCTTTGGCTTCT+CGG + chr2_1:11927893-11927912 None:intergenic 45.0%
TGATTACCTGAGAAGCTGAC+AGG + chr2_1:11928073-11928092 None:intergenic 45.0%
GCGGAGAAATCATTGCTAAC+AGG + chr2_1:11928393-11928412 None:intergenic 45.0%
CGCCAGAAGATCTGTGTTTA+AGG - chr2_1:11928410-11928429 Msa0195160:intron 45.0%
TGGCGTCTACGTGTTATCTT+CGG - chr2_1:11928535-11928554 Msa0195160:intron 45.0%
! ATTTGGGGTCTATGAAGCAC+TGG + chr2_1:11928581-11928600 None:intergenic 45.0%
CTGTATCTCCAATACTGCGA+CGG - chr2_1:11928730-11928749 Msa0195160:intron 45.0%
TGTATCTCCAATACTGCGAC+GGG - chr2_1:11928731-11928750 Msa0195160:intron 45.0%
ACTTAAACCCGTCGCAGTAT+TGG + chr2_1:11928741-11928760 None:intergenic 45.0%
AAGCAAAGAACTGTGGTTGG+AGG - chr2_1:11929218-11929237 Msa0195160:intron 45.0%
AAAGGGGAGACAGCAACTTT+GGG + chr2_1:11929289-11929308 None:intergenic 45.0%
TAAAGGGGAGACAGCAACTT+TGG + chr2_1:11929290-11929309 None:intergenic 45.0%
!! TGCTGTCTCCCCTTTAGAAT+TGG - chr2_1:11929294-11929313 Msa0195160:intron 45.0%
AGGGTTCTTTAGGCTTGTGT+AGG + chr2_1:11929345-11929364 None:intergenic 45.0%
TGGTCCCACTATTCGTTCAA+CGG + chr2_1:11929386-11929405 None:intergenic 45.0%
! TGAACGAATAGTGGGACCAA+AGG - chr2_1:11929387-11929406 Msa0195160:five_prime_UTR 45.0%
ACAACATGACAAGTTTCCCC+AGG + chr2_1:11929415-11929434 None:intergenic 45.0%
! TGTTCTTCCCTTTGGCTTCT+CGG + chr2_1:11927893-11927912 None:intergenic 45.0%
TGATTACCTGAGAAGCTGAC+AGG + chr2_1:11928073-11928092 None:intergenic 45.0%
GCGGAGAAATCATTGCTAAC+AGG + chr2_1:11928393-11928412 None:intergenic 45.0%
CGCCAGAAGATCTGTGTTTA+AGG - chr2_1:11928410-11928429 Msa0195160:intron 45.0%
TGGCGTCTACGTGTTATCTT+CGG - chr2_1:11928535-11928554 Msa0195160:intron 45.0%
! ATTTGGGGTCTATGAAGCAC+TGG + chr2_1:11928581-11928600 None:intergenic 45.0%
CTGTATCTCCAATACTGCGA+CGG - chr2_1:11928730-11928749 Msa0195160:intron 45.0%
TGTATCTCCAATACTGCGAC+GGG - chr2_1:11928731-11928750 Msa0195160:intron 45.0%
ACTTAAACCCGTCGCAGTAT+TGG + chr2_1:11928741-11928760 None:intergenic 45.0%
AAGCAAAGAACTGTGGTTGG+AGG - chr2_1:11929218-11929237 Msa0195160:intron 45.0%
AAAGGGGAGACAGCAACTTT+GGG + chr2_1:11929289-11929308 None:intergenic 45.0%
TAAAGGGGAGACAGCAACTT+TGG + chr2_1:11929290-11929309 None:intergenic 45.0%
!! TGCTGTCTCCCCTTTAGAAT+TGG - chr2_1:11929294-11929313 Msa0195160:intron 45.0%
AGGGTTCTTTAGGCTTGTGT+AGG + chr2_1:11929345-11929364 None:intergenic 45.0%
TGGTCCCACTATTCGTTCAA+CGG + chr2_1:11929386-11929405 None:intergenic 45.0%
! TGAACGAATAGTGGGACCAA+AGG - chr2_1:11929387-11929406 Msa0195160:five_prime_UTR 45.0%
ACAACATGACAAGTTTCCCC+AGG + chr2_1:11929415-11929434 None:intergenic 45.0%
!!! TCATATTTTTTAATTATTAT+TGG - chr2_1:11928515-11928534 Msa0195160:intron 5.0%
!!! TCATATTTTTTAATTATTAT+TGG - chr2_1:11928515-11928534 Msa0195160:intron 5.0%
GAGTGTAGTCTTCGCCAACT+TGG + chr2_1:11927871-11927890 None:intergenic 50.0%
ACTACACTCCGAGAAGCCAA+AGG - chr2_1:11927882-11927901 Msa0195160:CDS 50.0%
CTACACTCCGAGAAGCCAAA+GGG - chr2_1:11927883-11927902 Msa0195160:CDS 50.0%
CGGGTTCTTGTTCTTCCCTT+TGG + chr2_1:11927901-11927920 None:intergenic 50.0%
TTTCGAGAGAGGGAAAGGTC+GGG + chr2_1:11927920-11927939 None:intergenic 50.0%
CTTTCGAGAGAGGGAAAGGT+CGG + chr2_1:11927921-11927940 None:intergenic 50.0%
GATCCTTTCGAGAGAGGGAA+AGG + chr2_1:11927925-11927944 None:intergenic 50.0%
!! TTCGGTGTCCGTATACATGC+CGG - chr2_1:11928553-11928572 Msa0195160:intron 50.0%
TCGGGATAAATCATGGGCAC+CGG - chr2_1:11929177-11929196 Msa0195160:intron 50.0%
GGCTTGTGTAGGTTCAATGG+AGG + chr2_1:11929334-11929353 None:intergenic 50.0%
TGGAAATGTTCCCTACTGGG+AGG - chr2_1:11929447-11929466 Msa0195160:five_prime_UTR 50.0%
GGAAATGTTCCCTACTGGGA+GGG - chr2_1:11929448-11929467 Msa0195160:five_prime_UTR 50.0%
TAACTCTGTCCCTCCCAGTA+GGG + chr2_1:11929460-11929479 None:intergenic 50.0%
ATAACTCTGTCCCTCCCAGT+AGG + chr2_1:11929461-11929480 None:intergenic 50.0%
TACTGGGAGGGACAGAGTTA+TGG - chr2_1:11929460-11929479 Msa0195160:five_prime_UTR 50.0%
GAGTGTAGTCTTCGCCAACT+TGG + chr2_1:11927871-11927890 None:intergenic 50.0%
ACTACACTCCGAGAAGCCAA+AGG - chr2_1:11927882-11927901 Msa0195160:CDS 50.0%
CTACACTCCGAGAAGCCAAA+GGG - chr2_1:11927883-11927902 Msa0195160:CDS 50.0%
CGGGTTCTTGTTCTTCCCTT+TGG + chr2_1:11927901-11927920 None:intergenic 50.0%
TTTCGAGAGAGGGAAAGGTC+GGG + chr2_1:11927920-11927939 None:intergenic 50.0%
CTTTCGAGAGAGGGAAAGGT+CGG + chr2_1:11927921-11927940 None:intergenic 50.0%
GATCCTTTCGAGAGAGGGAA+AGG + chr2_1:11927925-11927944 None:intergenic 50.0%
!! TTCGGTGTCCGTATACATGC+CGG - chr2_1:11928553-11928572 Msa0195160:intron 50.0%
TCGGGATAAATCATGGGCAC+CGG - chr2_1:11929177-11929196 Msa0195160:intron 50.0%
GGCTTGTGTAGGTTCAATGG+AGG + chr2_1:11929334-11929353 None:intergenic 50.0%
TGGAAATGTTCCCTACTGGG+AGG - chr2_1:11929447-11929466 Msa0195160:five_prime_UTR 50.0%
GGAAATGTTCCCTACTGGGA+GGG - chr2_1:11929448-11929467 Msa0195160:five_prime_UTR 50.0%
TAACTCTGTCCCTCCCAGTA+GGG + chr2_1:11929460-11929479 None:intergenic 50.0%
ATAACTCTGTCCCTCCCAGT+AGG + chr2_1:11929461-11929480 None:intergenic 50.0%
TACTGGGAGGGACAGAGTTA+TGG - chr2_1:11929460-11929479 Msa0195160:five_prime_UTR 50.0%
CGACCTTTCCCTCTCTCGAA+AGG - chr2_1:11927919-11927938 Msa0195160:CDS 55.0%
GTCTCACCTGTCAGCTTCTC+AGG - chr2_1:11928064-11928083 Msa0195160:CDS 55.0%
!! CACTGGCACCGGCATGTATA+CGG + chr2_1:11928564-11928583 None:intergenic 55.0%
!! GGTCTATGAAGCACTGGCAC+CGG + chr2_1:11928575-11928594 None:intergenic 55.0%
!! ATGGGCACCGGTGTTTGCTT+TGG - chr2_1:11929189-11929208 Msa0195160:intron 55.0%
AGGTTCAATGGAGGCTCAGC+AGG + chr2_1:11929325-11929344 None:intergenic 55.0%
! ATAGTGGGACCAAAGGCTCC+TGG - chr2_1:11929394-11929413 Msa0195160:five_prime_UTR 55.0%
TAGTGGGACCAAAGGCTCCT+GGG - chr2_1:11929395-11929414 Msa0195160:five_prime_UTR 55.0%
CAAGTTTCCCCAGGAGCCTT+TGG + chr2_1:11929406-11929425 None:intergenic 55.0%
CGACCTTTCCCTCTCTCGAA+AGG - chr2_1:11927919-11927938 Msa0195160:CDS 55.0%
GTCTCACCTGTCAGCTTCTC+AGG - chr2_1:11928064-11928083 Msa0195160:CDS 55.0%
!! CACTGGCACCGGCATGTATA+CGG + chr2_1:11928564-11928583 None:intergenic 55.0%
!! GGTCTATGAAGCACTGGCAC+CGG + chr2_1:11928575-11928594 None:intergenic 55.0%
!! ATGGGCACCGGTGTTTGCTT+TGG - chr2_1:11929189-11929208 Msa0195160:intron 55.0%
AGGTTCAATGGAGGCTCAGC+AGG + chr2_1:11929325-11929344 None:intergenic 55.0%
! ATAGTGGGACCAAAGGCTCC+TGG - chr2_1:11929394-11929413 Msa0195160:five_prime_UTR 55.0%
TAGTGGGACCAAAGGCTCCT+GGG - chr2_1:11929395-11929414 Msa0195160:five_prime_UTR 55.0%
CAAGTTTCCCCAGGAGCCTT+TGG + chr2_1:11929406-11929425 None:intergenic 55.0%
AGTGGGACCAAAGGCTCCTG+GGG - chr2_1:11929396-11929415 Msa0195160:five_prime_UTR 60.0%
AGTGGGACCAAAGGCTCCTG+GGG - chr2_1:11929396-11929415 Msa0195160:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 11927861 11929522 11927861 ID=Msa0195160;Name=Msa0195160
chr2_1 mRNA 11927861 11929522 11927861 ID=Msa0195160-mRNA-1;Parent=Msa0195160;Name=Msa0195160-mRNA-1;_AED=0.22;_eAED=0.22;_QI=201|1|0.66|1|1|1|3|0|147
chr2_1 exon 11927861 11928223 11927861 ID=Msa0195160-mRNA-1:exon:5225;Parent=Msa0195160-mRNA-1
chr2_1 exon 11928952 11929008 11928952 ID=Msa0195160-mRNA-1:exon:5224;Parent=Msa0195160-mRNA-1
chr2_1 exon 11929298 11929522 11929298 ID=Msa0195160-mRNA-1:exon:5223;Parent=Msa0195160-mRNA-1
chr2_1 five_prime_UTR 11929322 11929522 11929322 ID=Msa0195160-mRNA-1:five_prime_utr;Parent=Msa0195160-mRNA-1
chr2_1 CDS 11929298 11929321 11929298 ID=Msa0195160-mRNA-1:cds;Parent=Msa0195160-mRNA-1
chr2_1 CDS 11928952 11929008 11928952 ID=Msa0195160-mRNA-1:cds;Parent=Msa0195160-mRNA-1
chr2_1 CDS 11927861 11928223 11927861 ID=Msa0195160-mRNA-1:cds;Parent=Msa0195160-mRNA-1
Gene Sequence

>Msa0195160

ATGTCTCACCTGTCAGCTTCTCAGATGGCTGTTACTGTTCCTGTTAGCAATGATTTCTCCGCCAGAAGATCTGTGTTTAAGGCATCTAACATAAACTTTCGGGATAAATCATGGGCACCGGTGTTTGCTTTGGATATGAAAGCAAAGAACTGTGGTTGGAGGAGAAATCAGAATGTGATTTGCATGTCAGTGCAACAAGCAAGTGTGCCCAAAGTTGCTGTCTCCCCTTTAGAATTGGAAAATCCTGCTGAGCCTCCATTGAACCTACACAAGCCTAAAGAACCCTATACAGCAACAATTGTTTCCGTTGAACGAATAGTGGGACCAAAGGCTCCTGGGGAAACTTGTCATGTTGTGATCAATCATGATGGAAATGTTCCCTACTGGGAGGGACAGAGTTATGGTGTTATTCCACCAGTAAGTGACTTGTTATCCAAATACTGA
Protein sequence

>Msa0195160

MSHLSASQMAVTVPVSNDFSARRSVFKASNINFRDKSWAPVFALDMKAKNCGWRRNQNVICMSVQQASVPKVAVSPLELENPAEPPLNLHKPKEPYTATIVSVERIVGPKAPGETCHVVINHDGNVPYWEGQSYGVIPPVSDLLSKY*