AlfalfaGEDB | Msa0228330

M. sativa cultivar zhongmu-4 / Msa0228330

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0228330	XP_039688996.1	98.143	700	13	0	1	700	1	700	0.0	1422

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0228330	sp\|Q6R2K1\|SRF5_ARATH	61.135	705	259	8	3	699	1	698	0.0	848

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0228330	A0A396I0G6	98.143	700	13	0	1	700	1	700	0.0	1422

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0049640	Msa0228330	0.878904	1.925139e-69	-8.615850e-47
Msa0228330	Msa0577930	0.910918	1.093861e-82	-8.615850e-47
Msa0228330	Msa0625520	0.943605	8.898890e-103	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0228330	MtrunA17_Chr4g0000081	98.143	700	13	0	1	700	1	700	0.0	1422
Msa0228330	MtrunA17_Chr8g0345471	48.968	727	316	15	9	700	6	712	0.0	634
Msa0228330	MtrunA17_Chr2g0289161	44.633	708	334	8	25	699	41	723	0.0	577
Msa0228330	MtrunA17_Chr2g0281501	38.089	722	361	15	12	675	17	710	7.00e-134	411
Msa0228330	MtrunA17_Chr7g0258451	33.091	689	332	15	25	680	27	619	8.71e-110	347
Msa0228330	MtrunA17_Chr2g0323941	52.244	312	136	6	382	682	463	772	8.97e-98	318
Msa0228330	MtrunA17_Chr2g0323941	41.833	251	140	3	10	255	23	272	5.09e-53	196
Msa0228330	MtrunA17_Chr4g0022431	52.685	298	132	4	395	684	541	837	1.04e-95	314
Msa0228330	MtrunA17_Chr4g0022431	47.210	233	118	2	10	242	20	247	2.22e-54	200
Msa0228330	MtrunA17_Chr1g0174841	42.724	323	159	5	382	680	50	370	9.66e-78	254

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0228330	AT1G78980.1	61.135	705	259	8	3	699	1	698	0.0	848
Msa0228330	AT3G13065.1	56.745	682	264	8	16	678	18	687	0.0	729
Msa0228330	AT3G14350.1	49.501	701	320	10	10	686	12	702	0.0	616
Msa0228330	AT4G22130.1	45.734	715	332	10	10	699	14	697	0.0	605
Msa0228330	AT3G14350.2	50.000	674	305	9	36	686	1	665	0.0	601
Msa0228330	AT1G53730.1	48.592	710	319	12	21	698	23	718	0.0	593
Msa0228330	AT1G53730.2	48.664	711	318	13	21	698	23	719	0.0	590
Msa0228330	AT3G14350.3	46.571	700	314	10	10	686	12	674	0.0	554
Msa0228330	AT4G22130.2	44.123	587	272	10	138	699	1	556	2.59e-154	459
Msa0228330	AT5G06820.1	38.235	714	390	12	1	680	1	697	4.72e-146	443

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Find 178 sgRNAs with CRISPR-Local

Find 347 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCATCAGTTAGTGTT+AGG	0.400543	2_1:+80252278	Msa0228330:CDS
AAACCATAGTCTGAGAGACG+GGG	0.706019	2_1:-80253416	None:intergenic
AAACCCTTCACTTATTGATG+AGG	0.565240	2_1:+80252781	Msa0228330:CDS
AAAGAACTGGAGGAAACTCT+TGG	0.490980	2_1:+80251214	Msa0228330:intron
AAAGCACTTTACCTGTCTAG+AGG	0.655071	2_1:-80253903	None:intergenic
AACAGAAATTTATCTGATTT+GGG	0.306116	2_1:+80249585	Msa0228330:intron
AACCCTCATCAATAAGTGAA+GGG	0.612284	2_1:-80252784	None:intergenic
AACCCTTCACTTATTGATGA+GGG	0.529499	2_1:+80252782	Msa0228330:CDS
AACTGTATACATCGCTCTTC+AGG	0.241368	2_1:-80253842	None:intergenic
AAGAGCGATGTATACAGTTT+TGG	0.282071	2_1:+80253846	Msa0228330:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGGTTTGTATTTGAGTATA+TGG	-	chr2_1:80249672-80249691	None:intergenic	30.0%
TGAGAAATGAAAAAGAAAGG+AGG	-	chr2_1:80249691-80249710	None:intergenic	30.0%
TTTGAGCAACAACAATTTCA+AGG	+	chr2_1:80249775-80249794	Msa0228330:CDS	30.0%
TTGAGCAACAACAATTTCAA+GGG	+	chr2_1:80249776-80249795	Msa0228330:CDS	30.0%
CATTCTTAATCAACCATGTT+TGG	+	chr2_1:80250008-80250027	Msa0228330:intron	30.0%
AAAGGTGATAAATCCAAACA+TGG	-	chr2_1:80250024-80250043	None:intergenic	30.0%
AATGGTCCTTCTTTGTATTT+AGG	+	chr2_1:80250506-80250525	Msa0228330:intron	30.0%
AAGCTTTCCTCTTTCTTATT+TGG	+	chr2_1:80250558-80250577	Msa0228330:intron	30.0%
TCATTTCTTACATCTTCTTG+AGG	-	chr2_1:80250700-80250719	None:intergenic	30.0%
CTGCAAAACAATCAATTATC+TGG	+	chr2_1:80250803-80250822	Msa0228330:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	80249191	80254411	80249191	ID=Msa0228330;Name=Msa0228330
chr2_1	mRNA	80249191	80254411	80249191	ID=Msa0228330-mRNA-1;Parent=Msa0228330;Name=Msa0228330-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|1\|1\|1\|16\|0\|700
chr2_1	exon	80249191	80249278	80249191	ID=Msa0228330-mRNA-1:exon:24327;Parent=Msa0228330-mRNA-1
chr2_1	exon	80249380	80249512	80249380	ID=Msa0228330-mRNA-1:exon:24328;Parent=Msa0228330-mRNA-1
chr2_1	exon	80249590	80249661	80249590	ID=Msa0228330-mRNA-1:exon:24329;Parent=Msa0228330-mRNA-1
chr2_1	exon	80249772	80249837	80249772	ID=Msa0228330-mRNA-1:exon:24330;Parent=Msa0228330-mRNA-1
chr2_1	exon	80250165	80250236	80250165	ID=Msa0228330-mRNA-1:exon:24331;Parent=Msa0228330-mRNA-1
chr2_1	exon	80250331	80250402	80250331	ID=Msa0228330-mRNA-1:exon:24332;Parent=Msa0228330-mRNA-1
chr2_1	exon	80250637	80250708	80250637	ID=Msa0228330-mRNA-1:exon:24333;Parent=Msa0228330-mRNA-1
chr2_1	exon	80250799	80250864	80250799	ID=Msa0228330-mRNA-1:exon:24334;Parent=Msa0228330-mRNA-1
chr2_1	exon	80251071	80251136	80251071	ID=Msa0228330-mRNA-1:exon:24335;Parent=Msa0228330-mRNA-1
chr2_1	exon	80251218	80251528	80251218	ID=Msa0228330-mRNA-1:exon:24336;Parent=Msa0228330-mRNA-1
chr2_1	exon	80252230	80252501	80252230	ID=Msa0228330-mRNA-1:exon:24337;Parent=Msa0228330-mRNA-1
chr2_1	exon	80252761	80253029	80252761	ID=Msa0228330-mRNA-1:exon:24338;Parent=Msa0228330-mRNA-1
chr2_1	exon	80253334	80253454	80253334	ID=Msa0228330-mRNA-1:exon:24339;Parent=Msa0228330-mRNA-1
chr2_1	exon	80253777	80253913	80253777	ID=Msa0228330-mRNA-1:exon:24340;Parent=Msa0228330-mRNA-1
chr2_1	exon	80254034	80254190	80254034	ID=Msa0228330-mRNA-1:exon:24341;Parent=Msa0228330-mRNA-1
chr2_1	exon	80254283	80254411	80254283	ID=Msa0228330-mRNA-1:exon:24342;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80249191	80249278	80249191	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80249380	80249512	80249380	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80249590	80249661	80249590	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80249772	80249837	80249772	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80250165	80250236	80250165	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80250331	80250402	80250331	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80250637	80250708	80250637	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80250799	80250864	80250799	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80251071	80251136	80251071	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80251218	80251528	80251218	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80252230	80252501	80252230	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80252761	80253029	80252761	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80253334	80253454	80253334	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80253777	80253913	80253777	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80254034	80254190	80254034	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1
chr2_1	CDS	80254283	80254411	80254283	ID=Msa0228330-mRNA-1:cds;Parent=Msa0228330-mRNA-1

Gene Sequence

>Msa0228330

ATGCACCTCAACCACAACCTCTCGTGGTTGTGGATTATTGTTTTGATCTCAACCGCCTCAGTACAATGCAAAACCTCCTCCCAAGATGTTTCAGCCCTTAATGTAATGTACACCAGCTTGAATTCTCCTTCTCAACTCTCTGGCTGGAAATCAAGCGGAGGTGATCCTTGTGGTGAAAATTGGGAAGGAATCAAATGCTCTGGCTCATCTGTCACAGAAATAAATTTATCTGATTTGGGACTTAGTGGATCATTGGGCTACCAGTTATCACAGTTAACATCTGTTACCGACTTTGATTTGAGCAACAACAATTTCAAGGGTGACATACCGTATCAACTCCCTCCAAATGCTCGTAACGTAGATCTTTCTAAGAATGCGTTCACTGGAAACATACCTTACTCCATTGGTCAGATGAAAGAACTAACTTCTCTAAATCTTGCACATAATAAGCTCAATAACCAGCTGGGGGATATGTTTACGAGCCTCACTAAACTCAAACAATTGGATGTATCATTCAATTCACTGTCAGGAGAACTGCCTCAGAGTTTGAAATCAGCAACAAGCCTCAAGAAGATACATCTGCAAAACAATCAATTATCTGGTTCAATAAATGTCCTTGCCTACCCTCCACTTGACGATGTGAATGTTGAGAACAACAAATTTACTGGTTGGATTCCTGAGGAGTTAAAGGATATAAATAGTTTGCAAACTGGAGGAAACTCTTGGAAAACTGGGCCAGCACCACCTCCTCCTCCCGGTACACCTCCTATCAAGCACCATTCCGAGGAAAAGGAAGGAAAATCATCAAATTCTCTTGTTACTGGATTGATTATAGCTGGTATTGCTTTTGGTGCGCTGGTCGTAATCATACTCGTTGCTGCTCTTTTTAAAAGAAAGACACCGTATGGTTCTTCACGTTTTATTGATGAAGAGAGGCGTAGCCAGCACAGATCCTTTACACCCCTTGCATCTCAGGAGTTGACTAAAGATATAGGTGCTAATGACACAGAATACAAAGGATTTAAGGCAATGGATTCGACTTCAATTGACATAATGGCTTTACAAAAAAATCCATCAGTTAGTGTTAGGTCATCAGTCTCTGATTGTGTCCAAAACTTTAATGACAATGAGTTTGCAAATCGTCTAAATTCCAGAAAAAGCGCATCGATCCGCTGTACTGCTTTTTCATTGGGGGAGTTGCAAACTGGTACTGCTAACTTTGCTTCAGGCCGACTTCTTGGTGAAGGATCCATTGGACCTGTTTATCGTGCCAAATATGCTGATGGAAAGGTACTGGCTGTAAAAAAATTAAACCCTTCACTTATTGATGAGGGTTCTCCTGAGGAATTCACTCAGATTCTATCAAGCATGTGCAAACTTCGTCATCCAAATATTGCCGAGCTTGTTGGCTATTGTTCAGAACAGGGGCACATGTTGGTATATGAGTATTTCAGAAATGGTTCTCTGCACGACTTTTTACACTTATCAGATGACTTCAGCAAACCACTAACTTGGAACACCAGAGTCAGAATCGCATTGGGCACTGCTCGGGCTGTTGAGTACCTACATGAAGCTTGTTCTCCACCTGTACTTCACAAGAATATCAAATCCGCCAATATTTTGCTTGACACAGATTTGAATCCCCGTCTCTCAGACTATGGTTTGGCATCCTTCCACCAGCGTACTAGCCAAAATCTTGGGGCAGGATATAATGCACCCGAATGCACAAAACCATCAGCCTATACCCTGAAGAGCGATGTATACAGTTTTGGGGTAGTGATGCTCGAACTTTTTACAGGTCGAATGCCTCTAGACAGTTCAAAGCCAAAATCAGAGCAAAACCTCGTTCGTTGGGCTACGCCACAGCTTCGTGACATAAACGCTGTGGAAAAGATGGTAGACCCTGCATTGCGGGGACTTTACCCTCCTAAATCACTCTTTCGATTTGCTGACATCGTCGCCCTTTGTGTTCAGTCTGAGCCCGAATTTCGGCCATCTGCATCAGAGGTGGTTCAGGCATTGGTGCGATTAGTTCAACGTTCAAGTTTGAAAATGAGAGAGGATCTTGGTGCATGTGGCCGGACAGATGATTATGATGATTGA

Protein sequence

>Msa0228330

MHLNHNLSWLWIIVLISTASVQCKTSSQDVSALNVMYTSLNSPSQLSGWKSSGGDPCGENWEGIKCSGSSVTEINLSDLGLSGSLGYQLSQLTSVTDFDLSNNNFKGDIPYQLPPNARNVDLSKNAFTGNIPYSIGQMKELTSLNLAHNKLNNQLGDMFTSLTKLKQLDVSFNSLSGELPQSLKSATSLKKIHLQNNQLSGSINVLAYPPLDDVNVENNKFTGWIPEELKDINSLQTGGNSWKTGPAPPPPPGTPPIKHHSEEKEGKSSNSLVTGLIIAGIAFGALVVIILVAALFKRKTPYGSSRFIDEERRSQHRSFTPLASQELTKDIGANDTEYKGFKAMDSTSIDIMALQKNPSVSVRSSVSDCVQNFNDNEFANRLNSRKSASIRCTAFSLGELQTGTANFASGRLLGEGSIGPVYRAKYADGKVLAVKKLNPSLIDEGSPEEFTQILSSMCKLRHPNIAELVGYCSEQGHMLVYEYFRNGSLHDFLHLSDDFSKPLTWNTRVRIALGTARAVEYLHEACSPPVLHKNIKSANILLDTDLNPRLSDYGLASFHQRTSQNLGAGYNAPECTKPSAYTLKSDVYSFGVVMLELFTGRMPLDSSKPKSEQNLVRWATPQLRDINAVEKMVDPALRGLYPPKSLFRFADIVALCVQSEPEFRPSASEVVQALVRLVQRSSLKMREDLGACGRTDDYDD*