AlfalfaGEDB | Msa0229650

M. sativa cultivar zhongmu-4 / Msa0229650

KEGG: -
GO: -
PFAM: PF13947.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0229650	RHN77992.1	68.266	271	55	9	4	249	2	266	8.53e-108	335

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0229650	A0A396JKW1	68.266	271	55	9	4	249	2	266	4.07e-108	335

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0229650	Msa0399300	0.812893	3.370703e-51	-8.615850e-47
Msa0229650	Msa0626280	0.808778	2.620281e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0229650	MtrunA17_Chr1g0160711	68.266	271	55	9	4	249	2	266	7.84e-112	335
Msa0229650	MtrunA17_Chr1g0160761	65.299	268	63	5	1	241	1	265	2.44e-105	319
Msa0229650	MtrunA17_Chr1g0160661	64.151	265	69	7	1	246	27	284	3.81e-103	308
Msa0229650	MtrunA17_Chr1g0160721	61.200	250	75	6	1	235	12	254	4.60e-89	277
Msa0229650	MtrunA17_Chr1g0160701	59.740	231	69	6	18	227	33	260	5.04e-77	246
Msa0229650	MtrunA17_Chr1g0160561	51.181	254	100	6	3	232	7	260	1.67e-67	221
Msa0229650	MtrunA17_Chr1g0158061	36.019	211	103	9	33	217	35	239	2.80e-25	101
Msa0229650	MtrunA17_Chr6g0467711	31.200	250	123	11	2	217	13	247	5.40e-25	103
Msa0229650	MtrunA17_Chr1g0158411	30.120	249	127	9	2	216	15	250	2.88e-24	101
Msa0229650	MtrunA17_Chr1g0157791	32.877	219	117	7	23	217	28	240	4.33e-24	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 47 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCGATATACACAGAA+AGG	0.584628	2_2:-1416946	None:intergenic
AAAGACAGTCCAGAAGAATG+TGG	0.689791	2_2:+1416394	Msa0229650:CDS
AACACGAGTCATGTTCAAGA+AGG	0.638813	2_2:+1416335	Msa0229650:CDS
AACCATAGTACAAAATCAAT+TGG	0.434683	2_2:-1416453	None:intergenic
ACAAATCTCTGTCAGAATTC+GGG	0.418168	2_2:+1416746	Msa0229650:CDS
AGTTTATGTATCTCACTTCT+TGG	0.350053	2_2:+1416791	Msa0229650:CDS
ATAACATCAGCCACCCTTTC+CGG	0.297512	2_2:+1416368	Msa0229650:CDS
ATAGACATCTTGATTCGCTT+AGG	0.529424	2_2:+1417145	Msa0229650:CDS
ATAGTCGGTGTAAACATGGT+CGG	0.679615	2_2:+1416896	Msa0229650:CDS
ATCCATAAAACATCATATGA+CGG	0.575198	2_2:-1416855	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGAAATCCAATAGTCGTA+TGG	-	chr2_2:1416524-1416543	None:intergenic	30.0%
GAATATTCCTATATCGACAA+AGG	+	chr2_2:1416658-1416677	Msa0229650:CDS	30.0%
GAAAATTCTTTCTATGTTGG+TGG	+	chr2_2:1416718-1416737	Msa0229650:CDS	30.0%
TACAAATCTCTGTCAGAATT+CGG	+	chr2_2:1416745-1416764	Msa0229650:CDS	30.0%
AGTTTATGTATCTCACTTCT+TGG	+	chr2_2:1416791-1416810	Msa0229650:CDS	30.0%
GTTTATGTATCTCACTTCTT+GGG	+	chr2_2:1416792-1416811	Msa0229650:CDS	30.0%
TTCATATGGATTCTACCATT+TGG	+	chr2_2:1417019-1417038	Msa0229650:intron	30.0%
TATATAGACCCATTTCCAAA+TGG	-	chr2_2:1417037-1417056	None:intergenic	30.0%
ATTGAAATCCAATAGTCGTA+TGG	-	chr2_2:1416524-1416543	None:intergenic	30.0%
GAATATTCCTATATCGACAA+AGG	+	chr2_2:1416658-1416677	Msa0229650:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	1416262	1417219	1416262	ID=Msa0229650;Name=Msa0229650
chr2_2	mRNA	1416262	1417219	1416262	ID=Msa0229650-mRNA-1;Parent=Msa0229650;Name=Msa0229650-mRNA-1;_AED=0.06;_eAED=0.07;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|265
chr2_2	exon	1416262	1416964	1416262	ID=Msa0229650-mRNA-1:exon:475;Parent=Msa0229650-mRNA-1
chr2_2	exon	1417125	1417219	1417125	ID=Msa0229650-mRNA-1:exon:476;Parent=Msa0229650-mRNA-1
chr2_2	CDS	1416262	1416964	1416262	ID=Msa0229650-mRNA-1:cds;Parent=Msa0229650-mRNA-1
chr2_2	CDS	1417125	1417219	1417125	ID=Msa0229650-mRNA-1:cds;Parent=Msa0229650-mRNA-1

Gene Sequence

>Msa0229650

ATGCTTTCCTTAACGTCCTTTGTTATGGTGGCAATAGTGTTACTAATGAGCAACAAAGTGTATGGTGATGAACAACACGAGTCATGTTCAAGAAGGTGCGGTGTTCATAACATCAGCCACCCTTTCCGGCTAAAAGACAGTCCAGAAGAATGTGGTGACAAGAGGTACAACCTATCATGTGAGGACAATAACCAATTGATTTTGTACTATGGTTCATTTGGAAAATACTATGTACAATCAATTAATTACAACAACTTCACCATACGACTATTGGATTTCAATCTTGCTTTCAGTTCTAATTTCATCACACCCTACCTAGCTTTTAATCGATACGAAAACTACAATCGTCATATATTGACGAAATCTATGTTGTATGTGAGCTGTCCAAATCGGTCGGAATATTCCTATATCGACAAAGGTGGTAGCTGTATGAATAGTAGTTCATATTCACAGTACGAAAATTCTTTCTATGTTGGTGGCTACTACAAATCTCTGTCAGAATTCGGGCTGGGAGACGGGTGTCGTATACAGTTTATGTATCTCACTTCTTGGGATGTTGAAGATGACATAAATAACATTTCTTGCTCGGACTTCCGTCATATGATGTTTTATGGATTTGAATTTTCTTGGCTCAATAGTCGGTGTAAACATGGTCGGTACGCGAAGCTCGATCAATATAACCAGCCTTTCTGTGTATATCGAGGCATGTCTGCTTGGGAGGTCATAGACATCTTGATTCGCTTAGGTAAGTCCTCACATTGTTTTAAGTCAATGCAAGACATGCTAAACAAAGTGTGA

Protein sequence

>Msa0229650

MLSLTSFVMVAIVLLMSNKVYGDEQHESCSRRCGVHNISHPFRLKDSPEECGDKRYNLSCEDNNQLILYYGSFGKYYVQSINYNNFTIRLLDFNLAFSSNFITPYLAFNRYENYNRHILTKSMLYVSCPNRSEYSYIDKGGSCMNSSSYSQYENSFYVGGYYKSLSEFGLGDGCRIQFMYLTSWDVEDDINNISCSDFRHMMFYGFEFSWLNSRCKHGRYAKLDQYNQPFCVYRGMSAWEVIDILIRLGKSSHCFKSMQDMLNKV*