AlfalfaGEDB | Msa0234500

M. sativa cultivar zhongmu-4 / Msa0234500

KEGG: K04730|K04733
GO: GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234500	XP_003593653.1	93.824	340	21	0	1	340	1	340	0.0	649

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234500	sp\|Q9LX29\|ACR4L_ARATH	36.513	304	173	6	40	329	489	786	7.68e-48	176

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234500	A2Q519	93.824	340	21	0	1	340	1	340	0.0	649

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0193400	Msa0234500	0.985477	1.089331e-163	-8.615850e-47
Msa0234500	Msa0272870	0.851232	1.014484e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234500	MtrunA17_Chr2g0282861	93.824	340	21	0	1	340	1	340	0.0	649
Msa0234500	MtrunA17_Chr4g0034931	35.593	295	168	6	49	329	126	412	2.06e-47	166
Msa0234500	MtrunA17_Chr3g0137191	35.640	289	158	8	56	328	59	335	1.19e-46	162
Msa0234500	MtrunA17_Chr2g0329401	31.579	361	203	6	7	328	440	795	1.22e-45	166
Msa0234500	MtrunA17_Chr7g0271451	34.848	330	160	9	45	332	154	470	2.81e-45	161
Msa0234500	MtrunA17_Chr1g0213991	33.746	323	189	7	35	340	69	383	9.16e-45	159
Msa0234500	MtrunA17_Chr4g0033571	36.184	304	162	7	48	328	498	792	1.21e-44	163
Msa0234500	MtrunA17_Chr3g0123141	34.868	304	169	8	44	330	589	880	2.44e-44	162
Msa0234500	MtrunA17_Chr2g0333181	34.915	295	168	6	49	328	26	311	3.51e-44	155
Msa0234500	MtrunA17_Chr6g0485721	34.375	288	159	7	60	329	95	370	7.67e-44	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234500	AT5G46080.1	55.000	340	137	6	1	334	1	330	7.34e-112	328
Msa0234500	AT3G59420.1	36.513	304	173	6	40	329	489	786	7.81e-49	176
Msa0234500	AT4G22130.2	34.680	297	173	5	47	328	238	528	7.93e-47	167
Msa0234500	AT4G22130.1	34.680	297	173	5	47	328	379	669	4.81e-46	166
Msa0234500	AT1G09440.1	34.899	298	168	6	51	332	145	432	6.09e-45	160
Msa0234500	AT1G09440.2	34.899	298	168	6	51	332	145	432	6.09e-45	160
Msa0234500	AT5G11020.3	35.540	287	163	6	55	328	72	349	8.78e-45	157
Msa0234500	AT5G11020.1	35.540	287	163	6	55	328	133	410	9.37e-45	159
Msa0234500	AT5G11020.4	35.540	287	163	6	55	328	95	372	1.20e-44	157
Msa0234500	AT1G61860.1	33.333	330	193	9	30	340	50	371	1.26e-44	157

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Find 72 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAATGAATGAGAAAA+AGG	0.280233	2_2:-7270226	None:intergenic
AAAGTGATGTTTATGGATTA+GGG	0.259255	2_2:+7271113	Msa0234500:CDS
AAAGTTGCTTCAGCTTGTGT+TGG	0.541984	2_2:+7271289	Msa0234500:CDS
AAATATAGACAAGTTCTATG+TGG	0.617232	2_2:-7271519	None:intergenic
AAGAGGTTTAAGATTGCAGC+TGG	0.475084	2_2:+7270872	Msa0234500:CDS
AAGTGATGTTTATGGATTAG+GGG	0.608641	2_2:+7271114	Msa0234500:CDS
AATCTCATACCCTTAATCAA+AGG	0.575187	2_2:-7271202	None:intergenic
AATGAAGGAATTAGAGGTGG+TGG	0.614840	2_2:+7271079	Msa0234500:CDS
ACAATAGCATTCATATTAGA+AGG	0.378832	2_2:-7271256	None:intergenic
ACACAACCATGTACAATATT+TGG	0.145400	2_2:-7270935	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTATATACATGTGACTTTGT+TGG	-	chr2_2:7270178-7270197	None:intergenic	30.0%
TATTGCTTATGACATAGTCA+TGG	+	chr2_2:7270286-7270305	Msa0234500:CDS	30.0%
GCAACATCAATAACTGTTAA+AGG	-	chr2_2:7270425-7270444	None:intergenic	30.0%
GTTATTGATGTTGCTACAAA+TGG	+	chr2_2:7270431-7270450	Msa0234500:CDS	30.0%
GAGAATATGAATTACATCGA+GGG	-	chr2_2:7270741-7270760	None:intergenic	30.0%
AGAGAATATGAATTACATCG+AGG	-	chr2_2:7270742-7270761	None:intergenic	30.0%
TACTTGCATGTAAATCATGA+TGG	+	chr2_2:7270821-7270840	Msa0234500:CDS	30.0%
ACACAACCATGTACAATATT+TGG	-	chr2_2:7270938-7270957	None:intergenic	30.0%
GTAGAAAAGAGAGGATTAAT+TGG	+	chr2_2:7271034-7271053	Msa0234500:CDS	30.0%
GATGATGAATATTGGAATGA+AGG	+	chr2_2:7271064-7271083	Msa0234500:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	7270149	7271557	7270149	ID=Msa0234500;Name=Msa0234500
chr2_2	mRNA	7270149	7271557	7270149	ID=Msa0234500-mRNA-1;Parent=Msa0234500;Name=Msa0234500-mRNA-1;_AED=0.04;_eAED=0.04;_QI=120\|1\|1\|1\|1\|1\|2\|158\|340
chr2_2	exon	7270149	7270662	7270149	ID=Msa0234500-mRNA-1:exon:3094;Parent=Msa0234500-mRNA-1
chr2_2	exon	7270771	7271557	7270771	ID=Msa0234500-mRNA-1:exon:3095;Parent=Msa0234500-mRNA-1
chr2_2	five_prime_UTR	7270149	7270268	7270149	ID=Msa0234500-mRNA-1:five_prime_utr;Parent=Msa0234500-mRNA-1
chr2_2	CDS	7270269	7270662	7270269	ID=Msa0234500-mRNA-1:cds;Parent=Msa0234500-mRNA-1
chr2_2	CDS	7270771	7271399	7270771	ID=Msa0234500-mRNA-1:cds;Parent=Msa0234500-mRNA-1
chr2_2	three_prime_UTR	7271400	7271557	7271400	ID=Msa0234500-mRNA-1:three_prime_utr;Parent=Msa0234500-mRNA-1

Gene Sequence

>Msa0234500

ATGAACCTTGGGGAACATATTGCTTATGACATAGTCATGGCAATTCTATCAATATCTCTCATAATTCTTGGTATTTTCCTCTTTCTCGCTTGCAAAAAGAAACCAGTTGAATCAGAAGAAACACTTCCACTGAAACACAGTGCTCGTGCATACCCTTTAACAGTTATTGATGTTGCTACAAATGGCTTCAACCATAGGAGAATCATTGGAAAGGGTCGTTTAGGAACAGTTTATGCTGGAAAACTCGAAAACGAAGAGCTTGTAGCTGTGAAACGCATTCATTCTGTTCTTGTGTTAAGCAACGCAGGATTTGGTTTTTCATCTGTGATGAAATGGCTCTCTTCAGCTCAACACCCTAACGTTGTTCCCATAATAGGATTCTCAGAAGCACCAGGTGAAAGAGTTATACTGATGGAGTTTGTTCGAATGGCGAATTTGGAATTTTACTTGCATGTAAATCATGATGGTGGTTCTTCTTCACTTTTGGATTGGAATAAGAGGTTTAAGATTGCAGCTGGAGTAGCTAAAGGGCTACAATATCTTCATGAAGTGGTAGCACCAAATATTGTACATGGTTGTGTTAAATCTTCTAATGTTCTTATTGATGTTAATTTTTGTGCTAAAATTTCTGATTATGGACTTAGTTTTTTGGGGGGTGTAGAAAAGAGAGGATTAATTGGTTATGTAGATGATGAATATTGGAATGAAGGAATTAGAGGTGGTGGTGTTTGTAAGGAAAGTGATGTTTATGGATTAGGGGTGATTTTGTTGGAGCTTTTAAGTGGAAGAGGGTGTGAGGGTGGATTATTGGTGAAATGGGCTTTGCCTTTGATTAAGGGTATGAGATTTAGTGAAGTTTTGGATCCTAGGTTGATTATTCCTTCTAATATGAATGCTATTGTTAGATTAGCAAAAGTTGCTTCAGCTTGTGTTGGTAATTCAAGGAAATGTAGACCTTGTGTGAACCATGTTGTGAACATTTTGAATGATTTAGAGATGGAAGTGTGTCTCTTATCAAATTGA

Protein sequence

>Msa0234500

MNLGEHIAYDIVMAILSISLIILGIFLFLACKKKPVESEETLPLKHSARAYPLTVIDVATNGFNHRRIIGKGRLGTVYAGKLENEELVAVKRIHSVLVLSNAGFGFSSVMKWLSSAQHPNVVPIIGFSEAPGERVILMEFVRMANLEFYLHVNHDGGSSSLLDWNKRFKIAAGVAKGLQYLHEVVAPNIVHGCVKSSNVLIDVNFCAKISDYGLSFLGGVEKRGLIGYVDDEYWNEGIRGGGVCKESDVYGLGVILLELLSGRGCEGGLLVKWALPLIKGMRFSEVLDPRLIIPSNMNAIVRLAKVASACVGNSRKCRPCVNHVVNILNDLEMEVCLLSN*