AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0242610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242610 MtrunA17_Chr2g0294861 97.348 264 7 0 1 264 1 264 0.0 528
Msa0242610 MtrunA17_Chr2g0294871 82.114 246 39 4 1 245 1 242 2.97e-140 394
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242610 AT5G49320.1 60.241 249 85 5 2 248 8 244 7.39e-101 295

Find 55 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCACCTTCTTTAATTAAT+TGG 0.027243 2_2:+18927764 Msa0242610:CDS
ATGCTGTAGCAATTTGTTCT+TGG 0.243620 2_2:-18928177 None:intergenic
ATCGATAAGTATTCATGTTC+AGG 0.276126 2_2:-18928761 None:intergenic
TAGAGTATAGTTTGATCCTT+TGG 0.294620 2_2:+18928500 Msa0242610:three_prime_UTR
CTTTGACTTTGAGGTGATCT+TGG 0.295999 2_2:-18928360 None:intergenic
GATCCTTTGGTTTGGTCAAA+TGG 0.301043 2_2:+18928513 Msa0242610:three_prime_UTR
ATCACCTTCTTTAATTAATT+GGG 0.320584 2_2:+18927765 Msa0242610:CDS
GGTCCACTTGTCTGTAACTT+GGG 0.330573 2_2:+18928716 Msa0242610:three_prime_UTR
ACTAGCAATTGCTTTGGTTA+TGG 0.333310 2_2:+18927708 Msa0242610:CDS
TTAGGAATTGTCTCTCTTGA+AGG 0.366881 2_2:+18927135 Msa0242610:intron
TGGTCCACTTGTCTGTAACT+TGG 0.386376 2_2:+18928715 Msa0242610:three_prime_UTR
ATCCTTTGGTTTGGTCAAAT+GGG 0.391559 2_2:+18928514 Msa0242610:three_prime_UTR
GATTTAGCAGAAGGAGAATC+AGG 0.397735 2_2:-18927790 None:intergenic
GCAATATGTCTTTGACTTTG+AGG 0.410164 2_2:-18928369 None:intergenic
TGTTGGACTAGCAATTGCTT+TGG 0.417090 2_2:+18927702 Msa0242610:CDS
GAAGGTTTGTTCTCAGCTGC+AGG 0.422199 2_2:+18928204 Msa0242610:CDS
AATGTGTATACAATAGTAAT+GGG 0.423786 2_2:+18927647 Msa0242610:CDS
ACAGATTTGAGTAGCAAAAC+AGG 0.437995 2_2:+18927210 Msa0242610:CDS
AAAACCAGCCAACCATGTTA+AGG 0.443331 2_2:-18927812 None:intergenic
AAAACTTGTGAGTTTAGATC+GGG 0.443804 2_2:+18928691 Msa0242610:three_prime_UTR
TTTGTGCACGAAGTGCTGTT+AGG 0.449903 2_2:-18928269 None:intergenic
TACCTCGGAACGTGTGGCTT+CGG 0.452781 2_2:-18927295 None:intergenic
TATAGTTTGATCCTTTGGTT+TGG 0.455783 2_2:+18928505 Msa0242610:three_prime_UTR
TTTGACTCTCAATTGTTGTA+GGG 0.459330 2_2:-18928410 None:intergenic
TGCTAAATCCTTAACATGGT+TGG 0.463393 2_2:+18927804 Msa0242610:CDS
GAATGTGTATACAATAGTAA+TGG 0.474577 2_2:+18927646 Msa0242610:CDS
AAATCCTTAACATGGTTGGC+TGG 0.479778 2_2:+18927808 Msa0242610:CDS
GTGACATGGATGAATACAAA+TGG 0.480357 2_2:+18927601 Msa0242610:CDS
AATACAAATGGAAAAGTAAA+GGG 0.480387 2_2:+18927613 Msa0242610:CDS
TGACTTTGAGGTGATCTTGG+TGG 0.482477 2_2:-18928357 None:intergenic
GAATACAAATGGAAAAGTAA+AGG 0.490240 2_2:+18927612 Msa0242610:CDS
GCAGGACTTAGTGTAGAATC+AGG 0.501466 2_2:+18928132 Msa0242610:CDS
GTCCACTTGTCTGTAACTTG+GGG 0.513310 2_2:+18928717 Msa0242610:three_prime_UTR
TCGATAAGTATTCATGTTCA+GGG 0.520119 2_2:-18928760 None:intergenic
TCTTGAAGGGTCATTGAGAA+TGG 0.527070 2_2:+18927149 Msa0242610:exon
TAGGATTTGTTTCGCAGTTG+CGG 0.527627 2_2:+18928095 Msa0242610:intron
GGATCCCAATTAATTAAAGA+AGG 0.531595 2_2:-18927769 None:intergenic
CATGTTAAGGATTTAGCAGA+AGG 0.532122 2_2:-18927799 None:intergenic
CCTTAATATAAAATCTCAAA+CGG 0.542784 2_2:+18926986 Msa0242610:five_prime_UTR
AGCCGAAGCCACACGTTCCG+AGG 0.544985 2_2:+18927293 Msa0242610:CDS
GCACAAGCAGCACATAACAA+AGG 0.553637 2_2:-18927676 None:intergenic
TAGGAATTGTCTCTCTTGAA+GGG 0.556722 2_2:+18927136 Msa0242610:intron
AACCCCAAGTTACAGACAAG+TGG 0.560699 2_2:-18928719 None:intergenic
AAGGTTTGTTCTCAGCTGCA+GGG 0.562389 2_2:+18928205 Msa0242610:CDS
GGCCCATTTGACCAAACCAA+AGG 0.574139 2_2:-18928516 None:intergenic
TATTCATGTTCAGGGACGAA+AGG 0.577708 2_2:-18928752 None:intergenic
AGGTTTGTTCTCAGCTGCAG+GGG 0.579049 2_2:+18928206 Msa0242610:CDS
GTTTGATATGATAGGTGACA+TGG 0.587795 2_2:+18927587 Msa0242610:intron
TACTCTACAAATTTATACAA+AGG 0.601260 2_2:-18928484 None:intergenic
CTTCTGCTAAATCCTTAACA+TGG 0.602252 2_2:+18927800 Msa0242610:CDS
AATTGCTACAGCATCAGAGA+AGG 0.604858 2_2:+18928186 Msa0242610:CDS
AAATTGTACCTCGGAACGTG+TGG 0.610751 2_2:-18927301 None:intergenic
TTAGGTATAAACCAGCAGCT+AGG 0.613595 2_2:-18928251 None:intergenic
CTTGTGAGTTTAGATCGGGT+TGG 0.639286 2_2:+18928695 Msa0242610:three_prime_UTR
TCGTGCACAAAGAATGTCTG+AGG 0.648802 2_2:+18928281 Msa0242610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAAAAATTGTACCT+CGG - chr2_2:18927313-18927332 None:intergenic 15.0%
!!! ATGTTAGTAATTAATTTGTT+AGG + chr2_2:18927353-18927372 Msa0242610:intron 15.0%
!!! TGTTAGTAATTAATTTGTTA+GGG + chr2_2:18927354-18927373 Msa0242610:intron 15.0%
!!! TGTTTTTTTGTAATAAATGA+AGG - chr2_2:18927887-18927906 None:intergenic 15.0%
!!! AGGTATAACTTTTATTAAAA+GGG - chr2_2:18928467-18928486 None:intergenic 15.0%
!!! AAGGTATAACTTTTATTAAA+AGG - chr2_2:18928468-18928487 None:intergenic 15.0%
!! TAATAAAAAAAATTGTACCT+CGG - chr2_2:18927313-18927332 None:intergenic 15.0%
!!! ATGTTAGTAATTAATTTGTT+AGG + chr2_2:18927353-18927372 Msa0242610:intron 15.0%
!!! TGTTAGTAATTAATTTGTTA+GGG + chr2_2:18927354-18927373 Msa0242610:intron 15.0%
!!! TGTTTTTTTGTAATAAATGA+AGG - chr2_2:18927887-18927906 None:intergenic 15.0%
!!! AGGTATAACTTTTATTAAAA+GGG - chr2_2:18928467-18928486 None:intergenic 15.0%
!!! AAGGTATAACTTTTATTAAA+AGG - chr2_2:18928468-18928487 None:intergenic 15.0%
!! CCTTAATATAAAATCTCAAA+CGG + chr2_2:18926986-18927005 Msa0242610:five_prime_UTR 20.0%
!!! AAATCTGTTTTACTTCTTTT+TGG - chr2_2:18927198-18927217 None:intergenic 20.0%
!!! GTTAGTAATTAATTTGTTAG+GGG + chr2_2:18927355-18927374 Msa0242610:intron 20.0%
!!! GTGTTAGTTTTAAAAAATCA+CGG - chr2_2:18927439-18927458 None:intergenic 20.0%
!! TCATGAAGTTTAATGTAATT+TGG + chr2_2:18927561-18927580 Msa0242610:intron 20.0%
!! AATACAAATGGAAAAGTAAA+GGG + chr2_2:18927613-18927632 Msa0242610:CDS 20.0%
!! AATGTGTATACAATAGTAAT+GGG + chr2_2:18927647-18927666 Msa0242610:CDS 20.0%
!! ATCACCTTCTTTAATTAATT+GGG + chr2_2:18927765-18927784 Msa0242610:CDS 20.0%
!!! AACACTTTTTTTTATGTTGT+AGG + chr2_2:18928076-18928095 Msa0242610:intron 20.0%
!! AATCAGGTCATTTAAAAAAT+TGG + chr2_2:18928148-18928167 Msa0242610:CDS 20.0%
!!! GGTATAACTTTTATTAAAAG+GGG - chr2_2:18928466-18928485 None:intergenic 20.0%
!! TACTCTACAAATTTATACAA+AGG - chr2_2:18928487-18928506 None:intergenic 20.0%
!! CCTTAATATAAAATCTCAAA+CGG + chr2_2:18926986-18927005 Msa0242610:five_prime_UTR 20.0%
!!! AAATCTGTTTTACTTCTTTT+TGG - chr2_2:18927198-18927217 None:intergenic 20.0%
!!! GTTAGTAATTAATTTGTTAG+GGG + chr2_2:18927355-18927374 Msa0242610:intron 20.0%
!!! GTGTTAGTTTTAAAAAATCA+CGG - chr2_2:18927439-18927458 None:intergenic 20.0%
!! TCATGAAGTTTAATGTAATT+TGG + chr2_2:18927561-18927580 Msa0242610:intron 20.0%
!! AATACAAATGGAAAAGTAAA+GGG + chr2_2:18927613-18927632 Msa0242610:CDS 20.0%
!! AATGTGTATACAATAGTAAT+GGG + chr2_2:18927647-18927666 Msa0242610:CDS 20.0%
!! ATCACCTTCTTTAATTAATT+GGG + chr2_2:18927765-18927784 Msa0242610:CDS 20.0%
!!! AACACTTTTTTTTATGTTGT+AGG + chr2_2:18928076-18928095 Msa0242610:intron 20.0%
!! AATCAGGTCATTTAAAAAAT+TGG + chr2_2:18928148-18928167 Msa0242610:CDS 20.0%
!!! GGTATAACTTTTATTAAAAG+GGG - chr2_2:18928466-18928485 None:intergenic 20.0%
!! TACTCTACAAATTTATACAA+AGG - chr2_2:18928487-18928506 None:intergenic 20.0%
!!! CCGTTTGAGATTTTATATTA+AGG - chr2_2:18926989-18927008 None:intergenic 25.0%
! AATATAAAATCTCAAACGGT+AGG + chr2_2:18926990-18927009 Msa0242610:intron 25.0%
!!! TTTTACTTCTTTTTGGTTCA+AGG - chr2_2:18927191-18927210 None:intergenic 25.0%
!! ATGGTTTTCACAATTCTATT+AGG + chr2_2:18927243-18927262 Msa0242610:CDS 25.0%
!! GTATTAAGAAAGTATGTTCA+AGG - chr2_2:18927412-18927431 None:intergenic 25.0%
!!! TTAGTTTTAAAAAATCACGG+AGG - chr2_2:18927436-18927455 None:intergenic 25.0%
! TGAAGTTTAATGTAATTTGG+TGG + chr2_2:18927564-18927583 Msa0242610:intron 25.0%
! GAAGTTTAATGTAATTTGGT+GGG + chr2_2:18927565-18927584 Msa0242610:intron 25.0%
! GAATACAAATGGAAAAGTAA+AGG + chr2_2:18927612-18927631 Msa0242610:CDS 25.0%
! GAATGTGTATACAATAGTAA+TGG + chr2_2:18927646-18927665 Msa0242610:CDS 25.0%
! CATCACCTTCTTTAATTAAT+TGG + chr2_2:18927764-18927783 Msa0242610:CDS 25.0%
!!! GTTTTTTCTTCATTACAACT+TGG + chr2_2:18927830-18927849 Msa0242610:CDS 25.0%
! TGTAATAAATGAAGGAACAA+AGG - chr2_2:18927879-18927898 None:intergenic 25.0%
!! TAACTTTTATTAAAAGGGGT+TGG - chr2_2:18928462-18928481 None:intergenic 25.0%
! AAAAACTTGTGAGTTTAGAT+CGG + chr2_2:18928690-18928709 Msa0242610:three_prime_UTR 25.0%
!!! CCGTTTGAGATTTTATATTA+AGG - chr2_2:18926989-18927008 None:intergenic 25.0%
! AATATAAAATCTCAAACGGT+AGG + chr2_2:18926990-18927009 Msa0242610:intron 25.0%
!!! TTTTACTTCTTTTTGGTTCA+AGG - chr2_2:18927191-18927210 None:intergenic 25.0%
!! ATGGTTTTCACAATTCTATT+AGG + chr2_2:18927243-18927262 Msa0242610:CDS 25.0%
!! GTATTAAGAAAGTATGTTCA+AGG - chr2_2:18927412-18927431 None:intergenic 25.0%
!!! TTAGTTTTAAAAAATCACGG+AGG - chr2_2:18927436-18927455 None:intergenic 25.0%
! TGAAGTTTAATGTAATTTGG+TGG + chr2_2:18927564-18927583 Msa0242610:intron 25.0%
! GAAGTTTAATGTAATTTGGT+GGG + chr2_2:18927565-18927584 Msa0242610:intron 25.0%
! GAATACAAATGGAAAAGTAA+AGG + chr2_2:18927612-18927631 Msa0242610:CDS 25.0%
! GAATGTGTATACAATAGTAA+TGG + chr2_2:18927646-18927665 Msa0242610:CDS 25.0%
! CATCACCTTCTTTAATTAAT+TGG + chr2_2:18927764-18927783 Msa0242610:CDS 25.0%
!!! GTTTTTTCTTCATTACAACT+TGG + chr2_2:18927830-18927849 Msa0242610:CDS 25.0%
! TGTAATAAATGAAGGAACAA+AGG - chr2_2:18927879-18927898 None:intergenic 25.0%
!! TAACTTTTATTAAAAGGGGT+TGG - chr2_2:18928462-18928481 None:intergenic 25.0%
! AAAAACTTGTGAGTTTAGAT+CGG + chr2_2:18928690-18928709 Msa0242610:three_prime_UTR 25.0%
!! AGTTCTATTCTAGAATTGCT+TGG - chr2_2:18927044-18927063 None:intergenic 30.0%
GGATCCCAATTAATTAAAGA+AGG - chr2_2:18927772-18927791 None:intergenic 30.0%
TAACACTAACATGTCGAAAT+TGG + chr2_2:18927938-18927957 Msa0242610:intron 30.0%
TTTGACTCTCAATTGTTGTA+GGG - chr2_2:18928413-18928432 None:intergenic 30.0%
!! TTTTGACTCTCAATTGTTGT+AGG - chr2_2:18928414-18928433 None:intergenic 30.0%
TAGAGTATAGTTTGATCCTT+TGG + chr2_2:18928500-18928519 Msa0242610:three_prime_UTR 30.0%
TATAGTTTGATCCTTTGGTT+TGG + chr2_2:18928505-18928524 Msa0242610:three_prime_UTR 30.0%
! TCAAATGGGCCAAATTTTTA+AGG + chr2_2:18928528-18928547 Msa0242610:three_prime_UTR 30.0%
TTCCACGTACCTTAAAAATT+TGG - chr2_2:18928540-18928559 None:intergenic 30.0%
AAAACTTGTGAGTTTAGATC+GGG + chr2_2:18928691-18928710 Msa0242610:three_prime_UTR 30.0%
!! AGTTCTATTCTAGAATTGCT+TGG - chr2_2:18927044-18927063 None:intergenic 30.0%
GGATCCCAATTAATTAAAGA+AGG - chr2_2:18927772-18927791 None:intergenic 30.0%
TAACACTAACATGTCGAAAT+TGG + chr2_2:18927938-18927957 Msa0242610:intron 30.0%
TTTGACTCTCAATTGTTGTA+GGG - chr2_2:18928413-18928432 None:intergenic 30.0%
!! TTTTGACTCTCAATTGTTGT+AGG - chr2_2:18928414-18928433 None:intergenic 30.0%
TAGAGTATAGTTTGATCCTT+TGG + chr2_2:18928500-18928519 Msa0242610:three_prime_UTR 30.0%
TATAGTTTGATCCTTTGGTT+TGG + chr2_2:18928505-18928524 Msa0242610:three_prime_UTR 30.0%
! TCAAATGGGCCAAATTTTTA+AGG + chr2_2:18928528-18928547 Msa0242610:three_prime_UTR 30.0%
TTCCACGTACCTTAAAAATT+TGG - chr2_2:18928540-18928559 None:intergenic 30.0%
AAAACTTGTGAGTTTAGATC+GGG + chr2_2:18928691-18928710 Msa0242610:three_prime_UTR 30.0%
TCAAGAGAGACAATTCCTAA+GGG - chr2_2:18927135-18927154 None:intergenic 35.0%
TTCAAGAGAGACAATTCCTA+AGG - chr2_2:18927136-18927155 None:intergenic 35.0%
TTAGGAATTGTCTCTCTTGA+AGG + chr2_2:18927135-18927154 Msa0242610:intron 35.0%
TAGGAATTGTCTCTCTTGAA+GGG + chr2_2:18927136-18927155 Msa0242610:intron 35.0%
ACAGATTTGAGTAGCAAAAC+AGG + chr2_2:18927210-18927229 Msa0242610:CDS 35.0%
! CAAAACAGGAGCTTTTCTTA+TGG + chr2_2:18927224-18927243 Msa0242610:CDS 35.0%
TTTAACGAATTGAAGCGACA+TGG - chr2_2:18927479-18927498 None:intergenic 35.0%
!! TTTGGTGGGTTTGATATGAT+AGG + chr2_2:18927579-18927598 Msa0242610:intron 35.0%
GTTTGATATGATAGGTGACA+TGG + chr2_2:18927587-18927606 Msa0242610:intron 35.0%
GTGACATGGATGAATACAAA+TGG + chr2_2:18927601-18927620 Msa0242610:CDS 35.0%
ACTAGCAATTGCTTTGGTTA+TGG + chr2_2:18927708-18927727 Msa0242610:CDS 35.0%
CATGTTAAGGATTTAGCAGA+AGG - chr2_2:18927802-18927821 None:intergenic 35.0%
CTTCTGCTAAATCCTTAACA+TGG + chr2_2:18927800-18927819 Msa0242610:CDS 35.0%
TGCTAAATCCTTAACATGGT+TGG + chr2_2:18927804-18927823 Msa0242610:CDS 35.0%
!! AGTTGCGGAGATTTTGTTAT+TGG + chr2_2:18928110-18928129 Msa0242610:CDS 35.0%
! ATGCTGTAGCAATTTGTTCT+TGG - chr2_2:18928180-18928199 None:intergenic 35.0%
! GCAATATGTCTTTGACTTTG+AGG - chr2_2:18928372-18928391 None:intergenic 35.0%
!! ATCCTTTGGTTTGGTCAAAT+GGG + chr2_2:18928514-18928533 Msa0242610:three_prime_UTR 35.0%
!!! TTTTTAAGGTACGTGGAAAG+TGG + chr2_2:18928542-18928561 Msa0242610:three_prime_UTR 35.0%
TCAAGAGAGACAATTCCTAA+GGG - chr2_2:18927135-18927154 None:intergenic 35.0%
TTCAAGAGAGACAATTCCTA+AGG - chr2_2:18927136-18927155 None:intergenic 35.0%
TTAGGAATTGTCTCTCTTGA+AGG + chr2_2:18927135-18927154 Msa0242610:intron 35.0%
TAGGAATTGTCTCTCTTGAA+GGG + chr2_2:18927136-18927155 Msa0242610:intron 35.0%
ACAGATTTGAGTAGCAAAAC+AGG + chr2_2:18927210-18927229 Msa0242610:CDS 35.0%
! CAAAACAGGAGCTTTTCTTA+TGG + chr2_2:18927224-18927243 Msa0242610:CDS 35.0%
TTTAACGAATTGAAGCGACA+TGG - chr2_2:18927479-18927498 None:intergenic 35.0%
!! TTTGGTGGGTTTGATATGAT+AGG + chr2_2:18927579-18927598 Msa0242610:intron 35.0%
GTTTGATATGATAGGTGACA+TGG + chr2_2:18927587-18927606 Msa0242610:intron 35.0%
GTGACATGGATGAATACAAA+TGG + chr2_2:18927601-18927620 Msa0242610:CDS 35.0%
ACTAGCAATTGCTTTGGTTA+TGG + chr2_2:18927708-18927727 Msa0242610:CDS 35.0%
CATGTTAAGGATTTAGCAGA+AGG - chr2_2:18927802-18927821 None:intergenic 35.0%
CTTCTGCTAAATCCTTAACA+TGG + chr2_2:18927800-18927819 Msa0242610:CDS 35.0%
TGCTAAATCCTTAACATGGT+TGG + chr2_2:18927804-18927823 Msa0242610:CDS 35.0%
!! AGTTGCGGAGATTTTGTTAT+TGG + chr2_2:18928110-18928129 Msa0242610:CDS 35.0%
! ATGCTGTAGCAATTTGTTCT+TGG - chr2_2:18928180-18928199 None:intergenic 35.0%
! GCAATATGTCTTTGACTTTG+AGG - chr2_2:18928372-18928391 None:intergenic 35.0%
!! ATCCTTTGGTTTGGTCAAAT+GGG + chr2_2:18928514-18928533 Msa0242610:three_prime_UTR 35.0%
!!! TTTTTAAGGTACGTGGAAAG+TGG + chr2_2:18928542-18928561 Msa0242610:three_prime_UTR 35.0%
TCTCAAACGGTAGGAATCTT+TGG + chr2_2:18926999-18927018 Msa0242610:intron 40.0%
TTGGAGCAAAGGTTGCTTAA+AGG - chr2_2:18927025-18927044 None:intergenic 40.0%
! TCTAGAATTGCTTGGAGCAA+AGG - chr2_2:18927036-18927055 None:intergenic 40.0%
! CTCTTCTTCTTTTCTCCCTT+AGG + chr2_2:18927117-18927136 Msa0242610:intron 40.0%
TCTTGAAGGGTCATTGAGAA+TGG + chr2_2:18927149-18927168 Msa0242610:exon 40.0%
TTGCATACCACCAAAAGAGT+AGG + chr2_2:18927522-18927541 Msa0242610:intron 40.0%
TGCATACCACCAAAAGAGTA+GGG + chr2_2:18927523-18927542 Msa0242610:intron 40.0%
! CATGTACTTCCCTACTCTTT+TGG - chr2_2:18927535-18927554 None:intergenic 40.0%
!! TGTTGGACTAGCAATTGCTT+TGG + chr2_2:18927702-18927721 Msa0242610:CDS 40.0%
GATTTAGCAGAAGGAGAATC+AGG - chr2_2:18927793-18927812 None:intergenic 40.0%
AAATCCTTAACATGGTTGGC+TGG + chr2_2:18927808-18927827 Msa0242610:CDS 40.0%
AAAACCAGCCAACCATGTTA+AGG - chr2_2:18927815-18927834 None:intergenic 40.0%
GTGTTAGTGACATGTTCAGA+TGG - chr2_2:18927924-18927943 None:intergenic 40.0%
! CATGTGTGTTGATATCGTGT+TGG + chr2_2:18927997-18928016 Msa0242610:intron 40.0%
! TAGGATTTGTTTCGCAGTTG+CGG + chr2_2:18928095-18928114 Msa0242610:intron 40.0%
AATTGCTACAGCATCAGAGA+AGG + chr2_2:18928186-18928205 Msa0242610:CDS 40.0%
TTAGGTATAAACCAGCAGCT+AGG - chr2_2:18928254-18928273 None:intergenic 40.0%
!! CTTTGACTTTGAGGTGATCT+TGG - chr2_2:18928363-18928382 None:intergenic 40.0%
!! GATCCTTTGGTTTGGTCAAA+TGG + chr2_2:18928513-18928532 Msa0242610:three_prime_UTR 40.0%
!!! GGCCAAATTTTTAAGGTACG+TGG + chr2_2:18928535-18928554 Msa0242610:three_prime_UTR 40.0%
TCTCAAACGGTAGGAATCTT+TGG + chr2_2:18926999-18927018 Msa0242610:intron 40.0%
TTGGAGCAAAGGTTGCTTAA+AGG - chr2_2:18927025-18927044 None:intergenic 40.0%
! TCTAGAATTGCTTGGAGCAA+AGG - chr2_2:18927036-18927055 None:intergenic 40.0%
! CTCTTCTTCTTTTCTCCCTT+AGG + chr2_2:18927117-18927136 Msa0242610:intron 40.0%
TCTTGAAGGGTCATTGAGAA+TGG + chr2_2:18927149-18927168 Msa0242610:exon 40.0%
TTGCATACCACCAAAAGAGT+AGG + chr2_2:18927522-18927541 Msa0242610:intron 40.0%
TGCATACCACCAAAAGAGTA+GGG + chr2_2:18927523-18927542 Msa0242610:intron 40.0%
! CATGTACTTCCCTACTCTTT+TGG - chr2_2:18927535-18927554 None:intergenic 40.0%
!! TGTTGGACTAGCAATTGCTT+TGG + chr2_2:18927702-18927721 Msa0242610:CDS 40.0%
GATTTAGCAGAAGGAGAATC+AGG - chr2_2:18927793-18927812 None:intergenic 40.0%
AAATCCTTAACATGGTTGGC+TGG + chr2_2:18927808-18927827 Msa0242610:CDS 40.0%
AAAACCAGCCAACCATGTTA+AGG - chr2_2:18927815-18927834 None:intergenic 40.0%
GTGTTAGTGACATGTTCAGA+TGG - chr2_2:18927924-18927943 None:intergenic 40.0%
! CATGTGTGTTGATATCGTGT+TGG + chr2_2:18927997-18928016 Msa0242610:intron 40.0%
! TAGGATTTGTTTCGCAGTTG+CGG + chr2_2:18928095-18928114 Msa0242610:intron 40.0%
AATTGCTACAGCATCAGAGA+AGG + chr2_2:18928186-18928205 Msa0242610:CDS 40.0%
TTAGGTATAAACCAGCAGCT+AGG - chr2_2:18928254-18928273 None:intergenic 40.0%
!! CTTTGACTTTGAGGTGATCT+TGG - chr2_2:18928363-18928382 None:intergenic 40.0%
!! GATCCTTTGGTTTGGTCAAA+TGG + chr2_2:18928513-18928532 Msa0242610:three_prime_UTR 40.0%
!!! GGCCAAATTTTTAAGGTACG+TGG + chr2_2:18928535-18928554 Msa0242610:three_prime_UTR 40.0%
AAATTGTACCTCGGAACGTG+TGG - chr2_2:18927304-18927323 None:intergenic 45.0%
! GTACTTCCCTACTCTTTTGG+TGG - chr2_2:18927532-18927551 None:intergenic 45.0%
GCACAAGCAGCACATAACAA+AGG - chr2_2:18927679-18927698 None:intergenic 45.0%
!!! TGTGCTGCTTGTGCTTTTGT+TGG + chr2_2:18927685-18927704 Msa0242610:CDS 45.0%
! GTGACATGTTCAGATGGACT+AGG - chr2_2:18927918-18927937 None:intergenic 45.0%
!!! GCGGAGATTTTGTTATTGGC+AGG + chr2_2:18928114-18928133 Msa0242610:CDS 45.0%
GCAGGACTTAGTGTAGAATC+AGG + chr2_2:18928132-18928151 Msa0242610:CDS 45.0%
! AAGGTTTGTTCTCAGCTGCA+GGG + chr2_2:18928205-18928224 Msa0242610:CDS 45.0%
! ACAACAGTTTTCCTAGCTGC+TGG + chr2_2:18928240-18928259 Msa0242610:CDS 45.0%
!! TTTGTGCACGAAGTGCTGTT+AGG - chr2_2:18928272-18928291 None:intergenic 45.0%
TCGTGCACAAAGAATGTCTG+AGG + chr2_2:18928281-18928300 Msa0242610:CDS 45.0%
!! TGACTTTGAGGTGATCTTGG+TGG - chr2_2:18928360-18928379 None:intergenic 45.0%
CTTGTGAGTTTAGATCGGGT+TGG + chr2_2:18928695-18928714 Msa0242610:three_prime_UTR 45.0%
TGGTCCACTTGTCTGTAACT+TGG + chr2_2:18928715-18928734 Msa0242610:three_prime_UTR 45.0%
GGTCCACTTGTCTGTAACTT+GGG + chr2_2:18928716-18928735 Msa0242610:three_prime_UTR 45.0%
GTCCACTTGTCTGTAACTTG+GGG + chr2_2:18928717-18928736 Msa0242610:three_prime_UTR 45.0%
AACCCCAAGTTACAGACAAG+TGG - chr2_2:18928722-18928741 None:intergenic 45.0%
AAATTGTACCTCGGAACGTG+TGG - chr2_2:18927304-18927323 None:intergenic 45.0%
! GTACTTCCCTACTCTTTTGG+TGG - chr2_2:18927532-18927551 None:intergenic 45.0%
GCACAAGCAGCACATAACAA+AGG - chr2_2:18927679-18927698 None:intergenic 45.0%
!!! TGTGCTGCTTGTGCTTTTGT+TGG + chr2_2:18927685-18927704 Msa0242610:CDS 45.0%
! GTGACATGTTCAGATGGACT+AGG - chr2_2:18927918-18927937 None:intergenic 45.0%
!!! GCGGAGATTTTGTTATTGGC+AGG + chr2_2:18928114-18928133 Msa0242610:CDS 45.0%
GCAGGACTTAGTGTAGAATC+AGG + chr2_2:18928132-18928151 Msa0242610:CDS 45.0%
! AAGGTTTGTTCTCAGCTGCA+GGG + chr2_2:18928205-18928224 Msa0242610:CDS 45.0%
! ACAACAGTTTTCCTAGCTGC+TGG + chr2_2:18928240-18928259 Msa0242610:CDS 45.0%
!! TTTGTGCACGAAGTGCTGTT+AGG - chr2_2:18928272-18928291 None:intergenic 45.0%
TCGTGCACAAAGAATGTCTG+AGG + chr2_2:18928281-18928300 Msa0242610:CDS 45.0%
!! TGACTTTGAGGTGATCTTGG+TGG - chr2_2:18928360-18928379 None:intergenic 45.0%
CTTGTGAGTTTAGATCGGGT+TGG + chr2_2:18928695-18928714 Msa0242610:three_prime_UTR 45.0%
TGGTCCACTTGTCTGTAACT+TGG + chr2_2:18928715-18928734 Msa0242610:three_prime_UTR 45.0%
GGTCCACTTGTCTGTAACTT+GGG + chr2_2:18928716-18928735 Msa0242610:three_prime_UTR 45.0%
GTCCACTTGTCTGTAACTTG+GGG + chr2_2:18928717-18928736 Msa0242610:three_prime_UTR 45.0%
AACCCCAAGTTACAGACAAG+TGG - chr2_2:18928722-18928741 None:intergenic 45.0%
! GCGACATGGACTGAACTTAG+TGG - chr2_2:18927465-18927484 None:intergenic 50.0%
! GAAGGTTTGTTCTCAGCTGC+AGG + chr2_2:18928204-18928223 Msa0242610:CDS 50.0%
! AGGTTTGTTCTCAGCTGCAG+GGG + chr2_2:18928206-18928225 Msa0242610:CDS 50.0%
GGCCCATTTGACCAAACCAA+AGG - chr2_2:18928519-18928538 None:intergenic 50.0%
! GCGACATGGACTGAACTTAG+TGG - chr2_2:18927465-18927484 None:intergenic 50.0%
! GAAGGTTTGTTCTCAGCTGC+AGG + chr2_2:18928204-18928223 Msa0242610:CDS 50.0%
! AGGTTTGTTCTCAGCTGCAG+GGG + chr2_2:18928206-18928225 Msa0242610:CDS 50.0%
GGCCCATTTGACCAAACCAA+AGG - chr2_2:18928519-18928538 None:intergenic 50.0%
TACCTCGGAACGTGTGGCTT+CGG - chr2_2:18927298-18927317 None:intergenic 55.0%
TACCTCGGAACGTGTGGCTT+CGG - chr2_2:18927298-18927317 None:intergenic 55.0%
AGCCGAAGCCACACGTTCCG+AGG + chr2_2:18927293-18927312 Msa0242610:CDS 65.0%
AGCCGAAGCCACACGTTCCG+AGG + chr2_2:18927293-18927312 Msa0242610:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 18926940 18928770 18926940 ID=Msa0242610;Name=Msa0242610
chr2_2 mRNA 18926940 18928770 18926940 ID=Msa0242610-mRNA-1;Parent=Msa0242610;Name=Msa0242610-mRNA-1;_AED=0.25;_eAED=0.25;_QI=97|1|1|1|0.66|0.5|4|276|264
chr2_2 exon 18926940 18927007 18926940 ID=Msa0242610-mRNA-1:exon:7733;Parent=Msa0242610-mRNA-1
chr2_2 exon 18927139 18927314 18927139 ID=Msa0242610-mRNA-1:exon:7734;Parent=Msa0242610-mRNA-1
chr2_2 exon 18927601 18927851 18927601 ID=Msa0242610-mRNA-1:exon:7735;Parent=Msa0242610-mRNA-1
chr2_2 exon 18928098 18928770 18928098 ID=Msa0242610-mRNA-1:exon:7736;Parent=Msa0242610-mRNA-1
chr2_2 five_prime_UTR 18926940 18927007 18926940 ID=Msa0242610-mRNA-1:five_prime_utr;Parent=Msa0242610-mRNA-1
chr2_2 five_prime_UTR 18927139 18927167 18927139 ID=Msa0242610-mRNA-1:five_prime_utr;Parent=Msa0242610-mRNA-1
chr2_2 CDS 18927168 18927314 18927168 ID=Msa0242610-mRNA-1:cds;Parent=Msa0242610-mRNA-1
chr2_2 CDS 18927601 18927851 18927601 ID=Msa0242610-mRNA-1:cds;Parent=Msa0242610-mRNA-1
chr2_2 CDS 18928098 18928494 18928098 ID=Msa0242610-mRNA-1:cds;Parent=Msa0242610-mRNA-1
chr2_2 three_prime_UTR 18928495 18928770 18928495 ID=Msa0242610-mRNA-1:three_prime_utr;Parent=Msa0242610-mRNA-1
Gene Sequence

>Msa0242610

ATGGCAGTTACACATGCAGACCTTGAACCAAAAAGAAGTAAAACAGATTTGAGTAGCAAAACAGGAGCTTTTCTTATGGTTTTCACAATTCTATTAGGCTTACTTTGTTTCATCTTATGTCTCATAGCCGAAGCCACACGTTCCGAGGTGACATGGATGAATACAAATGGAAAAGTAAAGGGAACTAAATCTGAATGTGTATACAATAGTAATGGGAAAGTGCCTTTGTTATGTGCTGCTTGTGCTTTTGTTGGACTAGCAATTGCTTTGGTTATGGAGCATACTTACATGTTGATAGCAGTGAGTAAATCATCACCTTCTTTAATTAATTGGGATCCTGATTCTCCTTCTGCTAAATCCTTAACATGGTTGGCTGGTTTTTTCTTCATTACAACTTGGATTTGTTTCGCAGTTGCGGAGATTTTGTTATTGGCAGGACTTAGTGTAGAATCAGGTCATTTAAAAAATTGGACTAAGCCAAGAACAAATTGCTACAGCATCAGAGAAGGTTTGTTCTCAGCTGCAGGGGTGTTTGCTTTAACAACAGTTTTCCTAGCTGCTGGTTTATACCTAACAGCACTTCGTGCACAAAGAATGTCTGAGGAGCTTGCAATTGTTAGAAGAGAAGTTCTTGAAGCTTCTAACTTTTATGCTTCTCCACCAAGATCACCTCAAAGTCAAAGACATATTGCAACTGTTGCAAGAGAGAACCCTACAACAATTGAGAGTCAAAATGATAATTTATTATTATCTGTGTTTCCAACCCCTTTTAATAAAAGTTATACCTTTGTATAA
Protein sequence

>Msa0242610

MAVTHADLEPKRSKTDLSSKTGAFLMVFTILLGLLCFILCLIAEATRSEVTWMNTNGKVKGTKSECVYNSNGKVPLLCAACAFVGLAIALVMEHTYMLIAVSKSSPSLINWDPDSPSAKSLTWLAGFFFITTWICFAVAEILLLAGLSVESGHLKNWTKPRTNCYSIREGLFSAAGVFALTTVFLAAGLYLTALRAQRMSEELAIVRREVLEASNFYASPPRSPQSQRHIATVARENPTTIESQNDNLLLSVFPTPFNKSYTFV*