Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0259410 | XP_024633040.1 | 97.368 | 456 | 3 | 2 | 1 | 456 | 1 | 447 | 0.0 | 904 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0259410 | sp|Q9LEU7|CIPK5_ARATH | 59.775 | 445 | 166 | 3 | 10 | 453 | 4 | 436 | 0.0 | 551 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0259410 | A0A072VCV6 | 97.345 | 452 | 3 | 2 | 1 | 452 | 1 | 443 | 0.0 | 894 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0259410 | Msa0259610 | 0.878868 | 1.981972e-69 | -8.615850e-47 |
| Msa0259410 | Msa0305590 | 0.922596 | 8.001632e-89 | -8.615850e-47 |
| Msa0259410 | Msa0624450 | 0.999254 | 8.806617e-299 | -8.615850e-47 |
| Msa0259410 | Msa0968020 | 0.948966 | 3.295638e-107 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0259410 | MtrunA17_Chr2g0333821 | 97.345 | 452 | 3 | 2 | 1 | 452 | 1 | 443 | 0.0 | 894 |
| Msa0259410 | MtrunA17_Chr4g0001331 | 74.353 | 464 | 97 | 4 | 1 | 455 | 1 | 451 | 0.0 | 706 |
| Msa0259410 | MtrunA17_Chr1g0150321 | 59.685 | 444 | 154 | 3 | 10 | 453 | 5 | 423 | 0.0 | 514 |
| Msa0259410 | MtrunA17_Chr4g0013131 | 51.902 | 447 | 199 | 4 | 9 | 453 | 3 | 435 | 9.34e-162 | 465 |
| Msa0259410 | MtrunA17_Chr7g0244521 | 51.242 | 443 | 205 | 5 | 10 | 450 | 4 | 437 | 7.06e-161 | 462 |
| Msa0259410 | MtrunA17_Chr5g0432471 | 49.890 | 453 | 214 | 6 | 13 | 453 | 7 | 458 | 9.89e-155 | 447 |
| Msa0259410 | MtrunA17_Chr2g0284031 | 51.111 | 450 | 198 | 7 | 12 | 447 | 25 | 466 | 3.31e-150 | 437 |
| Msa0259410 | MtrunA17_Chr3g0107011 | 48.770 | 447 | 217 | 5 | 6 | 450 | 2 | 438 | 2.49e-149 | 433 |
| Msa0259410 | MtrunA17_Chr8g0379091 | 48.498 | 466 | 216 | 8 | 2 | 447 | 8 | 469 | 7.43e-148 | 431 |
| Msa0259410 | MtrunA17_Chr2g0284011 | 48.115 | 451 | 220 | 4 | 10 | 456 | 4 | 444 | 2.65e-144 | 419 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0259410 | AT5G10930.1 | 59.775 | 445 | 166 | 3 | 10 | 453 | 4 | 436 | 0.0 | 551 |
| Msa0259410 | AT5G25110.1 | 58.462 | 455 | 173 | 3 | 1 | 455 | 29 | 467 | 0.0 | 549 |
| Msa0259410 | AT2G25090.1 | 56.018 | 457 | 179 | 6 | 6 | 453 | 3 | 446 | 4.93e-178 | 506 |
| Msa0259410 | AT5G45820.1 | 54.422 | 441 | 178 | 2 | 13 | 452 | 7 | 425 | 1.38e-169 | 484 |
| Msa0259410 | AT5G58380.1 | 51.339 | 448 | 213 | 3 | 10 | 453 | 4 | 450 | 9.90e-160 | 460 |
| Msa0259410 | AT4G18700.1 | 51.467 | 443 | 200 | 6 | 13 | 447 | 21 | 456 | 7.29e-154 | 446 |
| Msa0259410 | AT5G45810.1 | 50.110 | 453 | 213 | 8 | 2 | 446 | 13 | 460 | 6.71e-147 | 428 |
| Msa0259410 | AT5G01810.1 | 50.676 | 444 | 193 | 6 | 9 | 451 | 3 | 421 | 9.52e-146 | 422 |
| Msa0259410 | AT5G01810.2 | 50.676 | 444 | 193 | 6 | 9 | 451 | 3 | 421 | 9.52e-146 | 422 |
| Msa0259410 | AT4G30960.1 | 48.904 | 456 | 201 | 6 | 1 | 449 | 7 | 437 | 1.61e-144 | 420 |
Find 110 sgRNAs with CRISPR-Local
Find 250 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATCCTTGGTTTCAAAT+TGG | 0.197442 | 2_2:-56401494 | Msa0259410:CDS |
| AAAGCTGATATTTGGTCTTG+TGG | 0.567796 | 2_2:-56401704 | Msa0259410:CDS |
| AACGGGGAAGAAGGATTTCA+TGG | 0.504719 | 2_2:-56401150 | Msa0259410:CDS |
| AACTCCACCACTGCCACCTC+CGG | 0.439142 | 2_2:+56401056 | None:intergenic |
| AAGATCCTTGGTTTCAAATT+GGG | 0.349354 | 2_2:-56401493 | Msa0259410:CDS |
| AAGCTGAATTTGAGGGTAAC+GGG | 0.401989 | 2_2:-56401167 | Msa0259410:CDS |
| AATTATCTCAAACGCATTGT+AGG | 0.396765 | 2_2:+56401313 | None:intergenic |
| ACATAAGCCGGAGTCCCACA+CGG | 0.675390 | 2_2:+56401767 | None:intergenic |
| ACCAGAATGGATTTCACCGG+GGG | 0.612554 | 2_2:-56401585 | Msa0259410:CDS |
| ACGAGATAGGTAAAACTCTA+GGG | 0.561917 | 2_2:-56402243 | Msa0259410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCATCCAAAAGAAGATTCT+CGG | + | chr2_2:56401377-56401396 | None:intergenic | 30.0% | |
| GGATGAAAATGAAGATCTTA+AGG | - | chr2_2:56401390-56401409 | Msa0259410:CDS | 30.0% | |
| ATAACATTCTCACCTTGAAA+GGG | + | chr2_2:56401598-56401617 | None:intergenic | 30.0% | |
| CAAGGATCTTTCATAATATC+AGG | + | chr2_2:56401739-56401758 | None:intergenic | 30.0% | |
| CTGATATTATGAAAGATCCT+TGG | - | chr2_2:56401737-56401756 | Msa0259410:CDS | 30.0% | |
| AAAGATCCTTGGTTTCAAAT+TGG | - | chr2_2:56401748-56401767 | Msa0259410:CDS | 30.0% | |
| AAGATCCTTGGTTTCAAATT+GGG | - | chr2_2:56401749-56401768 | Msa0259410:CDS | 30.0% | |
| AATTATCTCAAACGCATTGT+AGG | + | chr2_2:56401932-56401951 | None:intergenic | 30.0% | |
| ATAATTTCATCGCTATCACA+TGG | - | chr2_2:56401946-56401965 | Msa0259410:CDS | 30.0% | |
| TATCACATGGATTTGATCTA+AGG | - | chr2_2:56401959-56401978 | Msa0259410:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 56400947 | 56402317 | 56400947 | ID=Msa0259410;Name=Msa0259410 |
| chr2_2 | mRNA | 56400947 | 56402317 | 56400947 | ID=Msa0259410-mRNA-1;Parent=Msa0259410;Name=Msa0259410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|456 |
| chr2_2 | exon | 56400947 | 56402317 | 56400947 | ID=Msa0259410-mRNA-1:exon:17078;Parent=Msa0259410-mRNA-1 |
| chr2_2 | CDS | 56400947 | 56402317 | 56400947 | ID=Msa0259410-mRNA-1:cds;Parent=Msa0259410-mRNA-1 |
| Gene Sequence |
>Msa0259410 ATGGAGCAACATAATCAAAATAATAGGAAAAGAAACATTCTGTTCAACAAATACGAGATAGGTAAAACTCTAGGGCAAGGAAATTTTGCAAAAGTGTATCATGGAAGAAACATAGCCACAAACGAAAACGTGGCTATCAAAGTGATTAAAAAAGAGAAGCTCAAAAAAGAGAGGCTGATGAAACAAATCAAACGTGAAGTTTCCGTGATGCGTCTCGTTCGTCATCCACATATCGTTGAGCTTAAGGAAGTCATGGCTAACAAGGCCAAAGTGTTCATGGTTGTTGAATACGTCAAAGGTGGTGAACTTTTCGCCAAAGTGGCCAAAGGTAAAATGGAAGAAAATATTGCCAGAAAGTATTTTCAACAATTGATCAGTGCTGTAGATTTCTGTCATAGTCGGGGAGTTACACACCGCGATCTTAAACCCGAGAATCTTCTTTTGGATGAAAATGAAGATCTTAAGGTGTCTGATTTTGGTTTATCAGCATTACCGGATCAACGAAGATCCGATGGAATGCTTCTCACACCGTGTGGGACTCCGGCTTATGTTGCACCGGAAGTTTTGAAGAAGATTGGTTATGATGGATCTAAAGCTGATATTTGGTCTTGTGGTGTTATTCTTTTTGCTTTGCTTTGTGGATATTTGCCCTTTCAAGGTGAGAATGTTATGAGGATTTATAGCAAATCTTTTAAAGCTGATTATGTTTTACCAGAATGGATTTCACCGGGGGCGAAGAAATTGATTTCTAATTTGCTTGTTGTTGATCCTGAAAAGAGGTTTTCAATTCCTGATATTATGAAAGATCCTTGGTTTCAAATTGGGTTTATGAGACCTATTGCTTTTTCAATGAAAGAATCTGCTGTCGATGATAACATTGATTTCAGTGGAGATGATGAAGGGAATGGCAACAATAGCAATGGTGATGGAAATTCTGTTGAGGTTGTGGGTGAAACTCGAACAAAACATGCTCGTAGACCATCCTACAATGCGTTTGAGATAATTTCATCGCTATCACATGGATTTGATCTAAGGAATTTGTTTGAGACGAGGAAGAGATCGCCTTCAATGTTTATATCGAAGCTGTCTGCTTCTGCGGTGGTGGGGAAGCTGGAGAATGTGGCGAAGAAGCTGAATTTGAGGGTAACGGGGAAGAAGGATTTCATGGTGAGAATGCAGGGGACAACAGAGGGGAGGAAGGGGAAATTGGGTATGACGGTGGAGGTGTTTGAGGTCGCACCGGAGGTGGCAGTGGTGGAGTTTTCTAAGTCAGCTGGAGACACACTTGAGTATGTAAAGTTCTGTGAAGATGAAGTTAGACCTTCTCTCAAAGACATTGTATGGAGCTGGCAGGGTGATACTCAATACTAA |
| Protein sequence |
>Msa0259410 MEQHNQNNRKRNILFNKYEIGKTLGQGNFAKVYHGRNIATNENVAIKVIKKEKLKKERLMKQIKREVSVMRLVRHPHIVELKEVMANKAKVFMVVEYVKGGELFAKVAKGKMEENIARKYFQQLISAVDFCHSRGVTHRDLKPENLLLDENEDLKVSDFGLSALPDQRRSDGMLLTPCGTPAYVAPEVLKKIGYDGSKADIWSCGVILFALLCGYLPFQGENVMRIYSKSFKADYVLPEWISPGAKKLISNLLVVDPEKRFSIPDIMKDPWFQIGFMRPIAFSMKESAVDDNIDFSGDDEGNGNNSNGDGNSVEVVGETRTKHARRPSYNAFEIISSLSHGFDLRNLFETRKRSPSMFISKLSASAVVGKLENVAKKLNLRVTGKKDFMVRMQGTTEGRKGKLGMTVEVFEVAPEVAVVEFSKSAGDTLEYVKFCEDEVRPSLKDIVWSWQGDTQY* |
