AlfalfaGEDB | Msa0260160

M. sativa cultivar zhongmu-4 / Msa0260160

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0260160	GAU44388.1	72.965	344	85	4	1	339	1	341	3.55e-125	372

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0260160	A0A2Z6NIP9	72.965	344	85	4	1	339	1	341	1.70e-125	372

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0260160	Msa0295590	0.815330	9.772250e-52	-8.615850e-47
Msa0260160	Msa1186130	0.800340	1.509125e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0260160	MtrunA17_Chr2g0317761	91.667	48	4	0	1	48	1	48	4.87e-22	95.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0260160	AT5G53800.1	65.868	167	56	1	174	339	185	351	1.82e-73	231

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Find 110 sgRNAs with CRISPR-Local

Find 368 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAAAATTTCACATAA+AGG	0.338744	2_2:+57685280	None:intergenic
AAAATGTCGAGAAACGTTAA+CGG	0.506060	2_2:-57690157	Msa0260160:CDS
AAAGGAAGGAGAAACGAAGG+AGG	0.491654	2_2:-57689796	Msa0260160:CDS
AACTCATTTCATGGATATTA+TGG	0.223435	2_2:-57685606	Msa0260160:three_prime_UTR
AAGACAAAACTTCCATCACA+AGG	0.671610	2_2:+57685467	None:intergenic
AAGAGAAAGAGAAGAAGAGA+AGG	0.469323	2_2:-57689742	Msa0260160:CDS
AAGGTGCTGTGACTAATTTA+TGG	0.342345	2_2:-57689640	Msa0260160:CDS
AATGAAGAAGAAGGAGAAAA+AGG	0.242745	2_2:-57689675	Msa0260160:CDS
AATTTCTGGTTACAGATTTA+TGG	0.131415	2_2:-57686722	Msa0260160:intron
ACAGTATGCACTATGTTCTG+AGG	0.640263	2_2:+57685214	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATGTCGAGAAACGTTAA+CGG	-	chr2_2:57685215-57685234	Msa0260160:three_prime_UTR	30.0%
AATGAAGAAGAAGGAGAAAA+AGG	-	chr2_2:57685697-57685716	Msa0260160:exon	30.0%
ACTAATTTATGGGGAAAGTA+TGG	-	chr2_2:57685743-57685762	Msa0260160:intron	30.0%
TTGATTGAAAGATGTCAGAT+TGG	-	chr2_2:57686026-57686045	Msa0260160:CDS	30.0%
GTTATTGTTATGCTTTGATC+CGG	-	chr2_2:57686071-57686090	Msa0260160:intron	30.0%
CTAACTCATGATTCAGTTTA+AGG	+	chr2_2:57686245-57686264	None:intergenic	30.0%
TATAAGTAATAACTTGGGAC+AGG	-	chr2_2:57686516-57686535	Msa0260160:intron	30.0%
TCAATATCCAATCACCTATT+TGG	+	chr2_2:57686565-57686584	None:intergenic	30.0%
GTGACATCCTAATAATATAG+AGG	-	chr2_2:57686586-57686605	Msa0260160:intron	30.0%
GACATCCTAATAATATAGAG+GGG	-	chr2_2:57686588-57686607	Msa0260160:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	57685202	57690192	57685202	ID=Msa0260160;Name=Msa0260160
chr2_2	mRNA	57685202	57690192	57685202	ID=Msa0260160-mRNA-1;Parent=Msa0260160;Name=Msa0260160-mRNA-1;_AED=0.10;_eAED=0.10;_QI=16\|0.71\|0.87\|0.87\|1\|1\|8\|497\|339
chr2_2	exon	57685202	57685746	57685202	ID=Msa0260160-mRNA-1:exon:17586;Parent=Msa0260160-mRNA-1
chr2_2	exon	57685834	57685905	57685834	ID=Msa0260160-mRNA-1:exon:17585;Parent=Msa0260160-mRNA-1
chr2_2	exon	57686009	57686087	57686009	ID=Msa0260160-mRNA-1:exon:17584;Parent=Msa0260160-mRNA-1
chr2_2	exon	57686403	57686516	57686403	ID=Msa0260160-mRNA-1:exon:17583;Parent=Msa0260160-mRNA-1
chr2_2	exon	57686688	57686729	57686688	ID=Msa0260160-mRNA-1:exon:17582;Parent=Msa0260160-mRNA-1
chr2_2	exon	57687023	57687072	57687023	ID=Msa0260160-mRNA-1:exon:17581;Parent=Msa0260160-mRNA-1
chr2_2	exon	57688246	57688288	57688246	ID=Msa0260160-mRNA-1:exon:17580;Parent=Msa0260160-mRNA-1
chr2_2	exon	57689605	57690192	57689605	ID=Msa0260160-mRNA-1:exon:17579;Parent=Msa0260160-mRNA-1
chr2_2	five_prime_UTR	57690177	57690192	57690177	ID=Msa0260160-mRNA-1:five_prime_utr;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57689605	57690176	57689605	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57688246	57688288	57688246	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57687023	57687072	57687023	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57686688	57686729	57686688	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57686403	57686516	57686403	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57686009	57686087	57686009	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57685834	57685905	57685834	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	CDS	57685699	57685746	57685699	ID=Msa0260160-mRNA-1:cds;Parent=Msa0260160-mRNA-1
chr2_2	three_prime_UTR	57685202	57685698	57685202	ID=Msa0260160-mRNA-1:three_prime_utr;Parent=Msa0260160-mRNA-1

Gene Sequence

>Msa0260160

ATGTCGAGAAACGTTAACGGTAATGGTGCTCCGGCGAAAATCGCCACCAGCTCAGAGAAAGACGACGGCGACGCTCCGTCGAAAAGAGATTCAAGGAAAACACGAGAATATTCCGATATAGACACACAAACCGATAGAAAGCATAGTAGCTCAAAGAAGAAATCGCGTGAGAGTTCAGATTCCGATTCCGATAAACGACGCCGTAGGAAGAGAAAATCACGTCGGAGATACAGCAGTGATGATGAATCGGATTCGGATTCCGGTTCGGATTCGGAGTCAGAGTGCGATGAATCCGAACGGTCTGGTTCGGAGTCGGAGTATTCGGGTTCGGAGAGTGAGGGAGAGAGGAAGGAGAGGAAAAGGAAGGAGAAACGAAGGAGGAGAGAGAGGGAGGAAGAGAAGAGAAGGAGGAAAGAGAAAGAGAAGAAGAGAAGGAAGAAGGAGAAAGAGGAAGAGAGAAGGAAGAAAGAGAAACGGAAAATGAAGAAGAAGGAGAAAAAGGAGAGAGGGAAGAAAGGTGCTGTGACTAATTTATGGGGAAAGTATGGGATTATTAGAGAAACTGATATGTGTCGTTATCCAGTGACTTACAGTTTAGCAAAATTTTATATCGAGGTAAATATGGAGCATCTGTCTAATTGGGAAGAAAAACAGATGTTCAAAGAATTTATGGAGGATCACAACACAGCTACGTTCCCTTCCAAAAAGTATTACAGTCTTGATGCTTACTACAGGCGTCAAATGGAAAAAGAGATGAAAAAGGGTTATAAGAAGGTTCAGCCTAAGGAACGAACAGTTTTCAATGACGAAGAACAGCGCAGACAAGAAATGTTGCAAGTACGTGAAAAACAGAAGGAAGAGCAAGTGATAGCTTTGAAGCGGGAAATGGAAAGTGGAATGGCACAAGCAATGAAAGAGCAAGCACAACTCAGGGAGGAGATGGCTTACCAGTACAAGCTTGGCAACTTTGAGGCTGCTGCTGCAATCCAGAGAAGGTTGGACCCTGATGCTGCCGTGTAG

Protein sequence

>Msa0260160

MSRNVNGNGAPAKIATSSEKDDGDAPSKRDSRKTREYSDIDTQTDRKHSSSKKKSRESSDSDSDKRRRRKRKSRRRYSSDDESDSDSGSDSESECDESERSGSESEYSGSESEGERKERKRKEKRRRREREEEKRRRKEKEKKRRKKEKEEERRKKEKRKMKKKEKKERGKKGAVTNLWGKYGIIRETDMCRYPVTYSLAKFYIEVNMEHLSNWEEKQMFKEFMEDHNTATFPSKKYYSLDAYYRRQMEKEMKKGYKKVQPKERTVFNDEEQRRQEMLQVREKQKEEQVIALKREMESGMAQAMKEQAQLREEMAYQYKLGNFEAAAAIQRRLDPDAAV*