Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0276910 | XP_003594187.1 | 98.578 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 6.27e-149 | 422 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0276910 | Q2HVF3 | 98.578 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 3.00e-149 | 422 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0276910 | Msa0316790 | 0.907648 | 4.038594e-81 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0276910 | MtrunA17_Chr2g0289601 | 98.578 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 5.77e-153 | 422 |
| Msa0276910 | MtrunA17_Chr4g0075561 | 65.138 | 109 | 28 | 3 | 1 | 106 | 1 | 102 | 1.24e-43 | 142 |
| Msa0276910 | MtrunA17_Chr6g0469421 | 41.096 | 219 | 83 | 7 | 1 | 211 | 1 | 181 | 5.80e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0276910 | AT1G12020.1 | 45.259 | 232 | 100 | 7 | 1 | 211 | 1 | 226 | 1.09e-49 | 161 |
| Msa0276910 | AT1G62422.1 | 46.119 | 219 | 79 | 7 | 1 | 211 | 1 | 188 | 3.47e-45 | 149 |
| Msa0276910 | AT5G13090.1 | 49.462 | 93 | 45 | 1 | 21 | 111 | 40 | 132 | 2.60e-22 | 92.0 |
| Msa0276910 | AT1G24270.1 | 47.872 | 94 | 40 | 2 | 23 | 116 | 86 | 170 | 3.65e-20 | 84.7 |
Find 63 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGATGAACCGTCGCTTTA+TGG | 0.220331 | 2_3:+9223081 | None:intergenic |
| AGAAAGGATTTGGGGTGTTT+GGG | 0.237919 | 2_3:-9222487 | Msa0276910:CDS |
| GAGAAAGGATTTGGGGTGTT+TGG | 0.279385 | 2_3:-9222488 | Msa0276910:CDS |
| AGAAAATTTGTGAGTTTCAT+TGG | 0.280803 | 2_3:-9222510 | Msa0276910:CDS |
| TCAGATGCTATACAAGTTAC+TGG | 0.294818 | 2_3:-9222845 | Msa0276910:CDS |
| GTTTCATTGGAGAAAGGATT+TGG | 0.305166 | 2_3:-9222497 | Msa0276910:CDS |
| TTTCATTGGAGAAAGGATTT+GGG | 0.320194 | 2_3:-9222496 | Msa0276910:CDS |
| AAGAATAAGAATAAGAATTC+TGG | 0.321116 | 2_3:-9222678 | Msa0276910:CDS |
| GTTGTTGCTGATGAAGAAAA+AGG | 0.371110 | 2_3:-9222540 | Msa0276910:CDS |
| GCTAGGACTTCTCTGTCTTC+AGG | 0.380809 | 2_3:+9222987 | None:intergenic |
| CGTGCTCCGTCGTGGGTTAC+CGG | 0.383486 | 2_3:+9222911 | None:intergenic |
| CAGGTGATAGCGCCGCCGCT+AGG | 0.396501 | 2_3:+9223006 | None:intergenic |
| TTTATGGTAGAGCTTCTTCA+TGG | 0.396994 | 2_3:+9223097 | None:intergenic |
| AGTGGTAAAGTTGGAAAGAA+AGG | 0.409989 | 2_3:-9222747 | Msa0276910:CDS |
| ATAGCGCCGCCGCTAGGGTT+AGG | 0.446267 | 2_3:+9223012 | None:intergenic |
| TTGTGAGTTTCATTGGAGAA+AGG | 0.453786 | 2_3:-9222503 | Msa0276910:CDS |
| AAAGAGAAGAGGAACAAGAA+AGG | 0.460478 | 2_3:-9222714 | Msa0276910:CDS |
| TAGGATGGTTGCGGGAAGGA+AGG | 0.465653 | 2_3:+9223031 | None:intergenic |
| GCTAGGGTTAGGATGGTTGC+GGG | 0.469356 | 2_3:+9223023 | None:intergenic |
| AGAATTCTGGTGAGTTGAAT+CGG | 0.470549 | 2_3:-9222665 | Msa0276910:CDS |
| AGAAGAGGAACAAGAAAGGA+GGG | 0.473041 | 2_3:-9222710 | Msa0276910:CDS |
| CGACGGAGCACGGTGAAGAC+AGG | 0.487092 | 2_3:-9222900 | Msa0276910:CDS |
| GAGAAATTGCTGAAAGTTGG+TGG | 0.490308 | 2_3:+9222933 | None:intergenic |
| CCGGAGAAATTGCTGAAAGT+TGG | 0.499193 | 2_3:+9222930 | None:intergenic |
| TTGGTTCAGAGTGGTAAAGT+TGG | 0.501650 | 2_3:-9222756 | Msa0276910:CDS |
| GTCTTCACCGTGCTCCGTCG+TGG | 0.502099 | 2_3:+9222903 | None:intergenic |
| CGCTAGGGTTAGGATGGTTG+CGG | 0.505574 | 2_3:+9223022 | None:intergenic |
| GTGGTAAAGTTGGAAAGAAA+GGG | 0.509636 | 2_3:-9222746 | Msa0276910:CDS |
| AGTAATAGTAGTAACAATAG+TGG | 0.510346 | 2_3:-9222594 | Msa0276910:CDS |
| TCATGTATGAGTTGACGATT+CGG | 0.513219 | 2_3:+9222804 | None:intergenic |
| GAGAAGAGGAACAAGAAAGG+AGG | 0.517489 | 2_3:-9222711 | Msa0276910:CDS |
| AACTACTGAGTTAGAATCTG+TGG | 0.517490 | 2_3:-9222625 | Msa0276910:CDS |
| TCGGTTATCACCGGTGGTGA+TGG | 0.517567 | 2_3:+9223062 | None:intergenic |
| CCAACTTTCAGCAATTTCTC+CGG | 0.520053 | 2_3:-9222930 | Msa0276910:CDS |
| GAAAGGATTTGGGGTGTTTG+GGG | 0.530002 | 2_3:-9222486 | Msa0276910:CDS |
| TCTTCACCGTGCTCCGTCGT+GGG | 0.530488 | 2_3:+9222904 | None:intergenic |
| AGGTGATAGCGCCGCCGCTA+GGG | 0.532801 | 2_3:+9223007 | None:intergenic |
| CAGATGCTATACAAGTTACT+GGG | 0.535574 | 2_3:-9222844 | Msa0276910:CDS |
| GGAAAGGAAAGAAAGAGAAG+AGG | 0.541545 | 2_3:-9222725 | Msa0276910:CDS |
| AATAGTAGTAACAATAGTGG+TGG | 0.552263 | 2_3:-9222591 | Msa0276910:CDS |
| AGAAATTGCTGAAAGTTGGT+GGG | 0.556471 | 2_3:+9222934 | None:intergenic |
| TTGCGGGAAGGAAGGAAAGT+TGG | 0.558904 | 2_3:+9223039 | None:intergenic |
| ATACAAGTTACTGGGTCAGA+TGG | 0.559401 | 2_3:-9222836 | Msa0276910:CDS |
| CGCCGCCGCTAGGGTTAGGA+TGG | 0.565059 | 2_3:+9223016 | None:intergenic |
| TTCATTGGAGAAAGGATTTG+GGG | 0.576638 | 2_3:-9222495 | Msa0276910:CDS |
| TGGAAGTGAAAATAGTGAAA+AGG | 0.578579 | 2_3:-9222571 | Msa0276910:CDS |
| CGGTAACCCACGACGGAGCA+CGG | 0.579056 | 2_3:-9222910 | Msa0276910:CDS |
| GAAAGTTGGTCGGTTATCAC+CGG | 0.579949 | 2_3:+9223053 | None:intergenic |
| GAGGAGTGGTTGGTTCAGAG+TGG | 0.580436 | 2_3:-9222765 | Msa0276910:CDS |
| AAAGTTGGAAAGAAAGGGAA+AGG | 0.584297 | 2_3:-9222741 | Msa0276910:CDS |
| TACAAGTTACTGGGTCAGAT+GGG | 0.590792 | 2_3:-9222835 | Msa0276910:CDS |
| GGGTTAGGATGGTTGCGGGA+AGG | 0.591167 | 2_3:+9223027 | None:intergenic |
| AACAATAGATGAGATAAGCT+AGG | 0.603361 | 2_3:+9222970 | None:intergenic |
| GAAAGTTGGTGGGGTTGTCG+TGG | 0.612483 | 2_3:+9222944 | None:intergenic |
| AACCATCCTAACCCTAGCGG+CGG | 0.614685 | 2_3:-9223018 | Msa0276910:CDS |
| GACGGTTCATCCATCACCAC+CGG | 0.616178 | 2_3:-9223072 | Msa0276910:CDS |
| ACTACTGAGTTAGAATCTGT+GGG | 0.616892 | 2_3:-9222624 | Msa0276910:CDS |
| AGTTGGTCGGTTATCACCGG+TGG | 0.641033 | 2_3:+9223056 | None:intergenic |
| GGGAAGGAAGGAAAGTTGGT+CGG | 0.644008 | 2_3:+9223043 | None:intergenic |
| GAAATTGCTGAAAGTTGGTG+GGG | 0.668665 | 2_3:+9222935 | None:intergenic |
| GAAGCTCTACCATAAAGCGA+CGG | 0.694050 | 2_3:-9223090 | Msa0276910:CDS |
| CGCAACCATCCTAACCCTAG+CGG | 0.725015 | 2_3:-9223021 | Msa0276910:CDS |
| ATTTCTCCGGTAACCCACGA+CGG | 0.736661 | 2_3:-9222917 | Msa0276910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAATAAGAATAAGAATTC+TGG | - | chr2_3:9222899-9222918 | Msa0276910:CDS | 20.0% |
| ! | AGTAATAGTAGTAACAATAG+TGG | - | chr2_3:9222983-9223002 | Msa0276910:CDS | 25.0% |
| ! | AGAAAATTTGTGAGTTTCAT+TGG | - | chr2_3:9223067-9223086 | Msa0276910:CDS | 25.0% |
| AACAATAGATGAGATAAGCT+AGG | + | chr2_3:9222610-9222629 | None:intergenic | 30.0% | |
| AATAGTAGTAACAATAGTGG+TGG | - | chr2_3:9222986-9223005 | Msa0276910:CDS | 30.0% | |
| TGGAAGTGAAAATAGTGAAA+AGG | - | chr2_3:9223006-9223025 | Msa0276910:CDS | 30.0% | |
| TTGTTGCTGATGAAGAAAAA+GGG | - | chr2_3:9223038-9223057 | Msa0276910:CDS | 30.0% | |
| TTTCATTGGAGAAAGGATTT+GGG | - | chr2_3:9223081-9223100 | Msa0276910:CDS | 30.0% | |
| ! | AGAAATTGCTGAAAGTTGGT+GGG | + | chr2_3:9222646-9222665 | None:intergenic | 35.0% |
| TCAGATGCTATACAAGTTAC+TGG | - | chr2_3:9222732-9222751 | Msa0276910:CDS | 35.0% | |
| CAGATGCTATACAAGTTACT+GGG | - | chr2_3:9222733-9222752 | Msa0276910:CDS | 35.0% | |
| ! | TCATGTATGAGTTGACGATT+CGG | + | chr2_3:9222776-9222795 | None:intergenic | 35.0% |
| AGTGGTAAAGTTGGAAAGAA+AGG | - | chr2_3:9222830-9222849 | Msa0276910:CDS | 35.0% | |
| GTGGTAAAGTTGGAAAGAAA+GGG | - | chr2_3:9222831-9222850 | Msa0276910:CDS | 35.0% | |
| AAAGTTGGAAAGAAAGGGAA+AGG | - | chr2_3:9222836-9222855 | Msa0276910:CDS | 35.0% | |
| AAAGAGAAGAGGAACAAGAA+AGG | - | chr2_3:9222863-9222882 | Msa0276910:CDS | 35.0% | |
| ! | AGAATTCTGGTGAGTTGAAT+CGG | - | chr2_3:9222912-9222931 | Msa0276910:CDS | 35.0% |
| AACTACTGAGTTAGAATCTG+TGG | - | chr2_3:9222952-9222971 | Msa0276910:CDS | 35.0% | |
| ACTACTGAGTTAGAATCTGT+GGG | - | chr2_3:9222953-9222972 | Msa0276910:CDS | 35.0% | |
| GTTGTTGCTGATGAAGAAAA+AGG | - | chr2_3:9223037-9223056 | Msa0276910:CDS | 35.0% | |
| TTGTGAGTTTCATTGGAGAA+AGG | - | chr2_3:9223074-9223093 | Msa0276910:CDS | 35.0% | |
| GTTTCATTGGAGAAAGGATT+TGG | - | chr2_3:9223080-9223099 | Msa0276910:CDS | 35.0% | |
| TTCATTGGAGAAAGGATTTG+GGG | - | chr2_3:9223082-9223101 | Msa0276910:CDS | 35.0% | |
| !! | GAAATTGCTGAAAGTTGGTG+GGG | + | chr2_3:9222645-9222664 | None:intergenic | 40.0% |
| GAGAAATTGCTGAAAGTTGG+TGG | + | chr2_3:9222647-9222666 | None:intergenic | 40.0% | |
| CCAACTTTCAGCAATTTCTC+CGG | - | chr2_3:9222647-9222666 | Msa0276910:CDS | 40.0% | |
| ATACAAGTTACTGGGTCAGA+TGG | - | chr2_3:9222741-9222760 | Msa0276910:CDS | 40.0% | |
| TACAAGTTACTGGGTCAGAT+GGG | - | chr2_3:9222742-9222761 | Msa0276910:CDS | 40.0% | |
| ! | TGAGATCATCGATGCTTTTG+AGG | - | chr2_3:9222793-9222812 | Msa0276910:CDS | 40.0% |
| TTGGTTCAGAGTGGTAAAGT+TGG | - | chr2_3:9222821-9222840 | Msa0276910:CDS | 40.0% | |
| GGAAAGGAAAGAAAGAGAAG+AGG | - | chr2_3:9222852-9222871 | Msa0276910:CDS | 40.0% | |
| AGAAGAGGAACAAGAAAGGA+GGG | - | chr2_3:9222867-9222886 | Msa0276910:CDS | 40.0% | |
| ! | AGAAAGGATTTGGGGTGTTT+GGG | - | chr2_3:9223090-9223109 | Msa0276910:CDS | 40.0% |
| GAAGCTCTACCATAAAGCGA+CGG | - | chr2_3:9222487-9222506 | Msa0276910:CDS | 45.0% | |
| ! | ATGGATGAACCGTCGCTTTA+TGG | + | chr2_3:9222499-9222518 | None:intergenic | 45.0% |
| ! | GAAAGTTGGTCGGTTATCAC+CGG | + | chr2_3:9222527-9222546 | None:intergenic | 45.0% |
| CCGGAGAAATTGCTGAAAGT+TGG | + | chr2_3:9222650-9222669 | None:intergenic | 45.0% | |
| !! | TCATCGATGCTTTTGAGGAG+TGG | - | chr2_3:9222798-9222817 | Msa0276910:CDS | 45.0% |
| GAGAAGAGGAACAAGAAAGG+AGG | - | chr2_3:9222866-9222885 | Msa0276910:CDS | 45.0% | |
| ! | GAGAAAGGATTTGGGGTGTT+TGG | - | chr2_3:9223089-9223108 | Msa0276910:CDS | 45.0% |
| ! | GAAAGGATTTGGGGTGTTTG+GGG | - | chr2_3:9223091-9223110 | Msa0276910:CDS | 45.0% |
| ! | GGGAAGGAAGGAAAGTTGGT+CGG | + | chr2_3:9222537-9222556 | None:intergenic | 50.0% |
| TTGCGGGAAGGAAGGAAAGT+TGG | + | chr2_3:9222541-9222560 | None:intergenic | 50.0% | |
| GCTAGGACTTCTCTGTCTTC+AGG | + | chr2_3:9222593-9222612 | None:intergenic | 50.0% | |
| ATTTCTCCGGTAACCCACGA+CGG | - | chr2_3:9222660-9222679 | Msa0276910:CDS | 50.0% | |
| !! | CGATGCTTTTGAGGAGTGGT+TGG | - | chr2_3:9222802-9222821 | Msa0276910:CDS | 50.0% |
| GACGGTTCATCCATCACCAC+CGG | - | chr2_3:9222505-9222524 | Msa0276910:CDS | 55.0% | |
| TCGGTTATCACCGGTGGTGA+TGG | + | chr2_3:9222518-9222537 | None:intergenic | 55.0% | |
| AGTTGGTCGGTTATCACCGG+TGG | + | chr2_3:9222524-9222543 | None:intergenic | 55.0% | |
| TAGGATGGTTGCGGGAAGGA+AGG | + | chr2_3:9222549-9222568 | None:intergenic | 55.0% | |
| GCTAGGGTTAGGATGGTTGC+GGG | + | chr2_3:9222557-9222576 | None:intergenic | 55.0% | |
| CGCTAGGGTTAGGATGGTTG+CGG | + | chr2_3:9222558-9222577 | None:intergenic | 55.0% | |
| CGCAACCATCCTAACCCTAG+CGG | - | chr2_3:9222556-9222575 | Msa0276910:CDS | 55.0% | |
| AACCATCCTAACCCTAGCGG+CGG | - | chr2_3:9222559-9222578 | Msa0276910:CDS | 55.0% | |
| !! | GAAAGTTGGTGGGGTTGTCG+TGG | + | chr2_3:9222636-9222655 | None:intergenic | 55.0% |
| ! | GAGGAGTGGTTGGTTCAGAG+TGG | - | chr2_3:9222812-9222831 | Msa0276910:CDS | 55.0% |
| GGGTTAGGATGGTTGCGGGA+AGG | + | chr2_3:9222553-9222572 | None:intergenic | 60.0% | |
| TCTTCACCGTGCTCCGTCGT+GGG | + | chr2_3:9222676-9222695 | None:intergenic | 60.0% | |
| ATAGCGCCGCCGCTAGGGTT+AGG | + | chr2_3:9222568-9222587 | None:intergenic | 65.0% | |
| ! | AGGTGATAGCGCCGCCGCTA+GGG | + | chr2_3:9222573-9222592 | None:intergenic | 65.0% |
| ! | CGTGCTCCGTCGTGGGTTAC+CGG | + | chr2_3:9222669-9222688 | None:intergenic | 65.0% |
| CGGTAACCCACGACGGAGCA+CGG | - | chr2_3:9222667-9222686 | Msa0276910:CDS | 65.0% | |
| GTCTTCACCGTGCTCCGTCG+TGG | + | chr2_3:9222677-9222696 | None:intergenic | 65.0% | |
| CGACGGAGCACGGTGAAGAC+AGG | - | chr2_3:9222677-9222696 | Msa0276910:CDS | 65.0% | |
| CGCCGCCGCTAGGGTTAGGA+TGG | + | chr2_3:9222564-9222583 | None:intergenic | 70.0% | |
| ! | CAGGTGATAGCGCCGCCGCT+AGG | + | chr2_3:9222574-9222593 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 9222482 | 9223117 | 9222482 | ID=Msa0276910;Name=Msa0276910 |
| chr2_3 | mRNA | 9222482 | 9223117 | 9222482 | ID=Msa0276910-mRNA-1;Parent=Msa0276910;Name=Msa0276910-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|211 |
| chr2_3 | exon | 9222482 | 9223117 | 9222482 | ID=Msa0276910-mRNA-1:exon:4231;Parent=Msa0276910-mRNA-1 |
| chr2_3 | CDS | 9222482 | 9223117 | 9222482 | ID=Msa0276910-mRNA-1:cds;Parent=Msa0276910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
