AlfalfaGEDB | Msa0301770

M. sativa cultivar zhongmu-4 / Msa0301770

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301770	RHN76022.1	91.489	329	28	0	19	347	75	403	0.0	624

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301770	sp\|Q9LQQ8\|PBL5_ARATH	67.901	324	99	4	19	340	67	387	2.29e-152	437

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301770	A0A396JFH9	91.489	329	28	0	19	347	75	403	0.0	624

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0222480	Msa0301770	0.831310	1.794889e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301770	MtrunA17_Chr2g0327541	91.489	329	28	0	19	347	75	403	0.0	624
Msa0301770	MtrunA17_Chr2g0327471	75.073	341	82	3	8	346	42	381	0.0	517
Msa0301770	MtrunA17_Chr2g0327511	74.041	339	85	3	11	347	62	399	2.78e-179	502
Msa0301770	MtrunA17_Chr4g0074651	69.767	344	101	3	6	347	72	414	1.37e-170	481
Msa0301770	MtrunA17_Chr2g0327531	67.925	318	99	3	31	346	17	333	6.83e-157	444
Msa0301770	MtrunA17_Chr7g0263521	64.856	313	106	4	32	342	49	359	1.58e-143	410
Msa0301770	MtrunA17_Chr1g0181821	64.610	308	106	3	42	347	59	365	1.41e-141	406
Msa0301770	MtrunA17_Chr2g0286281	61.981	313	115	4	32	342	65	375	5.90e-131	383
Msa0301770	MtrunA17_Chr1g0213991	62.000	300	111	3	42	339	82	380	5.25e-127	372
Msa0301770	MtrunA17_Chr7g0237401	56.863	306	127	4	41	342	77	381	2.74e-114	338

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0301770	AT1G07870.1	67.901	324	99	4	19	340	67	387	2.33e-153	437
Msa0301770	AT1G07870.2	68.111	323	98	4	20	340	68	387	5.15e-152	438
Msa0301770	AT2G28590.1	64.157	332	114	4	10	339	53	381	4.60e-147	422
Msa0301770	AT5G02800.1	63.844	307	108	3	42	346	58	363	5.73e-141	404
Msa0301770	AT5G18610.1	62.046	303	112	3	42	342	68	369	2.88e-130	382
Msa0301770	AT5G18610.2	62.046	303	112	3	42	342	68	369	2.88e-130	382
Msa0301770	AT5G18610.3	62.046	303	112	3	42	342	68	369	2.88e-130	382
Msa0301770	AT5G13160.1	54.348	368	137	7	3	340	4	370	4.25e-125	367
Msa0301770	AT2G28590.2	65.789	266	88	2	10	274	53	316	1.55e-118	345
Msa0301770	AT3G24790.1	57.475	301	125	3	37	335	27	326	1.39e-115	339

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Find 88 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACGGGGTTTCCACGGA+TGG	0.494546	2_3:+58287108	Msa0301770:CDS
AAGAATTTGAATTTGAAAGA+CGG	0.484333	2_3:+58287093	Msa0301770:CDS
AAGCCAAAATCAGTTAACTT+AGG	0.502997	2_3:-58288004	None:intergenic
AAGTCTAACATTTATAGCTT+CGG	0.419092	2_3:+58288130	Msa0301770:CDS
AATCGCCGGTCTCTGATTAA+CGG	0.518678	2_3:-58288856	None:intergenic
ACAAAATGGTGGAGCATTTG+AGG	0.466590	2_3:-58287964	None:intergenic
ACAAGACTGAGAATAGCTGT+TGG	0.672317	2_3:+58287881	Msa0301770:CDS
ACCTTAAAGGATCAATGGCG+TGG	0.631214	2_3:+58285971	None:intergenic
ACGAGCTTGCTGTTGCAACA+GGG	0.638611	2_3:+58287157	Msa0301770:CDS
ACTAGAATTTGTTCATCAGC+AGG	0.469499	2_3:-58288205	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAAAGGAAAAAAAAGGAC+TGG	-	chr2_3:58286124-58286143	None:intergenic	30.0%
CCAAAATGAAATTCTTAAGG+TGG	+	chr2_3:58286305-58286324	Msa0301770:intron	30.0%
AACAGTCATAAATCCTAAAC+AGG	-	chr2_3:58286515-58286534	None:intergenic	30.0%
GAGAAAGATAATCTACTATG+TGG	-	chr2_3:58286753-58286772	None:intergenic	30.0%
GTTCAATCAATACATCATAG+CGG	+	chr2_3:58286885-58286904	Msa0301770:intron	30.0%
TTCAATCAATACATCATAGC+GGG	+	chr2_3:58286886-58286905	Msa0301770:intron	30.0%
TAATCATGATCAACTTTCCT+TGG	+	chr2_3:58287065-58287084	Msa0301770:CDS	30.0%
ATTCAAATTCTTCACATCCA+AGG	-	chr2_3:58287085-58287104	None:intergenic	30.0%
GAATTTGAATTTGAAAGACG+GGG	+	chr2_3:58287095-58287114	Msa0301770:CDS	30.0%
CCAAATCTTGTCAAGTTAAT+TGG	+	chr2_3:58287483-58287502	Msa0301770:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	58285985	58289071	58285985	ID=Msa0301770;Name=Msa0301770
chr2_3	mRNA	58285985	58289071	58285985	ID=Msa0301770-mRNA-1;Parent=Msa0301770;Name=Msa0301770-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0\|0\|0\|0.8\|1\|1\|5\|0\|347
chr2_3	exon	58285985	58286000	58285985	ID=Msa0301770-mRNA-1:exon:18787;Parent=Msa0301770-mRNA-1
chr2_3	exon	58287031	58287257	58287031	ID=Msa0301770-mRNA-1:exon:18788;Parent=Msa0301770-mRNA-1
chr2_3	exon	58287393	58287582	58287393	ID=Msa0301770-mRNA-1:exon:18789;Parent=Msa0301770-mRNA-1
chr2_3	exon	58287846	58288234	58287846	ID=Msa0301770-mRNA-1:exon:18790;Parent=Msa0301770-mRNA-1
chr2_3	exon	58288850	58289071	58288850	ID=Msa0301770-mRNA-1:exon:18791;Parent=Msa0301770-mRNA-1
chr2_3	CDS	58285985	58286000	58285985	ID=Msa0301770-mRNA-1:cds;Parent=Msa0301770-mRNA-1
chr2_3	CDS	58287031	58287257	58287031	ID=Msa0301770-mRNA-1:cds;Parent=Msa0301770-mRNA-1
chr2_3	CDS	58287393	58287582	58287393	ID=Msa0301770-mRNA-1:cds;Parent=Msa0301770-mRNA-1
chr2_3	CDS	58287846	58288234	58287846	ID=Msa0301770-mRNA-1:cds;Parent=Msa0301770-mRNA-1
chr2_3	CDS	58288850	58289071	58288850	ID=Msa0301770-mRNA-1:cds;Parent=Msa0301770-mRNA-1

Gene Sequence

>Msa0301770

ATGGCGTGGTCTGCAGATGATGAAAGTGATGATGAAAGAGAAGACGATTCTAATCATGATCAACTTTCCTTGGATGTGAAGAATTTGAATTTGAAAGACGGGGTTTCCACGGATGGAAATGGTGCAAAGAAATTTACTTTTGACGAGCTTGCTGTTGCAACAGGGAATTTCAGGGCAGATTGCTTTGTTCGTGAAGTAGGGTTTGGAAAGGTTTATAAAGGTTACATCGAGAAAATAAATCAGGTTGTTGCAATAAAGCAACTTGACCCTATTGTGCTTCAAGGAAAAAGGAAATTTGTTTCTGAAGTGTCAAGACTCAGTTTAGCAGAACACCCAAATCTTGTCAAGTTAATTGGGTTTTGTGCTGAGGGAGAGCAAAGGCTATTGGTTTATGAGAACATGCCATTAGGATCATTAGAGAACCATTTGCATGATCTTTCCCCCGGGAAAATACCGCTGGACTGGAATACAAGACTGAGAATAGCTGTTGGTGTAGCTAGAGGTTTGGAGTACTTGCTTGATAAAATGAAGCCTCCCGTCCTATACCGTGACCTCAAATGCTCCACCATTTTGTTAGGCGAAGATTATCATCCTAAGTTAACTGATTTTGGCTTTGCAAAAGTAGACCCAATTGGTAATAAGATTTTTTCGTCAAGTGTTATGTGCTCATATGGGTATGGTGCTCTGGATTACGCAATGATGGGTCCATTGACATTCAAGTCTAACATTTATAGCTTCGGTGTTGTTCTTTTGGAGCTCATTACTGGCAGAAAGGCCATCGATTATAGCAAACCTGCTGATGAACAAATTCTAGTCGCATGGGCATGGCCGTTAATCAGAGACCGGCGATTCTCAGAGTTGGTGGATCCATTGCTTGAAGGTCGATATCCAGTGTGGGGTTTGTACCGGGCTCTCACTGTTGCTGCTATGTGTGTTCTGGAGGAGCAGCCTGATGTGCCACCCTCTATAGCCGATGTGGCCACAGTTCTAAATTACATTGCTACGCATCAGTACGATCCACAAGTTCATCCGATACAAAGCTAA

Protein sequence

>Msa0301770

MAWSADDESDDEREDDSNHDQLSLDVKNLNLKDGVSTDGNGAKKFTFDELAVATGNFRADCFVREVGFGKVYKGYIEKINQVVAIKQLDPIVLQGKRKFVSEVSRLSLAEHPNLVKLIGFCAEGEQRLLVYENMPLGSLENHLHDLSPGKIPLDWNTRLRIAVGVARGLEYLLDKMKPPVLYRDLKCSTILLGEDYHPKLTDFGFAKVDPIGNKIFSSSVMCSYGYGALDYAMMGPLTFKSNIYSFGVVLLELITGRKAIDYSKPADEQILVAWAWPLIRDRRFSELVDPLLEGRYPVWGLYRALTVAAMCVLEEQPDVPPSIADVATVLNYIATHQYDPQVHPIQS*