AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0309900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309900 MtrunA17_Chr2g0278541 95.567 406 5 2 1 405 1 394 0.0 778
Msa0309900 MtrunA17_Chr2g0278551 63.659 399 130 4 1 394 1 389 0.0 508
Msa0309900 MtrunA17_Chr2g0290611 64.658 365 115 3 8 372 8 358 8.68e-173 487
Msa0309900 MtrunA17_Chr2g0278561 59.494 395 145 5 6 397 9 391 2.80e-164 466
Msa0309900 MtrunA17_Chr4g0050941 61.053 380 147 1 1 379 1 380 9.51e-159 452
Msa0309900 MtrunA17_Chr4g0050891 63.526 329 118 1 4 332 5 331 3.48e-156 444
Msa0309900 MtrunA17_Chr4g0050881 57.925 347 141 2 9 355 6 347 5.72e-143 410
Msa0309900 MtrunA17_Chr4g0050931 58.310 355 109 1 1 355 1 316 5.28e-142 407
Msa0309900 MtrunA17_Chr2g0332001 49.223 386 181 5 1 385 27 398 1.19e-126 371
Msa0309900 MtrunA17_Chr4g0051021 49.153 354 174 3 6 358 8 356 1.54e-119 352
Msa0309900 MtrunA17_Chr4g0051001 48.553 311 156 1 5 315 24 330 3.87e-101 303
Msa0309900 MtrunA17_Chr2g0278021 54.682 267 116 2 126 389 1 265 1.43e-91 277
Msa0309900 MtrunA17_Chr2g0284261 38.150 346 201 3 12 354 8 343 6.59e-82 255
Msa0309900 MtrunA17_Chr7g0255561 38.485 330 196 4 12 339 14 338 6.89e-79 248
Msa0309900 MtrunA17_Chr7g0255581 38.485 330 196 4 12 339 14 338 6.89e-79 248
Msa0309900 MtrunA17_Chr1g0164831 37.572 346 198 5 12 354 3 333 9.93e-79 246
Msa0309900 MtrunA17_Chr8g0354121 36.074 377 229 7 6 376 7 377 1.41e-77 245
Msa0309900 MtrunA17_Chr1g0186611 38.286 350 203 5 11 358 15 353 2.23e-73 233
Msa0309900 MtrunA17_Chr3g0081421 37.994 329 198 3 12 338 17 341 1.72e-72 232
Msa0309900 MtrunA17_Chr1g0186601 37.322 351 209 5 7 356 11 351 1.75e-72 231
Msa0309900 MtrunA17_Chr7g0271561 35.792 366 233 1 7 370 2 367 3.29e-71 228
Msa0309900 MtrunA17_Chr3g0081511 38.602 329 191 5 12 337 17 337 8.90e-71 228
Msa0309900 MtrunA17_Chr1g0186641 37.278 338 200 4 2 337 3 330 1.59e-70 225
Msa0309900 MtrunA17_Chr7g0263101 32.821 390 233 6 12 395 13 379 3.05e-70 226
Msa0309900 MtrunA17_Chr8g0334761 34.648 355 213 5 13 360 11 353 2.77e-69 223
Msa0309900 MtrunA17_Chr3g0114731 34.974 386 239 6 12 395 18 393 1.11e-68 222
Msa0309900 MtrunA17_Chr1g0186571 37.389 337 201 4 2 337 3 330 1.30e-68 221
Msa0309900 MtrunA17_Chr1g0160171 36.747 332 203 2 6 337 10 334 2.14e-67 218
Msa0309900 MtrunA17_Chr1g0186561 39.589 341 192 6 2 337 3 334 2.21e-66 215
Msa0309900 MtrunA17_Chr1g0160111 35.519 366 224 4 21 383 16 372 1.31e-65 213
Msa0309900 MtrunA17_Chr3g0114691 35.429 350 215 4 11 357 19 360 7.27e-65 212
Msa0309900 MtrunA17_Chr3g0129571 36.364 330 203 5 11 337 6 331 3.06e-64 209
Msa0309900 MtrunA17_Chr6g0463971 35.362 345 212 5 12 355 16 350 6.10e-64 209
Msa0309900 MtrunA17_Chr1g0182291 33.714 350 222 3 6 354 9 349 9.76e-64 208
Msa0309900 MtrunA17_Chr1g0171301 36.585 328 200 3 11 337 15 335 6.60e-63 206
Msa0309900 MtrunA17_Chr3g0129591 35.714 364 208 7 11 371 4 344 2.71e-62 204
Msa0309900 MtrunA17_Chr1g0184221 32.337 368 216 4 5 354 9 361 3.30e-62 204
Msa0309900 MtrunA17_Chr2g0310441 33.234 334 211 3 13 343 11 335 1.12e-61 203
Msa0309900 MtrunA17_Chr5g0428881 32.546 381 241 4 12 391 18 383 1.77e-61 203
Msa0309900 MtrunA17_Chr4g0073241 31.892 370 235 7 7 371 3 360 8.52e-61 201
Msa0309900 MtrunA17_Chr8g0334771 34.024 338 213 3 13 348 11 340 7.48e-60 198
Msa0309900 MtrunA17_Chr3g0142131 36.705 346 213 3 17 356 25 370 1.10e-59 199
Msa0309900 MtrunA17_Chr3g0129601 35.714 350 213 5 8 355 25 364 2.47e-59 197
Msa0309900 MtrunA17_Chr6g0483401 35.000 320 203 2 21 339 19 334 2.81e-59 197
Msa0309900 MtrunA17_Chr5g0429001 34.627 335 205 4 62 392 1 325 2.85e-57 190
Msa0309900 MtrunA17_Chr5g0413961 33.429 347 219 4 6 352 8 342 4.91e-57 191
Msa0309900 MtrunA17_Chr5g0441761 32.416 327 209 2 17 343 19 333 7.71e-57 190
Msa0309900 MtrunA17_Chr3g0084091 35.333 300 184 2 93 392 11 300 1.74e-56 187
Msa0309900 MtrunA17_Chr8g0383731 36.970 330 199 4 12 337 5 329 1.11e-55 187
Msa0309900 MtrunA17_Chr1g0160181 36.364 330 202 4 12 339 18 341 1.13e-55 188
Msa0309900 MtrunA17_Chr8g0334751 32.295 353 228 4 1 351 1 344 1.71e-54 184
Msa0309900 MtrunA17_Chr1g0155761 35.097 359 201 6 17 353 26 374 1.84e-54 185
Msa0309900 MtrunA17_Chr3g0129581 34.060 367 210 7 11 371 4 344 1.87e-52 179
Msa0309900 MtrunA17_Chr3g0114711 29.676 401 236 6 3 365 5 397 3.91e-52 179
Msa0309900 MtrunA17_Chr3g0114701 32.031 384 243 7 3 383 2 370 4.47e-52 178
Msa0309900 MtrunA17_Chr3g0126101 33.724 341 210 5 13 351 7 333 6.88e-51 174
Msa0309900 MtrunA17_Chr5g0407971 32.162 370 217 6 17 372 16 365 2.70e-50 174
Msa0309900 MtrunA17_Chr4g0050921 73.077 104 28 0 215 318 1 104 4.74e-50 165
Msa0309900 MtrunA17_Chr2g0278031 60.800 125 49 0 1 125 3 127 5.64e-50 165
Msa0309900 MtrunA17_Chr4g0059311 31.707 328 212 5 13 337 12 330 9.10e-49 169
Msa0309900 MtrunA17_Chr1g0211801 34.615 260 150 4 12 265 11 256 3.36e-47 162
Msa0309900 MtrunA17_Chr5g0407991 33.432 338 216 3 15 344 12 348 4.50e-47 165
Msa0309900 MtrunA17_Chr5g0413941 29.787 329 220 5 17 343 12 331 4.08e-44 156
Msa0309900 MtrunA17_Chr5g0408031 33.144 353 205 7 7 339 5 346 1.06e-43 156
Msa0309900 MtrunA17_Chr5g0413921 29.518 332 223 4 8 337 3 325 1.77e-43 154
Msa0309900 MtrunA17_Chr8g0369841 30.168 358 224 6 17 357 14 362 4.47e-43 154
Msa0309900 MtrunA17_Chr1g0150151 31.307 329 214 5 13 337 12 332 8.62e-43 153
Msa0309900 MtrunA17_Chr4g0050911 57.252 131 54 1 87 215 6 136 3.71e-42 145
Msa0309900 MtrunA17_Chr5g0408041 31.638 354 211 7 11 344 10 352 9.02e-42 151
Msa0309900 MtrunA17_Chr6g0451171 30.973 339 220 6 1 337 1 327 1.34e-41 150
Msa0309900 MtrunA17_Chr5g0408001 31.933 357 219 6 17 357 15 363 1.41e-41 150
Msa0309900 MtrunA17_Chr7g0227921 29.282 362 235 6 11 370 10 352 4.51e-41 149
Msa0309900 MtrunA17_Chr6g0451211 29.589 365 235 9 1 357 1 351 2.14e-39 144
Msa0309900 MtrunA17_Chr5g0413931 29.118 340 226 6 16 350 10 339 3.94e-39 143
Msa0309900 MtrunA17_Chr5g0413951 30.154 325 212 6 17 337 12 325 4.75e-39 143
Msa0309900 MtrunA17_Chr6g0451251 30.194 361 237 7 1 357 1 350 2.99e-37 139
Msa0309900 MtrunA17_Chr6g0451201 30.423 355 231 6 1 354 1 340 3.37e-36 135
Msa0309900 MtrunA17_Chr6g0451261 29.450 309 209 4 11 318 10 310 6.64e-35 137
Msa0309900 MtrunA17_Chr5g0404811 32.283 254 165 4 120 371 1 249 8.43e-35 129
Msa0309900 MtrunA17_Chr1g0177221 32.031 256 163 4 12 265 18 264 1.13e-31 121
Msa0309900 MtrunA17_Chr3g0126091 26.727 333 222 8 13 336 12 331 2.38e-29 117
Msa0309900 MtrunA17_Chr8g0334711 35.047 214 126 5 1 212 1 203 3.22e-29 113
Msa0309900 MtrunA17_Chr6g0451191 31.050 219 125 4 127 337 2 202 2.45e-27 108
Msa0309900 MtrunA17_Chr8g0334721 34.694 147 94 2 229 374 1 146 3.91e-27 105
Msa0309900 MtrunA17_Chr5g0399281 29.831 295 174 9 57 337 53 328 3.15e-25 105
Msa0309900 MtrunA17_Chr4g0056081 27.879 330 212 9 20 337 22 337 1.61e-24 103
Msa0309900 MtrunA17_Chr4g0050901 67.692 65 21 0 1 65 1 65 3.28e-24 94.7
Msa0309900 MtrunA17_Chr8g0334731 32.353 204 106 8 13 212 12 187 1.88e-20 88.6
Msa0309900 MtrunA17_Chr1g0211811 37.363 91 53 1 267 357 1 87 8.36e-18 77.8
Msa0309900 MtrunA17_Chr1g0182261 34.646 127 76 3 219 340 89 213 1.32e-17 81.6
Msa0309900 MtrunA17_Chr1g0177231 40.000 95 53 1 266 360 29 119 1.43e-17 78.6
Msa0309900 MtrunA17_Chr1g0172891 38.947 95 51 1 276 370 17 104 1.52e-16 75.5
Msa0309900 MtrunA17_Chr5g0404801 33.962 106 70 0 17 122 12 117 7.18e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309900 AT1G25270.1 41.667 348 194 2 11 354 1 343 1.72e-94 288
Msa0309900 AT1G68170.1 43.605 344 190 1 11 354 1 340 2.34e-91 280
Msa0309900 AT1G68170.2 45.015 331 178 1 11 341 11 337 4.14e-91 279
Msa0309900 AT5G13670.1 39.946 373 214 5 12 383 7 370 7.11e-84 261
Msa0309900 AT3G30340.1 40.367 327 187 3 12 337 10 329 1.88e-77 244
Msa0309900 AT1G09380.1 40.683 322 188 1 13 334 8 326 3.19e-74 236
Msa0309900 AT5G07050.1 36.126 382 220 6 12 371 17 396 6.73e-72 231
Msa0309900 AT2G37460.1 37.082 329 197 3 12 339 14 333 2.62e-71 229
Msa0309900 AT4G01440.1 37.690 329 196 3 13 340 10 330 1.70e-70 226
Msa0309900 AT4G08290.1 33.085 402 245 3 1 402 1 378 2.58e-70 226
Msa0309900 AT1G01070.1 36.729 373 219 6 1 371 5 362 5.00e-67 217
Msa0309900 AT2G39510.1 35.215 372 220 7 12 380 7 360 9.13e-67 217
Msa0309900 AT1G21890.1 35.928 334 212 2 8 339 6 339 1.36e-65 214
Msa0309900 AT4G01430.1 38.623 334 190 6 13 340 9 333 3.30e-65 213
Msa0309900 AT3G56620.1 35.326 368 213 7 12 370 10 361 5.99e-64 209
Msa0309900 AT5G64700.1 38.253 332 197 4 12 339 7 334 2.51e-63 207
Msa0309900 AT4G08300.1 33.862 378 234 3 11 388 9 370 2.66e-63 208
Msa0309900 AT2G40900.1 35.604 323 196 2 12 334 10 320 2.70e-63 208
Msa0309900 AT4G01440.3 37.134 307 184 3 13 318 10 308 2.82e-63 206
Msa0309900 AT1G44800.1 35.457 361 220 5 1 359 1 350 4.70e-62 204
Msa0309900 AT4G30420.2 31.807 393 235 6 8 381 4 382 5.59e-62 204
Msa0309900 AT1G11450.3 36.592 358 215 6 1 356 5 352 3.69e-61 201
Msa0309900 AT3G18200.2 35.447 347 209 4 11 356 33 365 2.38e-60 200
Msa0309900 AT1G11460.1 36.667 330 197 6 13 339 16 336 6.12e-60 198
Msa0309900 AT4G30420.1 31.606 386 231 6 15 381 1 372 1.81e-59 198
Msa0309900 AT1G43650.1 36.857 350 208 4 12 361 6 342 5.67e-58 193
Msa0309900 AT1G75500.1 34.358 358 224 4 2 354 9 360 1.70e-57 193
Msa0309900 AT1G75500.2 34.358 358 224 4 2 354 9 360 1.70e-57 193
Msa0309900 AT4G01430.2 38.511 309 175 6 13 315 9 308 3.28e-56 187
Msa0309900 AT1G01070.2 36.050 319 188 5 55 371 11 315 7.28e-55 184
Msa0309900 AT1G21890.2 34.810 316 201 3 2 315 3 315 7.78e-55 185
Msa0309900 AT1G11450.5 37.061 313 186 5 46 356 5 308 1.11e-54 183
Msa0309900 AT3G53210.1 33.435 329 209 4 17 343 14 334 1.56e-53 182
Msa0309900 AT2G37450.2 31.921 354 201 5 13 356 9 332 3.88e-52 177
Msa0309900 AT1G11450.2 36.452 310 186 5 49 356 1 301 4.80e-52 176
Msa0309900 AT4G01450.2 34.513 339 209 3 13 350 10 336 2.48e-51 176
Msa0309900 AT3G30340.2 38.431 255 149 3 12 265 10 257 7.78e-51 172
Msa0309900 AT1G11450.4 36.593 317 189 6 1 315 5 311 3.82e-50 172
Msa0309900 AT4G28040.1 32.229 332 203 5 12 340 7 319 4.20e-49 170
Msa0309900 AT4G28040.5 32.229 332 203 5 12 340 7 319 4.20e-49 170
Msa0309900 AT4G28040.3 32.229 332 203 5 12 340 7 319 4.20e-49 170
Msa0309900 AT4G28040.4 32.229 332 203 5 12 340 7 319 4.20e-49 170
Msa0309900 AT4G28040.2 32.229 332 203 5 12 340 7 319 4.20e-49 170
Msa0309900 AT4G01450.1 33.660 306 190 3 13 317 10 303 1.16e-48 169
Msa0309900 AT4G01440.5 37.391 230 135 3 112 340 1 222 1.10e-47 164
Msa0309900 AT4G01440.2 37.391 230 135 3 112 340 1 222 1.10e-47 164
Msa0309900 AT3G18200.1 38.462 247 137 4 111 356 3 235 4.49e-46 159
Msa0309900 AT4G01440.4 35.827 254 154 3 13 265 10 255 1.97e-44 155
Msa0309900 AT5G45370.2 31.548 336 217 5 17 342 24 356 5.48e-44 157
Msa0309900 AT5G45370.4 31.548 336 217 5 17 342 24 356 5.48e-44 157
Msa0309900 AT5G45370.1 31.804 327 204 4 17 342 24 332 8.76e-44 156
Msa0309900 AT4G08290.2 33.585 265 171 2 1 265 1 260 1.88e-42 150
Msa0309900 AT5G40230.1 29.775 356 231 8 13 357 21 368 4.40e-42 152
Msa0309900 AT3G28050.1 29.014 355 232 7 13 364 12 349 4.63e-42 152
Msa0309900 AT2G37450.3 33.835 266 157 4 101 356 4 260 9.03e-42 148
Msa0309900 AT3G45870.1 33.437 323 204 5 17 332 16 334 1.21e-41 151
Msa0309900 AT3G53210.2 33.721 258 161 4 88 343 28 277 1.89e-41 149
Msa0309900 AT1G11450.1 35.821 268 161 5 91 356 2 260 3.28e-41 147
Msa0309900 AT5G64700.2 36.187 257 156 4 12 264 7 259 5.12e-41 146
Msa0309900 AT5G40210.1 33.028 327 188 8 17 339 17 316 3.35e-40 146
Msa0309900 AT5G40240.2 29.052 327 224 5 13 337 34 354 5.36e-40 147
Msa0309900 AT5G40240.1 29.052 327 224 5 13 337 20 340 5.37e-40 146
Msa0309900 AT5G40240.3 29.052 327 224 5 13 337 20 340 5.37e-40 146
Msa0309900 AT1G70260.1 27.222 360 236 7 11 359 10 354 2.47e-39 145
Msa0309900 AT4G19185.1 32.635 334 210 7 17 339 25 354 1.66e-38 143
Msa0309900 AT4G01450.4 32.271 251 157 3 13 262 10 248 6.30e-38 138
Msa0309900 AT3G45870.8 32.680 306 195 5 17 315 16 317 5.59e-36 134
Msa0309900 AT3G28100.1 28.614 339 225 9 17 349 18 345 7.12e-36 135
Msa0309900 AT4G01450.3 33.632 223 135 3 13 234 10 220 4.83e-35 130
Msa0309900 AT4G01430.3 38.049 205 113 5 141 340 1 196 6.25e-35 129
Msa0309900 AT4G19185.2 33.962 265 163 6 83 339 16 276 1.20e-34 130
Msa0309900 AT3G28070.1 27.635 351 226 10 17 351 21 359 2.92e-34 130
Msa0309900 AT4G15540.1 28.080 349 223 6 13 352 17 346 4.78e-34 130
Msa0309900 AT4G15540.2 28.080 349 223 6 13 352 17 346 4.78e-34 130
Msa0309900 AT3G28070.2 30.677 251 159 7 95 340 7 247 1.29e-33 127
Msa0309900 AT2G37450.1 34.239 184 111 2 183 356 110 293 4.19e-32 123
Msa0309900 AT3G28080.5 29.699 266 162 8 101 351 13 268 4.37e-32 122
Msa0309900 AT3G28080.7 29.699 266 162 8 101 351 13 268 4.37e-32 122
Msa0309900 AT3G28080.8 29.699 266 162 8 101 351 13 268 4.37e-32 122
Msa0309900 AT3G28080.6 29.699 266 162 8 101 351 13 268 4.37e-32 122
Msa0309900 AT5G45370.6 31.373 255 162 5 98 342 3 254 4.43e-32 123
Msa0309900 AT5G45370.5 31.373 255 162 5 98 342 3 254 4.43e-32 123
Msa0309900 AT3G28080.1 27.378 347 225 10 21 351 22 357 6.11e-32 124
Msa0309900 AT3G53210.3 30.677 251 164 4 17 265 14 256 1.65e-31 121
Msa0309900 AT3G28080.2 29.699 266 162 8 101 351 94 349 4.75e-31 122
Msa0309900 AT1G43650.2 35.200 250 152 2 15 264 9 248 4.95e-31 120
Msa0309900 AT5G47470.1 28.698 338 197 9 17 337 34 344 3.22e-30 120
Msa0309900 AT5G47470.3 28.698 338 197 9 17 337 62 372 3.36e-30 120
Msa0309900 AT1G60050.1 26.613 372 251 6 13 372 13 374 3.50e-30 120
Msa0309900 AT5G47470.2 28.698 338 197 9 17 337 62 372 3.53e-30 120
Msa0309900 AT4G24980.1 27.988 343 173 8 27 368 1 270 4.35e-30 117
Msa0309900 AT3G28130.2 26.911 327 226 7 17 339 17 334 3.89e-28 114
Msa0309900 AT3G28130.5 29.339 242 160 5 101 339 15 248 4.67e-28 112
Msa0309900 AT3G28130.1 29.339 242 160 5 101 339 15 248 4.67e-28 112
Msa0309900 AT3G45870.5 32.766 235 150 4 102 332 29 259 6.71e-27 109
Msa0309900 AT3G45870.9 32.812 256 161 5 17 265 16 267 2.38e-26 107
Msa0309900 AT4G16620.1 29.553 291 177 6 58 337 57 330 2.16e-25 106
Msa0309900 AT3G28070.3 26.144 306 207 9 17 315 21 314 5.35e-25 104
Msa0309900 AT3G28070.5 28.033 239 148 8 127 351 2 230 8.75e-25 102
Msa0309900 AT5G45370.3 30.493 223 142 5 127 339 74 293 2.23e-24 102
Msa0309900 AT3G28080.9 27.917 240 148 8 127 351 2 231 3.11e-24 100
Msa0309900 AT3G45870.6 31.651 218 141 4 102 315 29 242 1.38e-21 93.6
Msa0309900 AT3G45870.7 31.651 218 141 4 102 315 29 242 1.38e-21 93.6
Msa0309900 AT3G28130.3 26.389 216 148 5 127 339 2 209 2.57e-20 89.4
Msa0309900 AT3G45870.2 31.875 160 107 2 174 332 25 183 1.29e-18 84.7
Msa0309900 AT4G16620.4 28.340 247 150 5 101 337 4 233 3.28e-18 84.3
Msa0309900 AT4G16620.2 28.340 247 150 5 101 337 4 233 3.28e-18 84.3
Msa0309900 AT3G28100.3 26.587 252 169 8 17 262 18 259 1.87e-15 76.3
Msa0309900 AT4G16620.3 28.796 191 112 4 58 240 57 231 1.35e-13 70.9
Msa0309900 AT3G45870.3 31.548 168 107 4 102 265 29 192 2.17e-12 66.2
Msa0309900 AT3G28080.4 35.632 87 46 1 275 351 128 214 2.86e-12 66.2
Msa0309900 AT3G45870.4 32.414 145 93 3 125 265 2 145 3.16e-12 64.7
Msa0309900 AT3G28080.3 35.632 87 46 1 275 351 217 303 8.37e-12 66.2

Find 102 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTTATGGCTCATAATTC+AGG 0.235989 2_4:-4685544 Msa0309900:intron
CTGATGCCACGTGGCTATTT+TGG 0.237797 2_4:+4685631 None:intergenic
CTTGTATAGCTTCAGCTTCT+TGG 0.260057 2_4:+4684260 None:intergenic
GCAATACAAGCCACTGCTTT+TGG 0.264648 2_4:-4684998 Msa0309900:CDS
TCAAGAGCTATATATGAAAT+TGG 0.283248 2_4:+4687023 None:intergenic
GCATTTCTTTGTGGCTTGTT+TGG 0.289247 2_4:-4686594 Msa0309900:CDS
ATGGAGAAATTAATAGTATT+GGG 0.290488 2_4:-4684135 Msa0309900:CDS
ACTGCTTACCGTCTTGCTTT+TGG 0.295209 2_4:-4686837 Msa0309900:CDS
GTTCTCTATCTATATAATAT+TGG 0.297911 2_4:+4686993 None:intergenic
TTGTATAGCTTCAGCTTCTT+GGG 0.310222 2_4:+4684261 None:intergenic
GGAAGTTGGGTTGGAATATC+AGG 0.312561 2_4:-4684946 Msa0309900:CDS
CATTTCTTTGTGGCTTGTTT+GGG 0.333091 2_4:-4686593 Msa0309900:intron
AAAGTGTTGGGAACACTAAT+TGG 0.334567 2_4:-4685744 Msa0309900:CDS
GGTTGTTGCCTCTGGATTAG+TGG 0.357062 2_4:-4684799 Msa0309900:CDS
ATGCTGACTTTGGTAGTAAA+TGG 0.360193 2_4:-4685605 Msa0309900:CDS
TTATCATGATCCTCTACTAT+TGG 0.361628 2_4:+4684220 None:intergenic
AACAGTCATTCCATCATTGA+TGG 0.372529 2_4:+4686869 None:intergenic
AACTTCGTTGTGATAGTTGT+AGG 0.372704 2_4:+4684189 None:intergenic
GTCTTTGTGTTGAAAGAGAT+TGG 0.383963 2_4:-4684976 Msa0309900:CDS
GTGACAAACAATAGCATTAG+AGG 0.385978 2_4:+4684717 None:intergenic
TTCTTCTATGAAGGAATAAG+AGG 0.399482 2_4:-4686647 Msa0309900:intron
TAGTGGTTATTGTGACTTCA+TGG 0.399981 2_4:-4684782 Msa0309900:CDS
AATGGAGAAATTAATAGTAT+TGG 0.403749 2_4:-4684136 Msa0309900:CDS
GCATGAGATGGCCATGATAA+AGG 0.406811 2_4:+4686348 None:intergenic
AAGGTTTAGAAATTAATATC+TGG 0.429637 2_4:-4685689 Msa0309900:CDS
GATTGTGTGTGGGTTGTACA+TGG 0.434203 2_4:-4684344 Msa0309900:CDS
GCTGGAAAAGCCAAAGTGTT+GGG 0.436640 2_4:-4685756 Msa0309900:CDS
GGTGTTGATGTGGATGCATA+AGG 0.437855 2_4:+4685652 None:intergenic
TTGAAGACAGATGCAAATAA+AGG 0.440084 2_4:+4684741 None:intergenic
CCCTTGCTCTCATTTCTGAA+AGG 0.441072 2_4:-4686797 Msa0309900:intron
GTTTCGAAAGATTAAATTTG+AGG 0.444124 2_4:-4685785 Msa0309900:CDS
TTAAATTTGAGGGCTGCAGC+TGG 0.447893 2_4:-4685774 Msa0309900:CDS
TTTCGAAAGATTAAATTTGA+GGG 0.457389 2_4:-4685784 Msa0309900:CDS
TACAAACTTGCCATCAATGA+TGG 0.458995 2_4:-4686879 Msa0309900:CDS
AGTACCCTCCAAGTCAGCTT+TGG 0.459169 2_4:+4686624 None:intergenic
AAGCCAAAGCCTCATAGAAA+AGG 0.462142 2_4:+4686422 None:intergenic
ATAAGAGGCCAAAGCTGACT+TGG 0.467023 2_4:-4686632 Msa0309900:CDS
GACTTTGGTAGTAAATGGTT+GGG 0.467290 2_4:-4685600 Msa0309900:CDS
AGATTGGAGCCAATGGAAGT+TGG 0.470364 2_4:-4684960 Msa0309900:CDS
TCCAGCCATCACCTTTATCA+TGG 0.473066 2_4:-4686359 Msa0309900:CDS
GATTGGAGCCAATGGAAGTT+GGG 0.476744 2_4:-4684959 Msa0309900:CDS
TATTGTCGCATCTTTGATGT+TGG 0.486675 2_4:-4684694 Msa0309900:CDS
TTGGGAACACTAATTGGAAT+TGG 0.492275 2_4:-4685738 Msa0309900:CDS
TATTCCAACCCAACTTCCAT+TGG 0.494175 2_4:+4684951 None:intergenic
TTTGAAACCAGTGATGCTTA+TGG 0.499186 2_4:-4686940 Msa0309900:CDS
AATTAGTGTTCCCAACACTT+TGG 0.507004 2_4:+4685746 None:intergenic
GCCATGATAAAGGTGATGGC+TGG 0.507933 2_4:+4686358 None:intergenic
GCATCAGTCCATGCTGACTT+TGG 0.521527 2_4:-4685615 Msa0309900:CDS
TGGAGAAATTAATAGTATTG+GGG 0.524758 2_4:-4684134 Msa0309900:CDS
GCTACTAGCCGTGGCTTACT+CGG 0.525568 2_4:-4684924 Msa0309900:intron
ATATAGATAGAGAACAATGA+AGG 0.527032 2_4:-4686985 Msa0309900:exon
GGAACACTAATTGGAATTGG+TGG 0.527137 2_4:-4685735 Msa0309900:CDS
GGAGCCAATGGAAGTTGGGT+TGG 0.531439 2_4:-4684955 Msa0309900:CDS
ACCAGTGATGCTTATGGTGT+TGG 0.535166 2_4:-4686934 Msa0309900:CDS
AGCTGGAAAAGCCAAAGTGT+TGG 0.540163 2_4:-4685757 Msa0309900:CDS
ACCAACACCATAAGCATCAC+TGG 0.541033 2_4:+4686933 None:intergenic
GGGGTATGGAATGGAGTACA+TGG 0.541333 2_4:-4686963 Msa0309900:CDS
ACAGCTTTAATGTCTACAAT+GGG 0.541556 2_4:-4685022 Msa0309900:CDS
GTGGATGCATAAGGTTTATG+TGG 0.547363 2_4:+4685661 None:intergenic
TACAGCTTTAATGTCTACAA+TGG 0.548731 2_4:-4685023 Msa0309900:CDS
GAGAGCAAGGGGAACGGTGA+AGG 0.549091 2_4:+4686809 None:intergenic
GGAGCAGTGCTGATTGTGTG+TGG 0.553065 2_4:-4684355 Msa0309900:CDS
TGACTTTGGTAGTAAATGGT+TGG 0.557140 2_4:-4685601 Msa0309900:CDS
TATAGATAGAGAACAATGAA+GGG 0.557762 2_4:-4686984 Msa0309900:exon
ATGTAGTTCATTAGTTGACT+AGG 0.559962 2_4:+4683950 None:intergenic
GAACACTAATTGGAATTGGT+GGG 0.565650 2_4:-4685734 Msa0309900:CDS
GCTGTACTTGAGTAATGACT+TGG 0.568897 2_4:+4685040 None:intergenic
AATTAATTACCCGCATGAGA+TGG 0.570704 2_4:+4686336 None:intergenic
ACCTTTCAGAAATGAGAGCA+AGG 0.573982 2_4:+4686796 None:intergenic
AGAGGCCAAAGCTGACTTGG+AGG 0.577134 2_4:-4686629 Msa0309900:CDS
GAGCAGTGCTGATTGTGTGT+GGG 0.581248 2_4:-4684354 Msa0309900:CDS
TGCATAAGGTTTATGTGGAA+TGG 0.583430 2_4:+4685666 None:intergenic
TTCATGGTGCATAAAGATGA+GGG 0.589561 2_4:-4684766 Msa0309900:CDS
CCTTTCAGAAATGAGAGCAA+GGG 0.590764 2_4:+4686797 None:intergenic
TTGAAAGAGATTGGAGCCAA+TGG 0.591661 2_4:-4684967 Msa0309900:CDS
TTTATCATGGCCATCTCATG+CGG 0.601247 2_4:-4686346 Msa0309900:CDS
AGCAATATAGAGAAGTGTGA+AGG 0.602904 2_4:+4687047 None:intergenic
TTATCATGGCCATCTCATGC+GGG 0.603011 2_4:-4686345 Msa0309900:intron
GAGGCCAAAGCTGACTTGGA+GGG 0.606357 2_4:-4686628 Msa0309900:CDS
CACAATAACCACTAATCCAG+AGG 0.609597 2_4:+4684791 None:intergenic
ATGATGTTGATATTTCTCAA+AGG 0.609674 2_4:-4685708 Msa0309900:CDS
AACACTAATTGGAATTGGTG+GGG 0.614553 2_4:-4685733 Msa0309900:CDS
CTTTCAGAAATGAGAGCAAG+GGG 0.618664 2_4:+4686798 None:intergenic
AGAAATGAGAGCAAGGGGAA+CGG 0.619045 2_4:+4686803 None:intergenic
CTTCATGGTGCATAAAGATG+AGG 0.619627 2_4:-4684767 Msa0309900:CDS
GGAGAAATTAATAGTATTGG+GGG 0.623601 2_4:-4684133 Msa0309900:CDS
ATTTACTACCAAAGTCAGCA+TGG 0.624029 2_4:+4685607 None:intergenic
TAAAGGTGATGGCTGGAATG+AGG 0.627527 2_4:+4686365 None:intergenic
TGACAAACAATAGCATTAGA+GGG 0.632936 2_4:+4684718 None:intergenic
GAATATCAGGCTACTAGCCG+TGG 0.633998 2_4:-4684933 Msa0309900:CDS
TATATATACCGAGTAAGCCA+CGG 0.640373 2_4:+4684916 None:intergenic
AACACAAAGACCAAAAGCAG+TGG 0.644293 2_4:+4684988 None:intergenic
GTTGGGTGTTCTGTGTGCAG+TGG 0.650883 2_4:-4685583 Msa0309900:CDS
CATGGTGCATAAAGATGAGG+GGG 0.651969 2_4:-4684764 Msa0309900:CDS
ATAGATAGAGAACAATGAAG+GGG 0.658103 2_4:-4686983 Msa0309900:exon
TATGTCGACACCAATAGTAG+AGG 0.663735 2_4:-4684230 Msa0309900:CDS
TAGATAGAGAACAATGAAGG+GGG 0.664091 2_4:-4686982 Msa0309900:exon
GATGGCCATGATAAAGGTGA+TGG 0.667279 2_4:+4686354 None:intergenic
AGGCAGAGCCAAAAGCAAGA+CGG 0.673942 2_4:+4686829 None:intergenic
TCATGGTGCATAAAGATGAG+GGG 0.679612 2_4:-4684765 Msa0309900:CDS
CAGCATGGACTGATGCCACG+TGG 0.703750 2_4:+4685622 None:intergenic
TCAACACCAAAATAGCCACG+TGG 0.804683 2_4:-4685637 Msa0309900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTATGGTAATAT+AGG - chr2_4:4686268-4686287 Msa0309900:intron 10.0%
!!! ACATTTACATTTTTTTTAAA+AGG - chr2_4:4686442-4686461 Msa0309900:CDS 10.0%
!! AAAATGTAAAAGAAAAAAAA+AGG - chr2_4:4687034-4687053 Msa0309900:five_prime_UTR 10.0%
!! ATAATTTAGAAATTAAGAGA+AGG + chr2_4:4684155-4684174 None:intergenic 15.0%
!! TCTAAATTATAATTGCTTAT+AGG - chr2_4:4684165-4684184 Msa0309900:CDS 15.0%
!! TAGAGCAAATATTAATTAAA+TGG - chr2_4:4684223-4684242 Msa0309900:CDS 15.0%
!! ATATGAATAATAAATCAAAC+TGG - chr2_4:4684414-4684433 Msa0309900:intron 15.0%
!! ACTTTATTATTACATTACAT+AGG - chr2_4:4684631-4684650 Msa0309900:intron 15.0%
!! TTAATATTTGGAAAATTCTA+TGG - chr2_4:4684655-4684674 Msa0309900:intron 15.0%
!!! AGTTTCAAGTAAAATATTAA+GGG + chr2_4:4685090-4685109 None:intergenic 15.0%
!!! AAGTTTCAAGTAAAATATTA+AGG + chr2_4:4685091-4685110 None:intergenic 15.0%
!!! TTTTCTTCACAAAATAAAAA+GGG + chr2_4:4685412-4685431 None:intergenic 15.0%
!!! TTTTTCTTCACAAAATAAAA+AGG + chr2_4:4685413-4685432 None:intergenic 15.0%
!!! ATGTTTGATTTTAATATTGT+AGG - chr2_4:4686083-4686102 Msa0309900:intron 15.0%
!!! TGTTTGATTTTAATATTGTA+GGG - chr2_4:4686084-4686103 Msa0309900:intron 15.0%
!! TTGGATGAAAAATTATATTT+AGG - chr2_4:4686231-4686250 Msa0309900:intron 15.0%
!! ATGAAAAATTATATTTAGGA+AGG - chr2_4:4686235-4686254 Msa0309900:intron 15.0%
!!! TTACATTTTTTTTAAAAGGA+TGG - chr2_4:4686446-4686465 Msa0309900:intron 15.0%
!! GTTCTCTATCTATATAATAT+TGG + chr2_4:4683916-4683935 None:intergenic 20.0%
!! TATTTGCTCTATATATAGTT+AGG + chr2_4:4684214-4684233 None:intergenic 20.0%
!! AGATATTAATTGCATGAAAA+TGG + chr2_4:4684604-4684623 None:intergenic 20.0%
!! CATTACATAGGTTTAATATT+TGG - chr2_4:4684643-4684662 Msa0309900:intron 20.0%
!! TTTCGAAAGATTAAATTTGA+GGG - chr2_4:4685122-4685141 Msa0309900:intron 20.0%
!! AAGGTTTAGAAATTAATATC+TGG - chr2_4:4685217-4685236 Msa0309900:intron 20.0%
!! GATCATAAGAAAAATAACTT+AGG + chr2_4:4685511-4685530 None:intergenic 20.0%
!!! AAAGATGTTTATGTTTTCAA+AGG + chr2_4:4685615-4685634 None:intergenic 20.0%
!!! GTTGTTTAATTGACATTTTT+TGG - chr2_4:4685810-4685829 Msa0309900:intron 20.0%
!!! GTTTGATTTTAATATTGTAG+GGG - chr2_4:4686085-4686104 Msa0309900:intron 20.0%
!!! TTTGATTTTAATATTGTAGG+GGG - chr2_4:4686086-4686105 Msa0309900:intron 20.0%
!! TAACATTCTAACTAATATGA+TGG - chr2_4:4686488-4686507 Msa0309900:intron 20.0%
!!! GTAGATAATTTGTCAAAAAA+TGG - chr2_4:4686752-4686771 Msa0309900:intron 20.0%
!! AATGGAGAAATTAATAGTAT+TGG - chr2_4:4686770-4686789 Msa0309900:intron 20.0%
!! ATGGAGAAATTAATAGTATT+GGG - chr2_4:4686771-4686790 Msa0309900:intron 20.0%
!!! TTGTATTTTTTCTTCTTCTA+AGG - chr2_4:4686999-4687018 Msa0309900:five_prime_UTR 20.0%
!!! TGTATTTTTTCTTCTTCTAA+GGG - chr2_4:4687000-4687019 Msa0309900:five_prime_UTR 20.0%
! TCAAGAGCTATATATGAAAT+TGG + chr2_4:4683886-4683905 None:intergenic 25.0%
! ATATAGATAGAGAACAATGA+AGG - chr2_4:4683921-4683940 Msa0309900:three_prime_UTR 25.0%
! TATAGATAGAGAACAATGAA+GGG - chr2_4:4683922-4683941 Msa0309900:three_prime_UTR 25.0%
!!! TGTTTTGTTTTCTTCTATGA+AGG - chr2_4:4684250-4684269 Msa0309900:CDS 25.0%
!! ATTTCAAAACCTTTTCTATG+AGG - chr2_4:4684475-4684494 Msa0309900:intron 25.0%
! GTTTCGAAAGATTAAATTTG+AGG - chr2_4:4685121-4685140 Msa0309900:intron 25.0%
! ATGATGTTGATATTTCTCAA+AGG - chr2_4:4685198-4685217 Msa0309900:intron 25.0%
! TTCTTATGATCCATAACAAA+CGG - chr2_4:4685520-4685539 Msa0309900:intron 25.0%
!! AAATAAAGATCCGTTTGTTA+TGG + chr2_4:4685533-4685552 None:intergenic 25.0%
! AGAATAAAGAGCAAATAAGA+GGG + chr2_4:4685573-4685592 None:intergenic 25.0%
! TAGAATAAAGAGCAAATAAG+AGG + chr2_4:4685574-4685593 None:intergenic 25.0%
! AAATAAGATAGTTGCTCTTT+AGG + chr2_4:4685678-4685697 None:intergenic 25.0%
! TGGAGAAATTAATAGTATTG+GGG - chr2_4:4686772-4686791 Msa0309900:intron 25.0%
!!! AATATTTTATTGAAGTGCTC+CGG - chr2_4:4686835-4686854 Msa0309900:CDS 25.0%
ATAGATAGAGAACAATGAAG+GGG - chr2_4:4683923-4683942 Msa0309900:three_prime_UTR 30.0%
TTCTTCTATGAAGGAATAAG+AGG - chr2_4:4684259-4684278 Msa0309900:CDS 30.0%
ATAACCATATACGCTAGTAA+TGG - chr2_4:4684348-4684367 Msa0309900:CDS 30.0%
AGTTATGTAATGAATGTTGC+AGG - chr2_4:4684444-4684463 Msa0309900:intron 30.0%
GTTATGTAATGAATGTTGCA+GGG - chr2_4:4684445-4684464 Msa0309900:intron 30.0%
TTATGTAATGAATGTTGCAG+GGG - chr2_4:4684446-4684465 Msa0309900:intron 30.0%
!!! AGTCATGAGAATGACTTTTT+TGG - chr2_4:4684681-4684700 Msa0309900:CDS 30.0%
ATGTCATTGTACAAATAGTG+GGG + chr2_4:4684747-4684766 None:intergenic 30.0%
GATGTCATTGTACAAATAGT+GGG + chr2_4:4684748-4684767 None:intergenic 30.0%
TGATGTCATTGTACAAATAG+TGG + chr2_4:4684749-4684768 None:intergenic 30.0%
GTACAATGACATCAAAAGTT+CGG - chr2_4:4684755-4684774 Msa0309900:CDS 30.0%
! GATGAATTGATGATACTATG+AGG + chr2_4:4684782-4684801 None:intergenic 30.0%
TCTTAAGTGTTACACTTTGT+GGG + chr2_4:4684862-4684881 None:intergenic 30.0%
AAATATTAAGGGAGCTTCTA+CGG + chr2_4:4685079-4685098 None:intergenic 30.0%
! GATGTTTATGTTTTCAAAGG+TGG + chr2_4:4685612-4685631 None:intergenic 30.0%
!!! AATTGACATTTTTTGGCTCT+TGG - chr2_4:4685817-4685836 Msa0309900:intron 30.0%
! TACAGCTTTAATGTCTACAA+TGG - chr2_4:4685883-4685902 Msa0309900:intron 30.0%
! ACAGCTTTAATGTCTACAAT+GGG - chr2_4:4685884-4685903 Msa0309900:intron 30.0%
TTGAAGACAGATGCAAATAA+AGG + chr2_4:4686168-4686187 None:intergenic 30.0%
TGACAAACAATAGCATTAGA+GGG + chr2_4:4686191-4686210 None:intergenic 30.0%
!! GTTTTGTTGTATAGTGCAAT+TGG - chr2_4:4686530-4686549 Msa0309900:intron 30.0%
TTATCATGATCCTCTACTAT+TGG + chr2_4:4686689-4686708 None:intergenic 30.0%
! GGAGAAATTAATAGTATTGG+GGG - chr2_4:4686773-4686792 Msa0309900:intron 30.0%
ATGTAGTTCATTAGTTGACT+AGG + chr2_4:4686959-4686978 None:intergenic 30.0%
!! TTTTCTTCTTCTAAGGGTAA+TGG - chr2_4:4687006-4687025 Msa0309900:five_prime_UTR 30.0%
TAGATAGAGAACAATGAAGG+GGG - chr2_4:4683924-4683943 Msa0309900:three_prime_UTR 35.0%
! TTTGAAACCAGTGATGCTTA+TGG - chr2_4:4683966-4683985 Msa0309900:three_prime_UTR 35.0%
TACAAACTTGCCATCAATGA+TGG - chr2_4:4684027-4684046 Msa0309900:three_prime_UTR 35.0%
AACAGTCATTCCATCATTGA+TGG + chr2_4:4684040-4684059 None:intergenic 35.0%
! CTTTTCATGGCATTTCTTTG+TGG - chr2_4:4684303-4684322 Msa0309900:CDS 35.0%
!! CATTTCTTTGTGGCTTGTTT+GGG - chr2_4:4684313-4684332 Msa0309900:CDS 35.0%
TTGACCATTACTAGCGTATA+TGG + chr2_4:4684355-4684374 None:intergenic 35.0%
! AAACCTTTTCTATGAGGCTT+TGG - chr2_4:4684481-4684500 Msa0309900:intron 35.0%
AATTAATTACCCGCATGAGA+TGG + chr2_4:4684573-4684592 None:intergenic 35.0%
CTTAAGTGTTACACTTTGTG+GGG + chr2_4:4684861-4684880 None:intergenic 35.0%
GTCTTAAGTGTTACACTTTG+TGG + chr2_4:4684863-4684882 None:intergenic 35.0%
TAGTAATGCTTGCAACATCT+TGG + chr2_4:4684953-4684972 None:intergenic 35.0%
AATTAGTGTTCCCAACACTT+TGG + chr2_4:4685163-4685182 None:intergenic 35.0%
!! AAAGTGTTGGGAACACTAAT+TGG - chr2_4:4685162-4685181 Msa0309900:intron 35.0%
TTGGGAACACTAATTGGAAT+TGG - chr2_4:4685168-4685187 Msa0309900:intron 35.0%
GAACACTAATTGGAATTGGT+GGG - chr2_4:4685172-4685191 Msa0309900:intron 35.0%
AACACTAATTGGAATTGGTG+GGG - chr2_4:4685173-4685192 Msa0309900:intron 35.0%
TGCATAAGGTTTATGTGGAA+TGG + chr2_4:4685243-4685262 None:intergenic 35.0%
ATTTACTACCAAAGTCAGCA+TGG + chr2_4:4685302-4685321 None:intergenic 35.0%
!! ATGCTGACTTTGGTAGTAAA+TGG - chr2_4:4685301-4685320 Msa0309900:intron 35.0%
! TGACTTTGGTAGTAAATGGT+TGG - chr2_4:4685305-4685324 Msa0309900:intron 35.0%
! GACTTTGGTAGTAAATGGTT+GGG - chr2_4:4685306-4685325 Msa0309900:intron 35.0%
! GTTGCTTCTCTTTTGCTTTA+TGG - chr2_4:4685349-4685368 Msa0309900:intron 35.0%
TGCTTTATGGCTCATAATTC+AGG - chr2_4:4685362-4685381 Msa0309900:intron 35.0%
AAGATCTGTTTGCTCATTTG+TGG - chr2_4:4685648-4685667 Msa0309900:CDS 35.0%
AGATCTGTTTGCTCATTTGT+GGG - chr2_4:4685649-4685668 Msa0309900:CDS 35.0%
AAGCTACGCATGCTTATAAA+AGG + chr2_4:4685733-4685752 None:intergenic 35.0%
GTCTTTGTGTTGAAAGAGAT+TGG - chr2_4:4685930-4685949 Msa0309900:intron 35.0%
TATATATACCGAGTAAGCCA+CGG + chr2_4:4685993-4686012 None:intergenic 35.0%
TAGTGGTTATTGTGACTTCA+TGG - chr2_4:4686124-4686143 Msa0309900:intron 35.0%
TTCATGGTGCATAAAGATGA+GGG - chr2_4:4686140-4686159 Msa0309900:intron 35.0%
GTGACAAACAATAGCATTAG+AGG + chr2_4:4686192-4686211 None:intergenic 35.0%
TATTGTCGCATCTTTGATGT+TGG - chr2_4:4686212-4686231 Msa0309900:intron 35.0%
AAATTGAATGCGTGACTTCT+CGG - chr2_4:4686355-4686374 Msa0309900:CDS 35.0%
GATGAAAAAGATTGCTCAGT+TGG - chr2_4:4686607-4686626 Msa0309900:CDS 35.0%
TTGTATAGCTTCAGCTTCTT+GGG + chr2_4:4686648-4686667 None:intergenic 35.0%
AACTTCGTTGTGATAGTTGT+AGG + chr2_4:4686720-4686739 None:intergenic 35.0%
CTTTCAGAAATGAGAGCAAG+GGG + chr2_4:4684111-4684130 None:intergenic 40.0%
CCTTTCAGAAATGAGAGCAA+GGG + chr2_4:4684112-4684131 None:intergenic 40.0%
ACCTTTCAGAAATGAGAGCA+AGG + chr2_4:4684113-4684132 None:intergenic 40.0%
!! GCATTTCTTTGTGGCTTGTT+TGG - chr2_4:4684312-4684331 Msa0309900:CDS 40.0%
AAGCCAAAGCCTCATAGAAA+AGG + chr2_4:4684487-4684506 None:intergenic 40.0%
TTTATCATGGCCATCTCATG+CGG - chr2_4:4684560-4684579 Msa0309900:intron 40.0%
! GGGGTGTTACACTTATAACA+AGG + chr2_4:4684842-4684861 None:intergenic 40.0%
TTGCAACATCTTGGACTTAC+AGG + chr2_4:4684944-4684963 None:intergenic 40.0%
GGAACACTAATTGGAATTGG+TGG - chr2_4:4685171-4685190 Msa0309900:intron 40.0%
GTGGATGCATAAGGTTTATG+TGG + chr2_4:4685248-4685267 None:intergenic 40.0%
!!! TGGCTATTTTGGTGTTGATG+TGG + chr2_4:4685267-4685286 None:intergenic 40.0%
ATAAGCATGCGTAGCTTCTT+TGG - chr2_4:4685736-4685755 Msa0309900:CDS 40.0%
!!! CATTTTTTGGCTCTTGGCAT+AGG - chr2_4:4685823-4685842 Msa0309900:intron 40.0%
GCTGTACTTGAGTAATGACT+TGG + chr2_4:4685869-4685888 None:intergenic 40.0%
AACACAAAGACCAAAAGCAG+TGG + chr2_4:4685921-4685940 None:intergenic 40.0%
TTGAAAGAGATTGGAGCCAA+TGG - chr2_4:4685939-4685958 Msa0309900:intron 40.0%
TATTCCAACCCAACTTCCAT+TGG + chr2_4:4685958-4685977 None:intergenic 40.0%
CACAATAACCACTAATCCAG+AGG + chr2_4:4686118-4686137 None:intergenic 40.0%
CTTCATGGTGCATAAAGATG+AGG - chr2_4:4686139-4686158 Msa0309900:intron 40.0%
TCATGGTGCATAAAGATGAG+GGG - chr2_4:4686141-4686160 Msa0309900:intron 40.0%
AATTGAATGCGTGACTTCTC+GGG - chr2_4:4686356-4686375 Msa0309900:CDS 40.0%
!!! TGGGTTGTACATGGTTTTGT+GGG - chr2_4:4686571-4686590 Msa0309900:intron 40.0%
!!! GCTTCTTGGGTGTTTTTTGA+TGG + chr2_4:4686635-4686654 None:intergenic 40.0%
CTTGTATAGCTTCAGCTTCT+TGG + chr2_4:4686649-4686668 None:intergenic 40.0%
TATGTCGACACCAATAGTAG+AGG - chr2_4:4686676-4686695 Msa0309900:intron 40.0%
AGAGAACAATGAAGGGGGTA+TGG - chr2_4:4683929-4683948 Msa0309900:three_prime_UTR 45.0%
! ACAATGAAGGGGGTATGGAA+TGG - chr2_4:4683934-4683953 Msa0309900:three_prime_UTR 45.0%
ACCAGTGATGCTTATGGTGT+TGG - chr2_4:4683972-4683991 Msa0309900:three_prime_UTR 45.0%
ACCAACACCATAAGCATCAC+TGG + chr2_4:4683976-4683995 None:intergenic 45.0%
! ACTGCTTACCGTCTTGCTTT+TGG - chr2_4:4684069-4684088 Msa0309900:three_prime_UTR 45.0%
AGAAATGAGAGCAAGGGGAA+CGG + chr2_4:4684106-4684125 None:intergenic 45.0%
CCCTTGCTCTCATTTCTGAA+AGG - chr2_4:4684109-4684128 Msa0309900:exon 45.0%
ATAAGAGGCCAAAGCTGACT+TGG - chr2_4:4684274-4684293 Msa0309900:CDS 45.0%
!!! GACTTGGAGGGTACTTTTCA+TGG - chr2_4:4684290-4684309 Msa0309900:CDS 45.0%
! TAAAGGTGATGGCTGGAATG+AGG + chr2_4:4684544-4684563 None:intergenic 45.0%
TCCAGCCATCACCTTTATCA+TGG - chr2_4:4684547-4684566 Msa0309900:intron 45.0%
GATGGCCATGATAAAGGTGA+TGG + chr2_4:4684555-4684574 None:intergenic 45.0%
GCATGAGATGGCCATGATAA+AGG + chr2_4:4684561-4684580 None:intergenic 45.0%
TTATCATGGCCATCTCATGC+GGG - chr2_4:4684561-4684580 Msa0309900:intron 45.0%
TTAAATTTGAGGGCTGCAGC+TGG - chr2_4:4685132-4685151 Msa0309900:intron 45.0%
AGCTGGAAAAGCCAAAGTGT+TGG - chr2_4:4685149-4685168 Msa0309900:intron 45.0%
! GCTGGAAAAGCCAAAGTGTT+GGG - chr2_4:4685150-4685169 Msa0309900:intron 45.0%
!! GGTGTTGATGTGGATGCATA+AGG + chr2_4:4685257-4685276 None:intergenic 45.0%
TCAACACCAAAATAGCCACG+TGG - chr2_4:4685269-4685288 Msa0309900:intron 45.0%
! GCAATACAAGCCACTGCTTT+TGG - chr2_4:4685908-4685927 Msa0309900:intron 45.0%
AGATTGGAGCCAATGGAAGT+TGG - chr2_4:4685946-4685965 Msa0309900:intron 45.0%
GATTGGAGCCAATGGAAGTT+GGG - chr2_4:4685947-4685966 Msa0309900:intron 45.0%
!! GGAAGTTGGGTTGGAATATC+AGG - chr2_4:4685960-4685979 Msa0309900:intron 45.0%
CATGGTGCATAAAGATGAGG+GGG - chr2_4:4686142-4686161 Msa0309900:intron 45.0%
! GATTGTGTGTGGGTTGTACA+TGG - chr2_4:4686562-4686581 Msa0309900:intron 45.0%
!! GTGGGTTGTACATGGTTTTG+TGG - chr2_4:4686570-4686589 Msa0309900:intron 45.0%
!!! GGGTTGTACATGGTTTTGTG+GGG - chr2_4:4686572-4686591 Msa0309900:intron 45.0%
! CTCAGCAACTTCTTTTCGTC+CGG + chr2_4:4686857-4686876 None:intergenic 45.0%
!! TAATGTAATAAATATATATA+AGG - chr2_4:4684383-4684402 Msa0309900:intron 5.0%
!!! AAATTTGTTTTTTTTTTTTA+TGG - chr2_4:4686259-4686278 Msa0309900:intron 5.0%
GGGGTATGGAATGGAGTACA+TGG - chr2_4:4683943-4683962 Msa0309900:three_prime_UTR 50.0%
AGGCAGAGCCAAAAGCAAGA+CGG + chr2_4:4684080-4684099 None:intergenic 50.0%
AGTACCCTCCAAGTCAGCTT+TGG + chr2_4:4684285-4684304 None:intergenic 50.0%
! GCCATGATAAAGGTGATGGC+TGG + chr2_4:4684551-4684570 None:intergenic 50.0%
! CTGATGCCACGTGGCTATTT+TGG + chr2_4:4685278-4685297 None:intergenic 50.0%
!! GCATCAGTCCATGCTGACTT+TGG - chr2_4:4685291-4685310 Msa0309900:intron 50.0%
GAATATCAGGCTACTAGCCG+TGG - chr2_4:4685973-4685992 Msa0309900:intron 50.0%
! GGTTGTTGCCTCTGGATTAG+TGG - chr2_4:4686107-4686126 Msa0309900:intron 50.0%
!! GAGCAGTGCTGATTGTGTGT+GGG - chr2_4:4686552-4686571 Msa0309900:intron 50.0%
!! AGAGGCCAAAGCTGACTTGG+AGG - chr2_4:4684277-4684296 Msa0309900:CDS 55.0%
!! GAGGCCAAAGCTGACTTGGA+GGG - chr2_4:4684278-4684297 Msa0309900:CDS 55.0%
GTTGGGTGTTCTGTGTGCAG+TGG - chr2_4:4685323-4685342 Msa0309900:intron 55.0%
GGAGCCAATGGAAGTTGGGT+TGG - chr2_4:4685951-4685970 Msa0309900:intron 55.0%
GCTACTAGCCGTGGCTTACT+CGG - chr2_4:4685982-4686001 Msa0309900:intron 55.0%
TTGTAGGGGGTTGTTGCCTC+TGG - chr2_4:4686099-4686118 Msa0309900:intron 55.0%
!! GGAGCAGTGCTGATTGTGTG+TGG - chr2_4:4686551-4686570 Msa0309900:intron 55.0%
GAGAGCAAGGGGAACGGTGA+AGG + chr2_4:4684100-4684119 None:intergenic 60.0%
GGAGCTTCTACGGTGCAGTC+AGG + chr2_4:4685069-4685088 None:intergenic 60.0%
! CAGCATGGACTGATGCCACG+TGG + chr2_4:4685287-4685306 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 4683861 4687067 4683861 ID=Msa0309900;Name=Msa0309900
chr2_4 mRNA 4683861 4687067 4683861 ID=Msa0309900-mRNA-1;Parent=Msa0309900;Name=Msa0309900-mRNA-1;_AED=0.18;_eAED=0.18;_QI=76|1|1|1|1|1|7|253|405
chr2_4 exon 4683861 4684384 4683861 ID=Msa0309900-mRNA-1:exon:1602;Parent=Msa0309900-mRNA-1
chr2_4 exon 4684672 4684823 4684672 ID=Msa0309900-mRNA-1:exon:1601;Parent=Msa0309900-mRNA-1
chr2_4 exon 4684925 4685083 4684925 ID=Msa0309900-mRNA-1:exon:1600;Parent=Msa0309900-mRNA-1
chr2_4 exon 4685545 4685806 4685545 ID=Msa0309900-mRNA-1:exon:1599;Parent=Msa0309900-mRNA-1
chr2_4 exon 4686346 4686462 4686346 ID=Msa0309900-mRNA-1:exon:1598;Parent=Msa0309900-mRNA-1
chr2_4 exon 4686594 4686656 4686594 ID=Msa0309900-mRNA-1:exon:1597;Parent=Msa0309900-mRNA-1
chr2_4 exon 4686798 4687067 4686798 ID=Msa0309900-mRNA-1:exon:1596;Parent=Msa0309900-mRNA-1
chr2_4 five_prime_UTR 4686992 4687067 4686992 ID=Msa0309900-mRNA-1:five_prime_utr;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4686798 4686991 4686798 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4686594 4686656 4686594 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4686346 4686462 4686346 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4685545 4685806 4685545 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4684925 4685083 4684925 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4684672 4684823 4684672 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 CDS 4684114 4684384 4684114 ID=Msa0309900-mRNA-1:cds;Parent=Msa0309900-mRNA-1
chr2_4 three_prime_UTR 4683861 4684113 4683861 ID=Msa0309900-mRNA-1:three_prime_utr;Parent=Msa0309900-mRNA-1
Gene Sequence

>Msa0309900

ATGAAGGGGGTATGGAATGGAGTACATGGTTTGAAACCAGTGATGCTTATGGTGTTGGTTCAAATTGCATATGCTGCTGTCAATGTGCTCTACAAACTTGCCATCAATGATGGAATGACTGTTAAAGTTGCTACTGCTTACCGTCTTGCTTTTGGCTCTGCCTTCACCGTTCCCCTTGCTCTCATTTCTGAAAGGAATAAGAGGCCAAAGCTGACTTGGAGGGTACTTTTCATGGCATTTCTTTGTGGCTTGTTTGGGGGAAGTTTATTTCAAAACCTTTTCTATGAGGCTTTGGCTTTAACATCAGCAACATTTGCATCAGCTATCTACAACCTCATTCCAGCCATCACCTTTATCATGGCCATCTCATGCGGTTTCGAAAGATTAAATTTGAGGGCTGCAGCTGGAAAAGCCAAAGTGTTGGGAACACTAATTGGAATTGGTGGGGCAATGATGTTGATATTTCTCAAAGGTTTAGAAATTAATATCTGGCCATTCCACATAAACCTTATGCATCCACATCAACACCAAAATAGCCACGTGGCATCAGTCCATGCTGACTTTGGTAGTAAATGGTTGGGTGTTCTGTGTGCAGTGGCAAGTTGCTTCTCTTTTGCTTTATGGCTCATAATTCAGGCTAAGATGAGCAAAGAATATCCAAGTCATTACTCAAGTACAGCTTTAATGTCTACAATGGGAGCAATACAAGCCACTGCTTTTGGTCTTTGTGTTGAAAGAGATTGGAGCCAATGGAAGTTGGGTTGGAATATCAGGCTACTAGCCGTGGCTTACTCGGGGGTTGTTGCCTCTGGATTAGTGGTTATTGTGACTTCATGGTGCATAAAGATGAGGGGGCCTTTATTTGCATCTGTCTTCAACCCTCTAATGCTATTGTTTGTCACTATTGTCGCATCTTTGATGTTGGATGAAAAATTATATTTAGGAAGTGCAATTGGAGCAGTGCTGATTGTGTGTGGGTTGTACATGGTTTTGTGGGGTAAGAGTAAAGAGATGAAAAAGATTGCTCAGTTGGTGCCATCAAAAAACACCCAAGAAGCTGAAGCTATACAAGTTGTTGTTATGTCGACACCAATAGTAGAGGATCATGATAAAGTACATGCCTACAACTATCACAACGAAGTTGATAAAGATCATGTAGATAATTTGTCAAAAAATGGAGAAATTAATAGTATTGGGGGTGCAGAAGTAGTGAAATGA
Protein sequence

>Msa0309900

MKGVWNGVHGLKPVMLMVLVQIAYAAVNVLYKLAINDGMTVKVATAYRLAFGSAFTVPLALISERNKRPKLTWRVLFMAFLCGLFGGSLFQNLFYEALALTSATFASAIYNLIPAITFIMAISCGFERLNLRAAAGKAKVLGTLIGIGGAMMLIFLKGLEINIWPFHINLMHPHQHQNSHVASVHADFGSKWLGVLCAVASCFSFALWLIIQAKMSKEYPSHYSSTALMSTMGAIQATAFGLCVERDWSQWKLGWNIRLLAVAYSGVVASGLVVIVTSWCIKMRGPLFASVFNPLMLLFVTIVASLMLDEKLYLGSAIGAVLIVCGLYMVLWGKSKEMKKIAQLVPSKNTQEAEAIQVVVMSTPIVEDHDKVHAYNYHNEVDKDHVDNLSKNGEINSIGGAEVVK*