AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0318500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318500 MtrunA17_Chr2g0292291 71.105 353 53 4 1 304 1 353 5.28e-169 473
Msa0318500 MtrunA17_Chr2g0292261 67.429 350 65 4 4 304 3 352 1.01e-152 431
Msa0318500 MtrunA17_Chr2g0292241 58.074 353 96 3 4 304 3 355 1.17e-139 398
Msa0318500 MtrunA17_Chr2g0292181 58.120 351 97 3 4 304 12 362 7.31e-136 389
Msa0318500 MtrunA17_Chr2g0292271 61.032 349 84 5 7 304 6 353 7.01e-135 386
Msa0318500 MtrunA17_Chr2g0292211 55.714 350 106 3 4 304 14 363 6.35e-131 376
Msa0318500 MtrunA17_Chr2g0291751 56.125 351 103 4 4 303 3 353 1.62e-126 365
Msa0318500 MtrunA17_Chr2g0292111 57.100 331 97 2 4 289 3 333 7.39e-125 360
Msa0318500 MtrunA17_Chr2g0292251 58.710 310 83 2 23 287 21 330 4.83e-122 353
Msa0318500 MtrunA17_Chr2g0292151 55.891 331 101 2 4 289 3 333 6.21e-122 353
Msa0318500 MtrunA17_Chr2g0292091 51.149 348 117 5 4 304 3 344 1.77e-109 321
Msa0318500 MtrunA17_Chr2g0292281 72.294 231 33 4 95 304 17 237 2.29e-106 309
Msa0318500 MtrunA17_Chr2g0292101 50.716 349 118 6 4 304 3 345 1.33e-104 309
Msa0318500 MtrunA17_Chr1g0175561 49.191 309 111 3 24 286 18 326 2.68e-89 269
Msa0318500 MtrunA17_Chr7g0258811 48.534 307 112 3 26 286 65 371 2.20e-88 268
Msa0318500 MtrunA17_Chr8g0372601 40.924 303 133 3 30 287 30 331 6.47e-70 220
Msa0318500 MtrunA17_Chr4g0070951 41.667 324 138 5 13 287 9 330 8.26e-70 219
Msa0318500 MtrunA17_Chr5g0404721 40.066 302 135 3 30 286 30 330 1.50e-67 214
Msa0318500 MtrunA17_Chr5g0402571 41.503 306 128 6 28 286 25 326 1.17e-66 211
Msa0318500 MtrunA17_Chr7g0244461 37.117 326 144 5 6 286 3 312 1.70e-66 210
Msa0318500 MtrunA17_Chr1g0155611 39.735 302 137 2 30 286 46 347 2.56e-66 211
Msa0318500 MtrunA17_Chr4g0013201 38.511 309 136 4 23 286 39 338 3.47e-66 210
Msa0318500 MtrunA17_Chr2g0320091 39.935 308 130 6 25 286 20 318 3.87e-66 209
Msa0318500 MtrunA17_Chr5g0402581 40.000 305 134 5 28 286 25 326 6.52e-66 209
Msa0318500 MtrunA17_Chr4g0013171 38.994 318 138 6 16 286 13 321 1.31e-65 208
Msa0318500 MtrunA17_Chr4g0013191 44.015 259 116 4 48 286 91 340 8.41e-65 207
Msa0318500 MtrunA17_Chr2g0299691 39.739 307 135 4 29 287 55 359 1.33e-64 207
Msa0318500 MtrunA17_Chr4g0038151 38.298 329 145 6 6 286 3 321 1.60e-64 206
Msa0318500 MtrunA17_Chr4g0013181 41.196 301 121 6 33 286 62 353 2.72e-64 206
Msa0318500 MtrunA17_Chr2g0320061 38.111 307 137 4 25 286 20 318 3.55e-64 204
Msa0318500 MtrunA17_Chr2g0320071 37.117 326 148 5 9 286 1 317 7.34e-64 204
Msa0318500 MtrunA17_Chr2g0320051 36.842 323 151 4 9 286 4 318 2.39e-63 202
Msa0318500 MtrunA17_Chr7g0244451 40.461 304 127 4 28 286 22 316 3.19e-63 202
Msa0318500 MtrunA17_Chr3g0129051 38.272 324 140 6 6 282 8 318 3.78e-63 202
Msa0318500 MtrunA17_Chr2g0320101 38.111 307 136 4 25 286 20 317 8.76e-63 201
Msa0318500 MtrunA17_Chr4g0038171 39.610 308 131 5 27 286 21 321 6.46e-62 199
Msa0318500 MtrunA17_Chr4g0038161 38.298 329 144 6 6 286 3 320 1.17e-61 198
Msa0318500 MtrunA17_Chr4g0043391 37.725 334 150 6 1 286 1 324 1.70e-61 198
Msa0318500 MtrunA17_Chr2g0320031 37.582 306 136 5 27 286 23 319 2.97e-60 194
Msa0318500 MtrunA17_Chr3g0106921 37.654 324 141 8 11 286 10 320 5.76e-60 194
Msa0318500 MtrunA17_Chr3g0114451 36.634 303 138 4 29 286 25 318 9.22e-59 191
Msa0318500 MtrunA17_Chr5g0432341 38.188 309 135 7 28 288 23 323 1.21e-57 188
Msa0318500 MtrunA17_Chr3g0107881 36.446 332 153 8 4 286 2 324 4.24e-57 186
Msa0318500 MtrunA17_Chr5g0438021 35.737 319 145 6 15 286 20 325 1.10e-56 186
Msa0318500 MtrunA17_Chr4g0068621 38.672 256 136 3 49 286 84 336 1.21e-56 186
Msa0318500 MtrunA17_Chr4g0013721 35.831 307 147 5 25 286 95 396 1.32e-56 188
Msa0318500 MtrunA17_Chr5g0432351 37.126 334 149 8 1 286 1 321 1.91e-56 185
Msa0318500 MtrunA17_Chr5g0421411 39.695 262 135 6 48 287 26 286 2.75e-56 183
Msa0318500 MtrunA17_Chr3g0106901 35.873 315 148 4 17 286 18 323 6.36e-56 183
Msa0318500 MtrunA17_Chr3g0129041 37.386 329 144 7 6 286 8 322 2.37e-55 182
Msa0318500 MtrunA17_Chr2g0322861 36.792 318 150 7 16 286 73 386 3.88e-55 183
Msa0318500 MtrunA17_Chr3g0129061 36.111 324 147 6 6 282 8 318 6.41e-55 181
Msa0318500 MtrunA17_Chr5g0432441 36.420 324 148 6 12 286 12 326 1.31e-54 180
Msa0318500 MtrunA17_Chr1g0213061 33.750 320 156 6 14 286 11 321 1.61e-54 180
Msa0318500 MtrunA17_Chr5g0400331 35.644 303 146 4 33 286 29 331 1.73e-54 180
Msa0318500 MtrunA17_Chr4g0024121 34.743 331 161 7 2 286 5 326 2.29e-54 179
Msa0318500 MtrunA17_Chr5g0401431 35.366 328 158 7 7 285 6 328 2.60e-54 179
Msa0318500 MtrunA17_Chr5g0432331 37.748 302 146 6 28 288 54 354 1.18e-53 178
Msa0318500 MtrunA17_Chr4g0013821 34.768 302 147 5 30 286 28 324 1.48e-53 178
Msa0318500 MtrunA17_Chr1g0204011 35.621 306 146 6 26 286 23 322 3.95e-52 174
Msa0318500 MtrunA17_Chr5g0432401 36.393 305 135 5 12 286 11 286 4.21e-52 172
Msa0318500 MtrunA17_Chr4g0076211 36.667 300 134 8 35 288 46 335 6.15e-52 174
Msa0318500 MtrunA17_Chr3g0128061 33.639 327 166 5 7 287 6 327 9.31e-52 173
Msa0318500 MtrunA17_Chr7g0268501 35.625 320 155 6 12 286 7 320 5.79e-51 171
Msa0318500 MtrunA17_Chr7g0268521 35.625 320 155 6 12 286 7 320 5.79e-51 171
Msa0318500 MtrunA17_Chr7g0225221 35.370 311 142 6 25 286 20 320 1.34e-49 167
Msa0318500 MtrunA17_Chr8g0371381 34.516 310 144 10 25 286 21 319 1.44e-48 164
Msa0318500 MtrunA17_Chr7g0224371 30.395 329 174 5 3 286 4 322 3.46e-48 163
Msa0318500 MtrunA17_Chr1g0148021 33.443 305 154 4 29 286 26 328 5.28e-48 163
Msa0318500 MtrunA17_Chr5g0405991 37.597 258 133 8 48 286 68 316 7.33e-48 162
Msa0318500 MtrunA17_Chr7g0254201 32.895 304 150 7 28 286 18 312 1.95e-47 161
Msa0318500 MtrunA17_Chr4g0053871 36.452 310 138 8 23 282 20 320 5.40e-47 160
Msa0318500 MtrunA17_Chr4g0063671 33.750 320 155 8 17 286 8 320 6.90e-47 160
Msa0318500 MtrunA17_Chr1g0194271 31.579 342 173 7 5 288 4 342 9.63e-47 160
Msa0318500 MtrunA17_Chr1g0194151 32.036 334 171 7 5 286 4 333 1.42e-46 159
Msa0318500 MtrunA17_Chr6g0477071 33.133 332 157 12 6 286 25 342 3.44e-45 156
Msa0318500 MtrunA17_Chr4g0072091 34.754 305 140 6 29 282 23 319 5.68e-45 155
Msa0318500 MtrunA17_Chr6g0467161 32.286 350 149 10 9 287 5 337 5.91e-45 155
Msa0318500 MtrunA17_Chr2g0279041 34.768 302 141 7 34 286 31 325 6.99e-45 155
Msa0318500 MtrunA17_Chr4g0075241 31.627 332 176 6 1 286 1 327 9.56e-45 154
Msa0318500 MtrunA17_Chr2g0296621 34.333 300 140 9 33 286 53 341 1.29e-43 152
Msa0318500 MtrunA17_Chr2g0279061 36.882 263 137 6 46 286 11 266 5.92e-43 148
Msa0318500 MtrunA17_Chr1g0197411 31.657 338 175 9 1 288 1 332 9.23e-43 149
Msa0318500 MtrunA17_Chr1g0157051 32.237 304 150 7 30 286 24 318 1.48e-42 149
Msa0318500 MtrunA17_Chr8g0364291 30.699 329 178 6 7 288 1 326 4.96e-42 147
Msa0318500 MtrunA17_Chr1g0164891 31.126 302 157 6 34 286 93 392 1.16e-41 148
Msa0318500 MtrunA17_Chr4g0046131 31.803 305 156 7 29 283 41 343 1.25e-41 147
Msa0318500 MtrunA17_Chr1g0188431 33.113 302 144 9 30 286 39 327 2.10e-41 146
Msa0318500 MtrunA17_Chr6g0467121 34.202 307 135 11 30 286 74 363 4.77e-40 144
Msa0318500 MtrunA17_Chr3g0136751 33.333 261 148 4 48 288 70 324 9.24e-40 141
Msa0318500 MtrunA17_Chr6g0467251 33.887 301 139 10 30 282 36 324 3.50e-39 140
Msa0318500 MtrunA17_Chr7g0254231 32.013 303 149 9 30 287 22 312 5.67e-39 139
Msa0318500 MtrunA17_Chr2g0316791 30.966 352 157 10 15 283 55 403 2.06e-38 140
Msa0318500 MtrunA17_Chr2g0316481 29.830 352 161 9 15 283 55 403 1.19e-37 138
Msa0318500 MtrunA17_Chr2g0311251 31.325 332 149 8 30 285 11 339 9.51e-37 134
Msa0318500 MtrunA17_Chr2g0329641 29.070 344 173 12 1 286 5 335 9.84e-37 134
Msa0318500 MtrunA17_Chr2g0278671 31.833 311 155 9 26 286 20 323 3.56e-36 132
Msa0318500 MtrunA17_Chr2g0316191 30.028 353 160 10 15 283 55 404 5.05e-36 133
Msa0318500 MtrunA17_Chr2g0316831 29.830 352 161 9 15 283 91 439 7.20e-36 134
Msa0318500 MtrunA17_Chr6g0460711 30.650 323 170 8 9 286 1 314 1.28e-34 128
Msa0318500 MtrunA17_Chr6g0451571 29.677 310 164 7 26 286 30 334 3.62e-34 127
Msa0318500 MtrunA17_Chr5g0413051 28.939 311 167 8 27 286 70 377 5.97e-33 124
Msa0318500 MtrunA17_Chr2g0316141 32.836 268 144 6 49 283 11 275 1.32e-32 121
Msa0318500 MtrunA17_Chr2g0316181 32.836 268 144 6 49 283 11 275 1.32e-32 121
Msa0318500 MtrunA17_Chr4g0004591 30.976 297 152 6 27 285 74 355 3.12e-32 122
Msa0318500 MtrunA17_Chr2g0311241 27.919 394 156 11 16 285 40 429 4.21e-32 123
Msa0318500 MtrunA17_Chr1g0181511 29.221 308 162 7 28 287 25 324 1.30e-31 120
Msa0318500 MtrunA17_Chr3g0114461 33.684 190 100 3 115 286 11 192 6.35e-30 112
Msa0318500 MtrunA17_Chr1g0181431 28.571 308 164 6 28 287 25 324 6.87e-30 115
Msa0318500 MtrunA17_Chr1g0181481 28.990 307 162 6 28 286 25 323 9.96e-30 115
Msa0318500 MtrunA17_Chr4g0074851 27.483 302 163 6 33 286 35 328 1.49e-28 112
Msa0318500 MtrunA17_Chr2g0316811 30.078 256 110 5 15 202 29 283 3.42e-26 105
Msa0318500 MtrunA17_Chr3g0114491 42.727 110 36 1 29 111 17 126 4.77e-19 81.6
Msa0318500 MtrunA17_Chr3g0104101 36.154 130 78 5 162 287 22 150 1.03e-15 73.2
Msa0318500 MtrunA17_Chr5g0432361 38.372 86 52 1 200 285 24 108 5.04e-13 64.3
Msa0318500 MtrunA17_Chr7g0254181 33.333 108 69 1 50 157 6 110 2.52e-12 63.9
Msa0318500 MtrunA17_Chr7g0254171 31.405 121 76 4 48 163 3 121 1.77e-11 60.8
Msa0318500 MtrunA17_Chr1g0196191 52.308 65 28 2 49 111 31 94 5.63e-11 60.1
Msa0318500 MtrunA17_Chr5g0432321 31.452 124 59 3 124 229 17 132 8.11e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318500 AT5G06720.1 47.003 317 123 2 15 286 17 333 4.40e-91 274
Msa0318500 AT5G06730.1 45.697 337 134 3 15 302 18 354 7.92e-91 275
Msa0318500 AT2G38380.1 45.988 324 127 3 26 303 27 348 2.16e-88 268
Msa0318500 AT2G38390.1 45.062 324 130 3 26 303 27 348 2.08e-86 263
Msa0318500 AT3G49120.1 45.399 326 131 3 26 304 28 353 2.01e-84 258
Msa0318500 AT3G32980.1 45.092 326 132 3 26 304 27 352 8.75e-83 254
Msa0318500 AT3G49110.1 45.092 326 132 3 26 304 29 354 7.44e-82 251
Msa0318500 AT4G08770.1 43.887 319 132 4 26 297 20 338 1.71e-79 245
Msa0318500 AT4G08780.1 42.638 326 140 3 26 304 20 345 3.38e-79 244
Msa0318500 AT5G66390.1 38.024 334 158 4 1 286 1 333 1.55e-70 222
Msa0318500 AT5G19890.1 42.157 306 124 6 28 286 28 327 8.28e-69 217
Msa0318500 AT1G44970.1 39.871 311 140 3 21 286 38 346 4.72e-67 213
Msa0318500 AT5G19880.1 40.446 314 137 7 22 286 17 329 1.48e-66 211
Msa0318500 AT4G36430.1 37.662 308 146 3 25 287 25 331 1.35e-64 206
Msa0318500 AT5G05340.1 39.869 306 128 5 28 286 28 324 2.23e-64 206
Msa0318500 AT3G50990.1 39.088 307 141 3 25 286 36 341 5.46e-64 205
Msa0318500 AT1G68850.1 39.088 307 132 8 33 287 33 336 7.25e-63 202
Msa0318500 AT2G18150.1 37.748 302 142 3 30 286 36 336 1.23e-62 201
Msa0318500 AT1G14550.1 38.889 306 134 5 27 286 23 321 5.20e-62 199
Msa0318500 AT1G49570.1 38.033 305 139 5 28 284 46 348 3.66e-61 198
Msa0318500 AT2G18140.1 37.417 302 143 3 30 286 35 335 4.59e-61 197
Msa0318500 AT2G22420.1 35.276 326 154 6 6 286 6 319 1.60e-58 191
Msa0318500 AT1G14540.1 35.385 325 148 5 7 286 8 315 9.53e-58 188
Msa0318500 AT4G16270.1 36.093 302 145 4 30 286 50 348 2.84e-57 188
Msa0318500 AT2G18980.1 36.196 326 152 6 10 286 5 323 1.94e-55 182
Msa0318500 AT5G67400.1 36.446 332 154 7 9 286 1 329 9.78e-55 181
Msa0318500 AT1G71695.1 37.742 310 138 7 30 292 44 345 2.18e-54 181
Msa0318500 AT5G14130.1 37.107 318 146 7 19 286 17 330 3.91e-54 179
Msa0318500 AT4G37530.1 37.217 309 144 5 26 286 23 329 5.37e-54 179
Msa0318500 AT5G14130.2 37.107 318 146 7 19 286 58 371 9.97e-54 179
Msa0318500 AT4G30170.1 36.246 309 141 7 27 286 24 325 2.59e-53 177
Msa0318500 AT3G03670.1 33.127 323 167 5 9 286 3 321 9.49e-53 176
Msa0318500 AT3G49960.1 34.954 329 159 7 7 286 7 329 2.81e-52 174
Msa0318500 AT3G21770.1 35.556 315 154 6 19 287 18 329 1.35e-51 173
Msa0318500 AT5G58390.1 35.484 310 141 8 26 286 17 316 1.37e-51 172
Msa0318500 AT5G58400.1 35.938 320 143 8 16 286 19 325 2.58e-51 172
Msa0318500 AT5G17820.1 33.230 322 157 8 10 286 5 313 5.38e-51 171
Msa0318500 AT2G35380.1 37.107 318 147 6 18 286 22 335 2.07e-50 170
Msa0318500 AT4G11290.1 34.675 323 161 6 11 286 7 326 2.96e-50 169
Msa0318500 AT5G17820.2 33.230 322 157 8 10 286 58 366 5.34e-50 170
Msa0318500 AT1G05260.1 34.853 307 149 7 28 287 24 326 1.32e-49 167
Msa0318500 AT5G51890.1 35.714 336 152 12 1 286 1 322 1.88e-49 167
Msa0318500 AT4G37520.1 35.505 307 148 5 28 286 25 329 2.18e-49 167
Msa0318500 AT5G15180.1 33.735 332 166 8 1 286 6 329 4.21e-49 166
Msa0318500 AT3G01190.1 33.735 332 163 10 1 286 1 321 6.46e-49 166
Msa0318500 AT5G64120.1 33.832 334 159 9 4 286 6 328 2.77e-48 164
Msa0318500 AT4G37520.2 35.179 307 146 6 28 286 25 326 6.31e-48 163
Msa0318500 AT2G41480.1 35.604 323 147 10 15 286 16 328 5.17e-47 161
Msa0318500 AT5G39580.1 34.043 329 156 9 4 286 6 319 2.22e-46 159
Msa0318500 AT4G26010.1 33.766 308 143 8 25 286 17 309 3.02e-44 153
Msa0318500 AT4G26010.2 33.766 308 143 8 25 286 45 337 5.15e-44 153
Msa0318500 AT2G35380.2 39.676 247 127 4 62 286 1 247 1.25e-43 150
Msa0318500 AT4G37530.2 36.965 257 114 4 26 234 23 279 4.59e-43 149
Msa0318500 AT4G17690.1 32.362 309 153 7 30 290 26 326 6.81e-42 147
Msa0318500 AT1G05240.1 32.258 310 147 8 30 286 26 325 7.17e-42 147
Msa0318500 AT1G05250.1 32.258 310 147 8 30 286 26 325 7.17e-42 147
Msa0318500 AT4G33870.1 29.290 338 154 7 27 283 65 398 1.11e-41 149
Msa0318500 AT5G42180.1 31.230 317 163 9 15 285 9 316 1.02e-40 144
Msa0318500 AT5G64110.1 34.437 302 140 8 34 286 38 330 1.09e-40 144
Msa0318500 AT2G34060.1 31.596 307 158 7 27 283 39 343 1.49e-39 142
Msa0318500 AT2G39040.1 33.333 312 152 8 27 286 43 350 2.66e-39 141
Msa0318500 AT4G33420.1 31.269 323 163 10 9 286 17 325 3.23e-39 140
Msa0318500 AT2G43480.1 30.321 343 168 11 7 286 1 335 3.86e-39 140
Msa0318500 AT4G25980.1 31.595 326 163 8 11 286 11 326 6.08e-39 140
Msa0318500 AT4G33420.2 31.269 323 163 10 9 286 64 372 7.25e-39 140
Msa0318500 AT1G34510.1 31.148 305 149 7 28 286 20 309 3.01e-38 137
Msa0318500 AT5G24070.1 34.586 266 133 9 49 286 83 335 5.17e-38 137
Msa0318500 AT5G64100.1 31.788 302 148 8 33 286 40 331 2.41e-37 135
Msa0318500 AT1G30870.1 33.077 260 143 7 49 287 97 346 7.20e-37 135
Msa0318500 AT5G22410.1 29.022 317 166 10 17 283 15 322 9.82e-37 134
Msa0318500 AT4G31760.1 28.955 335 180 7 1 286 1 326 9.82e-36 131
Msa0318500 AT1G77100.1 31.429 315 147 8 26 286 20 319 9.91e-36 131
Msa0318500 AT5G47000.1 29.260 311 165 7 28 290 31 334 6.16e-35 129
Msa0318500 AT2G24800.1 30.619 307 159 6 28 286 29 329 1.03e-34 129
Msa0318500 AT5G40150.1 29.032 310 166 6 24 286 26 328 4.93e-34 127
Msa0318500 AT1G24110.1 30.462 325 170 10 11 286 5 322 1.46e-33 125
Msa0318500 AT3G28200.1 28.939 311 162 8 25 286 16 316 1.27e-32 123
Msa0318500 AT2G37130.1 30.097 309 159 8 27 286 27 327 7.24e-32 121
Msa0318500 AT3G17070.1 29.882 338 172 11 3 286 13 339 9.08e-31 118
Msa0318500 AT2G37130.2 30.033 303 155 8 33 286 2 296 1.87e-30 116
Msa0318500 AT4G21960.1 27.961 304 159 7 33 286 34 327 1.03e-29 115
Msa0318500 AT3G17070.2 32.453 265 143 9 49 286 21 276 9.42e-27 106
Msa0318500 AT4G25980.2 35.632 174 77 5 11 157 56 221 6.43e-21 90.9
Msa0318500 AT5G39580.2 36.145 166 67 5 4 142 6 159 1.59e-19 85.9
Msa0318500 AT2G24800.2 29.299 157 80 2 28 157 29 181 3.10e-14 71.2

Find 66 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGCCTCTATGATTAAAA+TGG 0.131743 2_4:-17499904 Msa0318500:CDS
ATGATTAAAATGGGTAATAT+TGG 0.284100 2_4:-17499894 Msa0318500:CDS
CGCTTGTGGAAAACAATTCT+TGG 0.287458 2_4:+17499991 None:intergenic
CACAACATCCAAACCTCTTA+TGG 0.297023 2_4:+17501779 None:intergenic
TGCTAATATTTCCACAGTTC+TGG 0.297066 2_4:-17501685 Msa0318500:intron
TAATAGCACACTCACCAATT+TGG 0.298613 2_4:-17500090 Msa0318500:CDS
AAACTATCTCTTCTTCCTAA+TGG 0.298706 2_4:+17500796 None:intergenic
TGCAGGCTCTTGGTCCTGAT+TGG 0.348768 2_4:-17500825 Msa0318500:intron
TTAGCTGAGTAAGATCAAAT+TGG 0.354643 2_4:+17500731 None:intergenic
AAGCTTCAAAGAAAAGAGTT+TGG 0.356755 2_4:+17499928 None:intergenic
GCAGGCTCTTGGTCCTGATT+GGG 0.385775 2_4:-17500824 Msa0318500:intron
CTTGCTAATTCAAACCTTCC+TGG 0.385977 2_4:-17500757 Msa0318500:CDS
TAGCTGAGTAAGATCAAATT+GGG 0.393030 2_4:+17500732 None:intergenic
TAGCTCGAGTCGAATGTATC+TGG 0.415232 2_4:+17500056 None:intergenic
TGACATAATTTGCAGGCTCT+TGG 0.415627 2_4:-17500835 Msa0318500:intron
TGCTGTGTAGTGGTTGTGCT+TGG 0.416566 2_4:-17502310 Msa0318500:CDS
TTGACAATGGAAGTGACATT+TGG 0.425363 2_4:+17502229 None:intergenic
CCTGATTGGGAAGTTCCATT+AGG 0.430222 2_4:-17500811 Msa0318500:CDS
AGAACAATATGTCCCATTGA+TGG 0.430802 2_4:-17500116 Msa0318500:CDS
TGTTCTCAATGTTTGTAAGT+AGG 0.437059 2_4:+17500133 None:intergenic
ACCATCTTCCTTGGTGACTT+TGG 0.437925 2_4:+17499803 None:intergenic
TGGAGTGGTTGGGTCCAAAT+TGG 0.439167 2_4:+17500076 None:intergenic
CTCAAACCTAAGGATTCAAA+AGG 0.440527 2_4:-17500030 Msa0318500:CDS
AAGGCCTCTATGATTAAAAT+GGG 0.441216 2_4:-17499903 Msa0318500:CDS
CCTAATGGAACTTCCCAATC+AGG 0.449764 2_4:+17500811 None:intergenic
TCGAATGTATCTGGAGTGGT+TGG 0.452877 2_4:+17500065 None:intergenic
CAAGTGTCCTTGTAGAAGTT+AGG 0.456671 2_4:+17502253 None:intergenic
TGTGTAGTGGTTGTGCTTGG+AGG 0.472368 2_4:-17502307 Msa0318500:CDS
AAGTAGGTTGTGTTAAGAGT+TGG 0.473975 2_4:+17500149 None:intergenic
CCTTGGTGACTTTGGTAGCA+AGG 0.480830 2_4:+17499811 None:intergenic
AAATTGGTGAGTGTGCTATT+AGG 0.482129 2_4:+17500092 None:intergenic
GTGTAGTGGTTGTGCTTGGA+GGG 0.502187 2_4:-17502306 Msa0318500:CDS
GGTTGTGTTAAGAGTTGGAT+CGG 0.505458 2_4:+17500154 None:intergenic
TGTGCGGATATTCTTGCACT+TGG 0.517442 2_4:-17501708 Msa0318500:CDS
GATACTATCGTGAGCGAGCA+AGG 0.535008 2_4:-17501822 Msa0318500:CDS
GTTGAGACCTTGTTTAGCAA+AGG 0.535900 2_4:+17500702 None:intergenic
CGAATGTATCTGGAGTGGTT+GGG 0.542591 2_4:+17500066 None:intergenic
GTTGTGTTAAGAGTTGGATC+GGG 0.543829 2_4:+17500155 None:intergenic
AAGTTGTGCATTTGAAGAGA+AGG 0.544272 2_4:+17502279 None:intergenic
ACCAAAGTCACCAAGGAAGA+TGG 0.548166 2_4:-17499804 Msa0318500:CDS
AGTTGTGCATTTGAAGAGAA+GGG 0.549134 2_4:+17502280 None:intergenic
GGCAGCTTTGTGCTGTGTAG+TGG 0.549901 2_4:-17502320 Msa0318500:CDS
AGTGGTGTTTGCGCCGCTTG+TGG 0.552455 2_4:+17499977 None:intergenic
CGAGCTACTACTCAAACCTA+AGG 0.553925 2_4:-17500040 Msa0318500:CDS
GTGCTATTAGGTCCATCAAT+GGG 0.561855 2_4:+17500104 None:intergenic
GCTAACAGTACCATCTTCCT+TGG 0.566259 2_4:+17499794 None:intergenic
GAGACCTTGTTTAGCAAAGG+AGG 0.569485 2_4:+17500705 None:intergenic
TGCTAAGACTTGTTTGACAA+TGG 0.574005 2_4:+17502216 None:intergenic
TGTGCTATTAGGTCCATCAA+TGG 0.576345 2_4:+17500103 None:intergenic
GTAAGATCAAATTGGGGACC+AGG 0.580024 2_4:+17500739 None:intergenic
TCGTGAGCGAGCAAGGAGCT+AGG 0.582411 2_4:-17501815 Msa0318500:CDS
TCAAACCTAAGGATTCAAAA+GGG 0.596776 2_4:-17500029 Msa0318500:CDS
ATATCCTCCTTTGCTAAACA+AGG 0.599718 2_4:-17500709 Msa0318500:intron
TCCTAGTGTTGTGTCTTGTG+CGG 0.602765 2_4:-17501724 Msa0318500:CDS
ACTTGATCCTAACTTCTACA+AGG 0.616898 2_4:-17502260 Msa0318500:CDS
GATCAAATTGGGGACCAGGA+AGG 0.621129 2_4:+17500743 None:intergenic
GAAACTATTGACAATGGCAG+TGG 0.640788 2_4:+17499959 None:intergenic
GTTGTTGAAACTATTGACAA+TGG 0.651873 2_4:+17499953 None:intergenic
CGAGTCGAATGTATCTGGAG+TGG 0.656528 2_4:+17500061 None:intergenic
TGTGGAACATACCAGAACTG+TGG 0.673152 2_4:+17501674 None:intergenic
GGAGTGTTAAATGAAACTCA+AGG 0.673821 2_4:-17499873 Msa0318500:CDS
GAATCAAATCAAAGACGCAG+TGG 0.676892 2_4:-17501757 Msa0318500:CDS
ACAATTGGTAGAGGTCAATG+TGG 0.678112 2_4:-17500221 Msa0318500:intron
TCCGCACAAGACACAACACT+AGG 0.691012 2_4:+17501723 None:intergenic
CCTTGCTACCAAAGTCACCA+AGG 0.693533 2_4:-17499811 Msa0318500:CDS
AGCTGAGTAAGATCAAATTG+GGG 0.702266 2_4:+17500733 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTATTAATTTTTATTT+TGG - chr2_4:17501703-17501722 Msa0318500:CDS 0.0%
!! TAAGAAGAAATTTAATTATA+AGG + chr2_4:17500729-17500748 None:intergenic 10.0%
!!! TTTTGTTATAATTTTAGTTT+TGG - chr2_4:17500992-17501011 Msa0318500:intron 10.0%
!! TCTAAAAACATTACTAAATT+TGG - chr2_4:17500218-17500237 Msa0318500:intron 15.0%
!! ATATATGTTATATCTATGTA+AGG - chr2_4:17500254-17500273 Msa0318500:intron 15.0%
!! TATATGTTATATCTATGTAA+GGG - chr2_4:17500255-17500274 Msa0318500:intron 15.0%
!! TTAAAAAATTAACAGAAACA+TGG + chr2_4:17500694-17500713 None:intergenic 15.0%
!!! AAGTTGATCTTTTTATATTT+AGG + chr2_4:17500943-17500962 None:intergenic 15.0%
!! TATTAAAAGGAATTAAAGAA+AGG + chr2_4:17501144-17501163 None:intergenic 15.0%
!!! GTTTTTATAACTATTATACT+AGG - chr2_4:17501232-17501251 Msa0318500:intron 15.0%
!!! TAAACTTTTAACAAAATCAC+AGG + chr2_4:17500097-17500116 None:intergenic 20.0%
!! TTGGTGCATATTTAAATTTA+TGG + chr2_4:17500163-17500182 None:intergenic 20.0%
!! AAATATATTACTCTTCGTAT+TGG + chr2_4:17500182-17500201 None:intergenic 20.0%
!! TTTAGATTTGTAGTATATGA+TGG + chr2_4:17500616-17500635 None:intergenic 20.0%
!!! CTATACATTTTTTAGTACTA+TGG + chr2_4:17500888-17500907 None:intergenic 20.0%
!! CATAGTACTAAAAAATGTAT+AGG - chr2_4:17500886-17500905 Msa0318500:intron 20.0%
!! TTAAGACTAAAATCAACAAT+AGG + chr2_4:17501074-17501093 None:intergenic 20.0%
!! AATATAAAGAGTAGACTAAA+AGG - chr2_4:17501092-17501111 Msa0318500:intron 20.0%
!!! ATTAAAGAAAGGCATTTTTT+AGG + chr2_4:17501133-17501152 None:intergenic 20.0%
!!! CCAATTTTTGCTTATATTTT+GGG + chr2_4:17501621-17501640 None:intergenic 20.0%
!!! ACCAATTTTTGCTTATATTT+TGG + chr2_4:17501620-17501639 None:intergenic 20.0%
!!! ACCAATTTTTGCTTATATTT+TGG - chr2_4:17501620-17501639 Msa0318500:intron 20.0%
!!! CCAATTTTTGCTTATATTTT+GGG - chr2_4:17501621-17501640 Msa0318500:intron 20.0%
!! ATGATTAAAATGGGTAATAT+TGG - chr2_4:17502237-17502256 Msa0318500:CDS 20.0%
!! ACTTTAATAGTCTTACCATT+AGG - chr2_4:17500575-17500594 Msa0318500:intron 25.0%
!! ACAATAGGAAGAAATTTTGT+AGG + chr2_4:17501059-17501078 None:intergenic 25.0%
! AGTAATAATAATAAGTCGTG+AGG + chr2_4:17501180-17501199 None:intergenic 25.0%
!!! TTTTTGCTTATATTTTGGGA+CGG + chr2_4:17501625-17501644 None:intergenic 25.0%
! CCCAAAATATAAGCAAAAAT+TGG - chr2_4:17501539-17501558 Msa0318500:intron 25.0%
! TCAACAAAAGTTAATGTATC+TGG - chr2_4:17501562-17501581 Msa0318500:intron 25.0%
! TCAACGAAAGTTAATGTATT+TGG + chr2_4:17501601-17501620 None:intergenic 25.0%
! CCCAAAATATAAGCAAAAAT+TGG + chr2_4:17501539-17501558 None:intergenic 25.0%
!!! TTTTTGCTTATATTTTGGGA+CGG - chr2_4:17501625-17501644 Msa0318500:intron 25.0%
!! TATATTTCTACATATGTGTG+CGG - chr2_4:17501658-17501677 Msa0318500:intron 25.0%
!!! TCTTTTCTTTGAAGCTTTTA+AGG - chr2_4:17502209-17502228 Msa0318500:intron 25.0%
!!! ATTACCCATTTTAATCATAG+AGG + chr2_4:17502235-17502254 None:intergenic 25.0%
! CTCTTCTTTTCATCTCATAT+TGG - chr2_4:17499790-17499809 Msa0318500:CDS 30.0%
TGATTCTATCAAACTCATTG+TGG + chr2_4:17500067-17500086 None:intergenic 30.0%
! CCTCTTATGGAGTTTTTATT+TGG + chr2_4:17500342-17500361 None:intergenic 30.0%
CCAAATAAAAACTCCATAAG+AGG - chr2_4:17500339-17500358 Msa0318500:intron 30.0%
TAAAAACTCCATAAGAGGTT+TGG - chr2_4:17500344-17500363 Msa0318500:intron 30.0%
TTTCTCTGATGTCATCAAAA+AGG + chr2_4:17500667-17500686 None:intergenic 30.0%
!! ACTTTCAAGTTACTTACAGA+TGG + chr2_4:17500915-17500934 None:intergenic 30.0%
AAACTATCTCTTCTTCCTAA+TGG + chr2_4:17501338-17501357 None:intergenic 30.0%
TAGCTGAGTAAGATCAAATT+GGG + chr2_4:17501402-17501421 None:intergenic 30.0%
TTAGCTGAGTAAGATCAAAT+TGG + chr2_4:17501403-17501422 None:intergenic 30.0%
CAATTTACAACTGTCTCTAA+CGG - chr2_4:17501771-17501790 Msa0318500:CDS 30.0%
AATTTACAACTGTCTCTAAC+GGG - chr2_4:17501772-17501791 Msa0318500:CDS 30.0%
GGATTTAATTGTGTCAATTG+AGG - chr2_4:17501793-17501812 Msa0318500:CDS 30.0%
CCACATATACATACATAATG+AGG + chr2_4:17501849-17501868 None:intergenic 30.0%
CCTCATTATGTATGTATATG+TGG - chr2_4:17501846-17501865 Msa0318500:CDS 30.0%
ATATTAGGTGCTCATACAAT+TGG - chr2_4:17501895-17501914 Msa0318500:intron 30.0%
TGTTCTCAATGTTTGTAAGT+AGG + chr2_4:17502001-17502020 None:intergenic 30.0%
TCAAACCTAAGGATTCAAAA+GGG - chr2_4:17502102-17502121 Msa0318500:intron 30.0%
GTTGTTGAAACTATTGACAA+TGG + chr2_4:17502181-17502200 None:intergenic 30.0%
AAGCTTCAAAGAAAAGAGTT+TGG + chr2_4:17502206-17502225 None:intergenic 30.0%
TAAGGCCTCTATGATTAAAA+TGG - chr2_4:17502227-17502246 Msa0318500:CDS 30.0%
AAGGCCTCTATGATTAAAAT+GGG - chr2_4:17502228-17502247 Msa0318500:CDS 30.0%
AGTTGTGCATTTGAAGAGAA+GGG + chr2_4:17499854-17499873 None:intergenic 35.0%
AAGTTGTGCATTTGAAGAGA+AGG + chr2_4:17499855-17499874 None:intergenic 35.0%
ACTTGATCCTAACTTCTACA+AGG - chr2_4:17499871-17499890 Msa0318500:CDS 35.0%
TTGACAATGGAAGTGACATT+TGG + chr2_4:17499905-17499924 None:intergenic 35.0%
! TGCTAAGACTTGTTTGACAA+TGG + chr2_4:17499918-17499937 None:intergenic 35.0%
AAAGTGAAGCCTAATGAAAC+TGG + chr2_4:17499972-17499991 None:intergenic 35.0%
TGCTAATATTTCCACAGTTC+TGG - chr2_4:17500446-17500465 Msa0318500:intron 35.0%
TAAGTCTCAGTTAGAAGATG+TGG + chr2_4:17500478-17500497 None:intergenic 35.0%
! AGTATATGATGGAATGAGCA+GGG + chr2_4:17500605-17500624 None:intergenic 35.0%
! TAGTATATGATGGAATGAGC+AGG + chr2_4:17500606-17500625 None:intergenic 35.0%
TTATGTCACCAGGAAATATG+AGG + chr2_4:17501284-17501303 None:intergenic 35.0%
AGCTGAGTAAGATCAAATTG+GGG + chr2_4:17501401-17501420 None:intergenic 35.0%
ATATCCTCCTTTGCTAAACA+AGG - chr2_4:17501422-17501441 Msa0318500:intron 35.0%
! GCGTACAACTATAGCTTTTT+AGG + chr2_4:17501497-17501516 None:intergenic 35.0%
AAGTAGGTTGTGTTAAGAGT+TGG + chr2_4:17501985-17502004 None:intergenic 35.0%
AGAACAATATGTCCCATTGA+TGG - chr2_4:17502015-17502034 Msa0318500:intron 35.0%
! AAATTGGTGAGTGTGCTATT+AGG + chr2_4:17502042-17502061 None:intergenic 35.0%
TAATAGCACACTCACCAATT+TGG - chr2_4:17502041-17502060 Msa0318500:intron 35.0%
CTCAAACCTAAGGATTCAAA+AGG - chr2_4:17502101-17502120 Msa0318500:intron 35.0%
! CAAGAATTGTTTTCCACAAG+CGG - chr2_4:17502141-17502160 Msa0318500:intron 35.0%
! GGAGTGTTAAATGAAACTCA+AGG - chr2_4:17502258-17502277 Msa0318500:CDS 35.0%
CAAGTGTCCTTGTAGAAGTT+AGG + chr2_4:17499881-17499900 None:intergenic 40.0%
GTATGCTTGCCAGTTTCATT+AGG - chr2_4:17499960-17499979 Msa0318500:CDS 40.0%
CACAACATCCAAACCTCTTA+TGG + chr2_4:17500355-17500374 None:intergenic 40.0%
GAATCAAATCAAAGACGCAG+TGG - chr2_4:17500374-17500393 Msa0318500:intron 40.0%
AGTAAAGAAAGCCTCTTAGC+CGG - chr2_4:17500840-17500859 Msa0318500:intron 40.0%
GAGAAACCTAGCATCAAAGA+TGG + chr2_4:17500866-17500885 None:intergenic 40.0%
TACTTGTGCCTCATATTTCC+TGG - chr2_4:17501273-17501292 Msa0318500:intron 40.0%
! TTCCTGGTGACATAATTTGC+AGG - chr2_4:17501289-17501308 Msa0318500:intron 40.0%
! TGACATAATTTGCAGGCTCT+TGG - chr2_4:17501296-17501315 Msa0318500:intron 40.0%
CTTGCTAATTCAAACCTTCC+TGG - chr2_4:17501374-17501393 Msa0318500:intron 40.0%
! GTTGAGACCTTGTTTAGCAA+AGG + chr2_4:17501432-17501451 None:intergenic 40.0%
ACTGATCTTGTTGCACTTTC+AGG - chr2_4:17501455-17501474 Msa0318500:intron 40.0%
!!! TGCTTATATTTTGGGACGGA+GGG + chr2_4:17501629-17501648 None:intergenic 40.0%
!!! TTGCTTATATTTTGGGACGG+AGG + chr2_4:17501628-17501647 None:intergenic 40.0%
!!! TTGCTTATATTTTGGGACGG+AGG - chr2_4:17501628-17501647 Msa0318500:intron 40.0%
!!! TGCTTATATTTTGGGACGGA+GGG - chr2_4:17501629-17501648 Msa0318500:intron 40.0%
ACAATTGGTAGAGGTCAATG+TGG - chr2_4:17501910-17501929 Msa0318500:intron 40.0%
GTTGTGTTAAGAGTTGGATC+GGG + chr2_4:17501979-17501998 None:intergenic 40.0%
GGTTGTGTTAAGAGTTGGAT+CGG + chr2_4:17501980-17501999 None:intergenic 40.0%
! GTGCTATTAGGTCCATCAAT+GGG + chr2_4:17502030-17502049 None:intergenic 40.0%
! TGTGCTATTAGGTCCATCAA+TGG + chr2_4:17502031-17502050 None:intergenic 40.0%
! ACAAGCCCTTTTGAATCCTT+AGG + chr2_4:17502110-17502129 None:intergenic 40.0%
CGCTTGTGGAAAACAATTCT+TGG + chr2_4:17502143-17502162 None:intergenic 40.0%
GAAACTATTGACAATGGCAG+TGG + chr2_4:17502175-17502194 None:intergenic 40.0%
ATGAAACTGGCAAGCATACG+AGG + chr2_4:17499959-17499978 None:intergenic 45.0%
TCCTAGTGTTGTGTCTTGTG+CGG - chr2_4:17500407-17500426 Msa0318500:intron 45.0%
TGTGCGGATATTCTTGCACT+TGG - chr2_4:17500423-17500442 Msa0318500:intron 45.0%
TGTGGAACATACCAGAACTG+TGG + chr2_4:17500460-17500479 None:intergenic 45.0%
AATGAGCAGGGTCAACCTAA+TGG + chr2_4:17500593-17500612 None:intergenic 45.0%
CTTGAATGAGCTGGCAAGTT+TGG + chr2_4:17500768-17500787 None:intergenic 45.0%
CAAACTTGCCAGCTCATTCA+AGG - chr2_4:17500766-17500785 Msa0318500:CDS 45.0%
TTTCTCGTCCTTGAATGAGC+TGG + chr2_4:17500777-17500796 None:intergenic 45.0%
AACCTAGCATCAAAGATGGC+CGG + chr2_4:17500862-17500881 None:intergenic 45.0%
AGCCTGCAAATTATGTCACC+AGG + chr2_4:17501294-17501313 None:intergenic 45.0%
CCTAATGGAACTTCCCAATC+AGG + chr2_4:17501323-17501342 None:intergenic 45.0%
CCTGATTGGGAAGTTCCATT+AGG - chr2_4:17501320-17501339 Msa0318500:intron 45.0%
GTAAGATCAAATTGGGGACC+AGG + chr2_4:17501395-17501414 None:intergenic 45.0%
! GAGACCTTGTTTAGCAAAGG+AGG + chr2_4:17501429-17501448 None:intergenic 45.0%
GGTGCTCATACAATTGGTAG+AGG - chr2_4:17501901-17501920 Msa0318500:intron 45.0%
! CGAATGTATCTGGAGTGGTT+GGG + chr2_4:17502068-17502087 None:intergenic 45.0%
! TCGAATGTATCTGGAGTGGT+TGG + chr2_4:17502069-17502088 None:intergenic 45.0%
TAGCTCGAGTCGAATGTATC+TGG + chr2_4:17502078-17502097 None:intergenic 45.0%
CGAGCTACTACTCAAACCTA+AGG - chr2_4:17502091-17502110 Msa0318500:intron 45.0%
ACCAAAGTCACCAAGGAAGA+TGG - chr2_4:17502327-17502346 Msa0318500:CDS 45.0%
! ACCATCTTCCTTGGTGACTT+TGG + chr2_4:17502331-17502350 None:intergenic 45.0%
GCTAACAGTACCATCTTCCT+TGG + chr2_4:17502340-17502359 None:intergenic 45.0%
!!! TCTTTTTATATTATTTTTTT+TGG + chr2_4:17500968-17500987 None:intergenic 5.0%
!! AAAAAAAAAAGTTTATTAAA+AGG + chr2_4:17501157-17501176 None:intergenic 5.0%
!! ATTATTATTATTAATTAAAG+TGG - chr2_4:17501735-17501754 Msa0318500:CDS 5.0%
!!! GTATTTTTTTAAAAAATATT+AGG - chr2_4:17501880-17501899 Msa0318500:intron 5.0%
TGCTGTGTAGTGGTTGTGCT+TGG - chr2_4:17499821-17499840 Msa0318500:CDS 50.0%
TGTGTAGTGGTTGTGCTTGG+AGG - chr2_4:17499824-17499843 Msa0318500:CDS 50.0%
GTGTAGTGGTTGTGCTTGGA+GGG - chr2_4:17499825-17499844 Msa0318500:CDS 50.0%
GATACTATCGTGAGCGAGCA+AGG - chr2_4:17500309-17500328 Msa0318500:intron 50.0%
TCCGCACAAGACACAACACT+AGG + chr2_4:17500411-17500430 None:intergenic 50.0%
ATCAAAGATGGCCGGCTAAG+AGG + chr2_4:17500854-17500873 None:intergenic 50.0%
GATCAAATTGGGGACCAGGA+AGG + chr2_4:17501391-17501410 None:intergenic 50.0%
TGGAGTGGTTGGGTCCAAAT+TGG + chr2_4:17502058-17502077 None:intergenic 50.0%
CGAGTCGAATGTATCTGGAG+TGG + chr2_4:17502073-17502092 None:intergenic 50.0%
! CCTTGGTGACTTTGGTAGCA+AGG + chr2_4:17502323-17502342 None:intergenic 50.0%
CCTTGCTACCAAAGTCACCA+AGG - chr2_4:17502320-17502339 Msa0318500:CDS 50.0%
! GGCAGCTTTGTGCTGTGTAG+TGG - chr2_4:17499811-17499830 Msa0318500:CDS 55.0%
AGCCGGCCATCTTTGATGCT+AGG - chr2_4:17500857-17500876 Msa0318500:intron 55.0%
TGCAGGCTCTTGGTCCTGAT+TGG - chr2_4:17501306-17501325 Msa0318500:intron 55.0%
GCAGGCTCTTGGTCCTGATT+GGG - chr2_4:17501307-17501326 Msa0318500:intron 55.0%
TCGTGAGCGAGCAAGGAGCT+AGG - chr2_4:17500316-17500335 Msa0318500:intron 60.0%
AGTGGTGTTTGCGCCGCTTG+TGG + chr2_4:17502157-17502176 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 17499785 17502368 17499785 ID=Msa0318500;Name=Msa0318500
chr2_4 mRNA 17499785 17502368 17499785 ID=Msa0318500-mRNA-1;Parent=Msa0318500;Name=Msa0318500-mRNA-1;_AED=0.35;_eAED=0.36;_QI=0|0.33|0.25|1|0.66|0.75|4|0|304
chr2_4 exon 17502225 17502368 17502225 ID=Msa0318500-mRNA-1:exon:6522;Parent=Msa0318500-mRNA-1
chr2_4 exon 17501686 17501877 17501686 ID=Msa0318500-mRNA-1:exon:6521;Parent=Msa0318500-mRNA-1
chr2_4 exon 17500710 17500842 17500710 ID=Msa0318500-mRNA-1:exon:6520;Parent=Msa0318500-mRNA-1
chr2_4 exon 17499785 17500230 17499785 ID=Msa0318500-mRNA-1:exon:6519;Parent=Msa0318500-mRNA-1
chr2_4 CDS 17502225 17502368 17502225 ID=Msa0318500-mRNA-1:cds;Parent=Msa0318500-mRNA-1
chr2_4 CDS 17501686 17501877 17501686 ID=Msa0318500-mRNA-1:cds;Parent=Msa0318500-mRNA-1
chr2_4 CDS 17500710 17500842 17500710 ID=Msa0318500-mRNA-1:cds;Parent=Msa0318500-mRNA-1
chr2_4 CDS 17499785 17500230 17499785 ID=Msa0318500-mRNA-1:cds;Parent=Msa0318500-mRNA-1
Gene Sequence

>Msa0318500

ATGAACTCTTCTTTTCATCTCATATTGGCAGCTTTGTGCTGTGTAGTGGTTGTGCTTGGAGGGTTTCCCTTCTCTTCAAATGCACAACTTGATCCTAACTTCTACAAGGACACTTGTCCAAATGTCACTTCCATTGTCAAACAAGGCTGTGATGCATCAATCTTGTTAAATGACACTGATACTATCGTGAGCGAGCAAGGAGCTAGGCCAAATAAAAACTCCATAAGAGGTTTGGATGTTGTGAATCAAATCAAAGACGCAGTGGAACTTGCTTGTCCTAGTGTTGTGTCTTGTGCGGATATTCTTGCACTTGGTGCTAATATTTCCACAGTTCTGGCTCTTGGTCCTGATTGGGAAGTTCCATTAGGAAGAAGAGATAGTTTGAATGCAAATCAAACTCTTGCTAATTCAAACCTTCCTGGTCCCCAATTTGATCTTACTCAGCTAATATCCTCCTTTGCTAAACAAGGTCAATGTGGATTGTTCTTTGATCGATTATACAATTTCAACGATACTAGAAAACCCGATCCAACTCTTAACACAACCTACTTACAAACATTGAGAACAATATGTCCCATTGATGGACCTAATAGCACACTCACCAATTTGGACCCAACCACTCCAGATACATTCGACTCGAGCTACTACTCAAACCTAAGGATTCAAAAGGGCTTGTTTCAGAGTGACCAAGAATTGTTTTCCACAAGCGGCGCAAACACCACTGCCATTGTCAATAGTTTCAACAACAACCAAACTCTTTTCTTTGAAGCTTTTAAGGCCTCTATGATTAAAATGGGTAATATTGGAGTGTTAAATGAAACTCAAGGTGAAGTTAGAACACACTGTAACTTTGTTAATACTGTTAGCCTTGCTACCAAAGTCACCAAGGAAGATGGTACTGTTAGCTCATTCTAA
Protein sequence

>Msa0318500

MNSSFHLILAALCCVVVVLGGFPFSSNAQLDPNFYKDTCPNVTSIVKQGCDASILLNDTDTIVSEQGARPNKNSIRGLDVVNQIKDAVELACPSVVSCADILALGANISTVLALGPDWEVPLGRRDSLNANQTLANSNLPGPQFDLTQLISSFAKQGQCGLFFDRLYNFNDTRKPDPTLNTTYLQTLRTICPIDGPNSTLTNLDPTTPDTFDSSYYSNLRIQKGLFQSDQELFSTSGANTTAIVNSFNNNQTLFFEAFKASMIKMGNIGVLNETQGEVRTHCNFVNTVSLATKVTKEDGTVSSF*