Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0329390 | AFK43929.1 | 100.000 | 70 | 0 | 0 | 1 | 70 | 1 | 70 | 1.60e-42 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0329390 | sp|Q9ASY8|TOM71_ARATH | 67.308 | 52 | 17 | 0 | 19 | 70 | 24 | 75 | 2.35e-22 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0329390 | Msa0546030 | 0.801947 | 7.080729e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0329390 | MtrunA17_Chr2g0311211 | 100.000 | 70 | 0 | 0 | 1 | 70 | 1 | 70 | 1.47e-46 | 142 |
| Msa0329390 | MtrunA17_Chr3g0103621 | 90.000 | 70 | 7 | 0 | 1 | 70 | 1 | 70 | 1.86e-41 | 130 |
| Msa0329390 | MtrunA17_Chr3g0100491 | 86.957 | 69 | 9 | 0 | 1 | 69 | 1 | 69 | 7.87e-40 | 125 |
| Msa0329390 | MtrunA17_Chr3g0103591 | 81.429 | 70 | 13 | 0 | 1 | 70 | 1 | 70 | 5.95e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0329390 | AT5G41685.1 | 67.308 | 52 | 17 | 0 | 19 | 70 | 24 | 75 | 2.39e-23 | 84.7 |
| Msa0329390 | AT1G64220.1 | 72.093 | 43 | 12 | 0 | 28 | 70 | 35 | 77 | 1.10e-18 | 73.2 |
Find 15 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGAGAAATTTGTGGTTT+TGG | 0.210299 | 2_4:-42744334 | None:intergenic |
| GACGAAACTCGAAATAGTTT+TGG | 0.299180 | 2_4:-42746784 | None:intergenic |
| ACAAAGGTTATTGCTCACTA+TGG | 0.348329 | 2_4:+42744271 | Msa0329390:CDS |
| ATTCCTTTGATTATCATCAT+TGG | 0.388372 | 2_4:+42744298 | Msa0329390:CDS |
| CTGAGAAGCTGAGAAATTTG+TGG | 0.449278 | 2_4:-42744340 | None:intergenic |
| CTGTGAAGGAATGGACAACA+TGG | 0.524007 | 2_4:+42744236 | Msa0329390:CDS |
| TGATCGATTCTGTGAAGGAA+TGG | 0.539433 | 2_4:+42744227 | Msa0329390:CDS |
| ATACCAATGATGATAATCAA+AGG | 0.596226 | 2_4:-42744301 | None:intergenic |
| ATCAGTGATCGATTCTGTGA+AGG | 0.598645 | 2_4:+42744222 | Msa0329390:CDS |
| TAGGGTTTCTCTGAAAGCGA+AGG | 0.599415 | 2_4:+42744168 | Msa0329390:CDS |
| AGGGTTTCTCTGAAAGCGAA+GGG | 0.627662 | 2_4:+42744169 | Msa0329390:CDS |
| TTCGCTTTCAGAGAAACCCT+AGG | 0.628634 | 2_4:-42744166 | None:intergenic |
| TGTGAAGGAATGGACAACAT+GGG | 0.649737 | 2_4:+42744237 | Msa0329390:CDS |
| ATGGGGTATGAAGAAAACAA+AGG | 0.679010 | 2_4:+42744255 | Msa0329390:CDS |
| GTGAAGGAATGGACAACATG+GGG | 0.691902 | 2_4:+42744238 | Msa0329390:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTGTTTAAAAAAATAAATT+AGG | + | chr2_4:42744576-42744595 | Msa0329390:intron | 10.0% |
| !! | AAAAAAATAAATTAGGATTT+AGG | + | chr2_4:42744583-42744602 | Msa0329390:intron | 10.0% |
| !!! | ATCTTATTTATGAAAAGTAT+TGG | + | chr2_4:42745497-42745516 | Msa0329390:intron | 15.0% |
| !!! | TCTTATTTATGAAAAGTATT+GGG | + | chr2_4:42745498-42745517 | Msa0329390:intron | 15.0% |
| !!! | TATGATGATATATTTCTATT+TGG | + | chr2_4:42745844-42745863 | Msa0329390:intron | 15.0% |
| !!! | ATAGTTTTTGATAGAAATAT+TGG | - | chr2_4:42746602-42746621 | None:intergenic | 15.0% |
| !!! | TTCTGTAGTTTTTTACTTTT+TGG | + | chr2_4:42744432-42744451 | Msa0329390:intron | 20.0% |
| !!! | TCTGTAGTTTTTTACTTTTT+GGG | + | chr2_4:42744433-42744452 | Msa0329390:intron | 20.0% |
| !! | AATAGATAATAGATGTATCT+AGG | + | chr2_4:42745332-42745351 | Msa0329390:intron | 20.0% |
| !!! | CTTATTTATGAAAAGTATTG+GGG | + | chr2_4:42745499-42745518 | Msa0329390:intron | 20.0% |
| !! | GTAAAATGAAAAGATATGAT+TGG | - | chr2_4:42745562-42745581 | None:intergenic | 20.0% |
| !!! | TTAAAGCTTAGTTTTAGTAT+TGG | + | chr2_4:42745690-42745709 | Msa0329390:intron | 20.0% |
| !!! | ATGTCTTTTCTTTGAATTAT+GGG | + | chr2_4:42745809-42745828 | Msa0329390:intron | 20.0% |
| !! | AAGAATACATATGTATTGAA+TGG | - | chr2_4:42746045-42746064 | None:intergenic | 20.0% |
| !!! | CTATTTTATCAAATTATGAC+TGG | + | chr2_4:42746208-42746227 | Msa0329390:intron | 20.0% |
| !!! | TTTACATAGTCTAATAGTTT+TGG | - | chr2_4:42746572-42746591 | None:intergenic | 20.0% |
| !! | TATTAGACTATGTAAATACA+CGG | + | chr2_4:42746576-42746595 | Msa0329390:intron | 20.0% |
| ! | ATTCCTTTGATTATCATCAT+TGG | + | chr2_4:42744298-42744317 | Msa0329390:CDS | 25.0% |
| ! | ATACCAATGATGATAATCAA+AGG | - | chr2_4:42744304-42744323 | None:intergenic | 25.0% |
| ! | ACAAGAAACTTTAACATCAT+TGG | - | chr2_4:42744561-42744580 | None:intergenic | 25.0% |
| ! | GGATAAATTTGGATTAACTA+CGG | + | chr2_4:42744860-42744879 | Msa0329390:intron | 25.0% |
| ! | ATCAATTTATGTGCATCAAT+CGG | + | chr2_4:42744969-42744988 | Msa0329390:intron | 25.0% |
| ! | TCATTACACATAAAAATCTG+AGG | - | chr2_4:42745144-42745163 | None:intergenic | 25.0% |
| ! | ATTTAGCTGAAGAATTATCA+TGG | + | chr2_4:42745171-42745190 | Msa0329390:intron | 25.0% |
| ! | ATGCATTAAGTGATATATCT+AGG | + | chr2_4:42745279-42745298 | Msa0329390:intron | 25.0% |
| ! | TGCATTAAGTGATATATCTA+GGG | + | chr2_4:42745280-42745299 | Msa0329390:intron | 25.0% |
| ! | AAGAAGGAAAAAAATAACGT+TGG | - | chr2_4:42745535-42745554 | None:intergenic | 25.0% |
| ! | ACATTATTCTGTTGTGTATA+TGG | + | chr2_4:42745580-42745599 | Msa0329390:intron | 25.0% |
| !!! | TTTAGTATTGGATTTCTAGT+TGG | + | chr2_4:42745702-42745721 | Msa0329390:intron | 25.0% |
| !! | GTATGTCTTCAAAATTTTAG+AGG | + | chr2_4:42745727-42745746 | Msa0329390:intron | 25.0% |
| !! | CATGTCTTTTCTTTGAATTA+TGG | + | chr2_4:42745808-42745827 | Msa0329390:intron | 25.0% |
| ! | GAAATATATCATCATATGCA+TGG | - | chr2_4:42745840-42745859 | None:intergenic | 25.0% |
| !! | GTATGTCTTCAAAATTTTAG+AGG | + | chr2_4:42745727-42745746 | Msa0329390:intron | 25.0% |
| !! | AAAATTTGAGTTCAAAGCAT+TGG | - | chr2_4:42746016-42746035 | None:intergenic | 25.0% |
| !! | CATATGGTTTGATTTAAATC+AGG | - | chr2_4:42746149-42746168 | None:intergenic | 25.0% |
| ! | AAGAGAATGATTCTTAGATA+TGG | - | chr2_4:42746344-42746363 | None:intergenic | 25.0% |
| !!! | TCTATAATTAAGCGTTGATT+AGG | + | chr2_4:42746365-42746384 | Msa0329390:intron | 25.0% |
| !!! | CTATAATTAAGCGTTGATTA+GGG | + | chr2_4:42746366-42746385 | Msa0329390:intron | 25.0% |
| ! | TATATTAAACTTGGATTCCA+AGG | - | chr2_4:42746418-42746437 | None:intergenic | 25.0% |
| !! | ATTTTCACTTGCTTTTCTGA+TGG | + | chr2_4:42744385-42744404 | Msa0329390:intron | 30.0% |
| ! | GCTTTTCTGATGGAATATTA+TGG | + | chr2_4:42744395-42744414 | Msa0329390:intron | 30.0% |
| GGAATATTATGGAATCAATG+TGG | + | chr2_4:42744406-42744425 | Msa0329390:intron | 30.0% | |
| !! | TACTTGATGATGTTTATGCT+TGG | + | chr2_4:42744512-42744531 | Msa0329390:intron | 30.0% |
| !! | ATCAAATTTTTCAGAACCAG+CGG | + | chr2_4:42744680-42744699 | Msa0329390:intron | 30.0% |
| CATAGGAACAAAACTAATGA+AGG | - | chr2_4:42744787-42744806 | None:intergenic | 30.0% | |
| TGTACAAATAAGAGTAGCAT+AGG | - | chr2_4:42744804-42744823 | None:intergenic | 30.0% | |
| TAAAATCAACACTACTGAGA+AGG | - | chr2_4:42744905-42744924 | None:intergenic | 30.0% | |
| GAGTAATGACAAACAAAATC+AGG | - | chr2_4:42745455-42745474 | None:intergenic | 30.0% | |
| AGATATGATTGGAAAGAAGA+AGG | - | chr2_4:42745551-42745570 | None:intergenic | 30.0% | |
| GAGATATCATCATATGCTAT+AGG | - | chr2_4:42745667-42745686 | None:intergenic | 30.0% | |
| TAGGCATGTCTTTGAATTAT+GGG | + | chr2_4:42745940-42745959 | Msa0329390:intron | 30.0% | |
| ! | AACCATATGCTACATTTTGA+AGG | + | chr2_4:42746160-42746179 | Msa0329390:intron | 30.0% |
| GAAAATACACTTTGATACCT+TGG | + | chr2_4:42746398-42746417 | Msa0329390:intron | 30.0% | |
| CAGTTCTGCTATATTAAACT+TGG | - | chr2_4:42746427-42746446 | None:intergenic | 30.0% | |
| ! | TGCGTTAAATGTTTTGTCTA+TGG | - | chr2_4:42746531-42746550 | None:intergenic | 30.0% |
| !!! | AGTTTTGTTCACTCATATGA+TGG | - | chr2_4:42746729-42746748 | None:intergenic | 30.0% |
| ATGGGGTATGAAGAAAACAA+AGG | + | chr2_4:42744255-42744274 | Msa0329390:CDS | 35.0% | |
| ACAAAGGTTATTGCTCACTA+TGG | + | chr2_4:42744271-42744290 | Msa0329390:CDS | 35.0% | |
| ! | AGCTGAGAAATTTGTGGTTT+TGG | - | chr2_4:42744337-42744356 | None:intergenic | 35.0% |
| ! | AATGTGCAACATCGATTACA+CGG | - | chr2_4:42744368-42744387 | None:intergenic | 35.0% |
| AATAATTCCGGGTTATGGAT+AGG | + | chr2_4:42744703-42744722 | Msa0329390:intron | 35.0% | |
| GCGAAATGACATTGACATTA+TGG | + | chr2_4:42745047-42745066 | Msa0329390:intron | 35.0% | |
| GAGGATCAAGTTCATAAGAA+AGG | - | chr2_4:42745125-42745144 | None:intergenic | 35.0% | |
| !!! | ATGTTTTAGAGCTTGTGTCA+TGG | + | chr2_4:42745370-42745389 | Msa0329390:intron | 35.0% |
| TGTACCTACCGTAGTATATA+CGG | - | chr2_4:42745420-42745439 | None:intergenic | 35.0% | |
| AGTGCTATATTTAGTGTGAC+TGG | + | chr2_4:42745605-42745624 | Msa0329390:intron | 35.0% | |
| ! | GTAGTTTTTCCAAACCAGAA+TGG | + | chr2_4:42745759-42745778 | Msa0329390:intron | 35.0% |
| ACTAACAAGTAACACCATTC+TGG | - | chr2_4:42745776-42745795 | None:intergenic | 35.0% | |
| ! | TACTTGTTAGTCCTCATACA+AGG | + | chr2_4:42745785-42745804 | Msa0329390:intron | 35.0% |
| TAGTTCTTTCCAAACCAGAA+TGG | + | chr2_4:42745902-42745921 | Msa0329390:intron | 35.0% | |
| TCAAAGACATGCCTAGTATA+AGG | - | chr2_4:42745935-42745954 | None:intergenic | 35.0% | |
| CTAGGCATGTCTTTGAATTA+TGG | + | chr2_4:42745939-42745958 | Msa0329390:intron | 35.0% | |
| !!! | TTGTTTTGTTTGACCGTACA+CGG | + | chr2_4:42745988-42746007 | Msa0329390:intron | 35.0% |
| CACCTTCAAAATGTAGCATA+TGG | - | chr2_4:42746165-42746184 | None:intergenic | 35.0% | |
| ATAGAAGTGACTGTATATGC+TGG | - | chr2_4:42746192-42746211 | None:intergenic | 35.0% | |
| TTCACTCATATGATGGTATC+TGG | - | chr2_4:42746722-42746741 | None:intergenic | 35.0% | |
| ! | GACGAAACTCGAAATAGTTT+TGG | - | chr2_4:42746787-42746806 | None:intergenic | 35.0% |
| AAAAAGAAGAATGGCGCCTA+GGG | + | chr2_4:42744150-42744169 | Msa0329390:exon | 40.0% | |
| ! | ATCAGTGATCGATTCTGTGA+AGG | + | chr2_4:42744222-42744241 | Msa0329390:CDS | 40.0% |
| ! | TGATCGATTCTGTGAAGGAA+TGG | + | chr2_4:42744227-42744246 | Msa0329390:CDS | 40.0% |
| TGTGAAGGAATGGACAACAT+GGG | + | chr2_4:42744237-42744256 | Msa0329390:CDS | 40.0% | |
| CTGAGAAGCTGAGAAATTTG+TGG | - | chr2_4:42744343-42744362 | None:intergenic | 40.0% | |
| ! | ATGTGCAACATCGATTACAC+GGG | - | chr2_4:42744367-42744386 | None:intergenic | 40.0% |
| ATGTTTATGCTTGGTTGAGC+AGG | + | chr2_4:42744521-42744540 | Msa0329390:intron | 40.0% | |
| CATTGTTCCTATCCATAACC+CGG | - | chr2_4:42744713-42744732 | None:intergenic | 40.0% | |
| CTCTCTCCATTTCCACATTT+CGG | + | chr2_4:42744747-42744766 | Msa0329390:intron | 40.0% | |
| TAAAACCAAGTGTGACAGAG+AGG | + | chr2_4:42744838-42744857 | Msa0329390:intron | 40.0% | |
| AAAACCAAGTGTGACAGAGA+GGG | + | chr2_4:42744839-42744858 | Msa0329390:intron | 40.0% | |
| GTGACAGAGAGGGATAAATT+TGG | + | chr2_4:42744849-42744868 | Msa0329390:intron | 40.0% | |
| ! | CGGTTACCATGGTAATGAAA+TGG | + | chr2_4:42744989-42745008 | Msa0329390:intron | 40.0% |
| CAGTGTCCATTTCATTACCA+TGG | - | chr2_4:42744998-42745017 | None:intergenic | 40.0% | |
| CTGGTAACCCGTATATACTA+CGG | + | chr2_4:42745409-42745428 | Msa0329390:intron | 40.0% | |
| TAACCCGTATATACTACGGT+AGG | + | chr2_4:42745413-42745432 | Msa0329390:intron | 40.0% | |
| GTACCTACCGTAGTATATAC+GGG | - | chr2_4:42745419-42745438 | None:intergenic | 40.0% | |
| ! | CAAGTAACACCATTCTGGTT+TGG | - | chr2_4:42745771-42745790 | None:intergenic | 40.0% |
| GAAAAGACATGCCTTGTATG+AGG | - | chr2_4:42745799-42745818 | None:intergenic | 40.0% | |
| TATAAGGACAGACACCATTC+TGG | - | chr2_4:42745919-42745938 | None:intergenic | 40.0% | |
| ATGGTGTCTGTCCTTATACT+AGG | + | chr2_4:42745921-42745940 | Msa0329390:intron | 40.0% | |
| !! | TTGGTTTGTCTGCTTTGCAT+CGG | - | chr2_4:42746070-42746089 | None:intergenic | 40.0% |
| GCAGAACTGAACACTTTGAT+TGG | + | chr2_4:42746438-42746457 | Msa0329390:intron | 40.0% | |
| ! | ATGTTTTGTCTATGGCTGCA+AGG | - | chr2_4:42746523-42746542 | None:intergenic | 40.0% |
| TTCGCTTTCAGAGAAACCCT+AGG | - | chr2_4:42744169-42744188 | None:intergenic | 45.0% | |
| ! | TAGGGTTTCTCTGAAAGCGA+AGG | + | chr2_4:42744168-42744187 | Msa0329390:CDS | 45.0% |
| ! | AGGGTTTCTCTGAAAGCGAA+GGG | + | chr2_4:42744169-42744188 | Msa0329390:CDS | 45.0% |
| CTGTGAAGGAATGGACAACA+TGG | + | chr2_4:42744236-42744255 | Msa0329390:CDS | 45.0% | |
| GTGAAGGAATGGACAACATG+GGG | + | chr2_4:42744238-42744257 | Msa0329390:CDS | 45.0% | |
| ! | TGTGCAACATCGATTACACG+GGG | - | chr2_4:42744366-42744385 | None:intergenic | 45.0% |
| CAGAACCAGCGGAATAATTC+CGG | + | chr2_4:42744691-42744710 | Msa0329390:intron | 45.0% | |
| AGAACCAGCGGAATAATTCC+GGG | + | chr2_4:42744692-42744711 | Msa0329390:intron | 45.0% | |
| ATAACCCGGAATTATTCCGC+TGG | - | chr2_4:42744699-42744718 | None:intergenic | 45.0% | |
| AGCGGAATAATTCCGGGTTA+TGG | + | chr2_4:42744698-42744717 | Msa0329390:intron | 45.0% | |
| CGAAATGTGGAAATGGAGAG+AGG | - | chr2_4:42744749-42744768 | None:intergenic | 45.0% | |
| CCTCATCCGAAATGTGGAAA+TGG | - | chr2_4:42744756-42744775 | None:intergenic | 45.0% | |
| CCATTTCCACATTTCGGATG+AGG | + | chr2_4:42744753-42744772 | Msa0329390:intron | 45.0% | |
| ATTCAGCCTCATCCGAAATG+TGG | - | chr2_4:42744762-42744781 | None:intergenic | 45.0% | |
| TTATCCCTCTCTGTCACACT+TGG | - | chr2_4:42744846-42744865 | None:intergenic | 45.0% | |
| ATGTGCACACATCGATCACA+AGG | - | chr2_4:42744932-42744951 | None:intergenic | 45.0% | |
| TGTGCATCAATCGGTTACCA+TGG | + | chr2_4:42744978-42744997 | Msa0329390:intron | 45.0% | |
| TTAGAGCTTGTGTCATGGAC+TGG | + | chr2_4:42745375-42745394 | Msa0329390:intron | 45.0% | |
| !! | TGGACTGGTGGTATATGATC+TGG | + | chr2_4:42745390-42745409 | Msa0329390:intron | 45.0% |
| !! | CAAAGCATTGGTTCCGTGTA+CGG | - | chr2_4:42746004-42746023 | None:intergenic | 45.0% |
| !! | CTGAGACAACTAAGAGCACT+TGG | - | chr2_4:42746089-42746108 | None:intergenic | 45.0% |
| !! | TTCTATAAAAAAATATTTAT+TGG | + | chr2_4:42746495-42746514 | Msa0329390:intron | 5.0% |
| ! | GGACAGACACCATTCTGGTT+TGG | - | chr2_4:42745914-42745933 | None:intergenic | 50.0% |
| !! | GAGCTTGTGTCATGGACTGG+TGG | + | chr2_4:42745378-42745397 | Msa0329390:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 42744150 | 42746812 | 42744150 | ID=Msa0329390;Name=Msa0329390 |
| chr2_4 | mRNA | 42744150 | 42746812 | 42744150 | ID=Msa0329390-mRNA-1;Parent=Msa0329390;Name=Msa0329390-mRNA-1;_AED=0.47;_eAED=0.47;_QI=10|0|0|0.5|0|0.5|2|0|85 |
| chr2_4 | exon | 42744150 | 42744368 | 42744150 | ID=Msa0329390-mRNA-1:exon:13159;Parent=Msa0329390-mRNA-1 |
| chr2_4 | exon | 42746767 | 42746812 | 42746767 | ID=Msa0329390-mRNA-1:exon:13160;Parent=Msa0329390-mRNA-1 |
| chr2_4 | five_prime_UTR | 42744150 | 42744159 | 42744150 | ID=Msa0329390-mRNA-1:five_prime_utr;Parent=Msa0329390-mRNA-1 |
| chr2_4 | CDS | 42744160 | 42744368 | 42744160 | ID=Msa0329390-mRNA-1:cds;Parent=Msa0329390-mRNA-1 |
| chr2_4 | CDS | 42746767 | 42746812 | 42746767 | ID=Msa0329390-mRNA-1:cds;Parent=Msa0329390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
