Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0331090 | Msa0331550 | 0.825573 | 4.354286e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTACTGCCATATTGTTGC+AGG | 0.267857 | 2_4:+47497502 | Msa0331550:CDS |
| GACAAAGCTTCCTTTCATAA+TGG | 0.348216 | 2_4:+47497467 | Msa0331550:CDS |
| CAATATGGCAGTAGCAGAAA+TGG | 0.389651 | 2_4:-47497495 | None:intergenic |
| GATCCTTACTCACTTGATAT+AGG | 0.403110 | 2_4:+47497963 | Msa0331550:CDS |
| CTTCCTATATCAAGTGAGTA+AGG | 0.454009 | 2_4:-47497966 | None:intergenic |
| AAGCTTCCTTTCATAATGGA+AGG | 0.546475 | 2_4:+47497471 | Msa0331550:CDS |
| AATGGACCTTCCATTATGAA+AGG | 0.578687 | 2_4:-47497477 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTAACATTCTATTTTTCTT+AGG | - | chr2_4:47497891-47497910 | None:intergenic | 20.0% |
| ! | CTACACAAATAAAACTTTGA+CGG | - | chr2_4:47497687-47497706 | None:intergenic | 25.0% |
| ! | TTTATTTGTGTAGAAGTTAC+TGG | + | chr2_4:47497694-47497713 | Msa0331550:intron | 25.0% |
| ! | TGACTATCCAAAAAAATTGA+TGG | - | chr2_4:47497767-47497786 | None:intergenic | 25.0% |
| ! | TTTCTTAGGTAAATATTGTG+TGG | - | chr2_4:47497877-47497896 | None:intergenic | 25.0% |
| !! | TCTGTTATTCTTTTTAATGC+AGG | + | chr2_4:47497941-47497960 | Msa0331550:intron | 25.0% |
| AAAAATCACCTGCAACAATA+TGG | - | chr2_4:47497513-47497532 | None:intergenic | 30.0% | |
| AGGTAAACTACCATCAAATT+TGG | + | chr2_4:47497546-47497565 | Msa0331550:intron | 30.0% | |
| AAACTACCATCAAATTTGGT+TGG | + | chr2_4:47497550-47497569 | Msa0331550:intron | 30.0% | |
| ATCAGAAATTCGTAGTCATA+GGG | - | chr2_4:47497621-47497640 | None:intergenic | 30.0% | |
| TATCAGAAATTCGTAGTCAT+AGG | - | chr2_4:47497622-47497641 | None:intergenic | 30.0% | |
| !!! | TGTTAGTTTTGTGCAAGAAT+TGG | + | chr2_4:47497652-47497671 | Msa0331550:intron | 30.0% |
| ! | TATCCAAAAAAATTGATGGC+AGG | - | chr2_4:47497763-47497782 | None:intergenic | 30.0% |
| !! | AGTTTAAAATCGTTGATAGC+AGG | - | chr2_4:47497798-47497817 | None:intergenic | 30.0% |
| ! | GATATAGGAAGATTTTTCAC+AGG | + | chr2_4:47497978-47497997 | Msa0331550:CDS | 30.0% |
| GACAAAGCTTCCTTTCATAA+TGG | + | chr2_4:47497467-47497486 | Msa0331550:CDS | 35.0% | |
| ! | AAGCTTCCTTTCATAATGGA+AGG | + | chr2_4:47497471-47497490 | Msa0331550:CDS | 35.0% |
| AATGGACCTTCCATTATGAA+AGG | - | chr2_4:47497480-47497499 | None:intergenic | 35.0% | |
| ! | TTGTTGCAGGTGATTTTTGT+TGG | + | chr2_4:47497515-47497534 | Msa0331550:CDS | 35.0% |
| GATGTTCCAACCAAATTTGA+TGG | - | chr2_4:47497559-47497578 | None:intergenic | 35.0% | |
| AATTTGGTTGGAACATCTGT+TGG | + | chr2_4:47497562-47497581 | Msa0331550:intron | 35.0% | |
| TTTGTGTAGAAGTTACTGGT+AGG | + | chr2_4:47497698-47497717 | Msa0331550:intron | 35.0% | |
| ! | AAAAAATTGATGGCAGGATG+AGG | - | chr2_4:47497757-47497776 | None:intergenic | 35.0% |
| !!! | CATCCTGCCATCAATTTTTT+TGG | + | chr2_4:47497757-47497776 | Msa0331550:intron | 35.0% |
| TCTTAATCTCACACACTTCA+AGG | + | chr2_4:47497841-47497860 | Msa0331550:intron | 35.0% | |
| GATCCTTACTCACTTGATAT+AGG | + | chr2_4:47497963-47497982 | Msa0331550:CDS | 35.0% | |
| CTTCCTATATCAAGTGAGTA+AGG | - | chr2_4:47497969-47497988 | None:intergenic | 35.0% | |
| ! | GGAAGATTTTTCACAGGATA+TGG | + | chr2_4:47497984-47498003 | Msa0331550:CDS | 35.0% |
| CAATATGGCAGTAGCAGAAA+TGG | - | chr2_4:47497498-47497517 | None:intergenic | 40.0% | |
| TGAAGCAGCCATTGAATCAT+AGG | + | chr2_4:47497593-47497612 | Msa0331550:intron | 40.0% | |
| ! | ATAGGGCACCTATGATTCAA+TGG | - | chr2_4:47497604-47497623 | None:intergenic | 40.0% |
| TCATACACCTAGAGTCATTG+TGG | + | chr2_4:47497728-47497747 | Msa0331550:intron | 40.0% | |
| CATACACCTAGAGTCATTGT+GGG | + | chr2_4:47497729-47497748 | Msa0331550:intron | 40.0% | |
| TGCTACTGCCATATTGTTGC+AGG | + | chr2_4:47497502-47497521 | Msa0331550:CDS | 45.0% | |
| ! | GCAGGTGATTTTTGTTGGCA+CGG | + | chr2_4:47497520-47497539 | Msa0331550:CDS | 45.0% |
| ! | CAGGTGATTTTTGTTGGCAC+GGG | + | chr2_4:47497521-47497540 | Msa0331550:CDS | 45.0% |
| ! | GATTTTTGTTGGCACGGGTA+AGG | + | chr2_4:47497526-47497545 | Msa0331550:CDS | 45.0% |
| GAGGATCCCACAATGACTCT+AGG | - | chr2_4:47497738-47497757 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 47497458 | 47498013 | 47497458 | ID=Msa0331550;Name=Msa0331550 |
| chr2_4 | mRNA | 47497458 | 47498013 | 47497458 | ID=Msa0331550-mRNA-1;Parent=Msa0331550;Name=Msa0331550-mRNA-1;_AED=0.43;_eAED=0.83;_QI=0|0|0|1|1|0.5|2|0|46 |
| chr2_4 | exon | 47497458 | 47497547 | 47497458 | ID=Msa0331550-mRNA-1:exon:14294;Parent=Msa0331550-mRNA-1 |
| chr2_4 | exon | 47497963 | 47498013 | 47497963 | ID=Msa0331550-mRNA-1:exon:14295;Parent=Msa0331550-mRNA-1 |
| chr2_4 | CDS | 47497458 | 47497547 | 47497458 | ID=Msa0331550-mRNA-1:cds;Parent=Msa0331550-mRNA-1 |
| chr2_4 | CDS | 47497963 | 47498013 | 47497963 | ID=Msa0331550-mRNA-1:cds;Parent=Msa0331550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
