AlfalfaGEDB | Msa0336950

M. sativa cultivar zhongmu-4 / Msa0336950

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF08276.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336950	XP_003597080.1	86.713	429	55	1	1	429	366	792	0.0	735

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336950	sp\|O64477\|Y2913_ARATH	58.756	434	169	6	1	427	355	785	2.75e-166	491

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0336920	Msa0336950	0.974874	6.238215e-139	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336950	MtrunA17_Chr2g0323341	86.713	429	55	1	1	429	274	700	0.0	734
Msa0336950	MtrunA17_Chr2g0323301	72.326	430	113	3	1	429	363	787	0.0	613
Msa0336950	MtrunA17_Chr4g0023071	65.581	430	139	4	1	429	364	785	0.0	582
Msa0336950	MtrunA17_Chr8g0348661	45.035	433	209	11	18	427	356	782	6.69e-112	347
Msa0336950	MtrunA17_Chr1g0202331	43.023	430	213	10	16	421	398	819	2.73e-105	331
Msa0336950	MtrunA17_Chr2g0314611	42.233	412	220	7	16	418	364	766	9.35e-105	328
Msa0336950	MtrunA17_Chr2g0318171	41.889	413	220	8	16	418	360	762	1.62e-104	327
Msa0336950	MtrunA17_Chr3g0137741	40.647	433	234	8	6	422	351	776	2.67e-104	327
Msa0336950	MtrunA17_Chr6g0467281	50.751	333	150	8	101	422	446	775	1.24e-102	323
Msa0336950	MtrunA17_Chr1g0149381	40.952	420	225	6	18	420	368	781	1.18e-100	318

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336950	AT2G19130.1	58.756	434	169	6	1	427	355	785	2.80e-167	491
Msa0336950	AT5G24080.3	44.626	428	213	9	12	421	267	688	5.98e-112	345
Msa0336950	AT5G24080.1	44.626	428	213	9	12	421	267	688	5.98e-112	345
Msa0336950	AT5G24080.2	44.626	428	213	9	12	421	394	815	6.59e-111	346
Msa0336950	AT4G00340.1	46.359	412	197	10	16	410	357	761	1.67e-106	333
Msa0336950	AT1G34300.1	43.056	432	218	11	12	421	344	769	2.29e-104	328
Msa0336950	AT4G32300.1	41.531	431	228	9	4	418	352	774	7.11e-104	327
Msa0336950	AT4G00340.2	45.742	411	180	10	16	410	357	740	4.40e-103	324
Msa0336950	AT5G35370.1	37.199	457	230	12	18	425	372	820	1.18e-85	280
Msa0336950	AT5G20050.1	40.274	365	174	9	92	418	40	398	6.97e-80	254

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Find 98 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATACTTTCTTTCCAACCT+TGG	0.447763	2_4:-56526661	Msa0336950:CDS
AAATCTGATGTTTACAGCTA+TGG	0.466655	2_4:-56526744	Msa0336950:CDS
AAATGATAAAAGTTGCTTCT+TGG	0.311867	2_4:-56526551	Msa0336950:CDS
AAATTGCTCTTGGAATTGCA+AGG	0.573740	2_4:-56526983	Msa0336950:CDS
AAATTGGGAGGAGGAGGAGG+AGG	0.372059	2_4:-56527275	Msa0336950:CDS
AACCTTCTGAGCAGAATCCT+CGG	0.534003	2_4:+56527108	None:intergenic
AACGTTAGCTGATTCAAGTG+TGG	0.668187	2_4:-56527231	Msa0336950:CDS
AAGAAAGAGAATGATTGCAA+TGG	0.565041	2_4:-56527372	Msa0336950:CDS
AAGTCCTAGATAACCACTCT+GGG	0.534180	2_4:+56526778	None:intergenic
AAGTTGGAGGGTGTTAGCCA+AGG	0.599157	2_4:-56527197	Msa0336950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCTTATGCATATGATAGTAA+TGG	-	chr2_4:56526515-56526534	Msa0336950:CDS	30.0%
ACAATTGTTGCAGATTAATG+AGG	+	chr2_4:56526562-56526581	None:intergenic	30.0%
TGCTAGTAAAAACAGTATTC+AGG	-	chr2_4:56526643-56526662	Msa0336950:CDS	30.0%
TAAAAACAGTATTCAGGTGA+GGG	-	chr2_4:56526649-56526668	Msa0336950:CDS	30.0%
AAGAAAGAGAATGATTGCAA+TGG	-	chr2_4:56526751-56526770	Msa0336950:CDS	30.0%
AGAAAGAGAATGATTGCAAT+GGG	-	chr2_4:56526752-56526771	Msa0336950:CDS	30.0%
CAAAAATTTCTCCGAGAAAT+TGG	-	chr2_4:56526832-56526851	Msa0336950:CDS	30.0%
AGTTATTGTTCCTGAACAAA+TGG	+	chr2_4:56527090-56527109	None:intergenic	30.0%
AGAATCGTTTCAAATATGAG+AGG	-	chr2_4:56527304-56527323	Msa0336950:CDS	30.0%
ATAGCTGTAAACATCAGATT+TGG	+	chr2_4:56527380-56527399	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	56526428	56527717	56526428	ID=Msa0336950;Name=Msa0336950
chr2_4	mRNA	56526428	56527717	56526428	ID=Msa0336950-mRNA-1;Parent=Msa0336950;Name=Msa0336950-mRNA-1;_AED=0.18;_eAED=0.19;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|429
chr2_4	exon	56526428	56527717	56526428	ID=Msa0336950-mRNA-1:exon:17267;Parent=Msa0336950-mRNA-1
chr2_4	CDS	56526428	56527717	56526428	ID=Msa0336950-mRNA-1:cds;Parent=Msa0336950-mRNA-1

Gene Sequence

>Msa0336950

ATGGCTTTACCTAAATTTTCCCAATTTGTGGGGTTAGGGAATGCAGCTGAATGTGAATTAACTTGCTTGAAAAACTGTTCATGCACTGCTTATGCATATGATAGTAATGGATGTTCAATTTGGGTAGGAGACCTCATTAATCTGCAACAATTGTCTTCTGATAATAGTAATCGAAAAACTTTATATGTCAAGCTTGCAGCATTAGAATTACGTGATGCTAGTAAAAACAGTATTCAGGTGAGGGTGATTATTGGTGGTGTTGTGGGCGGAGTTGTTGGCATAGGGATACTCTTGGTCCTTCTATTGTTTATCATGCTTAGGCGAAGAAAGAGAATGATTGCAATGGGAAAGCTCTTGGAGGGTTTTATAGTGGAATTTGGGTATAGGGATTTGCAAAATGCAACCAAAAATTTCTCCGAGAAATTGGGAGGAGGAGGAGGAGGTTTTGGTTCTATTTTCAAAGGAACGTTAGCTGATTCAAGTGTGGTGGCAGTGAAGAAGTTGGAGGGTGTTAGCCAAGGAGAGAAGCAATTCAGAACAAAAGTGAGTATAATAGGGACAACACAACATGTTAATCTTATTCGCCTCCGAGGATTCTGCTCAGAAGGTTCCAAAAAGTTGCTGGTTTATGATTACATGCCAAATTGTTCCTTGGATCTCCATTTGTTCAGGAACAATAACTCTGCGGTGTTACGCTGGAGTATCAGATACCAAATTGCTCTTGGAATTGCAAGGGGACTAATTTACCTACACGAGAAATGCGAAGAATGTATCATACACTATGATATAAAGCCAGAAAACATTCTCCTTGATGCTGATTTTTGTCCAAAAGTTGCGGATTTTGGCCTAGCTAAGCTAATTGGACGGGATTTCAGCAGAATCGTTTCAAATATGAGAGGAACGAGAAGCTATCTTGCCCCAGAGTGGTTATCTAGGACTTCTATCACAGCCAAATCTGATGTTTACAGCTATGGAATGATGCTTTTTGAGGTTGTATCAGGTAAAAGGAATTCTGATCCATCTGCAAATGACCAAAATACTTTCTTTCCAACCTTGGCTGCTACAGTAGTTAACCAAGGTGGTAGTATCCTTACCCTTTTGGACCCTAGGTTGGAGGGAAATGCTGACATTGAGGAAGTGACTGAAATGATAAAAGTTGCTTCTTGGTGTGTCCAAGAAAATGAAACTCAGAGGCCAACCATGCGTCAGGCAGTTCAAATTCTCGAGGGGACCTTGAATGTGAATTTGCCACCAATTCCAAAATTCAATCAACTGTTTGTAGATAACTAA

Protein sequence

>Msa0336950

MALPKFSQFVGLGNAAECELTCLKNCSCTAYAYDSNGCSIWVGDLINLQQLSSDNSNRKTLYVKLAALELRDASKNSIQVRVIIGGVVGGVVGIGILLVLLLFIMLRRRKRMIAMGKLLEGFIVEFGYRDLQNATKNFSEKLGGGGGGFGSIFKGTLADSSVVAVKKLEGVSQGEKQFRTKVSIIGTTQHVNLIRLRGFCSEGSKKLLVYDYMPNCSLDLHLFRNNNSAVLRWSIRYQIALGIARGLIYLHEKCEECIIHYDIKPENILLDADFCPKVADFGLAKLIGRDFSRIVSNMRGTRSYLAPEWLSRTSITAKSDVYSYGMMLFEVVSGKRNSDPSANDQNTFFPTLAATVVNQGGSILTLLDPRLEGNADIEEVTEMIKVASWCVQENETQRPTMRQAVQILEGTLNVNLPPIPKFNQLFVDN*