AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0345990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0345990 MtrunA17_Chr3g0078381 100.000 147 0 0 10 156 1 147 3.59e-105 296
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0345990 AT3G22480.1 70.667 150 39 3 10 156 1 148 2.24e-67 201
Msa0345990 AT3G22480.2 70.667 150 39 3 10 156 1 148 2.24e-67 201

Find 59 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAACAAAATGCTAAATT+TGG 0.143092 3_1:+5835102 Msa0345990:three_prime_UTR
CATTTCAAGTTCCGTTATTT+TGG 0.192430 3_1:-5834482 None:intergenic
GCAACTGCTTGTTCATTTAC+TGG 0.248625 3_1:-5834415 None:intergenic
AATATAAGCATTGCAACTTA+TGG 0.272338 3_1:-5835202 None:intergenic
TACTGGCTCCTTGCGATCTT+CGG 0.331035 3_1:-5834398 None:intergenic
CAAAATAACGGAACTTGAAA+TGG 0.346568 3_1:+5834483 Msa0345990:CDS
AGTTTATTGAACTAAATGTT+AGG 0.375281 3_1:+5830521 None:intergenic
TGAAGTAAACTTATCTTCAA+TGG 0.407937 3_1:+5834974 Msa0345990:three_prime_UTR
TGTAAATTTGTGTAGAATCA+TGG 0.408527 3_1:+5834366 Msa0345990:intron
AGCAGGCAGGACCTCTTTGA+TGG 0.408930 3_1:-5834602 None:intergenic
CAGTTATTGGTTGTGGCTGA+TGG 0.413622 3_1:+5834889 Msa0345990:three_prime_UTR
AGACATTGGAAAAGAAGAAA+AGG 0.418775 3_1:+5834674 Msa0345990:CDS
TTGAGACTAAATATAAAATC+AGG 0.420368 3_1:+5834710 Msa0345990:CDS
GGAGTACTTGTGGGCCCTGC+GGG 0.425415 3_1:+5834793 Msa0345990:CDS
TCTTTATTCCGCTGCACAGC+AGG 0.438536 3_1:-5834619 None:intergenic
AGACGATGCTATCGAATGAT+TGG 0.440454 3_1:+5834559 Msa0345990:CDS
TTCGATAGCATCGTCTAGAT+TGG 0.458077 3_1:-5834552 None:intergenic
ATATTTATTGCTTAAACAGT+GGG 0.490298 3_1:+5835220 Msa0345990:three_prime_UTR
ACAACCAATAACTGTAAAAG+AGG 0.496037 3_1:-5834880 None:intergenic
GACATTGGAAAAGAAGAAAA+GGG 0.500685 3_1:+5834675 Msa0345990:CDS
GTGTAGAATCATGGCTAGTA+AGG 0.508954 3_1:+5834375 Msa0345990:intron
GCTGAGGTGAACGATGAATC+TGG 0.515292 3_1:+5834748 Msa0345990:CDS
AATTTCATGTCATTCGTTTG+AGG 0.519470 3_1:-5834851 None:intergenic
CCAAATTTACACCAAAATAA+CGG 0.527386 3_1:+5834471 Msa0345990:CDS
TATATTTATTGCTTAAACAG+TGG 0.528982 3_1:+5835219 Msa0345990:three_prime_UTR
AGATTGGTCTAGCGGCTGAA+TGG 0.531922 3_1:-5834536 None:intergenic
TGTCATTCGCTACCACCCGC+AGG 0.539315 3_1:-5834808 None:intergenic
TATTCCGCTGCACAGCAGGC+AGG 0.540606 3_1:-5834615 None:intergenic
TATTGGTTGTGGCTGATGGA+TGG 0.541385 3_1:+5834893 Msa0345990:three_prime_UTR
TGCAAGGCTTAACGAGACAT+TGG 0.541862 3_1:+5834660 Msa0345990:CDS
GTACTTGTGGGCCCTGCGGG+TGG 0.543152 3_1:+5834796 Msa0345990:CDS
GAACGATGAATCTGGTAAGA+AGG 0.544140 3_1:+5834756 Msa0345990:CDS
TCAGCTCAAGGAGTACTTGT+GGG 0.554339 3_1:+5834784 Msa0345990:CDS
AAGAAGGAAGGTTCAGCTCA+AGG 0.558366 3_1:+5834772 Msa0345990:CDS
GCTGTGCAGCGGAATAAAGA+AGG 0.563818 3_1:+5834622 Msa0345990:CDS
CGATGCTATCGAATGATTGG+AGG 0.563926 3_1:+5834562 Msa0345990:CDS
AAAATTTAGTGTGTTGTGCA+GGG 0.569476 3_1:+5835171 Msa0345990:three_prime_UTR
TTCATCGTTCACCTCAGCAT+CGG 0.575473 3_1:-5834743 None:intergenic
GATGAATCTGGTAAGAAGGA+AGG 0.587326 3_1:+5834760 Msa0345990:CDS
CTAAATATAAAATCAGGATG+AGG 0.587775 3_1:+5834716 Msa0345990:CDS
GATGAGGAAGGCCGATGCTG+AGG 0.588771 3_1:+5834732 Msa0345990:CDS
AGGTCCTGCCTGCTGTGCAG+CGG 0.589956 3_1:+5834611 Msa0345990:CDS
GACTTGAAGAGGTTGTTGCA+AGG 0.593695 3_1:+5834644 Msa0345990:CDS
GTCATTCGCTACCACCCGCA+GGG 0.594608 3_1:-5834807 None:intergenic
ATATAAAATCAGGATGAGGA+AGG 0.596264 3_1:+5834720 Msa0345990:CDS
TAGTAAGGCCGAAGATCGCA+AGG 0.597879 3_1:+5834390 Msa0345990:CDS
AGGAGTACTTGTGGGCCCTG+CGG 0.600783 3_1:+5834792 Msa0345990:CDS
TTCAGCTCAAGGAGTACTTG+TGG 0.603923 3_1:+5834783 Msa0345990:CDS
AATGATTGGAGGGGTGCTTG+TGG 0.605277 3_1:+5834573 Msa0345990:CDS
GATGCTATCGAATGATTGGA+GGG 0.617244 3_1:+5834563 Msa0345990:CDS
GGTTGAAGATGCAAATATGC+TGG 0.619988 3_1:+5834995 Msa0345990:three_prime_UTR
GGAAGTCAGTGAGCACTCGT+TGG 0.621570 3_1:+5834504 Msa0345990:CDS
CAAATATGTATGCAGCTATG+AGG 0.631644 3_1:+5834437 Msa0345990:CDS
CATCGTCTAGATTGGTCTAG+CGG 0.643268 3_1:-5834544 None:intergenic
GAATAAAGAAGGACTTGAAG+AGG 0.645797 3_1:+5834633 Msa0345990:CDS
CAACCAATAACTGTAAAAGA+GGG 0.659909 3_1:-5834879 None:intergenic
AAATTTAGTGTGTTGTGCAG+GGG 0.664913 3_1:+5835172 Msa0345990:three_prime_UTR
TGTGGAAAGAACCATCAAAG+AGG 0.692372 3_1:+5834591 Msa0345990:CDS
ATGCTATCGAATGATTGGAG+GGG 0.721195 3_1:+5834564 Msa0345990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTTTTATATAAATCTT+TGG + chr3_1:5833543-5833562 Msa0345990:intron 10.0%
!!! TAGATTTTACAAATTTAATA+TGG - chr3_1:5833636-5833655 None:intergenic 10.0%
!!! TAATTGATTTTAGTTAAAAG+TGG + chr3_1:5831528-5831547 Msa0345990:intron 15.0%
!!! AATTGATTTTAGTTAAAAGT+GGG + chr3_1:5831529-5831548 Msa0345990:intron 15.0%
!!! GATAAAATTGTTTTATGTTT+TGG + chr3_1:5831557-5831576 Msa0345990:intron 15.0%
!!! TTTTTGTTATCTTAAACAAA+TGG + chr3_1:5832442-5832461 Msa0345990:intron 15.0%
!!! TTTGAAATAATAGCATTAAA+TGG - chr3_1:5832819-5832838 None:intergenic 15.0%
!! ACATTAAGGAATTTATTTAA+GGG - chr3_1:5832846-5832865 None:intergenic 15.0%
!! AACATTAAGGAATTTATTTA+AGG - chr3_1:5832847-5832866 None:intergenic 15.0%
!! TTATTGTGTATCTAATATTT+CGG - chr3_1:5833132-5833151 None:intergenic 15.0%
!! ACTAAATTCAAAAAATATTG+AGG - chr3_1:5833220-5833239 None:intergenic 15.0%
!! ATTGATAAACTAAAAAAAAG+GGG - chr3_1:5833435-5833454 None:intergenic 15.0%
!! CATTGATAAACTAAAAAAAA+GGG - chr3_1:5833436-5833455 None:intergenic 15.0%
!! ACATTGATAAACTAAAAAAA+AGG - chr3_1:5833437-5833456 None:intergenic 15.0%
!!! AAAAATGCAATTTTTTGTTC+TGG + chr3_1:5830702-5830721 Msa0345990:intron 20.0%
!! CTAGTAACAAACAAAAATAA+GGG - chr3_1:5830902-5830921 None:intergenic 20.0%
!!! CTTATTTTTGTTTGTTACTA+GGG + chr3_1:5830901-5830920 Msa0345990:intron 20.0%
!!! TTATTTTTGTTTGTTACTAG+GGG + chr3_1:5830902-5830921 Msa0345990:intron 20.0%
!!! ATAAGATTTTTTATCCAAAC+AGG - chr3_1:5830928-5830947 None:intergenic 20.0%
!!! GTTGTTTTTATAACCTAAAT+TGG + chr3_1:5831062-5831081 Msa0345990:intron 20.0%
!!! AACATTCTCTGTTTATTTTT+AGG + chr3_1:5831325-5831344 Msa0345990:intron 20.0%
!!! TTTTTAATAACACAAGCAAT+CGG - chr3_1:5832267-5832286 None:intergenic 20.0%
!! ACAAAAAAAAAAGCTCAAAA+TGG + chr3_1:5832398-5832417 Msa0345990:intron 20.0%
!! GCATTAGAATTCTATAATAA+GGG - chr3_1:5832918-5832937 None:intergenic 20.0%
!! TGCATTAGAATTCTATAATA+AGG - chr3_1:5832919-5832938 None:intergenic 20.0%
!! TATTGTGTATCTAATATTTC+GGG - chr3_1:5833131-5833150 None:intergenic 20.0%
!!! TTATATAAATCTTTGGTGTT+TGG + chr3_1:5833550-5833569 Msa0345990:intron 20.0%
!! TTAAAATTGTATTTGCTTCA+TGG + chr3_1:5833920-5833939 Msa0345990:intron 20.0%
!!! TATATTTTGAAGAACTCTTT+CGG + chr3_1:5834078-5834097 Msa0345990:intron 20.0%
!!! TTCTTGTATAGAGTTTTATA+TGG + chr3_1:5834108-5834127 Msa0345990:intron 20.0%
!! ATGACATATACATTCATAAT+CGG - chr3_1:5834190-5834209 None:intergenic 20.0%
!!! AGGATAATACTTAAGATTTT+CGG - chr3_1:5834294-5834313 None:intergenic 20.0%
!! TTGAGACTAAATATAAAATC+AGG + chr3_1:5834710-5834729 Msa0345990:CDS 20.0%
!! TTGAAACAAAATGCTAAATT+TGG + chr3_1:5835102-5835121 Msa0345990:three_prime_UTR 20.0%
!! AGTTTCATGTTAAAAATAGT+TGG + chr3_1:5835147-5835166 Msa0345990:three_prime_UTR 20.0%
!! TATATTTATTGCTTAAACAG+TGG + chr3_1:5835219-5835238 Msa0345990:three_prime_UTR 20.0%
!! ATATTTATTGCTTAAACAGT+GGG + chr3_1:5835220-5835239 Msa0345990:three_prime_UTR 20.0%
!!! AGGTTTTGATTTTTTGTTGT+TGG + chr3_1:5830541-5830560 Msa0345990:intron 25.0%
!!! GGTTTTGATTTTTTGTTGTT+GGG + chr3_1:5830542-5830561 Msa0345990:intron 25.0%
! AGTTGATTGAACTTAATGTT+AGG + chr3_1:5830652-5830671 Msa0345990:intron 25.0%
! AGATTGAACTATACTATAGA+AGG + chr3_1:5830817-5830836 Msa0345990:intron 25.0%
!! TTGTATGTTTGAAGTAGAAT+TGG + chr3_1:5830842-5830861 Msa0345990:intron 25.0%
! CCTAGTAACAAACAAAAATA+AGG - chr3_1:5830903-5830922 None:intergenic 25.0%
!!! CCTTATTTTTGTTTGTTACT+AGG + chr3_1:5830900-5830919 Msa0345990:intron 25.0%
! TTTCATAAGTTCTCCAATTT+AGG - chr3_1:5831078-5831097 None:intergenic 25.0%
!! AGTAAGTTGAAAAAAGCTTA+TGG + chr3_1:5831097-5831116 Msa0345990:intron 25.0%
!!! TAAGTTTTGTGTGACTATTT+GGG - chr3_1:5831153-5831172 None:intergenic 25.0%
!!! ATAAGTTTTGTGTGACTATT+TGG - chr3_1:5831154-5831173 None:intergenic 25.0%
!!! GAATTGTGTTACTGTTTTAT+TGG + chr3_1:5831637-5831656 Msa0345990:intron 25.0%
! TTTAAGTGATATCATAACAG+AGG + chr3_1:5832002-5832021 Msa0345990:intron 25.0%
! TTAAGTGATATCATAACAGA+GGG + chr3_1:5832003-5832022 Msa0345990:intron 25.0%
! ACATCTTATACAATAGTAGT+TGG - chr3_1:5832190-5832209 None:intergenic 25.0%
!! TTTTAATAACACAAGCAATC+GGG - chr3_1:5832266-5832285 None:intergenic 25.0%
!! TAGGTTTTCTTCTAACAAAA+TGG + chr3_1:5832317-5832336 Msa0345990:intron 25.0%
! TCTCATAAGCTAAATATAAC+AGG + chr3_1:5832372-5832391 Msa0345990:intron 25.0%
!! TCTAATGTATGAGTTTTACA+AGG + chr3_1:5832567-5832586 Msa0345990:intron 25.0%
! TCCTTTGATTACTAACATTT+GGG - chr3_1:5832633-5832652 None:intergenic 25.0%
! ATCCTTTGATTACTAACATT+TGG - chr3_1:5832634-5832653 None:intergenic 25.0%
! AACAAAATGCATGAATTTGT+TGG + chr3_1:5832762-5832781 Msa0345990:intron 25.0%
! GTCATCTCTTTATAACATTA+AGG - chr3_1:5832860-5832879 None:intergenic 25.0%
! TTAGAAGTTACAATTCTACT+GGG - chr3_1:5832960-5832979 None:intergenic 25.0%
! TTACTTCAAAGATCAGAAAA+AGG - chr3_1:5833607-5833626 None:intergenic 25.0%
! TTTCGATTTCATAATTTCCT+TGG + chr3_1:5833801-5833820 Msa0345990:intron 25.0%
! CTCCTATATTGATGAAAAAA+GGG - chr3_1:5833829-5833848 None:intergenic 25.0%
! ATCAAGGACAAAAAATTGAA+AGG - chr3_1:5833991-5834010 None:intergenic 25.0%
!!! TTTCAATTTTTTGTCCTTGA+TGG + chr3_1:5833990-5834009 Msa0345990:intron 25.0%
! AATCGGACAAATAACATTAA+AGG - chr3_1:5834173-5834192 None:intergenic 25.0%
!! AGAATAAACAAAAGTATGCT+AGG - chr3_1:5834314-5834333 None:intergenic 25.0%
! TTCTACACAAATTTACACTA+AGG - chr3_1:5834363-5834382 None:intergenic 25.0%
! TGTAAATTTGTGTAGAATCA+TGG + chr3_1:5834366-5834385 Msa0345990:intron 25.0%
! CCAAATTTACACCAAAATAA+CGG + chr3_1:5834471-5834490 Msa0345990:CDS 25.0%
! CTAAATATAAAATCAGGATG+AGG + chr3_1:5834716-5834735 Msa0345990:CDS 25.0%
! TGAAGTAAACTTATCTTCAA+TGG + chr3_1:5834974-5834993 Msa0345990:three_prime_UTR 25.0%
!! AATATAAGCATTGCAACTTA+TGG - chr3_1:5835205-5835224 None:intergenic 25.0%
GAAAAGTGATCTTAGAATGT+TGG + chr3_1:5830618-5830637 Msa0345990:intron 30.0%
TAAGCGCTTATGTATAAACT+TGG + chr3_1:5830979-5830998 Msa0345990:intron 30.0%
GATCTATAGTCTGTCTATAT+TGG - chr3_1:5831199-5831218 None:intergenic 30.0%
AGACTATAGATCAAATTCAC+AGG + chr3_1:5831207-5831226 Msa0345990:intron 30.0%
GACTATAGATCAAATTCACA+GGG + chr3_1:5831208-5831227 Msa0345990:intron 30.0%
TATCAACTAAAACTCACCTT+AGG - chr3_1:5831271-5831290 None:intergenic 30.0%
!! TTTTAGGAGCTTCTTTAGAA+AGG + chr3_1:5831341-5831360 Msa0345990:intron 30.0%
TCTAATTGATCACAGTTTCT+TGG + chr3_1:5831423-5831442 Msa0345990:intron 30.0%
TAACCAAACAATCACATAAC+AGG - chr3_1:5831465-5831484 None:intergenic 30.0%
TCTATTCTTCCTAATACACT+GGG - chr3_1:5831690-5831709 None:intergenic 30.0%
TTCTATTCTTCCTAATACAC+TGG - chr3_1:5831691-5831710 None:intergenic 30.0%
! ATGAGTTTGAGACTATATAG+CGG - chr3_1:5831855-5831874 None:intergenic 30.0%
TTTAAAAATCCAGCTAGACA+TGG - chr3_1:5832056-5832075 None:intergenic 30.0%
! ATGTCTAGCTGGATTTTTAA+AGG + chr3_1:5832055-5832074 Msa0345990:intron 30.0%
! TAAACAAATGGACTCAGTTT+AGG + chr3_1:5832454-5832473 Msa0345990:intron 30.0%
TTAGTAATCAAAGGATGTGT+GGG + chr3_1:5832638-5832657 Msa0345990:intron 30.0%
! TTTGTTGCTTATATGTGAGT+AGG - chr3_1:5832748-5832767 None:intergenic 30.0%
AGAGATGACTATTCATAAGT+AGG + chr3_1:5832871-5832890 Msa0345990:intron 30.0%
GTTAGAAGTTACAATTCTAC+TGG - chr3_1:5832961-5832980 None:intergenic 30.0%
! AAGAACTATCATCCTGATTT+TGG - chr3_1:5833695-5833714 None:intergenic 30.0%
!!! CTCCCTTTTTTCATCAATAT+AGG + chr3_1:5833824-5833843 Msa0345990:intron 30.0%
GCTCCTATATTGATGAAAAA+AGG - chr3_1:5833830-5833849 None:intergenic 30.0%
!! GCTTTATTTGTTGTTTGTGT+TGG + chr3_1:5833942-5833961 Msa0345990:intron 30.0%
TCATAATCAGATTACCATCA+AGG - chr3_1:5834007-5834026 None:intergenic 30.0%
!! TTTTGGTGTAAATTTGGTTG+AGG - chr3_1:5834468-5834487 None:intergenic 30.0%
!!! CCGTTATTTTGGTGTAAATT+TGG - chr3_1:5834474-5834493 None:intergenic 30.0%
!!! CATTTCAAGTTCCGTTATTT+TGG - chr3_1:5834485-5834504 None:intergenic 30.0%
! CAAAATAACGGAACTTGAAA+TGG + chr3_1:5834483-5834502 Msa0345990:CDS 30.0%
AGACATTGGAAAAGAAGAAA+AGG + chr3_1:5834674-5834693 Msa0345990:CDS 30.0%
GACATTGGAAAAGAAGAAAA+GGG + chr3_1:5834675-5834694 Msa0345990:CDS 30.0%
ATATAAAATCAGGATGAGGA+AGG + chr3_1:5834720-5834739 Msa0345990:CDS 30.0%
AATTTCATGTCATTCGTTTG+AGG - chr3_1:5834854-5834873 None:intergenic 30.0%
CAACCAATAACTGTAAAAGA+GGG - chr3_1:5834882-5834901 None:intergenic 30.0%
ACAACCAATAACTGTAAAAG+AGG - chr3_1:5834883-5834902 None:intergenic 30.0%
!! TCTTTTACAGTTATTGGTTG+TGG + chr3_1:5834882-5834901 Msa0345990:three_prime_UTR 30.0%
AAAAATTTAGTGTGTTGTGC+AGG + chr3_1:5835170-5835189 Msa0345990:three_prime_UTR 30.0%
AAAATTTAGTGTGTTGTGCA+GGG + chr3_1:5835171-5835190 Msa0345990:three_prime_UTR 30.0%
!! GTGCTTGTTTGTTTCTGAAA+TGG + chr3_1:5830864-5830883 Msa0345990:intron 35.0%
CTATTACGAGAGTAACTATC+TGG + chr3_1:5831390-5831409 Msa0345990:intron 35.0%
AGTTTCTTGGTGAAAAGTTG+TGG + chr3_1:5831436-5831455 Msa0345990:intron 35.0%
TTGCCTGTTATGTGATTGTT+TGG + chr3_1:5831459-5831478 Msa0345990:intron 35.0%
GTGATTGTTTGGTTATACAG+TGG + chr3_1:5831470-5831489 Msa0345990:intron 35.0%
!! TATGTTTTGGATATGTTCCC+TGG + chr3_1:5831570-5831589 Msa0345990:intron 35.0%
ATAGAAACCAAATACAACGC+AGG + chr3_1:5831705-5831724 Msa0345990:intron 35.0%
TAGAAACCAAATACAACGCA+GGG + chr3_1:5831706-5831725 Msa0345990:intron 35.0%
AAGTGTGTCAAATATGTGTG+AGG - chr3_1:5831816-5831835 None:intergenic 35.0%
! TATATAGCGGAATGTGTGTA+CGG - chr3_1:5831842-5831861 None:intergenic 35.0%
!! TAGCTGGATTTTTAAAGGCT+AGG + chr3_1:5832060-5832079 Msa0345990:intron 35.0%
!! AGCTGGATTTTTAAAGGCTA+GGG + chr3_1:5832061-5832080 Msa0345990:intron 35.0%
TCCTTAATGAATTCGTCTAG+CGG + chr3_1:5832139-5832158 Msa0345990:intron 35.0%
!! ATAGTAGTTGGTAGCTTCTA+CGG - chr3_1:5832178-5832197 None:intergenic 35.0%
GCTGAATATGGGTAATAAAG+AGG - chr3_1:5832230-5832249 None:intergenic 35.0%
CATATTTCGTGGCTGAATAT+GGG - chr3_1:5832241-5832260 None:intergenic 35.0%
CATATTCAGCCACGAAATAT+GGG + chr3_1:5832240-5832259 Msa0345990:intron 35.0%
AACTATATTATGCTGACCAC+AGG - chr3_1:5832511-5832530 None:intergenic 35.0%
GCCCAAATGTTAGTAATCAA+AGG + chr3_1:5832629-5832648 Msa0345990:intron 35.0%
GTTAGTAATCAAAGGATGTG+TGG + chr3_1:5832637-5832656 Msa0345990:intron 35.0%
CAACAGGATTCAATAAGATG+CGG - chr3_1:5833022-5833041 None:intergenic 35.0%
TGTGTACTTTCAAACACAAC+AGG - chr3_1:5833038-5833057 None:intergenic 35.0%
GACATGACACAGCTATAATA+TGG - chr3_1:5833198-5833217 None:intergenic 35.0%
TTAGTTTATCAATGTGTCCG+AGG + chr3_1:5833235-5833254 Msa0345990:intron 35.0%
TAGTTTATCAATGTGTCCGA+GGG + chr3_1:5833236-5833255 Msa0345990:intron 35.0%
TAAAGTTGTTGTCATCTGAC+TGG + chr3_1:5833278-5833297 Msa0345990:intron 35.0%
TACAATACACCAAAGATGGT+GGG + chr3_1:5833361-5833380 Msa0345990:intron 35.0%
AGGTATACAACATGTGAGAT+GGG - chr3_1:5833477-5833496 None:intergenic 35.0%
ATGTTGTATACCTGCTTTCA+AGG + chr3_1:5833484-5833503 Msa0345990:intron 35.0%
GACGCTAACATTAACATTAC+AGG - chr3_1:5833667-5833686 None:intergenic 35.0%
! TATCATCCTGATTTTGGACA+TGG - chr3_1:5833689-5833708 None:intergenic 35.0%
CAAATATGTATGCAGCTATG+AGG + chr3_1:5834437-5834456 Msa0345990:CDS 35.0%
GAATAAAGAAGGACTTGAAG+AGG + chr3_1:5834633-5834652 Msa0345990:CDS 35.0%
AAATTTAGTGTGTTGTGCAG+GGG + chr3_1:5835172-5835191 Msa0345990:three_prime_UTR 35.0%
GAGAGTAACTATCTGGTTGT+TGG + chr3_1:5831397-5831416 Msa0345990:intron 40.0%
TGACCAATTCCCAGTGTATT+AGG + chr3_1:5831678-5831697 Msa0345990:intron 40.0%
CTTCCTAATACACTGGGAAT+TGG - chr3_1:5831684-5831703 None:intergenic 40.0%
TATAGTCCCTGCGTTGTATT+TGG - chr3_1:5831715-5831734 None:intergenic 40.0%
TGCTAGGTAGAAGAATAAGC+AGG + chr3_1:5831754-5831773 Msa0345990:intron 40.0%
GCTAGGTAGAAGAATAAGCA+GGG + chr3_1:5831755-5831774 Msa0345990:intron 40.0%
CTAGGTAGAAGAATAAGCAG+GGG + chr3_1:5831756-5831775 Msa0345990:intron 40.0%
!! TAGTACCAACTTGCTGACTT+GGG + chr3_1:5831975-5831994 Msa0345990:intron 40.0%
AAAAACCCAAGTCAGCAAGT+TGG - chr3_1:5831983-5832002 None:intergenic 40.0%
GTCATATATCCATGTCTAGC+TGG + chr3_1:5832044-5832063 Msa0345990:intron 40.0%
TGCTATGCAGCTAAAGAAGT+TGG - chr3_1:5832102-5832121 None:intergenic 40.0%
CCTTAATGAATTCGTCTAGC+GGG + chr3_1:5832140-5832159 Msa0345990:intron 40.0%
CCATATTTCGTGGCTGAATA+TGG - chr3_1:5832242-5832261 None:intergenic 40.0%
CCATATTCAGCCACGAAATA+TGG + chr3_1:5832239-5832258 Msa0345990:intron 40.0%
ATATTATGCTGACCACAGGA+TGG - chr3_1:5832507-5832526 None:intergenic 40.0%
ATTGCATTCGCTTAGCAGAA+AGG + chr3_1:5832604-5832623 Msa0345990:intron 40.0%
AGGGAATGATGTACTCACTT+CGG - chr3_1:5832899-5832918 None:intergenic 40.0%
AGTTTATCAATGTGTCCGAG+GGG + chr3_1:5833237-5833256 Msa0345990:intron 40.0%
AGCCTCTAGTGTCAAAAACA+GGG + chr3_1:5833328-5833347 Msa0345990:intron 40.0%
!!! TACCCTGTTTTTGACACTAG+AGG - chr3_1:5833333-5833352 None:intergenic 40.0%
CTAGTGTCAAAAACAGGGTA+AGG + chr3_1:5833333-5833352 Msa0345990:intron 40.0%
TGCGTACAATACACCAAAGA+TGG + chr3_1:5833357-5833376 Msa0345990:intron 40.0%
GTACAATACACCAAAGATGG+TGG + chr3_1:5833360-5833379 Msa0345990:intron 40.0%
ACTAAAAAAAAGGGGCAACC+CGG - chr3_1:5833427-5833446 None:intergenic 40.0%
CAGGTATACAACATGTGAGA+TGG - chr3_1:5833478-5833497 None:intergenic 40.0%
!! TTTGGTGTTTGGTGTCTGAT+TGG + chr3_1:5833561-5833580 Msa0345990:intron 40.0%
!! TTGGTGTTTGGTGTCTGATT+GGG + chr3_1:5833562-5833581 Msa0345990:intron 40.0%
TTGATGAAAAAAGGGAGCCA+AGG - chr3_1:5833821-5833840 None:intergenic 40.0%
TACTTGTGTCTCTGTATGCT+AGG + chr3_1:5833895-5833914 Msa0345990:intron 40.0%
! TGTTGTTTGTGTTGGTCTGT+TGG + chr3_1:5833950-5833969 Msa0345990:intron 40.0%
GTGTAGAATCATGGCTAGTA+AGG + chr3_1:5834375-5834394 Msa0345990:intron 40.0%
GCAACTGCTTGTTCATTTAC+TGG - chr3_1:5834418-5834437 None:intergenic 40.0%
TTCGATAGCATCGTCTAGAT+TGG - chr3_1:5834555-5834574 None:intergenic 40.0%
AGACGATGCTATCGAATGAT+TGG + chr3_1:5834559-5834578 Msa0345990:CDS 40.0%
GATGCTATCGAATGATTGGA+GGG + chr3_1:5834563-5834582 Msa0345990:CDS 40.0%
ATGCTATCGAATGATTGGAG+GGG + chr3_1:5834564-5834583 Msa0345990:CDS 40.0%
TGTGGAAAGAACCATCAAAG+AGG + chr3_1:5834591-5834610 Msa0345990:CDS 40.0%
GAACGATGAATCTGGTAAGA+AGG + chr3_1:5834756-5834775 Msa0345990:CDS 40.0%
GATGAATCTGGTAAGAAGGA+AGG + chr3_1:5834760-5834779 Msa0345990:CDS 40.0%
! CTGCCCTCTTTTACAGTTAT+TGG + chr3_1:5834876-5834895 Msa0345990:three_prime_UTR 40.0%
!! CTGATGGATGGTTTTTGTAG+AGG + chr3_1:5834905-5834924 Msa0345990:three_prime_UTR 40.0%
GGTTGAAGATGCAAATATGC+TGG + chr3_1:5834995-5835014 Msa0345990:three_prime_UTR 40.0%
TTTGTTACTAGGGGCCTGTT+TGG + chr3_1:5830911-5830930 Msa0345990:intron 45.0%
TTACACTTTCCAGCTACAGC+AGG - chr3_1:5831598-5831617 None:intergenic 45.0%
GCAGGGACTATAGAAGAAGT+GGG + chr3_1:5831723-5831742 Msa0345990:intron 45.0%
GAAGTGGGAGATAAGATGCT+AGG + chr3_1:5831738-5831757 Msa0345990:intron 45.0%
AAAGAAGAATCCCGATCCAG+AGG + chr3_1:5831779-5831798 Msa0345990:intron 45.0%
AAGAAGAATCCCGATCCAGA+GGG + chr3_1:5831780-5831799 Msa0345990:intron 45.0%
!! GTAGTACCAACTTGCTGACT+TGG + chr3_1:5831974-5831993 Msa0345990:intron 45.0%
CCCGCTAGACGAATTCATTA+AGG - chr3_1:5832143-5832162 None:intergenic 45.0%
!! TTCTGACTTGTTCCATCCTG+TGG + chr3_1:5832492-5832511 Msa0345990:intron 45.0%
TCAAAGGATGTGTGGGATAG+AGG + chr3_1:5832645-5832664 Msa0345990:intron 45.0%
ATGTGTGGGATAGAGGTCAT+AGG + chr3_1:5832652-5832671 Msa0345990:intron 45.0%
TGTGTGGGATAGAGGTCATA+GGG + chr3_1:5832653-5832672 Msa0345990:intron 45.0%
GGATAGAGGTCATAGGGTAA+GGG + chr3_1:5832659-5832678 Msa0345990:intron 45.0%
GTTGTTGTCATCTGACTGGA+AGG + chr3_1:5833282-5833301 Msa0345990:intron 45.0%
CAGCCTCTAGTGTCAAAAAC+AGG + chr3_1:5833327-5833346 Msa0345990:intron 45.0%
ACATGTGAGATGGGTGCAAA+AGG - chr3_1:5833468-5833487 None:intergenic 45.0%
! ATACCTGCTTTCAAGGTGTC+TGG + chr3_1:5833491-5833510 Msa0345990:intron 45.0%
TAGCGTCCATGTCCAAAATC+AGG + chr3_1:5833680-5833699 Msa0345990:intron 45.0%
TGAAGAACTCTTTCGGTCTG+AGG + chr3_1:5834085-5834104 Msa0345990:intron 45.0%
!! CATCGTCTAGATTGGTCTAG+CGG - chr3_1:5834547-5834566 None:intergenic 45.0%
CGATGCTATCGAATGATTGG+AGG + chr3_1:5834562-5834581 Msa0345990:CDS 45.0%
GACTTGAAGAGGTTGTTGCA+AGG + chr3_1:5834644-5834663 Msa0345990:CDS 45.0%
TGCAAGGCTTAACGAGACAT+TGG + chr3_1:5834660-5834679 Msa0345990:CDS 45.0%
TTCATCGTTCACCTCAGCAT+CGG - chr3_1:5834746-5834765 None:intergenic 45.0%
AAGAAGGAAGGTTCAGCTCA+AGG + chr3_1:5834772-5834791 Msa0345990:CDS 45.0%
!! TTCAGCTCAAGGAGTACTTG+TGG + chr3_1:5834783-5834802 Msa0345990:CDS 45.0%
!! TCAGCTCAAGGAGTACTTGT+GGG + chr3_1:5834784-5834803 Msa0345990:CDS 45.0%
! CAGTTATTGGTTGTGGCTGA+TGG + chr3_1:5834889-5834908 Msa0345990:three_prime_UTR 45.0%
! TATTGGTTGTGGCTGATGGA+TGG + chr3_1:5834893-5834912 Msa0345990:three_prime_UTR 45.0%
!! TATTATTATAATAAAAATGT+AGG + chr3_1:5832298-5832317 Msa0345990:intron 5.0%
CGAGTCTCGCTCTATTTGGT+GGG - chr3_1:5831235-5831254 None:intergenic 50.0%
TCGAGTCTCGCTCTATTTGG+TGG - chr3_1:5831236-5831255 None:intergenic 50.0%
CCATCGAGTCTCGCTCTATT+TGG - chr3_1:5831239-5831258 None:intergenic 50.0%
CCAAATAGAGCGAGACTCGA+TGG + chr3_1:5831236-5831255 Msa0345990:intron 50.0%
TCGATGGTTCGTTGGACCTA+AGG + chr3_1:5831252-5831271 Msa0345990:intron 50.0%
CGCAGGGACTATAGAAGAAG+TGG + chr3_1:5831722-5831741 Msa0345990:intron 50.0%
TAAACCTCCTCCCTCTGGAT+CGG - chr3_1:5831793-5831812 None:intergenic 50.0%
CAATACACACAAGCGCAGTG+AGG - chr3_1:5831896-5831915 None:intergenic 50.0%
GGGATAGAGGTCATAGGGTA+AGG + chr3_1:5832658-5832677 Msa0345990:intron 50.0%
CAACTTTACCAGTTCCGCCA+AGG - chr3_1:5833266-5833285 None:intergenic 50.0%
GTCATCTGACTGGAAGGTCA+CGG + chr3_1:5833288-5833307 Msa0345990:intron 50.0%
TCATCTGACTGGAAGGTCAC+GGG + chr3_1:5833289-5833308 Msa0345990:intron 50.0%
! TGACACTAGAGGCTGTTTCC+AGG - chr3_1:5833322-5833341 None:intergenic 50.0%
GAGATGGGTGCAAAAGGACA+CGG - chr3_1:5833462-5833481 None:intergenic 50.0%
ATAGCGAAGTGCAGTGCAAG+TGG - chr3_1:5833517-5833536 None:intergenic 50.0%
TAGTAAGGCCGAAGATCGCA+AGG + chr3_1:5834390-5834409 Msa0345990:CDS 50.0%
!! TACTGGCTCCTTGCGATCTT+CGG - chr3_1:5834401-5834420 None:intergenic 50.0%
!! AGATTGGTCTAGCGGCTGAA+TGG - chr3_1:5834539-5834558 None:intergenic 50.0%
!! AATGATTGGAGGGGTGCTTG+TGG + chr3_1:5834573-5834592 Msa0345990:CDS 50.0%
TCTTTATTCCGCTGCACAGC+AGG - chr3_1:5834622-5834641 None:intergenic 50.0%
GCTGTGCAGCGGAATAAAGA+AGG + chr3_1:5834622-5834641 Msa0345990:CDS 50.0%
GCTGAGGTGAACGATGAATC+TGG + chr3_1:5834748-5834767 Msa0345990:CDS 50.0%
AGCGAGACTCGATGGTTCGT+TGG + chr3_1:5831244-5831263 Msa0345990:intron 55.0%
AAGAATCCCGATCCAGAGGG+AGG + chr3_1:5831783-5831802 Msa0345990:intron 55.0%
AAACCTCCTCCCTCTGGATC+GGG - chr3_1:5831792-5831811 None:intergenic 55.0%
TGAGGTAAACCTCCTCCCTC+TGG - chr3_1:5831798-5831817 None:intergenic 55.0%
GCAATCGGGCCCATATTTCG+TGG - chr3_1:5832252-5832271 None:intergenic 55.0%
ATGTGTCCGAGGGGTAACCT+TGG + chr3_1:5833246-5833265 Msa0345990:intron 55.0%
AAGGTCACGGGTTCAAGTCC+TGG + chr3_1:5833301-5833320 Msa0345990:intron 55.0%
CTAAAGCTCCCGCATACGCA+GGG - chr3_1:5833400-5833419 None:intergenic 55.0%
ACTAAAGCTCCCGCATACGC+AGG - chr3_1:5833401-5833420 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr3_1:5833405-5833424 Msa0345990:intron 55.0%
TGGCCAGACACCTTGAAAGC+AGG - chr3_1:5833497-5833516 None:intergenic 55.0%
GGAAGTCAGTGAGCACTCGT+TGG + chr3_1:5834504-5834523 Msa0345990:CDS 55.0%
AGCAGGCAGGACCTCTTTGA+TGG - chr3_1:5834605-5834624 None:intergenic 55.0%
TCCAGCTACAGCAGGTGCCA+GGG - chr3_1:5831590-5831609 None:intergenic 60.0%
TTCCAGCTACAGCAGGTGCC+AGG - chr3_1:5831591-5831610 None:intergenic 60.0%
AATCCCGATCCAGAGGGAGG+AGG + chr3_1:5831786-5831805 Msa0345990:intron 60.0%
CACACAAGCGCAGTGAGGAG+AGG - chr3_1:5831891-5831910 None:intergenic 60.0%
TGTCCGAGGGGTAACCTTGG+CGG + chr3_1:5833249-5833268 Msa0345990:intron 60.0%
GTTCCGCCAAGGTTACCCCT+CGG - chr3_1:5833255-5833274 None:intergenic 60.0%
AGGGGTAACCTTGGCGGAAC+TGG + chr3_1:5833255-5833274 Msa0345990:intron 60.0%
GGAAGGGGTCCCACCATCTT+TGG - chr3_1:5833373-5833392 None:intergenic 60.0%
CATACGCAGGGTTCGGGAAG+GGG - chr3_1:5833388-5833407 None:intergenic 60.0%
GCATACGCAGGGTTCGGGAA+GGG - chr3_1:5833389-5833408 None:intergenic 60.0%
CTTCCCGAACCCTGCGTATG+CGG + chr3_1:5833388-5833407 Msa0345990:intron 60.0%
TTCCCGAACCCTGCGTATGC+GGG + chr3_1:5833389-5833408 Msa0345990:intron 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr3_1:5833406-5833425 Msa0345990:intron 60.0%
TATTCCGCTGCACAGCAGGC+AGG - chr3_1:5834618-5834637 None:intergenic 60.0%
GATGAGGAAGGCCGATGCTG+AGG + chr3_1:5834732-5834751 Msa0345990:CDS 60.0%
!! AGGAGTACTTGTGGGCCCTG+CGG + chr3_1:5834792-5834811 Msa0345990:CDS 60.0%
GTCATTCGCTACCACCCGCA+GGG - chr3_1:5834810-5834829 None:intergenic 60.0%
TGTCATTCGCTACCACCCGC+AGG - chr3_1:5834811-5834830 None:intergenic 60.0%
TCCCTGGCACCTGCTGTAGC+TGG + chr3_1:5831586-5831605 Msa0345990:intron 65.0%
CGCATACGCAGGGTTCGGGA+AGG - chr3_1:5833390-5833409 None:intergenic 65.0%
CTCCCGCATACGCAGGGTTC+GGG - chr3_1:5833394-5833413 None:intergenic 65.0%
GCTCCCGCATACGCAGGGTT+CGG - chr3_1:5833395-5833414 None:intergenic 65.0%
AGGTCCTGCCTGCTGTGCAG+CGG + chr3_1:5834611-5834630 Msa0345990:CDS 65.0%
! GGAGTACTTGTGGGCCCTGC+GGG + chr3_1:5834793-5834812 Msa0345990:CDS 65.0%
GTACTTGTGGGCCCTGCGGG+TGG + chr3_1:5834796-5834815 Msa0345990:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 5830536 5835286 5830536 ID=Msa0345990;Name=Msa0345990
chr3_1 mRNA 5830536 5835286 5830536 ID=Msa0345990-mRNA-1;Parent=Msa0345990;Name=Msa0345990-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0.33|0.33|1|1|3|458|156
chr3_1 exon 5830536 5830542 5830536 ID=Msa0345990-mRNA-1:exon:1703;Parent=Msa0345990-mRNA-1
chr3_1 exon 5831347 5831362 5831347 ID=Msa0345990-mRNA-1:exon:1704;Parent=Msa0345990-mRNA-1
chr3_1 exon 5834381 5835286 5834381 ID=Msa0345990-mRNA-1:exon:1705;Parent=Msa0345990-mRNA-1
chr3_1 CDS 5830536 5830542 5830536 ID=Msa0345990-mRNA-1:cds;Parent=Msa0345990-mRNA-1
chr3_1 CDS 5831347 5831362 5831347 ID=Msa0345990-mRNA-1:cds;Parent=Msa0345990-mRNA-1
chr3_1 CDS 5834381 5834828 5834381 ID=Msa0345990-mRNA-1:cds;Parent=Msa0345990-mRNA-1
chr3_1 three_prime_UTR 5834829 5835286 5834829 ID=Msa0345990-mRNA-1:three_prime_utr;Parent=Msa0345990-mRNA-1
Gene Sequence

>Msa0345990

ATGTTAGGAGCTTCTTTAGAAAGAATCATGGCTAGTAAGGCCGAAGATCGCAAGGAGCCAGTAAATGAACAAGCAGTTGCAAATATGTATGCAGCTATGAGGTCAGACCTCAACCAAATTTACACCAAAATAACGGAACTTGAAATGGAAGTCAGTGAGCACTCGTTGGTTCTTAATGCCATTCAGCCGCTAGACCAATCTAGACGATGCTATCGAATGATTGGAGGGGTGCTTGTGGAAAGAACCATCAAAGAGGTCCTGCCTGCTGTGCAGCGGAATAAAGAAGGACTTGAAGAGGTTGTTGCAAGGCTTAACGAGACATTGGAAAAGAAGAAAAGGGAAATTGCTGAGTTTGAGACTAAATATAAAATCAGGATGAGGAAGGCCGATGCTGAGGTGAACGATGAATCTGGTAAGAAGGAAGGTTCAGCTCAAGGAGTACTTGTGGGCCCTGCGGGTGGTAGCGAATGA
Protein sequence

>Msa0345990

MLGASLERIMASKAEDRKEPVNEQAVANMYAAMRSDLNQIYTKITELEMEVSEHSLVLNAIQPLDQSRRCYRMIGGVLVERTIKEVLPAVQRNKEGLEEVVARLNETLEKKKREIAEFETKYKIRMRKADAEVNDESGKKEGSAQGVLVGPAGGSE*