AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0358540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0358540 MtrunA17_Chr4g0057861 96.667 30 1 0 65 94 88 117 8.09e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTATATTGAATGTATT+TGG 0.141751 3_1:-43565774 Msa0358540:three_prime_UTR
AACTATCCCTATACAAGATT+TGG 0.236483 3_1:+43565684 None:intergenic
CGATGTCTGTTTGTTGGTTC+TGG 0.264292 3_1:-43568142 Msa0358540:CDS
TCTTCCCCAAATCTTGTATA+GGG 0.283293 3_1:-43565690 Msa0358540:three_prime_UTR
TTCTTCCCCAAATCTTGTAT+AGG 0.311667 3_1:-43565691 Msa0358540:three_prime_UTR
GCAACGACGACCGGGGAAAA+GGG 0.326475 3_1:+43568236 None:intergenic
ACTATCCCTATACAAGATTT+GGG 0.340396 3_1:+43565685 None:intergenic
TTGCCATGCACTCTTCTCAT+TGG 0.355246 3_1:-43565733 Msa0358540:three_prime_UTR
TATATGATATTTCATATTAG+TGG 0.360869 3_1:-43565524 Msa0358540:three_prime_UTR
GGCGCCGTCACTTGTGTTCC+AGG 0.366663 3_1:-43568094 Msa0358540:intron
TTATTGGTATCTAGATTAAT+CGG 0.368659 3_1:-43565981 Msa0358540:intron
GGAGCGGCGGCGCTTGGTGG+TGG 0.374555 3_1:-43568115 Msa0358540:CDS
GGTAAGGGAGCGGCGGCGCT+TGG 0.374889 3_1:-43568121 Msa0358540:CDS
TCTCCGCCAGCTTCGAGGTT+TGG 0.380857 3_1:-43568190 Msa0358540:CDS
AGCAACGACGACCGGGGAAA+AGG 0.394247 3_1:+43568235 None:intergenic
CAAAGCTGCTGCTCATTTGA+AGG 0.396278 3_1:-43567373 Msa0358540:intron
CTGGCGGAGAGATGTTTATC+TGG 0.409007 3_1:+43568203 None:intergenic
CACTATGGACTTCACTTGTA+AGG 0.416081 3_1:+43566282 None:intergenic
CCATGCACTCTTCTCATTGG+TGG 0.425389 3_1:-43565730 Msa0358540:three_prime_UTR
TCTGTTTGTTGGTTCTGGTA+AGG 0.437103 3_1:-43568137 Msa0358540:CDS
TTTGATATTGTAACAATGAC+AGG 0.437242 3_1:-43565565 Msa0358540:three_prime_UTR
CTGTTTGTTGGTTCTGGTAA+GGG 0.445106 3_1:-43568136 Msa0358540:CDS
CCTCTTGCTTCATAGCACTA+TGG 0.447376 3_1:+43566267 None:intergenic
TGAATGTATTTGGCTAAGAA+TGG 0.447776 3_1:-43565764 Msa0358540:three_prime_UTR
AGCAGCAGCTTTGTCCTTGT+CGG 0.451014 3_1:+43567383 None:intergenic
AGTATGTCAAGGCTTCAAAT+TGG 0.456191 3_1:-43565812 Msa0358540:three_prime_UTR
GGGTTCCGATGTCTGTTTGT+TGG 0.464754 3_1:-43568148 Msa0358540:CDS
AAGGGAGCGGCGGCGCTTGG+TGG 0.467738 3_1:-43568118 Msa0358540:CDS
ATCTTGTATAGGGATAGTTT+AGG 0.468140 3_1:-43565680 Msa0358540:three_prime_UTR
ACATCTCTCCGCCAGCTTCG+AGG 0.471860 3_1:-43568195 Msa0358540:CDS
AAGAAAGAAGCAACGACGAC+CGG 0.471942 3_1:+43568227 None:intergenic
CAACGACGACCGGGGAAAAG+GGG 0.474597 3_1:+43568237 None:intergenic
AAACTACCAAACCTCGAAGC+TGG 0.490758 3_1:+43568184 None:intergenic
AGAAAGAAGCAACGACGACC+GGG 0.497113 3_1:+43568228 None:intergenic
ATACAAGATTTGGGGAAGAA+AGG 0.497353 3_1:+43565694 None:intergenic
GAAAGAAGCAACGACGACCG+GGG 0.539471 3_1:+43568229 None:intergenic
AAGCATAGTTACTAACATAG+AGG 0.540904 3_1:+43565640 None:intergenic
TAGATTAATCGGAAACATGC+AGG 0.544910 3_1:-43565970 Msa0358540:CDS
GAGATCCCATGGGCTAGCAC+TGG 0.550739 3_1:-43567441 Msa0358540:CDS
TGTTGGTTCTGGTAAGGGAG+CGG 0.561377 3_1:-43568131 Msa0358540:CDS
AACGACGACCGGGGAAAAGG+GGG 0.561868 3_1:+43568238 None:intergenic
GGAACCCATAAGAGATCCCA+TGG 0.565358 3_1:-43567452 Msa0358540:intron
AAGAGTGCATGGCAAGACCT+TGG 0.567658 3_1:+43565741 None:intergenic
TCAGCACCAGTGCTAGCCCA+TGG 0.575207 3_1:+43567435 None:intergenic
CAGCACCAGTGCTAGCCCAT+GGG 0.602177 3_1:+43567436 None:intergenic
AAGATTTGGGGAAGAAAGGA+AGG 0.607762 3_1:+43565698 None:intergenic
CCATAGTGCTATGAAGCAAG+AGG 0.610584 3_1:-43566267 Msa0358540:intron
ACAACCTGGAACACAAGTGA+CGG 0.611365 3_1:+43568090 None:intergenic
CCACCAATGAGAAGAGTGCA+TGG 0.616211 3_1:+43565730 None:intergenic
TATTTGGCTAAGAATGGCCA+AGG 0.618221 3_1:-43565758 Msa0358540:three_prime_UTR
AGAGCTTGTTGATGAAACAC+AGG 0.624120 3_1:-43565947 Msa0358540:CDS
TGGTTCTGGTAAGGGAGCGG+CGG 0.632893 3_1:-43568128 Msa0358540:CDS
CAGAACCAACAAACAGACAT+CGG 0.632902 3_1:+43568143 None:intergenic
CTACCAAACCTCGAAGCTGG+CGG 0.659472 3_1:+43568187 None:intergenic
CTATCCCTATACAAGATTTG+GGG 0.661248 3_1:+43565686 None:intergenic
GAACCCATAAGAGATCCCAT+GGG 0.734484 3_1:-43567451 Msa0358540:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATATCTTAAAATGTTT+CGG - chr3_1:43567065-43567084 Msa0358540:intron 10.0%
!! AATTAAATGAATGAATATTA+TGG + chr3_1:43567752-43567771 None:intergenic 10.0%
!! GTTAGTATAATATTGATATT+TGG - chr3_1:43566267-43566286 Msa0358540:intron 15.0%
!!! AAATTAAAATTGTTTTGTGT+GGG + chr3_1:43567408-43567427 None:intergenic 15.0%
!! ATATAATACTTATTGACATA+GGG - chr3_1:43567595-43567614 Msa0358540:intron 15.0%
!! GAGATAAAAAATTAATATCA+AGG + chr3_1:43567783-43567802 None:intergenic 15.0%
!!! AATTTTTTATCTCCATTTAT+TGG - chr3_1:43567790-43567809 Msa0358540:intron 15.0%
!! ATCTTTATATTGAATGTATT+TGG - chr3_1:43568013-43568032 Msa0358540:intron 15.0%
!! TATATGATATTTCATATTAG+TGG - chr3_1:43568263-43568282 Msa0358540:CDS 15.0%
!!! GTTTTTCTTGTTTTTATTGT+CGG - chr3_1:43565937-43565956 Msa0358540:CDS 20.0%
!! AAAACAATATTATACCCTTT+CGG + chr3_1:43566162-43566181 None:intergenic 20.0%
!! TAATGTTTATATATGCATCT+AGG - chr3_1:43566213-43566232 Msa0358540:intron 20.0%
!!! CTATTTTCTCAAATTATTAC+CGG - chr3_1:43567119-43567138 Msa0358540:intron 20.0%
!!! TTCAAAAGTTTGTTATGAAA+TGG - chr3_1:43567260-43567279 Msa0358540:intron 20.0%
!!! AATTAAAATTGTTTTGTGTG+GGG + chr3_1:43567407-43567426 None:intergenic 20.0%
!!! CAAATTAAAATTGTTTTGTG+TGG + chr3_1:43567409-43567428 None:intergenic 20.0%
!!! TATTTGTTTGAATTGGTTTT+AGG - chr3_1:43567436-43567455 Msa0358540:CDS 20.0%
!! CATATAATACTTATTGACAT+AGG - chr3_1:43567594-43567613 Msa0358540:intron 20.0%
!! TATAATACTTATTGACATAG+GGG - chr3_1:43567596-43567615 Msa0358540:intron 20.0%
!! TTAATCTAGATACCAATAAA+TGG + chr3_1:43567805-43567824 None:intergenic 20.0%
!! TTATTGGTATCTAGATTAAT+CGG - chr3_1:43567806-43567825 Msa0358540:intron 20.0%
!!! GGTTTTATATATTGTAACTT+TGG - chr3_1:43568194-43568213 Msa0358540:CDS 20.0%
! AACATAAACAAAAAACGTAC+CGG + chr3_1:43566023-43566042 None:intergenic 25.0%
! AAACAATATTATACCCTTTC+GGG + chr3_1:43566161-43566180 None:intergenic 25.0%
!!! TGTCTATTTTTTTGTGCTTT+AGG - chr3_1:43566314-43566333 Msa0358540:CDS 25.0%
! TTTGAAGGTTTGTATTTCTT+TGG - chr3_1:43566429-43566448 Msa0358540:intron 25.0%
!! AAAGTGACTTGTGAAATATT+TGG - chr3_1:43566752-43566771 Msa0358540:intron 25.0%
!! TAGTTTCTGTGTTGAATTTT+TGG + chr3_1:43567236-43567255 None:intergenic 25.0%
! GACAAAGGTAAAAAAGAAAA+AGG + chr3_1:43567302-43567321 None:intergenic 25.0%
!!! TTCTTTTTTACCTTTGTCAA+AGG - chr3_1:43567304-43567323 Msa0358540:intron 25.0%
! ATAATGAATGATTACCAATG+TGG + chr3_1:43567380-43567399 None:intergenic 25.0%
!!! ATTAAAATTGTTTTGTGTGG+GGG + chr3_1:43567406-43567425 None:intergenic 25.0%
!!! TTTGAATTGGTTTTAGGATA+TGG - chr3_1:43567442-43567461 Msa0358540:CDS 25.0%
!!! TTGAATTGGTTTTAGGATAT+GGG - chr3_1:43567443-43567462 Msa0358540:CDS 25.0%
! AATAAGTATTATATGACTGC+AGG + chr3_1:43567589-43567608 None:intergenic 25.0%
!! GATGAATAAATGGAGAATTT+TGG + chr3_1:43567635-43567654 None:intergenic 25.0%
! TTCTCCATTTATTCATCTTA+TGG - chr3_1:43567638-43567657 Msa0358540:intron 25.0%
! TCTCCATTTATTCATCTTAT+GGG - chr3_1:43567639-43567658 Msa0358540:intron 25.0%
! TATCCCATAAGATGAATAAA+TGG + chr3_1:43567645-43567664 None:intergenic 25.0%
!! CATCTTATGGGATAAAATTT+TGG - chr3_1:43567651-43567670 Msa0358540:intron 25.0%
!!! AACAATGTTTAGCTTTTGTT+TGG - chr3_1:43568173-43568192 Msa0358540:CDS 25.0%
! TTTGATATTGTAACAATGAC+AGG - chr3_1:43568222-43568241 Msa0358540:CDS 25.0%
TGATGTTTGCAATCAAAGAT+TGG - chr3_1:43566068-43566087 Msa0358540:intron 30.0%
GATGTTTGCAATCAAAGATT+GGG - chr3_1:43566069-43566088 Msa0358540:intron 30.0%
TTAATAGAACCATACCCTTT+CGG + chr3_1:43566137-43566156 None:intergenic 30.0%
AACTATTATGTGCAGCATTT+GGG + chr3_1:43566508-43566527 None:intergenic 30.0%
AACAAATATGTGCAGCAATT+GGG + chr3_1:43566567-43566586 None:intergenic 30.0%
AAAGAAGAAGACTAAACTTG+AGG - chr3_1:43566600-43566619 Msa0358540:intron 30.0%
AAGAAGAAGACTAAACTTGA+GGG - chr3_1:43566601-43566620 Msa0358540:intron 30.0%
!!! GACTCTTAGAAGCTTTTTTT+TGG - chr3_1:43566774-43566793 Msa0358540:intron 30.0%
! CTCTCTAATGTGAAGTTTTT+AGG - chr3_1:43566820-43566839 Msa0358540:intron 30.0%
!!! CTAATGTGAAGTTTTTAGGT+AGG - chr3_1:43566824-43566843 Msa0358540:intron 30.0%
GAATTGTATGTGAATAGCTA+TGG - chr3_1:43566888-43566907 Msa0358540:intron 30.0%
!!! TTTTTGTTGCTTTCAGGTAT+GGG - chr3_1:43566911-43566930 Msa0358540:intron 30.0%
TGTGGAGAAGATAAAATGTT+AGG - chr3_1:43566944-43566963 Msa0358540:intron 30.0%
!! TTTTCATGTTGCTTGAATTG+AGG - chr3_1:43567013-43567032 Msa0358540:intron 30.0%
ACTATGCCAAATATGAATCA+TGG + chr3_1:43567180-43567199 None:intergenic 30.0%
CAATCATATACCTTTGACAA+AGG + chr3_1:43567317-43567336 None:intergenic 30.0%
CCAATTCAAACAAATACTTG+AGG + chr3_1:43567432-43567451 None:intergenic 30.0%
CCTCAAGTATTTGTTTGAAT+TGG - chr3_1:43567429-43567448 Msa0358540:CDS 30.0%
TACCAGAACCTTATCATAAT+TGG - chr3_1:43567548-43567567 Msa0358540:intron 30.0%
AACCAATTATGATAAGGTTC+TGG + chr3_1:43567553-43567572 None:intergenic 30.0%
GAACCTTATCATAATTGGTT+TGG - chr3_1:43567553-43567572 Msa0358540:intron 30.0%
! AGAATTTTGGATTCAAACCT+CGG + chr3_1:43567622-43567641 None:intergenic 30.0%
!! TTTTTAGTCAAGTAGTCCAA+TGG + chr3_1:43567677-43567696 None:intergenic 30.0%
!!! TCATTGTTTTTGCAGTGTAT+CGG + chr3_1:43567923-43567942 None:intergenic 30.0%
CACTGCAAAAACAATGATTA+AGG - chr3_1:43567926-43567945 Msa0358540:intron 30.0%
! ATTTTATGTCGAGTATGTCA+AGG - chr3_1:43567964-43567983 Msa0358540:intron 30.0%
TGAATGTATTTGGCTAAGAA+TGG - chr3_1:43568023-43568042 Msa0358540:intron 30.0%
ACTATCCCTATACAAGATTT+GGG + chr3_1:43568105-43568124 None:intergenic 30.0%
AACTATCCCTATACAAGATT+TGG + chr3_1:43568106-43568125 None:intergenic 30.0%
ATCTTGTATAGGGATAGTTT+AGG - chr3_1:43568107-43568126 Msa0358540:CDS 30.0%
AAGCATAGTTACTAACATAG+AGG + chr3_1:43568150-43568169 None:intergenic 30.0%
! GTAGTTTTTCGTCAGATCTT+GGG - chr3_1:43565619-43565638 Msa0358540:three_prime_UTR 35.0%
GTGATTAATCCTCCTTCATT+CGG - chr3_1:43565796-43565815 Msa0358540:three_prime_UTR 35.0%
ACAAAAAATCCGAATGAAGG+AGG + chr3_1:43565808-43565827 None:intergenic 35.0%
TGGACAAAAAATCCGAATGA+AGG + chr3_1:43565811-43565830 None:intergenic 35.0%
ATAAACAAAAAACGTACCGG+AGG + chr3_1:43566020-43566039 None:intergenic 35.0%
! GAAGATTCTTGACGTTTTTG+TGG - chr3_1:43566097-43566116 Msa0358540:intron 35.0%
TTCGTATCAAAAACCCGAAA+GGG - chr3_1:43566120-43566139 Msa0358540:intron 35.0%
TAATAGAACCATACCCTTTC+GGG + chr3_1:43566136-43566155 None:intergenic 35.0%
! ATGGTTCTATTAAACCCGAA+AGG - chr3_1:43566144-43566163 Msa0358540:intron 35.0%
! TGGTTCTATTAAACCCGAAA+GGG - chr3_1:43566145-43566164 Msa0358540:intron 35.0%
CAACTATTATGTGCAGCATT+TGG + chr3_1:43566509-43566528 None:intergenic 35.0%
CAACAAATATGTGCAGCAAT+TGG + chr3_1:43566568-43566587 None:intergenic 35.0%
!!! TGTGAAGTTTTTAGGTAGGT+TGG - chr3_1:43566828-43566847 Msa0358540:intron 35.0%
!!! CTATGGTTTTTGTTGCTTTC+AGG - chr3_1:43566905-43566924 Msa0358540:intron 35.0%
!!! GTTTTTGTTGCTTTCAGGTA+TGG - chr3_1:43566910-43566929 Msa0358540:intron 35.0%
! GCTTGAATTGAGGTTCTTTT+TGG - chr3_1:43567023-43567042 Msa0358540:intron 35.0%
!! TTGGTGCCATGATTCATATT+TGG - chr3_1:43567171-43567190 Msa0358540:intron 35.0%
ATTATGTATCATGAGTGCCA+AGG - chr3_1:43567206-43567225 Msa0358540:intron 35.0%
TTATGTATCATGAGTGCCAA+GGG - chr3_1:43567207-43567226 Msa0358540:intron 35.0%
ATTAGTCCCAACCATAATGT+TGG - chr3_1:43567342-43567361 Msa0358540:intron 35.0%
TTAGTCCCAACCATAATGTT+GGG - chr3_1:43567343-43567362 Msa0358540:intron 35.0%
AATGTCCCAACATTATGGTT+GGG + chr3_1:43567351-43567370 None:intergenic 35.0%
TAATGTCCCAACATTATGGT+TGG + chr3_1:43567352-43567371 None:intergenic 35.0%
CCATTAATGTCCCAACATTA+TGG + chr3_1:43567356-43567375 None:intergenic 35.0%
CCATAATGTTGGGACATTAA+TGG - chr3_1:43567353-43567372 Msa0358540:intron 35.0%
GCACCAAACCAATTATGATA+AGG + chr3_1:43567559-43567578 None:intergenic 35.0%
!! TGGGATAAAATTTTGGCCAT+TGG - chr3_1:43567658-43567677 Msa0358540:intron 35.0%
TAGATTAATCGGAAACATGC+AGG - chr3_1:43567817-43567836 Msa0358540:intron 35.0%
AGTATGTCAAGGCTTCAAAT+TGG - chr3_1:43567975-43567994 Msa0358540:intron 35.0%
ATACAAGATTTGGGGAAGAA+AGG + chr3_1:43568096-43568115 None:intergenic 35.0%
TTCTTCCCCAAATCTTGTAT+AGG - chr3_1:43568096-43568115 Msa0358540:CDS 35.0%
TCTTCCCCAAATCTTGTATA+GGG - chr3_1:43568097-43568116 Msa0358540:CDS 35.0%
CTATCCCTATACAAGATTTG+GGG + chr3_1:43568104-43568123 None:intergenic 35.0%
! GGTAGTTTTTCGTCAGATCT+TGG - chr3_1:43565618-43565637 Msa0358540:three_prime_UTR 40.0%
CAGAACCAACAAACAGACAT+CGG + chr3_1:43565647-43565666 None:intergenic 40.0%
!! TCTGTTTGTTGGTTCTGGTA+AGG - chr3_1:43565650-43565669 Msa0358540:three_prime_UTR 40.0%
!! CTGTTTGTTGGTTCTGGTAA+GGG - chr3_1:43565651-43565670 Msa0358540:three_prime_UTR 40.0%
!!! CTTCTTCTGCAGGTTTTGTT+TGG - chr3_1:43565750-43565769 Msa0358540:three_prime_UTR 40.0%
AGGAGGATTAATCACAGTGA+AGG + chr3_1:43565791-43565810 None:intergenic 40.0%
GTTTGCAATCAAAGATTGGG+CGG - chr3_1:43566072-43566091 Msa0358540:intron 40.0%
GTTCGTATCAAAAACCCGAA+AGG - chr3_1:43566119-43566138 Msa0358540:intron 40.0%
ATCAAAAACCCGAAAGGGTA+TGG - chr3_1:43566125-43566144 Msa0358540:intron 40.0%
GTCCTTGTCGGTAAAAACAT+CGG + chr3_1:43566395-43566414 None:intergenic 40.0%
CTTGAAAGAGAAGGCTCATT+TGG - chr3_1:43566629-43566648 Msa0358540:intron 40.0%
AGTAGCACATGATCACTTTG+AGG - chr3_1:43566713-43566732 Msa0358540:intron 40.0%
! CACAAGTCACTTTGCATTTC+AGG + chr3_1:43566745-43566764 None:intergenic 40.0%
CTGTTTGAACAGTTGTGTCT+TGG + chr3_1:43566983-43567002 None:intergenic 40.0%
CAAGACACAACTGTTCAAAC+AGG - chr3_1:43566981-43567000 Msa0358540:intron 40.0%
TTGAATGTGATTGCACGTGT+CGG + chr3_1:43567093-43567112 None:intergenic 40.0%
! TCAGTGTCGTGTATTTCTGT+TGG - chr3_1:43567152-43567171 Msa0358540:intron 40.0%
TATGTATCATGAGTGCCAAG+GGG - chr3_1:43567208-43567227 Msa0358540:intron 40.0%
CACTATGGACTTCACTTGTA+AGG + chr3_1:43567508-43567527 None:intergenic 40.0%
!! AGAGCTTGTTGATGAAACAC+AGG - chr3_1:43567840-43567859 Msa0358540:intron 40.0%
TATTTGGCTAAGAATGGCCA+AGG - chr3_1:43568029-43568048 Msa0358540:intron 40.0%
AAGATTTGGGGAAGAAAGGA+AGG + chr3_1:43568092-43568111 None:intergenic 40.0%
AAGAAAGAAGCAACGACGAC+CGG + chr3_1:43565563-43565582 None:intergenic 45.0%
AAACTACCAAACCTCGAAGC+TGG + chr3_1:43565606-43565625 None:intergenic 45.0%
!! CGATGTCTGTTTGTTGGTTC+TGG - chr3_1:43565645-43565664 Msa0358540:three_prime_UTR 45.0%
ACAACCTGGAACACAAGTGA+CGG + chr3_1:43565700-43565719 None:intergenic 45.0%
TCACTTGTGTTCCAGGTTGT+GGG - chr3_1:43565700-43565719 Msa0358540:three_prime_UTR 45.0%
!!! TTTTTTGTCCAGTCGTCGCA+AGG - chr3_1:43565820-43565839 Msa0358540:three_prime_UTR 45.0%
AATGGTCATGGATCTGCTAC+AGG - chr3_1:43565869-43565888 Msa0358540:three_prime_UTR 45.0%
ATGGATCTGCTACAGGTTGT+TGG - chr3_1:43565876-43565895 Msa0358540:exon 45.0%
! TATTGTCGGTTGTGTTCCGT+CGG - chr3_1:43565951-43565970 Msa0358540:CDS 45.0%
AACAAACATAAGCCAGTCGG+CGG + chr3_1:43565992-43566011 None:intergenic 45.0%
GCCAACAAACATAAGCCAGT+CGG + chr3_1:43565995-43566014 None:intergenic 45.0%
!! GACTGGCTTATGTTTGTTGG+CGG - chr3_1:43565994-43566013 Msa0358540:intron 45.0%
GAACCCATAAGAGATCCCAT+GGG - chr3_1:43566336-43566355 Msa0358540:intron 45.0%
TAGCCCATGGGATCTCTTAT+GGG + chr3_1:43566342-43566361 None:intergenic 45.0%
CTTGTCGGTAAAAACATCGG+TGG + chr3_1:43566392-43566411 None:intergenic 45.0%
!! CACCGATGTTTTTACCGACA+AGG - chr3_1:43566390-43566409 Msa0358540:intron 45.0%
CAAAGCTGCTGCTCATTTGA+AGG - chr3_1:43566414-43566433 Msa0358540:intron 45.0%
AGGGAGAGTCTTGAAAGAGA+AGG - chr3_1:43566620-43566639 Msa0358540:intron 45.0%
GGTATGGGACTTGAAAAGTG+TGG - chr3_1:43566926-43566945 Msa0358540:intron 45.0%
!!! GTTGAATTTTTGGCTCCCCT+TGG + chr3_1:43567226-43567245 None:intergenic 45.0%
GGGACATTAATGGTCCACAT+TGG - chr3_1:43567363-43567382 Msa0358540:intron 45.0%
CCTCTTGCTTCATAGCACTA+TGG + chr3_1:43567523-43567542 None:intergenic 45.0%
CCATAGTGCTATGAAGCAAG+AGG - chr3_1:43567520-43567539 Msa0358540:intron 45.0%
TTGCCATGCACTCTTCTCAT+TGG - chr3_1:43568054-43568073 Msa0358540:intron 45.0%
AGAAAGAAGCAACGACGACC+GGG + chr3_1:43565562-43565581 None:intergenic 50.0%
CTGGCGGAGAGATGTTTATC+TGG + chr3_1:43565587-43565606 None:intergenic 50.0%
GGGTTCCGATGTCTGTTTGT+TGG - chr3_1:43565639-43565658 Msa0358540:three_prime_UTR 50.0%
!! TGTTGGTTCTGGTAAGGGAG+CGG - chr3_1:43565656-43565675 Msa0358540:three_prime_UTR 50.0%
GTCACTTGTGTTCCAGGTTG+TGG - chr3_1:43565699-43565718 Msa0358540:three_prime_UTR 50.0%
TGGATCTACAACCCACAACC+TGG + chr3_1:43565714-43565733 None:intergenic 50.0%
GAAGTCGACAGAACTGGATC+TGG + chr3_1:43565734-43565753 None:intergenic 50.0%
GCAGAAGAAGTCGACAGAAC+TGG + chr3_1:43565740-43565759 None:intergenic 50.0%
!! GTTCTGTCGACTTCTTCTGC+AGG - chr3_1:43565740-43565759 Msa0358540:three_prime_UTR 50.0%
! GGGTGGTTCTGTAATGGTCA+TGG - chr3_1:43565857-43565876 Msa0358540:three_prime_UTR 50.0%
GATCTGCTACAGGTTGTTGG+CGG - chr3_1:43565879-43565898 Msa0358540:exon 50.0%
GCTAAACAAACGGCAACCGA+CGG + chr3_1:43565970-43565989 None:intergenic 50.0%
!! GCCGACTGGCTTATGTTTGT+TGG - chr3_1:43565991-43566010 Msa0358540:intron 50.0%
! TTATGTTTGTTGGCGGCCTC+CGG - chr3_1:43566001-43566020 Msa0358540:intron 50.0%
GGAACCCATAAGAGATCCCA+TGG - chr3_1:43566335-43566354 Msa0358540:intron 50.0%
CTAGCCCATGGGATCTCTTA+TGG + chr3_1:43566343-43566362 None:intergenic 50.0%
! AGCAGCAGCTTTGTCCTTGT+CGG + chr3_1:43566407-43566426 None:intergenic 50.0%
ACTTATTGACATAGGGGCCG+AGG - chr3_1:43567602-43567621 Msa0358540:intron 50.0%
! AAGAGTGCATGGCAAGACCT+TGG + chr3_1:43568049-43568068 None:intergenic 50.0%
! CCACCAATGAGAAGAGTGCA+TGG + chr3_1:43568060-43568079 None:intergenic 50.0%
CCATGCACTCTTCTCATTGG+TGG - chr3_1:43568057-43568076 Msa0358540:intron 50.0%
GAAAGAAGCAACGACGACCG+GGG + chr3_1:43565561-43565580 None:intergenic 55.0%
CTACCAAACCTCGAAGCTGG+CGG + chr3_1:43565603-43565622 None:intergenic 55.0%
! GGGTGAGGGTGGTTCTGTAA+TGG - chr3_1:43565851-43565870 Msa0358540:three_prime_UTR 55.0%
GCTACAGGTTGTTGGCGGTT+TGG - chr3_1:43565884-43565903 Msa0358540:exon 55.0%
! ACAGGTTGTTGGCGGTTTGG+TGG - chr3_1:43565887-43565906 Msa0358540:exon 55.0%
! GTTTGGTGGCAGCCTCTTCA+CGG - chr3_1:43565901-43565920 Msa0358540:CDS 55.0%
CCAGTCGGCGGCTAAACAAA+CGG + chr3_1:43565980-43565999 None:intergenic 55.0%
CGACACTGACATGTCGACAC+CGG + chr3_1:43567141-43567160 None:intergenic 55.0%
AACGACGACCGGGGAAAAGG+GGG + chr3_1:43565552-43565571 None:intergenic 60.0%
CAACGACGACCGGGGAAAAG+GGG + chr3_1:43565553-43565572 None:intergenic 60.0%
GCAACGACGACCGGGGAAAA+GGG + chr3_1:43565554-43565573 None:intergenic 60.0%
AGCAACGACGACCGGGGAAA+AGG + chr3_1:43565555-43565574 None:intergenic 60.0%
ACATCTCTCCGCCAGCTTCG+AGG - chr3_1:43565592-43565611 Msa0358540:three_prime_UTR 60.0%
TCTCCGCCAGCTTCGAGGTT+TGG - chr3_1:43565597-43565616 Msa0358540:three_prime_UTR 60.0%
!! TGGTTCTGGTAAGGGAGCGG+CGG - chr3_1:43565659-43565678 Msa0358540:three_prime_UTR 60.0%
TCCAGTCGTCGCAAGGCTCA+TGG - chr3_1:43565827-43565846 Msa0358540:three_prime_UTR 60.0%
ACCATGAGCCTTGCGACGAC+TGG + chr3_1:43565831-43565850 None:intergenic 60.0%
AGTCGTCGCAAGGCTCATGG+TGG - chr3_1:43565830-43565849 Msa0358540:three_prime_UTR 60.0%
GTCGTCGCAAGGCTCATGGT+GGG - chr3_1:43565831-43565850 Msa0358540:three_prime_UTR 60.0%
!! GCAAGGCTCATGGTGGGTGA+GGG - chr3_1:43565837-43565856 Msa0358540:three_prime_UTR 60.0%
ACACAATCGCCGCCGTGAAG+AGG + chr3_1:43565916-43565935 None:intergenic 60.0%
CCGTTTGTTTAGCCGCCGAC+TGG - chr3_1:43565977-43565996 Msa0358540:intron 60.0%
!! GAGATCCCATGGGCTAGCAC+TGG - chr3_1:43566346-43566365 Msa0358540:intron 60.0%
CAGCACCAGTGCTAGCCCAT+GGG + chr3_1:43566354-43566373 None:intergenic 60.0%
TCAGCACCAGTGCTAGCCCA+TGG + chr3_1:43566355-43566374 None:intergenic 60.0%
GGCGCCGTCACTTGTGTTCC+AGG - chr3_1:43565693-43565712 Msa0358540:three_prime_UTR 65.0%
!! CGCAAGGCTCATGGTGGGTG+AGG - chr3_1:43565836-43565855 Msa0358540:three_prime_UTR 65.0%
!! AGGCTCATGGTGGGTGAGGG+TGG - chr3_1:43565840-43565859 Msa0358540:three_prime_UTR 65.0%
TGGTGGCAGCCTCTTCACGG+CGG - chr3_1:43565904-43565923 Msa0358540:CDS 65.0%
AAAAGGGGGCGGCGGTGGTG+CGG + chr3_1:43565538-43565557 None:intergenic 70.0%
GACGACCGGGGAAAAGGGGG+CGG + chr3_1:43565549-43565568 None:intergenic 70.0%
! CACCGCCGCCCCCTTTTCCC+CGG - chr3_1:43565541-43565560 Msa0358540:three_prime_UTR 75.0%
GACCGGGGAAAAGGGGGCGG+CGG + chr3_1:43565546-43565565 None:intergenic 75.0%
GGTAAGGGAGCGGCGGCGCT+TGG - chr3_1:43565666-43565685 Msa0358540:three_prime_UTR 75.0%
AAGGGAGCGGCGGCGCTTGG+TGG - chr3_1:43565669-43565688 Msa0358540:three_prime_UTR 75.0%
! CGGGGAAAAGGGGGCGGCGG+TGG + chr3_1:43565543-43565562 None:intergenic 80.0%
!! GGAGCGGCGGCGCTTGGTGG+TGG - chr3_1:43565672-43565691 Msa0358540:three_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 43565522 43568287 43565522 ID=Msa0358540;Name=Msa0358540
chr3_1 mRNA 43565522 43568287 43565522 ID=Msa0358540-mRNA-1;Parent=Msa0358540;Name=Msa0358540-mRNA-1;_AED=0.28;_eAED=0.44;_QI=0|0.33|0.5|1|1|1|4|369|154
chr3_1 exon 43565522 43565989 43565522 ID=Msa0358540-mRNA-1:exon:7753;Parent=Msa0358540-mRNA-1
chr3_1 exon 43566268 43566351 43566268 ID=Msa0358540-mRNA-1:exon:7752;Parent=Msa0358540-mRNA-1
chr3_1 exon 43567374 43567462 43567374 ID=Msa0358540-mRNA-1:exon:7751;Parent=Msa0358540-mRNA-1
chr3_1 exon 43568095 43568287 43568095 ID=Msa0358540-mRNA-1:exon:7750;Parent=Msa0358540-mRNA-1
chr3_1 CDS 43568095 43568287 43568095 ID=Msa0358540-mRNA-1:cds;Parent=Msa0358540-mRNA-1
chr3_1 CDS 43567374 43567462 43567374 ID=Msa0358540-mRNA-1:cds;Parent=Msa0358540-mRNA-1
chr3_1 CDS 43566268 43566351 43566268 ID=Msa0358540-mRNA-1:cds;Parent=Msa0358540-mRNA-1
chr3_1 CDS 43565891 43565989 43565891 ID=Msa0358540-mRNA-1:cds;Parent=Msa0358540-mRNA-1
chr3_1 three_prime_UTR 43565522 43565890 43565522 ID=Msa0358540-mRNA-1:three_prime_utr;Parent=Msa0358540-mRNA-1
Gene Sequence

>Msa0358540

ATGGGTTCTTTCTCCGCACCACCGCCGCCCCCTTTTCCCCGGTCGTCGTTGCTTCTTTCTTTCCAGATAAACATCTCTCCGCCAGCTTCGAGGTTTGGTAGTTTTTCGTCAGATCTTGGGTTCCGATGTCTGTTTGTTGGTTCTGGTAAGGGAGCGGCGGCGCTTGGTGGTGGCGCCGTCACTTGTGTTCCAGAGATCCCATGGGCTAGCACTGGTGCTGATATTATTGTTGAGTCCACCGATGTTTTTACCGACAAGGACAAAGCTGCTGCTCATTTGAAGGATATGGGCTACGTCACTGAATGTGCTAAATCAAGCAATAAACAAAGCCTTACAAGTGAAGTCCATAGTGCTATGAAGCAAGAGATTAATCGGAAACATGCAGGAGAGCTTGTTGATGAAACACAGGTGAACATCGCATTGAATTTCTACTATGAGATTGTAGAAAGTACAAAAAAAATATGA
Protein sequence

>Msa0358540

MGSFSAPPPPPFPRSSLLLSFQINISPPASRFGSFSSDLGFRCLFVGSGKGAAALGGGAVTCVPEIPWASTGADIIVESTDVFTDKDKAAAHLKDMGYVTECAKSSNKQSLTSEVHSAMKQEINRKHAGELVDETQVNIALNFYYEIVESTKKI*