AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0367070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0367070 MtrunA17_Chr3g0107931 62.762 239 40 3 19 208 1227 1465 3.28e-82 266
Msa0367070 MtrunA17_Chr3g0107271 56.299 254 56 6 16 220 2 249 1.89e-81 243
Msa0367070 MtrunA17_Chr3g0108111 56.198 242 57 3 18 210 149 390 1.43e-75 239
Msa0367070 MtrunA17_Chr3g0108151 56.790 243 54 4 22 213 975 1217 4.62e-75 246
Msa0367070 MtrunA17_Chr3g0108821 55.085 236 56 5 25 212 1204 1437 7.86e-69 228
Msa0367070 MtrunA17_Chr3g0091861 52.245 245 63 3 22 212 1152 1396 1.83e-68 227
Msa0367070 MtrunA17_Chr3g0108071 50.391 256 73 4 15 220 345 596 3.04e-67 222
Msa0367070 MtrunA17_Chr3g0108171 69.811 159 42 1 68 220 1218 1376 2.73e-65 218
Msa0367070 MtrunA17_Chr3g0108011 54.626 227 45 3 25 208 1242 1453 3.75e-64 215
Msa0367070 MtrunA17_Chr3g0108811 68.553 159 41 3 64 213 1418 1576 1.43e-61 207
Msa0367070 MtrunA17_Chr3g0108811 47.305 167 42 3 25 147 1225 1389 5.12e-36 134
Msa0367070 MtrunA17_Chr3g0097601 47.581 248 70 5 22 215 9 250 6.41e-60 189
Msa0367070 MtrunA17_Chr2g0288311 63.291 158 32 3 67 220 43 178 2.31e-56 177
Msa0367070 MtrunA17_Chr3g0107081 47.826 207 86 6 22 220 12 204 9.20e-55 174
Msa0367070 MtrunA17_Chr3g0096701 60.690 145 51 1 66 204 63 207 5.50e-50 173
Msa0367070 MtrunA17_Chr3g0108091 64.885 131 39 1 97 220 3 133 8.79e-50 169
Msa0367070 MtrunA17_Chr3g0105481 56.494 154 57 3 67 211 1115 1267 7.25e-49 171
Msa0367070 MtrunA17_Chr3g0105601 52.174 161 65 3 68 219 322 479 3.25e-45 157
Msa0367070 MtrunA17_Chr3g0105551 44.976 209 63 3 50 206 1141 1349 2.03e-44 158
Msa0367070 MtrunA17_Chr3g0105521 56.338 142 53 2 68 200 1084 1225 8.12e-42 150
Msa0367070 MtrunA17_Chr3g0105501 56.391 133 50 3 67 195 54 182 9.15e-40 135
Msa0367070 MtrunA17_Chr3g0096801 65.517 87 30 0 114 200 2 88 2.77e-34 119
Msa0367070 MtrunA17_Chr3g0107201 61.321 106 30 2 114 209 2 106 1.16e-30 118
Msa0367070 MtrunA17_Chr1g0160951 28.235 255 125 7 21 219 998 1250 3.57e-23 97.4
Msa0367070 MtrunA17_Chr3g0100241 36.885 122 69 3 68 181 69 190 2.41e-17 77.4
Msa0367070 MtrunA17_Chr5g0439121 33.333 150 90 5 68 207 1376 1525 1.77e-16 77.8
Msa0367070 MtrunA17_Chr3g0100261 36.290 124 69 3 68 181 1085 1208 6.57e-16 76.3
Msa0367070 MtrunA17_Chr8g0360991 30.534 131 74 2 68 196 1079 1194 2.83e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAACAGGAAATGAATTT+AGG 0.211262 3_1:-62586714 Msa0367070:CDS
AATACTACAAATTGTAGATT+TGG 0.253439 3_1:+62586334 None:intergenic
GCATATTATTGAAGATGATT+TGG 0.296423 3_1:-62586551 Msa0367070:CDS
CTCAGGAAGCTCTTTACATA+TGG 0.356714 3_1:+62586438 None:intergenic
ACAACAAGTATGTTTAACTT+TGG 0.362700 3_1:+62586499 None:intergenic
CAACAAGTATGTTTAACTTT+GGG 0.367815 3_1:+62586500 None:intergenic
TATCACTTTGAAAAGACTTC+AGG 0.379137 3_1:-62586781 Msa0367070:CDS
CTTTGGGAAGCATGTCTTTG+AGG 0.405904 3_1:+62586516 None:intergenic
GATAAGAGAAGCAGATGAGT+TGG 0.410082 3_1:-62586401 Msa0367070:CDS
TCTCAATGAAATCAATGAAC+AGG 0.412162 3_1:-62586727 Msa0367070:CDS
AGCTCTTTACATATGGAGAT+TGG 0.415026 3_1:+62586445 None:intergenic
CAGTTTGAAAATGAATTCTC+TGG 0.417455 3_1:+62586284 None:intergenic
TTTGGGAAGCATGTCTTTGA+GGG 0.418532 3_1:+62586517 None:intergenic
TTTCTGTGCAGGAATTGATT+GGG 0.458993 3_1:-62586818 Msa0367070:intron
GAATTGTCAAACACTATCTC+TGG 0.469868 3_1:-62586242 Msa0367070:CDS
ATCACTAGACACTTTAGCTC+AGG 0.476998 3_1:+62586421 None:intergenic
CTCTGGACATGACTGAGGCT+TGG 0.501155 3_1:+62586301 None:intergenic
AAGAACAATAGATCTTGATC+AGG 0.506672 3_1:-62587275 Msa0367070:CDS
GATGAAATATTTGCAAATGA+AGG 0.527514 3_1:-62586379 Msa0367070:CDS
AGATCTTGATCAGGAAACTT+CGG 0.543596 3_1:-62587266 Msa0367070:CDS
GATCGTTTAGAGTTTGGCTA+CGG 0.557476 3_1:-62586184 Msa0367070:CDS
GCAGGAATTGATTGGGAATG+TGG 0.595247 3_1:-62586811 Msa0367070:intron
GAATTCTCTGGACATGACTG+AGG 0.634620 3_1:+62586296 None:intergenic
ATTCTCATATAAAGCAACTG+TGG 0.637426 3_1:+62586595 None:intergenic
TGAAGGTGATGATCACAAAG+TGG 0.652737 3_1:-62586362 Msa0367070:CDS
TTCGGAAAGCAGAATCGAAG+CGG 0.677128 3_1:-62587248 Msa0367070:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATCTATTACTTTTATTAA+AGG - chr3_1:62586475-62586494 Msa0367070:CDS 10.0%
!!! AATCTATTACTTTTATTAAA+GGG - chr3_1:62586476-62586495 Msa0367070:CDS 10.0%
!!! TAATCTATTACTTTTATTAA+AGG - chr3_1:62586475-62586494 Msa0367070:CDS 10.0%
!!! AATCTATTACTTTTATTAAA+GGG - chr3_1:62586476-62586495 Msa0367070:CDS 10.0%
!! ATTCATTGTAATAAATCATT+TGG + chr3_1:62586293-62586312 None:intergenic 15.0%
!!! GTTAAAGTTTTTAAATGATT+TGG - chr3_1:62586345-62586364 Msa0367070:CDS 15.0%
!!! ACTTTGTTATTCTTATTTTA+TGG - chr3_1:62586589-62586608 Msa0367070:CDS 15.0%
!!! ATTTTCAATTTTTCACAAAA+AGG + chr3_1:62586802-62586821 None:intergenic 15.0%
!! ATTCATTGTAATAAATCATT+TGG + chr3_1:62586293-62586312 None:intergenic 15.0%
!!! GTTAAAGTTTTTAAATGATT+TGG - chr3_1:62586345-62586364 Msa0367070:CDS 15.0%
!!! ACTTTGTTATTCTTATTTTA+TGG - chr3_1:62586589-62586608 Msa0367070:CDS 15.0%
!!! ATTTTCAATTTTTCACAAAA+AGG + chr3_1:62586802-62586821 None:intergenic 15.0%
!! AATACTACAAATTGTAGATT+TGG + chr3_1:62587117-62587136 None:intergenic 20.0%
!! AATACTACAAATTGTAGATT+TGG + chr3_1:62587117-62587136 None:intergenic 20.0%
! AAAATTAGACAGATGTTCAA+GGG + chr3_1:62586442-62586461 None:intergenic 25.0%
! TAAAATTAGACAGATGTTCA+AGG + chr3_1:62586443-62586462 None:intergenic 25.0%
!!! TTACTTTTATTAAAGGGTAG+CGG - chr3_1:62586482-62586501 Msa0367070:CDS 25.0%
! AATGAACAGGAAATGAATTT+AGG - chr3_1:62586734-62586753 Msa0367070:CDS 25.0%
!! TTTTCTCCACTTCTATTATA+AGG - chr3_1:62586824-62586843 Msa0367070:intron 25.0%
! GCATATTATTGAAGATGATT+TGG - chr3_1:62586897-62586916 Msa0367070:intron 25.0%
!! CAACAAGTATGTTTAACTTT+GGG + chr3_1:62586951-62586970 None:intergenic 25.0%
!! ACAACAAGTATGTTTAACTT+TGG + chr3_1:62586952-62586971 None:intergenic 25.0%
! GATGAAATATTTGCAAATGA+AGG - chr3_1:62587069-62587088 Msa0367070:intron 25.0%
! AAAATTAGACAGATGTTCAA+GGG + chr3_1:62586442-62586461 None:intergenic 25.0%
! TAAAATTAGACAGATGTTCA+AGG + chr3_1:62586443-62586462 None:intergenic 25.0%
!!! TTACTTTTATTAAAGGGTAG+CGG - chr3_1:62586482-62586501 Msa0367070:CDS 25.0%
! AATGAACAGGAAATGAATTT+AGG - chr3_1:62586734-62586753 Msa0367070:CDS 25.0%
!! TTTTCTCCACTTCTATTATA+AGG - chr3_1:62586824-62586843 Msa0367070:intron 25.0%
! GCATATTATTGAAGATGATT+TGG - chr3_1:62586897-62586916 Msa0367070:intron 25.0%
!! CAACAAGTATGTTTAACTTT+GGG + chr3_1:62586951-62586970 None:intergenic 25.0%
!! ACAACAAGTATGTTTAACTT+TGG + chr3_1:62586952-62586971 None:intergenic 25.0%
! GATGAAATATTTGCAAATGA+AGG - chr3_1:62587069-62587088 Msa0367070:intron 25.0%
AAGAACAATAGATCTTGATC+AGG - chr3_1:62586173-62586192 Msa0367070:CDS 30.0%
GCATAAATACTTTGATGGAA+TGG + chr3_1:62586244-62586263 None:intergenic 30.0%
ATGCTAGTAGCTACTATATA+TGG + chr3_1:62586375-62586394 None:intergenic 30.0%
ATAGTAGCTACTAGCATTTA+AGG - chr3_1:62586378-62586397 Msa0367070:CDS 30.0%
TAGTAGCTACTAGCATTTAA+GGG - chr3_1:62586379-62586398 Msa0367070:CDS 30.0%
! TATCACTTTGAAAAGACTTC+AGG - chr3_1:62586667-62586686 Msa0367070:intron 30.0%
TCTCAATGAAATCAATGAAC+AGG - chr3_1:62586721-62586740 Msa0367070:CDS 30.0%
TAGACACCTTATAATAGAAG+TGG + chr3_1:62586833-62586852 None:intergenic 30.0%
ATTCTCATATAAAGCAACTG+TGG + chr3_1:62586856-62586875 None:intergenic 30.0%
CAGTTTGAAAATGAATTCTC+TGG + chr3_1:62587167-62587186 None:intergenic 30.0%
! AACGATCAAAATCATTTTCG+AGG + chr3_1:62587251-62587270 None:intergenic 30.0%
!! GATTTTGATCGTTTAGAGTT+TGG - chr3_1:62587258-62587277 Msa0367070:CDS 30.0%
AAGAACAATAGATCTTGATC+AGG - chr3_1:62586173-62586192 Msa0367070:CDS 30.0%
GCATAAATACTTTGATGGAA+TGG + chr3_1:62586244-62586263 None:intergenic 30.0%
ATGCTAGTAGCTACTATATA+TGG + chr3_1:62586375-62586394 None:intergenic 30.0%
ATAGTAGCTACTAGCATTTA+AGG - chr3_1:62586378-62586397 Msa0367070:CDS 30.0%
TAGTAGCTACTAGCATTTAA+GGG - chr3_1:62586379-62586398 Msa0367070:CDS 30.0%
! TATCACTTTGAAAAGACTTC+AGG - chr3_1:62586667-62586686 Msa0367070:intron 30.0%
TCTCAATGAAATCAATGAAC+AGG - chr3_1:62586721-62586740 Msa0367070:CDS 30.0%
TAGACACCTTATAATAGAAG+TGG + chr3_1:62586833-62586852 None:intergenic 30.0%
ATTCTCATATAAAGCAACTG+TGG + chr3_1:62586856-62586875 None:intergenic 30.0%
CAGTTTGAAAATGAATTCTC+TGG + chr3_1:62587167-62587186 None:intergenic 30.0%
! AACGATCAAAATCATTTTCG+AGG + chr3_1:62587251-62587270 None:intergenic 30.0%
!! GATTTTGATCGTTTAGAGTT+TGG - chr3_1:62587258-62587277 Msa0367070:CDS 30.0%
AGATCTTGATCAGGAAACTT+CGG - chr3_1:62586182-62586201 Msa0367070:CDS 35.0%
!! TGATGGAATGGTGTCATATT+AGG + chr3_1:62586232-62586251 None:intergenic 35.0%
GTGCAGCATAAATACTTTGA+TGG + chr3_1:62586249-62586268 None:intergenic 35.0%
AATTGAGATGGCAAAAAACG+TGG + chr3_1:62586558-62586577 None:intergenic 35.0%
TCCTTTAAGTGCAATTGAGA+TGG + chr3_1:62586570-62586589 None:intergenic 35.0%
ATCAATTCCTGCACAGAAAA+GGG + chr3_1:62586629-62586648 None:intergenic 35.0%
AATCAATTCCTGCACAGAAA+AGG + chr3_1:62586630-62586649 None:intergenic 35.0%
! TTTTCTGTGCAGGAATTGAT+TGG - chr3_1:62586629-62586648 Msa0367070:intron 35.0%
TTTCTGTGCAGGAATTGATT+GGG - chr3_1:62586630-62586649 Msa0367070:intron 35.0%
! ACAAAAAGGCATGTCATCAT+AGG + chr3_1:62586788-62586807 None:intergenic 35.0%
AGCTCTTTACATATGGAGAT+TGG + chr3_1:62587006-62587025 None:intergenic 35.0%
GAATTGTCAAACACTATCTC+TGG - chr3_1:62587206-62587225 Msa0367070:intron 35.0%
AGATCTTGATCAGGAAACTT+CGG - chr3_1:62586182-62586201 Msa0367070:CDS 35.0%
!! TGATGGAATGGTGTCATATT+AGG + chr3_1:62586232-62586251 None:intergenic 35.0%
GTGCAGCATAAATACTTTGA+TGG + chr3_1:62586249-62586268 None:intergenic 35.0%
AATTGAGATGGCAAAAAACG+TGG + chr3_1:62586558-62586577 None:intergenic 35.0%
TCCTTTAAGTGCAATTGAGA+TGG + chr3_1:62586570-62586589 None:intergenic 35.0%
ATCAATTCCTGCACAGAAAA+GGG + chr3_1:62586629-62586648 None:intergenic 35.0%
AATCAATTCCTGCACAGAAA+AGG + chr3_1:62586630-62586649 None:intergenic 35.0%
! TTTTCTGTGCAGGAATTGAT+TGG - chr3_1:62586629-62586648 Msa0367070:intron 35.0%
TTTCTGTGCAGGAATTGATT+GGG - chr3_1:62586630-62586649 Msa0367070:intron 35.0%
! ACAAAAAGGCATGTCATCAT+AGG + chr3_1:62586788-62586807 None:intergenic 35.0%
AGCTCTTTACATATGGAGAT+TGG + chr3_1:62587006-62587025 None:intergenic 35.0%
GAATTGTCAAACACTATCTC+TGG - chr3_1:62587206-62587225 Msa0367070:intron 35.0%
TAGCTACTAGCATTTAAGGG+AGG - chr3_1:62586382-62586401 Msa0367070:CDS 40.0%
AGCTACTAGCATTTAAGGGA+GGG - chr3_1:62586383-62586402 Msa0367070:CDS 40.0%
! TTAAAGGGTAGCGGTAAAAG+AGG - chr3_1:62586491-62586510 Msa0367070:CDS 40.0%
GCCATCTCAATTGCACTTAA+AGG - chr3_1:62586566-62586585 Msa0367070:CDS 40.0%
TCAATTCCTGCACAGAAAAG+GGG + chr3_1:62586628-62586647 None:intergenic 40.0%
! TTTGGGAAGCATGTCTTTGA+GGG + chr3_1:62586934-62586953 None:intergenic 40.0%
CTCAGGAAGCTCTTTACATA+TGG + chr3_1:62587013-62587032 None:intergenic 40.0%
! ATCACTAGACACTTTAGCTC+AGG + chr3_1:62587030-62587049 None:intergenic 40.0%
GATAAGAGAAGCAGATGAGT+TGG - chr3_1:62587047-62587066 Msa0367070:intron 40.0%
TGAAGGTGATGATCACAAAG+TGG - chr3_1:62587086-62587105 Msa0367070:intron 40.0%
! GATCGTTTAGAGTTTGGCTA+CGG - chr3_1:62587264-62587283 Msa0367070:CDS 40.0%
TAGCTACTAGCATTTAAGGG+AGG - chr3_1:62586382-62586401 Msa0367070:CDS 40.0%
AGCTACTAGCATTTAAGGGA+GGG - chr3_1:62586383-62586402 Msa0367070:CDS 40.0%
! TTAAAGGGTAGCGGTAAAAG+AGG - chr3_1:62586491-62586510 Msa0367070:CDS 40.0%
GCCATCTCAATTGCACTTAA+AGG - chr3_1:62586566-62586585 Msa0367070:CDS 40.0%
TCAATTCCTGCACAGAAAAG+GGG + chr3_1:62586628-62586647 None:intergenic 40.0%
! TTTGGGAAGCATGTCTTTGA+GGG + chr3_1:62586934-62586953 None:intergenic 40.0%
CTCAGGAAGCTCTTTACATA+TGG + chr3_1:62587013-62587032 None:intergenic 40.0%
! ATCACTAGACACTTTAGCTC+AGG + chr3_1:62587030-62587049 None:intergenic 40.0%
GATAAGAGAAGCAGATGAGT+TGG - chr3_1:62587047-62587066 Msa0367070:intron 40.0%
TGAAGGTGATGATCACAAAG+TGG - chr3_1:62587086-62587105 Msa0367070:intron 40.0%
! GATCGTTTAGAGTTTGGCTA+CGG - chr3_1:62587264-62587283 Msa0367070:CDS 40.0%
TTCGGAAAGCAGAATCGAAG+CGG - chr3_1:62586200-62586219 Msa0367070:CDS 45.0%
! TATTTCCCCCTTTTCTGTGC+AGG - chr3_1:62586619-62586638 Msa0367070:intron 45.0%
CAATTCCTGCACAGAAAAGG+GGG + chr3_1:62586627-62586646 None:intergenic 45.0%
GCAGGAATTGATTGGGAATG+TGG - chr3_1:62586637-62586656 Msa0367070:intron 45.0%
! CTTTGGGAAGCATGTCTTTG+AGG + chr3_1:62586935-62586954 None:intergenic 45.0%
GAATTCTCTGGACATGACTG+AGG + chr3_1:62587155-62587174 None:intergenic 45.0%
TTCGGAAAGCAGAATCGAAG+CGG - chr3_1:62586200-62586219 Msa0367070:CDS 45.0%
! TATTTCCCCCTTTTCTGTGC+AGG - chr3_1:62586619-62586638 Msa0367070:intron 45.0%
CAATTCCTGCACAGAAAAGG+GGG + chr3_1:62586627-62586646 None:intergenic 45.0%
GCAGGAATTGATTGGGAATG+TGG - chr3_1:62586637-62586656 Msa0367070:intron 45.0%
! CTTTGGGAAGCATGTCTTTG+AGG + chr3_1:62586935-62586954 None:intergenic 45.0%
GAATTCTCTGGACATGACTG+AGG + chr3_1:62587155-62587174 None:intergenic 45.0%
!! GGGAGGGTGCTCAAGTACTT+TGG - chr3_1:62586399-62586418 Msa0367070:CDS 55.0%
CTCTGGACATGACTGAGGCT+TGG + chr3_1:62587150-62587169 None:intergenic 55.0%
!! GGGAGGGTGCTCAAGTACTT+TGG - chr3_1:62586399-62586418 Msa0367070:CDS 55.0%
CTCTGGACATGACTGAGGCT+TGG + chr3_1:62587150-62587169 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 62586159 62587311 62586159 ID=Msa0367070;Name=Msa0367070
chr3_1 mRNA 62586159 62587311 62586159 ID=Msa0367070-mRNA-1;Parent=Msa0367070;Name=Msa0367070-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0|0|0.66|1|1|3|0|220
chr3_1 exon 62587249 62587311 62587249 ID=Msa0367070-mRNA-1:exon:12202;Parent=Msa0367070-mRNA-1
chr3_1 exon 62586696 62586829 62586696 ID=Msa0367070-mRNA-1:exon:12201;Parent=Msa0367070-mRNA-1
chr3_1 exon 62586159 62586624 62586159 ID=Msa0367070-mRNA-1:exon:12200;Parent=Msa0367070-mRNA-1
chr3_1 CDS 62587249 62587311 62587249 ID=Msa0367070-mRNA-1:cds;Parent=Msa0367070-mRNA-1
chr3_1 CDS 62586696 62586829 62586696 ID=Msa0367070-mRNA-1:cds;Parent=Msa0367070-mRNA-1
chr3_1 CDS 62586159 62586624 62586159 ID=Msa0367070-mRNA-1:cds;Parent=Msa0367070-mRNA-1
Gene Sequence

>Msa0367070

TTGAATAAAATTGAAAGAACAATAGATCTTGATCAGGAAACTTCGGAAAGCAGAATCGAAGCGGAATTGATTGGGAATGTGGAGCATTTTATCACTTTGAAAAGACTTCAGGTAGATGATTCAAAAGTAGAAAGTATCTTTTGTCTCAATGAAATCAATGAACAGGAAATGAATTTAGGTTTTGAAGACATTGATTTGTGTCTACCACAGTTGCTTTATATGAGAATTAAAGAATGCAAAGAGTTGAAGCATATTATTGAAGATGATTTGGAGAGTAAAAAACCCTCAAAGACATGCTTCCCAAAGTTAAACATACTTGTTGTAGAAAAGTGCAACAAGTTGAAATATGTGTTTCCAATCTCCATATGTAAAGAGCTTCCTGAGCTAAAGTGTCTAGTGATAAGAGAAGCAGATGAGTTGGATGAAATATTTGCAAATGAAGGTGATGATCACAAAGTGGAGATTCCAAATCTACAATTTGTAGTATTTGAAAATCTTCCAAGCCTCAGTCATGTCCAGAGAATTCATTTTCAAACTGTAAAACACTGTTTCATACAGAATTGTCAAACACTATCTCTGGCTTCATCATCAACAACAGACCTCGAAAATGATTTTGATCGTTTAGAGTTTGGCTACGGTACACACTCTATCCATTATTTCTAA

Protein sequence

>Msa0367070

LNKIERTIDLDQETSESRIEAELIGNVEHFITLKRLQVDDSKVESIFCLNEINEQEMNLGFEDIDLCLPQLLYMRIKECKELKHIIEDDLESKKPSKTCFPKLNILVVEKCNKLKYVFPISICKELPELKCLVIREADELDEIFANEGDDHKVEIPNLQFVVFENLPSLSHVQRIHFQTVKHCFIQNCQTLSLASSSTTDLENDFDRLEFGYGTHSIHYF*