AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0368140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0368140 MtrunA17_Chr3g0110481 92.391 184 14 0 1 184 1 184 2.33e-110 312
Msa0368140 MtrunA17_Chr5g0434691 62.838 148 49 3 1 146 1 144 7.44e-48 154
Msa0368140 MtrunA17_Chr8g0392851 37.705 183 82 8 14 176 28 198 1.31e-15 73.9
Msa0368140 MtrunA17_Chr6g0482691 49.254 67 31 2 14 77 43 109 5.44e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0368140 AT1G14900.1 42.162 185 91 4 11 182 21 202 2.87e-37 128
Msa0368140 AT1G48620.1 50.000 62 31 0 16 77 78 139 7.84e-14 69.3
Msa0368140 AT3G18035.2 46.575 73 39 0 14 86 66 138 8.07e-14 68.9
Msa0368140 AT3G18035.1 46.575 73 39 0 14 86 66 138 8.31e-14 68.9

Find 87 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCGGCCGGGACATCTTTC+TGG 0.115281 3_1:+64268468 None:intergenic
TAAACAATTACACTCTATTT+AGG 0.154977 3_1:+64268273 None:intergenic
AACTCTACTTGTTCTTATTA+AGG 0.203159 3_1:-64269998 Msa0368140:five_prime_UTR
TAGAGTGTAATTGTTTAGTT+TGG 0.223116 3_1:-64268267 Msa0368140:three_prime_UTR
AATGTTGTTGATTTCATCTT+TGG 0.225301 3_1:+64268376 None:intergenic
CCCCTTCTAGGTGGAGCATT+TGG 0.227173 3_1:+64268646 None:intergenic
GGTCTTCCACGACCCCTTCT+AGG 0.272949 3_1:+64268634 None:intergenic
CCGGAGGTAGTGGGAGGCCT+AGG 0.290788 3_1:-64268408 Msa0368140:CDS
GGTGGGCCATTTGGATCCTT+TGG 0.296708 3_1:+64268544 None:intergenic
ACAGTGGTGAATTAGTATTT+TGG 0.301089 3_1:-64268693 Msa0368140:CDS
GAAGGATTAAATGAAGAAAA+TGG 0.328424 3_1:-64268817 Msa0368140:CDS
GAAGGCCTTGGTGGGCCATT+TGG 0.334212 3_1:+64268535 None:intergenic
TGGAGGACGGCCCCTAGGCT+TGG 0.343617 3_1:+64268564 None:intergenic
CCCTTCTAGGTGGAGCATTT+GGG 0.348663 3_1:+64268647 None:intergenic
AGAGTGTAATTGTTTAGTTT+GGG 0.355684 3_1:-64268266 Msa0368140:three_prime_UTR
GGAGGACGGCCCCTAGGCTT+GGG 0.364134 3_1:+64268565 None:intergenic
CTTTGACGGACCATTGCCTT+TGG 0.368500 3_1:-64268440 Msa0368140:CDS
GTTGTTGATTTCATCTTTGG+AGG 0.383514 3_1:+64268379 None:intergenic
ACCCAAATGCTCCACCTAGA+AGG 0.393865 3_1:-64268648 Msa0368140:CDS
ATCGGCCGGGACATCTTTCT+GGG 0.416906 3_1:+64268469 None:intergenic
GCTGTAGGGACCGGAGGTAG+TGG 0.436279 3_1:-64268418 Msa0368140:CDS
ATCTTTCTGGGCCGACCTCT+AGG 0.443975 3_1:+64268481 None:intergenic
TAACGCTTAATGATTGATCA+TGG 0.444438 3_1:-64268344 Msa0368140:exon
GTGACTCATGTGAGCTCTGT+TGG 0.445552 3_1:-64268184 Msa0368140:three_prime_UTR
TTCACTAGTGAAGGCCTTGG+TGG 0.446932 3_1:+64268526 None:intergenic
TTGCCTTTGGCTGTAGGGAC+CGG 0.460950 3_1:-64268427 Msa0368140:CDS
CATTTGGATCCTTTGGAGGA+CGG 0.462369 3_1:+64268551 None:intergenic
GTATGTGGATTCTATGTACT+TGG 0.467423 3_1:+64268774 None:intergenic
AGAAAGATGTCCCGGCCGAT+TGG 0.469889 3_1:-64268466 Msa0368140:CDS
CGGCCGATTGGAGCCTTTGA+CGG 0.471869 3_1:-64268454 Msa0368140:CDS
CGTCAAAGGCTCCAATCGGC+CGG 0.483774 3_1:+64268455 None:intergenic
TGGAGCATTTGGGTCACGCT+TGG 0.494330 3_1:+64268657 None:intergenic
CCTTCACTAGTGAAGACATC+TGG 0.496866 3_1:-64268517 Msa0368140:CDS
GTCGGCCCAGAAAGATGTCC+CGG 0.497280 3_1:-64268474 Msa0368140:CDS
ATCTTTGGAGGTCTACCCCT+AGG 0.498655 3_1:+64268391 None:intergenic
CGGAGGTAGTGGGAGGCCTA+GGG 0.498777 3_1:-64268407 Msa0368140:CDS
GACCATTGCCTTTGGCTGTA+GGG 0.500244 3_1:-64268432 Msa0368140:CDS
GGTTGTGAGTACGTGGCTCC+CGG 0.501306 3_1:+64268586 None:intergenic
CCCAAATGCTCCACCTAGAA+GGG 0.508074 3_1:-64268647 Msa0368140:CDS
GGGCCATTTGGATCCTTTGG+AGG 0.508423 3_1:+64268547 None:intergenic
ACTTGCACCTTGCAAGAATG+AGG 0.510068 3_1:-64268717 Msa0368140:CDS
CACTGTCCCTCATTCTTGCA+AGG 0.512464 3_1:+64268710 None:intergenic
CGTACTCACAACCCAAGCCT+AGG 0.513211 3_1:-64268576 Msa0368140:CDS
CGGTAAGATGGAAGTGAAAG+AGG 0.513286 3_1:+64269893 None:intergenic
GGACCATTGCCTTTGGCTGT+AGG 0.517320 3_1:-64268433 Msa0368140:CDS
GTGAAGACATCTGGTGGGAC+TGG 0.522258 3_1:-64268508 Msa0368140:CDS
TTGAGGCAATCCACCGTATG+TGG 0.529780 3_1:+64268759 None:intergenic
AAATTTACCTCACGGTAAGA+TGG 0.532072 3_1:+64269881 None:intergenic
GTCTTCACTAGTGAAGGCCT+TGG 0.533540 3_1:+64268523 None:intergenic
TCACTAGTGAAGGCCTTGGT+GGG 0.535759 3_1:+64268527 None:intergenic
CTGTAGGGACCGGAGGTAGT+GGG 0.542136 3_1:-64268417 Msa0368140:CDS
TCCTTTGGAGGACGGCCCCT+AGG 0.542137 3_1:+64268559 None:intergenic
GTACTCACAACCCAAGCCTA+GGG 0.543670 3_1:-64268575 Msa0368140:CDS
GCCTAGGGGCCGTCCTCCAA+AGG 0.547952 3_1:-64268560 Msa0368140:CDS
GTCCCTACAGCCAAAGGCAA+TGG 0.557962 3_1:+64268430 None:intergenic
CGTCCTCCAAAGGATCCAAA+TGG 0.558781 3_1:-64268550 Msa0368140:CDS
CCTAGGCCTCCCACTACCTC+CGG 0.558992 3_1:+64268408 None:intergenic
CAAAGGCAATGGTCCGTCAA+AGG 0.560473 3_1:+64268441 None:intergenic
AAAGATGAAATCAACAACAT+TGG 0.561439 3_1:-64268374 Msa0368140:CDS
GGTCCGTCAAAGGCTCCAAT+CGG 0.569515 3_1:+64268451 None:intergenic
GCTCCACCTAGAAGGGGTCG+TGG 0.572176 3_1:-64268640 Msa0368140:CDS
GTCAAAGGCTCCAATCGGCC+GGG 0.572587 3_1:+64268456 None:intergenic
GAGTGTAATTGTTTAGTTTG+GGG 0.576450 3_1:-64268265 Msa0368140:three_prime_UTR
CCAGATGTCTTCACTAGTGA+AGG 0.581390 3_1:+64268517 None:intergenic
TAGGGACCGGAGGTAGTGGG+AGG 0.591716 3_1:-64268414 Msa0368140:CDS
CCTCCGGTCCCTACAGCCAA+AGG 0.597204 3_1:+64268424 None:intergenic
AAAGACTCTTTAGCCTTGGG+TGG 0.601047 3_1:+64268613 None:intergenic
CCTTTGGCTGTAGGGACCGG+AGG 0.607285 3_1:-64268424 Msa0368140:CDS
AGGCTTGGGTTGTGAGTACG+TGG 0.616227 3_1:+64268579 None:intergenic
TCACTAGTGAAGACATCTGG+TGG 0.625386 3_1:-64268514 Msa0368140:CDS
TGGATTGCCTCAAGGACACA+AGG 0.627149 3_1:-64268749 Msa0368140:CDS
TTCACTTCCATCTTACCGTG+AGG 0.629029 3_1:-64269888 Msa0368140:intron
CTTCCACGACCCCTTCTAGG+TGG 0.632258 3_1:+64268637 None:intergenic
GGACTGGAAGACCTAGAGGT+CGG 0.637508 3_1:-64268492 Msa0368140:CDS
GGGTCGTGGAAGACCACCCA+AGG 0.638128 3_1:-64268626 Msa0368140:CDS
CTTGCACCTTGCAAGAATGA+GGG 0.643739 3_1:-64268716 Msa0368140:CDS
ACATACGGTGGATTGCCTCA+AGG 0.646457 3_1:-64268757 Msa0368140:CDS
AGTAGCGCCTTGTGTCCTTG+AGG 0.647299 3_1:+64268742 None:intergenic
CACTAGTGAAGACATCTGGT+GGG 0.653423 3_1:-64268513 Msa0368140:CDS
AAGTACATAGAATCCACATA+CGG 0.657762 3_1:-64268772 Msa0368140:CDS
GGTGGGACTGGAAGACCTAG+AGG 0.665438 3_1:-64268496 Msa0368140:CDS
TACATAGAATCCACATACGG+TGG 0.665446 3_1:-64268769 Msa0368140:CDS
GGAGGTAGTGGGAGGCCTAG+GGG 0.670220 3_1:-64268406 Msa0368140:CDS
CCAAATGCTCCACCTAGAAG+GGG 0.671648 3_1:-64268646 Msa0368140:CDS
AGGATCCAAATGGCCCACCA+AGG 0.671712 3_1:-64268540 Msa0368140:CDS
CTTGCAAGAATGAGGGACAG+TGG 0.678671 3_1:-64268709 Msa0368140:CDS
TACTCACAACCCAAGCCTAG+GGG 0.679795 3_1:-64268574 Msa0368140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATATTTAATTAAT+TGG - chr3_1:64268761-64268780 Msa0368140:CDS 0.0%
!! AATAAAAATATTTAATTAAT+TGG - chr3_1:64268761-64268780 Msa0368140:CDS 0.0%
!! TAAAGATATAAACTTAATTA+AGG + chr3_1:64268624-64268643 None:intergenic 10.0%
!! TTATAAATTCATTAAACAAT+AGG + chr3_1:64268799-64268818 None:intergenic 10.0%
!! TGTTTAATGAATTTATAATA+AGG - chr3_1:64268801-64268820 Msa0368140:CDS 10.0%
!! AAATATTAGTATTACTTTAA+AGG - chr3_1:64269000-64269019 Msa0368140:intron 10.0%
!! TAAAGATATAAACTTAATTA+AGG + chr3_1:64268624-64268643 None:intergenic 10.0%
!! TTATAAATTCATTAAACAAT+AGG + chr3_1:64268799-64268818 None:intergenic 10.0%
!! TGTTTAATGAATTTATAATA+AGG - chr3_1:64268801-64268820 Msa0368140:CDS 10.0%
!! AAATATTAGTATTACTTTAA+AGG - chr3_1:64269000-64269019 Msa0368140:intron 10.0%
!! TAAAATACTAATGAAAAAGA+GGG + chr3_1:64268310-64268329 None:intergenic 15.0%
!! ATAAAATACTAATGAAAAAG+AGG + chr3_1:64268311-64268330 None:intergenic 15.0%
!! TAAACATATGATTAATGAAA+TGG + chr3_1:64268463-64268482 None:intergenic 15.0%
!!! AATTTTAGATTACAAATATG+TGG - chr3_1:64268682-64268701 Msa0368140:CDS 15.0%
!!! CTTTTCAATTTTTAATGAAT+TGG + chr3_1:64269084-64269103 None:intergenic 15.0%
!! TTTGAAAATTGTAAAAATTG+TGG - chr3_1:64269997-64270016 Msa0368140:five_prime_UTR 15.0%
!! TAAAATACTAATGAAAAAGA+GGG + chr3_1:64268310-64268329 None:intergenic 15.0%
!! ATAAAATACTAATGAAAAAG+AGG + chr3_1:64268311-64268330 None:intergenic 15.0%
!! TAAACATATGATTAATGAAA+TGG + chr3_1:64268463-64268482 None:intergenic 15.0%
!!! AATTTTAGATTACAAATATG+TGG - chr3_1:64268682-64268701 Msa0368140:CDS 15.0%
!!! CTTTTCAATTTTTAATGAAT+TGG + chr3_1:64269084-64269103 None:intergenic 15.0%
!! TTTGAAAATTGTAAAAATTG+TGG - chr3_1:64269997-64270016 Msa0368140:five_prime_UTR 15.0%
!!! AAAGAAGTTAGTGAATATTT+TGG + chr3_1:64268553-64268572 None:intergenic 20.0%
!!! TTTTTTTTTTGTGATGTTTG+AGG + chr3_1:64268890-64268909 None:intergenic 20.0%
!!! TGATAATTTTGAACAATTGT+CGG + chr3_1:64269264-64269283 None:intergenic 20.0%
!! TAAACAATTACACTCTATTT+AGG + chr3_1:64269888-64269907 None:intergenic 20.0%
!!! AAAGAAGTTAGTGAATATTT+TGG + chr3_1:64268553-64268572 None:intergenic 20.0%
!!! TTTTTTTTTTGTGATGTTTG+AGG + chr3_1:64268890-64268909 None:intergenic 20.0%
!!! TGATAATTTTGAACAATTGT+CGG + chr3_1:64269264-64269283 None:intergenic 20.0%
!! TAAACAATTACACTCTATTT+AGG + chr3_1:64269888-64269907 None:intergenic 20.0%
! AACTCTACTTGTTCTTATTA+AGG - chr3_1:64268160-64268179 Msa0368140:three_prime_UTR 25.0%
!! AGATATTAGTCTATGCTAAT+CGG + chr3_1:64268353-64268372 None:intergenic 25.0%
!! AATATCTGTTTGAGATTTGT+TGG - chr3_1:64268366-64268385 Msa0368140:CDS 25.0%
! ATGATTAATGAAATGGTCAA+AGG + chr3_1:64268456-64268475 None:intergenic 25.0%
! CATTAAACAATAGGTTTATG+AGG + chr3_1:64268790-64268809 None:intergenic 25.0%
!! TTACTTTAAAGGTAAATGCT+AGG - chr3_1:64269011-64269030 Msa0368140:intron 25.0%
!! TACTTTAAAGGTAAATGCTA+GGG - chr3_1:64269012-64269031 Msa0368140:intron 25.0%
!! TTTAACTGTGATTAGTTGAT+GGG - chr3_1:64269223-64269242 Msa0368140:intron 25.0%
!!! CATTTTTCAGATGATTTTGA+AGG - chr3_1:64269313-64269332 Msa0368140:intron 25.0%
! GAAGGATTAAATGAAGAAAA+TGG - chr3_1:64269341-64269360 Msa0368140:intron 25.0%
!!! AATGTTGTTGATTTCATCTT+TGG + chr3_1:64269785-64269804 None:intergenic 25.0%
! AAAGATGAAATCAACAACAT+TGG - chr3_1:64269784-64269803 Msa0368140:intron 25.0%
! TAGAGTGTAATTGTTTAGTT+TGG - chr3_1:64269891-64269910 Msa0368140:CDS 25.0%
!! AGAGTGTAATTGTTTAGTTT+GGG - chr3_1:64269892-64269911 Msa0368140:CDS 25.0%
! AACTCTACTTGTTCTTATTA+AGG - chr3_1:64268160-64268179 Msa0368140:three_prime_UTR 25.0%
!! AGATATTAGTCTATGCTAAT+CGG + chr3_1:64268353-64268372 None:intergenic 25.0%
!! AATATCTGTTTGAGATTTGT+TGG - chr3_1:64268366-64268385 Msa0368140:CDS 25.0%
! ATGATTAATGAAATGGTCAA+AGG + chr3_1:64268456-64268475 None:intergenic 25.0%
! CATTAAACAATAGGTTTATG+AGG + chr3_1:64268790-64268809 None:intergenic 25.0%
!! TTACTTTAAAGGTAAATGCT+AGG - chr3_1:64269011-64269030 Msa0368140:intron 25.0%
!! TACTTTAAAGGTAAATGCTA+GGG - chr3_1:64269012-64269031 Msa0368140:intron 25.0%
!! TTTAACTGTGATTAGTTGAT+GGG - chr3_1:64269223-64269242 Msa0368140:intron 25.0%
!!! CATTTTTCAGATGATTTTGA+AGG - chr3_1:64269313-64269332 Msa0368140:intron 25.0%
! GAAGGATTAAATGAAGAAAA+TGG - chr3_1:64269341-64269360 Msa0368140:intron 25.0%
!!! AATGTTGTTGATTTCATCTT+TGG + chr3_1:64269785-64269804 None:intergenic 25.0%
! AAAGATGAAATCAACAACAT+TGG - chr3_1:64269784-64269803 Msa0368140:intron 25.0%
! TAGAGTGTAATTGTTTAGTT+TGG - chr3_1:64269891-64269910 Msa0368140:CDS 25.0%
!! AGAGTGTAATTGTTTAGTTT+GGG - chr3_1:64269892-64269911 Msa0368140:CDS 25.0%
!!! AACTCTTCTATCGACTTTTA+TGG - chr3_1:64268219-64268238 Msa0368140:three_prime_UTR 30.0%
AAATATGTGGAGTTAGAGAA+AGG - chr3_1:64268695-64268714 Msa0368140:CDS 30.0%
GATAATGCAGAATATATGCA+AGG - chr3_1:64268851-64268870 Msa0368140:intron 30.0%
!! AGAATGTCTAAAGAGTGTTA+TGG - chr3_1:64269136-64269155 Msa0368140:intron 30.0%
!! GAATGTCTAAAGAGTGTTAT+GGG - chr3_1:64269137-64269156 Msa0368140:intron 30.0%
! GTTTAACTGTGATTAGTTGA+TGG - chr3_1:64269222-64269241 Msa0368140:intron 30.0%
!! ATGATTTTGAAGGCAATAGA+AGG - chr3_1:64269323-64269342 Msa0368140:intron 30.0%
AAGTACATAGAATCCACATA+CGG - chr3_1:64269386-64269405 Msa0368140:intron 30.0%
! ACAGTGGTGAATTAGTATTT+TGG - chr3_1:64269465-64269484 Msa0368140:intron 30.0%
TAACGCTTAATGATTGATCA+TGG - chr3_1:64269814-64269833 Msa0368140:intron 30.0%
! TTTGCATTTTATGTCTTGCA+TGG - chr3_1:64269837-64269856 Msa0368140:intron 30.0%
!! GAGTGTAATTGTTTAGTTTG+GGG - chr3_1:64269893-64269912 Msa0368140:CDS 30.0%
!!! AACTCTTCTATCGACTTTTA+TGG - chr3_1:64268219-64268238 Msa0368140:three_prime_UTR 30.0%
AAATATGTGGAGTTAGAGAA+AGG - chr3_1:64268695-64268714 Msa0368140:CDS 30.0%
GATAATGCAGAATATATGCA+AGG - chr3_1:64268851-64268870 Msa0368140:intron 30.0%
!! AGAATGTCTAAAGAGTGTTA+TGG - chr3_1:64269136-64269155 Msa0368140:intron 30.0%
!! GAATGTCTAAAGAGTGTTAT+GGG - chr3_1:64269137-64269156 Msa0368140:intron 30.0%
! GTTTAACTGTGATTAGTTGA+TGG - chr3_1:64269222-64269241 Msa0368140:intron 30.0%
!! ATGATTTTGAAGGCAATAGA+AGG - chr3_1:64269323-64269342 Msa0368140:intron 30.0%
AAGTACATAGAATCCACATA+CGG - chr3_1:64269386-64269405 Msa0368140:intron 30.0%
! ACAGTGGTGAATTAGTATTT+TGG - chr3_1:64269465-64269484 Msa0368140:intron 30.0%
TAACGCTTAATGATTGATCA+TGG - chr3_1:64269814-64269833 Msa0368140:intron 30.0%
! TTTGCATTTTATGTCTTGCA+TGG - chr3_1:64269837-64269856 Msa0368140:intron 30.0%
!! GAGTGTAATTGTTTAGTTTG+GGG - chr3_1:64269893-64269912 Msa0368140:CDS 30.0%
!!! GTTTTGAAGCTTTACAACCT+CGG + chr3_1:64268202-64268221 None:intergenic 35.0%
AAATTTACCTCACGGTAAGA+TGG + chr3_1:64268280-64268299 None:intergenic 35.0%
GTTAGCTGAAATTTACCTCA+CGG + chr3_1:64268288-64268307 None:intergenic 35.0%
! TATTAGTCTATGCTAATCGG+CGG + chr3_1:64268350-64268369 None:intergenic 35.0%
GTATGTGGATTCTATGTACT+TGG + chr3_1:64269387-64269406 None:intergenic 35.0%
! GAAAAAGACTCTTTAGCCTT+GGG + chr3_1:64269551-64269570 None:intergenic 35.0%
!! GTTGTTGATTTCATCTTTGG+AGG + chr3_1:64269782-64269801 None:intergenic 35.0%
!!! GTTTTGAAGCTTTACAACCT+CGG + chr3_1:64268202-64268221 None:intergenic 35.0%
AAATTTACCTCACGGTAAGA+TGG + chr3_1:64268280-64268299 None:intergenic 35.0%
GTTAGCTGAAATTTACCTCA+CGG + chr3_1:64268288-64268307 None:intergenic 35.0%
! TATTAGTCTATGCTAATCGG+CGG + chr3_1:64268350-64268369 None:intergenic 35.0%
GTATGTGGATTCTATGTACT+TGG + chr3_1:64269387-64269406 None:intergenic 35.0%
! GAAAAAGACTCTTTAGCCTT+GGG + chr3_1:64269551-64269570 None:intergenic 35.0%
!! GTTGTTGATTTCATCTTTGG+AGG + chr3_1:64269782-64269801 None:intergenic 35.0%
!!! GTTGTTTTTCTGAAGATCCG+AGG - chr3_1:64268182-64268201 Msa0368140:three_prime_UTR 40.0%
!!! CTATCGACTTTTATGGCTAC+TGG - chr3_1:64268226-64268245 Msa0368140:three_prime_UTR 40.0%
! TGAGATTACCTCGGTTTGTA+AGG - chr3_1:64268829-64268848 Msa0368140:CDS 40.0%
TGCATTATCCTTACAAACCG+AGG + chr3_1:64268840-64268859 None:intergenic 40.0%
TGCAGAATATATGCAAGGTC+TGG - chr3_1:64268856-64268875 Msa0368140:intron 40.0%
GCAGAATATATGCAAGGTCT+GGG - chr3_1:64268857-64268876 Msa0368140:intron 40.0%
CAGAATATATGCAAGGTCTG+GGG - chr3_1:64268858-64268877 Msa0368140:intron 40.0%
! AAATGCTAGGGCACTAGTTA+AGG - chr3_1:64269024-64269043 Msa0368140:intron 40.0%
!! TAAAGAGTGTTATGGGACAC+TGG - chr3_1:64269144-64269163 Msa0368140:intron 40.0%
TACATAGAATCCACATACGG+TGG - chr3_1:64269389-64269408 Msa0368140:intron 40.0%
CGAAAAAGACTCTTTAGCCT+TGG + chr3_1:64269552-64269571 None:intergenic 40.0%
!!! GTTGTTTTTCTGAAGATCCG+AGG - chr3_1:64268182-64268201 Msa0368140:three_prime_UTR 40.0%
!!! CTATCGACTTTTATGGCTAC+TGG - chr3_1:64268226-64268245 Msa0368140:three_prime_UTR 40.0%
! TGAGATTACCTCGGTTTGTA+AGG - chr3_1:64268829-64268848 Msa0368140:CDS 40.0%
TGCATTATCCTTACAAACCG+AGG + chr3_1:64268840-64268859 None:intergenic 40.0%
TGCAGAATATATGCAAGGTC+TGG - chr3_1:64268856-64268875 Msa0368140:intron 40.0%
GCAGAATATATGCAAGGTCT+GGG - chr3_1:64268857-64268876 Msa0368140:intron 40.0%
CAGAATATATGCAAGGTCTG+GGG - chr3_1:64268858-64268877 Msa0368140:intron 40.0%
! AAATGCTAGGGCACTAGTTA+AGG - chr3_1:64269024-64269043 Msa0368140:intron 40.0%
!! TAAAGAGTGTTATGGGACAC+TGG - chr3_1:64269144-64269163 Msa0368140:intron 40.0%
TACATAGAATCCACATACGG+TGG - chr3_1:64269389-64269408 Msa0368140:intron 40.0%
CGAAAAAGACTCTTTAGCCT+TGG + chr3_1:64269552-64269571 None:intergenic 40.0%
! GACTTTTATGGCTACTGGAG+TGG - chr3_1:64268231-64268250 Msa0368140:three_prime_UTR 45.0%
CGGTAAGATGGAAGTGAAAG+AGG + chr3_1:64268268-64268287 None:intergenic 45.0%
TTCACTTCCATCTTACCGTG+AGG - chr3_1:64268270-64268289 Msa0368140:three_prime_UTR 45.0%
AAGGTCCTGTGAGATTACCT+CGG - chr3_1:64268820-64268839 Msa0368140:CDS 45.0%
ACAAACCGAGGTAATCTCAC+AGG + chr3_1:64268828-64268847 None:intergenic 45.0%
ACTTGCACCTTGCAAGAATG+AGG - chr3_1:64269441-64269460 Msa0368140:intron 45.0%
CTTGCACCTTGCAAGAATGA+GGG - chr3_1:64269442-64269461 Msa0368140:intron 45.0%
!! AAAGACTCTTTAGCCTTGGG+TGG + chr3_1:64269548-64269567 None:intergenic 45.0%
!! GGCTAAAGAGTCTTTTTCGC+CGG - chr3_1:64269553-64269572 Msa0368140:intron 45.0%
!! GCTAAAGAGTCTTTTTCGCC+GGG - chr3_1:64269554-64269573 Msa0368140:intron 45.0%
! CATTTGGATCCTTTGGAGGA+CGG + chr3_1:64269610-64269629 None:intergenic 45.0%
CCAGATGTCTTCACTAGTGA+AGG + chr3_1:64269644-64269663 None:intergenic 45.0%
CCTTCACTAGTGAAGACATC+TGG - chr3_1:64269641-64269660 Msa0368140:intron 45.0%
TCACTAGTGAAGACATCTGG+TGG - chr3_1:64269644-64269663 Msa0368140:intron 45.0%
CACTAGTGAAGACATCTGGT+GGG - chr3_1:64269645-64269664 Msa0368140:intron 45.0%
! GACTTTTATGGCTACTGGAG+TGG - chr3_1:64268231-64268250 Msa0368140:three_prime_UTR 45.0%
CGGTAAGATGGAAGTGAAAG+AGG + chr3_1:64268268-64268287 None:intergenic 45.0%
TTCACTTCCATCTTACCGTG+AGG - chr3_1:64268270-64268289 Msa0368140:three_prime_UTR 45.0%
AAGGTCCTGTGAGATTACCT+CGG - chr3_1:64268820-64268839 Msa0368140:CDS 45.0%
ACAAACCGAGGTAATCTCAC+AGG + chr3_1:64268828-64268847 None:intergenic 45.0%
ACTTGCACCTTGCAAGAATG+AGG - chr3_1:64269441-64269460 Msa0368140:intron 45.0%
CTTGCACCTTGCAAGAATGA+GGG - chr3_1:64269442-64269461 Msa0368140:intron 45.0%
!! AAAGACTCTTTAGCCTTGGG+TGG + chr3_1:64269548-64269567 None:intergenic 45.0%
!! GGCTAAAGAGTCTTTTTCGC+CGG - chr3_1:64269553-64269572 Msa0368140:intron 45.0%
!! GCTAAAGAGTCTTTTTCGCC+GGG - chr3_1:64269554-64269573 Msa0368140:intron 45.0%
! CATTTGGATCCTTTGGAGGA+CGG + chr3_1:64269610-64269629 None:intergenic 45.0%
CCAGATGTCTTCACTAGTGA+AGG + chr3_1:64269644-64269663 None:intergenic 45.0%
CCTTCACTAGTGAAGACATC+TGG - chr3_1:64269641-64269660 Msa0368140:intron 45.0%
TCACTAGTGAAGACATCTGG+TGG - chr3_1:64269644-64269663 Msa0368140:intron 45.0%
CACTAGTGAAGACATCTGGT+GGG - chr3_1:64269645-64269664 Msa0368140:intron 45.0%
!!! TAATTAAATATTTTTATTGT+TGG + chr3_1:64268759-64268778 None:intergenic 5.0%
!! AATATTATTAATTAAAGTAA+GGG + chr3_1:64269179-64269198 None:intergenic 5.0%
!! AAATATTATTAATTAAAGTA+AGG + chr3_1:64269180-64269199 None:intergenic 5.0%
!!! TAATTAAATATTTTTATTGT+TGG + chr3_1:64268759-64268778 None:intergenic 5.0%
!! AATATTATTAATTAAAGTAA+GGG + chr3_1:64269179-64269198 None:intergenic 5.0%
!! AAATATTATTAATTAAAGTA+AGG + chr3_1:64269180-64269199 None:intergenic 5.0%
TTGAGGCAATCCACCGTATG+TGG + chr3_1:64269402-64269421 None:intergenic 50.0%
!! ACATACGGTGGATTGCCTCA+AGG - chr3_1:64269401-64269420 Msa0368140:intron 50.0%
TGGATTGCCTCAAGGACACA+AGG - chr3_1:64269409-64269428 Msa0368140:intron 50.0%
CACTGTCCCTCATTCTTGCA+AGG + chr3_1:64269451-64269470 None:intergenic 50.0%
CTTGCAAGAATGAGGGACAG+TGG - chr3_1:64269449-64269468 Msa0368140:intron 50.0%
ACCCAAATGCTCCACCTAGA+AGG - chr3_1:64269510-64269529 Msa0368140:intron 50.0%
! CCCTTCTAGGTGGAGCATTT+GGG + chr3_1:64269514-64269533 None:intergenic 50.0%
CCCAAATGCTCCACCTAGAA+GGG - chr3_1:64269511-64269530 Msa0368140:intron 50.0%
CCAAATGCTCCACCTAGAAG+GGG - chr3_1:64269512-64269531 Msa0368140:intron 50.0%
GTACTCACAACCCAAGCCTA+GGG - chr3_1:64269583-64269602 Msa0368140:intron 50.0%
TACTCACAACCCAAGCCTAG+GGG - chr3_1:64269584-64269603 Msa0368140:intron 50.0%
CGTCCTCCAAAGGATCCAAA+TGG - chr3_1:64269608-64269627 Msa0368140:intron 50.0%
TCACTAGTGAAGGCCTTGGT+GGG + chr3_1:64269634-64269653 None:intergenic 50.0%
TTCACTAGTGAAGGCCTTGG+TGG + chr3_1:64269635-64269654 None:intergenic 50.0%
GTCTTCACTAGTGAAGGCCT+TGG + chr3_1:64269638-64269657 None:intergenic 50.0%
CAAAGGCAATGGTCCGTCAA+AGG + chr3_1:64269720-64269739 None:intergenic 50.0%
! CTTTGACGGACCATTGCCTT+TGG - chr3_1:64269718-64269737 Msa0368140:intron 50.0%
GACCATTGCCTTTGGCTGTA+GGG - chr3_1:64269726-64269745 Msa0368140:intron 50.0%
! ATCTTTGGAGGTCTACCCCT+AGG + chr3_1:64269770-64269789 None:intergenic 50.0%
GTGACTCATGTGAGCTCTGT+TGG - chr3_1:64269974-64269993 Msa0368140:five_prime_UTR 50.0%
TTGAGGCAATCCACCGTATG+TGG + chr3_1:64269402-64269421 None:intergenic 50.0%
!! ACATACGGTGGATTGCCTCA+AGG - chr3_1:64269401-64269420 Msa0368140:intron 50.0%
TGGATTGCCTCAAGGACACA+AGG - chr3_1:64269409-64269428 Msa0368140:intron 50.0%
CACTGTCCCTCATTCTTGCA+AGG + chr3_1:64269451-64269470 None:intergenic 50.0%
CTTGCAAGAATGAGGGACAG+TGG - chr3_1:64269449-64269468 Msa0368140:intron 50.0%
ACCCAAATGCTCCACCTAGA+AGG - chr3_1:64269510-64269529 Msa0368140:intron 50.0%
! CCCTTCTAGGTGGAGCATTT+GGG + chr3_1:64269514-64269533 None:intergenic 50.0%
CCCAAATGCTCCACCTAGAA+GGG - chr3_1:64269511-64269530 Msa0368140:intron 50.0%
CCAAATGCTCCACCTAGAAG+GGG - chr3_1:64269512-64269531 Msa0368140:intron 50.0%
GTACTCACAACCCAAGCCTA+GGG - chr3_1:64269583-64269602 Msa0368140:intron 50.0%
TACTCACAACCCAAGCCTAG+GGG - chr3_1:64269584-64269603 Msa0368140:intron 50.0%
CGTCCTCCAAAGGATCCAAA+TGG - chr3_1:64269608-64269627 Msa0368140:intron 50.0%
TCACTAGTGAAGGCCTTGGT+GGG + chr3_1:64269634-64269653 None:intergenic 50.0%
TTCACTAGTGAAGGCCTTGG+TGG + chr3_1:64269635-64269654 None:intergenic 50.0%
GTCTTCACTAGTGAAGGCCT+TGG + chr3_1:64269638-64269657 None:intergenic 50.0%
CAAAGGCAATGGTCCGTCAA+AGG + chr3_1:64269720-64269739 None:intergenic 50.0%
! CTTTGACGGACCATTGCCTT+TGG - chr3_1:64269718-64269737 Msa0368140:intron 50.0%
GACCATTGCCTTTGGCTGTA+GGG - chr3_1:64269726-64269745 Msa0368140:intron 50.0%
! ATCTTTGGAGGTCTACCCCT+AGG + chr3_1:64269770-64269789 None:intergenic 50.0%
GTGACTCATGTGAGCTCTGT+TGG - chr3_1:64269974-64269993 Msa0368140:five_prime_UTR 50.0%
AGTAGCGCCTTGTGTCCTTG+AGG + chr3_1:64269419-64269438 None:intergenic 55.0%
! TGGAGCATTTGGGTCACGCT+TGG + chr3_1:64269504-64269523 None:intergenic 55.0%
! CCCCTTCTAGGTGGAGCATT+TGG + chr3_1:64269515-64269534 None:intergenic 55.0%
! AGGCTTGGGTTGTGAGTACG+TGG + chr3_1:64269582-64269601 None:intergenic 55.0%
CGTACTCACAACCCAAGCCT+AGG - chr3_1:64269582-64269601 Msa0368140:intron 55.0%
GGGCCATTTGGATCCTTTGG+AGG + chr3_1:64269614-64269633 None:intergenic 55.0%
GGTGGGCCATTTGGATCCTT+TGG + chr3_1:64269617-64269636 None:intergenic 55.0%
AGGATCCAAATGGCCCACCA+AGG - chr3_1:64269618-64269637 Msa0368140:intron 55.0%
GTGAAGACATCTGGTGGGAC+TGG - chr3_1:64269650-64269669 Msa0368140:intron 55.0%
GGACTGGAAGACCTAGAGGT+CGG - chr3_1:64269666-64269685 Msa0368140:intron 55.0%
ATCTTTCTGGGCCGACCTCT+AGG + chr3_1:64269680-64269699 None:intergenic 55.0%
ATCGGCCGGGACATCTTTCT+GGG + chr3_1:64269692-64269711 None:intergenic 55.0%
AATCGGCCGGGACATCTTTC+TGG + chr3_1:64269693-64269712 None:intergenic 55.0%
AGAAAGATGTCCCGGCCGAT+TGG - chr3_1:64269692-64269711 Msa0368140:intron 55.0%
GGTCCGTCAAAGGCTCCAAT+CGG + chr3_1:64269710-64269729 None:intergenic 55.0%
GGACCATTGCCTTTGGCTGT+AGG - chr3_1:64269725-64269744 Msa0368140:intron 55.0%
GTCCCTACAGCCAAAGGCAA+TGG + chr3_1:64269731-64269750 None:intergenic 55.0%
TTGCCTTTGGCTGTAGGGAC+CGG - chr3_1:64269731-64269750 Msa0368140:intron 55.0%
AGTAGCGCCTTGTGTCCTTG+AGG + chr3_1:64269419-64269438 None:intergenic 55.0%
! TGGAGCATTTGGGTCACGCT+TGG + chr3_1:64269504-64269523 None:intergenic 55.0%
! CCCCTTCTAGGTGGAGCATT+TGG + chr3_1:64269515-64269534 None:intergenic 55.0%
! AGGCTTGGGTTGTGAGTACG+TGG + chr3_1:64269582-64269601 None:intergenic 55.0%
CGTACTCACAACCCAAGCCT+AGG - chr3_1:64269582-64269601 Msa0368140:intron 55.0%
GGGCCATTTGGATCCTTTGG+AGG + chr3_1:64269614-64269633 None:intergenic 55.0%
GGTGGGCCATTTGGATCCTT+TGG + chr3_1:64269617-64269636 None:intergenic 55.0%
AGGATCCAAATGGCCCACCA+AGG - chr3_1:64269618-64269637 Msa0368140:intron 55.0%
GTGAAGACATCTGGTGGGAC+TGG - chr3_1:64269650-64269669 Msa0368140:intron 55.0%
GGACTGGAAGACCTAGAGGT+CGG - chr3_1:64269666-64269685 Msa0368140:intron 55.0%
ATCTTTCTGGGCCGACCTCT+AGG + chr3_1:64269680-64269699 None:intergenic 55.0%
ATCGGCCGGGACATCTTTCT+GGG + chr3_1:64269692-64269711 None:intergenic 55.0%
AATCGGCCGGGACATCTTTC+TGG + chr3_1:64269693-64269712 None:intergenic 55.0%
AGAAAGATGTCCCGGCCGAT+TGG - chr3_1:64269692-64269711 Msa0368140:intron 55.0%
GGTCCGTCAAAGGCTCCAAT+CGG + chr3_1:64269710-64269729 None:intergenic 55.0%
GGACCATTGCCTTTGGCTGT+AGG - chr3_1:64269725-64269744 Msa0368140:intron 55.0%
GTCCCTACAGCCAAAGGCAA+TGG + chr3_1:64269731-64269750 None:intergenic 55.0%
TTGCCTTTGGCTGTAGGGAC+CGG - chr3_1:64269731-64269750 Msa0368140:intron 55.0%
CTTCCACGACCCCTTCTAGG+TGG + chr3_1:64269524-64269543 None:intergenic 60.0%
GGTCTTCCACGACCCCTTCT+AGG + chr3_1:64269527-64269546 None:intergenic 60.0%
GGTTGTGAGTACGTGGCTCC+CGG + chr3_1:64269575-64269594 None:intergenic 60.0%
! GAAGGCCTTGGTGGGCCATT+TGG + chr3_1:64269626-64269645 None:intergenic 60.0%
GGTGGGACTGGAAGACCTAG+AGG - chr3_1:64269662-64269681 Msa0368140:intron 60.0%
GTCGGCCCAGAAAGATGTCC+CGG - chr3_1:64269684-64269703 Msa0368140:intron 60.0%
GTCAAAGGCTCCAATCGGCC+GGG + chr3_1:64269705-64269724 None:intergenic 60.0%
CGTCAAAGGCTCCAATCGGC+CGG + chr3_1:64269706-64269725 None:intergenic 60.0%
CGGCCGATTGGAGCCTTTGA+CGG - chr3_1:64269704-64269723 Msa0368140:intron 60.0%
CTGTAGGGACCGGAGGTAGT+GGG - chr3_1:64269741-64269760 Msa0368140:intron 60.0%
CTTCCACGACCCCTTCTAGG+TGG + chr3_1:64269524-64269543 None:intergenic 60.0%
GGTCTTCCACGACCCCTTCT+AGG + chr3_1:64269527-64269546 None:intergenic 60.0%
GGTTGTGAGTACGTGGCTCC+CGG + chr3_1:64269575-64269594 None:intergenic 60.0%
! GAAGGCCTTGGTGGGCCATT+TGG + chr3_1:64269626-64269645 None:intergenic 60.0%
GGTGGGACTGGAAGACCTAG+AGG - chr3_1:64269662-64269681 Msa0368140:intron 60.0%
GTCGGCCCAGAAAGATGTCC+CGG - chr3_1:64269684-64269703 Msa0368140:intron 60.0%
GTCAAAGGCTCCAATCGGCC+GGG + chr3_1:64269705-64269724 None:intergenic 60.0%
CGTCAAAGGCTCCAATCGGC+CGG + chr3_1:64269706-64269725 None:intergenic 60.0%
CGGCCGATTGGAGCCTTTGA+CGG - chr3_1:64269704-64269723 Msa0368140:intron 60.0%
CTGTAGGGACCGGAGGTAGT+GGG - chr3_1:64269741-64269760 Msa0368140:intron 60.0%
GCTCCACCTAGAAGGGGTCG+TGG - chr3_1:64269518-64269537 Msa0368140:intron 65.0%
GGGTCGTGGAAGACCACCCA+AGG - chr3_1:64269532-64269551 Msa0368140:intron 65.0%
! TCCTTTGGAGGACGGCCCCT+AGG + chr3_1:64269602-64269621 None:intergenic 65.0%
CCTCCGGTCCCTACAGCCAA+AGG + chr3_1:64269737-64269756 None:intergenic 65.0%
CCTTTGGCTGTAGGGACCGG+AGG - chr3_1:64269734-64269753 Msa0368140:intron 65.0%
GCTGTAGGGACCGGAGGTAG+TGG - chr3_1:64269740-64269759 Msa0368140:intron 65.0%
!! TAGGGACCGGAGGTAGTGGG+AGG - chr3_1:64269744-64269763 Msa0368140:intron 65.0%
CCTAGGCCTCCCACTACCTC+CGG + chr3_1:64269753-64269772 None:intergenic 65.0%
!! CGGAGGTAGTGGGAGGCCTA+GGG - chr3_1:64269751-64269770 Msa0368140:intron 65.0%
!! GGAGGTAGTGGGAGGCCTAG+GGG - chr3_1:64269752-64269771 Msa0368140:intron 65.0%
GCTCCACCTAGAAGGGGTCG+TGG - chr3_1:64269518-64269537 Msa0368140:intron 65.0%
GGGTCGTGGAAGACCACCCA+AGG - chr3_1:64269532-64269551 Msa0368140:intron 65.0%
! TCCTTTGGAGGACGGCCCCT+AGG + chr3_1:64269602-64269621 None:intergenic 65.0%
CCTCCGGTCCCTACAGCCAA+AGG + chr3_1:64269737-64269756 None:intergenic 65.0%
CCTTTGGCTGTAGGGACCGG+AGG - chr3_1:64269734-64269753 Msa0368140:intron 65.0%
GCTGTAGGGACCGGAGGTAG+TGG - chr3_1:64269740-64269759 Msa0368140:intron 65.0%
!! TAGGGACCGGAGGTAGTGGG+AGG - chr3_1:64269744-64269763 Msa0368140:intron 65.0%
CCTAGGCCTCCCACTACCTC+CGG + chr3_1:64269753-64269772 None:intergenic 65.0%
!! CGGAGGTAGTGGGAGGCCTA+GGG - chr3_1:64269751-64269770 Msa0368140:intron 65.0%
!! GGAGGTAGTGGGAGGCCTAG+GGG - chr3_1:64269752-64269771 Msa0368140:intron 65.0%
GGAGGACGGCCCCTAGGCTT+GGG + chr3_1:64269596-64269615 None:intergenic 70.0%
TGGAGGACGGCCCCTAGGCT+TGG + chr3_1:64269597-64269616 None:intergenic 70.0%
GCCTAGGGGCCGTCCTCCAA+AGG - chr3_1:64269598-64269617 Msa0368140:intron 70.0%
!! CCGGAGGTAGTGGGAGGCCT+AGG - chr3_1:64269750-64269769 Msa0368140:intron 70.0%
GGAGGACGGCCCCTAGGCTT+GGG + chr3_1:64269596-64269615 None:intergenic 70.0%
TGGAGGACGGCCCCTAGGCT+TGG + chr3_1:64269597-64269616 None:intergenic 70.0%
GCCTAGGGGCCGTCCTCCAA+AGG - chr3_1:64269598-64269617 Msa0368140:intron 70.0%
!! CCGGAGGTAGTGGGAGGCCT+AGG - chr3_1:64269750-64269769 Msa0368140:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 64268133 64270047 64268133 ID=Msa0368140;Name=Msa0368140
chr3_1 mRNA 64268133 64270047 64268133 ID=Msa0368140-mRNA-1;Parent=Msa0368140;Name=Msa0368140-mRNA-1;_AED=0.02;_eAED=0.02;_QI=105|1|1|1|1|1|2|224|184
chr3_1 exon 64268133 64268857 64268133 ID=Msa0368140-mRNA-1:exon:12830;Parent=Msa0368140-mRNA-1
chr3_1 exon 64269889 64270047 64269889 ID=Msa0368140-mRNA-1:exon:12829;Parent=Msa0368140-mRNA-1
chr3_1 five_prime_UTR 64269943 64270047 64269943 ID=Msa0368140-mRNA-1:five_prime_utr;Parent=Msa0368140-mRNA-1
chr3_1 CDS 64269889 64269942 64269889 ID=Msa0368140-mRNA-1:cds;Parent=Msa0368140-mRNA-1
chr3_1 CDS 64268357 64268857 64268357 ID=Msa0368140-mRNA-1:cds;Parent=Msa0368140-mRNA-1
chr3_1 three_prime_UTR 64268133 64268356 64268133 ID=Msa0368140-mRNA-1:three_prime_utr;Parent=Msa0368140-mRNA-1
Gene Sequence

>Msa0368140

ATGGCTACTGGAGTGGTTATTAATAAACCTCTTTCACTTCCATCTTACCGTGAGATGATTTTGAAGGCAATAGAAGGATTAAATGAAGAAAATGGTTCAAACAAATCATCAATTTCCAAGTACATAGAATCCACATACGGTGGATTGCCTCAAGGACACAAGGCGCTACTCAACTTGCACCTTGCAAGAATGAGGGACAGTGGTGAATTAGTATTTTGGAAAAACAACTACACCAAGCGTGACCCAAATGCTCCACCTAGAAGGGGTCGTGGAAGACCACCCAAGGCTAAAGAGTCTTTTTCGCCGGGAGCCACGTACTCACAACCCAAGCCTAGGGGCCGTCCTCCAAAGGATCCAAATGGCCCACCAAGGCCTTCACTAGTGAAGACATCTGGTGGGACTGGAAGACCTAGAGGTCGGCCCAGAAAGATGTCCCGGCCGATTGGAGCCTTTGACGGACCATTGCCTTTGGCTGTAGGGACCGGAGGTAGTGGGAGGCCTAGGGGTAGACCTCCAAAGATGAAATCAACAACATTGGCTGAAATTAACGCTTAA
Protein sequence

>Msa0368140

MATGVVINKPLSLPSYREMILKAIEGLNEENGSNKSSISKYIESTYGGLPQGHKALLNLHLARMRDSGELVFWKNNYTKRDPNAPPRRGRGRPPKAKESFSPGATYSQPKPRGRPPKDPNGPPRPSLVKTSGGTGRPRGRPRKMSRPIGAFDGPLPLAVGTGGSGRPRGRPPKMKSTTLAEINA*