Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0379740 | XP_024635134.1 | 94.080 | 1081 | 37 | 4 | 1 | 1054 | 1 | 1081 | 0.0 | 1993 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0379740 | sp|A8MUU9|YV023_HUMAN | 28.931 | 477 | 250 | 23 | 474 | 908 | 6 | 435 | 1.27e-17 | 90.9 |
| Msa0379740 | sp|A8MUU9|YV023_HUMAN | 28.000 | 425 | 213 | 20 | 397 | 808 | 145 | 489 | 3.18e-12 | 73.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0379740 | A0A396J4N0 | 94.080 | 1081 | 37 | 4 | 1 | 1054 | 1 | 1081 | 0.0 | 1993 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0379740 | Msa0379760 | 0.987554 | 1.114676e-170 | -8.615850e-47 |
| Msa0379740 | Msa0510490 | 0.817139 | 3.849689e-52 | -8.615850e-47 |
| Msa0379740 | Msa1449420 | 0.987045 | 7.292393e-169 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0379740 | MtrunA17_Chr3g0132851 | 94.080 | 1081 | 37 | 4 | 1 | 1054 | 1 | 1081 | 0.0 | 1993 |
| Msa0379740 | MtrunA17_Chr8g0346141 | 74.344 | 343 | 73 | 6 | 1 | 338 | 1 | 333 | 2.80e-158 | 472 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0379740 | AT1G09710.1 | 43.262 | 282 | 144 | 7 | 7 | 286 | 12 | 279 | 2.18e-59 | 215 |
| Msa0379740 | AT1G09710.4 | 43.262 | 282 | 144 | 7 | 7 | 286 | 12 | 279 | 2.18e-59 | 215 |
| Msa0379740 | AT1G09710.2 | 43.262 | 282 | 144 | 7 | 7 | 286 | 12 | 279 | 9.90e-59 | 214 |
| Msa0379740 | AT1G58220.1 | 41.158 | 311 | 158 | 10 | 5 | 310 | 8 | 298 | 1.23e-57 | 214 |
| Msa0379740 | AT1G58220.2 | 41.290 | 310 | 157 | 10 | 5 | 309 | 8 | 297 | 1.36e-57 | 213 |
| Msa0379740 | AT1G09710.5 | 43.511 | 262 | 132 | 7 | 27 | 286 | 3 | 250 | 1.02e-53 | 198 |
| Msa0379740 | AT1G09710.3 | 43.511 | 262 | 132 | 7 | 27 | 286 | 3 | 250 | 1.02e-53 | 198 |
Find 295 sgRNAs with CRISPR-Local
Find 529 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCATGGTCAGAGGAAG+AGG | 0.416790 | 3_1:+81097553 | Msa0379740:CDS |
| AAACAAGATGAATGCCGTCA+AGG | 0.596474 | 3_1:+81101561 | Msa0379740:CDS |
| AAACCTGCCATTGTTCATTC+TGG | 0.279281 | 3_1:+81099406 | Msa0379740:CDS |
| AAAGACGGCAGTGCCAATCC+TGG | 0.468113 | 3_1:+81098287 | Msa0379740:CDS |
| AAATGCTGCGCCACAATTGA+TGG | 0.346361 | 3_1:+81099456 | Msa0379740:CDS |
| AACCGATGCTTTAGAAACCA+AGG | 0.629910 | 3_1:+81097493 | Msa0379740:CDS |
| AAGTGCAACACAATTGTCTC+AGG | 0.517574 | 3_1:+81097661 | Msa0379740:CDS |
| AATTGGGCTACCATGGCAAA+AGG | 0.539271 | 3_1:+81097617 | Msa0379740:CDS |
| ACAAAGACACCCATTACTGC+TGG | 0.523061 | 3_1:+81100195 | Msa0379740:CDS |
| ACAAAGACACCCATTTATGC+TGG | 0.516867 | 3_1:+81100567 | Msa0379740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAAATGAAGAGTGAAAAAG+TGG | - | chr3_1:81094807-81094826 | None:intergenic | 30.0% | |
| ATGTTTGTGTCTCATATTGT+TGG | + | chr3_1:81095336-81095355 | Msa0379740:intron | 30.0% | |
| ATCAGCTATTTATCCAAACA+GGG | - | chr3_1:81095412-81095431 | None:intergenic | 30.0% | |
| AATCAGCTATTTATCCAAAC+AGG | - | chr3_1:81095413-81095432 | None:intergenic | 30.0% | |
| GAAACAGCTTATAGGTTATA+CGG | - | chr3_1:81095518-81095537 | None:intergenic | 30.0% | |
| TCAAATAAGCCAATACAAAC+AGG | + | chr3_1:81095656-81095675 | Msa0379740:intron | 30.0% | |
| AATTACTTGAATGTGTTGGA+AGG | + | chr3_1:81095809-81095828 | Msa0379740:intron | 30.0% | |
| GAAGTTGAATGCTCAAATAA+AGG | - | chr3_1:81095982-81096001 | None:intergenic | 30.0% | |
| GCATAAAAATACATCCAGTA+TGG | - | chr3_1:81096180-81096199 | None:intergenic | 30.0% | |
| ATGTTCTTATCTTCTGAATG+CGG | - | chr3_1:81096281-81096300 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 81094793 | 81101775 | 81094793 | ID=Msa0379740;Name=Msa0379740 |
| chr3_1 | mRNA | 81094793 | 81101775 | 81094793 | ID=Msa0379740-mRNA-1;Parent=Msa0379740;Name=Msa0379740-mRNA-1;_AED=0.25;_eAED=0.25;_QI=103|0.83|0.85|1|1|1|7|176|1054 |
| chr3_1 | exon | 81094793 | 81094960 | 81094793 | ID=Msa0379740-mRNA-1:exon:20420;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81095043 | 81095238 | 81095043 | ID=Msa0379740-mRNA-1:exon:20421;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81096489 | 81096575 | 81096489 | ID=Msa0379740-mRNA-1:exon:20422;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81097299 | 81097682 | 81097299 | ID=Msa0379740-mRNA-1:exon:20423;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81098266 | 81098437 | 81098266 | ID=Msa0379740-mRNA-1:exon:20424;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81099224 | 81101058 | 81099224 | ID=Msa0379740-mRNA-1:exon:20425;Parent=Msa0379740-mRNA-1 |
| chr3_1 | exon | 81101174 | 81101775 | 81101174 | ID=Msa0379740-mRNA-1:exon:20426;Parent=Msa0379740-mRNA-1 |
| chr3_1 | five_prime_UTR | 81094793 | 81094895 | 81094793 | ID=Msa0379740-mRNA-1:five_prime_utr;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81094896 | 81094960 | 81094896 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81095043 | 81095238 | 81095043 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81096489 | 81096575 | 81096489 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81097299 | 81097682 | 81097299 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81098266 | 81098437 | 81098266 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81099224 | 81101058 | 81099224 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | CDS | 81101174 | 81101599 | 81101174 | ID=Msa0379740-mRNA-1:cds;Parent=Msa0379740-mRNA-1 |
| chr3_1 | three_prime_UTR | 81101600 | 81101775 | 81101600 | ID=Msa0379740-mRNA-1:three_prime_utr;Parent=Msa0379740-mRNA-1 |
| Gene Sequence |
>Msa0379740 ATGCCAGAAAAGAAGTCTAAGAAAGTTACCTTCTCTGAAAACGCCATCGCCACAATCACACAACGGTATGACGTAGCGACTGTGTTGACGGTGTTTCAGGAATTATCACATTACGCTGCTGACGAGAATTTTAATTGGAATGAATTGGTGAAGAAAACAACCACTGGTATTTCTACTGCCAGAGAATATCAGATGCTATGGCGTCACTTGGCGTATGGTTATTCCTTCCCCGAAGATCTCGATCAAGAAGCTCAACCTATGGATGATGATAGTGACCTAGATTGCGAGCTGGAATCATTACCTTCTGGATGTGCGGAATCTACATCAGAGACTTCAGCATGTGTGAAGGTGATAATTGCTTCCCGCACACTAAGTGAATCTACCCCCAGTAGCGCAACAATTGAGGCTCCAATGACTGTAAATTTTCCCGTTTGCCATTCATCACGAACTTCAAAGATTTCGCAGCCCTCTATCTTGATGGATCAGTCAAGCATTATGTTTCCAGTTACTGTCCCGAGACAGACACTGCCAACTGTATCATCAACCGATGCTTTAGAAACCAAGGTAACAATTGGTGGTACCGCAGCTTCCAAAAGGAAAAGAAAAGCATGGTCAGAGGAAGAGGACAATTTGCTACGGGATGCTGTTCAGAAATGGGGTGAAGGGAATTGGGCTACCATGGCAAAAGGAGACAGCTTTCCTATTAAGAGAAGTGCAACACAATTGTCTCAGAGGTGGAGCGCTTTAAGAAAGAAAGACGGCAGTGCCAATCCTGGACCCACCACGACCACTGTGACCACCAACGCACAGTATACTGCCGAACAGTTGGCAACTCGTCACTCCTTAAATTTAGCCCTTGACATGCCATTCAAAAAGTTAACTGCTCCTGGAGTGACTGATCCTGGTAGGACATCCACATCAATAAAAAATCAAGTGCAGCCTCGTAATGCGGCGCAAGTCTCCACAATTCATAGTTCTGTACCACCTCAACGTCCGGCTCAACAAGCTTGTGGTTCCCCTACGAAACCCAAACTGGCCTCTGAAAATTCAGTCTCAAAGTGTAATGCAATGTCAGCTCGTGAGTCAAAACCTGCCATTGTTCATTCTGGAGCACAAACACAAACTGTTTCGCGAACAAATGCTGCGCCACAATTGATGGTTTCGCAAGCAAAAAATGTGGCACATACTGTTCCTGCTGGCAGTACTTTGACAAAGACACCCATTTCTTCTGGTTTGCACCAAAAGATTCGTTCAAATGTGCCTGCTGGTAGTTCTTTGACAAAGACACCCATTTCTACTGGTTTGGCTCCTGTTCAGCCAAATGTAAATGTGCCTGACGGTAGGTCTTTATCAAGGACACCCATTTCTGCTGGTTTACCTTCTAACCAAAAGGTTCATTCAAATGTGCATGTTGGTAGTTCTTTGACGAAGACACCCATTTCTGCTGGTATGCCTTCTAACCAAAAGGTTTATTCAAATGTGCCTGCCGCTAGTTCTTTGACGAAGACACCCAATTCTGCCTGTTTACCTTCTGACCCAAAGGTTCGTTCAAATGTAGCTGCCAGTAGTTCTTTGCCAAAGACACCCATTTCTGCTGGTTTGCCTTCTAACCAAAAGGTTCATTCAAATGTACTTGCTGGTAGTTCTTTGACAAAGCCACCCATTTCTGTTGGTTTGCCTTCTGACCCAAAGGTTCATTCAAATGTGTCTACCGGTAGTTCTTTAACAAAGACACCCATTTCTGCTGGTTTGCCTTCTAACCAAAAGGTTCATTCAAATGTGCCTACTGGTAATTCTTTGACAAAGACACCCATTTCTTCTGGTTTGCCTTCCAACCAAAAGGTTCATTTAAATGTGTCTGCCGGTAATTCTTTGACAAAGACACCCATTACTGCTGGTTTGCCTTCTAATCAAAAGGCTCATTCAAACGTACATGCCGGCAGTTCTTTGACGAAGACACCCATTTCTGTTGGTTTGCCTTCTAACCAAAAGGCTCATTCAAACGTGCCTGCCGGCAGTTCTTTGACGAAGACGCCCATTTCTGTTGGTTTGCCTTCTGACAAAAAGGTTTTAAATGTGCATGCTGCTAGTTCTCTGACAAAGACACCCATTTCTGCTAGTTTGCCTTCTAACCAAAAGGTCCATTCAAATGTACCTGTCGGTAGTTCTTTGACGAAGACACCCATTTCTTCTGGTTTGTCTTCTGACAAGAAGGTTCATTCAAATGTACCTGTTGCTAGTTCTCTGACAAAGACACCCATTTATGCTGGTTTGCCTTCGGACCCAAAGGTTCATCCAAATGTGCCTGCTGGTAATTCTTTGACAAAGACACCCATTTCGGCTGGTTTGCCTTCTGACCCAAAGGTTCATTCAAATGTGCCTGCTGGTAATTCTGTGACAAAGACACCCATTTCGGCTGCTTTGCCTTCTGACCCAAAGGTTCATTCAAATGTGCCTGCTGGTAATACTTTGACAAAGACACCCATTTCCGCTGGTTTGCCTTCCGACCCAAAGGTTCATTCAAATGTGCCTGCTGGTAATTCTTTGACAAAGACACCCATTTCCGCTGGTTTTCCTTCAGACCCAAAAGTTCATTCAAATGTGCCTGCTGGTAATTCTTTGACTAAGACACCCATTTCTGGTTGTCTGCCTTCTGACCCAAAGGTTCATTCAAACGTGCCTGCTGGCAATTCTTTGACAAAGACACCCATTTCCACGGGTTTGCCTTCTGACCAAAAGGATAAACATGTTACCTCTGTGAAAGAAGTGGAAAAAAGACTCGCTGAGCCTGGCTCCACACCAAAAGAGAATGTGAAAGAGGAAGCTTCTACATCTACTACTGAGGGCCAGGTAAATAGCAAACTGGATAAGGCGAGGCTAGATTTAGATAAAGCTAAAAGCATGCCGAGTGAGAAAGTTTTGGAACACAAAGCAGTGCCTCAAAATCCTGTAGGGTGTGAAGAGCGAGGAAGCGTTAAGAGTTCAAATTTTACTCCCAACAAGGCCACTGAGAATGGAAATGACAATTTGAATAAGGAAAGTCAAAATCTAAACCAAGACAAGACAAGTTCTGTTAATGAAAACTCTCAGCATCAGAACGCGAAAGAGAAACTTGTAAATTTGCCAAAACAAGATGAATGCCGTCAAGGTTTGGAAGTGCCGTGA |
| Protein sequence |
>Msa0379740 MPEKKSKKVTFSENAIATITQRYDVATVLTVFQELSHYAADENFNWNELVKKTTTGISTAREYQMLWRHLAYGYSFPEDLDQEAQPMDDDSDLDCELESLPSGCAESTSETSACVKVIIASRTLSESTPSSATIEAPMTVNFPVCHSSRTSKISQPSILMDQSSIMFPVTVPRQTLPTVSSTDALETKVTIGGTAASKRKRKAWSEEEDNLLRDAVQKWGEGNWATMAKGDSFPIKRSATQLSQRWSALRKKDGSANPGPTTTTVTTNAQYTAEQLATRHSLNLALDMPFKKLTAPGVTDPGRTSTSIKNQVQPRNAAQVSTIHSSVPPQRPAQQACGSPTKPKLASENSVSKCNAMSARESKPAIVHSGAQTQTVSRTNAAPQLMVSQAKNVAHTVPAGSTLTKTPISSGLHQKIRSNVPAGSSLTKTPISTGLAPVQPNVNVPDGRSLSRTPISAGLPSNQKVHSNVHVGSSLTKTPISAGMPSNQKVYSNVPAASSLTKTPNSACLPSDPKVRSNVAASSSLPKTPISAGLPSNQKVHSNVLAGSSLTKPPISVGLPSDPKVHSNVSTGSSLTKTPISAGLPSNQKVHSNVPTGNSLTKTPISSGLPSNQKVHLNVSAGNSLTKTPITAGLPSNQKAHSNVHAGSSLTKTPISVGLPSNQKAHSNVPAGSSLTKTPISVGLPSDKKVLNVHAASSLTKTPISASLPSNQKVHSNVPVGSSLTKTPISSGLSSDKKVHSNVPVASSLTKTPIYAGLPSDPKVHPNVPAGNSLTKTPISAGLPSDPKVHSNVPAGNSVTKTPISAALPSDPKVHSNVPAGNTLTKTPISAGLPSDPKVHSNVPAGNSLTKTPISAGFPSDPKVHSNVPAGNSLTKTPISGCLPSDPKVHSNVPAGNSLTKTPISTGLPSDQKDKHVTSVKEVEKRLAEPGSTPKENVKEEASTSTTEGQVNSKLDKARLDLDKAKSMPSEKVLEHKAVPQNPVGCEERGSVKSSNFTPNKATENGNDNLNKESQNLNQDKTSSVNENSQHQNAKEKLVNLPKQDECRQGLEVP* |
